| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.73155576 |
| We conclude that IRS 2 , unlike IRS 1 , can interact with tyrosine phosphorylated receptors such as the IR and insulin like growth factor 1 receptor via multiple independent binding motifs . 0.73155576^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor 1 induced DNA synthesis in insulin secreting cell line RINm5F is associated with phosphorylation of the insulin like growth factor 1 receptor and the insulin receptor substrate 2 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| We report here that IGF 1 stimulated the tyrosine phosphorylation of the type 1 IGF receptor ( IGF IR ) and insulin receptor substrate 2 ( IRS 2 ) in a time and concentration dependent manner . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Taken together , these results demonstrate that up regulation and activation of IGF IR and the downstream signaling pathway involving insulin receptor substrate 2 , phosphatidylinositol 3 ' kinase , and p70S6K may play an important role in the development of a broad spectrum of plasma cell tumors . . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| We speculate that the IGF 1 activated IGF 1 receptor , in response to E 2 , directly or indirectly modifies insulin receptor substrate 2 , probably through phosphorylation , leading to ubiquitination and subsequent degradation of this docking protein by the proteasome . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| We found that insulin receptor substrate 2 ( IRS 2 ) levels were markedly induced by progesterone and the synthetic progestin R 5020 in MCF 7 and other progesterone receptor ( PR ) positive breast cancer cell lines , whereas IRS 1 and the IGF 1 receptor were not induced . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Deletion of the pleckstrin and phosphotyrosine binding domains of insulin receptor substrate 2 does not impair its ability to regulate cell proliferation in myeloid cells . 32D IGF 1 receptor ( IR ) cells are IL 3 dependent myeloid cells that can be induced to differentiate into granulocytes by IGF 1 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Both cell lines exhibit androgen responsive patterns of IGF 1 receptor ( IGF IR ) expression , activation , and signaling to insulin receptor substrate 2 and AKT . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| IGF 1 receptor and insulin receptor substrate 2 levels were increased in LTED versus the Wt MCF 7 cells , and ICI but not TAM reduced their expression in a dose dependent fashion . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Investigating the signaling pathway downstream of IGF 1R reveals that insulin receptor substrate 2 ( IRS 2 ) overexpression compensates for the lack of IGF 1R , whereas IRS 1 overexpression does not . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| IRS 2 was also Tyr phosphorylated in the normal uterus and bound more IGF 1R and p 85 in response to estradiol ; however , a marked decrease in levels of uterine IRS 2 occurred 12 24 h after treatment with estradiol . ^^^ Since IRS 2 was present in IGF 1R precipitates and a recombinant form of IGF 1 ( long R 3 IGF 1 ) stimulated formation of Tyr ( P ) IRS 2 , hormonal activation of this docking protein probably occurs through the IGF 1R . ^^^ In summary , our findings show that estrogen activation of uterine IGF 1R kinase results in enhanced binding of p 85 ( PI 3 kinase ) to IRS 1 and IRS 2 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Although the mitogenic function of the IGF IR may require the activation of insulin receptor substrate 1 ( IRS 1 ) or IRS 2 , an overexpressed IGF IR is able to protect 32D cells , which lack IRS 1 and IRS 2 , from apoptosis caused by Interleukin 3 ( IL 3 ) withdrawal . ^^^ Here , using mutational analysis , the authors identify domains of the IGF IR necessary to protect from apoptosis without downstream signaling from IRS 1 and IRS 2 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Genetic ablation experiments of insulin receptor substrate 1 ( IRS 1 ) and 2 ( IRS 2 ) , two important molecules in the IR and IGF IR signaling pathways , are also beginning to shed light onto the mechanisms accounting for the specificity of IR and IGF IR signaling . ^^^ IRS 1 deficient mice are growth retarded , while IRS 2 deficient mice develop diabetes , indicating that the two molecules play a more specific role than previously recognized in IGF IR and IR signaling . . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| We report here that the vitamin D analogue EB 1089 interferes with the IGF IR signaling pathway by attenuating IGF 1 induced tyrosine phosphorylation of IRS 1 , and to a lesser extent , IRS 2 . ^^^ It does not affect protein levels of IRS 1 , IRS 2 or IGF IR . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| These experiments indicate that TNF alpha promotes IGF 1 receptor resistance in neurons and inhibits the ability of the IGF 1 receptor to tyrosine phosphorylate the IRS 2 docking molecule and to subsequently activate the critical downstream enzyme phosphatidylinositol 3 ' kinase . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| To establish a role for the Igf 1 receptor in beta cells , we intercrossed mice heterozygous for null alleles of Igf1r and Irs 2 . ^^^ Irs 2 coordinates Igf 1 receptor mediated beta cell development and peripheral insulin signalling . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| In contrast , cell resistance to IGF 1 occurred at a step distal to IGF 1 receptors ( IGF IRs ) , as AIRs altered neither IGF 1 binding nor IGF 1 induced IGF IR autophosphorylation , but inhibited the ability of IGF IRs to mediate tyrosine phosphorylation of IRS 1 and IRS 2 in response to IGF 1 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Heightened tyrosine phosphorylation of IRS 2 during granulocytic differentiation was not caused by an increase in expression of the tyrosine kinase IGF 1 receptor , as measured by the amount of both the alpha and beta subunits . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| These effects are mediated by changes in tyrosine phosphorylation of IGF 1 receptor substrates , including IRS 2 and Shc . . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Inhibition of insulin like growth factor 1 receptor and IRS 2 signaling by ethanol in SH SY5Y neuroblastoma cells . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| It stimulated tyrosine phosphorylation of the type 1 IGF receptor ( IGF IR ) beta subunit , IRS 1 , IRS 2 , and Shc , and these changes were associated with activation of Erk and Akt . ^^^ PD 98059 inhibited activation of Erk and LY 294002 repressed activation of Akt in response to IGF 1 , but did not affect tyrosine phosphorylation of the IGF IR , IRS 1 , IRS 2 , or Shc . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Despite a significant reduction in levels of IGF 1R , IRS 1 , and IRS 2 phosphorylation , phospho p42 / p44 MAPK levels were only slightly decreased . ^^^ Estradiol induced epithelial cell proliferation was associated with inhibition of IGFBP 3 gene expression , stimulation of IGFBP 2 gene expression , and increases in IGF 1R , IRS 1 , IRS 2 , and c Raf 1 levels . ^^^ Although basal phosphorylation of IGF 1R , IRS 1 , IRS 2 , Akt 1 , and the p 85 subunit of PI 3K was significantly increased by estradiol , basal phospho p44 / 42 MAPK was significantly reduced . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylation of IGF IR , IRS 2 , Shc , and p42 / p44 MAP kinase ( MAPK ) , and the association of Grb 2 with Shc , were examined by immunoprecipitations and Western blotting . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| However , dephosphorylation of F IRS 2 significantly increased phosphorylation by the IGF 1 receptor , suggesting that basal phosphorylation of IRS 2 has divergent effects on its interaction with the insulin and IGF 1 receptors . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Igf1r haploinsufficiency did not affect insulin action and compensatory beta cell growth in insulin resistant mice with combined Insr and Igf1r heterozygous null mutations , resulting in a considerably milder phenotype than combined haploinsufficiency for Insr and its main signaling substrates , Irs 1 and Irs 2 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| IRS 2 translocates to the nuclei of mouse embryo fibroblasts expressing the IGF IR , but , at variance with IRS 1 , does not translocate in cells expressing the Simian virus 40 T antigen . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| We investigated the effects of RA on the regulation of the IGF IR and its key signaling elements : IRS 1 , IRS 2 , and SHC . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) , two major downstream molecules of IGF 1R , are known to be important in the genesis of diabetes . ^^^ Interestingly , like renal cancer cells , in AsPC 1 cells PKC zeta leads to direct Sp 1 dependent VPF / VEGF transcription ; in addition , it also promotes a negative feedback loop to IRS 2 that decreases the association of IRS 2 / IGF 1R and IRS 2 / p85 . ^^^ Taken together , our results show that in AsPC 1 pancreatic carcinoma cells , Sp 1 dependent VPF / VEGF transcription is controlled by IGF 1R signaling through IRS 2 proteins and modulated by a negative feedback loop of PKC zeta to IRS 2 . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| In conclusion , human placentas from pregnancies complicated by IUGR are characterized by decreased IGF 1 receptor content , selective impairment of the IRS 2 / phosphatidyl inositol 3 kinase pathway , and reduced p 38 and c Jun N terminal kinase activation . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| Levels of IRS 1 and IRS 2 were elevated in all tumours in the presence or absence of IGF IR , suggesting that the signalling pathway downstream of IGF IR is activated via IGF IR independent mechanisms in HCC . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| This study initially confirmed in mouse osteoblast cultures that PTH treatment increased IGF 1 mRNA and protein levels and alkaline phosphatase activity , which were accompanied by phosphorylations of IGF 1 receptor , insulin receptor substrate ( IRS ) 1 and IRS 2 , essential adaptor molecules for the IGF 1 signaling . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| IGF 1 promoted cholangiocyte proliferation in association with overexpression of p IGF1R , IRS 1 , IRS 2 , p ERK1 / 2 and p AKT . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| In contrast , tyrosine phosphorylation of IRS 2 decreased in IGF IR deficient cells ; its protein content was unchanged as compared with wild type cells . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| SH EP cells are glial like , express low levels of the type 1 IGF 1 receptor ( IGF IR ) and IRS 2 and high levels of IRS 1 . ^^^ SH SY5Y cells are neuroblast like , with high levels of IGF IR and IRS 2 but virtually no IRS 1 . ^^^ When stimulated with IGF 1 , IRS 1 expression remains constant in SH EP cells ; however , IRS 2 in SH SY5Y cells shows time and concentration dependent degradation , which requires IGF IR activation . ^^^ In summary , 1 ) IRS 2 is more sensitive to IGF 1 mediated degradation ; 2 ) IRS degradation is mediated by phosphatidylinositol 3 kinase and proteasome sensitive pathways ; and 3 ) high levels of IGF IR , and possibly the subsequent increase in Akt phosphorylation , are required for efficient IRS degradation . . ^^^ |
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| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| In these cells , the type 1 insulin like growth factor ( IGF 1 ) and granulocytic colony stimulating factor ( G CSF ) induce differentiation to granulocytes . 32D cells do not express insulin receptor substrate 1 ( IRS 1 ) or IRS 2 , docking proteins of the IGF 1 receptor . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| IRS 2 phosphorylation induced by both insulin and AspB 10 insulin was nearly insensitive to blocking the insulin receptor , being predominantly mediated by the IGF 1 receptor . ^^^ We conclude that in myoblasts IRS 2 , but not IRS 1 , functions as preferred substrate for the IGF 1 receptor . ^^^ |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y4H2 and P08069 |
Pubmed |
SVM Score :0.0 |
| NA |
|