| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.5281661 |
| There was also differential ethanol sensitivity of IRS 2 and Shc phosphorylation , and the association of Shc with IRS 2 , among the different cell types . 0.5281661^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 2 and Shc play different roles in insulin like growth factor 1 signaling . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Insulin is an important regulator of growth and initiates its action by binding to its receptor , which undergoes tyrosyl autophosphorylation and further enhances its tyrosine kinase activity towards other intermediate molecules , including insulin receptor substrate 1 , insulin receptor substrate 2 , and Shc . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| In addition , insulin receptor substrate 2 ( IRS 2 ) protein levels were reduced in IUGR placentas , with no changes in IRS 1 or Shc protein content , and this was associated with a parallel decrease in IRS 2 associated phosphatidyl inositol 3 kinase . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Stimulation of the insulin receptor ( IR ) results in tyrosine phosphorylation of the intermediate molecules insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , and Shc , which then couple the IR to downstream signaling pathways by serving as binding sites for signaling molecules with SH 2 domains . ^^^ It has been proposed that direct binding of IRS 1 , IRS 2 , and Shc to an NPX Tyr ( P ) motif in the juxtamembrane region of the IR is required for tyrosine phosphorylation of these molecules by the IR . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| At present we feel the use of the name PTB domain should be reserved for Shc and IRS 1 / IRS 2 , where phosphotyrosine binding has been demonstrated . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Insulin ' s effects are initiated by insulin binding to the insulin receptor , resulting in tyrosine phosphorylation of insulin receptor and intracellular substrates , such as insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , or Shc . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Embryonic fibroblasts and 3T3 cell lines derived from IRS 1 deficient embryos exhibit no IGF 1 stimulated IRS 1 phosphorylation or IRS 1 associated phosphatidylinositol 3 kinase ( PI 3 kinase ) activity but exhibit normal phosphorylation of IRS 2 and Shc and normal IRS 2 associated PI 3 kinase activity . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| The ability of insulin to stimulate protein synthesis and cellular growth is mediated through the insulin receptor ( IR ) , which phosphorylates Tyr residues in the insulin receptor substrate signaling proteins ( IRS 1 and IRS 2 ) , Gab 1 , and Shc . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Activated insulin receptor ( IR ) interacts with its substrates , IRS 1 , IRS 2 , and Shc via the NPXY motif centered at Y 960 . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulation results in a considerable spectrum of cellular responses , only part of which have been firmly correlated with the activation of established insulin receptor ( IR ) targets such as IRS 1 , IRS 2 , and Shc . ^^^ Many responses may be transduced by alternative direct IR targets , some of which may still be unknown , may act in parallel to but independently of IRS 1 , IRS 2 , and Shc , and may be members of the growing family of SH 2 domain containing signaling adaptors . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Our results suggest that both IRS 1 and IRS 2 are required for phosphatidylinositol 3 kinase activation that leads to adipogenic and thermogenic differentiation of fetal brown adipose tissue ; meanwhile , IRS 1 and SHC , but not IRS 2 , associate with Grb 2 leading to the ras mitogen activated protein kinase signaling pathway required for fetal brown adipocyte proliferation . . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , survival signaling was dependent upon tyrosine 950 , the binding site for IRS 1 , IRS 2 , and Shc proteins . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| In cultured cells , GH stimulates the tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , and Shc . ^^^ GH increased the tyrosine phosphorylation of IRS 1 , IRS 2 , JAK 2 , and Shc proteins in the liver , heart , kidney , muscle , and adipose tissue of rats . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Contraction also resulted in an apparent increase in the association of Raf 1 with p21Ras , although stimulation of MAP kinase signaling occurred independent of Shc , IRS 1 , and IRS 2 tyrosine phosphorylation or the formation of Shc / Grb2 or IRS1 / Grb2 complexes . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Despite this , a variety of PTB or SH 2 domain containing signaling proteins , including IRS 2 , mSos 1 , Shc , p 85 subunit of phosphatidylinositol 3 kinase , SHP 2 , Raf 1 , and JAK 2 , were not associated with the INR beta Myc . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| In response to insulin , tyrosine kinase activity of the insulin receptor is stimulated , leading to autophosphorylation and tyrosine phosphorylation of proteins including insulin receptor subunit ( IRS ) 1 , IRS 2 , and Shc . ^^^ After 1 min of insulin stimulation , the insulin receptor was tyrosine phosphorylated 8 fold more and Shc was phosphorylated 50 % less in 32D cells containing both IRS 1 and insulin receptors ( 32D / IR+IRS 1 ) than in 32D cells containing only insulin receptors ( 32D / IR ) , insulin receptors and IRS 2 ( 32D / IR+IRS 2 ) , or insulin receptors and a form of IRS 1 that can not be phosphorylated on tyrosine residues ( 32D / IR+IRS 1F18 ) . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Glucose ( > 3 mM ) independently increased tyrosine phosphorylation mediated recruitment of growth factor bound protein 2 ( Grb 2 ) / murine sons of sevenless 1 protein ( mSOS ) and PI3 ' K to insulin receptor substrate ( IRS ) 1 and IRS 2 , as well as SH 2 containing protein ( Shc ) association with Grb2 / mSOS and downstream activation of mitogen activated protein kinase and 70 kDa S 6 kinase . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| IL 4 induced the association of tyrosine phosphorylated Shc with the IL 4Ralpha , whereas no detectable tyrosine phosphorylation of IRS 1 or IRS 2 was induced . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| These effects are mediated by changes in tyrosine phosphorylation of IGF 1 receptor substrates , including IRS 2 and Shc . . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| We studied the impact of 16 serine residues in HIR by mutation to alanine and co overexpression in human embryonic kidney ( HEK ) 293 cells together with the docking proteins insulin receptor substrate ( IRS ) 1 , IRS 2 , or ( SHC ) Src homologous and collagen like . ^^^ Coexpression of HIR with IRS 1 , IRS 2 , and SHC strongly enhanced tyrosine phosphorylation of these proteins . ^^^ A similar increase in tyrosine phosphorylation was observed in cells overexpressing IRS 1 , IRS 2 , or SHC together with all HIR mutants except HIR delta JM and a mutant carrying exchanges of serines 1177 , 1178 , and 1182 to alanine ( HIR1177 / 78 / 82 ) , although this mutant showed normal autophosphorylation . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Ret oncogene signal transduction via a IRS 2 / PI 3 kinase / PKB and a SHC / Grb 2 dependent pathway : possible implication for transforming activity in NIH3T3 cells . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| It stimulated tyrosine phosphorylation of the type 1 IGF receptor ( IGF IR ) beta subunit , IRS 1 , IRS 2 , and Shc , and these changes were associated with activation of Erk and Akt . ^^^ PD 98059 inhibited activation of Erk and LY 294002 repressed activation of Akt in response to IGF 1 , but did not affect tyrosine phosphorylation of the IGF IR , IRS 1 , IRS 2 , or Shc . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| In conclusion , in the diabetic heart , 1 ) IRS 1 , IRS 2 , and p 52 ( Shc ) are differently altered , 2 ) the levels of Akt phosphorylation on Ser 473 and Thr 308 , respectively , are not coordinately regulated , and 3 ) the increased activity of proximal signaling proteins ( i . e . , IRS 2 and PI 3 kinase ) is not propagated distally to GSK 3 . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Down regulation of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc gene expression by insulin in skeletal muscle is not associated with insulin resistance or type 2 diabetes . ^^^ To examine whether altered gene expression of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc is an inherited trait and is associated with muscle insulin resistance or type 2 diabetes , we measured mRNA levels of these genes by a relative quantitative RT PCR method in muscle biopsies taken before and after an insulin clamp from 12 monozygotic twin pairs discordant for type 2 diabetes and 12 control subjects . ^^^ Basal mRNA levels of IRS 1 , IRS 2 , and Shc were similar in the diabetic and nondiabetic twins as well as in the control subjects . ^^^ Insulin decreased mRNA expression of IRS 1 by 72 % ( from 0 . 75 + / 0 . 06 to 0 . 21 + / 0 . 04 relative units ; P < 0 . 001 ) , IRS 2 by 71 % ( from 0 . 55 + / 0 . 10 to 0 . 16 + / 0 . 08 relative units ; P < 0 . 03 ) , and Shc by 25 % ( from 0 . 95 + / 0 . 04 to 0 . 71 + / 0 . 04 relative units ; P < 0 . 01 ) vs . baseline as demonstrated in the control subjects . ^^^ The postclamp Shc mRNA level was slightly higher in the diabetic twins ( P = 0 . 05 ) but similar in the nondiabetic twins , as compared with the control subjects , whereas postclamp IRS 1 and IRS 2 mRNA levels were similar between the study groups . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| After receptor binding , growth hormone ( GH ) induces GH receptors ( GHR ) dimerization and JAK 2 is activated after its association with a dimerized GHR , stimulating the tyrosyl phosphorylation of insulin receptor substrate 1 ( IRS 1 ) , IRS 2 and Shc proteins . ^^^ Additionally , GH induced IRS 1 , IRS 2 and SHC tyrosyl phosphorylation was inhibited approximately 50 % at equimolar concentrations of the antagonist of GH and almost abolished with a GH : G120K PEG ratio of 1 : 100 . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| EGF increased the tyrosine phosphorylation of several proteins ( the EGF receptor , insulin receptor substrate ( IRS ) 1 , IRS 2 , and Grb 2 associated binder 1 ) , whereas Shc and Gab 2 were only weakly and inconsistently phosphorylated . p 85 , the regulatory subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) , was also found to associate with all of these docking molecules , showing that EGF activated PI 3 kinase pools that were additional to those of insulin . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Analysis of IL 9 receptor mutants showed that activation of the pathway was correlated with proliferation and with phosphorylation of the adaptor protein SHC , but not IRS 2 or GAB 2 . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| We investigated the effects of RA on the regulation of the IGF IR and its key signaling elements : IRS 1 , IRS 2 , and SHC . ^^^ IRS 1 regulation is selective , as RA did not influence IRS 2 or SHC levels . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Downstream of IRS 2 and Shc , IGF 1 treatment results in strong activation of Akt and MAPK in N cells and activation of both pathways is required for IGF 1 mediated differentiation . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| IRS 1 , IRS 2 and SHC 1 are the key mediators for the downstream pathway processes . ^^^ We screened IRS 1 , IRS 2 and SHC 1 for published coding region polymorphisms and choose five of them , IRS 1 Ala804Ala and Gly972Arg , IRS 2 Cys816Cys and Gly1057Asp and SHC 1 Met300Val , for further analysis . ^^^ A genotype combination analysis of the non synonymous polymorphisms in the IRS 1 , IRS 2 and SHC 1 genes did not show any effect on breast cancer risk . . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Higher basal levels of tyrosine phosphorylation were found in classic transducers of insulin cell signaling ( IRS 1 , IRS 2 and SHC ) . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| In spite of these differences , both FL Grb 10 and the BPS SH 2 fragment inhibited insulin stimulated phosphorylation of IRS 1 , IRS 2 , Akt / PKB , Shc , ERK1 / 2 , APS , and c Cbl to a similar extent . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| Overexpression studies in isolated islets have shown that IRS 2 , but not IRS 1 or Shc , is sufficient to induce proliferation of beta cells and to protect against d glucose induced apoptosis . ^^^ |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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