Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.74345849
IGFBP 6 binds IGF 2 with high affinity and prevents IGF 2 mediated effects . 0.74345849^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.74345849
IGFBP 6 binds IGF 2 with high affinity and prevents IGF 2 mediated effects . 0.74345849^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.77997093
IGFBP 6 binds IGF 2 with high affinity and prevents IGF 2 mediated effects , so that a possible mode of regulating the IGF 2 available to bone cells would be by changing the levels of IGFBP 6 . 0.77997093^^^ Since IGFBP 6 binds IGF 2 and prevents its effects on bone cells , decreased synthesis of IGFBP 6 induced by TGF beta 1 could be a local feedback mechanism to increase the amount of IGF 2 available in the bone microenvironment . . 0.72715719^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.62578394
The increase in IGFBP 6 concentration coupled with its high affinity for IGF 2 may also contribute to decreased cellular proliferation by an indirect mechanism involving sequestration of endogenously produced IGF II . . 0.62578394^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Enzymatic deglycosylation did not alter the high affinity of IGF binding protein 6 for IGF 2 ( Ka 4 . 4 + / 2 . 2 10 10 ( 11 ) M 1 ) or its preference for IGF 2 over IGF 1 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In contrast , 48 h exposure to retinoic acid increased IGF 2 polypeptide levels , possible due to increased levels of IGF binding protein 6 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Sheep fibroblasts produced seven IGFBPs : a 36 . 5 41 kDa protein induced in vitro by IGF 1 , likely representing oIGFBP 3 ; a 28 . 5 kDa protein that reacted with antisera to human IGFBP 2 , likely representing oIGFBP 2 ; 25 and 27 kDa proteins induced in fetal fibroblasts by IGF 1 ; a 22 kDa protein that was inhibited by IGF 1 , likely representing oIGFBP 4 ; and 21 and 23 kDa proteins labelled only by IGF 2 , suggesting their similarities to IGFBP 6 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The binding proteins IGFBP 1 and IGFBP 3 did not cross react , but both IGF 1 and IGF 2 markedly inhibited IGFBP 6 tracer binding to antiserum . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Mouse IGFBP 6 mRNA expression was increased in IGF 2 overexpressing or IGF 2 treated control cells . ^^^ Upon acidification of the medium samples , only the degradation of IGFBP 6 was prevented in IGF 2 overexpressing cell conditioned medium . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
These results indicate that the expression of IGF 2 , IGFBP 2 , and IGFBP 6 is regulated in a differentiation dependent manner in Caco 2 cells . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In situ hybridization analysis revealed that IGF 2 , IGFBP 5 , and IGFBP 6 mRNAs were localized in the stromal component of the tumor , whereas IGFBP 2 mRNA was expressed by epithelial cells . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 6 ( IGFBP 6 ) is an O linked glycoprotein that binds insulin like growth factor 2 ( IGF 2 ) with marked preferential affinity over IGF 1 . ^^^ These results are consistent with the preferential affinity of IGFBP 6 for IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 3 and IGFBP 6 were used to study whether both IGF 1 and IGF 2 play a role in auto / paracrine stimulation of rat osteoblast growth . ^^^ Consistent with their IGF binding affinities , IGFBP 3 blocked both IGF 1 and IGF 2 stimulated DNA synthesis , whereas IGFBP 6 preferentially blocked IGF 2 stimulated DNA synthesis . ^^^ Because IGFBP 6 preferentially binds IGF 2 and is much less potent than IGFBP 3 in decreasing basal DNA synthesis in calvaria cells , IGF 1 but not IGF 2 appears to be an important auto / paracrine stimulator of DNA synthesis . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
This study indicates ( a ) that IGF 2 is potentially capable of autocrine regulation on the basolateral side of HT 29 D4 GAL cell , and ( b ) that IGFBP 6 has a unique pattern of secretory polarity . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Enzymatic deglycosylation does not alter the high affinity of IGFBP 6 for IGF 2 ( Ka 4 . 4 + / 2 . 2 10 10 ( 11 ) M 1 ) or its preference for IGF 2 over IGF 1 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 1 and IGFBP 6 are most sensitive to changes in IGF 2 structure , although IGFBP 1 binds IGF 1 and IGF 2 with equal affinity , whereas IGFBP 6 has a marked preferential binding affinity for IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Competitive binding studies of rat and human IGFBP 6 with [ 125I ] IGF 2 and unlabeled IGF 2 or IGF 1 demonstrated that both IGFBPs bound IGF 2 with similar affinities ( Ka , 1 . 5 1 . 8 10 10 ( 11 ) M 1 ) and bound IGF 1 with approximately 25 to 35 fold lower affinity than IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
To gain insight into the role of the insulin like growth factors ( IGFs ) in regulating lung development , we have used in situ hybridization histochemistry ( ISHH ) to examine the ontogeny and sites of expression of IGF 1 and IGF 2 , IGF binding proteins ( IGFBP 1 to IGFBP 6 ) , and IGF cell surface receptors in fetal rat lung from 15 to 21 days of gestation . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In this study , SK N SH neuroblastoma cells were shown to produce IGF 2 , IGFBP 2 , IGFBP 4 and small quantities of IGFBP 6 . ^^^ Insulin like growth factor binding protein 6 has a strong affinity for IGF 2 , 5 10 times that of IGFBP 2 and at least 10 times that of the type 1 IGF receptor , and the arrested proliferation may result , at least in part , from sequestration by IGFBP 6 of the IGF 2 secreted . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
PEG did not precipitate the complex of 125I IGF II / IGFBP 6 while it precipitated the complexes between 125I IGF 2 and other IGFBPs . ^^^ Indeed , IGFBP 6 decreased the amount of 125I IGF 2 tracer in the PEG precipitate in a dose dependent manner . ^^^ Also , the binding of IGFBP 6 with 125I IGF 2 was poor in Western ligand blots compared with other IGFBPs . ^^^ In studies on IGFBP 6 actions , IGFBP 6 completely inhibited IGF 2 induced [ 3H ] thymidine incorporation in MC3T3 E 1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF 1 induced [ 3H ] thymidine incorporation . ^^^ This differential effect is associated with the fact that IGFBP 6 has greater affinity for IGF 2 than IGF 1 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In early differentiated cells , i . e . , up to day 7 post confluence , IGF 2 , IGFBP 2 , IGFBP 4 ( > 96 % ) and IGFBP 6 ( approximately 85 % ) are recovered in the basolateral medium . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The three human cells also produced a 30K IGFBP , shown to be IGFBP 6 , based on increased affinity for IGF 2 vs . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
At high cell density and with high concentrations of IGF 1 , des ( 1 3 ) IGF 1 ( 40 ng / ml ) or IGF 2 ( 80 ng / ml ) , the mitogenic activities of the IGFs diminished concomitantly with the appearance in the culture medium of an additional IGFBP identified as IGFBP 6 , whose production depended on activation of the type 1 IGF receptor . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Fasting levels of IGF 1 , free IGF 1 , IGF 2 , IGFBP 3 , and insulin increased significantly within 3 months of GH treatment , whereas IGFBP 1 , IGFBP 2 , and IGFBP 6 showed no change . ^^^ IGFBP 3 and IGF 2 both showed late ( > 20 h ) responses to a dose of GH , whereas IGFBP 2 and IGFBP 6 showed minimal changes . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In summary , a specific antiserum and RIA were used to demonstrate elevated levels of intact IGF 2 binding IGFBP 6 in serum of CRF children . ^^^ We postulate that the excess IGFBP 6 may modulate the action of IGF 2 on target tissues . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
HT 29 D4 cells secreted IGF 2 totally complexed to IGFBP 2 , IGFBP 4 and IGFBP 6 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Incubation of PC 12 cells with IGF 2 ( 100 ng / ml ) for 72 h increased IGFBP 6 protein levels in media to 217 + / 19 % of control ( P < 0 . 0001 ) . ^^^ The effects of IGF 2 and dexamethasone on IGFBP 6 levels were independent . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Analyses of the cell cycle ( flux cytofluorometry ) , mitogenic activity ( radiolabeled thymidine incorporation ) , and the number of viable cells ( 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide test ) showed that the mitogenic effects of serum , IGF 1 , IGF 2 , and des ( 1 3 ) IGF 1 , a truncated IGF 1 analogue with no affinity for IGFBP 6 , were depressed in both transfected cell lines . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The actions of insulin like growth factors ( IGFs ) are modulated by a family of high affinity binding proteins ( IGFBPs ) , including IGFBP 6 , which preferentially binds IGF 2 and is O glycosylated . ^^^ Glycosylated and nonglycosylated recombinant human IGFBP 6 , expressed in Chinese hamster ovary cells and Escherichia coli , respectively , were purified using IGF 2 affinity chromatography and reverse phase medium pressure chromatography . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
This study describes the expression of the mouse IGFBP 6 which is unique among IGFBPs in its preferential binding of IGF 2 , in insect cells using the baculovirus system . ^^^ The purified , O glycosylated IGFBP 6 was functional as shown by IGF binding and by inhibition of IGF 2 stimulated DNA synthesis in human fibroblasts . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Whereas 6 6 5 , like IGFBP 6 , had a marked preference for binary complex formation with IGF 2 rather than IGF 1 , it formed ternary complexes more efficiently with IGF 1 , like IGF BP 5 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Factors contributing to the defect are : ( 1 ) low ALS levels , secondary to suppressed growth hormone ( GH ) ; ( 2 ) defective IGFBP 3 binding to ALS ; ( 3 ) reduced ability of pro IGF 2 forms to complex normally , and ( 4 ) very high levels of other IGFBPs , including IGFBP 2 and IGFBP 6 , which might limit the formation of complexes with IGFBP 3 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
HaCaT human keratinocytes express IGF 2 , IGFBP 6 , and an acid activated protease with activity against IGFBP 6 . ^^^ In the present study , IGFBP 6 was detected in HaCaT conditioned medium by use of immunoblotting and Western ligand blotting with 125I labeled IGF 2 . ^^^ Although proteolysis did not substantially reduce the size of immunoreactive IGFBP 6 , it greatly reduced the ability of IGFBP 6 to bind 125I IGF 2 as determined by Western ligand blotting and solution assay . ^^^ These observations suggest the possibility of an autocrine IGF 2 loop that is regulated by the relative expression of IGF 2 , IGFBP 3 , and IGFBP 6 , and IGFBP proteases in these keratinocytes , although demonstration of this loop requires further study . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 6 ( IGFBP 6 ) differs from IGFBPs 1 5 in that it binds IGF 2 with marked preferential affinity over IGF 1 . ^^^ IGFBP 6 is a relatively specific inhibitor of IGF 2 actions ; it has not been shown to potentiate IGF actions . ^^^ Although many questions remain regarding the biological role of IGFBP 6 , its major function appears to be the regulation of IGF 2 actions . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGF2R / IGF 2 deficient mice have elevated levels of circulating IGF binding protein ( IGFBP ) 3 and show a strong IGFBP 6 immunoreactivity in all pancreatic islet cells and in secretory granules of different size in acinar cells and interlobular connective tissue of exocrine pancreas . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
As expected , IGFBP 6 was visible only when IGF 2 was used as radioligand . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 differs from other IGFBPs in having the highest affinity for IGF 2 and in binding IGF 1 with 20 100 fold lower affinity . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
After stable transfection with N myc cDNA , Northern blotting revealed a marked increased in IGF IR , IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 4 mRNA levels , whereas IGFBP 6 mRNA levels were clearly diminished . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
A distinctive property of IGFBP 6 is its preferential affinity for IGF 2 relative to IGF 1 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Tumour cyst fluid contained excessive amounts of both big IGF 2 and IGFBP 6 . ^^^ Northern blot analysis of total mRNA isolated from the tumour demonstrated high expression of the IGF 2 gene and abundant 1 . 1 kb IGFBP 6 transcript , while the genes encoding IGFBP 3 , 4 and 5 were only weakly expressed and mRNA of IGFBP 1 , 2 and IGF 1 could not be detected . mRNAs for the IGF type 2 receptor could be easily demonstrated , whereas those for the insulin and IGF type 1 receptor were hardly detectable . ^^^ The present study suggests an important role of the simultaneous production of IGF 2 and IGFBP 6 in the pathophysiology of tumour induced hypoglycaemia . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Production of IGF 1 , IGF 2 , and IGFBP 6 by the cells or significant degradation of rhIGFBP 6 could not be detected within 24 h of incubation . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Because the anatomical distribution of IGFBPs is likely to dictate IGF bioavailability , we determined the cellular distribution and expression of IGF 1 , IGF 2 , and IGFBP 1 to IGFBP 6 in epiphyseal growth plates of the fetal sheep , using immunocytochemistry and in situ hybridization . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In the spleen , IGFBP 6 expression was not altered by IGF 2 overexpression , whereas IGFBP 3 expression was strongly increased . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
A positive correlation was observed between IGF 2 and IGFBP 6 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 , an O linked glycoprotein , preferentially binds IGF 2 and inhibits its actions . ^^^ CONCLUSIONS : O glycosylation delays the clearance of IGFBP 6 from the circulation and may therefore contribute to its role as a circulating inhibitor of IGF 2 actions . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
When bound to glycosaminoglycans , IGFBP 6 had approximately 10 fold reduced binding affinity for IGF 2 . ^^^ Proteolysis decreased the binding affinity of IGFBP 6 for IGF 2 , even with a relatively small reduction in apparent molecular mass as observed with chymotrypsin . ^^^ In contrast , trypsin cleaved IGFBP 6 in the mid region of the molecule , resulting in a 16 kDa C terminal peptide which did not bind IGF 2 . ^^^ These results indicate that O glycosylation inhibits binding of IGFBP 6 to glycosaminoglycans and cell membranes and inhibits its proteolysis , thereby maintaining IGFBP 6 in a high affinity , soluble form and so contributing to its inhibition of IGF 2 actions . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The effects of cAMP agonists and IGF 2 , which increases IGFBP 6 protein but not mRNA levels , were not inhibited by rapamycin , suggesting that p 70 S6 kinase is not involved . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The expression of IGF 2 and IGFBP 1 to IGFBP 6 mRNAs was determined by in situ hybridization in the Fallopian tubes of extrauterine pregnancies and concurrent decidua ( n = 6 ) , and in the placentae and Fallopian tubes of intrauterine pregnancies ( n = 6 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Northern blot analyses if xenografts revealed weaker expression of IGF 2 , type 2 IGF receptor and IGFBP 2 mRNAs in IGFBP 6 expressing cthan in control xenografts . ^^^ IGFBP 6 may therefore reduce the expression of IGF 2 ( which induces tumour development ) at a transcriptional level . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The insulin like growth factor ( IGF ) system includes IGF 1 and IGF 2 , the type 1 and type 2 IGF receptors , and specific IGF binding proteins ( IGFBP 1 to IGFBP 6 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 is unique among the IGFBPs in its preferential binding of IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The proteolytic activity against IGFBP 6 differed at least from IGFBP 5 protease activity in its sensitivity both to IGF 2 and to the hydroxamic acid based disintegrin metalloprotease inhibitor , as well as serine protease inhibitors . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Rhabdomyosarcoma cell lines overexpress insulin like growth factor 2 ( IGF 2 ) , an autocrine growth factor that is inhibited by insulin like growth factor binding protein 6 ( IGFBP 6 ) . ^^^ IGF 2 partially reversed the IGFBP 6 induced decrease in growth and increase in apoptosis . ^^^ IGFBP 6 therefore inhibits proliferation and promotes apoptosis of rhabdomyosarcoma in vitro and dramatically inhibits xenograft growth in vivo , at least in part by inhibiting IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The concentrations of IGFBP 2 and IGFBP 4 decreased by 48 + / 6 and 70 + / 13 % , respectively , whereas that of IGFBP 6 increased by 698 + / 20 % with 1 microM tRA . tRA decreased mRNA levels of IGFBP 2 and IGFBP 4 by 20 + / 3 and 50 + / 8 % , respectively , whereas tRA increased IGFBP 6 mRNA by 660 + / 20 % . tRA did not alter levels of IGF 2 mRNA or peptide . ^^^ Increased expression of IGFBP 6 , which has a high binding affinity for IGF 2 , following tRA treatment suggests that the decreased proliferation caused by tRA may result , at least in part , from IGFBP 6 mediated disruption of the IGF 2 autocrine loop in these colon cancer cells . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 SDS was low at baseline ( 2 . 5 ) and rose to 1 . 9 and 1 . 7 IGF 2 and IGFBP 1 showed an irregular pattern . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGF 2 was more strongly expressed in control tumours , and IGFBP 6 expressing tumours contained less IGFBP 2 than controls . ^^^ In cell culture , IGFBP 6 expressing cells expressed similar amounts of IGFBP 2 , IGF 2 and N myc mRNAs as control cells ; but media conditioned by IGFBP 6 expressing cells contained less intact IGFBP 2 protein , with no increase in its proteolytic fragment . ^^^ With its especially strong affinity for IGF 2 and its resistance to proteolysis , IGFBP 6 would act by sequestering IGF 2 , hence inhibiting its mitogenic and anti apoptotic effects . ^^^ In excess , IGFBP 6 would displace IGF 2 from IGFBP 2 whose potentiation of IGF 2 action would cease and whose susceptibility to degradation would be increased . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 6 ( IGFBP 6 ) is a high affinity IGFBP with substantially greater affinity for insulin like growth factor 2 ( IGF 2 ) than IGF 1 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
With a view to blocking IGF 2 action , we produced recombinant human IGFBP 6 capable of binding IGFs with affinities between 1 . 23 and 6 . 36 10 10 ( 9 ) M ( 1 ) . ^^^ Ex vivo mitogenic activities were tested on two human neuroblastoma cell lines , in which 100 ng / ml IGFBP 6 completely abolished the effects of both endogenous and exogenous IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
In conclusion , IGFBP 6 mRNA is differentially regulated during the estrous cycle and pregnancy and may be functionally important in modulating IGF activity in the uterus and placenta by virtue of its strong affinity and ability to regulate IGF 2 mediated actions . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Although they appeared as cellular clusters with a uniform epithelial phenotype , immunohistochemical analyses of components of the IGF system revealed an unique pattern : weak immunoreactivity could be seen for IGF 2 while among all IGF binding proteins ( IGFBPs ) only IGFBP 6 and weakly IGFBP 4 were detectable in epithelial cells of Malassez . ^^^ Since IGFBP 6 has a very high affinity for IGF 2 and can inhibit its functions , we discuss that , in the normal periodontal ligament , autocrine IGFBP 6 may function as an antiproliferative molecule suppressing mitogenic effects of IGFs on Malassez cells . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
With in situ hybridization , insulin like growth factor 2 ( IGF 2 ) mRNA and insulin like growth factor binding protein 2 ( IGFBP 2 ) , 3 and 6 were all detected in the placentome capsule ; IGF 2 mRNA was also found in the mesoderm of the fetal villi and IGFBP 3 and IGFBP 6 were present in the caruncular stroma of the maternal villi . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
CONCLUSIONS : : Our findings are consistent with the hypothesis that IGFBP 6 inhibits cell growth by binding to endogenously produced IGF 2 , thereby preventing IGF 2 from interacting with the IGF 1 receptor to stimulate cellular proliferation by an autocrine mechanism . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Brain invasiveness in degrees from respect of the arachnoid membrane progressing to frank brain invasion correlated with increases in IGF 2 and IGFBP 6 expression . ^^^ High levels of IGFBP 6 may not inhibit IGF 2 actions , which is known to be growth promoting in tumors . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^ When VA deficient rats received a single injection of retinoic acid ( 2 mg / rat ) , tissue IGF 1 and IGF IR gene expression did not change after 4 or 8 h , while the expression of IGF 2 , IGFBP 4 , and IGFBP 6 mRNAs in some tissues increased rapidly . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 6 were examined by specific assays . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGF 2 induced migration was abolished by insulin like growth factor binding protein 6 ( IGFBP 6 ) ( P < 0 . 01 ) , a relatively specific inhibitor of IGF 2 , and by plasminogen activator inhibitor type 1 ( PAI 1 ) ( P < 0 . 05 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The IGF 2 and IGFBP 6 mRNA levels in rat calvaria bone tissue and MC 3T3 E 1 cells were detected by reverse transcription polymerase chain reaction . ^^^ The expression of IGF 2 mRNA gene was enhanced by estrogen in rat calvaria bone tissue and MC 3T3 E 1 cells , whereas IGFBP 6 mRNA was decreased . ^^^ TCDD , however , inhibited the effect of estrogen on regulation of IGF 2 gene and IGFBP 6 gene as well as MC 3T3 E 1 cell proliferation . ^^^ TCDD has antiestrogenic effect and hence exerts negative influence on the osteoblast cells through target IGF 2 and IGFBP 6 of IGFs family . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Treatment of Rh 30 rhabdomyosarcoma cells with insulin like growth factor binding protein 6 ( IGFBP 6 ; 1000 ng / ml ) , which binds insulin like growth factor 2 with high affinity , inhibited growth in vitro ( p < 0 . 001 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 had virtually no inhibitory effects on IGF 1 , but was moderately effective against IGF 2 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Within the family of six binding proteins , IGFBP 6 is unique in having a 20 100 fold higher affinity for IGF 2 over IGF 1 and appears to act primarily as an inhibitor of IGF 2 actions . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 is unique in its 20 100 fold IGF 2 binding specificity over IGF 1 . ^^^ We confirmed that differential dissociation kinetics are responsible for the IGF 2 binding preference of IGFBP 6 . ^^^ The N domain has rapid association kinetics , similar to full length IGFBP 6 , but both IGF 1 and 2 dissociate rapidly from this domain , thereby reducing its binding affinity for IGF 2 approximately 50 fold . ^^^ This suggests that the C domain confers the IGF 2 binding preference of IGFBP 6 ; indeed , IGF 1 bound inconsistently with very low affinity to the C domain . ^^^ The results of the binding studies are supported by the effects of the IGFBP 6 domains on IGF induced colon cancer cell proliferation ; the N domain inhibited IGF 2 induced proliferation with approximately 20 fold lower potency than IGFBP 6 and it was equipotent in inhibiting IGF 1 and IGF 2 induced proliferation . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
A comparative analysis including eight previous gene expression studies revealed eight prominent genes ( ADH 1 , ATF 3 , CRABP 2 , CYR 61 , DPT , GRIA 2 , IGF 2 , MEST ) identified by at least five different studies , eleven genes ( ALDH 1 , CD 24 , CTGF , DCX , DUSP 1 , FOS , GAGEC 1 , IGFBP 6 , PTGDS , PTGER 3 , TYMS ) reported by four studies , twelve genes ( ABCA , ANXA 1 , APM 2 , CCL 21 , CDKN1A , CRMP 1 , EMP 1 , ESR 1 , FY , MAP3K5 , TGFBR 2 , TIMP 3 ) identified by three studies , and 40 genes reported by two different studies . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Igfbp 6 is a secreted factor that specifically binds insulin like growth factor 2 ( IGF 2 ) , prevents its binding to the IGF 1 receptor , and is thought to regulate the activity of IGF 2 in growth and differentiation . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGFBP 6 inhibits growth of a number of IGF 2 dependent cancers , including rhabdomyosarcoma , neuroblastoma and colon cancer . ^^^ Although the major action of IGFBP 6 appears to be inhibition of IGF 2 actions , a number of studies suggest that it may also have IGF independent actions . ^^^ Both the N terminal and C terminal domains of IGFBP 6 contribute to high affinity IGF binding , and the C terminal domain appears to confer its IGF 2 specificity . ^^^ The three dimensional structure of the C domain of IGFBP 6 contains a thyroglobulin type 1 fold , and the IGF 2 binding site is located in the proximal half of this domain adjacent to the glycosaminoglycan binding site . ^^^ This is particularly significant since IGFBP 6 provides an attractive basis for therapy of IGF 2 dependent tumors . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
IGF 2 , IR B , IGFBP 1 , IGFBP 4 , and IGFBP 6 mRNA were downregulated in seminoma . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The insulin like growth factor 2 ( IGF 2 ) and IGF conjugated protein 6 ( IGFBP 6 ) mRNA level in rat calvaria bone tissue and MC 3T3 E 1 cells were detected by northern blotting analysis and reverse transcription polymerase chain reaction . ^^^ The expression of IGF 2 mRNA and cell proliferation were enhanced by E 2 ( 1 10 10 ( 6 ) mol / L ) in rat calvaria bone tissue and MC 3T3 E 1 cells , whereas the IGFBP 6 mRNA was increased . ^^^ IGF 2 and IGFBP 6 are important regulative factors for skeleton development and osteoblast proliferation in rat . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
The 31 , 000 Mr protein was identified as IGF BP 2 by specific immunoreactivity with two polyclonal antibodies , and the 24 , 000 21 , 500 Mr doublet was identified as IGF BP 6 by reactivity with a specific polyclonal antibody and by marked preferential affinity for IGF 2 over IGF 1 by ligand blotting . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Microarray analysis of implantation and explantation samples of recovery patients further revealed elevated IGF 2 and IGF binding proteins IGFBP 4 and IGFBP 6 . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Among genes that were most significantly upregulated in carcinomas were 2 ubiquitin related genes ( USP 4 and UFD1L ) and several insulinlike growth factor related genes ( IGF 2 , IGF2R , IGFBP 3 and IGFBP 6 ) . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Plasma concentrations of GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 did not demonstrate any significant differences between treatment groups and controls . ^^^ Changes in the systemic osteotropic growth factors GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 do not seem to be of importance during distraction osteogenesis . . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
Differential dissociation kinetics explain the binding preference of insulin like growth factor binding protein 6 for insulin like growth factor 2 over insulin like growth factor 1 . ^^^ Insulin like growth factor binding protein 6 binds insulin like growth factor 2 with a marked preferential affinity over insulin like growth factor 1 . ^^^ Binding of insulin like growth factor 1 and insulin like growth factor 2 to immobilized insulin like growth factor binding protein 6 fitted a two site binding kinetic model . ^^^
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA
Interacting proteins: P24592 and P01344	Pubmed	SVM Score :0.0
NA