| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| HDAC 3 is homologous to other human HDACs and yeast RPD 3 . ^^^ |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| MTA 1 deregulation in breast cancer cells led to its interactions with the CAK complex components , ER , and HDAC 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Ebp 1 bound HDAC 2 , but not HDAC 1 , in vitro . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Other HDACs , specifically HDAC 2 , 3 , 4 , and 5 , were excluded from such complexes . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In contrast to other HDAC inhibitors VPA also induces proteasomal degradation of HDAC 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Closely related HDAC 1 and HDAC 2 do not elicit humoral response in colon cancer patients . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Recombinant Sin3A bound Ebp 1 directly , but recombinant HDAC 2 failed to bind Ebp 1 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Similar effects were observed for HDAC 2 and , to a lesser extent , for HDAC 1 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Characterization of a human RPD 3 ortholog , HDAC 3 . ^^^ Cloning of the cDNA for a human histone deacetylase ( HDAC 1 ) has shown that it represents a human ortholog of the yeast transcriptional regulator RPD 3 . ^^^ We have screened the expressed sequence tag database ( National Center for Biotechnology Information ) with the yeast RPD 3 sequence and identified a human ortholog of RPD 3 , HDAC 3 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Antibodies directed against endogenous HDAC 1 , HDAC 2 , or HDAC 3 immunoprecipitate histone deacetylase activity that is inhibited in vitro by the small molecule trapoxin ( TPX ) , and all three HDACs are retained by a TPX affinity matrix . ^^^ HDAC 1 and HDAC 2 are associated in HeLa cells in a complex that is predominantly separate from an HDAC 3 immune complex . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| SpHDAC 1 , a cDNA homolog of the yeast Rpd 3 and higher eukaryotic histone deacetylases ( HDAC ) , was cloned from the sea urchin Strongylocentrotus purpuratus . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The histone deacetylase domain of almost all members of higher eukaryotic histone deacetylases already identified ( HDAC family ) is highly homologous to that of yeast RPD 3 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Human HDAC 1 , HDAC 2 , and HDAC 3 proteins are members of the first class , whereas no class 2 human HDAC proteins had been identified . ^^^ Coimmunoprecipitation experiments indicate that these HDAC proteins are not components of the previously identified HDAC 1 and HDAC 2 NRD and mSin3A complexes . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Significantly , upon addition of T 3 , the NRE further recruited the thyroid hormone receptor ( TRbeta ) and another deacetylase , HDAC 2 . ^^^ Supporting the direct interaction between TR and HDAC , in vitro assays showed that TR , through its DNA binding domain , strongly bound to HDAC 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| To date , six HDACs have been identified in mammalian cells : the yeast RPD 3 homologs HDAC 1 , 2 , and 3 and the yeast HDA 1 homologs HDAC 4 , 5 , and 6 . ^^^ |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The class 1 deacetylases HDAC 1 and HDAC 2 are components of multisubunit complexes , one of which could associate with the nuclear hormone receptor corepressor , N CoR . ^^^ In comparison with HDAC 1 and HDAC 2 , HDAC 3 remains relatively uncharacterized , and very few proteins have been shown to interact with HDAC 3 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| HDAC 8 shows a high degree of sequence similarity to HDAC 1 and HDAC 2 and thus belongs to the class 1 of HDACs . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Here we present the complete nucleotide sequence of a cDNA clone , termed HDAC 8 , that encodes a protein product with similarity to the RPD 3 class ( 1 ) of HDACs . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we show that GR acts both as a direct inhibitor of CREB binding protein ( CBP ) associated HAT activity and also by recruiting HDAC 2 to the p 65 CBP HAT complex . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In contrast , N CoR 2 contained predominantly HDAC 1 and HDAC 2 as well as several other subunits that are found in the Sin3A . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments indicate that HDAC 1 and HDAC 2 are associated with Topo 2 in vivo under normal physiological conditions . ^^^ Complexes containing Topo 2 possess HDAC activities , and complexes containing HDAC 1 or HDAC 2 possess Topo 2 activities . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Two genes appear to participate in feedback loops that modulate HDAC activity : ZRT 1 encodes a zinc transporter and is repressed by RPD 3 ( Rpd3p is zinc dependent ) ; BNA 1 encodes a nicotinamide adenine dinucleotide ( NAD ) biosynthesis enzyme and is repressed by SIR 2 ( Sir2p is NAD dependent ) . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In vitro , N CoR can interact with the Sin 3 corepressor , which in turn binds to the histone deacetylase Rpd 3 ( HDAC 1 ) , predicting the existence of a corepressor complex containing N CoR , Sin 3 , and histone deacetylase . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the same HDAC 1 domain is also necessary for in vitro binding of HDAC 2 and HDAC 3 , association with RbAp 48 and for catalytic activity of the enzyme . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The Rpd 3 histone deacetylase ( HDAC ) functions in a large complex containing many proteins including Sin 3 and Sap 30 . ^^^ Based on these observations , we explored whether Pho 23 is a component of the Rpd 3 HDAC complex . ^^^ Furthermore , similar levels of HDAC activity were detected in immunoprecipitates of HA Pho 23 , HA Rpd 3 , or HA Sap 30 . ^^^ In contrast , HDAC activity was not detected in immunoprecipitates of HA Pho 23 or HA Sap 30 from strains lacking Rpd 3 , suggesting that Rpd 3 is the HDAC associated with these proteins . ^^^ However , HDAC activity was detected in immunoprecipitates of HA Sap 30 or HA Rpd 3 from cells lacking Pho 23 , although levels were significantly lower than those detected in wild type cells , indicating that Rpd 3 activity is compromised in the absence of Pho 23 . ^^^ |
|
| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Zea mays ( L . ) histone deacetylase HD 2 was identified as a new type of histone deacetylase ( HDAC ) unrelated to the well known Rpd3p and Hdalp families but with sequence homology to peptidyl prolyl cis trans isomerases ( PPIases ) . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Previous studies have established that YY 1 interacts with histone acetyltransferases p 300 and CREB binding protein ( CBP ) and histone deacetylase 1 ( HDAC 1 ) , HDAC 2 , and HDAC 3 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In addition , of eight histone deacetylases ( HDACs ) tested , only the class 1 HDACs HDAC 1 , HDAC 2 , and HDAC 3 bind ETO . ^^^ Furthermore , ETO 2 binds HDAC 1 , HDAC 2 , and HDAC 3 but also interacts with HDAC 6 and HDAC 8 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The p 65 ( RelA ) subunit of NF kappaB interacts with the histone deacetylase ( HDAC ) corepressors HDAC 1 and HDAC 2 to negatively regulate gene expression . ^^^ Expression of HDAC 1 and HDAC 2 repressed tumor necrosis factor ( TNF ) induced NF kappaB dependent gene expression . ^^^ Consistent with this , we show that HDAC 1 and HDAC 2 target NF kappaB through a direct association of HDAC 1 with the Rel homology domain of p 65 . ^^^ HDAC 2 does not interact with NF kappaB directly but can regulate NF kappaB activity through its association with HDAC 1 . ^^^ Moreover , it suggests that the association of NF kappaB with the HDAC 1 and HDAC 2 corepressor proteins functions to repress expression of NF kappaB regulated genes as well as to control the induced level of expression of these genes . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Furthermore , coimmunoprecipitation experiments indicated that HDAC10v1 associated with HDAC 2 and SMRT ( silencing mediator for retinoid and thyroid hormone receptors ) . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In humans , four highly homologous class 1 HDAC enzymes ( HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 8 ) have been identified to date . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The HDAC domain , homologous to the yeast repressors RPD 3 and HDA 1 , is considered necessary and sufficient for enzymatic activity . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The same regions of the protein that are required for repression physically interact with components of chromatin remodeling complexes , HDAC 1 , HDAC 2 , RbAp46 / 48 , MTA 1 , and MTA 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Similar mechanisms appear to be operative in yeast , in which epistasis experiments have established that the mSin 3 and HDAC orthologs ( SIN 3 and RPD 3 ) , along with a novel protein , SDS 3 , function in the same repressor pathway . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Hyperphosphorylated HDAC 2 was also observed in cells synchronized with nocodazole or taxol , demonstrating regulation of HDAC phosphorylation during mitosis . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In in vitro antimaize HD 2 as well as antimouse HDAC 1 assay , compounds 1a c showed inhibitory activities in the low micromolar range . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The HDAC 1 lysines targeted for modification were identified as C terminal Lys 444 and Lys 476 , which are also present in mammalian HDAC 2 and lower vertebrate HDAC1 / 2 orthologs yet absent from other HDAC family members , pointing to a means of differential regulation among HDAC proteins . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Histone deacetylase 2 ( HDAC 2 ) is a member of a large family of enzymes that alter gene expression by catalyzing the removal of acetyl groups from core histones . ^^^ Histone deacetylase 2 ( HDAC 2 ) is a member of a large family of enzymes that alter gene expression by catalyzing the removal of acetyl groups from core histones . ^^^ Originally isolated as a transcriptional co repressor , HDAC 2 possesses extensive amino acid sequence homology to HDAC 1 ( the founding member and most extensively studied HDAC enzyme ) . ^^^ Because of this high degree of sequence similarity between HDAC 1 and HDAC 2 , coupled with the fact that the two always co exist in the same complexes , it is difficult to assess whether different properties exist between these two proteins . ^^^ We report here that HDAC 2 is a phosphoprotein similar to HDAC 1 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The compounds were tested for inhibition of maize HD 2 , rat liver HDAC , and for the induction of terminal cell differentiation and inhibition of proliferation in Friend leukemic cells . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| A particular pair of HAT ( Esa 1 ) and HDAC ( Rpd 3 ) is proposed to modify the same lysine residue in vitro and in vivo . ^^^ Here we show that HAT ( Esa 1 family ) and HDAC ( Rpd 3 family ) have similar amino acid stretches in the primary structures through evolution . ^^^ We did alanine scanning mutagenesis and found that the ER motif regions of Esa 1 or Rpd 3 are required for HAT activity of Esa 1 or HDAC activity of Rpd 3 , respectively . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| HDAC 2 and NF kappa B p 65 co immunoprecipitated from mesangial cell nuclear extracts , and in vitro translated HDAC 2 specifically interacted with an NF kappa B p 65 GST fusion protein . ^^^ The specific recruitment of HDAC 2 to NF kappa B at target promoters and the consequent effects on acetylation status may play an important role in regulating iNOS as well as other NF kappa B dependent genes involved in inflammation . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| There was no difference in the site of HDAC 1 HDAC6 expression between normal subjects and subjects with asthma , but subjects with asthma had reduced HDAC enzymatic activity and reduced HDAC 1 and HDAC 2 protein expression , as measured by Western blotting . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Although most HDAC 2 is not phosphorylated in the breast cancer cells , HDAC 2 bound to Sp 1 and Sp 3 and cross linked to chromatin in situ is highly enriched in a phosphorylated form that has a reduced mobility in SDS polyacrylamide gels . ^^^ We show that protein kinase CK 2 is associated with and phosphorylates HDAC 2 . ^^^ Alkaline phosphatase treatment of HDAC 2 and Sp 1 and Sp 3 complexes reduced the associated HDAC activity . ^^^ CK 2 phosphorylation of HDAC 2 recruited by Sp 1 or Sp 3 could regulate HDAC activity and alter the balance of histone deacetylase and histone acetyltransferase activities and dynamic chromatin remodeling of estrogen regulated genes . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The mammalian SIN 3 complex consists of histone deacetylases ( HDAC 1 , HDAC 2 ) , several known proteins ( SAP 30 , N CoR ) and as yet unidentified proteins . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| HDAC 1 and HDAC 2 were more strongly inhibited by redFK than HDAC 4 and HDAC 6 . redFK was less active than FK 228 in inhibiting in vivo HDAC activity , due to rapid inactivation in medium and serum . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| DNX was given i . v . in three doses of 80 or 100 mg / m ( 2 ) each ( days 1 3 ) by a 60 min infusion in glucose 5 % , followed by a 4 h infusion of HDAC 2 g / m ( 2 ) ( days 1 5 ) . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Here we report on the cloning of the first P . polycephalum HDAC ( PpHDAC 1 ) related to the S . cerevisiae Rpd 3 protein . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We show that ESET histone methyltransferase associates with histone deacetylase 1 ( HDAC 1 ) and HDAC 2 , and that ESET also interacts with the transcription co repressors mSin3A and mSin3B . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Temporal analysis of IE 1 distribution revealed its initial segregation into ND 10 by binding to PML and / or Daxx and IE 1 dependent recruitment of the transcriptional repressor histone deacetylase 2 ( HDAC 2 ) to this site . ^^^ However , these protein aggregates are dissociated in cells producing sufficient IE 1 through titration of PML , Daxx , and HDAC 2 . ^^^ Importantly , binding of IE 1 to HDAC 2 decreased deacetylation activity . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| By means of quantitative reverse transcription polymerase chain reaction using SYBR Green to detect double stranded DNA , mRNA expression profiles for histone deacetylases ( HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 7 ) , histone acetyltransferases ( GCN 5 and HAT 1 ) , and histone H2A were established . ^^^ The HDAC 1 , HDAC 2 ( class 1 HDAC ) , and HAT 1 ( type B HAT ) revealed similar expression profiles . ^^^ The HDAC 3 ( class 1 HDAC ) tends to have an expression profile similar to those of HDAC 1 , HDAC 2 , and HAT 1 , whereas the HDAC 7 ( class 2 HDAC ) and GCN 5 ( type A HAT ) profiles were different from those three . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The OsHDAC 1 gene encoded a protein of approximately 57 kDa that shared 73 . 5 , 72 . 7 , 79 . 9 , and 57 . 1 % amino acid sequence identity with the OsHDAC 2 , OsHDAC 3 , maize RPD 3 , and human HDAC 1 proteins , respectively . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of these complexes suggests that hCtBP 1 associates with class 1 HDACs , HDAC 1 , HDAC 2 and HDAC 3 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In assays of isolated enzymes , CI 994 inhibited HDAC 1 and HDAC 2 in a concentration dependent fashion but had no effect on the activity of the prototypical histone acetyltransferase GCN 5 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The histone deacetylase inhibitor valproic acid selectively induces proteasomal degradation of HDAC 2 . ^^^ We show that HDAC 2 undergoes basal turnover by the ubiquitin proteasome pathway . ^^^ Valproic acid ( VPA ) , in addition to selectively inhibiting the catalytic activity of class 1 HDACs , induces proteasomal degradation of HDAC 2 , in contrast to other inhibitors such as trichostatin A ( TSA ) . ^^^ Basal and VPA induced HDAC 2 turnover critically depend on the E 2 ubiquitin conjugase Ubc 8 and the E 3 ubiquitin ligase RLIM . ^^^ Ubc 8 gene expression is induced by both VPA and TSA , whereas only TSA simultaneously reduces RLIM protein levels and therefore fails to induce HDAC 2 degradation . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that Sp 1 and Sp 3 recruit HDAC 1 and HDAC 2 , with the latter being phosphorylated by protein kinase CK 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We show here that IkappaBalpha enhances the transactivation potential of several homeodomain containing proteins such as HOXB 7 and Pit 1 through a NF kappaB independent association with histone deacetylase ( HDAC ) 1 and HDAC 3 but not with HDAC 2 , 4 , 5 , and 6 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Here we show that Hop can inhibit SRF dependent transcriptional activation by recruiting histone deacetylase ( HDAC ) activity and can form a complex that includes HDAC 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that subunits of the mSin3A / histone deacetylase 2 ( HDAC 2 ) corepressor complex copurify with hSWI / SNF complexes . ^^^ Using chromatin immunoprecipitation assays , we found that Brg 1 , mSin3A , HDAC 2 , and PRMT 5 are directly recruited to the cad promoter . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Here we examine the interaction of REST , Co REST , Sin3A , HDAC 1 , and HDAC 2 with two archetypical endogenous target genes , the M 4 muscarinic receptor and the sodium type 2 channel ( NaV1 . 2 ) genes . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| To test the hypothesis that the delay reflected a dysfunction of ICP 0 in altering the structure of host protein viral DNA complexes , we examined the state of histone deacetylases ( HDACs ) ( HDAC 1 , HDAC 2 , and HDAC 3 ) . ^^^ We report the following . ( 1 ) HDAC 1 and HDAC 2 , but not HDAC 3 , were modified in infected cells . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We found individual components of Mi2 / NuRD : MBD 3 , Mi 2 , HDAC 1 and HDAC 2 to be expressed from a very early stage of embryo development and to localize in close proximity with each other and with constitutive heterochromatin by the blastula stage . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The LXXLL motif at positions 993 997 of HIRA is necessary for the in vitro interaction with HDAC 2 . ^^^ Moreover , the LXXLL motif is essential for the interaction with endogenous or recombinant HDAC 2 in vivo , probably resulting in formation of the active complex , harboring the HDAC activity . ^^^ Taken together , these results indicate that HIRA should participate differentially in a number of DNA utilizing processes , including transcription repressions , through interactions of its distinct regions with CAF 1p48 and HDAC 2 , respectively . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Most importantly , we demonstrate an interaction between Snail , histone deacetylase 1 ( HDAC 1 ) and HDAC 2 , and the corepressor mSin3A . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We show that the RD 1 domain binds HDAC 1 and HDAC 2 and that HDAC activity is required for PIASy mediated AR repression . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Biological activities of 3 and 4 were predicted by computational tools up to 617 fold more potent than that of 1 against HDAC 1 ; thus , 3 and 4 were synthesized and tested against both mouse HDAC 1 and maize HD 2 enzymes . ^^^ In particular , in mouse HDAC 1 inhibitory assay 3 and 4 were 19 and 6 times more potent than 1 , respectively , and 3 and 4 antimaize HD 2 activities were 16 and 76 times higher than that of 1 , 4 being as potent as SAHA in this assay . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Decreased HDAC 2 activity was associated with tyrosine nitration status . ^^^ Peroxynitrite and SIN 1 , a peroxynitrite generator , were also able to reduce HDAC 2 activity via tyrosine nitration . ^^^ Our data suggest that oxidative stress contributes to worsening inflammation via reduction of HDAC 2 activity through HDAC 2 nitration . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Western blots demonstrated the nickel affinity purified rhHDAC 1 preparation also contained endogenous HDAC 2 and HDAC 3 ; likewise , rhHDAC 3 preparation contained endogenous HDAC 1 and HDAC 2 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation of freshly isolated organs reveals early HDAC 2 occupancy at differentiation gene promoters and corresponding histone hypoacetylation that reverses as HDAC levels fall . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Consistent with prior studies , we find that RPD 3 , which encodes a histone deacetylase ( HDAC ) , is required for repression upon rapamycin treatment . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Northern blot analysis demonstrated two to four fold elevated levels of mRNAs for HDAC 1 , 3 , 5 , and 6 after drug treatment in comparison with untreated cells , while mRNA levels for HDAC 2 and 7 did not change significantly . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| These HDAC inhibitors preferentially inhibited the enzymatic activities of HDAC 1 and HDAC 2 , as compared with the other HDAC isotypes , indicating that class 1 HDAC is the major target of SK 7041 and SK 7068 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| H2O2 and CSC significantly increased acetylation of histone H 4 proteins and were associated with decreased HDAC activity and HDAC 2 levels in A 549 cells . ^^^ Also , the decreased HDAC 2 activity was due to protein modification by aldehydes and nitric oxide products . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We recently reported that development of colonic cancer involves alterations in the transcriptional repression machinery by increased expression of HDAC 2 upon loss of the APC tumor suppressor . ^^^ Increased expression of HDAC 2 is essential for prevention of apoptosis of HT 29 colonic cancer cells . ^^^ We now discuss whether HDAC 2 also plays a role for aberrant cell cycle regulation and expression of the p 21 ( Cip / Waf ) cell cycle inhibitor . ^^^ Whereas inhibition of HDACs by valproic acid or trichostatin A increases p 21 expression , selective interference with HDAC 2 by siRNA transfection or reconstitution of wildtype APC does not affect p 21 expression . ^^^ Likewise , treatment of HT 29 cells with the HDAC inhibitor valproic acid leads to a moderate inhibition of cell cycle progression in the G 1 phase whereas interference with HDAC 2 expression does not . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Using the sos recruitment yeast two hybrid system we found that IRS 1 and histone deacetylase 2 ( HDAC 2 ) interact in the cytoplasmic compartment of yeast cells . ^^^ The interaction mapped to the C terminus of IRS 1 and was confirmed through co immunoprecipitation in vitro of recombinant IRS 1 and HDAC 2 . ^^^ HDAC 2 bound to IRS 1 in mammalian cells treated with phorbol ester or after prolonged treatment with insulin / IGF 1 and also in the livers of ob / ob mice but not PTP1B knockout mice . ^^^ These effects were confirmed using RNA interference against HDAC 2 , indicating that HDAC 2 specifically prevents phosphorylation of IRS 1 by the insulin receptor . ^^^ Specific inhibition of HDAC 2 may increase insulin sensitivity in otherwise insulin resistant conditions . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In the absence of estradiol ( E 2 ) , Sp 1 , Sp 3 , histone deacetylase 1 ( HDAC ) , and HDAC 2 , and low levels of acetylated H 3 and H 4 are associated with the native promoter , with the histones being engaged in dynamic reversible acetylation . ^^^ There is clearance of Sp 1 , but not of Sp 3 , from the promoter while HDAC 1 and HDAC 2 remain bound . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The Dnmt3b mediated differentiation was attributed to its N terminal domain , which recruits histone deacetylase 2 ( Hdac 2 ) , as demonstrated by ( 1 ) impeding of differentiation by the Hdac inhibitors , ( 2 ) facilitation of the differentiation process by overexpression of the N terminal domain of Dnmt3b , ( 3 ) higher Hdac activity associated with Dnmt3b after NGF treatment , and ( 4 ) coimmunoprecipitation and cosedimentation of Dnmt3b specifically with Hdac 2 in a glycerol density gradient . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The yeast histone acetyltransferase ( HAT ) gene gcn 5 and histone deacetylase ( HDAC ) gene rpd 3 were cloned from yeast genomic DNA by PCR amplification . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| TSA treatment increased the acetylation of the transcription factors Sp 1 and C / EBPalpha and decreased their binding as well as the binding of CBP and HDAC 2 to the bcl 2 promoters . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Cyclin D 1 bound HDAC in vivo and preferentially physically associated with HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 5 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| However , in response to dopamine , histone deacetylase HDAC 2 and corepressor mSin3A were rapidly recruited to the prolactin promoter , and association was sustained above basal levels over a 1 h period . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Corticosteroids switch off inflammatory genes in asthma through the inhibition of HAT activity and by the recruitment of HDAC 2 to the activated inflammatory gene complex . ^^^ In chronic obstructive pulmonary disease , there is a reduction in HDAC 2 activity and expression , which may account for the amplified inflammation and resistance to the actions of corticosteroids . ^^^ The reduction in HDAC 2 may be secondary to oxidative and nitrative stress as a result of cigarette smoking and severe inflammation , and may also occur in severe asthma , smoking asthmatic patients and cystic fibrosis . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| During development in mouse tissues , histone acetylation levels decreased and associated HDAC 2 levels increased at the region closest to the transcriptional start site , correlating with a 40 60 % decrease in SMN transcript and protein levels . ^^^ HDAC 2 , in particular , may be a future therapeutic target for SMA . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Among different iso enzymes of HDAC and sirtuins grouped as the HDAC super family , little is known as to how histone deacetylase 2 ( HDAC 2 ) causes carcinogenesis in solid tumors . ^^^ Here , in order to investigate the possible role of HDAC 2 in gastric carcinogenesis , we analyzed the expression of HDAC 2 in 71 gastric adenocarcinomas by immunohistochemistry . ^^^ Moderate to strong expression of HDAC 2 was found in 44 ( 62 % ) out of a total of 71 tumors . ^^^ The majority of positive tumors , which were detected in the nucleus but not in normal gastric epithelium , did not express HDAC 2 or showed only weak positive staining . ^^^ Interestingly , we also noted that HDAC 2 expression appeared to be associated with tumor aggressiveness as HDAC 2 expression was observed to be statistically significant in advanced gastric cancer ( P=0 . 0023 , Chi square test ) and in positive lymph node metastasis ( P=0 . 0713 , Chi square test ) . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Expression of HDAC 2 protein was quantified with the use of Western blotting . ^^^ The mRNA expression of HDAC 2 , HDAC 5 , and HDAC 8 and expression of the HDAC 2 protein were also lower in patients with increasing severity of disease . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In particular , PIASx ( alpha ) facilitates the loss of the repressive HDAC 2 from sumoylated Elk 1 , a key event in the activation of Elk 1 in response to signalling through the ERK MAP kinase pathway . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| HDAC 3 but not HDAC 1 or HDAC 2 was required for AP 1 mediated stimulation of c jun expression . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| In the remaining seven girls we then analyzed the genes HDAC 1 , HDAC 2 , and HDAC 8 that encode for the histone deacetylases 1 , 2 , and 8 which interact with MeCP 2 and are essential for its function . ^^^ The genes HDAC 1 , HDAC 2 , and HDAC 8 do not seem to play a role in the pathogenesis of RTT and at least in our subgroup no mutations in the CDKL 5 gene were detected . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We now report that Sp 3 , CAGA element binding proteins , and a corepressor complex consisting of mSin3A , histone deacetylase ( HDAC ) 1 , and HDAC 2 associates with a transcriptional repressor region within the mouse StAR promoter . 5 ' Promoter deletion analysis localized the negative regulatory region between 180 and 150 bp upstream of the transcription start site , and mutations in both the CAGA and Sp binding elements were required to relieve the repression of basal StAR promoter activity . ^^^ Coimmunoprecipitation analysis identified the presence of the mSin3A , HDAC 1 , and HDAC 2 corepressor complex in MA 10 cells . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| To understand the physiological functions of HDACs , we characterized six different Drosophila HDACs , including Rpd 3 , HDAC 3 , HDAC 4 , HDAC 6 S , HDAC 6 L , and Sir 2 , by developmental expression pattern , transcriptional profiles of target genes , and sensitivity to HDAC inhibitors . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The HDAC inhibitor , trichostatin A , reduced MMSET 1 repression activity and in vitro co immunoprecipitation analyses indicated that MMSET 1 specifically recruits HDAC 1 and mSin3b , but not HDAC 2 or HDAC 4 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| To understand the difference between class 1 HDAC isoforms that could be exploited for the design of isoform specific HDAC inhibitors , we have built three dimensional models of four class 1 histone deacetylases , HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 8 . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Biologic activity of VPA was confirmed by serial analysis of HDAC 2 protein levels in peripheral blood ( PB ) mononuclear cells . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Corticosteroids , the most effective anti inflammatory drugs so far available , recruit HDAC 2 to activated inflammatory gene complexes through an interaction with glucocorticoid receptor and thus switch off activated inflammatory genes . ^^^ In chronic obstructive pulmonary disease and in asthmatic patients who smoke , there is a reduction in HDAC 2 activity and expression , resulting in amplification of inflammation and corticosteroid resistance . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Specific knockdown of HDAC 2 by RNA interference resulted in reduced sensitivity to dexamethasone suppression of interleukin 1beta induced granulocyte / macrophage colony stimulating factor production . ^^^ Loss of HDAC 2 did not reduce GR nuclear translocation , GR binding to glucocorticoid response element ( GRE ) on DNA , or GR induced DNA or gene induction but inhibited the association between GR and NF kappaB . ^^^ GR becomes acetylated after ligand binding , and HDAC 2 mediated GR deacetylation enables GR binding to the NF kappaB complex . ^^^ In conclusion , we show that overexpression of HDAC 2 in glucocorticoid insensitive alveolar macrophages from patients with COPD is able to restore glucocorticoid sensitivity . ^^^ Thus , reduction of HDAC 2 plays a critical role in glucocorticoid insensitivity in repressing NF kappaB mediated , but not GRE mediated , gene expression . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Histone deacetylases ( HDACs ) suppress inflammatory gene expression , but their activity and expression ( particularly of HDAC 2 ) is reduced in the peripheral lung and in alveolar macrophages of patients with COPD . ^^^ This results in amplification of the inflammatory response as COPD progresses but also accounts for corticosteroid resistance in COPD , since HDAC 2 is required by corticosteroids to switch off activated inflammatory genes . ^^^ The reduction in HDAC 2 appears to be secondary to the increased oxidative and nitrative stress in COPD lungs . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| CSE also reduced histone deacetylase ( HDAC ) activity and HDAC 1 , HDAC 2 , and HDAC 3 protein levels . ^^^ This was associated with posttranslational modification of HDAC 1 , HDAC 2 , and HDAC 3 protein by nitrotyrosine and aldehyde adduct formation . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that this marker of functional HDAC inhibition occurs almost immediately ( 15 min ) after exposure of F 9 cells to VPA , whereas no influence on the HDAC protein levels ( HDAC 2 and HDAC 3 ) could be detected even after 24 h of treatment . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Hop functions through interactions with histone deacetylase ( HDAC ) 2 to mediate repression of cardiac specific genes , and recent studies show that HDAC activity and HDAC 2 expression are decreased in people with chronic obstructive pulmonary disease . ^^^ Here , we show that Hop is expressed in airway epithelium coincident with HDAC 2 , and expression is induced by the combination of dexamethasone and cAMP in parallel with induction of surfactant protein gene expression . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Association of histone deacetylase 2 ( Hdac 2 ) with T Cad promoter and restoration of the promoter activity from Dnmt3b mediated suppression upon treatment with Hdac inhibitor indicated involvement of histone deacetylation in this process . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| This is exemplified by the transcription factor Elk 1 , where HDAC 2 is specifically recruited in response to sumoylation . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Induction of the methylbinding proteins was accompanied with enhanced HDAC 2 labeling intensity and mRNA synthesis in response to fluoxetine . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| CRA 024781 inhibited pure recombinant HDAC 1 with a K ( 1 ) of 0 . 007 mumol / L , and also inhibited the other HDAC isozymes HDAC 2 , HDAC3 / SMRT , HDAC 6 , HDAC 8 , and HDAC 10 in the nanomolar range . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| A new series of 2 , 3 , and 4 acylaminocinnamyl N hydroxyamides 1 3 have been prepared , and their anti HDAC ( against maize HD 2 , HD 1 B , and HD 1 A enzymes ) activities have been assessed . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Brg 1 was associated with increased occupancy of the P4 . 2 promoter by the nuclear co repressor mSin3A and HDAC 2 ( histone deacetylase 2 ) and with reduced histone H 3 and H 4 acetylation . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Using different HDACi together with small interfering RNA for HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 6 , we report that inhibition of HDAC 1 and HDAC 2 but not HDAC 3 , HDAC 6 , and HDAC 8 are primarily responsible for sensitization to TRAIL induced apoptosis . ^^^ Based on these data and our previous studies , we propose that a clinical trial in CLL is warranted using a combination of a selective HDACi that inhibits HDAC 1 and / or HDAC 2 together with a form of TRAIL that signals through TRAIL receptor 1 . . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| Furthermore , apicidin activation of NF kappaB seems to result from HDAC 1 inhibition , as evidenced by the observation that overexpression of HDAC 1 , but not HDAC 2 , 3 or 4 , dramatically inhibits NF kappaB reporter gene activity . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| CRA 026440 inhibited pure recombinant isozymes HDAC 1 , HDAC 2 , HDAC3 / SMRT , HDAC 6 , HDAC 8 , and HDAC 10 in the nanomolar range . ^^^ |
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| Interacting proteins: Q9UKV0 and Q92769 |
Pubmed |
SVM Score :0.0 |
| The benzene ring of 2 was substituted with a wide range of electron donating and electron withdrawing groups , and the effect was evaluated on three HDACs from maize , namely HD 2 , HD 1 B ( a class 1 HDAC ) , and HD 1 A ( a class 2 HDAC ) . ^^^ |
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