| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| GH stimulated tyrosine phosphorylation of the GH receptor , signal transducer and activator of transcription 3 ( Stat 3 ) , and Stat 5 . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| To investigate the mechanism involved , we studied the effects of estrogen on GH signaling through Janus kinase ( JAK ) 2 and the signal transducers and activators of transcription ( STATs ) in HEK 293 cells stably expressing the GH receptor ( 293GHR ) , HuH 7 ( hepatoma ) and T 47D ( breast cancer ) cells . 293GHR cells were transiently transfected with an estrogen receptor alpha expression plasmid and luciferase reporters with binding elements for STAT 3 and STAT 5 or the beta casein promoter . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| In liver , these responses were associated with increased abundance of the GH receptor protein ( GHR ; P < 0 . 05 ) , whereas the abundance of intracellular mediators of GH actions ( JAK 2 , STAT 5 , or STAT 3 ) remained unaffected . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| The membrane proximal region of the cytoplasmic domain of the growth hormone receptor is involved in the activation of Stat 3 . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| By using chimeric receptors consisting of the extracellular domain of growth hormone receptor and the transmembrane and cytoplasmic domain of gp 130 with progressive C terminal truncations , we showed that the membrane proximal 133 , but not 108 , amino acids of gp 130 could generate the signals for growth arrest , macrophage differentiation , down regulation of c myc and c myb , induction of junB and IRF 1 and Stat 3 activation . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| Differential activation of Stat 3 and Stat 5 by distinct regions of the growth hormone receptor . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| A novel C terminal growth hormone receptor ( GHR ) mutation results in impaired GHR STAT 5 but normal STAT 3 signaling . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| Primary cultured fibroblasts of four patients with idiopathic short stature and severe growth delay , which displayed normal growth hormone receptor expression presented a reduced ability for activation of signal transducer and activator of transcription 3 ( STAT 3 ) . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation and Western blot analyses revealed that pp 93 and pp95 / 96 are not immunologically related with Stat 1 , Stat 3 , Stat 4 , JAK 2 , and GHR . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| Each receptor mediated a dose dependent activation of STAT 1 and STAT 3 , and for IL 3R and GHR this process was enhanced by JAK 2 . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| However , GH dependent phosphorylation of Stats 1 , 3 , and 5 was observed in cells expressing full length GHR in which tyrosines 333 and 338 are mutated to phenylalanine ( GHR 1 638Y333 , 338F ) GH , whose receptor lacks previously defined Stat 1 or Stat 3 binding sites , was found in 3T3 F442A fibroblasts and 2fTGH GHR cells to stimulate tyrosyl phosphorylation of JAK 2 to a substantially greater extent than , and JAK 1 to a similar extent as , leukemia inhibitory factor ( LIF ) and / or interferon gamma ( IFN gamma ) , ligands whose receptors contains Stat 3 and Stat 1 binding sites and activate Stat 3 and Stat 1 , respectively , better than GH . ^^^ These findings suggest that : 1 ) JAK 2 is required for GH dependent phosphorylation of Stats 1 , 3 , and 5 ; 2 ) tyrosines 333 and / or 338 are required for maximal tyrosyl phosphorylation of Stats 1 , 3 , and 5 ; 3 ) Stat 5 binds to a phosphorylated tyrosine ( s ) within amino acids 454 638 in addition to tyrosines 333 and / or 338 ; 4 ) GH stimulates tyrosyl phosphorylation of JAK 1 in addition to JAK 2 with JAK 2 having a much greater response ; 5 ) some Stat 3 and Stat 5 ( and possibly Stat 1 ) may bind to nonphosphorylated amino acids in GHR or to phosphorylated tyrosines in proteins that bind to GHR ( e . g . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| The present study investigates the importance of the GHR cytoplasmic domain in the activation of STAT 3 and STAT5b . ^^^ STAT5b and STAT 3 , when each was coexpressed in COS 7 cells with WT GHR and WT JAK 2 , were both strongly tyrosine phosphorylated in response to GH . ^^^ Coexpression of STAT 3 with GHR / JAK 2 chimeras resulted in a strong GH independent tyrosine phosphorylation of STAT 3 that was 40 % as active as that seen with WT GHR plus WT JAK 2 , whereas STAT5b was more minimally phosphorylated ( 13 % of WT GHR plus WT JAK 2 ) when coexpressed with chimeras devoid of the GHR cytoplasmic domain . ^^^ Transient coexpression of each STAT together with WT JAK 2 and GHR COOH terminal truncation mutants indicated that a GH induced STAT 3 DNA binding complex , but not a STAT5b DNA binding complex , was detectable when a GHR devoid of 85 % of the cytoplasmic domain COOH terminus ( but eliciting significant JAK 2 tyrosine phosphorylation ) was expressed . ^^^ We conclude that GH induces activation of STAT 3 and STAT5b by two different pathways : one primarily dependent on activation of JAK 2 ( STAT 3 ) and another that is additionally reliant on the presence of an intact and tyrosine phosphorylated GHR cytoplasmic domain ( STAT5b ) . . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| Our results demonstrate that ( 1 ) both GHR mRNA and GHR immunoreactivity are present in HL 60 cells ; ( 2 ) GH treatment results in an increase in the phosphorylation of the GHR associated Jak 2 and Stat 3 proteins , indicating the ability of the hormone to induce receptor activation ; and ( 3 ) activation of GHR increases the activity of Akt , a serine / threonine kinase that plays a prominent role in the regulation of cell survival . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| STAT 5 , STAT 3 , GHR , Socs 1 3 , and Cis phosphorylation and abundance were assessed by using immunoblots , EMSA , and / or real time RT PCR . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| The effect of heterodimerization was studied by comparing activation of Janus kinase 2 , signal transducer and activator of transcription ( STAT ) 1 , STAT 3 , STAT 5 , and MAPK in Chinese hamster ovary cells stably transfected with chimeric genes encoding receptors consisting of cytosolic and transmembrane parts of oGHR and oPRLR , extracellular domains of human granulocyte and macrophage colony stimulating factor ( hGM CSF ) receptor alpha or beta , and cells transfected with the two forms ( alpha or beta ) of PRLR and GHR . ^^^ |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and P40763 |
Pubmed |
SVM Score :0.0 |
| NA |
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