| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.63141778 |
| Loss of BubR 1 also impairs the interaction between Mad 2 , Bub 3 , and Cdc 20 , an anaphase activator . 0.63141778^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Checkpoint inhibition of the APC / C in HeLa cells is mediated by a complex of BUBR 1 , BUB 3 , CDC 20 , and MAD 2 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Overexpression of BUB 1 , which interacts genetically with BUB 3 and which is involved in the same checkpoint pathway , also rescues the cold sensitivity of tub 1 729 , but another checkpoint gene , MAD 2 , does not . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| We show that low concentrations of nocodazole delay cell division under the control of the previously identified mitotic checkpoint genes BUB 1 , BUB 3 , MAD 1 , and MAD 2 and independently of BUB 2 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Similar to cells overexpressing MPS 1 , the BUB 1 5 delay was dependent upon the functions of the other checkpoint genes , including BUB 2 and BUB 3 and MAD 1 , MAD 2 , and MAD 3 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| We determined the chromosomal localization of it and other spindle checkpoint genes , including MAD1L1 , MAD 2 , BUB 3 , TTK ( MPS1L1 ) , and CDC 20 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Antisera to CENP A , CENP B , CENP C , CENP E , CENP F , INCENP , CLIP 170 , dynein , dynactin subunits p 150 ( Glued ) and Arp 1 , MCAK , Tsg 24 , p55CDC , HZW 10 , HBUB 1 , HBUBR 1 , BUB 3 , MAD 2 , ERK 1 , 3F3 / 2 , topoisomerase 2 and a murine HP 1 homologue , M 31 , were used in immuno fluorescence experiments in conjunction with FISH employing specific DNA probes to clearly identify neocentromeric DNA . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| We have examined the status of proteins associated with the checkpoint protein complex ( BUB 1 , BUBR 1 , BUB 3 , MAD 2 ) , the anaphase promoting complex ( Tsg 24 , p55CDC ) , and other proteins associated with mitotic checkpoint control ( ERK 1 , 3F3 / 2 epitope , hZW 10 ) , on a human dicentric chromosome . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Spindle checkpoint protein Bub 1 is required for kinetochore localization of Mad 1 , Mad 2 , Bub 3 , and CENP E , independently of its kinase activity . ^^^ Immunodepletion of Bub 1 abolishes the spindle checkpoint and the kinetochore binding of the checkpoint proteins Mad 1 , Mad 2 , Bub 3 , and CENP E . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Bub 3 interaction with Mad 2 , Mad 3 and Cdc 20 is mediated by WD 40 repeats and does not require intact kinetochores . ^^^ The kinetochore checkpoint pathway , involving the Mad 1 , Mad 2 , Mad 3 , Bub 1 , Bub 3 and Mps 1 proteins , prevents anaphase entry and mitotic exit by inhibiting the anaphase promoting complex activator Cdc 20 in response to monopolar attachment of sister kinetochores to spindle fibres . ^^^ We show here that Cdc 20 , which had previously been shown to interact physically with Mad 2 and Mad 3 , associates also with Bub 3 and association is up regulated upon checkpoint activation . ^^^ Moreover , co fractionation experiments suggest that Mad 2 , Mad 3 and Bub 3 may be concomitantly present in protein complexes with Cdc 20 . ^^^ Conversely , point mutations altering the conserved WD 40 motifs of Bub 3 , which might be involved in the formation of a beta propeller fold devoted to protein protein interactions , disrupt its association with Mad 2 , Mad 3 and Cdc 20 , as well as proper checkpoint response . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| The cold sensitivity of tub 1 729 is suppressed by extra copies of a subset of the mitotic checkpoint genes BUB 1 , BUB 3 , and MPS 1 , but not MAD 1 , MAD 2 , and MAD 3 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Upon checkpoint activation , checkpoint protein complexes containing BubR 1 ( Mad 3 ) , Bub 3 , Mad 2 and Cdc 20 directly bind to the anaphase promoting complex and inhibit its ligase activity . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| We have found that elevated levels of Ran ' s exchange factor ( RCC 1 ) abrogate the spindle assembly checkpoint in Xenopus egg extracts , restore APC / C activity , and disrupt the kinetochore localization of checkpoint regulators , including Mad 2 , CENP E , Bub 1 , and Bub 3 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| When the spindle checkpoint is activated at unattached kinetochores , the checkpoint proteins BubR 1 , Bub 3 and Mad 2 bind and inhibit Cdc 20 , an activator of the anaphase promoting complex ( APC ) . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| In the Direct Inhibition Model , the Mitotic Checkpoint Complex ( MCC ) consisting of BubR 1 , Bub 3 , Mad 2 and Cdc 20 binds and inhibits the APC / C independently of the kinetochore . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Here , we analyse how the kinetochore localization of the Drosophila spindle checkpoint proteins Bub 1 , Mad 2 , Bub 3 and BubR 1 , behave in response to alterations in microtubule binding or tension . ^^^ Altogether , our results suggest that spindle checkpoint proteins sense distinct aspects of kinetochore interaction with the spindle , with Mad 2 and Bub 1 monitoring microtubule occupancy while BubR 1 and Bub 3 monitor tension across attached kinetochores . . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| By analyzing the timing of kinetochore separation , we find that this anaphase delay requires the Bub 3 , Mad 3 , and Bub 1 but not the Mad 1 or Mad 2 spindle assembly checkpoint proteins . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Measurements of fluorescence recovery after photobleaching ( FRAP ) showed that GFP Cdc 20 and GFP BubR 1 exhibit biphasic exponential kinetics at unattached kinetochores , with approximately 50 % displaying very fast kinetics ( t1 / 2 of approximately 1 3 s ) and approximately 50 % displaying slower kinetics similar to the single exponential kinetics of GFP Mad 2 and GFP Bub 3 ( t1 / 2 of 21 23 s ) . ^^^ CONCLUSIONS : Our data support catalytic models of checkpoint activation where Mad 1 and Bub 1 are mainly resident , Mad 2 free of Mad 1 , BubR 1 and Bub 3 free of Bub 1 , Cdc 20 , and Mps 1 dynamically exchange as part of the diffuse wait anaphase signal ; and Mad 2 interacts with Cdc 20 at unattached kinetochores . . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| The WD repeats of the Bub 3 and Cdc 20 kinetochore proteins are important for the physical interactions of these proteins with Mad 2 and BubR 1 to yield a kinetochore protein complex capable of delaying anaphase by inhibiting ubiquitin ligation via the anaphase promoting complex / cyclosome . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Mps 1 overexpression in budding yeast is sufficient to trigger a mitotic arrest , which is dependent on the other mitotic checkpoint components , Bub 1 , Bub 3 , Mad 1 , Mad 2 , and Mad 3 . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Favored models of spindle checkpoint signaling propose that two inhibitory complexes ( Mad 2 Cdc20 and Mad 2 Mad3 Bub 3 Cdc20 ) must be assembled at kinetochores in order to inhibit mitosis . ^^^ The proteins Mad 2 , Mad 3 , Bub 3 , Cdc 20 , and Cdc 27 in yeast were quantified , and there are sufficient amounts to form stoichiometric inhibitors of Cdc 20 and the anaphase promoting complex . ^^^ There is a small amount of Mad 2 Mad3 Bub 3 Cdc20 and a much larger amount of a complex that contains Mad 2 Cdc20 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Moreover , Mad 2 , but not BubR 1 , Bub 1 , Bub 3 , or Mps 1 , requires Rod / Zw10 for its accumulation on unattached kinetochores . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Oocyte growth is predominantly under exogenous control , however resumption of meiotic progression is dictated by genes that influence proper chromatin and spindle regulation , such as Cdk , Histone H1oo , Fmn 2 , Mad 2 , and Bub 3 . ^^^ |
|
| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Both lack of attachment due to spindle / kinetochore defects and lack of tension across kinetochores generate the `` wait anaphase ' ' signal transmitted by the SAC , which involves the evolutionarily conserved Mad 1 , Mad 2 , Mad3 / BubR1 , Bub 1 , Bub 3 and Mps 1 proteins , and inhibits the activity of the ubiquitin ligase Cdc20 / APC , that promotes both sister chromatid dissociation in anaphase and mitotic exit . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Analysis of BubR 1 complexes suggests that both of these arms converge on the mitotic checkpoint complex ( MCC ) , which includes BubR 1 , Bub 3 , Mad 2 and Cdc 20 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| We used synthetic genetic array ( SGA ) analysis using spindle assembly checkpoint mutants mad 1 , mad 2 , mad 3 , and bub 3 . ^^^ We found 228 synthetic interactions with the four spindle assembly checkpoint mutants with substantial overlap in the spectrum of interactions between mad 1 , mad 2 , and bub 3 . ^^^ In contrast , there were many synthetic interactions that were common to mad 1 , mad 2 , and bub 3 that were not shared by mad 3 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Double mutants between mal 3 and a subset of checkpoint mutants , including bub 1 , bub 3 , mad 3 and mph 1 , but not mad 1 or mad 2 , show massive chromosome mis segregation defects . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Atb 2 983 is incorporated into the microtubules , and their overall structures are not altered noticeably , but microtubule dynamics is compromised during interphase . atb 2 983 displays a high rate of chromosome missegregation and is synthetically lethal with deletions in a subset of spindle checkpoint genes including bub 1 , bub 3 , and mph 1 , but not with mad 1 , mad 2 , and mad 3 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : We analyzed sequences and expression levels ( RNA and protein ) of eight major spindle checkpoint genes ( MAD1L1 , MAD2L1 , MAD2L2 , BUB 1 , BUB1B , BUB 3 , CDC 20 , and TTK ) in a panel of 12 breast cancer cell lines , most with established genetic instability and defective spindle damage checkpoint response . mRNA levels of these genes were also measured in primary tumor samples , and immunohistochemical staining was used to evaluate BUB1B protein levels in a panel of 270 additional cases of breast cancer . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Here , we discuss the particularities of the mouse early embryonic cell cycle and review the mutations that result in cell cycle defects during mouse early embryogenesis , including deficiencies for genes of the cyclin family ( cyclin A 2 and B 1 ) , genes involved in cell cycle checkpoints ( Mad 2 , Bub 3 , Chk 1 , Atr ) , genes involved in ubiquitin and ubiquitin like pathways ( Uba 3 , Ubc 9 , Cul 1 , Cul 3 , Apc 2 , Apc 10 , Csn 2 ) as well as genes the function of which had not been previously ascribed to cell cycle regulation ( Cdc2P1 , E4F and Omcg 1 ) . . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| This analysis predicts that cancer cells manifesting a stem cell like expression profile of a death from cancer signature would exhibit the following features : a concomitantly increased expression of certain members of inhibitor of apoptosis protein ( IAP ) family ( Survivin and XIAP ) ; activation of mitotic spindle check point proteins ( BUB 1 , BUB 3 , KNTC 2 , Mad 2 , PLK 1 , PLK 4 , STK6 / Aurora A ) ; and elevated levels of certain cell cycle control / marker proteins ( CCNB 1 , CCNB 2 , CCND 1 , CCNA 2 , CDC 2 , CDC 25 , Ki 67 , USP 22 ) . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers , mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad 1 , Mad 2 , Bub 3 , and Cdc 20 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| To prevent chromosome missegregation due to early degradation of cyclin B and securin , mitotic checkpoint protein complexes consisting of BubR 1 , Bub 3 and Mad 2 bind to and inhibit APC ( Cdc 20 ) until all chromosomes are properly attached to the mitotic spindle and aligned in the metaphase plate . ^^^ Disruption of Mad 2 , BubR 1 , Bub 3 or Rae 1 in mice results in substantial aneuploidy in somatic tissues , but whether these genes are equally important for accurate chromosome segregation during meiosis has not yet been established . ^^^ To address this issue , we generated cohorts of male mice in which Mad 2 , BubR 1 , Bub 3 , Rae 1 and Nup 98 were disrupted either individually or in combination . ^^^ We found that male fertility and accurate chromosome segregation during spermatogenesis are highly dependent on BubR 1 , but not Mad 2 , Bub 3 , Rae 1 and Nup 98 . ^^^ |
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| Interacting proteins: O43684 and Q13257 |
Pubmed |
SVM Score :0.0 |
| Molecular analyses of the mitotic checkpoint components hsMAD 2 , hBUB 1 and hBUB 3 in human cancer . ^^^ These results suggest that mutations of the hsMAD 2 , hBUB 1 and hBUB 3 genes are very rare in bladder tumors and that hsMAD 2 alterations are also infrequent in soft tissue sarcomas and hepatocellular carcinomas . . ^^^ |
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