| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic cyclin D 1 assembled with cyclin dependent kinase 4 ( CDK 4 ) , and the CDK inhibitors p21Cip1 and p27Kip1 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The induced cyclin D 1 formed a complex with cyclin dependent kinase 4 ( CDK 4 ) and p 21 ( Cip 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| However , in these same cells the association of cdk 4 with cyclin D 1 , PCNA , and Waf 1 is disrupted . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Interactions of cyclin D 1 , Cdk 4 , and p21Cip1 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| There was no effect on p 21 ( Cip 1 ) , cyclin D 2 , cdk 4 , and cdk 6 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| TGFbeta 1 induced growth arrest was associated with notably increased binding of p 21 ( WAF 1 ) to cdk 4 and cdk 6 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| No changes in protein level were observed for CDK 2 , CDK 4 , p 21 ( cip 1 ) , or p 15 ( INK4B ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| HGF treatment of the cells led to a redistribution of p 21 ( CIP 1 ) and p 27 ( KIP 1 ) from Cdk 4 to Cdk 2 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :1.0149069 |
| Conversely , CDK 4 stably associates with both p 21 ( CIP 1 ) and p 27 ( KIP 1 ) in cyclin containing complexes , suggesting that CDK 4 is in equilibrium between INK 4 and p 21 ( CIP 1 ) or p 27 ( KIP 1 ) bound states . 1.0149069^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Abundant cyclin dependent kinase 4 ( Cdk 4 ) , a protein related functionally to p21 / Waf 1 , also was present in the cyst . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Semi quantitative RT PCR and Western blot analysis showed no change in the expression level of cyclin dependent kinase 4 ( CDK 4 ) , p 16 ( Ink4a ) or p 21 ( Cip 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , p27Kip1 ( where Kip is kinase inhibitory protein ) was induced markedly , whereas other negative cell cycle regulators , such as p21Cip1 ( where Cip is cyclin dependent kinase interacting protein ) , p15INK4B and p16INK4A ( where INK is inhibitors of cyclin dependent kinase 4 ) , were not , implying its association in the G 1 arrest . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In HepG 2 cells , this inhibition was associated with an arrest of the cell cycle in G ( 0 ) G ( 1 ) , increased cellular levels of p 21 ( CIP 1 ) , decreased levels of the hyperphosphorylated form of the retinoblastoma protein , and decreased levels of cyclin D 1 , but no significant changes were seen in the levels of the p 16 ( INK4a ) , p 27 ( KIP 1 ) , cyclin dependent kinase 4 , cyclin dependent kinase 6 , glycogen synthase kinase 3beta , or beta catenin proteins . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| A 20 amino acid peptide based on the carboxy terminal domain of p 21 ( WAF 1 ) inhibits Cdk 4 activity with a concentration for half maximal inhibition ( l0 . 5 ) of 46 nM , and it is only four fold less active than the full length protein . ^^^ These data support a physiological role for the carboxyl terminus of p 21 ( WAF 1 ) in the inhibition of Cdk 4 activity . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The expression of cyclins ( A , B 1 , D 1 , D 3 , E ) , cyclin dependent kinases ( CDK 2 ( 3 ) , CDK 4 ) , and the cyclin dependent kinase inhibitors ( CDKIs ) p 16 ( INK4A ) and p 21 ( CIP 1 ) was studied in 9 malignant human astrocytoma cell lines using northern blot analysis , immunocytochemistry , and immunoblotting to see if their altered expression contributed to astrocytoma proliferation . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| As cdk 4 and cdk 6 associated p15INK4B increased during TGF beta arrest of sensitive cells , there was a loss of cyclin D 1 , p21Cip1 , and p27Kip1 from these kinase complexes , and cyclin E cdk 2 associated p27Kip1 increased . ^^^ In HMEC , p15INK4B complexes did not contain detectable cyclin . p15INK4B from both sensitive and resistant cells could displace in vitro cyclin D 1 , p21Cip1 , and p27Kip1 from cdk 4 isolated from sensitive cells . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The amount of Cdk 4 that is bound to p21Cip1 increases rapidly after addition of dexamethasone , and the activity of Cdk 4 pRb kinase decreases in parallel . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| We found that status of p 107 , p 130 , p15INK4b , p18INK4c , p21Cip1 , p27Kip1 , cyclin D 1 , and Cdk 4 were not correlated with the growth inhibitory activity of exogenous p16INK4a . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In contrast , enforced overexpression of the CDK inhibitor p21Cip1 together with mutant cyclin D 1 ( T156A ) CDK 4 complexes enhanced their nuclear localization . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Endothelin 1 stimulated [ 3H ] thymidine incorporation , CDK 4 kinase activity , and the percent of cells in S phase were found to be significantly inhibited by overexpression of p16INK4 and slightly inhibited by overexpression of p21cip1 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that cyclin D 1 , cdk 4 and their association act as promoting factors , and that both p 21 ( CIP 1 ) and p 27 ( KIP 1 ) may have physiological functions as adaptor proteins in additions to their roles as CDK inhibitors in rat liver regeneration . . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Twenty primary central nervous system lymphomas ( PCNSL ) from immunocompetent patients ( nineteen B cell lymphomas and one T cell lymphoma ) were investigated for genetic alterations and / or expression of the genes BCL 2 , CCND 1 , CDK 4 , CDKN1A , CDKN2A , MDM 2 , MYC , RB 1 , REL , and TP 53 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The protein levels of cyclin dependent kinase 2 ( cdk 2 ) and cdk 4 were unaffected during this G 1 arrest and the total cellular levels of the cdk inhibitors p21cip1 and p27kip1 were not increased . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Likewise , cyclin D is complexed with its catalytic partners CDK 4 and CDK 6 in senescent HDF , but it is not known whether these complexes are active . p21Sdi1 , Cip 1 , Waf 1 , a ubiquitous inhibitor of the activity of cyclin CDK complexes , increases progressively throughout the life span of HDF , but then declines again after the cells become senescent . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Thus , p 21 [ CIP 1 ] and p 27 [ KIP 1 ] , act in concert to inhibit cyclin E / CDK2 activity which , together with CDK 4 inactivation , confers a G 1 phase arrest . . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Thus , upon the induction of p 16 ( INK4a ) , p 27 ( KIP 1 ) appears to switch its allegiance from CDK 4 to CDK 2 , and the accompanying reassortment of components leads to the inhibition of cyclin E CDK 2 by p 27 ( KIP 1 ) and p 21 ( CIP 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The widely prevailing view that the cyclin dependent kinase inhibitors ( CKIs ) are solely negative regulators of cyclin dependent kinases ( CDKs ) is challenged here by observations that normal up regulation of cyclin D CDK 4 in mitogen stimulated fibroblasts depends redundantly upon p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Western blots showed that the expression of cell cycle dependent kinases ( cdk 2 and cdk 4 ) , cyclin D 1 and p 53 was significantly reduced , while WAF 1 was increased , after BFA treatment . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| CDK 4 kinase activities were unaffected , as were the levels of the CDK inhibitor p21Cip1 present in cyclin E immunocomplexes . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Induction of p19ARF activated p 53 by increasing its stability , and allowed the expression of p21Cip1 , which bound to all of the cyclin D 1 cdk complexes ( cyclin D 1 cdk2 , cdk 4 , and cdk 6 ) thereby inhibiting their kinase activities . p19ARF formed complexes with several cellular proteins including mouse MDM 2 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Cdk 4 activity is also significantly decreased in the treated cells and is accompanied by an increased expression of the Cdk inhibitor p 21 ( CIP 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The dissociation of p 21 ( CIP 1 ) and p 27 ( KIP 1 ) from their cdk complexes correlated well with the activation of cdk 2 , cdk 4 , and cdk 6 and the release from cell cycle arrest . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| TPX activated p 21 ( waf 1 ) transcription that led to elevated p 21 ( waf 1 ) protein levels in three human tumor cell lines without altering the protein levels of cdk 2 , cdk 4 , or cyclin B . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| As already reported , oncogenic Ras expression was sufficient to induce cyclin D 1 and p21cip1 expression and their association with cdk 4 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| We hypothesized that a variation in the inactivation of cdk 2 and cdk 4 during the G ( 1 ) phase of the cell cycle by p 27 ( Kip 1 ) , p 21 ( Cip 1 ) , and p 16 ( Ink 4 ) leads to different effects on VSMC growth in vitro and in vivo . ^^^ METHODS AND RESULTS : The expression of p 27 ( Kip 1 ) and p 21 ( Cip 1 ) in serum stimulated VSMCs inactivated cdk 2 and cdk 4 , leading to G ( 1 ) growth arrest . p 16 ( Ink 4 ) inhibited cdk 4 , but not cdk 2 , kinase activity , producing partial inhibition of VSMC growth in vitro . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| To better understand these mechanisms in long term cultured lymphocytes we have characterized two human long term cultured IL 2 dependent T cell lines regarding telomere length , telomerase activity , and the expression of selected cell cycle regulators ( pRb , p 53 , cyclin E , cyclin D 1 , cyclin D 2 , cyclin D 3 , cdk 4 , p 16 ( INK4a ) , p 21 ( WAF 1 ) , p 27 ( KIP 1 ) , c myc , bcl 2 , and NPAT ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Degradation is mediated through a previously unrecognized destruction box in cyclin D 1 and leads to a release of p21cip1 from CDK 4 to inhibit CDK 2 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Overexpression of wild type waf 1 in melanoma cells reduced growth of subconfluent cells , decreased Cdk 4 activity with a concomitant increase in hypophosphorylated Rb , and promoted cell death by apoptosis . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Both p 21 ( Cip 1 ) and p 27 ( Kip 1 ) proteins were induced during erythroid differentiation , but only p 27 ( Kip 1 ) associated with the principal G ( 1 ) CDKs cdk 4 , cdk 6 , and cdk 2 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Treatment with salicylate prevented PDGF induced downregulation of p 21 ( Waf 1 ) and p 27 ( Kip 1 ) but not of the Cdk 4 / 6 inhibitor p 16 ( Ink 4 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In p 27 ( kip 1 ) p 21 ( cip 1 ) deficient cells , the cyclin D 3 pool consisted primarily of cyclin D 3 monomers , whereas in wild type cells , the majority of cyclin D 3 molecules were complexed to cdk 4 and either p 27 ( kip 1 ) or p 21 ( cip 1 ) or were monomeric . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| We found that the expression of cyclin A and p 21 ( WAF 1 ) molecules was primarily modulated by TGFbeta 1 treatment while the expression of other regulatory components , like cyclins D , cyclin E , cdk 2 , cdk 4 , and cdk 6 or p 15 ( INK4B ) , p 16 ( INK4A ) , and p 27 ( KIP 1 ) was not significantly affected . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| At 300 microM , N BPs reduced expression of cyclin dependent kinase ( cdk ) 2 and cdk 4 and enhanced expression of p 21 ( waf 1 ) and p 27 ( kip 1 ) and their binding to cdks with corollary hypophosphorylation of retinoblastoma . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Of the cell cycle control proteins , PCNA , Cdk 2 , and cyclin A were detected at high concentrations ; cdc 2 , Cdk 4 , and cyclin B were detected at very low concentrations ; while cyclin D 1 , cyclin D 3 , Cip 1 , and Kip 1 were not detected . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In addition , phosphorylation of p 21 ( Cip 1 ) at Thr 145 decreases the binding of the cyclin dependent kinases Cdk 2 and Cdk 4 to p 21 ( Cip 1 ) and attenuates the Cdk 2 inhibitory activity of p 21 ( Cip 1 ) . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| P 21 ( Cip 1 ) induced by Raf is associated with increased Cdk 4 activity in hematopoietic cells . ^^^ Raf activation increased the expression of Cdk 2 , Cdk 4 , cyclin A , cyclin D , cyclin E , p 21 ( Cip 1 ) and c Myc and decreased the expression of p 27 ( Kip 1 ) which are associated with G ( 1 ) progression . ^^^ The cell clones with the highest Delta Raf activity , FD / Delta B Raf : ER , underwent apoptosis before cell proliferation . p 21 ( Cip 1 ) induced by Raf activation specifically bound with Cdk4 / cyclin D complexes but not Cdk2 / cyclin E complexes and this binding was associated with the increased Cdk 4 activity . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| A second , more indirect role for CDK 4 is in late G 1 , where it may sequester the inhibitors p27KIP1 or p21CIP1 away from CDK 2 , and in doing so upregulate the CDK 2 activity necessary for cells to proceed completely through G 1 into S phase . ^^^ As the pivotal residues around the most predominant R24C activating CDK 4 mutation are invariant between CDK 2 and CDK 4 , we speculated that the pivotal arginine ( position 22 in CDK 2 ) , or a nearby residue , may be mutated in some melanomas , resulting in the diminution of its binding and inhibition by p27KIP1 or p21CIP1 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| METHODS : We performed Western blot analysis of five cell cycle stimulating ( cyclins D 1 , E , B 1 , cdk 2 , cdk 4 ) and three cell cycle inhibiting ( p 16 ( INK4a ) , p 21 ( WAF 1 ) , Rb ) proteins in 41 endometrial carcinoma specimens . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| WAF 1 ) is associated with CDK 2 and CDK 4 protein during HL 60 cell differentiation by TPA treatment . ^^^ Increased p 21 ( WAF 1 ) is associated with CDK 2 and CDK 4 . pRb is dephosphorylated in the presence of p 21 CDK2 / 4 complexes , and the Rb E2F1 complex increases after TPA treatment , whereas the Rb HDAC 1 complex decreases slightly . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Expression of cell cycle regulatory proteins , such as cyclin D 1 , cyclin E , cdk 2 , cdk 4 , p 21 ( Cip 1 ) , and p 27 ( Kip 1 ) was determined using immunofluorescent staining . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| To elucidate the biochemical processes involved in the pathogenesis of B CLL and MCL , we analyzed the expression level of a set of genes that play central roles in apoptotic or cell proliferation pathways and of candidate genes from frequently altered genomic regions , namely ATM , BAX , BCL 2 , CCND 1 , CCND 3 , CDK 2 , CDK 4 , CDKN1A , CDKN1B , E2F1 , ETV 5 , MYC , RB 1 , SELL , TFDP 2 , TNFSF 10 , and TP 53 . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| While expression of the genes for cyclin D 1 , CDK 4 , and E2F1 increased in lung adenocarcinomas relative to normal lung , expression of p 15 ( Ink4b ) , p 16 ( Ink4a ) , p 21 ( Cip 1 ) , p 27 ( Kip 1 ) , p 57 ( Kip 2 ) , and pRb genes decreased in comparison . ^^^ Competitive RT PCR showed that the levels of cyclin D 1 and CDK 4 mRNAs were 2 and 3 fold higher , respectively , in lung adenocarcinomas than in normal lung , while the mRNAs for p 15 ( Ink4b ) , p 16 ( Ink4a ) , p 21 ( Cip 1 ) , p 27 ( Kip 1 ) , and pRb were 3 to 4 fold lower in adenocarcinomas than in normal lung , thus validating the macroarray findings . ^^^ |
|
| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The effect of exposure to 50 Hz , 1 mT magnetic fields ( MF ) on the cell cycle in general , on the DNA synthesis in S phase , and on the G 1 phase regulating proteins Cdk 4 , cyclin D 1 , p16INK4a , and p21CIP1 was investigated in human amniotic fluid cells . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Additional in vitro studies demonstrated a G 1 arrest that was preceded by depletion in cyclin D 3 , elevation of p 21 ( WAF 1 ) and p 27 ( KIP 1 ) leading to a loss in activity of G 1 cdks ( cdk 2 , cdk 4 ) , and reduction in pRb phosphorylation . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the mRNA level of several genes involved in cell cycle regulation in alveolar ( ARMS ) and embryonal rhabdomyosarcomas ( ERMS ) . p 21 ( Cip 1 ) , Cyclin D 1 , Cyclin D 2 , Cyclin D 3 , CDK 2 , and CDK 4 were evaluated by RT PCR . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| This CdK 2 mediated effect should be considered in addition to the inhibition of cyclin D CdK 4 and 6 complexes by CdKI p 21 ( Waf 1 ) . . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Overexpressed Cdk 4 in epithelial cells induces a dramatic upregulation of p 16 ( INK4a ) and milder upregulation of p 53 and p 21 ( WAF 1 ) , which become unresponsive to UV irradiation . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Conditions that increased the abundance of the D cyclins also increased the abundance of enzymatically active D cyclin cdk 4 complexes in mouse embryo fibroblasts ( MEFs ) lacking both p 27 ( Kip 1 ) and p 21 ( Cip 1 ) ( p27 / p21 ( / ) ) . ^^^ However , as determined by treatment of wild type MEFs with MG 132 , maximal accumulation of D cyclin cdk 4 complexes required p 27 ( Kip 1 ) and p 21 ( Cip 1 ) and coincided with the formation of inactive D cyclin cdk 4 p27 ( Kip 1 ) or p 21 ( Cip 1 ) complexes . p 27 ( Kip 1 ) or p 21 ( Cip 1 ) also increased the abundance of D cyclin cdk 4 complexes and reduced amounts of cdk 4 activity when ectopically expressed in p27 / p21 ( / ) MEFs . ^^^ We conclude that ( 1 ) D cyclin cdk 4 complexes are formed and become active in the absence of p 27 ( Kip 1 ) and p 21 ( Cip 1 ) and ( 2 ) p 27 ( Kip 1 ) and p 21 ( Cip 1 ) maximize the accumulation but inhibit the activity of D cyclin cdk 4 complexes . ^^^ We suggest that D cyclin cdk 4 complexes are more stable when bound to p 27 ( Kip 1 ) or p 21 ( Cip 1 ) and that formation of ternary complexes also stabilizes the D cyclins . . ^^^ P27Kip1 and p21Cip1 are not required for the formation of active D cyclin cdk 4 complexes . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| This was associated with increased protein content of cyclin D 1 and Cdk 4 and decreased activation of p 21 ( cip 1 ) and p 27 ( kip 1 ) . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Biochemical analysis of tumors showed that CDK 4 sequesters the CDK 2 inhibitors p27Kip1 and p21Cip1 , suggesting that indirect activation of CDK 2 plays an important role in tumor development . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Do p27Kip1 and p21Cip1 function as activators or inhibitors of D cyclin cdk 4 activity . ^^^ In this perspective , we summarize the results of studies addressing the effects of p27Kip1 and p21Cip1 on the assembly and activation of D cyclin cdk 4 complexes . ^^^ Emphasis is placed on our experimental findings that support a model of cell cycle control in which p27Kip1 and p21Cip1 stabilize D cyclin cdk 4 complexes but inhibit D cyclin cdk 4 activity . . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The most potent compound , actein , decreased the level of cyclin D 1 , cdk 4 and the hyperphosphorylated form of the pRb protein and increased the level of p21cip1 in MCF 7 cells , changes that may contribute to the arrest in G 1 . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In rasREF cells treated with DE for 72 h in suspension culture ( a ) , the levels of cyclin D 1 , cyclin A , p 27 ( Kip 1 ) , and hyperphosphorylated Rb were decreased , but the levels of cdk 4 , cdk 6 , cdk 2 , p 16 ( INK4a ) , and p 21 ( Cip 1 ) were not affected . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| While the amounts of the cellular cyclin dependent kinase ( Cdk ) inhibitors p 21 ( Cip 1 ) , p 27 ( Kip 1 ) , and p 16 ( INK4a ) did not change in infected cells , MHV infection in asynchronous cultures induced a clear reduction in the amounts of Cdk 4 and G ( 1 ) cyclins ( cyclins D 1 , D 2 , D 3 , and E ) in both DBT and 17Cl 1 cells and a reduction in Cdk 6 levels in 17Cl 1 cells . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Biochemical analysis of the K 5 Myc epidermis showed that CDK 4 mediates the proliferative activities of Myc by sequestering p21Cip1 and p27Kip1 and thereby indirectly activating CDK 2 kinase activity . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Biophys . 41 , 131 ] , this study aims to document the influence of 50 Hz , 1 mT magnetic fields ( MF ) , with or without initial gamma ionizing radiation ( IR ) , on the following cell proliferation relevant parameters in human amniotic fluid cells ( AFC ) : cell cycle distribution , expression of the G 1 phase regulating proteins Cdk 4 , cyclin D 1 , p21CIP1 and p16INK4a , and Cdk 4 activity . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Activated Notch inhibits mitogen induced upregulation of p21Cip1 and delays cyclin D cdk 4 mediated Rb phosphorylation . ^^^ Notch dependent repression of p21Cip1 prevents nuclear localization of cyclin D and cdk 4 . ^^^ The necessity of p21Cip1 for nuclear translocation of cyclin D cdk 4 and S phase entry in endothelial cells was demonstrated by targeted downregulation of p21Cip1 by using RNA interference . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| These cells developed a G2 / M cell cycle arrest with a concomitant decreased percentage of cells in S phase ( approximately 2 fold ) , associated with an increased expression of p 21 ( WAF 1 ) , p 27 ( KIP 1 ) , as well as cyclin B 1 and decreased levels of CDK 2 , CDK 4 , and E2F4 . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| We found that overexpression of wild type FAK promoted exit from G ( 1 ) in monolayer cultures of glioblastoma cells , enhanced the expression of cyclins D 1 and E while reducing the expression of p 27 ( Kip 1 ) and p 21 ( Waf 1 ) , and enhanced the kinase activity of the cyclin D 1 cyclin dependent kinase 4 ( cdk 4 ) complex . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| All tumor samples expressed normal sized RB 1 , cyclin D 3 , CDK 2 , CDK 4 , p 21 ( CIP 1 ) , and p 27 ( KlP 1 ) proteins , and only a single tumor showed an aberrant protein band for one of these proteins , p 21 ( CIP 1 ) . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Temporal ectopic expression of either p 21 ( Cip 1 ) or p 27 ( Kip 1 ) arrested proliferation , inhibited Cdk 2 and Cdk 4 activities , and suppressed retinoblastoma phosphorylation . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Viral K cyclin interacted with cyclin dependent kinases cdk 2 , cdk 4 , and cdk 6 and with the cyclin / cdk inhibitory proteins p21Cip1 and p27Kip1 in BC 3 cell lysates . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Smad 7 inhibits TGF beta mediated downregulation of c Myc , CDK 4 , and Cyclin D 1 , and suppresses the expression of p 21 ( Cip 1 ) . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| In parallel , we measured the kinase activities and found that CDK 2 and CDK 4 were suppressed with commensurate increased levels of CDK inhibitors , p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . ^^^ These data suggested that Epimedin C arrested the proliferation of these cells at G0 / G1 phase through inhibition of CDK 2 and CDK 4 activities via an increased induction of p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . ^^^ Taken together , the molecular mechanisms of anti tumor activity of Epimedin C may be proceeded by the combined effects of the cell cycle blockage via either the inhibition of CDK 2 and CDK 4 activities , with commensurate increase in their inhibitors , p 21 ( Cip 1 ) and p 27 ( Kip 1 ) or negatively modulates the ERK / c Fos / AP 1 signaling pathway . . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Here , we suggest that the related CDK `` inhibitors ' ' p 21 ( cip 1 ) and p 27 are differentially utilized as positive CDK 4 regulators in these mitogenic stimulations . p 21 was induced by EGF + serum , but repressed by TSH , which , as previously shown , upregulates p 27 . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Valproic acid induces growth arrest , apoptosis , and senescence in medulloblastomas by increasing histone hyperacetylation and regulating expression of p21Cip1 , CDK 4 , and CMYC . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| To analyze the cell kinetics of ulcerative colitis ( UC ) associated dysplasia , cyclin A , cyclin D 1 , cyclin E , cdk 2 , cdk 4 , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) were immunohistochemically examined , in comparison with sporadic tubular adenomas . ^^^ In tubular adenomas , cyclin A , cdk 4 , p 27 ( Kip 1 ) , and p 21 ( Waf 1 ) were all expressed in the upper parts of the crypts . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| Study of the key regulators of cell cycle progression by Western blot analysis showed that the expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 ( kip 1 ) , but not that of p 21 ( cip 1 ) , was enhanced , whereas that of c Myc , cyclin E , cyclin D 2 , and cyclin dependent kinases 2 and 4 ( CDK 2 and CDK 4 ) was decreased when these cells were treated with TZD 18 ( 10 or 20 microM ) . ^^^ |
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| Interacting proteins: P11802 and P38936 |
Pubmed |
SVM Score :0.0 |
| The expression of the p 21 ( cip 1 ) , p 15 ( INK4B ) , CDK 4 , and cyclin D 1 proteins was not altered by TGFbeta 1 , treatment , except in one cell line that displayed a slight increase in p 21 protein . ^^^ |
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