Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P18065 and P05019	Pubmed	SVM Score :1.0591576
However , Val44Met IGF 1 binds IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and IGFBP 6 with equal affinity to IGF 1 , suggesting the maintenance of overall structure , particularly in the IGFBP binding domain . 1.0591576^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.53362908
Sheep fibroblasts produced seven IGFBPs : a 36 . 5 41 kDa protein induced in vitro by IGF 1 , likely representing oIGFBP 3 ; a 28 . 5 kDa protein that reacted with antisera to human IGFBP 2 , likely representing oIGFBP 2 ; 25 and 27 kDa proteins induced in fetal fibroblasts by IGF 1 ; a 22 kDa protein that was inhibited by IGF 1 , likely representing oIGFBP 4 ; and 21 and 23 kDa proteins labelled only by IGF 2 , suggesting their similarities to IGFBP 6 . 0.53362908^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.50424938
Levels of the 150 kDa complex , a ternary complex of IGF 1 with IGFPB 3 and an acid labile subunit , the 50 kDa complex , a complex of IGF 1 with IGFBP 2 , were found to be lower in diabetic rats than in normal rats . 0.50424938^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The present study characterized the changes in concentrations of insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) in uterine luminal flushings , and the endometrial mRNA levels of IGF 1 , IGF 2 , and IGF binding protein 2 ( IGFBP 2 ) obtained from Days 0 through 18 of the bovine estrous cycle and early pregnancy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both analogues bound very poorly to bovine IGF binding protein 2 and slightly less well than IGF 1 to the type 1 receptor on rat L 6 myoblasts . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have used in situ hybridization to test whether this inductive activity correlates with the developmental expression of genes encoding insulin like growth factors 1 and 2 ( IGF 1 and 2 ) and / or proteins that may mediate IGF action ( IGF binding protein 2 and the IGF 1 receptor ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF binding protein 2 gene expression and the location of IGF 1 and IGF 2 in fetal rat lung . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The temporal patterns of endometrial expression for mRNAs encoding insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and the type 1 IGF receptor ( IGF IR ) were elucidated in cyclic and pregnant pigs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Samples were analysed for glucose , blood urea nitrogen ( BUN ) , non esterified fatty acids ( NEFA ) , creatinine , insulin , insulin like growth factor 1 ( IGF 1 ) and IGF 2 , tri iodothyronine ( T 3 ) , progesterone and IGF binding protein 2 ( IGFBP 2 ) to determine whether rbPL mediates similar metabolic effects to those of bGH . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Increased expression of IGF binding protein 2 during IGF 1 treatment of healthy adults and in patients with extrapancreatic tumor hypoglycemia . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Treatment with bST increased milk yield and concentrations of serum IGF 1 , hepatic IGF 1 mRNA , and serum IGF binding protein 3 , decreased concentration of serum IGF binding protein 2 , and did not alter abundance of mammary IGF 1 mRNA . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Six Holstein cows were used in a complete block design to examine effects of period of lactation and somatotropin ( bST ) administration on concentrations of insulin , insulin like growth factors ( IGF 1 , IGF 2 ) , and IGF binding protein 2 ( IGFBP 2 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 2 and IGF binding protein 2 mRNAs were regulated by E and P 4 distinct from IGF 1 mRNAs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Ontogeny of growth hormone ( GH ) , insulin like growth factors ( IGF 1 and IGF 2 ) and IGF binding protein 2 ( IGFBP 2 ) in genetically lean and obese swine . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
This study compared levels of the IGF 1 and IGF 2 , and of the IGF binding protein 2 ( IGFBP 2 ) and IGFBP 3 in vitreous from three groups with different degrees of retinal ischemia , as judged by the extent of neovascularization : a control group without new vessel formation , retinal neovascularization in patients with proliferative diabetic retinopathy , and massive ischemia of various causes resulting in rubeosis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 2 are increased in cyst fluids of epithelial ovarian cancer . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 mRNA in the bladder was elevated 3 fold after 3 days and 2 . 5 fold after 10 days , whereas IGF binding protein 2 mRNA was increased approximately 2 fold after 3 days and 5 fold after 10 days . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Circulating IGF 1 correlated negatively and IGF binding protein 2 ( IGFBP 2 ) positively with IGF 2 levels , suggesting that IGF 1 is displaced from IGFBPs by IGF 2 and that IGF 2 is a major regulator of IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The expression and regulation of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 2 ( IGFBP 2 ) , 3 , 4 , and 5 messages were studied in liver , kidney , spleen , thymus , heart , brain , skeletal muscle , testes , and epididymal ( white ) adipose tissue ( WAT ) from hypophysectomized rats infused with saline , recombinant human ( rh ) IGF 1 , or rhGH and compared with tissue messenger RNA ( mRNA ) levels in age matched normal rats . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy ( Hx ) and GH replacement therapy on the expression of IGF 1 , the IGF 1 receptor and IGF binding protein 2 ( IGFBP 2 ) in juvenile rats . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
From 8 40 days postinoculation ( dpi ) , spinal cord sections were used to correlate lesion location and severity with mRNA distributions of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , IGF 1 receptor ( IGFR 1 ) , glial fibrillary acidic protein ( GFAP ) , and myelin basic protein ( MBP ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The objective of the research was to determine whether endotoxin administration changes plasma concentrations of IGF 1 and IGF binding protein 2 ( IGFBP 2 ) in Angus 10 Hereford steers . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF binding protein 2 , which was the predominant IGF binding protein in serum by ligand blot and immunoblot , did not change with IGF 1 infusion . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine whether this microglial response upregulates astrocytic expression of IGF related proteins , we induced EAN and used in situ hybridization and immunocytochemistry to examine the mRNAs and peptides for glial fibrillary acidic protein ( GFAP ) , insulin like growth factor 1 ( IGF 1 ) , IGF 1 receptor ( IGFR 1 ) and IGF binding protein 2 ( IGFBP 2 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Messenger RNA and peptide distributions of insulin like growth factor 1 ( IGF 1 ) , IGF 1 receptor ( IGFR 1 ) , IGF binding protein 2 ( IGFBP 2 ) , glial fibrillary acidic protein ( GFAP ) , and myelin basic protein ( MBP ) were studied 3 56 days after CI by in situ hybridization and immunocytochemistry . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
For example , in the nephrotic syndrome , proximal tubule fluid contains IGF 1 at biologically meaningful concentrations in association with IGF binding protein 2 ( IGFBP 2 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study we examined expression of mRNA for IGF 1 and IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and IGF receptors 1 ( IGFR 1 ) and 2 ( IGFR 2 ) during fetal lung development and in early postnatal and adult lungs by the polymerase chain reaction ( PCR ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Up regulation of pituitary [ 125I ] insulin like growth factor 1 ( IGF 1 ) binding and IGF binding protein 2 and IGF 1 gene expression by estrogen . ^^^ To evaluate the effects of estrogen on the rat pituitary insulin like growth factor ( IGF ) system , binding of [ 125I ] IGF 1 and in situ hybridization for IGF 1 , the IGF 1 receptor and IGF binding protein 2 ( IGFBP 2 ) were coupled with quantitative autoradiography . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The increase in IGF 1 levels was accompanied by a significant increase in IGF binding protein 2 levels , a slight reduction in IGF binding protein 3 levels , and an increase in levels of IGF binding protein 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Corneal endothelial cells also produce IGF binding protein 2 ( IGFBP 2 ) , which appears to bind IGF 1 that has been introduced exogenously in the medium . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Patients were compared to normal men by investigating GH sampling every 10 min for 24 h and serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and GH binding protein . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both hormone infusions increased the amounts of IGF 1 in serum , IGF 1 mRNA in liver , and IGF binding protein 3 in serum and decreased IGF binding protein 2 in serum , but these effects were less for cows given releasing factor than for those given bST . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Angiotensin 2 markedly reduced plasma IGF 1 levels ( 56 and 41 % decrease at 1 and 2 wk , respectively ) and IGF binding protein 3 levels , and increased IGF binding protein 2 levels , a pattern suggestive of dietary restriction . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the nephrotic syndrome , IGF 1 is ultrafiltered into tubule fluid in association with IGF binding protein 2 and activates apical proximal tubule cell receptors . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The present studies show that in nephrotic rats , IGF 1 is ultrafiltered in conjunction with IGF binding protein 2 and is present in proximal tubular fluid at 1 . 35 nM . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 2 ( IGF BP 2 ) in the hippocampus following cytotoxic lesion of the dentate gyrus . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Cerebral contusions increase cortical expression of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 , mRNA levels increase at the contusion site ( IGF 1 , IGFBP 2 and 4 ) and along the ipsilateral cortex ( IGFBP 2 and 4 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The present study was performed to investigate the role of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 in age dependent bone loss in postmenopausal Japanese women . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 were elevated 3 to 13 fold in nondiabetic retinal ischemia and 1 . 5 to 3 fold in PDR , indicating that the changes were not restricted to diabetes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine whether peptides of the insulin like growth factor ( IGF ) system might be useful indicators of nutritional adequacy in premature infants , we studied 50 premature ( 25 34 weeks gestation ) infants prospectively to define the relationship between nutrient intake and serum concentrations of IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma concentrations of insulin and IGF 1 were also increased , but pST reduced IGF 2 and IGF binding protein 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have therefore investigated the effects of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) and intact IGFBP 3 on in vitro sperm motility . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The mechanism for increased fetal growth was examined by measuring the expression of IGF 1 and 2 and IGF binding protein 2 ( IGFBP 2 ) mRNA in liver and reproductive tissues of somatotropin and saline treated pregnant gilts . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine the effects of glucose on insulin like growth factor 1 ( IGF 1 ) induced mesangial cell ( MC ) proliferation , we have examined the relationships between IGF binding protein 2 ( IGFBP 2 ) secretion and proliferation in murine MCs ( MMCs ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Staining for IGF 1 and IGF binding protein 2 was increased ( P < 0 . 05 ) in arterioles of cholesterol fed pigs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In serum samples from diabetic rats , IGF 1 levels ( 227 + / 34 ng / mL ) were reduced as compared with control levels ( 319 + / 33 ng / mL , P = . 05 ) , and IGF binding protein 2 ( IGFBP 2 ) is increased about 2 fold . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To clarify the effects of nutritional status on bone metabolism , we examined the relationship between serum levels of nutritional indicators [ insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 ] and markers for bone metabolism [ serum osteocalcin and urinary excretion of C terminal telopeptide of collagen type 1 ( CrossLaps ) ] in 45 AN out patients , including 8 severely malnourished patients who required hospitalization and 4 hyperalimentation ( IVH ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the SF , the following parameters were determined : Interleukin 1 beta , IL 6 , IL 8 , IL 1 RA , TNF alpha , Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 as well as total proteinase activity and total collagenase activity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Addition of IGF 1 or 2 to a control pool of bovine plasma did not significantly alter control IGF binding protein 2 values in a radioimmunoassay . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To test whether hormonal regulation of IGF 1 receptor ( IGF IR ) and IGF binding protein 2 ( IGFBP 2 ) may be involved in the accumulation of IGF 1 in tanycytes , we assessed the effect of ovarian hormones on the levels of these molecules in the mediobasal hypothalamus of adult female rats . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The expression of IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 , which serve as negative modulators of IGF 1 , was enhanced in CMA . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Role of insulin like growth factor 1 ( IGF 1 ) receptor , IGF 1 , and IGF binding protein 2 in human colorectal cancers . ^^^ In this study , we assessed the potential role of insulin like growth factor 1 ( IGF 1 ) , the IGF 1 receptor ( IGF IR ) and IGF binding protein 2 ( IGFBP 2 ) in human colorectal cancer . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
EGF decreased IGF binding protein 2 ( IGFBP 2 ) and IGFBP 3 by more than 50 % in the presence of IGF 1 on PL or collagen 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 were quantified in growing Angus bulls ( n = 56 ) to determine their relationship with postweaning growth and carcass ultrasound measurements . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Ovarian follicular concentrations of activin , follistatin , inhibin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor in spontaneous menstrual cycles of normal women of advanced reproductive age . ^^^ FF was analyzed for estradiol , progesterone , testosterone , androstenedione , inhibin A and B , total activin A , total follistatin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor ( VEGF ) concentrations . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RIA and RT PCR demonstrated that the genital skin fibroblast strains expressed IGF 2 , IGF binding protein 2 , and IGF binding protein 3 , but no IGF 1 . ^^^ In conclusion , although the mechanism underlying these differences requires further study , it is conceivable that in addition to the endocrine actions of IGF 1 , IGF 2 and IGF binding protein 2 , as local growth factors , are involved in the mediation of androgen action and growth of genital tissues . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Diameter ( P < 0 . 02 ) , estradiol ( P < 0 . 001 ) , free IGF 1 ( P < 0 . 002 ) , and progesterone ( P < 0 . 003 ) were greater and IGF binding protein 2 was lower ( P < 0 . 01 ) in F 2 of the ablated group at Hour 12 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The effect of depolarization and N methyl D aspartate ( NMDA ) receptor blockade on insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 expression was analysed in vivo . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Concentrations of IGF 1 and IGF binding protein 2 ( IGFBP 2 ) in F2A changed in opposite directions at the same hours . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , IGF binding protein 2 , known to inhibit anabolic effects of IGF 1 , increased in the training subjects ( 27 . 8 + / 11 % , p < 0 . 02 ) as did IL 1beta and TNF alpha ( 51 . 5 + / 30 . 22 % , p < 0 . 02 , and 44 . 5 + / 23 . 2 % , p < 0 . 02 , respectively ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this prospective , observational study IGF 1 , IGF 2 , IGF binding protein 2 , IGF binding protein 3 , and acid labile subunit were measured by immunoassays in the wound fluid and plasma of young ( 23 . 5 + / 3 . 3 years ) and elderly ( 78 . 9 + / 6 . 2 years ) patients before and daily for 4 days after elective surgery . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study , the distribution of IGF 1 , IGF type 1 receptor ( IGF IR ) , and IGF binding protein 2 ( IGFBP 2 ) was investigated during larval and post larval developmental stages of the shi drum ( Umbrina cirrosa ) by immunohistochemistry using antisera raised against Sparus aurata IGF 1 and IGF IR , and against mouse IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGF binding protein 1 and IGF binding protein 2 , but not IGF 1 , levels of prenatally T treated females were elevated in the first month of life , a period when the prenatally T treated females were not exhibiting catch up growth . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF binding protein 2 ( IGFBP 2 ) , which binds and regulates the function of IGF 1 , is upregulated in a dose dependent manner in melanoma cells treated with IGF 1 , suggesting a possible role of IGFBP 2 in the pathogenesis of melanoma . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , acid labile subunit , and the bone and soft tissue markers , osteocalcin , carboxyl terminal propeptide of type 1 procollagen , carboxyl terminal cross linked telopeptide of type 1 collagen , and procollagen type 3 were measured . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To elucidate the potential molecular mechanisms leading to the increase in adrenal growth , we examined adrenal expression of the cell cycle regulatory proteins ( cyclin D 1 ) and cyclin kinase inhibitory proteins ( p16ink , p21Cip ) , and insulin like growth factor 2 ( IGF 2 ) , IGF binding protein 2 ( IGFBP 2 ) and IGF 1 type 1 Receptor ( IGF1R ) from fetuses infused with metyrapone or vehicle for 15 days . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Cord blood samples were analyzed for IGF 1 , IGF 2 , IGF binding protein 2 , and ghrelin . ^^^ RESULTS : The Delta birth weight was positively correlated with Delta IGF 1 ( r = 0 . 66 ; P < 0 . 0002 ) and negatively correlated with Delta IGF binding protein 2 levels ( r = 0 . 68 ; P < 0 . 001 ) but with neither Delta IGF 2 nor Delta ghrelin . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The interaction of IGF binding protein 2 ( IGFBP 2 ) with IGF 1 and 2 has been investigated in solution using nuclear magnetic resonance ( NMR ) spectroscopy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The ovarian expression of mRNAs for aromatase , IGF 1 receptor , IGF binding protein 2 , 4 and 5 , leptin and leptin receptor in cycling ewes after three days of leptin infusion . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
One of these , IGFBP 2 , demonstrates a striking spatiotemporal relationship with IGF 1 during cerebellar and retinal development . ^^^ IGF 1 mRNA is transiently expressed in large projection neurons cerebellar Purkinje and retinal ganglion cells while IGFBP 2 mRNA is selectively expressed by contiguous neuroglia Bergmann glia in the cerebellum and Mller cells and astrocytes of the nerve fiber layer in the retina . ^^^ IGF 1 and IGFBP 2 gene expression is not only neuroanatomically coordinated but also temporally synchronized , peaking together during the postnatal maturation of these structures . ^^^ This pattern of IGF system expression suggests that IGFBP 2 is closely related to IGF 1 ' s action in the developing nervous system . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Binding of [ 125I ] IGF 1 to IGFBP 3 , IGFBP 2 , and IGFBP 1 , measured by ligand blotting , was not statistically different in the 2 groups , but binding intensities for the serum 24 / 25 kDa IGFBP were approximately 2 . 5 times greater in fracture women than control women ( P less than 0 . 0005 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of IGFBP 1 , but not those of IGFBP 2 , were increased ( approximately 4 fold ) in the DXM treated fetuses , as quantified by [ 125I ] IGF 1 ligand blotting and IGFBP 2 immunoblotting . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine whether chronic hypoxemia results in alterations in endocrine function that may contribute to growth failure , we measured growth hormone ( GH ) , somatomedins ( insulin like growth factors 1 and 2 , IGF 1 and IGF 2 ) , hepatic growth hormone receptors , and circulating IGF binding proteins IGFBP 3 and IGFBP 2 in 12 newborn lambs with surgically created pulmonic stenosis and atrial septal defect , and in 10 controls . ^^^ The increase in IGF 1 was associated with a decrease in IGFBP 3 ( hypoxemic , 5 . 09 + / 1 . 25 vs . control , 11 . 2 + / 1 . 08 arbitrary absorbency units per mm ( Au . mm ) , P less than 0 . 01 ) , and increase in IGFBP 2 ( 0 . 47 + / 0 . 03 vs . 0 . 19 + / 0 . 13 Au . mm , P less than 0 . 05 ) , but no significant downregulation of hepatic GH receptors ( hypoxemic , 106 . 1 + / 20 . 1 vs . control , 147 . 3 + / 25 . 9 fmol / mg , NS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSIONS : These observations indicate 1 ) that IGF binding proteins secreted by lung tumors are encoded by at least three different genes ; 2 ) that there may be a close association between IGF 2 and IGFBP 2 gene expression , such that , where there is production of IGF 2 , IGFBP 2 is the principal BP ; and 3 ) that the IGF 2 gene is more widely expressed than the IGF 1 gene in human lung tumor cell lines . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF infusion ( IGF 1 greater than des IGF 1 ) increased the levels of circulating IGFBP 3 and IGFBP 2 , which may act to modulate the biological effectiveness of the infused peptides . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have used in situ hybridization to localize transcripts for both insulin like growth factor binding protein 2 ( IGFBP 2 ) and its ligands , the insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) , within limb buds of rat embryos 10 16 days after conception ( equivalent to stages 1 12 of mouse limb morphogenesis , Wanek et al , 1989 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Two distinct cell types were studied with regard to IGFBP 2 expression : ( 1 ) rat hepatocytes , which produce IGF 1 at a high rate and thus regulate its plasma concentration ; and ( 2 ) rat osteoblasts , which are targets of IGF 1 action . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 has a higher affinity for IGF 2 than for IGF 1 and its affinity for both forms of IGF is greater than for human IGFBP 1 . ^^^ Like IGFBP 1 the protein can enhance the DNA synthesis response of porcine aortic smooth muscle cells to IGF 1 ; however , IGFBP 2 was much less potent . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 also caused an increase in IGFBP 2 from 315 + / 136 to 675 + / 304 ng / mL ( P less than 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine if IGFBP 2 modulates IGF responses in IEC 6 cells , the IGF 1 analogs , Des ( 1 3 ) IGF 1 and [ Gln 3 , Ala 4 , Tyr 15 , Leu 16 ] IGF 1 , both of which have a reduced affinity for IGFBPs , were tested for their effects on IEC 6 cell proliferation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We studied the relationship of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , the IGF binding proteins IGFBP 1 , IGFBP 2 , and IGFBP 3 , and GH binding protein ( GHBP ; which is postulated to be derived from the extracellular portion of the GH receptor ) in normal volunteers and patients with anorexia nervosa before and after a refeeding program . ^^^ Both serum IGF 1 and IGF 2 were positively correlated with serum IGFBP 3 and negatively correlated with serum IGFBP 1 and IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In umbilical cord serum , by contrast , most IGF 1 was present in the 40 kDa binding proteins ( consisting of IGFBP 1 and IGFBP 2 ) . ^^^ In the fetal circulation IGFBP 1 and IGFBP 2 appear to be the major binding proteins for IGF 1 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and retinoic acid modulation of IGF binding proteins ( IGFBPs ) : IGFBP 2 , 3 , and 4 gene expression and protein secretion in a breast cancer cell line . ^^^ IGF 1 alone stimulated MCF 7 cell proliferation , and this was associated with a large increase in IGFBP 2 in the CM . ^^^ The effect of IGF 1 on IGFBP 2 levels and the synergistic action of IGF 1 and RA on IGFBP 3 levels in CM were blocked by alpha IR 3 , a monoclonal antibody to the human IGF 1 receptor , indicating that these effects required signal transduction through the IGF 1 receptor . ^^^ In contrast , the increase in IGFBP 2 protein in CM after IGF 1 treatment appeared to be greater than the increase in IGFBP 2 mRNA levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Ligand blotting with [ 125I ] IGF 1 revealed that serum levels of IGFBP 1 ( 32 K ) were greater in SGA than shams , while immunoblotting with specific antiserum demonstrated that levels of IGFBP 2 ( 34 K ) , the major fetal rat IGF BP , were similar in serum from SGA and shams litters . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Hypophysectomy significantly decreased IGFBP 3 , IGF 1 , and IGF 2 in nonpregnant mouse serum , increased the amount of IGFBP 2 found in pregnant mouse serum , and had no effect on IGF 1 or 2 concentration in day 14 pregnant mouse serum . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
One of these binding proteins , IGFBP 2 , is present at high levels in fetal rat plasma and binds both IGF 1 and IGF 2 with high affinity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Malnutrition caused a significant reduction in both serum IGF 1 and 2 after day 12 PP , while causing an elevation in serum IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Using RIA analysis , bovine mammary cells cultured in the presence of IGF 1 produced 20 25 ng / ml IGFBP 2 compared to control cultures which secrete approximately 1 . 0 ng / ml . ^^^ Cells exposed to des 3 IGF 1 produced 40 60 % less IGFBP 2 whereas insulin and IGF 2 did not stimulate significant IGFBP 2 production . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The results demonstrate specific changes in liver IGF 1 and IGFBP 2 gene expression and brain IGF 2 gene expression in animals that are growth retarded because of a restriction of dietary energy . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The results show that IGF 1 can induce IGFBP 3 and IGFBP 2 independently of GH and that IGF 1 is a major controller of these binding proteins . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Therefore , activation of the type 1 IGF receptor was required to elicit increases in cell number and IGFBP synthesis and secretion , and the actions of IGF 1 and 2 were likely blocked by binding to the large amounts of IGFBP 2 that were secreted . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The two BPs isolated from serum , which correspond to the 32 30K CSF BP and IGFBP 2 , had affinities for IGF 2 and IGF 1 similar to those of the CSF BPs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These studies were undertaken to determine which forms of IGFBP are secreted by endometrial carcinoma ( HEC 1B ) and breast carcinoma ( MDA 231 ) cells , to characterize variables that control IGFBP secretion , and to study the effect of IGFBP 1 and IGFBP 2 on IGF 1 stimulated cell proliferation . ^^^ IGF 1 stimulated the secretion of IGFBP 3 from both cell types and IGFBP 2 from HEC 1B cells but either decreased or caused no change in secretion of IGFBP 1 and a 24 , 000 Mr form . ^^^ Bovine IGFBP 2 did not potentiate IGF 1 stimulated cell proliferation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 2 ( IGFBP 2 ) is one of a family of IGFBPs that are present in extracellular fluids , and binds both IGF 2 and IGF 1 with high affinity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The fetal expression pattern of high IGF 2 and IGFBP 2 is replaced by the adult pattern of low levels of IGF 2 and IGFBP 2 and high levels of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To test the hypothesis that growth hormone ( GH ) insensitivity is responsible , amongst other mechanisms , for impaired growth in uraemic children , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 were measured by radioimmunoassay in normal control children , in patients with end stage renal failure ( n = 51 ) and in patients with preterminal chronic renal failure ( n = 11 ) and the production rate of IGF was calculated . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We examined regulation of the genes for IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 2 in fetal rat liver in an experimental model for intrauterine growth retardation caused by maternal fasting on days 17 21 of gestation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The addition of increasing concentrations of unlabeled IGF 1 in the presence of each IGFBP showed that either IGFBP 1 or IGFBP 3 , but not IGFBP 2 , resulted in a paradoxical increase in [ 125I ] IGF 1 binding to the cell surface . ^^^ In a physiological salt solution , the order of affinity of the IGFBPs for IGF 1 was IGFBP 3 greater than IGFBP 1 greater than IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CHP 100 , a PNET cell line with the t ( 11 ; 22 ) translocation , was shown to secrete both IGF 1 protein and an IGF binding protein , IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Chinese hamster ovary ( CHO ) cells stably transformed with the IGFBP 2 cDNA secreted a 36 kd protein which bound , with different affinities , IGFII and IGFI , but did not bind insulin . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum from Zn deficient animals labeled in vitro with [ 125I ] IGF 1 displayed three peaks of tracer activity : 150 kd ( IGFBP 3 ) , 37 kd ( IGFBP 2 and 1 ) , and 8 kd ( free [ 125I ] IGF 1 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , the fetal mRNA expression patterns of IGF 1 , IGF 2 and IGFBP 2 , 3 , 4 , 5 and 6 were not grossly altered by maternal diabetes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Characterization of the IGF system [ IGF 1 , IGF 2 , type 1 IGF receptor , and six IGF binding proteins ( IGFBPs ) 1 6 ] revealed that IEC 6 cells express high levels of type 1 IGF receptor mRNA , low or undetectable levels of IGF 1 and IGF 2 mRNAs , and mRNA for only one of the six IGFBPs , IGFBP 2 . ^^^ EGF and IGF 1 both reduce IGFBP 2 mRNA expression , and inhibitory effects of EGF and IGF 1 in combination are additive . ^^^ EGF reduces IGFBP 2 accumulated in conditioned medium relative to levels observed with IGF 1 alone . ^^^ These effects of EGF on type 1 IGF receptor expression and on levels of IGFBP 2 mRNA and IGFBP 2 in medium may contribute to synergistic mitogenic effects with IGF 1 by promoting IGF 1 responsiveness . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To determine the developmental expression and localization of mRNA for insulin like growth factor binding protein 2 ( IGFBP 2 ) , a major binding protein of IGF 1 and IGF 2 , in ocular tissues of the embryonic and early posthatched chick . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Previous studies have shown that IGFBP 2 gene expression is localized in astroglia , where it is anatomically and temporally coordinated with neuronal IGF 1 expression during postnatal brain development . ^^^ The present study shows that IGFBP 5 gene expression is also highly abundant during brain development and also demonstrates significant spatiotemporal correlation with IGF 1 , but exhibits a neuroanatomical distribution that is entirely distinct from IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In addition , EGF inhibited IGFBP 2 secretion and IGF 1 tended to increase it . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We therefore investigated the effect of recombinant IGFBP 3 as well as IGFBP 2 , 4 and 5 on IGF 1 stimulation of DNA synthesis and IGF 1 binding in the MCF 7 human breast carcinoma cell line . ^^^ IGFBP 2 and 3 enhanced IGF 1 stimulation of DNA synthesis in MCF 7 cells while IGFBP 4 and 5 had no effect . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGFBP 2 seem to be particularly sensitive to dietary protein alterations . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Like serum IGFBP 2 , levels of immunoreactive IGF 1 in serum are higher in the second week of embryogenesis than the first . ^^^ Despite this correlation , changes in IGFBP 2 do not appear to be regulated by IGF 1 : ( a ) serum IGF 1 decreases after day 15 , whereas IGFBP 2 levels remain stable until hatching ; ( b ) vitreous IGF 1 , like serum IGF 1 , is higher in the second week of embryogenesis , whereas vitreous IGFBP 2 is highest in the first week ; ( c ) embryos cultured ex ovo express IGFBP 2 at E 15 E19 , although they lack the normal mid embryogenesis surge in IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
By contrast , IGF 1 correlated with the reciprocal of both IGFBP 1 and IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Islets enriched in beta cells following a 5 day preincubation regime were maintained in various concentrations of glucose ( 1 . 4 16 . 7 mmol / l ) or amino acids ( x 1 x 3 total concentrations ) , with or without exogenous IGF 1 , 2 , IGFBP 1 or IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factors 1 ( IGF 1 ) and 2 ( IGF 2 ) and their binding proteins ( IGFBP 1 , IGFBP 2 and IGFBP 3 ) were measured by radioimmunoassay in plasma and milk samples collected throughout the study . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Ligand blotting with IGF 1 demonstrated a 31 kDa protein in both membranes , identified by immunostaining as IGF binding protein 5 ( IGFBP 5 ) , not IGFBP 1 or IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The genes for IGF 1 and IGF 2 , IGFBP 2 , insulin receptor , and type 1 and 2 IGF receptors were detected . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These results indicate that the production of IGF 1 and the expression of IGF 1 receptors and IGFBP 2 are up regulated by the activation of adenylate cyclase and protein kinase C , respectively , in osteoblastic MC3T3 E 1 cells . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , mean IGFBP 2 concentrations rose by 40 % ( P < 0 . 05 ) after IGF 1 and oral glucose , but there was no change in response to oral glucose alone . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The specific effect of testosterone , but not IGF 1 , was to increase serum IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The cell lines expressed luteinizing hormone receptor ( LH R ) ( a Leydig cell specific gene ) , insulin like growth factor 1 , insulin like growth factor 1 receptor ( IGF 1 R ) and insulin like growth factor binding protein 2 ( IGFBP 2 ) genes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Before treatment ( 0 h ) , GRFi cows had lower serum ST ( 0 . 6 + / 0 . 2 vs . 2 . 2 + / 0 . 2 ng / ml ; p < 0 . 01 ) and IGF 1 ( 26 + / 4 vs . 72 + / 4 ng / ml ; p < 0 . 01 ) , but greater IGFBP 2 ( 594 + / 48 vs . 384 + / 52 ng / ml ; p < 0 . 01 ) than HSAi cows . ^^^ Serum and FFL concentrations of IGF 1 or IGFBP 2 were not different between rbST and rbST eCG treated cows or between VEH and eCG treated cows at Hour 40 after the initial treatment injection ; therefore , data were combined and designated as rbST and VEH , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We report ( 1 ) an increase in serum IGF 2 concentrations ( about 2 fold ) , whereas IGF 1 concentrations are regarded as normal when aging is taken into account ; ( 2 ) an apparent increase in serum IGFBP 3 levels when compared to those of healthy elderly subjects , IGFBP 3 only being detected in the 150 kDa IGFBP ternary complex as in normal serum ; ( 3 ) abnormally elevated serum IGFBP 2 levels taking into account the apparent concentrations of IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Treatment of the cells with various concentrations of IGF 2 affects only the level of expression of type 2 IGF receptor , whereas IGF 1 and insulin appear to influence only the expression of IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGF 2 had no effect on IGFBP 2 steady state transcript levels but enhanced the level of IGFBP 5 transcripts ( approximately fourfold ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Our results showed that IGF 1 , IGF 2 , IGFBP 2 and 3 mRNAs were expressed in uterine fibroids , and that IGFBP 1 mRNA or protein was not detected in fibroids . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
During food restriction , plasma IGF binding protein 3 ( IGFBP 3 ) and to a lesser extent IGFBP 1 were decreased , and IGFBP 2 was increased ; after switching to ad libitum feeding , plasma levels of IGFBP 2 normalized , but levels of IGFBP 1 and IGFBP 3 remained low in the face of normalized plasma IGF 1 levels . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In all subjects a single blood sample was drawn for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and GH measurements by specific RIAs . ^^^ As IGF 2 is normal and IGFBP 1 and IGFBP 2 are inversely correlated to the serum GH levels in the acromegalic patients , we speculate that the molar ratio between IGF 1 and IGFBP 3 reflects free ( biologically active ) IGF 1 and is dependent on GH levels . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Exposure of T47D cells to IGF 1 resulted in the appearance of IGFBP 2 , 4 , and 5 in conditioned medium but had no effect on the levels of IGFBPs in Triton 10 100 extracted cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Use of insulin like growth factor binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and IGF 1 for assessing growth hormone status in short children . ^^^ Insulin like growth factor binding protein 2 and 3 ( IGFBP 2 and 3 ) are members of a family of proteins that are present in extracellular fluids and bind IGF 1 and 2 . ^^^ IGFBP 2 is regulated differently from IGF 1 and IGFBP 3 , because its serum concentrations are elevated in some adults with GH deficiency ( GHD ) , whereas IGF 1 and IGFBP 3 concentrations are usually decreased . ^^^ The purposes of this study were to define the normal range of IGFBP 2 concentrations in children , to determine its efficacy in the diagnosis of GHD , and to compare the diagnostic value of measurements of the serum GH response to provocative testing with results of measurements of IGFBP 2 , IGFBP 3 , and IGF 1 . ^^^ Thirty nine of 49 children with GHD and low IGF 1 values ( serum GH response , < or = 1 ng / mL after 2 provocative tests ) had serum IGFBP 2 concentrations that were greater than 2 SD above their corresponding age adjusted means . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Conversely , parental MAC T cells expressed little IGF 1 and secreted primarily IGFBP 2 ( 29 32 kDa ) in response to stimulation with INS , dexamethasone ( DEX ) , or IGF 1 analogs . ^^^ Moreover , inclusion of exogenous IGF 1 with CM from MD IGF 1 cells triggered an additional 3 . 0 fold increase in label incorporation , but only a 1 . 6 fold increase in the presence of IGFBP 2 containing media conditions by MAC T cells . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Fasting for 2 days reduced IGF 1 mRNA level and increased IGFBP 2 mRNA level in the SDR , as well as in the normal rat , suggesting the presence of factors other than reduced serum GH responsible for fasting induced alteration in the expression of these mRNAs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGFBP 2 and 3 mRNAs and synthesized peptides , detected by in situ hybridization and immunohistochemistry respectively , were found to co localize predominantly in follicular epithelial cells . ^^^ The results suggest that an increased expression of IGF 1 may contribute to early goitre formation , but that a relative increase in the abundance of IGFBP 2 and 3 may limit IGF availability at later times , and facilitate a slowing of thyroid growth rate . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have recently suggested that the bioavailability of circulating IGF 1 is increased in the human fetus due to the molar excess of IGF 1 plus IGF 2 relative to IGFBP 3 as well as the increased concentrations of IGFBP 2 , which does not form a long lived ternary complex . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Primary preadipocyte cultures expressed IGF 1 , IGFBP 2 , and IGFBP 4 messenger RNAs ( mRNAs ) , and conditioned medium ( CM ) contained IGFBP 3 [ approximately 46 , 000 mol wt ( M ( r ) ) ] , IGFBP 4 ( 24 , 000 M ( r ) ) , and a 30 , 000 M ( r ) IGFBP identified by immunoblot as IGFBP 2 . ^^^ Like primary cultures , undifferentiated 3T3 L 1 cells expressed IGFBP 4 mRNA , but insignificant levels of IGF 1 and IGFBP 2 mRNAs . 3T3 L 1 cell CM contained IGFBP 3 and IGFBP 4 , and with the addition of IGF 1 , a 30 , 000 M ( r ) IGFBP was also present . ^^^ The presence of a larger isoform of IGFBP 2 in a differentiation dependent manner and a potentially novel IGFBP in response to IGF 1 suggests that these IGFBPs may be important in modulating IGF 1 action in adipogenesis . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
When IGF 1 binding protein ( IGFBP ) mRNA levels were studied under these conditions , we found that IGFBP 1 mRNA was not detected in spleen ; IGFBP 2 mRNA levels were reduced in Hx rats ( 67 . 9 + / 7 . 4 % of control values , P < 0 . 05 ) and bGH treatment prevented this reduction ( 95 . 5 + / 12 . 2 % , NS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Thus , IGFBP 1 and , to a lesser extent IGFBP 2 , cause inhibition of IGF 1 action by sera from fasted and scorbutic guinea pigs and may inhibit collagen gene expression in vivo . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Basic FGF enhanced IGFBP 2 mRNA whether alone or in combination with IGF 1 and enhanced only IGFBP 4 mRNA when given alone . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
High concentrations of IGF 1 were measurable in interstitial fluid by radioimmunoassay , and IGF 1 binding proteins ( IGFBPs ) were readily detectable in interstitial fluid by ligand blotting , the predominant type being IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have examined the regulation of IGFBP 2 expression by IGF 1 and insulin in 293 cells , a cell line derived from human embryonic kidney . ^^^ IGFBP 2 levels were increased 6 to 7 fold following incubation with IGF 1 , IGF 2 or insulin for 48 h . ^^^ The stimulation of IGFBP 2 by IGF 1 and insulin was reversibly abolished by incubation with protein synthesis inhibitors such as cycloheximide . ^^^ Biosynthetic labeling of quiescent 293 cells using [ 35S ] cysteine indicated that incubation with insulin or IGF 1 for 24 h increased the synthesis of total cell proteins ( predominantly intracellular ) and IGFBP 2 ( predominantly secreted ) to a similar extent ( 2 to 4 fold ) . ^^^ These results suggest that the increase in IGFBP 2 secreted by 293 cells after incubation with IGF 1 or insulin largely results from a general stimulation of protein synthesis . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Circulating concentrations of IGF 1 decreased nearly 50 % , IGF 2 decreased 28 % and IGFBP 2 increased 49 % during the 2 d of feed deprivation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
After rhIGF 1 treatment , plasma IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 2 all increased significantly , whereas IGFBP 3 did not change . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have studied its ontogenesis and measured serum concentrations of ovine IGFBP 2 after bovine growth hormone ( bGH ) , ovine placental lactogen ( oPL ) and IGF 1 treatment . ^^^ In well fed yearling lambs , treatment with IGF 1 reduced IGFBP 2 values by 27 % ( P < 0 . 05 ) relative to control animals . ^^^ The changes in plasma levels of IGFBP 2 were positively related to changes in IGF 2 while there was a negative relationship between circulating IGF 1 and IGFBP 2 such that both IGF 1 and IGF 2 may play a role in the regulation of IGFBP 2 in serum . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Porcine aortic smooth muscle cells secrete two forms of insulin like growth factor ( IGF ) binding proteins ( IGFBP 2 and 4 ) , and both forms have been shown to modulate IGF 1 actions in this cell type . ^^^ The protease cleaved IGFBP 2 into 25 and 16 kD fragments , which had reduced IGF 1 binding activity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 expression did not vary with IGF 1 or dexamethasone treatment in these cells ; IGFBP 1 mRNA was not detectable , and IGFBP 6 mRNA was low and inconsistent . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , liver IGFBP 2 mRNA was much lower in amount than IGF 1 mRNA and IGFBP 4 mRNA and was sharply elevated by fasting , and decreased by refeeding . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Removal of the obstruction 10 days after ligation caused a regression of bladder wet weight , and resulted in normalization of IGF 1 , IGFBP 2 and 4 mRNA . ^^^ The results show a close correlation between progression or regression of smooth muscle hypertrophy and differential regulation of IGF 1 , IGFBP 2 and 4 mRNA , indicating that the IGF system may play a role in smooth muscle hypertrophy . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In control cultures , MC3T3 E 1 cells expressed the mRNAs for IGF 1 , IGF 2 , and IGFBP 2 , 4 , 5 , and 6 but not IGFBP 1 or 3 . ^^^ A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , FGF , TGF beta and EGF failed to increase IGFBP 2 using either experimental paradigm . ^^^ Increased abundance of these fragments was also observed following IGF 1 exposure , although corresponding decreases in intact IGFBP 2 were not usually observed . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : IGF 1 , IGF 2 , the type 1 receptor , the insulin receptor , and IGFBP 2 6 are constitutively expressed in porcine myocardium . ^^^ The expression of IGF 1 , the receptors , and IGFBP 2 , 3 , 4 , and 6 remained unchanged during the experimental protocol . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Anti IGFBP 2 antibodies partially blocked the inhibitory role in IEC 6 cell growth evoked by des ( 1 3 ) IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum levels of insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) in healthy infants , children , and adolescents : the relation to IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , age , sex , body mass index , and pubertal maturation . ^^^ Therefore , we measured IGFBP 3 , IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 2 levels by RIA in 907 healthy children to establish well characterized normative data on IGFBP 3 according to age , sex , and pubertal stage and to study the complex relationship between IGFs and their BPs in puberty . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In Nagase analbuminemic rats , the IGF 1 levels and IGFBP distribution in serum are normal suggesting that the reduced albumin levels in the nephrotic syndrome do not cause the increased liver synthesis and serum levels of IGFBP 2 . ^^^ Because the 150K ternary complex in serum but not the smaller binding protein complex is restricted to the intravascular space , the shift of binding from IGFBP 3 ( ternary complex ) to IGFBP 2 ( binary complex ) in nephrotic rat serum may help to maintain tissue availability despite the reduction in serum IGF 1 levels . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 , 3 , and 4 are expressed under basal conditions whereas IGFBP 5 is only detectable after IGF 1 induction . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the treated diabetics IGF 1 and IGF 2 were reduced ; IGFBP 2 ( only in prepubertal subjects ) and IGFBP 3 were increased . ^^^ Regression analysis revealed a negative correlation between hemoglobin ( Hb ) A1c and standard deviation scores ( SDS ) of IGF 1 and a positive association between HbA1c and IGFBP 1 SDS or IGFBP 2 SDS . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The addition of IGFBP 1 caused a 19 21 % inhibition of IGF 1 or IGF 2 stimulated migration , whereas the addition of IGFBP 2 inhibited the effect of both by greater than 60 % . ^^^ An IGF analog that has a reduced affinity for IGFBP 2 stimulated migration equally well as IGF 1 alone even in the presence of IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The effects were declined at 24 h compared with 16 h for IGFBP 2 and 3 but not for IGF 1 and 2 . ^^^ Coincubation of the cells with IGF 1 and 2 and IGFBP 2 and 3 neutralized the effects of the factors alone . ^^^ In conclusion , these results show that IGF 1 and 2 on the one hand and IGFBP 2 and 3 on the other hand exert opposite actions on [ 125I ] hGH binding , IGFBP 2 and 3 exerting probably an IGF independent effect . ^^^ Lastly , IGFBP 2 and IGFBP 3 are mitogenic for UMR 106 . 01 rat osteosarcoma cells , inducing an increase in cell number to 125 % and 142 % of control cell counts after 48 h of incubation with 1000 ng / ml IGFBP 2 and 3 , whereas IGF 1 , IGF 2 and Long R 3 IGF 1 did not stimulate proliferation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study , we describe the mRNA expression of IGF 1 , IGF 2 , type 1 IGF receptor , IGFBP 2 , IGFBP 4 and IGFBP 5 during mouse lung development as studied by in situ hybridization techniques . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Messenger RNA for IGFBP 2 , 3 , and 5 were present , while IGF 1 and IGFBP 4 mRNAs were not detected . ^^^ IGFBP 2 and 3 expression was increased in a dose dependent manner by treatment with IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 94 children with chronic renal failure ( CRF ) . ^^^ Forward stepwise regression analysis revealed that height in CRF is correlated with IGF 1 and inversely correlated with IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSIONS : IGFBP 2 has no additional discriminatory value over IGF 1 measurements for the assessment of clinical activity in acromegaly . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These relationships are consistent with circulating IGF 1 influencing IGFBPs 3 and 4 , and similarly , IGF 2 determining IGFBP 2 , or vice versa . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have previously reported the presence of IGF 1 and IGF binding proteins ( IGFBP 2 , 3 and 4 ) in rat milk . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Finally , exogenous administration of IGFBP 2 was found to inhibit the promitogenic effect of IGF 1 in a dose dependent manner . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGFBP 2 was elevated and IGF 1 was reduced in the nondiabetic Ax rats compared with sham Ax controls , but plasma IGFBP 3 and 4 were not significantly changed . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , type 1 IGF receptor ( IGFR 1 ) , IGFBP 2 and IGFBP 5 mRNA are localized in distinct cell populations , and all are expressed at the highest levels at the peak of Purkinje cell growth , active synaptogenesis and dendritic formation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 production was also inhibited by IGF 2 and [ Leu 27 ] IGF 2 ( ED 50 for both 3 ng / mL ) but not by IGF 1 at up to 30 ng / mL . ^^^ In addition to IGFBP 2 , a 35 45 kDa IGFBP identified by immunoprecipitation as IGFBP 3 was variably present on ligand blots of control conditioned medium and was consistently found in medium from cells treated with IGF 1 or IGF 2 ( ED 50 3 ng / mL ) but not with insulin at 2 mg / mL . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The prolonged high expression of serum IGFBP 2 and liver IGFBP 2 messenger RNA ( mRNA ) during the perinatal period in hypothyroid rat pups could not be normalized by GH therapy , although serum IGF 1 values ( reduced to 54 % of control levels in the hypothyroid animals ) were brought up to control level . ^^^ We conclude that GH treatment of hypothyroid animals normalized serum IGF 1 levels in the hypothyroid rat pup , but did not correct their prolonged IGFBP 2 expression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
No overall differences were found between lit / lit and lit / + mice in the binding of 125I labelled IGF 1 to IGFBP 2 , 1 or 4 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
A rise in serum levels of 34 , 30 and 24 kDa BPs ( presumably IGFBP 2 , 1 and 4 , respectively was also noted with chronic IGF 1 therapy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 had a lesser effect , while IGFBP 2 had no effect on IGF 1 plus hCG induced testosterone formation . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
GH deficiency , fasting , IGF 1 administration to patients with GH receptor deficiency , hepatic failure and insulinomas caused a moderate increase of serum IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 treatment increases serum IGFBP 2 concentration . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased the levels of IGFBP 2 and IGFBP 5 in conditioned media by greater than tenfold but had no effect on IGFBP 2 and IGFBP 5 mRNA levels , again suggesting the involvement of posttranscriptional controls . ^^^ Pretreatment of MDA MB 468 and MDA MB 231 cells with IGF 1 receptor antibody ( alpha IR 3 ) blocked the IGF 1 effect on IGFBP 3 levels in the media in both cell lines and IGFBP 2 and IGFBP 5 secreted levels in MDA MB 468 cell conditioned media . ^^^ The addition of RA also blocked IGF 1 stimulation of IGFBP 2 and IGFBP 5 levels . ^^^ Cycloheximide treatment completely blocked the RA and / or IGF 1 mediated modulation of these binding proteins , suggesting that these agents enhance IGFBP 3 , IGFBP 2 , and IGFBP 5 synthesis and consequent secretion . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Although increased , the serum IGFBP 2 concentration was inversely correlated to the IGF 1 concentration ( r = 0 . 67 , p < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We conclude that IGF 1 ( 40 micrograms / kg every 12 h ) given sc to adults with GHRD is safe ; achieves normal levels of IGF 1 ; reduces insulin , IGF 2 , and GH levels ; and increases IGFBP 2 concentrations and urinary excretion of calcium . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Nocturnal levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 remained stable during both studies . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the present study , we investigated IGFBP 2 effects in lung tumor cells by examining the influence of IGFBP 2 on IGF receptor interaction and the biological actions of IGF 1 and IGF 2 . ^^^ In NSCLC cells , both IGF 1 and IGF 2 bound predominantly to IGF 1 receptors , whereas in SCLC cells binding was principally to surface associated IGFBP 2 . ^^^ Soluble IGFBP 2 inhibited the binding of radiolabeled IGF 1 and 2 to both SCLC and NSCLC cells in a concentration dependent manner and inhibited IGF stimulated DNA synthesis in NSCLC cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
After 24 h of treatment , IGF 1 , IGF 2 , and high concentrations of insulin increased IGFBP 2 and IGFBP 3 levels . ^^^ Time course studies demonstrated that IGF 1 rapidly induced the mRNA levels of both IGFBP 2 and IGFBP 3 within 7 . 5 12 h . ^^^ IGFBP 2 mRNA levels showed a biphasic response to IGF 1 , a rapid increase at 7 . 5 h , followed by a decline at 12 18 h , and then another increase at 24 h . ^^^ The stability of IGFBP 2 mRNA at 24 h of IGF 1 treatment was not significantly altered from that under control conditions , indicating that the changes in IGFBP 2 mRNA levels were not due to alterations in the stability of the mRNA . ^^^ In situ hybridization studies demonstrated an increase in the abundance per cell of IGFBP 2 and IGFBP 3 mRNAs in either one or both types of astroglial cells after IGF 1 or IGF 2 treatment . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Northern blots of total cellular RNA were probed with oligonucleotides for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and a human IGFBP 1 cDNA . ^^^ The data showed that in fibroids compared to myometrium , 1 ) the relative abundance of IGF 1 mRNA was not different , but there was an increase in the relative abundance of IGF 2 mRNA ( P < 0 . 001 ) ; 2 ) IGFBP 1 mRNA was undetectable in fibroids and detectable in only 1 specimen of myometrium ; 3 ) there was no difference in the relative abundance of IGFBP 2 mRNA , but there was an increase in the relative abundance of IGFBP 3 mRNA in myometrium ( P < 0 . 05 ) . ^^^ Our data show that the mRNAs encoding IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 are expressed in both fibroids and myometrium and that fibroids express more IGF 2 and less IGFBP 3 mRNA than myometrium . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 mRNA and IGFBP 5 mRNA are localized in both adipocytes and stromal vascular cells , whereas IGFBP 2 and 3 expression is restricted to stromal vascular tissue . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the mechanism of follicular arrest take part increased level of binding proteins for IGF 1 , mainly IGFBP 2 , 4 and 5 inhibit FSH and IGF 1 action . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGFBP 2 , 3 , and 4 mRNAs are detected in surrounding mesenchymal tissue , but are not detected in mesenchymal condensations or chondrocytes at any stage of development . ^^^ IGF 1 , IGF 2 , and IGFBP 2 , 4 , 5 , and 6 mRNAs are detected in osteoblasts localized in zones of endochondral ossification from E 18 to at least P 25 . ^^^ The present study demonstrates , for the first time , the cell specific patterns of IGF 1 and 2 receptor and IGFBP 2 to 6 gene expression during the processes of chondro and osteogenesis in vivo . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
When the cells were incubated with IGF 1 , IGFBP 3 and 4 concentrations increased , but IGFBP 2 production was much less stimulated . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
It has previously been demonstrated that 17 parents heterozygous for the Ecuadorean mutation of the GH receptor differed little in biochemical measures ( GHBP , IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 ) from Ecuadorean controls . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Relative mRNA levels of IGF 1 , IGFR , IGFBP 2 , and GFAP in the ipsilateral facial nucleus were highest 4 7 days after transection and declined thereafter . ^^^ Double immunostaining experiments showed that both IGF 1 and IGFBP 2 were localized in GFAP positive astrocytic processes , many of which were perineuronal . ^^^ Our findings suggest that astrocytic production of IGF 1 and IGFBP 2 may accompany regeneration of neurons undergoing retrograde changes induced by axotomy . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGFBP 2 mRNAs increased and decreased , respectively , with follicle development until day 7 when IGF 1 expression declined . ^^^ Regression analysis of IGF 1 and IGFBP 2 mRNA expression was performed to assess the relative strength of correlations with day , diameter and steroid concentrations as covariates . ^^^ In conclusion , the expression of IGF 1 and IGFBP 2 mRNAs in the ovarian follicle are discordantly regulated during follicular growth and maturation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Secretion of IGFBP 2 was not regulated to a significant degree , with insulin , IGF 2 , IGF 1 , forskolin , and dibutyryl cyclic adenosine monophosphate ( cAMP ) inducing minimal changes in IGFBP 2 secretion of less than 30 % by radioimmunoassay ( RIA ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
A slight but significant increase in IGFBP 3 was observed coincident with the IGF 1 peak , whereas IGFBP 2 increased after a delay of about 10 hours . ^^^ In the patients with GHIS , chronic IGF 1 treatment , with twice daily injections for 6 months , caused a significant steady decline of IGF 2 and an increase in IGFBP 2 , but had no effect on IGFBP 1 and IGFBP 3 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , IGF 1 and IGFBP 3 are reduced , and IGFBP 1 and IGFBP 2 are increased in children with ESLD . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
At 72 h of recovery , although IGFBP 2 and type 1 IGF receptor mRNA levels remain suppressed , gene expression of both IGF 1 and IGFBP 5 was activated in reactive astrocytes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the ovine fetus , insulin like growth factor 1 ( IGF 1 ) is bound mainly to the low molecular weight insulin like growth factor binding proteins ( IGFBP ) , IGFBP 1 and IGFBP 2 , with little binding to IGFBP 3 until near term at 147 days gestation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
While mRNA transcripts for IGF 1 , GH receptor , and IGFBP 2 , 3 , 4 , and 5 were readily detectable in ovarian tissue , GRFi had no effect on ovarian levels of mRNA for each of these proteins . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Expression of mRNA for IGF 1 , IGFBP 2 , 3 , 4 , and 6 was present in endometrial specimens . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Previous studies have shown that porcine aortic smooth muscle cells ( SMCs ) secrete two insulin like growth factor binding proteins ( IGFBP ) , IGFBP 2 and 4 , and that these IGFBPs modulate IGF 1 stimulated SMC proliferation and migration . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The different affinities of des ( 1 3 ) IGF 1 for IGFBP 3 , 2 , and 1 lead us to suggest that IGFBP 2 and / or 1 are present in the deep stroma and glands , whereas IGFBP 3 may be responsible for the additional binding sites in the myometrium and blood vessel walls . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Human seminal plasma contains IGF 1 and 2 , IGFBP 2 and 4 , as well as IGFBP 3 fragments and IGFBP 3 protease activity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
This increase could not be accounted for by paracrine or autocrine factors , or by the addition of insulin , IGF 1 , transforming growth factor beta ( TGF beta ) , cAMP or IGFBP 2 to the culture medium . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Immunocytochemistry , in situ hybridization , and radioimmunoassay were employed to examine the cellular distribution of mRNAs and proteins for IGF 1 , 2 , IGF II / M6P receptor , IGFBP 2 as well as the levels of IGF 1 and 2 in normal and unilaterally nephrectomized ( Nx ) adult rat kidneys . ^^^ In addition , immunostainable IGF 1 and IGF II / M6P receptor were noted in some inner medullary loops of Henle , while IGFBP 2 was seen in the collecting ducts and loops of Henle of the inner medullar and the renal vasculature of all animals . ^^^ These data suggest that renal IGF 1 and IGFBP 2 are synthesized at upstream sites along the nephron and then transported downstream for interaction with IGF receptors . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Using in situ hybridization , we have demonstrated mRNAs for IGF 1 , IGF IR , and IGFBP 2 to IGFBP 6 in specific histologic layers of the retina , choroid , ciliary body , and cornea in the rat . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study , we examined the effect of fetal exposure to alcohol IGF 1 and on IGF 2 and IGFBP 1 and IGFBP 2 mRNA in placenta and fetal lung tissue . ^^^ At G 20 , dams were killed , and we examined placenta and fetal lung for the steady state levels of mRNA for IGF 1 and IGF 2 and for IGFBP 1 and IGFBP 2 . ^^^ Multiple transcripts for IGF 1 and IGF 2 , 1 . 8 kb species for IG FBP 1 and 1 . 6 kb species for IGFBP 2 were detected from total RNA isolated from the fetal lung and placenta , Slot blot hydridization analysis of fetal lung RNA showed that IGF 1 mRNA was significantly increased ( p < 0 . 001 ) in AF males and females by 4 . 0 + / 0 . 28 fold and by 3 . 25 + / 0 . 2 fold , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Thus , RA is a potent but transient inhibitor of bovine mammary epithelial cell proliferation , and this growth inhibition is correlated with increased IGFBP 2 accumulation and inhibition of IGF 1 stimulated IGFBP 3 protein secretion . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
KCs synthesized IGFBP 2 , and 3 , insulin and IGF 1 showing no effect . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the control group , puberty occurred at about 120 days of age and was associated with an increase in concentrations of testosterone , IGF 1 and IGFBP 3 and a decrease in concentration of IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The cellular and sex steroid regulated pattern of IGFBP 2 gene expression is very similar to that of IGF 1 , as previously determined in these same myometrial samples . ^^^ Both IGF 1 and IGFBP 2 are dependent on E 2 for significant myometrial expression , and both are further augmented by the addition of P 4 to E 2 treatment . ^^^ The present observations , together with our previous data from the same animals , demonstrate that the primate myometrial smooth muscle cell expresses mRNAs for IGF 1 and 2 , IGF 1 and 2 receptors , as well as expresses mRNAs for IGFBP 2 , 3 , 4 , and 5 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 was detected in CM by probing Western blots with 125I IGF 1 ( ligand blot analysis ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , insulin and C peptide were measured by radioimmunoassay . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have studied steady state levels of mRNA for the genes in the IGF 1 axis in normal and cirrhotic human liver , localised the most highly expressed gene , IGFBP 1 , and measured circulating IGFBP 3 by radioimmunoassay ( RIA ) , IGFBP 2 and IGFBP 3 by Western ligand blot ( WLB ) , and protease activity for IGFBP 3 in cirrhotic patients . ^^^ Messenger RNA for IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 was detectable by Northern blotting in normal and cirrhotic liver although there was considerable variation in expression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In order to investigate the IGF regulatory pathway in leukaemia serum levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in 28 leukaemic children . ^^^ Whereas serum levels of IGF 1 ( mean / range : 2 . 7 / 0 . 1 to 6 . 7 SDS ) , IGF 2 ( 3 . 6 SDS / 1 . 3 to 8 . 7 ) and IGFBP 3 ( 2 . 0 / +2 . 2 to 7 . 1 SDS ) were significantly decreased comparable to levels in growth hormone deficiency , IGFBP 2 levels ( +4 . 0 / 0 . 45 to +7 . 4 SDS ) were found to be markedly elevated and inversely correlated to IGF 1 ( r = 0 . 51 , P = 0 . 013 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Whereas the enhanced endogenous production of glucocorticoids appears to mediate the IL 1 beta induced decrease in IGF 1 synthesis in liver , the changes in IGF 1 content observed in other tissues and the increase in IGFBP 1 and IGFBP 2 appear to be largely glucocorticoid independent . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Whole CL and luteal cells were extracted to isolate total or poly ( A ) + RNA , which was subjected to Northern and / or dot blot analyses using [ 32P ] labeled cDNA probes for IGF 1 and IGFBP 2 , 3 , 4 , 5 , and 6 . ^^^ Northern blots showed readily detectable transcripts for IGF 1 ( 6 . 7 and 0 . 9 kb ) , IGFBP 2 ( 1 . 8 kb ) , IGFBP 3 ( 2 . 8 kb ) , IGFBP 4 ( 2 . 6 kb ) , and IGFBP 5 ( 6 . 0 kb ) , but not for IGFBP 6 . ^^^ We conclude that porcine CL express mRNAs for IGF 1 and IGFBP 2 , 3 , 4 , and 5 , and that while small luteal cells are the major sources of IGF 1 and IGFBP 3 and 5 , IGFBP 2 and 4 appear to be expressed to approximately the same extent in small and large luteal cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma clearance of insulin like growth factors 1 and 2 ( IGF 1 and 2 ) and insulin like growth factor binding protein 2 ( IGFBP 2 ) from lactating goats ( n = 4 ) was determined following a single intravenous injection of the corresponding 125I labelled human protein . ^^^ Clearance of 125I IGFBP 2 from circulation was 0 . 37 + / 0 . 06 ml / min / kg , which is markedly greater than that of 125I IGF 1 or 2 ( 0 . 11 + / 0 . 01 and 0 . 12 + / 0 . 01 ml / min / kg respectively ) . ^^^ This was also reflected in longer elimination half lives for IGF 1 ( 353 + / 6 min ) and 2 ( 254 + / 8 min ) compared with IGFBP 2 ( 110 + / 9 min ) . ^^^ Three hours after injection of the 125I labelled protein , the plasma : lymph ratio of trichloroacetic acid precipitable radioactivity was 1 . 54 + / 0 . 04 , 3 . 3 + / 0 . 6 and 4 . 1 + / 0 . 4 for IGFBP 2 , IGF 1 and 2 respectively . ^^^ Elevation of plasma concentrations of IGFBP 2 by its intravenous infusion significantly decreased plasma half life of both IGF 1 and 2 ( 251 + / 8 and 198 + / 7 min respectively ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Free IGF 1 was inversely correlated ( r = 0 . 49 , P < 0 . 05 ) with IGFBP 1 and / or IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
On the initial evaluation , GH levels failed to rise in response to hypoglycemia , IGF 1 levels were low , IGF binding protein 3 ( IGFBP 3 ) levels were suppressed , and IGFBP 2 levels were elevated . ^^^ GH therapy resulted in a normalization of the levels of blood sugars , IGFBP 3 , ALS , IGFBP 2 , and IGF 1 , as well as the IGF / IGFBP 3 ratio . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 54 children with end stage renal disease ( ESRD ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We investigated the expression of the mRNAs encoding IGF 1 , IGF 2 , the type 1 IGF receptor ( IGF T1R ) and two IGF binding proteins ( IGFBP 2 and IGFBP 4 ) in rat lung during the perinatum . ^^^ At day 20 of gestation IGF 1 , IGF T1R and IGFBP 4 mRNA levels were higher in lung than liver ( all P < 0 . 01 ) , whereas IGF 2 and IGFBP 2 mRNA levels were higher in liver than lung ( each P < 0 . 02 ) . ^^^ The expression of IGF 1 , IGFBP 2 and IGFBP 4 in lung was high before birth ( days 17 20f ) but decreased to low levels at days 21f , 22f or at birth ( 1d ) but increased in the neonatal lung . ^^^ The patterns of expression of IGF 1 , IGF T1R , IGFBP 2 and IGFBP 4 , but not IGF 2 , in developing lung correspond to previously described phasic changes in lung cell proliferation rates . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 and IGFBP 1 both inhibit this IGF 1 receptor mediated stimulation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Recent studies demonstrate that IGF 2 in FFe is higher than in FFa ' whereas IGF 1 , IGFBP 3 and IGFBP 1 levels do not differ , underscoring the importance of local IGF 2 production by the granulosa and the importance of IGFBP 4 and IGFBP 2 in regulation of IGF 2 action within the follicle during its developmental pathway as an E 2 or A dominant follicle . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Protein restriction decreases plasma concentrations of albumin and insulin like growth factor 1 ( IGF 1 ) by reducing their hepatic mRNA levels , whereas protein restriction increases IGF 1 binding protein 2 ( IGFBP 2 ) gene expression in the liver . ^^^ The present study was designed to evaluate the effects of protein repletion and TNF on plasma albumin and IGF 1 and their mRNAs and IGFBP 2 mRNA in the liver of protein restricted rats . ^^^ Hepatic mRNAs of IGF 1 , albumin , and IGFBP 2 were determined . ^^^ Protein repletion increased plasma concentrations of IGF 1 and albumin and their mRNA content in the liver , but decreased IGFBP 2 mRNA . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured levels of maternal and cord serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 in 8 White Class B insulin dependent DP and 8 non diabetic pregnancies ( NP ) . ^^^ No significant differences were found between DP and NP with respect to cord serum IGF 2 , maternal serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 , and the concentration and affinity of TR for IN , IGF 1 and IGF 2 . ^^^ Regression analysis revealed significant correlations of cord IGF 1 with cord values of IGFBP 2 ( r = 0 . 52 , p = 0 . 04 ) and IGFBP 3 ( r = 0 . 66 , p < 0 . 005 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , insulin and IGFBP 1 were measured by specific RIAs , and IGFBP 2 and IGFBP 3 were quantified by densitometry after Western ligand blotting . ^^^ The ratio between IGF 1 and IGFBP 2 or IGFBP 3 did not change after dialysis . ^^^ In ESRD patients predialysis mean values of insulin , IGF 1 SD score , IGFBP 1 and IGFBP 2 were increased , while the mean densitrometric values of the IGFBP 3 bands on Western ligand blot were either normal or reduced . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor ( IGF ) binding protein 2 ( IGFBP 2 ) is secreted by several cell types that also secrete IGF 1 or IGF 2 . ^^^ The binding of IGF 1 or IGF 2 to IGFBP 2 has been shown to alter their actions . ^^^ In this study we report that IGFBP 2 binds to heparin if IGF 1 or IGF 2 is also included in the incubation buffer . ^^^ The binding of IGFBP 2 to heparin was detectable using an IGF 1 to IGFBP 2 molar ratio of 2 : 1 . ^^^ Binding appeared to be physiologically significant , as IGFBP 2 bound to human fibroblast ECM if IGF 1 or IGF 2 was added . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In summary , there is selective expression of IGF 1 , IGF 1 receptor , and IGFBP 2 and 4 mRNAs during the process of follicular selection in the gilt ovary , with IGFBP 4 expression being closely associated with follicular selection and luteinization . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Well controlled rats had normal cerebellar IGF 1 levels , whereas levels of IGFBP 2 protein and mRNA were still significantly low . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Cord sera were obtained from term , Chilean newborns exhibiting various patterns of intrauterine growth and assayed for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 by specific radioimmunoassays ( RIA ) . ^^^ By contrast , circulating IGFBP 1 and IGFBP 2 were inversely related to IGF 1 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Messenger RNA for GH receptor , IGF 1 , IGFBP 2 , IGFBP 3 , and actin were measured by nuclease protection assays . ^^^ The GH receptor mRNA was decreased in cows treated with GH whereas the mRNA for IGF 1 , IGFBP 2 , or IGFBP 3 was not changed . ^^^ In summary , the level of mRNA encoding GH receptor , IGF 1 , IGFBP 2 , and IGFBP 3 varied within the tissues examined , suggesting that these genes may play a variety of roles in the bovine female reproductive tract . ^^^ Supplemental GH failed to change the expression of IGF 1 , IGFBP 2 , and IGFBP 3 mRNA , possibly because of low GH receptor mRNA levels in tissues other than CL . ^^^ A direct action of GH on IGF 1 , IGFBP 2 , or IGFBP 3 gene expression within cow reproductive tissues was not supported because the amount of IGF 1 , IGFBP 2 , or IGFBP 3 mRNA was not altered by GH . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The mRNA for IGF 1 , IGF 2 , IGFBP 2 , LH receptor , FSH receptor , cytochrome P 450 side chain cleavage ( P450scc ) , 3 beta hydroxysteroid dehydrogenase ( 3 beta HSD ) , 17 alpha hydroxylase ( P 45017 alpha ) , and aromatase ( P450arom ) were detected by in situ hybridization , and relative amounts were determined by image analysis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both FSH and insulin like growth factor ( IGF 1 ) were necessary for maximum production of IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma prorenin and renin , serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein ( IGFBP 2 and IGFBP 3 ) concentrations were measured in 22 randomly selected male and female patients with insulin dependent diabetes mellitus ( IDDM ) or non IDDM ( NIDDM ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both treatments increase serum ALS , IGFBP 3 , and IGF 1 levels , and decrease IGFBP 2 , whereas IGFBP 6 is unaffected . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We also tested the ability of IGFBP 2 , a related binding protein which has an arginine glycine aspartate sequence but does not associate with integrin family members , to enhance IGF 1 bioactivity . ^^^ Full thickness dermal wounds were created on rabbit ears ; various combinations of native IGF 1 , native IGFBP 1 , native IGFBP 2 , and their respective analogues / mutants were applied to each wound . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Steady state hepatic IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 mRNA levels were measured by Northern blot analysis using specific rat cDNA probes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) Compared to 100 normal weight girls of same age the obese subjects had increased levels of IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 and normal levels of IGF 2 . ( 2 ) After weight loss ( 8 . 1 + / 2 . 0 kg ) IGF 2 and IGFBP 2 increased and IGF 1 , IGFBP 1 , IGFBP 3 and the ratio IGF I / IGFBP 3 decreased significantly . ( 3 ) Correlation analysis revealed that fasting insulin levels of the patients were positively correlated with IGF 1 but inversely with IGFBP 1 and IGFBP 2 . ^^^ The main conclusion is that obesity has to be considered for interpretation of serum concentrations of IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 receptor , and five IGF binding proteins ( IGFBPs ) , IGFBP 1 , IGFBP 2 , IGFBP 3 , IGFBP 4 , and IGFBP 5 in smooth muscle cells ( SMCs ) using colloidal gold immunocytochemistry . ^^^ Quantitative reverse transcription polymerase chain reaction ( QRT PCR ) performed on de novo atherectomy specimens identified mRNA for IGF 1 , IGF 1 receptor , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 levels and detected mRNA for IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , we report that EGF treatment for 4 weeks in Goettingen minipigs reduces circulating IGF 1 and IGFBP 3 , increases circulating IGFBP 1 , IGFBP 2 and IGFBP 4 , and induces a slight hyperthyroidism as judged from increased circulating levels of T3 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The rat uterus has been shown to be a site of production of insulin like growth factor 1 ( IGF 1 ) and multiple IGF binding proteins ( IGFBP 2 , 3 , 4 , 5 , 6 ) which are involved in estrogen induced uterine proliferation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
DNA synthesis appears to be in reciprocal relationship with hepatic expression of IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 4 , suggesting that IGF I / IGFBPs system is not involved in liver growth . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To understand the involvement of IGF 1 in Purkinje cell survival , we examined mRNA expression of IGF 1 , IGFR 1 , IGFBP 2 and 5 in the Purkinje cell degeneration ( pcd ) mice . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Fetal hepatic IGFBP 1 mRNA levels increased in 4 of 6 nephrectomized fetuses , while those of IGF 1 , IGF 2 , IGFBP 2 were unchanged . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Under basal conditions , SH SY5Y cells in serum free medium secreted IGF 2 , and traces of IGF 1 , IGFBP 2 and IGFBP 4 . ^^^ After 48 h , when IGFBP 2 and IGFBP 4 concentrations in the culture media had increased , des ( 1 3 ) IGF 1 remained the most active , but the activity of insulin now equalled or exceeded that of IGF 1 and IGF 2 . ^^^ This suggests a negative feedback mechanism involving partial sequestration of IGF 1 and IGF 2 by IGFBP 2 and IGFBP 4 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The presence of IGFBP 2 and 5 in the outer ciliary epithelium suggests secretion into the aqueous humour , where they may enhance trapping of IGF 1 which may be important for lens and corneal cell survival . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Forty three pediatric IDDM patients were randomly assigned to groups receiving daily , fasting subcutaneous injections of placebo or rhIGF 1 ( 80 micrograms . kg . day ) for 28 days , while continuing to receive splitmix insulin therapy and intensive outpatient management . rhIGF 1 therapy corrected IGF 1 deficiency , suppressed IGFBP 1 levels ( P < 0 . 01 ) , and induced a trend toward lower circulating GH levels throughout the study . rhIGF 1 therapy also induced an approximate 50 % decrease in IGF 2 levels ( P < 0 . 001 ) and an approximate 70 % increase in IGFBP 2 levels ( P < 0 . 05 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Messenger RNA for IGF 1 , IGFBP 2 , and IGFBP 4 was determined by solution hybridization . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Following a five day maternal fast , both IGF 1 and IGF 2 levels were decreased in the maternal and fetal circulation ( P < 0 . 05 ) , accompanied by a decrease in fetal hepatic IGF 1 mRNA levels , but the IGFBP 2 level was increased and the IGFBP 3 level was decreased in maternal circulation , whereas the IGFBP 1 level was increased in fetal circulation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
MEASUREMENT : Total IGF 1 , free IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and insulin were measured in obese subjects and normal control subjects . ^^^ The IGFBP 2 concentrations were also suppressed in obese subjects and inversely related to free IGF 1 ( r = 0 . 48 , P = 0 . 001 ) . ^^^ It seems likely that overnutrition and chronic hyperinsulinaemia in obesity may alter this regulated growth response by insulin stimulation of IGF 1 production and suppression of hepatic IGFBP 1 and IGFBP 2 production , which may inhibit IGF 1 bioactivity . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 ( 200 and 400 ng / ml ) inhibited ( p < 0 . 05 ) IGF 1 induced androstenedione production by 18 30 % but did not influence ( p > 0 . 10 ) progesterone production or thecal cell proliferation in the presence of LH and / or IGF 1 . ^^^ As with IGFBP 2 , IGFBP 3 had no effect ( p > 0 . 10 ) on LH induced progesterone or androstenedione production by thecal cells in the absence of IGF 1 . ^^^ Both IGFBP 2 and IGFBP 3 directly inhibited [ 125I ] IGF 1 and 2 binding to thecal cells ; IGFBP 2 was a weaker inhibitor of thecal [ 125I ] IGF 1 and 2 binding than IGFBP 3 . ^^^ These results indicate that IGFBP 3 has a more pronounced inhibitory effect than IGFBP 2 on IGF 1 action in cultured bovine thecal cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
After 12 months of study , the 30 children receiving rhGH showed : ( 1 ) greater increase in height ( 9 . 1 + / 2 . 8 vs . 5 . 5 + / 1 . 9 cm , P < 0 . 0001 ) ; ( 2 ) increases in serum levels of IGF 1 , IGF 2 , free IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) ; ( 3 ) a greater decrease in serum IGFBP 1 levels ; and ( 4 ) no significant difference in serum IGFBP 2 levels , when compared to the 14 control patients . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Immunological determination of IGFBP 2 , IGFBP 3 , IGF 1 , IGF 2 , interleukin 1 beta ( IL 1 beta ) and tumour necrosis factor alpha ( TNF alpha ) was undertaken in the SF and plasma of 115 patients with rheumatoid arthritis ( RA ; n = 53 ) , osteoarthritis ( OA ; n = 44 ) and other rheumatic disorders . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 2 , and 3 showed a congruency in sites of messenger RNA ( mRNA ) and peptide expression , with IGF 1 and IGFBP 2 mRNA expression predominating . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGF / IGFBP3 molar ratio , and IGF 1 and 2 binding capacities showed no significant change ; however in 5 of 6 patients IGFBP 2 decreased during deflazacort therapy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Spontaneous GH secretion , IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , their binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) values at the time of clinical diagnosis ( n = 65 ) , after a 25 % decrease in the body mass index ( BMI ) expressed as the SD score ( BMI SD score ; n = 29 ) , and after a diminution of at least 50 % of the initial BMI SD score ( n = 9 ) are reported . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We report their spontaneous GH secretion and IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , the IGF binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) levels at the time of the clinical diagnosis ( n = 50 ) and after recuperation of between 6 8 % ( n = 42 ) and 10 % or less of the initial weight ( n = 20 ) . ^^^ Serum IGFBP 2 levels had a strong negative correlation with fIGF 1 , IGF 2 , and the sum of IGF 1 and IGF 2 , but only at the time of diagnosis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Fasting levels of IGF 1 , free IGF 1 , IGF 2 , IGFBP 3 , and insulin increased significantly within 3 months of GH treatment , whereas IGFBP 1 , IGFBP 2 , and IGFBP 6 showed no change . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , the data point to IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 as putative mediators of relaxin induced uterine growth in the pig . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
By affinity chromatography on agarose IGF 1 and HPLC , truncated IGFBP 2 of apparent Mr 14 , 000 16 , 000 resolved into two peaks . ^^^ By solution binding assay , truncated IGFBP 2 showed less than 10 % binding of [ 125I ] IGF 1 and 25 % binding of [ 125I ] IGF 2 at pH 7 . 0 compared with rhIGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the patients , serum concentrations of IGFBP 3 were reduced , compared with controls , with a 46 % lower mean value ; whereas levels of IGF 1 , IGF 2 , IGFBP 2 , and GH were similar in the two groups . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
OBJECTIVE : IGF 1 and IGF binding protein ( IGFBP ) 3 levels in man are positively regulated by GH status ; in contrast , evidence suggests an inverse relationship between GH status and IGFBP 2 . ^^^ MEASUREMENTS : Serum concentrations of GH , IGF 1 , IGFBP 2 and 3 were determined in blood samples collected at 1600 h on the days prior to ( day 1 ) , during ( days 0 , 1 and 2 ) , and following administration ( days 3 and 7 ) . ^^^ The concentration ratios of IGFBP 3 to IGFBP 2 and IGF 1 to IGFBP 2 increased markedly with administration and both ratios were still significantly augmented compared with basal values 30 h after the last administration . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the tissues , IGFBP 2 mRNA was expressed in all normal and cancer samples but other IGFBPs showed variable expression . mRNAs for IGF 1 were expressed in all normal and cancer tissues but IGF 2 mRNA was only detected in cancer tissue ( 3 out of 10 ) . ^^^ Immunostaining of sections of normal and cancer tissue was negative for IGF 1 and IGF 2 ; IGFBP 2 was positive in 2 out of 10 cancer tissues and 7 out of 10 normal tissues ; IGFBP 3 was positive in 7 out of 10 cancer tissues and 7 out of 10 normal tissues ; and IGFBP 4 was positive in 5 out of 10 cancer tissues and 6 out of 10 normal tissues . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Circulating concentrations of IGF 1 and IGFBP 2 and 3 at wk 2 postpartum were indicators of the capacity of energy restricted cattle to resume cycling after parturition . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum levels of IGF 1 , but not those of IGFBP 2 or 3 , were significantly reduced in the postmenopausal group compared with the premenopausal group . ^^^ Serum levels of IGF 1 , but not those of IGFBP 2 or 3 , were positively correlated with BMD . ^^^ Serum levels of IGFBP 2 , but not those of IGF 1 or IGFBP 3 , were negatively correlated with body mass index and body weight . ^^^ The present findings suggest that a rapid decrease in serum IGF 1 levels after menopause might be partly involved in bone loss following gonadal failure and that IGFBP 2 and 3 might be related to the regulation of body mass and lipid metabolism during perimenopause respectively , although the mechanisms remain unknown . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 increased in calves fed only milk replacer and those receiving subcutaneous Long R 3 IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 antisense RNA also inhibits expression of IGFBP 2 in glioma cells , but does not influence expression of the other IGFBPs . ^^^ Although IGFBP 2 in conditioned medium from wild type C 6 cell cultures itself does not directly influence glioma cell growth , it synergistically enhances exogenous IGF 1 mediated DNA synthesis in IGF 1 negative C 6 cells . ^^^ IGF 1 dependent glioma cell growth may , in some circumstances , require IGFBP 2 as a cofactor . ^^^ The antitumor effect of IGF 1 antisense RNA is also associated with inhibition of IGFBP 2 expression . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IGF 1 , IGFBP 1 , and IGFBP 2 levels were measured by radioimmunoassay , and IGFBP 3 by immunoradiometric assay . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Furthermore , the expression of mRNAs coding for IGF 1 and the binding proteins IGFBP 2 and IGFBP 3 is augmented in response to unilateral ischemia in animal models . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There were significant inverse correlations between IGF 1 and both IGFBP 1 ( r = 0 . 45 ) and IGFBP 2 ( r = 0 . 62 ) . ^^^ According to multiple regression analysis free IGF 1 levels were only dependent upon total IGF 1 , IGFBP 2 and IGFBP 1 , whereas IGFBP 3 levels did not contribute to the variance of free IGF 1 concentrations in venous cord blood . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Binding of IGFBP 2 to chondroitin 6 sulfate decreased the binding affinity of IGFBP 2 for IGF 1 approximately 3 fold . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Furthermore , little or no data in these pathologies are available concerning IGFBP 2 or free IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The temporal variations in endometrial expression of mRNA encoding insulin like growth factor 1 ( IGF 1 ) and IGF 2 , and insulin like growth factor binding protein 1 ( IGFBP 1 ) and IGFBP 2 were investigated between oestrus and day 20 of pregnancy in ewes . ^^^ Northern blot analysis of endometrial total RNA revealed major transcripts for IGF 1 ( 7 . 1 kb ) , IGF 2 ( 5 . 8 kb ) , IGFBP 1 ( 1 . 3 kb ) and IGFBP 2 ( 1 . 7 kb ) . ^^^ The low endometrial expression of mRNA encoding IGF 1 at day 15 of pregnancy was used as the reference point for time comparison for expression of mRNA encoding IGF 1 , IGF 2 and IGFBP 2 . ^^^ Endometrial expression of mRNA encoding IGF 1 was increased ( P < 0 . 05 ) at oestrus and on day 8 of pregnancy relative to expression on day 15 , whereas expression of mRNA encoding IGFBP 2 was decreased ( P < 0 . 05 ) . ^^^ The temporal variation of the expression of mRNAs encoding IGF 1 and IGFBP 2 suggests a role for these factors in the uterine environment during early pregnancy in ewes coinciding with rapid development of the embryo and growth of the uterus in preparation for implantation . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We studied 24 severely depressed patients and 33 healthy controls and estimated 24 hour mean plasma cortisol , six hour evening mean plasma growth hormone ( GH ) , morning plasma IGF 1 , IGFBP 2 and 3 and GH binding protein ( GH BP ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study , testosterone , dihydrotestosterone ( DHT ) , 3 alpha androstanediol ( 3 alpha diol ) , IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 concentrations were evaluated in human benign prostatic hyperplasia ( BPH ) tissue . ^^^ IGF 1 , IGFBP 2 , and IGFBP 3 were measured by radioimmunoassay , and IGF 2 by immunoradio metric assay , after acidification and chromatography on Sep pak C 18 Cartridges for IGF 1 and IGF 2 . ^^^ IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels were 24 + / 3 . 7 , 121 + / 14 ng / g tissue and 0 . 44 + / 0 . 05 and 1 . 2 + / 0 . 17 micrograms / g tissue , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
As determined by specific radioimmunoassays SK N MC cells secreted 0 . 75+ / 0 . 02 ng / ml IGF 1 , 1 . 2+ / 0 . 04 ng / ml IGF 2 and 149+ / 2 . 1 ng / ml IGFBP 2 within 24 h , whereas CHP cells secreted 0 . 1+ / 0 . 01 ng / ml IGF 1 , but 6 . 2+ / 0 . 1ng / ml IGF 2 and 254 . 8+ / 5 . 5 ng / ml IGFBP 2 ( N=5 ) . ^^^ IGFBP 2 secretion correlated positively with IGF 2 secretion in CHP cells ( r=0 . 85 , P=0 . 05 ) and negatively with IGF 1 ( r= 0 . 9 , P < 0 . 01 ) in SK N MC cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The influence of IGF 1 on IGFBP 5 expression was specific because we found no evidence of changes in IGFBP 2 , IGFBP 4 , or cyclophilin expression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , in dominant and subordinate follicles were measured to determine if changes in IGF or IGFBP gene expression are associated with follicular dominance . ^^^ These data show that changes in gene expression for IGFBP 2 are opposite to those for IGF 1 or IGF 2 . ^^^ Increased IGF 1 and IGF 2 mRNA and decreased IGFBP 2 mRNA within the DF may be one mechanism leading to follicular dominance . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured IGFBP 2 , 4 and IGF type 1 receptor ( IGF 1 R ) mRNA by RT PCR , cell growth and IGFBP 2 secretion ( per 10 ( 6 ) cells ) . ^^^ The addition of 100 ng / ml IGF 2 enhanced the IGFBP 2 secretion 2 . 8 fold , while the use of IGF 1 only enhanced IGFBP 2 secretion 1 . 7 fold , although IGF 1 enhanced IGF 2 action . ^^^ Through inhibition using JB 1 , a peptide inhibiting the IGF signal transduction by blocking the IGF 1 R , we demonstrated the involvement of the IGF 1 R in IGFBP 2 and 4 expression and leukemic cell growth . ^^^ However , only slight differences in the IGF 1 R mRNA expression were seen for T and B cells compared with the differences found for the IGFBP 2 and 4 mRNA or IGFBP 2 secretion . ^^^ Thus , although IGF 1 R mediates the autocrine / paracrine effects of the IGFs , IGF 1 R mRNA expression is most probably not involved in the differential IGFBP 2 / IGFBP 4 expression in leukemic cells . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Analysis of the conditioned medium revealed that intestinal stromal cells secreted IGF 1 , but little IGF 2 , in addition to an M ( r ) 32 , 000 IGF binding protein ( IGFBP 2 ) and an IGFBP having M ( r ) approximately 24 , 000 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum immunoreactive IGFBP 3 levels fell and IGFBP 2 and 3 levels rose during IGF 1 therapy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured serum levels of free IGF 1 and free IGF 2 , total IGF 1 , total IGF 2 , big IGF 2 and IGFBP 1 , IGFBP 2 and IGFBP 3 in patients with NICTH before ( n=14 ) and after surgical removal of the tumour ( n=3 ) . ^^^ In accordance with earlier observations levels of total IGF 1 , total IGF 2 , and IGFBP 3 were decreased , whereas IGFBP 1 and IGFBP 2 were increased in NICTH ( all p values < 0 . 05 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Previously , we have reported that porcine VSMCs synthesize and secrete IGF 1 and several forms of IGFBPs , including IGFBP 2 , IGFBP 4 , and IGFBP 5 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The bacterially derived IGFBP 2 had a correct N terminal sequence , molecular mass on SDS PAGE and the same affinity for IGF 1 and IGF 2 as IGFBP 2 from mammalian cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We studied 11 elderly male ( 50 78 years ) marathon runners and 10 age matched male ( 52 73 years ) sedentary controls to evaluate plasma concentrations of GH , total and free IGF 1 and IGF 2 and of IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 and insulin . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In children without parenchymal disease , the growth factor expressed was IGF 1 , in association with the presence of mRNA for IGFBP 2 in all cases . ^^^ In children with ILD , expression of IGF 1 was observed in nine patients and of IGF 2 in three patients , and the presence of IGFBP 2 was found in all extracts analysed ( mRNA and proteins ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , IGFBP 2 had a similar pattern to PEPCK , and a periportal gradient of IGF 1 mRNA was also seen in the low protein group . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In addition , the differential regulation of IGFBP 2 , 3 and 6 by retinoic acid ( which inhibits proliferation ) and IGF 1 ( which stimulates proliferation ) suggests that these forms of IGFBP have different roles in regulating mammary epithelial cell physiology . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , no changes in the plasma concentrations of IGFBP 2 , 3 , or 4 were detected in alcohol fed rats at this time point Previous studies have indicated that elevations in glucocorticoids or decreases in insulin or growth hormone might be responsible for the decrease in IGF 1 and / or the increase in IGFBP 1 in other catabolic conditions . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Anti TGF beta increased concentrations of IGF 1 5 . 8 fold and IGFBP 2 and IGFBP 3 by 8 and 7 fold in conditioned media whereas IGFBP 4 decreased 5 fold . ^^^ Anti IGF 1 increased concentrations of IGFBP 2 and 3 by 9 and 35 fold , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
All subjects underwent a 24 h GH profile ( 20 minute sampling ) , measurement of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and growth hormone binding protein ( GHBP ) and , after an overnight fast , an AST ( intravenous arginine 20 g / m2 over 30 minutes ) . ^^^ Normative data for serum IGF 1 , IGF 2 , IGFBP 3 and IGFBP 2 were obtained from 125 subjects aged 60 87 years . ^^^ Serum IGF 1 , IGFBP 2 , and IGFBP 3 levels were significantly lower in the patients compared with the normal range , 102 ( 14 162 ) vs . 142 ( 59 298 ) micrograms / l ( P < 0 . 0001 ) , 415 ( 122 1868 ) vs . 640 ( 140 2585 ) micrograms / l ( P = 0 . 0007 ) and 2 . 29 ( 0 . 81 3 . 75 ) vs . 2 . 59 ( 1 . 00 3 . 52 ) mg / l ( P = 0 . 009 ) , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The present results show that ovarian hormones and dietary protein content affect the plasma concentrations of IGF 1 , IGFBP 3 and IGFBP 4 and hepatic mRNA of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 4 in different manners . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 and 5 are found in the apical ectodermal ridge ( AER ) , and IGF 1 and IGFBP 4 in the region of the zone of polarizing activity ( ZPA ) . ^^^ At 13 . 5 days post coitus ( dpc ) the IGF system colocalizes with apoptosis areas ; IGFBP 2 , 4 and 5 are found in the interdigital zone , while IGFBP 3 and IGF 1 border this region . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Brief training increased muscle volume in weight stable adolescent males and , surprisingly , influenced not only IGF 1 but GHBP and IGFBP 2 as well in a manner typically found in energy deficient states . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The IGF 1 ( granulosal cells ) and IGF 2 ( thecal cells ) are increased , whereas IGFBP 2 ( granulosal cells ) is reduced , in dominant follicles . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
GH , IGF 1 , IGFBP 3 and IGFBP 2 in cerebrospinal fluid of infants , during puberty and in adults . ^^^ During diagnostic lumbar punctions cerbrospinal fluid ( CSF ) was collected for the determination of GH , IGF 1 , IGFBP 3 and IGFBP 2 . ^^^ GH was not correlated to IGF 1 , IGFBP 3 or IGFBP 2 . ^^^ However , IGF 1 was strongly related to IGFBP 3 ( r = 0 . 529 ; < 0 . 001 ) and IGFBP 2 ( r = 0 . 796 ; < 0 . 001 ) as was IGFBP 3 to IGFBP 2 ( r = 0 . 685 ; < 0 . 001 ) , suggesting dependence of the three variables . ^^^ With IGFBP 3 or IGFBP 2 as control variables ( partial correlation ) IGF 1 was no longer related to the binding proteins , while the relation of IGFBP 3 to IGFBP 2 remained unchanged with IGF 1 as the control variable ( r = 0 . 687 ; < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These results indicate cell type specific expression for IGF 1 , IGFBP 2 , 3 , 5 and 6 after injury . ^^^ Our findings suggest that the IGF 1 produced by microglia after injury is transferred to perineuronal reactive astrocytes expressing IGFBP 2 . ^^^ Thus , modulation of IGF 1 action by IGFBP 2 might represent a key mechanism that restricts neuronal cell loss following HI brain injury . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The injured sciatic nerve expressed IGF 1 and IGF 2 as well as IGFBP 4 and IGFBP 5 , whereas central nervous system ( CNS ) scar tissue expressed IGF 1 , IGFBP 2 , and IGFBP 5 . ^^^ In line with the in vivo findings , cultured Schwann cells expressed IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 5 mRNAs , whereas cultured astrocytes expressed IGF 1 , IGFBP 2 , and IGFBP 5 mRNAs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Chronic LPS administration ( 250 microg / kg daily for 8 days ) significantly decreased body weight , serum levels of IGF 1 and pituitary GH content , whereas it increased circulating IGFBP 3 ( 47 kDa band ) , IGFBP 1 and IGFBP 2 ( 33 kDa band ) and the 24 kDa band ( which possibly corresponds to IGFBP 4 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
After oestrus , the overall means of both groups of ewes showed lower ULF IGF 1 content ( Days 3 and 12 ) , lower plasma IGF 1 concentrations ( Days 3 16 ) , higher endometrial expression of mRNA encoding IGFBP 1 ( Days 12 16 ) and lower endometrial expression of mRNA encoding IGFBP 2 ( Day 8 ) . ^^^ The concomitant endometrial expression of mRNA encoding IGFBP 1 and IGFBP 2 suggests a role of these binding proteins in the regulation of IGF 1 bioavailability in the uterine environment of the ewe . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
It is hypothesized that the observed increase of IGF 1 in medium likely resulted from protective sequestration of IGF 1 by IGFBP 2 rather than from enhanced IGF 1 secretion . ^^^ In summary , these data suggest that multiple passaging of cloned porcine satellite cells results in increased secretion of IGFBP 2 , which is associated with depressed cell proliferation and myotube formation , perhaps because the increased IGFBP 2 sequestered IGF 1 and reduced its bioactivity . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 2 ( IGFBP 2 ) , the predominant IGFBP in the fetal circulation and an induced protein during several types of malignancies , belongs to a family of structurally related proteins that bind the mitogens , IGF 1 and IGF 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The lower growth activity measured in the IGFBP 2 secreting clones was compensated in great part by the administration of exogenous IGF 1 or 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured lower leg length and markers of bone formation [ bone alkaline phosphatase ( ALP ) and procollagen type 1 C terminal propeptide ( PICP ) ] , bone resorption [ pyridinoline , deoxypyridinoline , and carboxyl terminal telopeptide of type 1 collagen ( ICTP ) ] , soft tissue turnover [ procollagen type 3 N terminal propeptide ( P3NP ) ] , and the GH axis [ IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 2 , and urinary GH ] at 1 to 4 week intervals from diagnosis to week 27 of treatment . ^^^ At diagnosis , mean SD scores were : bone ALP , 1 . 84 ; PICP 1 . 77 ; pyridinoline , 1 . 42 ; deoxypyridinoline , 1 . 66 ; ICTP , 0 . 42 ; P3NP , +1 . 45 ; GH , +24 . 4 ; IGF 1 , 1 . 70 ; IGFBP 3 , 0 . 88 ; IGFBP 2 , +2 . 42 ; and GH binding protein , 0 . 69 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Other results were : IGF 1 , 3 11 ng / mL ( normal , 114 492 ) ; IGF 2 , 185 299 ng / mL ( normal , 358 854 ) ; IGF binding protein 1 ( IGFBP 1 ) , 12 200 ng / mL ( normal , 13 73 ) ; IGFBP 2 , 60 384 ng / mL ( normal , 55 480 ) ; and IGFBP 3 , 400 600 ng / mL ( normal , 2000 4000 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
C 6 cells produce IGFBP 3 , IGFBP 4 , a negligible amount of IGFBP 2 , and IGF 1 . ^^^ The clones , which overexpressed high levels of IGFBP 2 and IGF 1 , had growth rates similar to the wild type cells , whereas the three clones that overexpressed IGFBP 2 without a concomitant increase in IGF 1 had reduced growth rates . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSION : In conclusion , in contrast to previous reports , the present data do not confirm a correlation between serological or histological markers of renal osteodystrophy and circulating IGF 1 or 2 or IGFBP 2 and 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In addition to a direct stimulation of IGF 1 and ALS expression , GH inhibits IGFBP 1 serum levels and IGFBP 2 expression in an indirect manner , possibly facilitating enhanced IGF bioavailability to target tissues . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We studied the influence of 24 hour hypoxia ( 10 % oxygen ) , 48 hour hyperoxia ( 75 % oxygen ) , and relative hypoxia ( shifting from 48 hours in 75 % oxygen to 24 hours in room air ) on the gene expression of IGF 1 , IGF 1 receptor ( IGF IR ) , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 in retina using a solution hybridization RNase protection assay . ^^^ Hypoxia induced a significant increase in retinal IGF IR ( 178 % ) , IGFBP 2 ( 227 % ) , and IGFBP 3 ( 317 % ) mRNA ; however , retinal IGF 1 mRNA was reduced , as well as serum growth hormone ( GH ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
This study represents the most comprehensive and detailed analysis of the physiology and anatomy of the mouse ovarian IGF system , and shows that 1 ) IGFBP 5 is linked to the survival of the slow growing and immature preantral follicles ; 2 ) IGF 1 is associated with the growth and survival of the rapidly growing large preantral and antral follicles ; 3 ) IGFBP 4 is an atretogenic candidate for mouse ovarian follicles ; 4 ) ovulatory doses of PMSG / hCG up regulate IGFBP 2 mRNA expression in the ovarian interstitium ; and 5 ) transcripts of IGF type 1 receptor and IGFBP 2 through 5 , but not those of IGF 1 are highly expressed in the mouse corpus luteum . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , Nx did not alter the levels of IGF 1 , IGFBP 2 , and IGFBP 3 mRNA . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We evaluated the GH bioactivity ( GH BIO ) , GH serum level by immunofluorimetric assay ( GH IFMA ) , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 in 21 prepubertal obese children ( 13 boys and eight girls ) aged 5 . 7 to 9 . 4 years affected by simple obesity and in 32 ( 22 boys and 10 girls ) age and sex matched normal weight controls . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CyA did not affect CF secretion of transforming growth factor beta 1 , but markedly stimulated insulin like growth factor 1 ( IGF 1 ) secretion and inhibited secretion of both IGF 1 binding protein ( IGFBP ) 3 and IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In liver and gastrocnemius muscle , mRNAs for IGF 1 , IGFBP 2 , and IGFBP 3 were analyzed in young and aged males of two strains , BN / F344 and Sprague Dawley . ^^^ In aged Sprague Dawley males , liver mRNA for IGF 1 was increased 15 % and muscle mRNA for IGFBP 2 was increased 110 % . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
As for the peripheral limb of the GH insulin like growth factor 1 ( IGF 1 ) axis , high free IGF 1 , low IGF binding proteins 1 ( IGFBP 1 ) and 2 ( IGFBP 2 ) , normal or high IGFBP 3 and increased GH binding protein ( GHBP ) circulating levels have been described in obesity . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Leptin , insulin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured by specific immunoassays . ^^^ There were also significant increments in insulin , IGF 1 , and IGFBP 3 , whereas IGF 2 , IGFBP 1 , and IGFBP 2 did not change over 9 months of GH treatment . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In AN , the levels of GH binding protein , free and total IGF 1 , free IGF 2 , and IGFBP 3 were significantly reduced ; total IGF 2 , IGFBP 2 , and IGFBP 4 levels were unchanged ; and IGFBP 1 was increased . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSION : Twice daily rhIGF 1 therapy in adults with type 1 diabetes resulted in an increase in circulating IGF 1 with a reciprocal decrease in IGF 2 , and a marked elevation of IGFBP 2 concentration . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSION : GH significantly increased serum IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 4 and decreased serum IGFBP 1 and IGFBP 2 in post menopausal women . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
During IEC 6 growth , the autocrine release of IGF 1 and 2 increased , whereas IGFBP 2 levels decreased . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 ( 16 nM ) also inhibited the growth response to IGF 1 , but this effect could only be obtained if the two peptides were pre incubated together for 2 h prior to addition to the cells . ^^^ In conclusion , IGFBP 1 , 2 and 4 , of which IGFBP 2 and 4 may be locally derived , act as inhibitors with different potencies on IGF 1 effects in VSMCs . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Furthermore , we measured serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in the patients . ^^^ The serum IGFBP 2 , IGF 1 , and IGFBP 3 levels returned toward normal ranges as BMI in the patients improved during treatment . ^^^ The present study suggested that ALS was regulated by nutritional status , the same as IGF 1 , IGFBP 2 and IGFBP 3 ; but the serum IGFBP 2 was the best predictor of BMI as nutritional status marker among the parameters in patients with anorexia nervosa . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Competitive binding studies indicated that IGFBP 2 fragments had reduced binding affinity for IGF 1 . bFGF induced IGFBP 3 mRNA and protein . ^^^ Affinity cross linking of [ 125I ] IGF 1 to neuroblastoma cell membranes followed by immunoprecipitation revealed a approximately 38 kDa [ 125I ] IGF I / IGFBP 2 complex . ^^^ Cell surface associated IGFBP 2 was also susceptible to bFGF induced proteolysis , with the appearance of a single cross linked 21 kDa complex with low affinity for IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The concentrations measured by RIA on d 3 were 10 . 7+ / 0 . 4 ng / mL for IGF 2 , 1 . 9+ / 0 . 1 ng / mL for IGF 1 , 100+ / 5 ng / mL for IGF binding protein 3 ( IGFBP 3 ) , and 2163+ / 108 ng / mL for IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In active MS lesions , the expression of IGF 1 , insulin and IGFBP 1 was detected in hypertrophic astrocytes while that of IGF 2 and IGFBP 2 and 3 was confined to foamy macrophages within lesions and activated microglia in adjacent white matter . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Addition of a neutralizing antibody to insulin like growth factor 1 ( IGF 1 ) during PTH treatment resulted in the inhibition of PTH stimulated aggrecan synthesis , whereas the addition of a neutralizing antibody to insulin like growth factor binding protein 2 ( IGFBP 2 ) resulted in an increase in synthesis in both the control and PTH treated cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated gene expression of IGFBP 2 and IGFBP 4 . ^^^ ANG 2 decreased IGF 1 , IGFBP 2 , and IGFBP 4 transcripts but increased the IGF 1 receptor transcript . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The addition of an excess of IGF 1 enhanced IGFBP 4 proteolytic degradation , whereas addition of IGFBP 2 or 3 or monoclonal antibodies against IGF 1 and 2 dose dependently inhibited IGFBP 4 proteolytic degradation . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
MEASUREMENTS : IGFBP 1 and insulin were measured in samples drawn every 4 h over the 24 h period while IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in samples collected at the end of the study . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Patient serum contained low levels of IGF 1 , IGFBP 3 and ALS , while the concentrations of IGFBP 2 and IGFBP 6 were markedly elevated . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , our results suggest that IGF 1 , the type 1 IGF receptor and IGFBP 2 inhibit apoptosis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased IGFBP 3 , IGFBP 2 and decreased IGFBP 4 , while TGF beta 1 decreased IGFBP 3 and apparently increased IGFBP 4 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The genes for IGF 1 and 2 , type 1 IGF receptor , IGFBP 2 , and IGFBP 5 are expressed in both granulosa and theca cells of the chicken ovary . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We therefore measured IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 , and acid labile subunit ( ALS ) in 27 GHD subjects ( aged 5 82 yr ) from an extended kindred in Northeast Brazil with an identical GHRH receptor mutation and in 55 indigenous controls ( aged 5 80 yr ) . ^^^ The proportion of IGF 1 associated with IGFBP 3 remained constant throughout life , but was significantly lower in GHD due to an increase in IGF I / IGFBP 2 complexes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The high nutritional status of pigs in the early phases of growth diminished the postnatal changes in IGF 1 and IGFBP 2 , but not IGF 2 or IGFBP 3 . ^^^ Overall , data demonstrate a developmental regulation of the ST / IGF system , with pST challenges altering circulating concentrations of IGF 1 , IGFBP 3 , and IGFBP 2 coincident with changes in amino acid metabolism . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , since IGFBP 2 and 3 protein levels increased early in the treatment period ( Days 6 7 ) , it is possible that they may mediate the luteolytic actions of PGF ( 2alpha ) by sequestering IGF 1 and preventing its interaction with the IGF IR . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IGFBP 2 levels of CMV IGFBP 2 transgenic mice were about 3 fold ( P < 0 . 05 ) increased , compared with controls , whereas serum levels of IGF 1 and IGF 2 were unaffected by IGFBP 2 overexpression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In addition , 5alpha DHT decreased IGFBP 4 mRNA and protein levels by 2 and 4 fold , respectively , and increased IGFBP 2 and 3 mRNA and protein levels by 6 and 7 fold ( for mRNA ) and 3 and 5 fold ( for protein ) , respectively . hFOB / AR 6 cells expressed the type 1 IGF receptor , but this was not regulated by 5alphaDHT . 5alphaDHT and IGFBP 3 specifically increased hFOB / AR 6 cell proliferation , and a monoclonal antibody specific for IGF 1 blocked this effect . ^^^ Thus , androgens increase the expression of IGF 1 , IGFBP 2 , and IGFBP 3 , but decrease levels of the inhibitory IGFBP 4 in an androgen responsive human osteoblastic cell line . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
DESIGN , PATIENTS AND MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , osteocalcin ( OST ) , bone specific alkaline phosphatase ( BAP ) , urinary deoxypyridinoline ( DPYD ) and N telopeptide of type 1 collagen ( NTX ) were measured in 31 premenopausal women aged 31 43 and 65 postmenopausal women aged 47 60 : this latter group comprised 30 normal healthy women and 35 osteoporotic women . ^^^ RESULTS : Compared with premenopausal women or normal postmenopausal women , serum IGF 1 and IGFBP 3 levels were significantly lower in osteoporotic postmenopausal women while no significant differences in serum levels of IGF 2 , IGFBP 1 and IGFBP 2 were observed . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Chinese hamster ovary cells stably transfected with the zebrafish IGFBP 2 cDNA secreted a 31 kDa protein , which bound to IGF 1 and IGF 2 with high affinity , but did not bind to Des ( 1 3 ) IGF 1 or insulin . ^^^ When overexpressed or added to cultured zebrafish and mammalian cells , the zebrafish IGFBP 2 significantly inhibited IGF 1 stimulated cell proliferation and DNA synthesis . ^^^ These results indicate that zebrafish IGFBP 2 is a negative growth regulator acting downstream in the growth hormone IGF 1 axis . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Immunoreactivity for IGF 1 and IGFBP 2 was markedly increased in these Bcl 2 expressing mitral cells compared to wildtype mice . ^^^ In newborn IGF 1 transgenic mice , cerebellar Purkinje cells overexpressing IGF 1 showed markedly increased immunoreactivity for Bcl 2 and IGFBP 2 . ^^^ These studies indicate that in the developing brain IGF 1 modulates expression of its major binding protein IGFBP 2 , as well as the Bcl 2 protein . ^^^ In addition apoptosis caused by culturing OBs in SF medium , is inhibited by expression of Bcl 2 in the mitral neurons and is associated with enhanced expression of the IGF system , including IGF 1 and IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The Ati Negritos showed lower growth hormone binding protein ( GHBP ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) , zinc , albumin , ferritin , iron , iron saturation and much higher insulin like growth factor binding protein 2 ( IGFBP 2 ) and plasma transferrin concentrations . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated the cells to grow to a high cellular density and inhibited IGFBP 2 secretion in a concentration dependent fashion . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
As this IGF 1 analogue has lower affinity for IGFBPs , we believe that in this cell culture system , activity of IGF 1 may be attenuated in the long and short term by the accumulation of IGFBP 2 in conditioned medium and by the presence of IGFBP 2 associated with the cell membrane and / or ECM . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , some changes became accentuated ( GHBP and IGFBP 2 ) , while others ( free IGF 1 and IGFBP 1 ) were no longer present . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Fasting serum levels of IGFBP 1 , IGFBP 2 , IGFBP 3 , IGF 1 and free IGF 1 were determined using appropriate immunoenzymatic , radioimmuno or immunoradiometric assays . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : The patients had reduced serum free IGF 1 ( 53 % ) and increased levels of total IGF 2 ( 40 % ) , IGFBP 1 ( 546 % ) , and IGFBP 2 ( 270 % , P < 0 . 05 ) . ^^^ In patients , free IGF 1 levels correlated positively with CCr ( r 2 = 0 . 38 , P < 0 . 002 ) and inversely with IGFBP 1 ( r 2 = 0 . 69 , P < 0 . 0001 ) and IGFBP 2 ( r 2 = 0 . 41 , P < 0 . 0007 ) , whereas CCr was inversely correlated with levels of IGFBP 1 ( r 2 = 0 . 48 , P < 0 . 0001 ) and IGFBP 2 ( r 2 = 0 . 63 , P < 0 . 0001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have extended the analysis of specimens to include measurements of GHBP , PGH , IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 and have related these to maternal characteristics , fetal growth , and glycemia . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In all subjects , free and total IGF 1 levels showed significant negative correlations with IGFBP 1 , IGFBP 2 , and IGFBP 4 ( that is , inhibitory IGF system components ) and significant positive correlations with IGFBP 3 and IGFBP 5 ( that is , stimulatory IGF system components ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Circulating IGF 1 was measured by RIA whereas plasma IGFBPs was evaluated by WLB ; IGFBP 2 was additionally quantified by RIA procedure . ^^^ Surprisingly , although differences were detected for IGF 1 levels between the three groups , the IGFBP 2 and 3 data , evaluated by WLB could only discriminate between NG and SG group and not between NG and RG . ^^^ However , by using a RIA method , an IGFBP 2 decrease was observed in the NG group coincident with increasing IGF 1 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured basal levels of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 together with 24 h urinary GH excretion . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 ( an IGF 1 dependent responsive variable ) rose from 141 + / 56 to 251 + / 98 ng / mL ( P < 0 . 01 ) on IGF I / IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
CONCLUSION : Changes in circulating IGFBP 2 and IGFBP 3 paralleled the changes reported in non steroid treated infants ; however , it remains uncertain whether the natural rise in IGF 1 was suppressed by DEX treatment . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Tissues were immunostained for IGF 1 and IGFBP 2 , 3 , 4 , and 5 , and staining was quantitated by computerized image analysis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , the plasma IGF 1 and insulin status are markedly , albeit transiently , decreased in calves fed colostrum with a delay of 12 to 24 h , and the decreased concentrations of plasma IGF 1 were associated with decreased IGFBP 3 / IGFBP 2 ratios . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Baseline serum IGF 1 and IGFBP 3 were low , while IGFBP 1 and IGFBP 2 were raised . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Studying deficient growth in this hypoplastic infant ( birth weight 1405 g , birth length 36 cm ) with dysmorphic features , the following changes in IGFs and IGFBPs were detected ( microg / l ) : IGF 1 : 26 . 5 vs 48 . 1 in healthy infants ; IGF 2 : 420 vs 728 ; IGFBP 2 : 931 vs 524 ; IGFBP 3 : 800 vs 1070 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULT ( s ) : The IGF 1 levels in peritoneal fluid were significantly higher in patients with endometriosis than in control patients , while the IGFBP 3 levels and the relative proportion of IGFBP 2 in peritoneal fluid were significantly lower in patients with endometriosis than in control patients . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Using RNAase protection analysis ( RPA ) , we examined the gene expression for IGF 1 , IGF 2 , IGF1R , M6P / IGF2R , IGFBP 1 and IGFBP 2 in a series of hepatoblastomas with corresponding normal liver from the same individuals . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The changes in serum IGFBP concentrations ( eg , increases in IGFBP 1 and IGFBP 2 ) may lead to alterations in the availability of IGF 1 to peripheral tissues . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These ovarian differences may be due to changes in the pattern of secretion of FSH , insulin , IGF 1 , and GH but not LH , IGF 2 , or IGFBP 2 or 3 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , IGFBP 2 or 3 or monoclonal antibodies against IGF 1 or 2 dose dependently inhibited IGFBP 4 degradation , and IGF 1 or 2 reversed this inhibition in a dose dependent manner . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Since the growth hormone ( GH ) / IGF system is involved in a number of pathological alterations of the kidney and these changes may at least in part be due to increased IGF 1 , local overexpression of inhibitory IGFBP 2 in the kidney might prevent IGF 1 induced lesions . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have used the mouse mutant Hypodactyly ( Hd ) as a tool to determine the role of IGF 1 and two key IGF binding proteins ( IGFBP 2 and IGFBP 5 ) during embryonic development . ^^^ We show that all three genes , IGF 1 , IGFBP 2 and IGFBP 5 , display restricted expression patterns during limb development . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 1 and IGFBP 2 mRNAs were not detected in PT and PD . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We conclude that increased expression of IGF 1 in muscle results in increased IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The mitogenic effect of IGF 1 could be inhibited by both IGFBP 2 and IGFBP 3 showing that these binding proteins modulate the bioactivity of IGF 1 in the mammary gland at the cellular level . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These contrasting backgrounds led us to investigate the relationship between serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and the presence of colorectal adenomas , known precursors of colorectal carcinoma , in 345 volunteers attending a screening flexible sigmoidoscopy trial ( entry criteria : healthy , aged 55 64 yr ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Significant correlations ( p < 0 . 001 ) were seen between total and free ALS concentrations and IGF 1 ( r = 0 . 50 and 0 . 60 , respectively ) , free IGF 1 ( r = 0 . 37 and 0 . 36 ) , IGF 2 ( r = 0 . 37 and 0 . 27 ) , IGFBP 1 ( r = 0 . 48 and 0 . 49 ) , IGFBP 2 ( r = 0 . 44 and 0 . 51 ) and IGFBP 3 ( r = 0 . 67 and 0 . 59 ) at all Tanner stages . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of testosterone were negatively correlated ( P < . 01 ) with relative amounts of serum IGFBP 2 but positively correlated ( P < . 01 ) with serum concentrations of IGF 1 and estradiol 17beta . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The lower plane of nutrition decreased the expression of the ALS , IGF 1 , and GH receptor genes and increased the expression of the IGFBP 2 gene ; expression of the IGFBP 3 gene was not affected by nutrition at this stage of life . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin , IGF 1 and IGFBP 2 levels in follicular fluids from large follicles did not differ between females whereas IGFBP 3 levels were lower in L 1 than in H gilts ( P < 0 . 05 ) and intermediate in H 1 gilts . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Despite the hyperthyroid induced elevations in IGFBP 2 and 3 , no significant difference in the serum concentration of free IGF 1 before or after correction of the hyperthyroid condition was observed . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We conclude that 1 ) males are significantly more responsive than females to exogenous GH ; 2 ) the increase in IGF 1 is more robust compared with those in IGFBP 3 and ALS ; 3 ) IGFBP 2 changes very little during GH treatment ; and 4 ) among IGF related substances , IGF 1 is the most specific marker of supraphysiological GH exposure . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both types of patients have increased serum IGF 1 and IGFBP 2 levels , and decreased serum IGFBP 1 levels , while only AT patients have high serum insulin levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The aims of our study were to investigate : ( 1 ) the developmental pattern of the serum IGFBP 2 concentration at birth and during childhood and adolescence ; ( 2 ) whether the serum IGFBP 2 level could be a marker for the diagnosis and evolution of diseases where the growth hormone ( GH ) IGF axis is altered , and ( 3 ) whether this binding protein shows a relationship with IGF 1 , its free fraction , IGFBP 1 and 3 . ^^^ IGFBP 2 correlated positively with IGFBP 1 and negatively with IGF 1 and IGFBP 3 in normal subjects and at diagnosis of the pathologies studied . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Significance of basal IGF 1 , IGFBP 3 and IGFBP 2 measurements in the diagnostics of short stature in children . ^^^ In comparing IGF 1 , IGFBP 3 and IGFBP 2 serum levels of GHD and ISS children with reference data , we calculated the sensitivity , specificity , efficiency and positive predictive value for the diagnosis of GHD . ^^^ The overall sensitivity of the parameters was high , the rank order being as follows : IGF 1 > IGFBP 3 > IGFBP 2 ( 75 , 67 and 62 % , respectively ) . ^^^ In contrast , the specificity was relatively low : IGFBP 3 > IGFBP 2 > IGF 1 ( 50 , 50 and 32 % , respectively ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We also found coinduction of several groups of related genes that might represent functional modules within the ischemic neuronal transcriptome , including VEGF and its receptor , NRP 1 ; the IGF 1 receptor and the IGF 1 binding protein IGFBP 2 ; Rb , the Rb binding protein E2F1 , and the E2F related transcription factor , TFDP 1 ; the CACNB 3 and CACNB 4 beta subunits of the voltage gated calcium channel ; and caspase 3 and its substrates , ACINUS , FEM 1 , and GSN . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor ( IGF ) 1 , IGF 2 , free IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 and IGFBP 3 were measured by radioimmuno / immunoradiometric assays . ^^^ While this study confirms previous observations during treatment with oral oestrogens in substitution doses , the reduction in plasma IGF 2 , free IGF 1 , IGFBP 2 and 3 are all novel findings . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 by immunoassays , IGFBP 3 by Western ligand blotting ( WLB ) and finally in vitro IGFBP 3 proteolysis by a ( 125 ) 1 IGFBP 3 degradation assay . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
METHODS : To evaluate the role of the IGF system in lymphangioleiomyomatosis ( LAM ) , we used immunohistochemical and in situ hybridization techniques to characterize the expression of IGF 1 , IGF 2 , IGF 1R , and IGFBP 2 , 4 , 5 , and 6 in lung tissue from 18 LAM patients . ^^^ RESULTS : IGF 1 , ICGF 2 , IGF 1R , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 , and IGFBP 6 were expressed by LAM cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Blood levels of IGF 1 and IGFBP 3 were reduced before the diet in all patients while ligand blotting showed that IGFBP 2 and 1 were increased . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Relevance of IGF 1 , IGFBP 3 , and IGFBP 2 measurements during GH treatment of GH deficient and non GH deficient children and adolescents . ^^^ AIM / METHOD : Our aim was to monitor the serum levels of IGF 1 , IGF binding protein ( IGFBP ) 3 , and IGFBP 2 during long term GH treatment of 156 patients with GH deficiency ( GHD ) and of 153 non GHD patients . ^^^ Our analysis showed that , in both groups , in addition to maximum GH , all IGF parameters ( IGF 1 , IGFBP 3 , IGF I / IGFBP 3 ratio , IGFBP 2 or derivatives ) significantly extend the scope of a calculated model for predicting 1st year height velocity . ^^^ CONCLUSION : For reasons of safety and optimization of GH therapy , it is essential to follow up IGF 1 , IGFBP 3 , and IGFBP 2 levels regularly during childhood . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured levels of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and insulin in plasma samples from 149 men who had a diagnosis of prostate cancer between 1 month and 10 years after blood collection and among 298 control men . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Both halves of bIGFBP 2 bound IGF 1 and IGF 2 in BIAcore studies , albeit with different affinities ( ( 1 132 ) IGFBP 2 , K ( D ) = 36 . 3 and 51 . 8 nm ; ( 136 279 ) IGFBP 2HIS , K ( D ) = 23 . 8 and 16 . 3 nm , respectively ) . ^^^ Furthermore , des ( 1 3 ) IGF 1 and des ( 1 6 ) IGF 2 exhibited reduced binding affinity to ( 1 279 ) IGFBP 2HIS , ( 1 132 ) IGFBP 2 , and ( 136 279 ) IGFBP 2HIS biosensor surfaces compared with wild type IGF . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The assay was highly specific : no signal was obtained unless both IGF 1 and IGFBP 1 were present and neither IGFBP 2 , 3 , 4 , nor IGF 2 caused any cross reaction . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
If exogenous and secreted IGFBP 2 must bind to CCL 64 cells to inhibit DNA synthesis , Leu ( 60 ) IGF 1 might reduce the inhibition of DNA synthesis by bIGFBP 2 or TGF beta by inhibiting the association of IGFBP 2 in the media with CCL 64 cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma concentrations of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and C peptide were determined . ^^^ RESULTS : Mean concentrations of plasma IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and C peptide were 156 . 1 , 14 . 3 , 434 . 4 , 3062 , and 2 . 86 ng / ml , respectively . ^^^ Women in the highest tertile for physical activity had lower plasma concentrations of IGF 1 , IGFBP 3 , and C peptide , and higher concentrations of IGFBP 1 and IGFBP 2 , as compared to women in the lowest tertile . ^^^ We did confirm that a lean body shape is associated with higher concentrations of IGFBP 1 and IGFBP 2 , and possibly also with lower concentrations of IGF 1 and IGFBP 3 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : Plasma levels of IGF 1 , IGFBP 2 , and IGFBP 3 were measured preoperatively in 120 consecutive patients who underwent radical prostatectomy for clinically localized disease , postoperatively in 51 of these patients , in 44 healthy men , in 19 patients with metastases to regional lymph nodes , and in 10 patients with bone metastases . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , the response of IGFBP 2 gene expression to variations in nutritional status was rapid and different in several tissues of young chickens , which would help modulate the growth promoting effect of circulating IGF 1 by making the IGF IGFBP complex . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The results of GH provocative tests were compared to age and gender based standard deviation scores ( SDS ) of plasma IGFBP 2 , IGF 1 , IGFBP 3 and the molar ratios of the latter two to IGFBP 2 . ^^^ A weak negative relationship was found between IGFBP 2 and IGF 1 . ^^^ The use of various combinations of IGFBP 2 , IGF 1 , IGFBP 3 and the derived ratios only slightly improved the diagnostic efficiency as compared to the results of the individual tests . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We examined the changes in plasma IGF 1 concentration and tissue IGFBP 2 gene expression of young fasted chickens refed a commercial diet or administered bovine insulin intravenously . 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were measured in healthy adults and acromegalics . ^^^ RESULTS : For acromegalic patients , GH was significantly correlated with IGF 1 ( r = 0 . 50 ; P < 0 . 001 ) and IGFBP 3 ( r = 0 . 29 ; P = 0 . 03 ) but not IGF 2 or IGFBP 2 . ^^^ The ( IGF 1 + IGF 2 ) / IGFBP 3 molar ratio appears to be pivotal in determining IGF 2 values , which , in turn , expressed as a ratio of IGFBP 3 , is related to IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased the concentration of IGFBP 2 and 3 and decreased the concentration of IGFBP 4 in the medium of A 549 cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
BMD in total body , distal and UD radius , and femoral neck was positively correlated with serum IGF 1 ( r = 0 . 31 to 0 . 49 ) , IGF 2 ( r = 0 . 32 to 0 . 48 ) , IGFBP 3 ( r = 0 . 37 to 0 . 53 ) , and free androgen index ( FAI ) ( r = 0 . 32 to 0 . 40 ) , and negatively with IGFBP 1 ( r = 0 . 37 to 0 . 41 ) and IGFBP 2 ( r = 0 . 29 to 0 . 41 ) levels . ^^^ Age correlated negatively with serum IGF 1 , IGF 2 , IGFBP 3 , FAI , estradiol / SHBG ratio , and BMD in total body , distal and UD radius , and femoral neck , and positively with IGFBP 1 , IGFBP 2 , and SHBG levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Roles of insulin like growth factor 1 ( IGF 1 ) and IGF 1 binding protein 2 ( IGFBP 2 ) and 5 ( IGFBP 5 ) in developing chick limbs . ^^^ We have examined the distribution of IGF 1 , IGFBP 2 and IGFBP 5 in developing chick limb buds and have investigated their functional roles and relationships during chick limb development . ^^^ IGF 1 and IGFBP 2 are co expressed throughout the lateral plate from which limbs form , although IGFBP 2 , unlike IGF 1 , does not promote formation of rudimentary limb buds from non limb forming flank regions in vitro . ^^^ During skeletogenesis , IGF 1 and IGFBP 2 are co localized to the condensing central core of the limb , implicating these factors as potential regulators of the onset of chondrogenic differentiation . ^^^ Intriguingly , we found that IGF 1 and IGFBP 2 have opposing effects on chondrogenesis , as IGF 1 stimulates but IGFBP 2 inhibits accumulation of cartilage matrix by micromass cultures in vitro . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Relative to uninjected cattle , treatment with bST increased ( P < 0 . 05 ) serum concentrations of IGF 1 and IGFBP 3 and reduced ( P < 0 . 05 ) concentrations of IGFBP 2 . ^^^ Similarly , AL cattle had greater ( P < 0 . 05 ) serum concentrations of IGF 1 and IGFBP 3 and reduced ( P < 0 . 05 ) IGFBP 2 compared with 0 . 75 AL cattle . ^^^ In summary , treatment with bST increased growth rate and G : F and stimulated serum IGF 1 and IGFBP 3 while reducing IGFBP 2 . ^^^ Thus , limit feeding may reduce the effect of exogenous bST on growth rate by blunting bST induced increases in IGF 1 and IGFBP 3 and bST induced decreases in IGFBP 2 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , in 22 day old undernourished rats , IGF 1 and IGF receptor mRNA expression were increased in both the hypothalamus and cerebellum , while IGFBP 2 was decreased , but only in the hypothalamus . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To discover the mechanism of action of IGFBP 2 , we further tested the mitogenic potential of IGF 1 to counteract TNF alpha inhibition . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Moreover , the expression and production of IGF 1 and IGFBP 1 increased progressively from corpora lutea from the early to late luteal phases ( P < 0 . 05 ) , whereas the expression and production of IGFBP 2 , 4 and 5 were significantly higher in corpora lutea from the mid luteal phase ( P < 0 . 05 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The rhGH administration led to significant increases of IGF 1 , total IGFBP 3 , glycosylated IGFBP 3 ( 39 , 42 kDa ) and the 29 kDa fragment of IGFBP 3 and the decrease of IGFBP 2 during the trial ( P < 0 . 05 ) . ^^^ In spite of the significant changes of IGF 1 , IGFBP 2 , total IGFBP 3 and IGFBP 3 ( 29 kDa proteolytic fragment ) after rhGH administration to patients with malnutrition , maintained on parenteral nutrition , the increase of nitrogen balance was seen only in patients who decreased their IGFBP 1 and increased bioavailable IGF 1 as reflected by measurement of fIGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These results indicate that IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 5 are produced by equine granulosa cells and that insulin , FSH , and estradiol play a role in the regulation of steroidogenesis and the IGF system of equine granulosa cells . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGF 1 concentration was measured by radioimmunoassay and plasma IGFBP 3 and IGFBP 2 were estimated by Western ligand blotting . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : Birth weight ( BW ) , birth length ( BL ) , ponderal index , head circumference ( HC ) , and cord serum levels of albumin , prealbumin , retinol binding protein ( RBP ) , total and free IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and leptin were significantly lower , whereas levels of IGFBP 1 , IGFBP 2 , and IL 6 were significantly higher in preterm than in term neonates ( P < 0 . 05 ) . ^^^ Total and free IGF 1 , ALS , and leptin had significantly positive correlations , whereas IGFBP 2 had a significantly negative correlation , with BW and BL in preterm plus term neonates . ^^^ Forward stepwise multivariate regression analysis showed that gestational age ( GA ) , IGFBP 2 , leptin , and free IGF 1 are significant predictors of BW ; GA , IGFBP 2 , ALS , transferrin , and leptin are significant predictors of BL ; and GA and free IGF 1 are significant predictors of HC in preterm and term neonates . ^^^ CONCLUSIONS : Our results suggest that IGF 1 , IGF 2 , IGFBP 2 , ALS , and leptin play important roles in intrauterine growth . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 bioactivity is regulated in part by its six binding proteins ( IGFBP 1 to IGFBP 6 ) ; insulin inhibits the production of IGFBP 1 and perhaps IGFBP 2 . ^^^ As a result , chronically elevated fasting and postprandial insulin levels may lead to a decrease in circulating IGFBP 1 and IGFBP 2 concentrations and , consequently , an increase in IGF 1 bioavailability . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In this study the IGF 1 and IGFBP 2 content and the specific electrophoretic patterns of IGFBPs in samples of sera and pleural effusions of 25 patients with malignancy , infection and congestive heart failure were investigated . ^^^ This work demonstrates the existence of the IGF 1 / IGFBP system in pleural fluids from different etiologies and implies possible use of IGF 1 and IGFBP 2 as a potential marker of malignant effusions . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The effects of IGFBP 1 on cell survival in the presence of exogenously added IGF 1 were IGF 1 dependent , whereas IGFBP 2 displayed both IGF 1 dependent and IGF 1 independent effects . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
A period of 20 min of H / I that resulted in histopathology in cortex , striatum , and thalamus was correlated with induction of mRNA for IGF 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and glial fibrillary acidic protein ( GFAP ) by 4 days of recovery . ^^^ Increased IGF 1 mRNA was located within damaged regions and was surrounded by IGFBP 2 mRNA expression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Postmenopausal women had lower levels of plasma IGF 1 ( P < 0 . 001 ) and free IGF 1 ( P < 0 . 001 ) compared to premenopausal women , while plasma IGFBP 2 ( P < 0 . 05 ) and immunoreactive IGFBP 3 ( P < 0 . 001 ) were higher in postmenopausal women . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Concentrations of IGF 1 and IGFBP 2 in seminal plasma samples were determined by radioimmunoassay . ^^^ IGF 1 , IGFBP 2 , and IGFBP 5 were present in equine seminal plasma . ^^^ Concentrations of IGF 1 , IGF I / protein , total IGF 1 , IGFBP 2 , IGFBP 2 / protein , and total IGFBP 2 were not significantly different ( P > or = 0 . 13 ) in seminal plasma between stallions at either SR or SA . ^^^ At SR , stallions with higher seminal plasma IGF 1 had more total IGFBP 2 per ejaculate ( P < 0 . 01 ) , more morphologically normal sperm ( P = 0 . 05 ) , and higher first cycle pregnancy rates ( P = 0 . 02 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 2 , and 3 : do they have a role in detecting rhGH abuse in trained men . ^^^ METHODS : Morning serum growth hormone ( GH ) , IGF 1 , IGF binding protein ( BP ) 2 , IGFBP 3 , IGF I / IGFBP 2 , and IGFBP 3 / IGFBP 2 ratios were evaluated before , during ( 8th and 15th days ) , and at the end and after cessation ( +3 , +6 , +9 , +12 , and +15 d ) of a 3 wk treatment with different doses of rhGH ( 0 . 09 IU . kg BW ( 1 ) . d ( 1 ) for 6 or 3 d a week , i . e . , the A and B trials , respectively ) in seven well trained subjects not involved in competitive sports . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Under IGF 1 supplemented ( 10 nM ) culture conditions , IGF binding proteins ( IGFBPs ) 2 , 4 and 5 were secreted by the growth plate chondrocytes and IGFBP 2 protein and mRNA levels were decreased by the DXM treatment , whereas IGFBP 4 and 5 were not affected . ^^^ IGFBP 2 to 6 were expressed in the chondrocytes , IGFBP 1 was absent and both IGF 1 and IGF 2 , and the type 1 and type 2 IGF receptors were expressed . ^^^ Treatment with DXM ( 100 nM ) resulted in a 2 fold increase in mRNA levels of both IGFBP 5 and the type 1 IGF receptor , whereas IGFBP 2 mRNA levels decreased by 55 % , in concert with the decrease in protein level observed under IGF 1 supplemented culture conditions . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Adjustment for IGF 1 , IGF binding protein ( BP ) 1 , IGFBP 3 , smoking , or body mass index did not substantially alter the association , whereas adjustment for IGFBP 2 moderately attenuated the relationship . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : Intact IGFBP 1 , IGFBP 2 and IGFBP 6 inhibited in equimolar concentration the IGF 1 and IGF 2 stimulated DNA synthesis and cell proliferation , whereas the biological activity of IGFBP 3 was complex . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Only expression of IGF 1 , IGF 2 and IGFBP 2 could be detected in the growth plates of 7 week old normal mice . ^^^ DXM treatment of normal mice induced a significant 2 . 4 fold increase in the number of cells expressing IGF 1 mRNA , whereas IGF 2 and IGFBP 2 mRNA levels were not affected . ^^^ In hIGF 2 transgenic mice , IGF 1 mRNA levels were significantly increased , while endogenous IGF 2 and IGFBP 2 mRNAs were unaffected , compared to normal animals . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , dexamethasone treatment resulted in dramatic mucosal increases in IGFBP 2 , 3 , 4 , and 5 , paralleling the changing distribution of IGF 1 that we previously reported . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 2 ( IGFBP 2 ) separates hypertrophic and hyperplastic effects of growth hormone ( GH ) / IGF 1 excess on adrenocortical cells in vivo . ^^^ Our data show that IGFBP 2 potently separates hypertrophic and hyperplastic effects of GH / IGF 1 excess on adrenocortical cells . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Blood collection on days 1 , 15 and 30 for IGF 1 , IGFBP 3 , IGFBP 2 , bone alkaline phosphatase ( BAP , osteoblast marker ) , procollagen type 1 C terminal propeptide ( PICP , marker of type 1 collagen synthesis ) , procollagen type 3 N terminal propeptide ( P3NP , marker of soft tissue type 3 collagen synthesis ) and type 1 collagen telopeptide ( ICTP , marker of type 1 collagen breakdown ) . ^^^ At most time points , there were strong positive correlations ( 1 ) among IGF 1 , IGFBP 3 , BAP , PICP and P3NP ( P < 0 . 01 ) and ( 2 ) between IGFBP 2 and ICTP ( P < 0 . 01 ) . ^^^ Conversely , IGFBP 2 was negatively correlated with IGF 1 , IGFBP 3 , BAP , PICP and P3NP at most time points ( P < 0 . 01 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Explant cultures of bovine articular cartilage were used to assay release of exogenous IGF 1 and IGFBP 2 . ^^^ CONCLUSIONS : The Fn fs increased levels of IGFBPs in cultures of bovine articular chondrocytes and elicited release of IGFBP 2 and IGF 1 from articular cartilage . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We found transcriptional upregulation of both IGF 1 and IGF 2 in dystrophic muscle , however the possible beneficial effects of the growth factors appear offset by transcriptional upregulation of inhibitory IGF binding proteins and regulators ( IGFBP 2 , 4 , 6 and 7 ; and PRSS 11 [ IGFBP 5 protease ] ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Levels of IGFBP 2 , IGFBP 4 , IGF 1 and IGF 2 in media conditioned by 75 day fetal S 5 cultures were not influenced by late DEX . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The IGFBP 3 to IGFBP 2 ratio was altered in the presence of active disease in a manner that would reduce IGF 1 action . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of immunoreactive IGF 1 , free IGF 1 , IGFBP 2 and IGFBP 3 were measured before and after treatment and compared to controls matched for age , sex and puberty . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Furthermore , the equivalent mutations in the C terminus of rat IGFBP 2 ( C Term 2 ) also results in a significant reduction in IGF 1 binding , suggesting that the highly conserved Gly and Gln residues have a conserved IGF 1 binding function in all six IGFBPs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There was also a correlation between serum levels of IGF 1 and IGFBP 3 and an inverse correlation between the serum level of IGF 1 and serum levels of IGFBP 1 and IGFBP 2 in the ANS group . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
These data suggest that a plant based diet is associated with lower circulating levels of total IGF 1 and higher levels of IGFBP 1 and IGFBP 2 . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Concentrations of IGF 1 , insulin , glucose , and IGFBP 1 , IGFBP 2 , and IGFBP 3 in serum drawn at least 2 years before diagnosis ( mean times of 10 . 5 and 15 . 8 years for pre and postmenopausal cases , respectively ) were compared using conditional logistic regression analysis . ^^^ In premenopausal women , elevated serum IGF 1 and IGFBP 3 are associated with increased breast cancer risk , whereas elevated serum IGFBP 2 is inversely associated with risk of postmenopausal breast cancer . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of testosterone ; estrone ; estradiol ; dehydroepiandrosterone and dehydroepiandrosterone sulfate ; insulin like growth factor 1 ( IGF 1 ) ( total and free ) and its binding proteins IGFBP 1 , IGFBP 2 , and IGFBP 3 ; and leptin were measured . ^^^ Serum estradiol ; dehydroepiandrosterone sulfate ; total IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 ; and leptin showed no association . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum T and F IGF 1 and IGF 2 were determined using noncompetitive time resolved monoclonal immunofluorometric assays , IGFBP 1 was determined by an in house RIA , IGFBP 2 by a novel in house time resolved immunofluorometric assay , whereas IGFBP 3 by an immunoradiometric assay . ^^^ Specifically , mean T IGF 1 for patients group was 131 ( 61 ) , F IGF 1 was 0 . 47 ( 0 . 16 ) , T IGF 2 was 1056 ( 300 ) , F IGF 2 was 1 . 45 ( 0 . 54 ) , IGFBP 1 was 33 ( 14 ) , IGFBP 2 was 848 ( 377 ) , and finally IGFBP 3 was 3953 ( 1422 ) . ^^^ For controls , mean T IGF 1 was 146 ( 51 ) , F IGF 1 was 0 . 85 ( 0 . 43 ) , T IGF 2 was 939 ( 197 ) , F IGF 2 was 1 . 53 ( 0 . 53 ) , IGFBP 1 was 44 ( 24 ) , IGFBP 2 was 764 ( 316 ) and finally IGFBP 3 was 3721 ( 1017 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The components of the IGF 1 axis showed a similar pattern , with a peak value after exercise compared with baseline for IGF 1 ( P < 0 . 001 , males and females ) ; IGFBP 3 ( P < 0 . 001 , males and females ) ; acid labile subunit [ P < 0 . 001 , males ; not significant ( NS ) , females ] , and IGFBP 2 ( P < 0 . 05 , females ; NS , males ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , no significant effect of estrogen was seen on IGF 1 , IGFBP 2 or IGFBP 3 . ^^^ In conclusion , our data suggest that chronic rhIGF 1 administration increases IGF 1 and IGFBP 2 and decreases IGFBP 3 in women with anorexia nervosa . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In normal testes a clear expression of IGF 1 , IGF 2 , IGF IR , IGFBP 2 , IGFBP 4 and IGFBP 5 was found . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Compared with controls , immunoreactivity in the spinal motor neurons of patients with amyotrophic lateral sclerosis was 64 % higher for IGFBP 2 , 46 % higher for IGFBP 5 , and 33 % higher for IGFBP 6 , with upregulation of IGF 1 receptors . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , in previous studies we showed that in the skin of fasted rats IGF 1 as well as IGFBP 1 and IGFBP 2 expressions were not different , compared to control rat skin , although collagen content was significantly decreased . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : When compared with controls , diabetic patients ( n = 58 ) showed reduced ( P < 0 . 0005 ) levels of free and total IGFs , free plus dissociable IGF 1 and IGFBP 3 , whereas levels of IGFBP 1 , IGFBP 1 bound IGF 1 and IGFBP 2 were elevated . ^^^ When comparing diabetic patients with different degrees of retinopathy , IGFBP 2 and IGFBP 1 bound IGF 1 were the only parameters that differed significantly . ^^^ Patients with retinopathy ( non PDR as well as PDR ) had elevated IGFBP 2 ( P < 0 . 03 ) and reduced IGFBP 1 bound IGF 1 ( P < 0 . 03 ) , when compared with patients without retinopathy . ^^^ CONCLUSION : Our study gives no evidence of a direct role of circulating free IGF 1 in the development of PDR , whereas IGFBP 2 and IGFBP 1 bound IGF 1 showed a relationship with the degree of retinopathy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We sought to examine the relationships among maternal IGF 1 , IGF binding protein ( IGFBP ) 1 , and IGFBP 2 , with maternal and newborn anthropometry , in a cohort of 325 nondiabetic pregnant women of African origin . ^^^ Blood was collected for IGF 1 , IGFBP 1 , and IGFBP 2 at 9 , 25 , and 35 wk gestation and in cord blood at delivery . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To this end , we evaluated the behavior not only of IGF 1 but also of IGF 2 , IGF binding protein ( IGFBP ) 3 , and acid labile subunit , the subunits that constitute the main IGF complex in circulation ( 150 kDa complex ) , as well as of IGFBP 1 and IGFBP 2 , which are negatively regulated by GH and , like IGFBP 3 , able to influence the bioavailability of the IGFs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : Age was a strong determinant of both IGF 1 and IGFBP levels ; women aged 65 70 years had significantly lower IGF 1 and IGFBP 3 concentrations and significantly higher IGFBP 1 and IGFBP 2 concentrations than women aged 20 24 years . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Measuring IGF 1 , IGFBP 2 and IGFBP 3 from dried blood spots on filter paper is not only practical but also reliable . ^^^ OBJECTIVE : The aim of this study is to adapt measurements of IGF 1 , IGFBP 2 and IGFBP 3 to dried blood filter disk assays . ^^^ Recovery of IGF 1 , IGFBP 2 and IGFBP 3 in experiments using recombinant standards mixed with whole blood was well correlated with the recovery of the plasma fraction on the filter paper . ^^^ CONCLUSIONS : Taken together , these data clearly demonstrate that such a filter paper assay is a reliable procedure to monitor changes of IGF 1 , IGFBP 2 and IGFBP 3 content in blood . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We report that these genes fall into two distinct groups with respect to expression pattern , with IGFBP 2 displaying broad overlap of mRNA expression with IGFR and IGF 1 during early development , whereas the expression profile of IGFBP 5 most closely resembled that of IGF 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the growth plates , both IGF 1 and IGF 2 , as well as IGFBP 2 mRNAs were detected , mainly in the proliferative and prehypertrophic zones . ^^^ DXM treatment significantly decreased the number of chondrocytes expressing IGF 1 , whereas the number of chondrocytes expressing IGF 2 and IGFBP 2 were not affected . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Low serum IGFBP 2 concentrations are a powerful indicator for overall good physical functional status , probably inversely reflecting the integrated sum of nutrition and the biological effects of growth hormone , IGF 1 and insulin . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
For IGF 1 , the adjusted OR ( 95 % CI ) of the top vs . bottom quartile was 1 . 1 ( 0 . 6 ; 2 . 1 ) ; for IGFBP 1 it was 0 . 7 ( 0 . 3 ; 1 . 3 ) ; for IGFBP 2 , 1 . 1 ( 0 . 5 ; 2 . 4 ) ; for IGFBP 3 , 1 . 6 ( 0 . 7 ; 3 . 5 ) , for C peptide , 1 . 3 ( 0 . 7 ; 2 . 7 ) and for IGF I / IGFBP 3 ratio , 1 . 0 ( 0 . 5 ; 1 . 8 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 , IGFBP 5 , and nonglycosylated IGFBP 3 were shown to significantly enhance binding of IGF 1 to VN , whereas IGFBP 2 and glycosylated IGFBP 3 had a smaller effect . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have previously reported that the galactopoietic effect of LDPP is associated with an increase in circulating IGF 1 , which seems to occur independently of changes in concentrations of GH , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
After binding with IGFBP 1 , IGFBP 2 and IGFBP 6 , IGF 1 passes through epithelium and reaches the target cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There were no differences in serum IGF 1 or IGFBP 2 , 3 , 4 , and 5 concentrations at 48 h between groups 1 and 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Although IGFBP 2 is one of the most commonly overexpressed genes in hormone refractory prostate cancer , the functional significance of changes in IGF 1 signaling during AI progression remains poorly defined . ^^^ Increased IGFBP 2 levels after androgen ablation may represent an adaptive response that helps potentiate IGF 1 mediated survival and mitogenesis and promote androgen independent tumor growth . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Type 2 diabetes were associated with a decline of IGF 1 ( p < 0 . 05 ) and IGFBP 2 ( p < 0 . 05 ) serum levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
LF mice had significantly lower levels of serum insulin , tumor IGF 1 mRNA expression , and tumor IGFBP 2 immunostaining and higher levels of serum IGFBP 1 ( by Western ligand blot ) relative to the HF mice . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Changes of serum growth factors ( IGF 1 , 2 and IGFBP 2 , 3 ) prior to and after stem cell transplantation in children with acute leukemia . ^^^ In 51 patients ( AML n=27 ; ALL n=24 ; mean age 11 . 2+ / 4 . 8 years ) , serum parameters ( IGF 1 , 2 , IGFBP 2 , 3 ) were investigated up to 18 months after HSCT by RIA . ^^^ Children with AML showed significantly higher IGFBP 2 ( P=0 . 04 ) and significantly lower IGF 1 ( P=0 . 03 ) and IGFBP 3 ( P=0 . 05 ) levels than children with ALL at day 100 after HSCT . ^^^ Increased IGFBP 2 and decreased IGF 1 and IGFBP 3 may be associated with the increased proliferation rate of transplanted bone marrow . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
When the effects of individual IGFBPs were tested , IGFBP 2 and IGFBP 4 were found to inhibit IGF 1 stimulated DNA synthesis and migration . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 6 were examined by specific assays . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The significantly reduced levels of IGF 1 ( p < 0 . 001 ) , IGF 2 ( p=0 . 017 ) and IGFBP 3 and the increased presence of IGFBP 2 were found in patients with echinococcosis . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels were significantly reduced , but by different degrees , in both mouse lines , but IGFBP 1 and 4 levels were reduced and IGFBP 2 levels increased in GHR / mice but unaltered in GHA mice . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The present study was undertaken to evaluate the changes over time in mRNA and peptide expression of IGF 1 and IGFBP 2 in the MRL / lpr mouse . ^^^ Using in situ hybridization and immunocytochemistry techniques , the expression of IGF 1 and IGFBP 2 in MRL / lpr mouse was examined and compared to their congenic normal MRL ++ mouse counterparts from nine to 24 weeks of age . ^^^ In the MRL ++ and MRL / lpr mouse kidneys , IGF 1 and IGFBP 2 mRNA expression was limited to the cortical and medullary collecting ducts , while their immunoreactivity ( IR ) was localized to the cortical and medullary collecting ducts , loop of Henle , glomeruli and proximal tubules . ^^^ Over time , and with progression of disease , the MRL / lpr mice displayed a significant increase in IGF 1 IR and a modest increase in IGFBP 2 IR within the outer cortical glomeruli , which was associated with a significant increase in glomerulosclerosis and glomerular cell proliferation and with a significant decrease in renal function . ^^^ In conclusion , this overexpression of IGF 1 and IGFBP 2 within the glomeruli of the MRL / lpr mouse kidney supports their potential role in the alterations in renal function and morphology that accompany lupus nephritis . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Overall , GH treatment increased plasma GH , IGF 1 ( both P < 0 . 001 ) , and IGFBP 3 ( P < 0 . 02 ) ; however , GH treatment increased total IGF 1 , free IGF 1 , and IGFBP 3 , and decreased IGFBP 2 mainly at the high feeding level ( GH 10 feeding level interaction ; P < 0 . 02 , 0 . 01 , 0 . 03 , and 0 . 10 , respectively ) . ^^^ The high feeding level increased insulin , free and total IGF 1 , and IGFBP 3 ( all P < 0 . 001 ) , but decreased GH and IGFBP 2 ( both P < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
METHODS : In 50 ( 39 males , 11 females ) histologically confirmed and TNM graded patients with HCC who had not received adjuvant chemotherapy , the basal serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and alpha fetoprotein ( AFP ) were measured . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The transcripts of IGF 1 , IGF 2 , IGF receptor ( IGF R ) , two IGF binding proteins ( IGFBP 2 and IGFBP 5 ) , GH receptor ( GH R ) and insulin receptor ( 1 R ) were measured by RT PCR at 4 , 8 , 12 and 16 weeks of age in the ovaries of ad libitum fed and feed restricted hens . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of ST , IGF 1 , IGFBP 2 , and IGFBP 3 were determined . ^^^ Correlations between average daily gain , ST , IGF 1 , IGFBP 2 , and IGFBP 3 were determined . ^^^ Concentrations of IGFBP 2 were negatively correlated ( P < 0 . 01 ) with IGF 1 and positively correlated ( P < 0 . 01 ) with IGFBP 3 . ^^^ In conclusion , serum concentrations of ST , IGF 1 , IGFBP 2 , and IGFBP 3 differed between male and fe male calves . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We measured height , weight , and lower leg length ( LLL ; n = 10 ) and markers of bone formation [ bone alkaline phosphatase ( BALP ) and C terminal propeptide of type 1 collagen ( P1CP ) ] , bone resorption [ C terminal telopeptide of type 1 collagen ( 1CTP ) ] , soft tissue collagen turnover [ N terminal propeptide of type 3 procollagen ( P3NP ) ] , and the GH axis [ IGF 1 and its binding proteins ( IGFBP 3 and IGFBP 2 ) ] before and after each course ( n = 25 ) and on completion of treatment ( n = 12 ) . ^^^ At diagnosis , BALP , PICP , and IGF 1 SD score were low compared with age and sex matched reference groups ( p < 0 . 001 , p < 0 . 001 , and p < 0 . 002 , respectively ) and IGFBP 2 was elevated ( p < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , in the presence of IGFs , IGFBP 2 binding to ECM was markedly enhanced , with IGF 2 being more effective than IGF 1 . ^^^ There was no difference between soluble and ECM associated IGFBP 2 in affinity for IGF 1 and IGF 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Determination of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in serum and plasma : comparisons using the Bland Altman method . ^^^ Blood was obtained from 47 healthy volunteers ( age 21 72 years ) in serum , heparin plasma and EDTA plasma , and IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 measured , and results compared . ^^^ Mean values for IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 determined in all three media were generally comparable ; correlations were generally strong and significant ( P < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Risk was unrelated to levels of IGF 1 , IGFBP 2 and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In 1999 calves , none of the IGFBP were correlated with serum IGF 1 , although IGFBP 2 was negatively correlated with several measures of body weight . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have assessed 46 adult STS specimens of low , intermediate and high pathological grade of malignancy for the immunohistochemical expression of IGFBP 2 , IGF 1 , IGF 2 , IGF 1 receptor alpha and beta ( IGF1Ralpha / beta ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
OBJECTIVE : To assess the effect of pegvisomant induced serum insulin like growth factor 1 ( IGF 1 ) normalization on IGF binding proteins 1 , 2 , 3 ( IGFBP 1 , IGFBP 2 and IGFBP 3 ) , total , non bound ( 45 kDa ) and 150 kDa ternary complex associated IGFBP 3 , and in vivo IGFBP 3 proteolysis in patients with active acromegaly . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Assays of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were performed on cases and 2 controls matched on age , recruitment center and calendar time . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In colostrum fed calves , DEXA decreased ( P < 0 . 05 ) mRNA levels of IGF 1 ( esophagus , fundus , duodenum , and ileum ) , IGF 2 ( fundus ) , IGFBP 2 ( fundus ) , IGFBP 3 ( fundus ) , IGF1R ( esophagus , ileum , and colon ) , IGF2R ( fundus ) , GHR ( fundus ) , and InsR ( esophagus , fundus ) , but in formula fed calves DEXA increased mRNA levels of IGF 1 ( esophagus , rumen , jejunum , and colon ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
This suggests that IGFBP 2 and 5 may limit IGF 1 availability thus decreasing survival signaling . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Feeding a high diet or treating with rbST increased serum IGF 1 and decreased serum IGFBP 2 . ^^^ The observed changes in serum IGF 1 and IGFBP 2 were reflected in their respective liver mRNA amounts . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Assays of IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , and free and total PSA were performed . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The results suggest that the increased expression of IGF 1 and 2 mRNAs in preovulatory follicles , along with the decrease in IGFBP 2 concentrations , could increase the bioavailability of intrafollicular IGF in large follicles during the breeding season , and support our hypothesis that intrafollicular IGF bioavailability must exceed a threshold level before ovulation can occur . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Synthesis and characterization of biotinylated forms of insulin like growth factor 1 : topographical evaluation of the IGF 1 / IGFBP 2 AND IGFBP 3 interface . ^^^ To identify key domains of IGF 1 involved in the interaction with IGFBP 2 and IGFBP 3 , we employed IGF 1 selectively biotinylated on residues Gly 1 , Lys 27 , Lys 65 , and Lys 68 . ^^^ All monobiotinylated species of IGF 1 exhibited high affinity ( approximately 0 . 1 0 . 2 nM ) for IGFBP 2 and IGFBP 3 in solid phase binding assays . ^^^ When taken together , these results suggest that , once bound to IGFBP 2 or IGFBP 3 , the biotin moieties of N ( alpha ) ( Gly1b ) IGF 1 and N ( epsilon ) ( Lys27b ) IGF 1 are inaccessible to NeutrAvidin peroxidase , the secondary binding component . ^^^ In BIAcore analysis , IGFBP 2 and IGFBP 3 bound only to the N ( epsilon ) ( Lys65 / 68b ) IGF 1 coated flowcell of a biosensor chip , confirming the inaccessibility of Gly 1 and Lys 27 when IGF 1 is bound to IGFBP 2 and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To clarify the localization and influence of the hIGF 2 transgene on different components of the GH IGF axis in the brain , we studied the expression pattern of the hIGF 2 transgene , endogenous IGF 1 and IGF 2 , and IGFBP 2 , 3 and 5 in the brain of prepubertal 4 week old mice , using nonradioactive in situ hybridization . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We determined serum levels of insulin like growth factors ( IGF 1 , IGF 2 ) and binding proteins ( IGFBP 2 and IGFBP 3 ) at diagnosis ( 1 ) , during ( 2 ) and after therapy ( 3 ) in 28 children treated for ALL . ^^^ RESULTS : 1 ) Serum absolute levels of IGF 1 , IGF 2 , IGFBP 3 rose and IGFBP 2 decreased during analysis . 2 ) Mean values of IGF 1 SDS were similar at diagnosis , during and after treatment ( 1 . 78+ / 0 . 9 vs . 1 . 86+ / 1 . 36 vs . 1 . 8+ / 0 . 83 ) . ^^^ CONCLUSIONS : Decreased serum IGF 1 and elevated IGFBP 2 were observed at diagnosis and during treatment for ALL suggesting that especially IGFBP 2 may be related to the proliferation of lymphoblasts . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IGF 1 concentrations were measured by RIA ; IGFBP 1 and IGFBP 2 by IRMA and IL 6 concentrations using quantitative ELISA immunoassays . ^^^ RESULTS : IL 6 and IGF 1 , IGFBP 1 and IGFBP 2 levels did not differ statistically significant between diabetic patients and controls . ^^^ IGF 1 positively correlated with age and growth in both groups and with IGFBP 2 in diabetic patients . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Endometrial mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 increased in bST C , but not in bST P cows . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There was no association between IGF 1 , IGF 2 , IGFBP 2 , or IGFBP 3 and risk of ER negative breast cancer . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP profiles correlated with the amount of IGF present : patients with lower IGF 1 concentrations expressed decreased IGFBP 3 and elevated IGFBP 2 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Stimulation of myofibroblastic Mller cells by IGF 1 and 2 , but not PDGF , further increased message abundance and production of IGFBP 2 , 4 , 5 , and 6 , but not IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Total and free IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 protease were similar between groups ( all P > . 5 ) , whereas , in LIPO , IGFBP 1 and IGFBP 2 were reduced ( 36 % , P < . 05 and 50 % , P < . 01 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Neither 100 ng / ml of IGF 1 nor IGF 2 had an effect ( P > 0 . 10 ) on IGFBP 2 , 3 , 4 , or 5 mRNA levels in small follicle ( 1 5 mm ; Experiment 1 ) granulosa cells . ^^^ In large follicle ( > 7 . 9 mm ; Experiment 2 ) granulosa cells , 100 ng / ml of IGF 1 increased ( P < 0 . 05 ) IGFBP 2 mRNA levels above controls and 3 ng / ml of IGF 1 ; 100 ng / ml of IGF 1 also decreased ( P < 0 . 10 ) IGFBP 5 mRNA levels compared to 3 ng / ml of IGF 1 or FSH or 100 ng / ml leptin , while 100 ng / ml of IGF 2 had no effect ( P > 0 . 10 ) on IGFBP 2 , 3 , 4 , and 5 mRNA levels ( Experiment 2 ) . ^^^ In theca cells , IGF 2 decreased ( P < 0 . 05 ) IGFBP 2 mRNA levels , but had no effect on IGFBP 3 or 4 mRNA expression ( Exp . 3 ) ; IGF 1 did not affect ( P > 0 . 10 ) thecal IGFBP 2 , 3 or 4 mRNA levels . ^^^ Ligand blotting revealed that both IGF 1 and 2 increased IGFBP 2 and 5 ( protein ) and had no effect on IGFBP 3 ( protein ) , whereas IGF 1 ( but not IGF 2 ) increased IGFBP 4 ( protein ) , suggesting IGFBP 2 , 4 , and 5 are post transcriptionally regulated . ^^^ These results suggest that expression of IGFBP 2 , 3 , 4 , and 5 mRNA by granulosa and theca cells are differentially regulated by IGF 1 and 2 , therefore discretely modulating the amount of bio available IGFs to these cells depending upon the specific hormonal milieu . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In conclusion , in undernourished fetuses the increased beta cell mass can be related to the stimulation of replicative beta cell response due to locally increased pancreatic IGF 1 mRNA ; this effect is perhaps potentiated or favored by the enhanced islet IGF 1 receptor content and pancreatic IGFBP 2 gene expression . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
All four hormones increased gene and / or protein expression of PSA , IGF IR , IGF 1 , and IGFBP 2 and decreased that of AR , ERbeta , IGF 2 , and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We studied by real time reverse transcription PCR , whether there are differences in the abundance of mRNA coding for IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGF 1R , IGF 2R , GHR , and InsR in compartmentalized layers ( fractions ) of jejunum and ileum of 5 d old calves fed colostrum . ^^^ In ileum , mRNA levels , expressed relative to housekeeping genes , differed ( P < 0 . 05 ) between fractions for IGF 1 ( LP > villi , crypts ) , IGF 2 , and IGFBP 3 ( villi > crypts , LP ) , GHR and InsR ( crypts > LP ) , IGFBP 2 ( crypts > villi , LP ) , and total RNA levels were greater ( P < 0 . 05 ) in LP and crypt than in villus fractions . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Secretion of IGF 1 , IGF 2 , and IGFBP 2 by PBMCs from healthy adult donors was determined by radioimmunoassays ( RIAs ) . ^^^ The PBMC proliferative response after stimulation with anti CD 3 mAb and exposure to increasing concentrations of IGF 1 , IGF 2 , IGFBP 2 , and anti IGFBP 2 were determined by bromodeoxyuridine enzyme linked immunosorbent assay . ^^^ The stimulatory activity of IGFBP 2 ( 1 10 ng / ml ) on anti CD 3 mAb activated PBMCs was similar to that of IGF 1 and IGF 2 ( 1 100 ng / ml ) , with the mean increase in PBMC proliferative response ranging between 150 % and 160 % for IGFBP 2 ( p = 0 . 03 ) , 150 % and 170 % for IGF 1 ( p < 0 . 01 ) , 133 % 161 % for IGF 2 ( p < 0 . 01 ) , and 157 % and 175 % for IGF 1 + IGF 2 ( p < 0 . 01 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Free IGF 1 , total IGF 2 , IGFBP 3 , and GH binding protein were lower , whereas IGFBP 1 , IGFBP 1 bound IGF 1 , and IGFBP 2 were elevated compared with control values . ^^^ Patients with detectable C peptide ( > or =100 pmol / liter ) had higher levels of total IGF 1 , free IGF 1 , and total IGF 2 and lower levels of IGFBP 1 and IGFBP 2 than those with an undetectable C peptide level despite having identical average HbA1c . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We analyzed plasma ghrelin levels and their correlation with plasma levels of leptin , insulin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) , insulin like growth factor binding protein 2 ( IGFBP 2 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and integrated concentration of growth hormone ( ICGH ) before and after dietary intervention . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
However , ES did not significantly alter the transcription of igf 1 , igfbp 2 , igfbp 5 and igfbp 6 genes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
On the other hand , exogenous IGF 1 induced 8 11 % ( p < 0 . 05 ) increases in the proliferation , but cyclosporin A induced 30 80 % ( p < 0 . 05 0 . 01 ) reductions in the mRNA expression levels for endogenous IGF 1 , IGFR 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Targeted knock down of IGFBP 2 by antisense morpholino modified oligonucleotides resulted in delayed development , reduced body growth , reduced IGF 1 mRNA levels , and disruptions to cardiovascular development , including reduced blood cell number , reduced blood circulation , cardiac dysfunction , and brain ventricle edema . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 , and IGFBP 3 were measured in 162 children with acute lymphoblastic leukemia treated by the Berlin Frankfurt Munster Study Group . ^^^ At diagnosis we found elevated IGFBP 2 , low IGFBP 3 , low IGF 1 , and low normal IGF 2 , but normal IGFBP 1 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the control group , total ghrelin correlated positively with IGFBP 2 ( p < 0 . 05 ) and negatively with active ghrelin ( p=0 . 05 ) , BMI ( p < 0 . 05 ) , WHR ( p < 0 . 05 ) , insulin ( p=0 . 01 ) and IGF 1 ( p=0 . 05 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGF 1 in the HP group were higher than in the LP group ( P < 0 . 05 ) , but plasma 34 kDa IGFBP 2 was lower in the HP than the LP group ( P < 0 . 05 ) . ^^^ Although the response of plasma IGF 1 concentration to GHRP 2 administration was increased in the HP group ( P < 0 . 05 ) , there was no apparent effect of GHRP 2 treatment on plasma 38 43 kDa IGFBP 3 and 34 kDa IGFBP 2 at days 2 and 6 of treatment . ^^^ In conclusion , it is proposed that the 34 kDa IGFBP 2 is sensitive to dietary protein level and may play an important role in the regulation of circulating IGF 1 in ruminant . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Combined treatment caused a higher increase in IGFBP 1 and larger suppression of total and free IGF 1 , IGF 2 , and IGFBP 3 ( P < 0 . 01 for all ) , but less suppression of IGFBP 2 ( P < 0 . 05 ) compared with tamoxifen monotherapy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Lack of synergy between estrogen and progesterone on local IGF 1 , IGFBP 3 and IGFBP 2 secretion by both hormone dependent and hormone independent breast cancer explants in vitro . ^^^ The aim of the present study was to investigate direct effects of estrogen ( E 2 ) or progesterone ( P 4 ) given separately vs . estrogen+progesterone on local IGF 1 , IGFBP 3 and IGFBP 2 secretion . ^^^ In hormone dependent and hormone independent tissues , treatment with estrogen+progesterone increased IGF 1 and IGFBP 2 secretion with concomitant decrease in IGFBP 3 , in the same manner as E 2 or P 4 given alone . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
A total of 283 SNP were discovered in 31 897 base pairs ( bp ) from 12 genes of the growth hormone ( GH ) , growth hormone receptor ( GHR ) , ghrelin , growth hormone secretagogue receptor ( GHSR ) , insulin like growth factor 1 and 2 ( IGF 1 and 2 ) , insulin like growth factor binding protein 2 ( IGFBP 2 ) , insulin , leptin receptor ( LEPR ) , pituitary specific transcription factor 1 ( PIT 1 ) , somatostatin ( SS ) , thyroid stimulating hormone beta subunit ( TSH beta ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
METHODS : Preoperative sera from 141 patients undergoing RRP were analyzed for IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 . ^^^ Average levels of IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 were 156 . 5 ng / ml , 48 . 1 ng / ml , 396 . 8 ng / ml and 3303 . 7 ng / ml , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the present study , the in vivo effects of estradiol 17beta ( E 2 ) and growth hormone ( GH ) exposure on IGF 1 , IGF 2 , IGF1R , and IGFBP 2 gene expression in sea bream ovary were monitored by RT PCR during prereproductive and reproductive periods . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IL 1beta , IL 6 , TNF alpha , IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , TSH , fT 3 and fT 4 were measured using standard commercial kits . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Changes in IGFBP 2 , free IGF 1 , and total IGF 1 responses were minimal and did not differ significantly from those during the 4 h fast . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Evidence against a direct effect of leptin on insulin like growth factor 1 ( IGF 1 ) , IGFBP 2 and IGF 1 receptor expression in human SK N MC neuroepithelioma cells . ^^^ SK N MC cells were chosen for further investigation since they express IGF 1 , IGF 1 receptor and IGFBP 2 mRNA and secrete IGF 1 and IGFBP 2 protein . ^^^ Secondly and most importantly , when SK N MC cells were cultured in the presence of leptin , neither IGF 1 , nor IGF 1 receptor or IGFBP 2 mRNA expression was different than in the absence of leptin . ^^^ In addition , an increase in leptin did not alter secretion of immunoreactive IGF 1 or IGFBP 2 protein into cell culture medium . ^^^ In conclusion , ( 1 ) human SK N MC neuroepithelioma cells express ob and leptin receptor mRNA and secrete leptin . ( 2 ) Added exogenous leptin does not affect IGF 1 , IGFBP 2 or IGF 1 receptor mRNA expression and IGF 1 and IGFBP 2 secretion by SK N MC cells in vitro under the conditions studied . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 5 protein expression was evaluated by Western blot analysis . mRNA expression in microarray and real time RT PCR showed similar tendencies : IGF 1 , IGF R 1 , IGF R 2 , IR A , and IGFBP 2 were not different in both groups . ^^^ Constantly expressed genes ( e . g . , IGF 1 , IGF R 1 , IR A , and IGFBP 2 ) may reflect an involvement in spermatogenesis , but may also play a major role in tumor growth as their expression is not downregulated despite the lack of spermatogenesis in tumor tissue . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We have previously shown that IGFBP 2 binds and localizes IGF 1 to the pericellular matrix and have provided some evidence suggesting that the heparin binding domain ( HBD ) or the arginine glycine aspartic acid ( RGD ) integrin binding motif may be involved in these interactions . ^^^ IGF 1 ( 100 ng / ml ) increased incorporation of 3H thymidine in neuroblastoma SK N SHEP cells by approximately 30 % , an effect blunted by exogenously added native or either mutant IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 mRNA and protein levels were observed under basal conditions in both cell types , with no significant regulation by IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Measures at baseline , 6 and 12 months included body weight , height , body fat , lean mass , body fat % , and waist circumference , as well as fasting glucose , insulin , insulin resistance , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In addition , IGF 1 mRNA was present in the cornea , IGFBP 1 mRNA was observed in the cornea and iris , and IGFBP 5 and 6 mRNAs were identified in the ciliary body , iris , and cornea . mRNAs for IGFBP 2 , 3 , and 4 were not found in the eyes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There were no differences in plasma concentrations of total IGF 2 , free IGF 1 and 2 , IGFBP 3 , GHBP and ghrelin , whereas a small difference was found for IGFBP 2 ( 302+ / 97 vs . 263+ / 66 microg / L ; LNP vs . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Overexpression of PAPP A led to degradation of the IGFBP 2 produced by C2C12 myoblasts and increased free IGF 1 concentrations without affecting total IGF 1 concentrations . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The IGF binding proteins , IGFBP 2 and 3 , have been shown to inhibit binding of IGF 1 and 2 to bovine luteal cells and decrease progesterone production . ^^^ We conclude that the genetic capacity for increased IGFBP 2 production in the early stages of natural luteolysis in the mare may act to sequester IGF 1 in the CL , assisting in inhibition of progesterone production . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
There was no correlation of plasma IGFBP 4 level with age , sex , immunophenotype , or ALL risk group , and there was no correlation of IGFBP 4 level with plasma IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 levels . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We assessed the association of prediagnostic plasma levels of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and subsequent occurrence of prostate cancer in a case control study nested in the SU . ^^^ Frozen baseline plasma samples were used to measure IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 . ^^^ After controlling for the intervention group in the trial and the other IGF axis variables , the odds ratios and 95 % confidence interval ( 95 % CI ) comparing the upper quartile to the baseline quartile were 1 . 83 ( 95 % CI , 0 . 85 3 . 95 ) , 1 . 05 ( 95 % CI , 0 . 35 3 . 18 ) , 0 . 79 ( 95 % CI , 0 . 39 1 . 58 ) , and 0 . 42 ( 95 % CI , 0 . 12 1 . 52 ) for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , respectively . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Role of IGFBP 2 , IGF 1 and IGF 2 in regulating long bone growth . ^^^ IGF 1 and IGFBP 2 are co expressed in dynamic fashions in the developing long bones of the chick wing , and we have found that IGF 2 is present in the cartilage model and surrounding perichondrium , proliferative and hypertrophic chondrocytes and developing periosteum . ^^^ To gain insight into endogenous roles of IGF 1 , IGF 2 and IGFBP 2 in long bone development , we have overexpressed IGFBP 2 in the developing skeletal elements of the embryonic chick wing in vivo , using an RCAS retroviral vector . ^^^ We also have found that IGFBP 2 blocks the ability of IGF 1 and IGF 2 to promote proliferation and matrix synthesis by wing chondrocytes in vitro . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Total ghrelin in CRF correlated positively with active ghrelin ( p < 0 . 001 ) , GH ( p < 0 . 05 ) , IGF 1 ( p < 0 . 05 ) , free IGF 1 ( p = 0 . 0001 ) , IGFBP 3 ( p < 0 . 01 ) , IGFBP 2 and 6 ( p < 0 . 05 ) . ^^^ Active ghrelin in CRF correlated positively with IGF 1 ( p < 0 . 001 ) , free IGF 1 ( p < 0 . 005 ) , IGFBP 2 ( p < 0 . 05 ) and IGFBP 3 ( p < 0 . 05 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Relationship between insulin like growth factors ( IGF 1 and IGF 2 ) , IGF binding proteins ( IGFBP 3 , IGFBP 2 ) , leptin and anthropometric parameters ( height , body mass index ) during antileukaemic treatment in children . ^^^ PURPOSE : The aim of the study was to estimate the anthropometric parameters and their relationship to serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin before and during intensive antineoplastic treatment for acute lymphoblastic leukaemia in children . ^^^ MATERIAL AND METHODS : In 46 children in median age 6 . 6 years ( range from 1 . 6 to 16 ) we evaluated at the time of diagnosis , after protocol 1 and after intensive treatment , height , body mass index ( BMI ) and IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin . ^^^ IGF 1 SDS was low and similar at each point , IGF 2 SDS and IGFBP 3 SDS values augmented progressively and IGFBP 2 SDS was significantly elevated before treatment and lowered ( but not normalized ) during the therapy . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
DHT and testosterone , but not DHEA or E 2 , differentially modulate IGF 1 , IGFBP 2 , and IGFBP 3 in human prostatic stromal cells . ^^^ DHT and T each increased IGF 1 ( 7 fold ) and decreased IGFBP 3 ( 2 fold ) mRNA expression and protein secretion in a dose and time dependent manner and increased IGFBP 2 ( 2 fold ) mRNA in a dose and time dependent manner . ^^^ Flutamide abolished the DHT modulated increases in IGF 1 and IGFBP 2 , suggesting that the influences of DHT and T on these measures were androgen receptor mediated . ^^^ The effects of DHT on IGF 1 , IGFBP 2 , and IGFBP 3 were more pronounced in stromal cultures that did not express desmin . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IL 1beta , IL 6 , TNFalpha , insulin and C peptide were measured . ^^^ Serum concentrations of IGF 1 and IGF 2 were significantly lower and insulin and C peptide levels significantly increased in CF compared with healthy controls whereas IGFBP 3 serum concentrations were similar , with comparable IGF I / IGFBP 3 and decreased IGF I / IGFBP 2 and IGF II / IGFBP 2 molar ratios . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Recombinant human IGFBP 2 significantly attenuated IGF 1 stimulated MCF 7 cell proliferation with coaddition of 20 or 100 nM IGFBP 2 ( 50 or 80 % inhibition , respectively ) . ^^^ We previously identified IGF 1 contact sites both upstream and downstream of the CWCV motif ( residues 247 250 ) in human IGFBP 2 ( J Biol Chem 276 : 2880 2889 , 2001 ) . ^^^ Based on competition binding analysis , deletion of residues 249 to 289 caused an approximately 20 fold decrease in IGF 1 binding affinity ( IGFBP 2 EC 50 = 0 . 35 nM and IGFBP 2 ( 1 248 ) = 7 nM ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In contrast , during the study period total and free IGF 2 decreased ( P < 0 . 05 ) , whereas total IGF 1 , IGFBP 1 and IGFBP 2 increased ( P < 0 . 001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In a rat osteoblast cell culture that we studied , IGF 2 and IGFBP 2 were the most abundant peptides of the IGF system released in cell medium , IGFBP 5 was abundantly expressed but bound to cell surface and cell matrix and IGF 1 and IGFBP 3 were found at very low concentrations . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Before treatment , in women and men , IGF 1 was directly related to IGFBP 3 ( P < 0 . 001 and P < 0 . 0001 ) and IGFBP 1 to IGFBP 2 ( P = 0 . 0001 ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Administration of anastrozole to boars reduced the percentage increase in serum concentrations of IGF 1 and relative amounts of AP IGFBP 2 and 5 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Hence , our objective is to examine relationships between IGF 1 levels ; body mass index ( BMI ) ; fasting insulin level ; IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 levels ; and an array of traditional cardiovascular risk factors . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : In fully adjusted models ( controlling for age , sex , socioeconomic factors , lifestyle , and body mass index ) , childhood height and its components were not associated with adult circulating IGF 1 , IGF 2 , or IGFBP 2 levels . ^^^ Height in adulthood was not associated with IGF 1 , IGF 2 , or IGFBP 3 and was inversely associated with IGFBP 2 ( P = 0 . 05 ) after additionally controlling for childhood height . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In 27 children with AML ( mean age 13 . 6+ / 5 . 3 years ; patients in remission n=15 with relapse n=12 ) serum parameters of IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 were analyzed up to 18 months after HSCT by RIA . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
In the present study , circulating IGF 1 , IGF 2 and their binding proteins ( IGFBP 3 , IGFBP 2 and IGFBP 1 ) were determined in 92 adult patients with gastrointestinal inflammation ( Crohn ' s disease , colitis ulcerosa , gastritis , duodenitis errosiva , gastrointestinal candidiasis , and rotaviral and adenoviral enteritis ) . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
RESULTS : CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell , IGF 1 antibodies , or exogenous IGF 1 and IGFBP 2 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Plasma IGFBP 2 content was significantly reduced in prenatal T treated newborns , but by 4 months these females had significantly higher circulating IGF 1 and IGFBP 3 concentrations and faster growth rates than control females . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The effects of IGF 1 on bovine follicle development and IGFBP 2 expression are dose and stage dependent . ^^^ This study aimed to determine the effect of insulin like growth factor 1 ( IGF 1 ) on early antral bovine follicular development , and the expression of insulin like growth factor binding protein 2 ( IGFBP 2 ) . ^^^ Oocyte health of the largest follicles ( 281 380 microm ) was significantly improved by the addition of IGF 1 to the culture medium . mRNA expression of IGFBP 2 was decreased in the granulosa cells of follicles , size range 216 280 microm , cultured with a high dose of IGF 1 ( P < 0 . 05 ) . ^^^ Granulosa cells ( P < 0 . 05 ) and oocytes ( P < 0 . 01 ) of the largest follicles ( 281 380 microm ) showed a decrease in IGFBP 2 expression ( protein ) when cultured in the control and low IGF 1 treatment groups . ^^^ Furthermore , the inverse relationship between the level of IGF 1 stimulation and IGFBP 2 expression suggests a local regulatory system modulating IGF 1 availability . . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
The results indicate an increase in IGF 1 ( P < 0 . 01 ) and a decrease in IGFBP 2 ( P < 0 . 05 ) mRNA expression in slow muscle of the stimulated group compared with the control group . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
We report on a 10 yr old Caucasian girl affected by Costello syndrome with fasting hypoglycemia and short stature , associated low circulating levels of acid labile subunit ( ALS ) , relatively low levels of IGF 1 and IGFBP 3 , and normal IGF 2 , mostly circulating in a binary complex with IGFBP 2 and 6 instead of in a 150 kDa ternary complex . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Especially human IGFBP 3 but also bovine IGFBP 2 added to cell cultures inhibited the rate of proliferation of C2C12 myoblasts stimulated by human IGF 1 . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
To further examine the utility of IGF F 1 1 as an IGF 1 antagonist we tested its ability to inhibit IGFBP 2 and IGFBP 3 binding to IGF 1 , ( 125 ) 1 IGF 1 binding to IGF 1Rs and to block IGF 1 induced Akt activation , cell cycle changes and [ ( 3 ) H ] thymidine incorporation in MCF 7 cells . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Therefore , we investigated the dynamic changes in circulating total , free , and bioactive IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 , and IGFBP 1 bound IGF 1 ( binary complex ) during an oral glucose tolerance test ( OGTT ) in patients with liver cirrhosis . ^^^ Blood samples were drawn at 0 , 30 , 60 , 90 , 120 , 150 , and 180 min for determination of serum total and free IGF 1 , IGFBP 1 , IGFBP 2 , and binary complex , while bioactive IGF 1 was measured at 0 , 30 , 60 , 120 , and 180 min . ^^^ RESULTS : In comparison with healthy subjects , baseline levels of total ( 47 % ) , free ( 36 % ) , and bioactive IGF 1 ( 51 % ) were lower , while IGFBP 1 ( 268 % ) was higher ( P < 0 . 05 ) , IGFBP 2 ( 172 % ) tended to be higher ( P > 0 . 05 ) , and the binary complex unchanged ( approximately 100 % ) in cirrhotic patients . ^^^ Serum total and free IGF 1 , and IGFBP 2 remained unchanged in both study groups during the OGTT . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Northern blot analysis suggested IL 1beta regulation of IGF 1 and , to some extent , IGF IR was mediated by transcription ; however , the cytokine did not affect IGFBP 2 expression . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
METHODS : Fasting IGFBP 2 , IGFBP 1 , IGFBP 3 , IGF 1 , IGF 2 , insulin , C peptide , glucose , lipids , NEFAs , and HbA1c were measured in a cohort of 163 patients with type 2 diabetes . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
Using the Bonferroni criterion , we find that dwarf mice show increases in liver mRNA for two mannose binding lectins , two DNA binding proteins , serum amyloid P component , corticosteroid binding globulin , and insulin like growth factor binding protein 2 , as well as decreases in a two phosphodiesterases , a pheromone binding urinary protein , insulin like growth factor 1 ( IGF 1 ) , a calcium binding protein calgranulin B , a deubiquitinating enzyme , a hydroxysteroid dehydrogenase , a DNA methyltransferase , a glycine transporter , and a placental lactogen . ^^^
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P18065 and P05019	Pubmed	SVM Score :0.0
NA