Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Here , we demonstrate that the catalytic subunit of mouse cdc2 / cdk2 CAK ( a 39 kDa protein designated p39MO15 ) can assemble with a regulatory protein present in either insect or mammalian cells to generate a CAK activity capable of phosphorylating and enzymatically activating both cdk 2 and cdk 4 in complexes with their respective cyclin partners . ^^^ Morgan , Cell 78 : 713 724 , 1994 ] ) can assemble with p39MO15 to activate both cyclin A cdk 2 and cyclin D cdk 4 in vitro , implying that CAK is structurally reminiscent of cyclin cdk complexes themselves . ^^^ Antisera produced to the p39MO15 subunit can completely deplete mammalian cell lysates of CAK activity for both cyclin A cdk 2 and cyclin D cdk 4 , with recovery of activity in the resulting immune complexes . ^^^ By using an immune complex CAK assay , CAK activity for cyclin A cdk 2 and cyclin D cdk 4 was detected both in quiescent cells and invariantly throughout the cell cycle . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The expression of p 27 , cyclin D 1 , and the activities of Cdk 4 and Cdk activating kinase ( CAK ) , composed of Cdk 7 and cyclin H , were not affected . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Interaction between MyoD and cdk 4 disrupts MyoD DNA binding , muscle specific gene activation and myogenic conversion of 10T1 / 2 cells independently of cyclin D 1 and the CAK activation of cdk 4 . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Cdk 4 derived from early passage quiescent cells was effectively activated by incubation with cyclin D 1 and Cdk activating kinase ( CAK ) in vitro , whereas Cdk 4 from senescent cells was not . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Consistent with previous reports , CAK strongly phosphorylated Cdk 2 , Cdk 4 , CTD and intact PolII . ^^^ The kinase activity of TFIIH displayed stronger substrate preference for Cdk 4 than did CAK . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The Cyclin dependent kinase ( CDK ) Activating Kinase ( CAK ) is responsible for the activating phosphorylation of CDK 1 , CDK 2 , CDK 4 and CDK 6 and regulation of the cell cycle . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Complexes between cyclin D 1 and cyclin dependent kinase 4 ( cdk 4 ) assemble in growth arrested cells , but cdk 4 is not phosphorylated in vivo by the cdk activating kinase ( CAK ) and remains inactive . ^^^ Levels of the cdk inhibitor p27Klp1 increase in cAMP treated cells , and its immunodepletion from inhibitory lysates restores CAK mediated cdk 4 activation . ^^^ Kip 1 does not bind to CAK , but its association with cyclin D cdk 4 prevents CAK from phosphorylating and activating the holoenzyme . . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Assembly of cyclin D cdk 4 complexes in coinfected Sf 9 cells facilitates phosphorylation of cdk 4 on threonine 172 by a cdk activating kinase ( CAK ) . ^^^ Assembly can proceed in the absence of this modification , but cdk 4 mutants which can not be phosphorylated by CAK remain catalytically inactive . ^^^ Therefore , formation of the cyclin D cdk 4 complex and phosphorylation of the bound catalytic subunit are independently regulated , and in addition to the requirement for CAK activity , serum stimulation is required to promote assembly of the complexes in mammalian cells . . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
CDK 4 in complexes with mutant cyclin D 1 ( T156A or T156E but not T156S ) is not phosphorylated by recombinant CDK activating kinase ( CAK ) in vitro , fails to undergo activating T loop phosphorylation in vivo , and remains catalytically inactive and unable to phosphorylate the retinoblastoma protein . ^^^ CAK phosphorylation is not required for nuclear transport of cyclin D 1 CDK4 complexes , because complexes containing wild type cyclin D 1 and a CDK 4 ( T172A ) mutant lacking the CAK phosphorylation site are efficiently imported . ^^^ These results suggest that cyclin D 1 ( T156A or T156E ) forms abortive complexes with CDK 4 that prevent recognition by CAK and by other cellular factors that are required for their nuclear localization . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
This effect was associated with reduction of cyclin D 1 , cdk 4 and CDK activating kinase ( cyclin H and cdk 7 ) protein in HSC 3 cells . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Here , the nuclear translocation of these complexes strictly correlated in individual cells with the enhanced presence of nuclear p 27 . p 27 , like cyclin D 3 , supported the TSH stimulated pRb kinase activity of the CDK 4 complex and , as demonstrated using the high resolution power of the two dimensional ( 2D ) gel electrophoresis , the phosphorylation of CDK 4 , presumably by the nuclear CDK activating kinase . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
In the glioblastomas with no alterations of CDKN2A , CDK 4 or RB 1 , several other genes ( CCND 1 , CCND 2 , CCND 3 , CDK 6 , E2F , CDK 7 , MYC and MYCN ) whose products take part in cell cycle regulation were examined . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Phosphorylation of the cyclin D 1 bound Cdk 4 by Cdk 4 activating kinase , composed of cyclin H and MO 15 ( alias Cdk 7 ) , which was reconstituted in Spodoptera frugiperda cells ( Sf 9 ) could convert inactive cyclin D 1 Cdk4 complex into active form in vitro , suggesting that threonine 172 in the Cdk 4 , whose phosphorylation is required for its activation , was in part unphosphorylated in the contact inhibited 3Y1 . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
We now demonstrate that the abnormal fiber regions in MFM immunoreact strongly for ( a ) CDC 2 kinase , the mitotic kinase that phosphorylates and disassembles intermediate filaments ; ( b ) cyclin dependent kinases CDK 2 , CDK 4 , and CDK 7 , which are involved in regulation of the cell cycle ; ( c ) lamin B , which normally supports the inner nuclear membrane ; and ( d ) the nuclear matrix associated protein . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Immunoaffinity purified Cak 1 phosphorylated monomeric Cdc 2 and Cdk 2 , but not Cdk 4 ; the phosphorylation of both Cdc 2 and Cdk 2 were increased in the presence of recombinant cyclin A . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Phosphorylation of the cyclin D 1 bound Cdk 4 by the Cdk activating kinase could convert the inactive cyclin D 1 Cdk4 complex into its active form in vitro , suggesting that threonine 172 of the Cdk 4 , of which phosphorylation is required for its activation , was in part unphosphorylated in contact inhibited 3Y1 cells . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The targeted degradation of MyoD following CDK phosphorylation identifies a mechanism through which MyoD activity can be regulated coordinately with the cell cycle machinery ( CDK 2 and CDK 4 ) and / or coordinately with the cellular transcriptional machinery ( CDK 7 , CDK 8 , and CDK 9 ) . . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The present study was undertaken to explore the expression and regulation of six additional members of the Cdk family ( Cdk 2 , Cdk 3 , Cdk 4 , Cdk 6 , Cdk 7 and Cdk 8 ) during lens differentiation . ^^^ In the lens epithelium , we detected proteins and mRNAs corresponding to all other Cdks examined ( Cdk 2 , Cdk 4 , Cdk 7 , Cdk 8 ) throughout this developmental period . ^^^ Epithelial cells showed significant Cdk 2 activity , which decreased with developmental age , but no significant activity was detected for Cdk 4 , Cdk 7 , or Cdk 8 . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
However , RA had no effect on the levels of cyclins A , D 1 , D 3 , E , or H , or on Cdk 2 , Cdk 4 , Cdk 5 , CDk 6 , Cdk 7 , p 16 ( Ink4A ) , p 15 ( Ink4B ) , p 53 , or pRb proteins in CH 27 cells . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Enzymatic activities of cyclin D 1 , E , A , Cdk 4 , Cdk 6 , Cdc 2 , Cdk 7 , and Wee 1 kinase were measured by in gel kinase assay . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
These results suggest that p 16 ( INK4A ) may regulate cell cycle progression by inhibiting not only CDK 4 pRb kinase activity but also by modulating CDK 7 CTD kinase activity . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
In contrast , neither CDK 4 nor CDK 7 itself can phosphorylate the CDK 7 T loop in vitro . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
In contrast , lack of adhesion to substratum decreased cyclin H but not cdk 7 with accumulation of a slower migrating , presumably unphosphorylated cdk 4 isoform . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
In contrast , neuronal expression of cdk 7 and cytoplasmic cdk 4 decreased during explant cultivation , whereas cdk 3 was detected in the same small percentage of neurons before and after explant cultivation and cdks 1 , 5 , and 6 were not detected in neuronal cell bodies . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The protein levels of cyclins D 1 , E , A , and H , and of cyclin dependent kinase 1 ( Cdk 1 ) , Cdk 2 , Cdk 4 , Cdk 6 , and Cdk 7 in HCC and in surrounding nontumorous cirrhosis were determined by Western blot . ^^^ The enzymatic activities of cyclins D 1 , E , A , Cdk 1 , Cdk 4 , Cdk 6 , Cdk 7 , and Wee 1 were measured using in vitro kinase assays . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Although cell cycle analyses were performed by means of computer assisted microscope analyses of Feulgen stained nuclei , the gastrin induced effects of the levels of expression of cyclins D 3 and E , CDK 2 , CDK 4 , CDK 5 , CDK 7 , p 15 , p 16 , E2F1 , and E2F2 were assayed by means of quantitative Western blot test . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Among them , compound 20 displayed high inhibitory potency at CDK 1 ( IC ( 50 ) = 0 . 021 microM ) , CDK 2 , and CDK 5 and submicromolar potency at CDK 4 , CDK 6 , and CDK 7 . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
The latter cells were the most responsive to betulinic acid , showing a selective decline in the cdk 4 protein , without a comparable change in other key cell cycle proteins like cdc 2 , cdk 2 , cdk 7 or cyclin A , prior to apoptosis associated PARP fragmentation . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
Furthermore , down regulation of CDK 2 , CDK 4 , and cyclin D 1 was observed in PC 3 cells treated with 1 , 000 nmol / L of MONCPT , whereas overexpression of CDK 7 , CDK 1 , and cyclin B 1 was seen in PC 3 cells treated with 10 and 100 nmol / L of MONCPT . ^^^
Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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Interacting proteins: P50613 and P11802	Pubmed	SVM Score :0.0
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