| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.96412655 |
| CAP associates with c Cbl in 3T3 L 1 adipocytes independently of insulin stimulation in vivo and in vitro in an SH 3 domain mediated manner . 0.96412655^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.51133265 |
| Thiazolidinediones and insulin resistance : peroxisome proliferatoractivated receptor gamma activation stimulates expression of the CAP gene . c Cbl associated protein ( CAP ) is a signaling protein that interacts with both c Cbl and the insulin receptor that may be involved in the specific insulin stimulated tyrosine phosphorylation of c Cbl . 0.51133265^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.71763926 |
| Like c Cbl , Cbl b associates constitutively with CAP and interacts with Crk upon insulin stimulation . 0.71763926^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| A role for CAP , a novel , multifunctional Src homology 3 domain containing protein in formation of actin stress fibers and focal adhesions . c Cbl associated protein , CAP , was originally cloned from a 3T3 L 1 adipocyte cDNA expression library using full length c Cbl as a bait . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Molecular recognition properties of the C terminal Sh 3 domain of the Cbl associated protein , Cap . ^^^ A phage displayed combinatorial peptide library was used to define the specificity of one of the three Src homology 3 ( SH 3 ) domains in a novel cytoskeletal protein , named CAP , for Cbl Associated Protein . ^^^ Peptide sequences resembling this consensus were identified in two signal transduction proteins , c Cbl and son on sevenless ( Sos ) , previously shown to interact with the C terminal SH 3 domain of CAP . ^^^ Genetic fusion of 16 and 14 amino acid segments of c Cbl and Sos , respectively , to bacterial alkaline phosphatase confirmed that these segments were potential ligand sites for the C terminal SH 3 domain of CAP . ^^^ Alanine scanning mutagenesis of the c Cbl peptide ligand confirmed that most of the residues , which were conserved among the peptide ligands selected from the combinatorial peptide library , contributed to binding to the C terminal SH 3 domain of CAP . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cloning and characterization of a functional peroxisome proliferator activator receptor gamma responsive element in the promoter of the CAP gene . c Cbl associating protein ( CAP ) is a multifunctional signaling protein that interacts with c Cbl , facilitating the tyrosine phosphorylation of c Cbl in response to insulin . ^^^ In 3T3 L 1 adipocytes and diabetic rodents , CAP gene expression is stimulated by activators of peroxisome proliferator activator receptor gamma ( PPARgamma ) , such as thiazolidinediones ( TZDs ) , resulting in increased insulin stimulated c Cbl phosphorylation . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl is recruited to the insulin receptor by interaction with the adapter protein CAP , through one of three adjacent SH 3 domains in the carboxy terminus of CAP . ^^^ Upon phosphorylation of Cbl , the CAP Cbl complex dissociates from the insulin receptor and moves to a caveolin enriched , triton insoluble membrane fraction . ^^^ Flotillin forms a ternary complex with CAP and Cbl , directing the localization of the CAP Cbl complex to a lipid raft subdomain of the plasma membrane . ^^^ Thus , localization of the Cbl CAP complex to lipid rafts generates a pathway that is crucial in the regulation of glucose uptake . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| We previously described a pathway involving the insulin stimulated tyrosine phosphorylation of Cbl , which is recruited to the insulin receptor by the adapter protein CAP . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| On phosphorylation of Cbl , the c Cbl associated protein ( CAP ) / Cbl complex dissociates from the insulin receptor and translocates to a lipid raft membrane fraction to form a ternary complex with flotillin . ^^^ The overexpression of a CAP mutant in which the SoHo domain had been deleted ( CAPDeltaSoHo ) prevented the translocation of Cbl to lipid rafts and subsequently blocked the recruitment of CrkII and C3G . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| The above data suggest presence of a histidine containing cap shielding the Cbl binding site in TC . ^^^ The cap coordinates to certain corrinoids and , possibly , produces an incapsulated Ado radical when Cbl small middle dotAdo is bound . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| We also showed that SHIP 2 was able to coimmunoprecipitate with endogenous c Cbl protein in the absence of CAP and with the insulin receptor in CHO IR cell extracts . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Furthermore , high glucose down regulated the expression of CAP and Cbl , and insulin stimulated Cbl phosphorylation , components of an insulin signaling cascade previously characterized in adipocytes . ^^^ These data suggest that high glucose specifically inhibits insulin stimulated NO synthesis and down regulates some aspects of insulin signaling , including the CAP Cbl signaling pathway , yet this is not a result of reduced PKB mediated eNOS phosphorylation at Ser 1177 . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl associated protein ( CAP ) is an adaptor protein that plays important roles in both signal transduction and cytoskeleton rearrangement . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined whether acute exposure to insulin stimulates the tyrosine phosphorylation of Cbl and its association with Cbl associated protein ( CAP ) in muscle and adipose tissue of rats in vivo . ^^^ We report herein that insulin induces Cbl tyrosine phosphorylation and association with CAP in adipose tissue but not in muscle . ^^^ We also examined the expression and tyrosyl phosphorylation state of Cbl and CAP / Cbl association in adipose tissue of rats submitted to prolonged fasting and in monosodium glutamate ( MSG ) insulin resistant rats . ^^^ An increase in Cbl phosphorylation is observed in the fat of MSG rats , parallel with an increase in association of CAP Cbl as well as an augment in CAP and Cbl protein expression in the adipose tissue of these animals . ^^^ In adipocytes of fasted rats , there is a decrease in CAP and Cbl protein expression , insulin induced Cbl phosphorylation , and the association with CAP . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Phosphatidylinositol 3 kinase ( PI 3 K ) and the small G protein Rac govern myocyte actin remodeling , whereas TC 10 alpha contributes to adipocyte actin dynamics downstream of Cbl associated protein ( CAP ) and Cbl , independently of PI 3 K . ^^^ We found CAP expression and insulin mediated Cbl phosphorylation in differentiated myotubes but not in myoblasts . ^^^ TC 10 alpha transfected into myoblasts is activated by insulin despite the lack of CAP expression and Cbl phosphorylation . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Primary and essential role of the adaptor protein APS for recruitment of both c Cbl and its associated protein CAP in insulin signaling . ^^^ Although both APS and CAP ( c Cbl associated protein ) interact with c Cbl during insulin signaling , the relative importance of each protein in recruiting c Cbl has not been clear . ^^^ In cells co expressing insulin receptor and CAP , without APS , no association of the insulin receptor and CAP could be detected and no insulin stimulated phosphorylation of Cbl was observed . ^^^ Insulin stimulated Cbl phosphorylation was reconstituted when APS was co expressed with insulin receptor , with or without CAP . ^^^ Taken together , these results indicate that APS plays a central role in recruiting both CAP and c Cbl to the insulin receptor after insulin stimulation and is necessary and sufficient for the insulin stimulated phosphorylation of c Cbl , whereas SH 2 Balpha may provide an alternative pathway for the recruitment of CAP . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| The APS adapter protein plays a pivotal role in coupling the insulin receptor to CAP and c Cbl in the phosphatidylinositol 3 kinase independent pathway of insulin stimulated glucose transport . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| RNAi based analysis of CAP , Cbl , and CrkII function in the regulation of GLUT 4 by insulin . ^^^ To test the involvement of a PI 3 kinase independent pathway leading to activation of the TC 10 GTPase , the putative intermediates CAP , c Cbl , Cbl b , and CrkII were selectively depleted in 3T3 L 1 adipocytes using highly efficient small interfering ( si ) RNAs . ^^^ However , siRNA mediated gene silencing of both Cbl isoforms or CAP or CrkII in these cells failed to attenuate insulin stimulated deoxyglucose transport or Myc tagged GLUT 4 GFP translocation at either sub maximal or maximal concentrations of insulin . ^^^ These data are consistent with the hypothesis that CAP , Cbl iso forms , and CrkII are not required components of insulin signaling to GLUT 4 transporters . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| RNAi ( RNA interference ) based depletion of components in the putative TC 10 pathway ( CAP , CrkII and c Cbl plus Cbl b ) or the phospholipase Cgamma pathway failed to diminish insulin signalling to GLUT 4 . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| The APS adapter protein is recruited to the autophosphorylated kinase domain of the insulin receptor and initiates the phosphatidylinositol 3 kinase ( PI3K ) independent pathway of insulin stimulated glucose transport by recruiting CAP and c Cbl . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| High fat feeding effects on components of the CAP / Cbl signaling cascade in Sprague Dawley rat skeletal muscle . ^^^ The aim of this investigation was to determine whether the CAP ( Cbl associated protein ) / Cbl signaling cascade is present and responsive to insulin in skeletal muscle and if high fat feeding impairs insulin stimulated activation of this signaling cascade . ^^^ In plasma membrane fractions , neither the high fat diet nor insulin altered the insulin receptor beta subunit ( IR beta ) , APS ( adaptor protein containing PH and SH 2 domains ) , c Cbl , or TC 10 protein concentration , but high fat feeding did decrease CAP protein concentration . ^^^ APS , c Cbl , CAP , and TC 10 messenger RNA were present in the skeletal muscle and reflected the protein concentration of experimental groups . ^^^ These findings suggest that the CAP / Cbl signaling cascade is present in skeletal muscle , activated by insulin , and impaired by high fat feeding . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| In adipocytes , the Cbl / CAP dependent signaling pathway has been involved in regulating insulin stimulated glucose uptake . ^^^ Subsequent TC 10 activation was detected only in heart and adipose tissue . c Cbl and CAP gene expression was significantly reduced in the heart tissue of streptozotocin induced diabetic animals , whereas no change was observed for other components of the pathway . ^^^ In conclusion , our data demonstrate that Cbl / CAP / TC10 insulin signaling pathway is active in cardiac muscle and impaired during obesity and insulin deficiency . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| Selective modulation of the CAP / Cbl pathway in the adipose tissue of high fat diet treated rats . ^^^ In the present study , we investigated whether a HFD can modulate glucose uptake in adipose tissue by increasing signal transduction through the CAP / Cbl pathway , independently of the PI 3 K / Akt pathway . ^^^ Our results suggest that , in HFD , the differential regulation of insulin induced glucose uptake between skeletal muscle and adipose tissue may , in part , be a consequence of the CAP / Cbl / C3G pathway , since the expression of CAP and Cbl , and also the activation of this pathway were increased in adipose tissue but not in muscle . . ^^^ |
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| Interacting proteins: Q9BX66 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
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