| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.51845583 |
| Gankyrin also binds to CDK 4 and the tumor suppressor RB , and accelerates phosphorylation and proteasomal degradation of RB . 0.51845583^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Gankyrin contains six ankyrin repeats and interacts with Rb , Cdk 4 , and the S 6 ATPase of the 26 S proteasome . ^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Gankyrin is an ankyrin repeat oncoprotein that interacts with CDK 4 kinase and the S 6 ATPase of the 26 S proteasome . ^^^ Overexpression and precipitation of tagged gankyrin from cultured cells detects a complex containing co transfected tagged S 6 ATPase ( or endogenous S 6 ) and endogenous cyclin D dependent kinase CDK 4 . ^^^ The proteasomal ATPases are part of the AAA ( ATPases associated with diverse cellular activities ) family , members of which are molecular chaperones ; gankyrin complexes may therefore influence CDK 4 function during oncogenesis . . ^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Novel insights into the INK 4 CDK4 / 6 Rb pathway : counter action of gankyrin against INK 4 proteins regulates the CDK 4 mediated phosphorylation of Rb . ^^^ We further demonstrated a different mechanism for gankyrin to facilitate the phosphorylation of Rb , by binding with cyclin dependent kinase 4 ( CDK 4 ) . ^^^ We then showed that binding of gankyrin to CDK 4 and the consequent counter action of p 16 function were not affected by the Rb binding peptide 176LHLACDEERN185 , indicating that the two mechanisms are independent . ^^^ While the Rb binding motif is located at the fifth ankyrin repeat , truncation mutants of gankyrin , with the first three or four ankyrin repeats remaining , are sufficient for binding to CDK 4 and for counteracting the inhibitory function of p 16 . ^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Crystallization of gankyrin , an oncoprotein that interacts with CDK 4 and the S6b ( rpt 3 ) ATPase of the 19S regulator of the 26S proteasome . ^^^ Gankyrin is an oncoprotein overexpressed in hepatocarcinoma cells that binds to the cell cycle regulator CDK 4 and the S6b ATPase subunit of the regulatory component of the proteasome . ^^^ |
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| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of gankyrin , an oncoprotein found in complexes with cyclin dependent kinase 4 , a 19 S proteasomal ATPase regulator , and the tumor suppressors Rb and p 53 . ^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Structural comparison with p 16 ( INK4A ) identified several residues of gankyrin that are potentially important for CDK 4 binding , whereas observation of the thiol proton of C 180 indicated a well structured Rb binding site in the helical region of the sixth ankyrin repeat . ^^^ |
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| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| This study was undertaken to observe the effect of signal regulatory proteinalpha 1 ( Sirpalpha 1 ) on gankyrin , cyclin D 1 , CDK 4 and Fas expression in Sk hep 1 mouse hepatoma carcinoma cell line . ^^^ The protein expressions of gankyrin , cyclin D 1 , CDK 4 and Fas in various Sk hep 1 lines were determined by Western blotting . ^^^ Sirpalpha1P4Y2 downregulation of gankyrin expression was greater than that of Sirpalpha 1 ( P < 0 . 05 ) , but no significant effect of Sirpalpha 1 and Sirpalpha1P4Y2 on CDK 4 and Fas protein expression was observed in transfected Sk hep 1 lines ( P > 0 . 05 ) . ^^^ |
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| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| Gankyrin regulates the phosphorylation of the retinoblastoma protein ( pRb ) by CDK 4 and enhances the ubiquitylation of p 53 by the RING ubiquitin ligase MDM 2 . ^^^ |
|
| Interacting proteins: O75832 and P11802 |
Pubmed |
SVM Score :0.0 |
| The expressing levels of cyclin D 1 , CDK 4 , Fas , beta catenin and gankyrin in various cell lines were determined with Western blotting . ^^^ RESULTS : Sirpalpha 1 could significantly decrease the expression of cyclin D 1 , beta catenin and gankyrin , but it could n ' t affect the expression level of CDK 4 and Fas . ^^^ |
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