| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| In conclusion , the recognition of RAMP 1 and 2 as mediators of CRLR expression as a CGRP or ADM receptor has been extended , with evidence that endogenous RAMP 2 is sufficient to reveal an ADM receptor in UMR 106 cells . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The calcitonin receptor like receptor ( CRLR ) can function as either a receptor for calcitonin gene related peptide ( CGRP ) or for adrenomedullin ( ADM ) , depending upon the coexpression of a novel family of single transmembrane proteins , which we have called receptor activity modifying proteins or RAMPs . ^^^ RAMPs 1 , 2 , and 3 transport CRLR to the plasma membrane with similar efficiencies , however RAMP 1 presents CRLR as a terminally glycosylated , mature glycoprotein and a CGRP receptor , whereas RAMPs 2 and 3 present CRLR as an immature , core glycosylated ADM receptor . ^^^ Characterization of the RAMP2 / CRLR and RAMP3 / CRLR receptors in HEK293T cells by radioligand binding ( 125I ADM as radioligand ) , functional assay ( cAMP measurement ) , or biochemical analysis ( SDS polyacrylamide gel electrophoresis ) revealed them to be indistinguishable , even though RAMPs 2 and 3 share only 30 % identity . ^^^ Coexpression of RAMP2 / 1 with CRLR formed a core glycosylated ADM receptor , whereas the RAMP1 / 2 chimera generated both core glycosylated and mature forms of CRLR and enabled both ADM and CGRP receptor binding . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Differential glycosylation of human and rat ( r ) calcitonin ( CT ) receptor like receptors ( CRLR ) as a result of interactions with accessory receptor activity modifying proteins ( RAMP ) 1 or 2 was considered to define CT gene related peptide ( CGRP ) or adrenomedullin ( ADM ) receptors in mammalian cells . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The calcitonin receptor like receptor ( CRLR ) requires novel receptor activity modifying proteins ( RAMPs ) for its function as an adrenomedullin ( ADM ) or a calcitonin ( CT ) gene related peptide ( CGRP ) receptor . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA species coding for three putative ADM receptors ( the L 1 orphan receptor , RDC 1 , and calcitonin receptor like receptor ) and accessory proteins ( receptor activity modifying proteins ) were present in astrocytes . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The calcitonin receptor like receptor ( CRLR ) and the orphan receptor RDC 1 have been proposed as CGRP 1 receptors , and recent evidence suggests that CRLR can function as either a CGRP 1 receptor or an adrenomedullin ( ADM ) receptor . ^^^ Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of CRLR : coexpression of CRLR and RAMP 1 results in a CGRP 1 receptor , whereas coexpression of CRLR and RAMP 2 or 3 results in an ADM receptor . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Also , receptor activity modifying protein 2 ( RAMP 2 ) has been shown to confer ADM affinity to the pulmonary calcitonin receptor like receptor ( CRLR ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin gene related peptide ( CGRP ) and adrenomedullin ( ADM ) , two closely related peptides , initiate their biological responses through their interaction with calcitonin receptor like receptor ( CRLR ) . ^^^ Similarly characterization of RAMP2 / human or porcine CRLR and RAMP3 / human or porcine CRLR combination by radioligand binding ( [ 125I ] rADM ) and functional assay ( activation of adenylyl cyclase ) revealed the properties of ADM ( 22 52 ) sensitive ADM receptor . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a hypotensive peptide , that derives from the proteolytic cleavage of pro ( p ) ADM and acts through two subtypes of receptors , called L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ CRLR may function as a calcitonin gene related peptide or a selective ADM receptor depending on the expression of the subtype 1 or the subtypes 2 and 3 of a family of proteins , named receptor activity modifying proteins ( RAMPs ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The existence of the receptor CRLR for ADM and their associated proteins RAMP 1 and RAMP 2 was demonstrated by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The receptor for ADM , a molecular complex consisting of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 2 ( RAMP 2 ) , is activated through a unique intracellular transport mechanism . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ADM is thought to act through the G protein coupled receptor calcitonin receptor like receptor ( CRLR ) , with specificity being conferred by the receptor associated modifying protein 2 ( RAMP 2 ) . ^^^ Since Ishikawa cells express the putative ADM receptor CRLR RAMP 2 the production and secretion of ADM with the consecutive upregulation of Bcl 2 could establish an autocrine / paracrine mechanism rescuing malignant cells from hypoxic cell death . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| It has recently been shown that the molecular pharmacology of CGRP and ADM is determined by coexpression of one of three receptor activity modifying proteins ( RAMPs ) with calcitonin receptor like receptor ( CRLR ) . ^^^ As expected CRLR RAMP 1 coexpression conferred sensitivity to CGRP , whilst association of RAMP 2 or RAMP 3 with CRLR conferred high affinity ADM binding . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a hypotensive peptide , that derives from the proteolytic cleavage of pro ( p ) ADM and acts through at least two subtypes of receptors , called L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ CRLR may function as a calcitonin gene related peptide ( CGRP ) or a selective ADM receptor depending on the expression of the subtype 1 or the subtypes 2 and 3 of a family of proteins , referred to as receptor activity modifying proteins ( RAMPs ) . ^^^ RT PCR also demonstrated high levels of L 1 R mRNA and relatively low levels of CRLR mRNA , as well as the presence of specific mRNAs for the three RAMPs , thereby indicating that human adrenal cortex and aldosteronomas are provided with the two subtypes of classic ADM receptors . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a hypotensive peptide , that derives from the proteolytic cleavage of pro ( p ) ADM and acts through two subtypes of receptors , called L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ CRLR may function as a calcitonin gene related peptide or a selective ADM receptor depending on the expression of the subtype 1 or the subtypes 2 and 3 of a family of proteins , named receptor activity modifying protein ( RAMPs ) . ^^^ In conclusion our study provides evidence that 1 ) human ZG cells express ADM and ADM receptors of both L 1 and CRLR / PAMP2 , 3 subtypes ; and 2 ) through the activation of these receptors , ADM exerts an aldosterone antisecretagogue action and a growth promoting effect on cultured ZG cells , the latter an effect which includes both stimulation of proliferation and inhibition of apoptosis . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Recent researches suggest that adrenomedullin ( ADM ) and calcitonin gene related peptide ( CGRP ) bind to the same calcitonin receptor like receptors ( CRLR ) , with receptor specificity being determined by a receptor activity modifying protein ( RAMP ) . ^^^ Our objective was to explore the significance of CRLR / RAMP hypothesis in cardiovascular tissues through experiments on the phenomenon of desensitization of both ADM and CGRP receptors using cultured rat aortic vascular smooth muscle cells ( VSMCs ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of the calcitonin receptor like receptor ( CRLR ) ; co expression of RAMP 1 and CRLR results in a CGRP receptor , whereas the association of RAMP 2 or RAMP 3 with CRLR gives an ADM receptor . ^^^ No change was observed in mRNAs coding for CRLR , RAMP 2 , RDC 1 ( canine orphan receptor ) , and ADM . ^^^ The present results suggested after congestive heart failure in adult rats , an up regulation of the CGRP receptor ( by an increase in RAMP 1 that is associated with CRLR ) in atria and ventricles and of ADM receptor ( by increased RAMP 3 expression that is associated with CRLR ) in atria . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ADM acts via two subtypes of ADM ( 22 52 ) sensitive receptors : the L 1 receptor ( L 1 R ) and the calcitonin receptor like receptor ( CRLR ) , the latter behaves as selective ADM receptor only in the presence of the receptor activity modifying proteins ( RAMPs ) 2 and 3 . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Co transfection of bovine CRLR with human RAMP 2 or RAMP 3 cDNAs in HEK 293 cells displayed high affinity ADM receptors . ^^^ These observations suggest that in the absence of exogenous RAMPs heterologous expression of bovine CRLR results in an ADM receptor phenotype . . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a hypotensive peptide , which originates from the proteolytic cleavage of pro ( p ) ADM and acts via two subtypes of receptors , named L 1 R ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| In contrast to CGRP and ADM , which exhibited a preferential stimulation of CRLR when co expressed with RAMP 1 and RAMP 2 or RAMP 3 , respectively , intermedin represents a nonselective agonist for the RAMP coreceptors . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ADM binds to plasma membrane receptors composed of calcitonin receptor like receptor ( CRLR ) , a member of serpentine receptor superfamily , and receptor activity modifying protein ( RAMP ) type 2 or 3 . ^^^ ADM has also some affinity for CGR ( 1 ) receptor composed of CRLR and RAMP 1 . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ADM acts via at least two subtypes of receptors , named L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ CRLR functions as a calcitonin gene related peptide or a selective ADM receptor depending on the expression of the subtype 1 or the subtypes 2 and 3 of a family of receptor activity modifying proteins ( RAMPs ) . ^^^ In conclusion , our study provides evidence that 1 ) rat ZG cells express ADM and ADM receptor of L 1 and CRLR / RAMP2 subtypes , which both are sensitive to ADM [ 22 52 ] ; and 2 ) endogenous ADM system modulates in an autocrine / paracrine manner ZG growth , by stimulating cell proliferation and reducing cell apoptotic deletion . . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We report here on the characterization and immunohistochemical localization in human tissues of calcitonin receptor like receptor ( CRLR ) which was recently found to mediate the effects of both calcitonin gene related peptide ( CGRP ) and adrenomedullin ( ADM ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The calcitonin gene related peptide ( CGRP ) receptor and adrenomedullin ( ADM ) receptor are generated by the concomitant expression of a calcitonin receptor like receptor ( CL receptor ) and a specific receptor activity modifying protein ( RAMP ) in mammals . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a hypotensive peptide , which derives from the proteolytic cleavage of pro ( p ) ADM and acts via two main subtypes of receptors , referred to as L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ While L 1 R is selective for ADM , CRLR may bind either calcitonin gene related peptide ( CGRP ) or ADM depending on the expression of the subtype 1 or the subtypes 2 and 3 of a family of chaperones , named receptor activity modifying proteins ( RAMPs ) . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Thus , two Class 2 G protein coupled receptors , the CRLR and CTR , associate with three RAMP to form high affinity receptors for CGRP , ADM , or amylin . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Our work was aimed to explore the production of ADM , changes and pathophysiological significance of ADM mRNA and ADM receptor components calcitonin receptor like receptor ( CRLR ) and receptor activity modifying proteins ( RAMPs ) mRNA in calcified myocardium and aorta of rats induced by Vitamin D 3 plus nicotine . ^^^ The amount of ADM , CRLR and RAMPs mRNA was determined by semi quantitative RT PCR . ^^^ Furthermore , it was found that the amount of ADM , CRLR and RAMP 2 mRNA in calcified myocardium was elevated by 90 . 6 , 157 . 5 and 119 . 6 % ( all P < 0 . 01 ) , RAMP 3 mRNA was decreased by 14 . 1 % ( P < 0 . 01 ) , respectively , compared with control . ^^^ The amount of ADM , CRLR , RAMP 2 and RAMP 3 mRNA in calcified aorta was elevated by 37 . 7 % ( P < 0 . 01 ) , 41 . 4 % ( P < 0 . 01 ) , 60 . 1 % ( P < 0 . 05 ) and 13 % P < 0 . 01 ) , respectively , compared with control . ^^^ The elevated level of CRLR and RAMP 2 mRNA were in positive correlation with that of ADM mRNA ( r = 0 . 992 and 0 . 882 , respectively , P < 0 . 01 ) in calcified myocardium . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Recently , receptor activity modifying proteins ( RAMPs ) have been shown to transport calcitonin receptor like receptor ( CRLR ) to the cell surface to present either as CGRP receptor or ADM receptor . ^^^ In this work , we explored the production of ADM , alterations and significance of ADM mRNA and its receptor system components CRLR and RAMPs mRNA in calcified VSMCs . ^^^ Furthermore , it was found that the levels of ADM , CRLR , RAMP 2 and RAMP 3 mRNA in calcified cells were elevated , respectively , compared with that of control . ^^^ The elevated levels of CRLR , RAMP 2 and RAMP 3 mRNA were significant correlation with ADM mRNA ( r = 0 . 83 , 0 . 92 and 0 . 93 , respectively , all p ' s < 0 . 01 ) in calcified VSMCs . ^^^ The results show that calcified VSMCs generate an increased amount of ADM , up regulate gene expressions of ADM and its receptor system components CRLR , RAMP 2 and RAMP 3 , suggesting an important role of ADM and its receptor system in the regulation of vascular calcification . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Therefore , we examined whether mRNAs encoding CGRP or ADM receptor subunit , RDC 1 , calcitonin receptor like receptor ( CRLR ) , and receptor activity modifying proteins ( RAMPs ) are present , and if so , whether their expression is modified by IgE receptor triggering , in a mucosal type mast cell line , rat basophilic leukemia ( RBL 2H3 ) cells using RT PCR . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ADM receptors are formed by heterodimerization of the CRLR and a RAMP 2 or RAMP 3 molecule . ^^^ Since glucocorticoid responsive elements were recently identified in the human CRLR promoter and that glucocorticoids exert a major action in bones , we investigated the acute effect of dexamethasone ( Dex ) treatment on ADM receptor components in osteoblastic cell types : the MC3T3 E 1 cells and calvaria derived osteoblastic cells . ^^^ We suggest that Dex , in osteoblastic cells , altered ADM receptor by inhibition of CRLR expression and consequently could impair the ADM anabolic effect on bone . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| In this study , reverse transcription polymerase chain reaction ( RT PCR ) was used to detect the changes in mRNAs levels of preproadrenomedullin ( ppADM ) gene encoding adrenomedullin ( ADM ) and the essential receptor components of ADM , calcitonin receptor like receptor ( CRLR ) , and the receptor activity modifying protein 2 and 3 ( RAMP 2 and RAMP 3 ) in the medulla oblongata , hypothalamus , midbrain , pituitary gland and adrenal gland of the stress induced hypertensive rats . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The ADM receptor is a complex molecule that consists of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 2 ( RAMP 2 ) . ^^^ Because CRLR determines the binding specificity of ADM , the CRLR gene is thought to be a susceptibility gene of hypertension . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Our results showed that ADM receptor components the mRNA and proteins of calcitonin receptor like receptor ( CALCRL ) and receptor activity modifying proteins ( RAMPs ) were expressed by human choriocarcinoma JAr cells and first trimester cytotrophoblast HTR 8 / SV neo cells . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To explore the production of ADM , changes and significance of adrenomedullin ( ADM ) mRNA and ADM receptor system calcitonin receptor like receptor ( CRLR ) and receptor activity modifying proteins ( RAMPs ) mRNA in calcified myocardium and aorta of rats . ^^^ The amount of ADM , CRLR and RAMPs mRNA was determined by semi quantitative RT PCR . ^^^ Furthermore , it was found that the levels of ADM , CRLR and RAMP 2 mRNA in calcified myocardium were elevated by 90 . 6 % 157 . 5 % and 119 . 6 % ( all P < 0 . 01 ) , respectively , but RAMP 3 mRNA was decreased by 14 . 1 % ( P < 0 . 01 ) . ^^^ The levels of ADM , CRLR , RAMP 2 and RAMP 3 mRNA in calcified aorta were elevated by 37 . 7 % ( P < 0 . 01 ) , 41 . 4 % ( P < 0 . 01 ) , 60 . 1 % ( P < 0 . 05 ) and 13 % ( P < 0 . 01 ) respectively , compared with the control . ^^^ The elevated levels of CRLR , RAMP 2 or RAMP 3 mRNA were positively correlated with up regulations of ADM mRNA in calcified myocardium and aorta . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We further examined the expression of ADM and ADM signaling components Ramp 2 , Ramp 3 , and CRLR in the immature rat uterus and found that both E 2 and Cou regulated these genes expression . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Using highly specific ribonuclease protection analysis , mRNAs encoding three putative AM receptors [ L 1 , calcitonin receptor like receptor ( CRLR ) and RDC 1 ] were shown to be present at high concentrations in RNA extracts from lung . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Three receptors have been characterized by their ability to bind adrenomedullin ( AM ) : L 1 , RDC 1 , and CRLR . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Associated with the calcitonin receptor like receptor ( CRLR ) , RAMP 2 or RAMP 3 may function as the AM receptor . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To investigate the expression of human adrenomedullin ( ADM ) and its receptor receptor activity modifying protein 2 / calcitonin receptor like receptor ( RAMP2 / CRLR ) mRNA in the tissues of normal adrenal medulla and pheochromocytoma . ^^^ The expression of ADM and RAMP2 / CRLR mRNA were studied by reverse transcription polymerase chain reaction . ^^^ The ratios of ADM / GAPDH , RAMP2 / GAPDH , CRLR / GAPDH were used to evaluate the expression levels of ADM , RAMP 2 and CRLR mRNA . ^^^ RESULTS : Expressions of ADM and its receptor RAMP2 / CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues . ^^^ ADM / GAPDH were 0 . 48+ / 0 . 09 and 0 . 75+ / 0 . 24 , RAMP2 / GAPDH 0 . 79+ / 0 . 12 and 1 . 29+ / 0 . 30 , CRLR / GAPDH 0 . 40+ / 0 . 08 and 0 . 87+ / 0 . 22 in normal adrenal medulla and pheochromocytomas , respectively ( P < 0 . 05 ) . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| RAMP 3 is known to modify calcitonin gene related receptor ( CRLR ) so that it can then serve as an ADM receptor . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Constituents of the ADM heterodimeric receptor calcitonin receptor like receptor ( CRLR ) / receptor activity modifying protein ( RAMP ) 2 were up regulated at the mRNA and protein levels in cultured RA FLS compared with OA FLS . ^^^ ADM induced rapid intracellular cAMP production in FLS and reduced caspase 3 activity , DNA fragmentation , and chromatin condensation in RA FLS exposed to apoptotic conditions , indicating that CRLR / RAMP 2 was fully functional . ^^^ Our data suggest that ADM may prevent or reduce RA FLS apoptosis , via up regulation of its functional receptor CRLR / RAMP 2 . ^^^ Regulation of ADM secretion and / or CRLR / RAMP 2 activation may constitute new treatment strategies for RA . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Intact rat heart expressed mRNA encoding the putative AM / CGRP receptors RDC 1 and CRLR at 37 and 15 fold higher levels , respectively , than the AM selective receptor L 1 , with a qualitatively similar profile in cultured neonatal cardiac myocytes . ^^^ The high level of expression of RDC 1 and CRLR suggests that both AM and CGRP may have direct actions on the cardiac myocyte via common receptors that can interact with either ligand . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Coexpression of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein ( RAMP ) 2 or 3 have been reported to form the adrenomedullin ( AM ) specific receptor . ^^^ It is suggested that the abundant binding sites of AM in lungs are formed by CRLR and RAMP 2 in healthy subjects and that their reduction should contribute to the increase of plasma AM concentrations during sepsis . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Expression of the calcitonin receptor like receptor ( CRLR ) and its receptor activity modifying proteins ( RAMPs ) can produce calcitonin gene related peptide ( CGRP ) receptors ( CRLR / RAMP1 ) and adrenomedullin ( AM ) receptors ( CRLR / RAMP2 or 3 ) . ^^^ CRLR GFP failed to generate responses to CGRP or AM without RAMPs . ^^^ Agonist mediated internalization of CRLR GFP was observed in RAMP1 / CGRP or AM , RAMP2 / AM , and RAMP3 / AM , which occurred with similar kinetics , indicating the existence of ligand specific regulation of CRLR internalization by RAMPs . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The displacing potencies of CGRP and CGRP [ 8 37 ] were very weak . mRNAs for AM as well as calcitonin receptor like receptor and receptor activity modifying protein 2 which form a receptor specific to AM , were highly expressed in the microvessels . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM mRNA as well as calcitonin receptor like receptor mRNA and receptor activity modifying protein 2 mRNA , were highly expressed in the choroid plexus . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor / receptor activity modifying protein 2 ( CRLR / RAMP2 ) and CRLR / RAMP3 complexes have been reported to be specific adrenomedullin ( AM ) receptors . ^^^ Renal gene expressions of AM , CRLR , RAMP 2 , and RAMP 3 were measured at 2 weeks ( decompensated phase ) and 5 weeks ( compensated phase ) after the operation . ^^^ At 5 weeks after the operation , there were no differences in mRNA levels of AM , CRLR , RAMP 2 , and RAMP 3 between the 2 groups . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The receptor activity modifying proteins ( RAMPs ) and the calcitonin receptor like receptor ( CRLR ) are both required to generate adrenomedullin ( AM ) and calcitonin gene related peptide ( CGRP ) receptors . ^^^ In contrast , RAMP 2 and 3 promoted the expression of smaller , core glycosylated , CRLR forms , which were linked to AM receptor pharmacology . ^^^ Since core glycosylation is classically a trademark of immature proteins , we tested the hypothesis that the core glycosylated CRLR forms the AM receptor . ^^^ Although significant amounts of core glycosylated CRLR were produced upon co expression with RAMP 2 or 3 , cross linking experiments revealed that ( 125 ) 1 AM only bound to the fully glycosylated forms . ^^^ Our results also show that the three RAMPs lie close to the peptide binding pocket within the CRLR RAMP heterodimers , since ( 125 ) 1 AM and ( 125 ) 1 CGRP were incorporated in RAMP 2 , 3 , and 1 , respectively . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM and AM mRNA were detected in KG1C cells by immunohistochemistry and RT PCR . mRNAs for calcitonin receptor like receptor ( CRLR ) and receptor activity modifying proteins ( RAMPs ) 1 , 2 and 3 but not for calcitonin receptors were detected in the cells , while mRNAs for CRLR , calcitonin receptors and all RAMPs were detected in the human cerebellum . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| In this review we have focused on the proposition that the calcitonin receptor like receptor ( CRLR ) is a receptor both for AM and the related vasoactive peptide calcitonin gene related peptide ( CGRP ) . ^^^ We will describe how AM receptors have been reported to signal and be regulated and to consider whether further receptors for AM beyond CRLR / RAMP combinations might exist . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The calcitonin receptor like receptor ( CRLR ) can function as a receptor for either calcitonin gene related peptide ( CGRP ) or adrenomedullin ( AM ) , depending upon co expression with members of a novel family of receptor activity modifying proteins ( RAMP ) . ^^^ RAMP 1 presents the CRLR at the cell surface as a CGRP / AM receptor . ^^^ RAMP 2 and RAMP 3 transported CRLR receptors act as AM specific receptors . ^^^ The pattern of differential and cell specific expression of CRLR and RAMP suggests the involvement of CRLR / RAMP1 in the processes of vasodilation , smooth muscle relaxation and angiogenesis in response to AM and CGRP in the human uterus , but also indicates that other receptors may be implicated . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Recently , calcitonin receptor like receptor ( CRLR ) and receptor activity modifying proteins ( RAMPs ) have been shown to function together to serve as AM receptors stimulating cAMP production . ^^^ In the present study , we examined the effects of ET on AM secretion , intracellular cAMP response to AM , and gene expressions of CRLR and RAMPs in cultured cardiac myocytes . ^^^ AM increased the intracellular cAMP level in a dose dependent manner and the cAMP accumulation by AM was significantly amplified by 24 h preincubation with ET 1 . 10 nmol / L ET 1 significantly increased the CRLR mRNA level without any effect on RAMP 1 mRNA . 1 micromol / L ET 1 significantly reduced the RAMP 2 mRNA level , but ET 1 dose dependently increased the RAMP 3 mRNA level in the cardiac myocytes . ^^^ These findings suggest that ET 1 not only stimulates AM secretion , but also modulates intracellular cAMP responses to AM probably by altering the expressions of CRLR and RAMPs in rat cardiomyocytes . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| When co expressed with a receptor activity modifying protein ( RAMP ) accessory protein , calcitonin receptor like receptor ( CRLR ) can function as a calcitonin gene related peptide receptor ( CRLR RAMP 1 ) or an adrenomedullin ( AM ) receptor ( CRLR RAMP2 / 3 ) . ^^^ Co expression of chimeric RAMPs and CRLR in HEK 293 cells revealed that residues 77 101 , situated in the extracellular N terminal domain of human RAMP 2 ( hRAMP 2 ) , were crucial for selective AM evoked cAMP production . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The discovery of receptor activity modifying proteins ( RAMP ) 1 and 2 and their co expression with an orphan receptor , calcitonin receptor like receptor ( CRLR ) has led to the elucidation of functional CGRP and AM receptors , respectively . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| It is possible that changes in newly reported AM receptors calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 2 or 3 ( RAMP 2 , RAMP 3 ) as well as AM binding protein 1 ( AMBP 1 ) may also play distinct roles in the biphasic cardiovascular response observed during sepsis . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Transient transfection of cardiomyocytes using a cAMP responsive element ( CRE ) luciferase reporter gene as an indirect measure of cAMP activation , demonstrated that overexpression of either CRLR or RAMP 2 potentiated the AM signalling response . ^^^ Overexpression of CRLR and RAMP 2 together led to an additive effect on AM signalling that was approximately 4 fold greater than control cardiomyocytes stimulated with AM . ^^^ Furthermore , the AM mediated induction of CRE luciferase activity was abolished by coincubation with the receptor antagonist CGRP ( 8 37 ) or by overexpression of a CRLR antisense construct . ^^^ These data demonstrate that AM action in the rat cardiomyocyte requires CRLR and RAMP 2 to constitute functional AM receptors . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM receptors comprise the calcitonin receptor like receptor ( CRLR ) and a family of receptor activity modifying proteins ( RAMPs 1 3 ) ; however , the pathophysiological role of AM and its receptor system in the kidney remains to be clarified . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM receptor complexes are composed of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 2 ( RAMP 2 ) , or CRLR and RAMP 3 . ^^^ Expression of AM , RAMP 2 , RAMP 3 and CRLR mRNA was studied in the remnant kidney of rats with renal mass ablation using competitive quantitative RT PCR techniques . ^^^ In normal rat kidneys , expression levels of RAMP 2 , RAMP 3 , CRLR and AM mRNAs were 26 . 5 + / 1 . 9 mmol / mole of GAPDH , 7 . 7 + / 0 . 9 mmol / mole of GAPDH , 3 . 6 + / 0 . 2 mmol / mole of GAPDH and 0 . 57 + / 0 . 03 mmol / mole of GAPDH ( mean + / SE , n = 6 ) , respectively . ^^^ The present study showed that expression of mRNAs encoding AM , RAMP 2 , RAMP 3 and CRLR were differentially regulated in remnant kidneys of rats with renal mass ablation . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Co expression of CRLR with RAMP 1 or 2 revealed CGRP or AM receptors , respectively . ^^^ There , heterodimeric complexes between CRLR or CTR and the corresponding RAMP reveal high affinity receptors for AM , CGRP , and amylin . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We investigated circulating levels of proadrenomedullin N 20 peptide ( PAMP ) and AM , and left ventricular expression of preproAM and calcitonin receptor like receptor ( CRLR ) mRNA . ^^^ Unlike AM , the CRLR is not transcriptionally upregulated in severe CHF . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We investigated the role of the AM receptor ( AMR ) and the calcitonin gene related peptide type 1 receptor ( CGRP1R ) in regulating pulmonary vascular AM levels . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Together with human RAMP 3 , CRLR defines an AM receptor , and with mouse RAMP 3 it is a low affinity CGRP / AM receptor . ^^^ Thus amylin and CGRP interact specifically with heterodimeric complexes between CTR and RAMP 1 or RAMP 3 , and CGRP and AM interact with complexes between CRLR and RAMP 1 , RAMP 2 or RAMP3 . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Three receptor activity modifying proteins ( RAMP ) define specific interactions between calcitonin ( CT ) gene related peptide ( CGRP ) , adrenomedullin ( AM ) and amylin , and a CT receptor or a CT receptor like receptor ( CRLR ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM was released into the conditioned medium of adult perineural fibroblasts ( 1749+ / 629 pgeq / 10 ( 5 ) cells per 24 h ) . mRNA encoding AM was also predominantly expressed in adult perineural fibroblasts . mRNA encoding the calcitonin receptor like receptor ( CRLR ) and the receptor activity modifying proteins ( RAMP ) 1 , 2 and 3 were demonstrated in all the primary cells , but the levels of RAMP 1 mRNA relative to 18s rRNA were 10 fold lower than those of CRLR and RAMP 2 and 3 encoding mRNA . ^^^ The results are consistent with the expression of CRLR / RAMP2 and CRLR / RAMP3 heterodimeric AM receptors in all the primary cells examined . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Two receptors , L 1 and calcitonin receptor like receptor ( CRLR ) , which are able to bind AM , have been cloned and characterized . ^^^ IR for the CRLR revealed a similar expression pattern to that seen for AM . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) gains its bioactivity by amidation at its C terminal , forming `` mature AM . ' ' The mature AM and the expression of AM receptor component mRNAs , receptor activity modifying protein 2 and calcitonin receptor like receptor , from feto maternal tissues of normal pregnant women and women with histologic chorioamnionitis were examined to clarify the pathophysiological features of this intrauterine infection . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Furthermore , gene expression of AM receptor system components such as calcitonin receptor like receptor ( CRLR ) , receptor activity modified protein 2 ( RAMP 2 ) , and RAMP 3 were significantly increased at the stage of LVH and HF . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| When coexpressed with receptor activity modifying protein ( RAMP ) 2 or 3 , calcitonin receptor like receptor ( CRLR ) functions as an adrenomedullin ( AM ) receptor ( CRLR / RAMP2 or 3 ) . ^^^ Coexpression of rat ( r ) CRLR with rRAMP deletion mutants in HEK293T cells revealed that deletion of residues 93 99 from rRAMP 2 or residues 58 64 from rRAMP 3 significantly inhibits high affinity [ 125I ] AM binding and AM evoked cAMP production , despite full cell surface expression of the receptor heterodimer . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Gene expression of AM was measured by Northern blot analysis and gene expression of AM receptor system components , such as calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein 2 ( RAMP 2 ) , and RAMP 3 was measured by the reverse transcription polymerase chain reaction method . ^^^ The gene expression levels of AM , CRLR , RAMP 2 , and RAMP 3 in the LV were significantly higher in D SHR than in the other two groups . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The AM receptor , a complex formed by calcitonin receptor like receptor ( CRLR ) and receptor activity modifying proteins ( RAMPs ) , and the recently discovered AM binding protein , complement factor H ( fH ) , are expressed in the insulin producing beta cells . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) is a hypotensive peptide , which derives from the proteolytic cleavage of pro ( p ) AM , and acts through two subtypes of receptors , named L 1 receptor ( L 1 R ) and calcitonin receptor like receptor ( CRLR ) . ^^^ CRLR functions as either a calcitonin gene related peptide ( CGRP ) receptor or a selective AM receptor depending on which member of a family of receptor activity modifying proteins ( RAMPs ) is expressed : RAMP 1 generates CGRP receptors , while RAMP 2 and RAMP 3 produce AM receptors . ^^^ In conclusion , our study demonstrated that ( 1 ) immature rat thymus cortex expresses AM and the AM receptors L 1 R and CRLR / RAMP ; and ( 2 ) AM , acting via AM ( 22 52 ) sensitive receptors , exerts a potent growth promoting effect on immature rat thymus , by enhancing proliferation and lowering apoptotic death of thymocytes . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| When co expressed with receptor activity modifying protein ( RAMP ) 1 , calcitonin receptor like receptor ( CRLR ) can function as a receptor for both calcitonin gene related peptide ( CGRP ) and adrenomedullin ( AM ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Among them , AM and CGRP are reported to share a core receptor , the calcitonin receptor like receptor ( CRLR ) , and the specificity of the CRLR is determined by the expression levels of receptor activity modifying proteins ( RAMPs ) . ^^^ The specificity of CRLR was clearly determined by the expression of RAMPs ; RAMP 1 converted CRLR to CGRP receptor , while RAMP 2 and RAMP 3 converted it to AM receptor , but the affinity of CTR for AMY was not increased by the expression of any known RAMPs . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| When co expressed with receptor activity modifying protein ( RAMP ) 2 , calcitonin receptor like receptor ( CRLR ) functions as an adrenomedullin ( AM ) receptor ( CRLR / RAMP2 ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| It has been shown that the calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein ( RAMP ) act together to function as AM receptors , although in this regard there are a number of issues , including the cellular mechanism of AM actions , that remain to be addressed . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) has two specific receptors formed by the calcitonin receptor like receptor ( CL ) and receptor activity modifying protein ( RAMP ) 2 or 3 . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We also measured gene expression of AM and ANP was and gene expression and protein levels of AM receptor system components such as calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein ( RAMP ) 2 and RAMP 3 . ^^^ The mRNA levels of AM , CRLR , and RAMP 2 in the LV were significantly higher in SHR SP than in WKY . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CRLR ) , a seven domain transmembrane protein functions as CGRP A receptor , in association with receptor activity modifying protein ( RAMP ) 1 , a single domain transmembrane protein , whereas CRLR and RAMP 2 or RAMP 3 constitute a receptor for AM . ^^^ Calcitonin receptor like receptor ( CRLR ) , a seven domain transmembrane protein functions as CGRP A receptor , in association with receptor activity modifying protein ( RAMP ) 1 , a single domain transmembrane protein , whereas CRLR and RAMP 2 or RAMP 3 constitute a receptor for AM . ^^^ In the present investigation , we examined the mRNA expression of CRLR , RAMP 1 , RAMP 2 , and RAMP 3 in rat uterus ( n = 8 ) by reverse transcriptional analysis and polymerase chain reaction to assess the changes in the expression of CGRP A and AM receptor components during pregnancy and labor and by steroid hormone treatments in adult ovariectomized rats . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| In this study , we examined whether Ang 2 affects the gene expression of the AM receptor components of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein ( RAMP ) in rat cardiomyocytes . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The expressions of AM and its receptor CRLR mRNA on cultured glomerular epithelial cells ( GEC ) and MsC were investigated by Northern blot assay , and the possible effect of AM secreted by GEC on MsC proliferation was observed using [ 3H ] thymidine incorporation as an index . ^^^ Northern blot assay showed that AM mRNA was expressed only on cultured GEC , but not on MsC , however , AM receptor CRLR mRNA was only expressed on MsC . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) has two known receptors formed by the calcitonin receptor like receptor ( CL ) and receptor activity modifying protein ( RAMP ) 2 or 3 : we report the effects of the antagonist fragments of human AM and CGRP ( AM 22 52 and CGRP 8 37 ) in inhibiting AM at human ( h ) , rat ( r ) and mixed species CL / RAMP2 and CL / RAMP3 receptors transiently expressed in Cos 7 cells or endogenously expressed as rCL / rRAMP2 complexes by Rat 2 and L 6 cells . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Since adrenomedullin ( AM ) elicits vasodilatation by binding to specific AM receptors consisted of calcitonin receptor like receptor ( CRLR ) / receptor activity modifying protein 2 ( RAMP 2 ) or CRLR / receptor activity modifying protein 3 ( RAMP 3 ) on endothelial cells and stimulating nitric oxide production , AM possibly involves in glomerular capillary dilatation in early phase of diabetic nephropathy . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| CGRP , AMY and AM receptors are complexes which include a GPCR ( the CT receptor or CTR and calcitonin receptor like receptor or CRLR ) and a single transmembrane domain protein known as receptor activity modifying proteins ( RAMPs ) as well as an intracellular protein named receptor component protein ( RCP ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM and its receptor component [ calcitonin receptor like receptor and receptor activity modifying protein 2 ( CRLR / RAMP2 ) ] mRNAs were expressed in a variety of rat adipose tissues , including epididymal , mesenteric , retroperitoneal , and subcutaneous adipose tissue . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| ELT 3 cells expressed CGRP and AM receptor components CRLR , RAMP 1 , RAMP 2 , and RAMP 3 . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM 2 increased cAMP accumulation in cells expressing human CT receptor like receptor ( CRLR ) and one of receptor activity modifying proteins ( RAMPs ) , but it was no more potent than CGRP and AM . ^^^ There remains a possibility that a new AM 2 specific receptor or an additional RAMP that enables CRLR to be an AM 2 specific receptor , exists in mammals . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| We measured AM mature , active form , and AM total ( active form+inactive form ) in plasma and renal tissues , and mRNA levels of AM and AM receptor system components such as calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein ( RAMP ) 2 , and RAMP 3 in renal tissues . ^^^ In addition , mRNA levels of AM in the renal cortex and medulla and the mRNA levels of CRLR , RAMP 2 , and RAMP 3 in the renal cortex were higher in SHR SP than in WKY . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) is a hypotensive peptide , that acts via the calcitonin receptor like receptor ( CRLR ) , whose interaction with the subtypes 2 and 3 of a family of receptor activity modifying proteins ( RAMP ) gives rise to two distinct AM receptors , named AM 1 and AM 2 receptors . ^^^ Taken together , our findings allow us to suggest that : 1 ) endogenous AM system plays an important autocrine paracrine growth promoting action in the human prostate , being possibly involved in the development of the malignant phenotype of epithelial cells ; and 2 ) the tumor promoting effect of AM in the human prostate is mainly mediated by the AM 2 receptor ( CRLR / RAMP3 ) subtype . . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CRLR ) is a seven transmembrane ( 7 TM ) domain class B G protein coupled receptor ( GPCR ) which requires coexpression of different receptor activity modifying proteins ( RAMP ) to become a functional calcitonin gene related peptide ( CGRP ) receptor or an adrenomedullin ( AM ) receptor . ^^^ Calcitonin receptor like receptor ( CRLR ) is a seven transmembrane ( 7 TM ) domain class B G protein coupled receptor ( GPCR ) which requires coexpression of different receptor activity modifying proteins ( RAMP ) to become a functional calcitonin gene related peptide ( CGRP ) receptor or an adrenomedullin ( AM ) receptor . ^^^ The ability of Nt CRLR to bind CGRP and AM independent of RAMPs was determined by studying inhibition of ( 125 ) 1 CGRP and ( 125 ) 1 AM binding to pregnant rat uterine membrane in the presence of Nt CRLR protein . ^^^ We observe that Nt CRLR inhibits ( 125 ) 1 CGRP and ( 125 ) 1 AM binding to rat uterus in a dose dependent fashion ( IC ( 50 ) = 0 . 25 and 0 . 29 muM , respectively ) . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| A functional receptor for the vasodilatory ligand , adrenomedullin ( AM ) , is comprised of RAMP 2 or RAMP 3 and calcitonin receptor like receptor ( CRLR ) . ^^^ However , in the presence of NHERF 1 , although the AM receptor ( CRLR / RAMP3 ) undergoes desensitization , the internalization of the receptor complex is blocked . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Both AM and its receptor , calcitonin receptor like receptor , are upregulated through a hypoxia inducible factor 1 dependent pathway under hypoxic conditions . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Co localization of adrenomedullin ( AM ) and its receptor components such as calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein ( RAMP ) 2 and RAMP 3 in peripheral tissues , including the heart , kidney , and vasculature , suggests an important role for the peptide as a regulator of cardiovascular function . ^^^ Recently , we also found that not only levels of AM peptide and AM gene expression , but also mRNA levels of CRLR , RAMP 2 and RAMP 3 are increased in cardiac hypertrophy and failing heart . ^^^ Cardiac myocytes and fibroblast produce and secrete two molecular forms of AM and express CRLR , RAMP 2 and RAMP 3 , and AM is known to have inhibitory effect of collagen synthesis and antiproliferative effect in cardiac fibroblasts . ^^^ |
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| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| CGRP and AM share a G protein coupled core receptor called calcitonin receptor like receptor ( CLR ) , and the specificity of the CLR is determined by the interaction with receptor activity modifying proteins ( RAMPs ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM acts via the calcitonin receptor like receptor ( CLR ) interacting with a receptor activity modifying protein ( RAMP ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Receptor activity modifying proteins ( RAMPs ) enable calcitonin receptor like receptor ( CRLR ) to function as a calcitonin gene related peptide receptor ( CRLR / RAMP1 ) or an adrenomedullin ( AM ) receptor ( CRLR / RAMP2 or 3 ) . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| AM exerts its effects via the calcitonin receptor like receptor ( CRLR , now known as CL ) complexed with either receptor activity modifying protein ( RAMP ) 2 or 3 . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CL ) is a G protein coupled receptor ( GPCR ) that mediates effects of AM , but little information is available on its expression and functional state in human tumors . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CRLR , now known as CL ) can function as either an AM receptor or a CGRP receptor , when cotransfected with receptor activity modifying proteins ( RAMPs ) that define ligand binding specificity . ^^^ Calcitonin receptor like receptor ( CRLR , now known as CL ) can function as either an AM receptor or a CGRP receptor , when cotransfected with receptor activity modifying proteins ( RAMPs ) that define ligand binding specificity . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| The Calcrl / embryos exhibit extreme hydrops fetalis and cardiovascular defects , including thin vascular smooth muscle walls and small , disorganized hearts remarkably similar to the previously characterized AM / phenotype . ^^^ In vivo assays of cellular proliferation and apoptosis in the hearts and vasculature of Calcrl / and AM / embryos support the concept that AM signaling is a crucial mediator of cardiovascular development . ^^^ The Calcrl gene targeted mice provide the first in vivo genetic evidence that CLR functions as an AM receptor during embryonic development . . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( AM ) and calcitonin gene related peptide ( CGRP ) have structural similarities , interact with each others receptors ( calcitonin receptor like receptor ( CLR ) / receptor activity modifying proteins ( RAMPs ) ) and show overlapping biological activities . ^^^ |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and Q16602 |
Pubmed |
SVM Score :0.0 |
| NA |
|