Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.84067316
Here we examine the interactions of CaR with filamin A in HEK 293 and M 2 or A 7 melanoma cells to determine how interactions with filamin A facilitate signaling . 0.84067316^^^ Filamin A interacts with CaR through two predicted beta strands from residues 962 to 981 ; interactions between filamin A and CaR are greatly enhanced by exposure to 5 mM Ca2+ . 0.52442185^^^
Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
Analysis of truncation and deletion constructs of the CaR C terminus and the filamin cDNA clone demonstrated that the CaR and filamin interact via regions containing aa 907 997 of the CaR C terminus and aa 1566 1875 of filamin . ^^^ The functional importance of their interaction was documented by transiently expressing the CaR in M 2 melanoma cells that lack filamin , or in A 7 melanoma cells that stably express filamin , and demonstrating that the CaR activated ERK only in the presence of filamin . ^^^ Co expression of the CaR with a peptide derived from the region of the CaR C terminus that interacts with filamin reduced the ability of the CaR to activate p42ERK in a dose dependent manner , but did not inhibit the ability of the ET ( A ) receptor to activate ERK . ^^^ The fact that filamin interacts with the CaR and other cell signaling proteins including mitogen activated protein kinases and small GTPases , indicates that it may act as a scaffolding protein to organize cell signaling systems involving the CaR . . ^^^
Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
Filamin A binds to the carboxyl terminal tail of the calcium sensing receptor , an interaction that participates in CaR mediated activation of mitogen activated protein kinase . ^^^ With the use of the yeast two hybrid system , we identified filamin A ( an actin cross linking , putative scaffold protein that binds mitogen activated protein kinase ( MAPK ) components activated by the CaR ) as an intracellular binding partner of the CaR ' s carboxyl ( COOH ) terminal tail . ^^^ Moreover , confocal microscopy combined with two color immunofluorescence showed co localization of the CaR and filamin A within parathyroid cells as well as HEK 293 cells stably transfected with the CaR . ^^^ Deletion mapping localized the sites of interaction between the two proteins to a stretch of 60 amino acid residues within the distal portion of the CaR ' s COOH terminal tail and domains 14 and 15 in filamin A , respectively . ^^^ Finally , introducing the portion of filamin A interacting with the CaR into CaR transfected HEK 293 cells using protein transduction with a His tagged , Tat filamin A fusion protein nearly abolished CaR mediated activation of ERK1 / 2 MAPK but had no effect on ERK1 / 2 activity stimulated by ADP . ^^^
Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
Furthermore , we identified a novel pathway that mediates transient [ Ca ( 2+ ) ] ( 1 ) oscillations produced by the CaR in response to l phenylalanine , which requires the organization of the actin cytoskeleton and involves the small GTPase Rho , heterotrimeric proteins of the G ( 12 ) subfamily , the C terminal region of the CaR , and the scaffolding protein filamin A . ^^^
Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
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Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
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Interacting proteins: P41180 and P21333 Pubmed SVM Score :0.0
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