| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| To assess the role of PAI 1 in invasion and proliferation of hepatocellular carcinoma ( HCC ) cells , HLE cells were transfected with a vector capable of expressing an antisense PAI 1 transcript . ^^^ Morphologically , PAI 1 antisense induced a spindle shape with narrower cytoplasmic processes in HLE cells . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| The following circulating inflammatory markers thought to reflect neutrophil endothelial activation and injury including circulating neutrophil elastase soluble L , E , and P selectins and thrombomodulin and von Willebrand factor antigen were assayed from venous blood samples The PAI for healthy volunteers was median 0 . 5 ( range , 1 . 73 to 0 . 27 ) 10 10 ( 3 ) / min and for patients undergoing esophagogastrectomy 0 . 005 ( range , 1 . 53 to 2 . 28 ) 10 10 ( 3 ) / min . ^^^ In the postesophagogastrectomy group , a significant elevation in circulating levels of neutrophil elastase , soluble P and E selectin , thrombomodulin , and von Willebrand factor antigen were observed relative to the control group but only circulating plasma elastase demonstrated a significant correlation with the PAI ( r 2 = . 23 , p = . 03 ) . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| PAI 1 was also converted into its 44 , 000 Mr form and released by treatment of the substratum with human leukocyte elastase . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| When human leukocyte elastase ( HLE ) activity ( 1 . 0 microgram / ml ) was analysed in the presence of PAI 1 ( 0 . 15 . 0 micrograms / ml ) , HLE activity , measured with the low molecular weight paranitroanilide substrate L pyroglutamyl prolyl L valine p nitroanilide was increased time and dose dependently ( a plateau of stimulation was reached after 30 minutes ) with a simultaneous decrease in PAI 1 inhibitory activity . ^^^ Analysing HLE PAI 1 interaction on a molecular level using SDS PAGE , no SDS stable complex formation between HLE and PAI 1 could be observed but lower molecular weight cleavage products of PAI 1 were generated . ^^^ The stimulatory effect of PAI 1 on HLE activity was not restricted to the low molecular weight pNA substrate but was also revealed using a natural substrate assay ( bovine neck ligament elastin solubilization ) . ^^^ Therefore interaction of HLE and PAI 1 seems not to be restricted just to decrease PAI 1 activity but would simultaneously also increase HLE activity , thereby supporting enzymatic activity necessary for migration of leukocytes , dissolution of blood clots and tissue remodelling . . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| The effect of the proteolytic cleavage of plasminogen activator inhibitor type 1 ( PAI 1 ) by human neutrophil elastase ( HNE ) on fibrinolysis was investigated . ^^^ HNE cleaved active PAI 1 and produced low molecular weight forms of inactive PAI 1 , as previously reported . ^^^ Latent PAI 1 was resistant to HNE treatment . ^^^ The effects of PAI 1 cleavage by HNE on clot lysis was studied in a purified system . ^^^ It was shortened when HNE ( from 0 . 6 nmol / L to 80 nmol / L ) was added and returned to the value obtained without PAI 1 by 80 nmol / L of HNE ( 20 . 0 + / 5 . 8 minutes ) . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| The effect of the proteolytic cleavage of plasminogen activator inhibitor type 1 ( PAI 1 ) by human neutrophil elastase ( HNE ) on fibrinolysis was investigated . ^^^ HNE cleaved active recombinant prokaryotic PAI 1 ( rpPAI 1 ) resulting in the formation of low molecular weight forms of rpPAI 1 as previously reported . ^^^ The fact that the strained loop of the latent form of PAI 1 is buried inside the molecule most likely accounts for its resistance to HNE . ^^^ The effect of PAI 1 cleavage by HNE on tissue type PA ( tPA ) induced clot lysis was studied in a purified system . ^^^ In the absence of PAI 1 , however , HNE did not enhance clot lysis at all . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : To investigate the role of plasma neutrophil elastase ( elastase alpha 1 proteinase inhibitor complex ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and disseminated intravascular coagulation ( DIC ) in patients with posttraumatic acute respiratory distress syndrome ( ARDS ) and to explore the time course of the changes of these factors after trauma , we performed a prospective case control study . ^^^ Plasma neutrophil elastase , PAI 1 activity , and PAI 1 antigen concentration were measured on the day of the injury and on days 1 , 3 , and 5 after admission . ^^^ RESULTS : Neutrophil elastase , PAI 1 activity , and PAI 1 antigen concentration for the ARDS patients continued to be markedly high until the fifth day of admission , and the values on the fifth day were significantly higher than those of the other two groups . ^^^ CONCLUSION : We provide precise information on the time course of neutrophil elastase , PAI 1 , and DIC in trauma patients with ARDS and those at risk of developing this syndrome . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have now studied the specificity of binding and endocytosis by VLDLR and alpha2MR / LRP among a variety of serine proteinase / serpin complexes , including various combinations of the serine proteinases urokinase type and tissue type plasminogen activators , plasmin , thrombin , human leukocyte elastase , cathepsin G , and plasma kallikrein with the serpins PAI 1 , horse leukocyte elastase inhibitor , protein C inhibitor , C 1 inhibitor , alpha 2 antiplasmin , alpha 1 proteinase inhibitor , alpha 1 antichymotrypsin , protease nexin 1 , heparin cofactor 2 , and antithrombin 3 . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Using immunohistochemistry , we analyzed the pattern of expression of u PA , PAI 1 , and PAI 2 in non small cell lung carcinomas ( NSCLC ) and neuroendocrine ( NE ) lung tumors . u PA and PAI 1 were both detected in stromal fibroblasts and in tumor cells . ^^^ In 72 NE tumors , u PA and PAI 1 were more frequently expressed in fibroblasts in high grade NE tumors ( SCLC and large cell NE tumors ) than in low and intermediate grade tumors ( typical and atypical carcinoids ) . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Together with the cleavage and inactivation of PAI 1 by human neutrophil elastase , which was reported previously from our laboratory , such neutralization of PAI 1 activity by these serine proteases was shown to be strongly involved in the coagulation associated enhancement of fibrinolytic activity . . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Besides degrading of matrix proteins , PMN E has been shown to be able to cleave and inactivate plasminogen activator inhibitor 1 ( PAI 1 ) , the main inhibitor of uPA , and alpha 2 antiplasmin , the natural inhibitor of plasmin , thus enabling an uncontrolled matrix degradation by the fibrinolytic enzymes . ^^^ Levels of complexed PMN E have been correlated with the lengths of metastasis free survival ( MFS ) , relapse free survival , and overall survival , and a comparison was made with data previously obtained for uPA and PAI 1 . ^^^ PMN E was an independent prognostic factor for MFS even in the multivariate analysis including the traditional clinical prognostic factors and the strong established biochemical prognostic factors uPA and PAI 1 . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| To study the mechanism of PAI 1 up regulation , we examined the effect of advanced glycation end products ( AGEs ) and the product of lipid peroxidation ( 4 hydroxy 2 nonenal ( HNE ) ) , both of which are endogenously generated under carbonyl stress . ^^^ Stimulation of primary white adipocytes by either AGE or HNE resulted in the elevation of PAI 1 in culture medium and at mRNA levels . ^^^ These results suggest that cellular carbonyl stress induced by AGEs or HNE may stimulate PAI 1 synthesis in and release from adipose tissues through ROS formation . . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Notably , hearts from t PA null mice released much less NE upon EFS than their wild type ( WT ) controls ( i . e . , a 76 . 5 % decrease ; P < 0 . 01 ) , whereas hearts from plasminogen activator inhibitor 1 ( PAI 1 ) null mice released much more NE ( i . e . , a 275 % increase ; P < 0 . 05 ) . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Blood samples for platelet count , platelet response to adenosine diphosphate , plasma beta thromboglobulin , inactivated complement 3b , neutrophil elastase , fibrinopeptide A , prothrombin fragment F1 . 2 , thrombin antithrombin complex , tissue plasminogen activator , plasminogen activator inhibitor 1 , plasmin alpha 2 antiplasmin complex , and D dimer were obtained at these times : after heparin was given , 5 and 30 minutes after cardiopulmonary bypass was started , within 5 minutes after bypass was stopped , and 15 minutes after protamine was given . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| No differences between groups , however , could be observed with respect to partial thromboplastin time , prothrombin time , platelets , plasminogen activator inhibitor 1 , soluble tumor necrosis factor receptor 2 , neutrophil elastase , interleukin ( IL ) 1 receptor antagonist , IL 6 , and IL 8 . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| Platelet count , platelet aggregation , beta thromboglobulin , prothrombin fragment F1 . 2 , thrombin antithrombin complex , plasminogen activator inhibitor 1 , alpha 2 plasmin inhibitor plasmin complex , D dimer , neutrophil elastase , and interleukin 6 were measured before , during , and after bypass . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| The humoral mediators measured were the chemokine IL 8 , plasminogen activator inhibitor 1 , and neutrophil elastase ( NE ) . ^^^ Before DHP PMX treatment , the Acute Physiology and Chronic Health Evaluation 2 score was 24 + / 2 . 0 , the IL 8 level was 54 + / 15 . 8 pg / ml , plasminogen activator inhibitor 1 was 133 + / 28 . 1 ng / ml , and NE was 418 + / 72 . 1 mug / l . ^^^ The mean blood levels of plasminogen activator inhibitor 1 , NE , and IL 8 were significantly decreased from 48 hours after DHP PMX treatment . ^^^ |
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| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05121 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|