| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Mitogenesis of MC and MAPK activity when stimulated to a similar extent by endothelin ( ET 1 ) was inhibited by ADM to a significantly ( P < 0 . 01 ) lesser degree ( 30 % ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Production and secretion of endothelin 1 ( ET 1 ) and adrenomedullin ( ADM ) by a cultured human colorectal adenocarcinoma cell line , DLD 1 , were studied by radioimmunoassay and Northern blot analysis . ^^^ Northern blot analysis showed the expression of ET 1 mRNA and ADM mRNA in the DLD 1 cells . ^^^ This study has shown the production and secretion of two vasoactive peptides , ET 1 and ADM , by DLD 1 colorectal adenocarcinoma cells . ^^^ These findings suggest possible pathophysiologic roles for ET 1 and ADM in colon mucosal epithelial cells and tumors derived from them . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In all hypertensive patients plasma ADM concentrations where not correlated with blood pressure , plasma renin activity , plasma endothelin 1 , 2 , or plasma aldosterone . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Hopefully such manipulation of ADM and ET 1 can result in further improvement in the well being of heart failure patients . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| The study reported here therefore set out to examine these two functions for two vasoactive peptides , endothelin 1 ( ET 1 ) and adrenomedullin ( ADM ) in SW 13 human adrenocortical carcinoma cells by radioimmunoassay and Northern blot analysis . ^^^ Northern blot analysis showed the expression of ET 1 and ADM mRNAs in SW 13 cells . ^^^ This study has shown that ET 1 and ADM are the two unique vasoactive peptides that are produced and secreted by adrenocortical carcinoma cells . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Among neuropeptides with local pulmonary artery pressor effects are endothelin 1 ( ET 1 ) , angiotensin 2 ( AII ) , and substance P , and among mitigating peptides are calcitonin gene related peptide ( CGRP ) , adrenomedullin ( ADM ) , atrial natriuretic peptide ( ANP ) , vasoactive intestinal peptide ( VIP ) and ET 3 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Exogenous ET 1 ( 1 to 100 nM ) more potently stimulated the release of ADM from ET ( A ) / ET ( B ) compared with ET ( A ) isolates . ^^^ Possession of ET ( A ) / ET ( B ) receptors is associated with higher rates of ADM release and slower proliferation , but a capacity for ET 1 stimulated DNA synthesis via ET ( A ) receptors . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin ( ADM ) is a potent vasodilator and reverses the vasoconstrictor action of endothelin 1 ( ET 1 ) . ^^^ These studies aimed to determine the effect of ADM on ET 1 synthesis in bovine aortic endothelial cells ( BAEC ) and to identify the possible mechanisms involved . ^^^ ADM caused a potent concentration dependent inhibition of ET 1 release that was correlated with reduced preproET 1 mRNA levels . ^^^ However , a 100 fold discrepancy between the threshold ADM concentration for cyclic AMP production and inhibition of ET 1 release was observed . ^^^ ADM caused a more marked reduction in stimulated ET 1 synthesis with a threshold of 1 pM , and suppression of ET 1 release to basal levels at 100 nM . 8 Bromo cyclic AMP , showed no concentration dependent inhibition of ET 1 release , yet caused a 50 % reduction in TNFalpha stimulated intercellular adhesion molecule 1 ( ICAM 1 ) mRNA levels . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Then , accumulation of vasoactive peptides sensitive to hypoxia including endothelin 1 ( ET 1 ) , vascular endothelial growth factor ( VEGF ) , and adrenomedullin ( ADM ) was measured . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| ET 1 gene expression was significantly reduced ( > 50 % ) after ADM aerosol therapy ( p < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| At the peaks of submaximal contractions to norepinephrine ( NE ) or endothelin ( ET 1 ) , rings were exposed to 5 10 10 ( 6 ) M acetylcholine ( ACh ) or 9 10 10 ( 9 ) M adrenomedullin ( ADM ) or 1 . 3 10 10 ( 8 ) M calcitonin gene related peptide ( CGRP ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| The changes of adrenomedullin ( ADM ) , endothelin 1 ( ET 1 ) and nitric oxide ( NO ) levels before and after operation in congenital heart disease ( CHD ) associated with pulmonary hypertension ( PH ) were observed in order to investigate their role in CHD with PH and their clinical significance . ^^^ Plasma ADM , ET 1 and NO levels were determined by radioimmunoassay and colorimetry methods . ^^^ The correlation between ADM and ET 1 , NO , PASP was analyzed . ^^^ The changes in plasma ADM , ET 1 and plasma NO on the 7th day after operation among the groups were compared . ^^^ The results showed that plasma ADM levels in non PH group were significantly higher than that in control group ( P < 0 . 05 ) , but there was no significant difference in ET 1 and NO levels between the two groups ( P > 0 . 05 ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVES : To investigate the expression of endothelin 1 ( ET 1 ) and adrenomedullin ( ADM ) in the renal pelvis , stenotic ureteropelvic junction , and ureter of 20 male Wistar rats with congenital unilateral ureteropelvic junction obstruction ; the normal contralateral kidneys served as controls . ^^^ Our study group has recently been able to demonstrate a significant increase in ET 1 and a significant decrease in ADM in prestenotic and stenotic tissue , but not in the remainder of the ureter , compared with controls . ^^^ Total RNA was extracted , and subsequently 1 microg of RNA was reversely transcribed . mRNA expression of ET 1 and ADM was determined semiquantitatively using on line polymerase chain reaction . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Endothelin 1 , a potent pulmonary vasoconstrictor , is regulated by ADM via cAMP . ^^^ Twenty two hours after inhalation of aerosolised ADM , endothelin 1 mRNA in lung tissue and endothelin 1 protein expression in pulmonary arteries was reduced compared with controls ( median semi quantitative immunhistochemical score : ADM 0 . 21 , control 0 . 76 ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To investigate if the vasoactive systems adrenomedullin ( ADM ) and endothelin 1 ( ET 1 ) are expressed in human adipose tissues in children and in adults and to determine the distribution pattern of nitric oxide synthases ( NOS ) . ^^^ MEASUREMENTS : ADM , ET 1 , the endothelial ( eNOS ) and inducible ( iNOS ) NOS as well as two housekeeping genes were measured using quantitative real time PCR . ^^^ CONCLUSION : Human adipose tissue expresses many vasoactive substances including ADM and ET 1 . ^^^ In adults , the amounts of ET 1 and ADM as well as eNOS and iNOS mRNA are higher , possibly due to a physiological upregulation with increasing age . ^^^ Although there are differences depending on the locations of the tissues , the expression patterns of the antagonists ADM and ET 1 are quite similar , indicative of a well balanced pattern of local gene expression in normotensive individuals with normal body weight . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Intracellular Mg2+ concentrations were measured with the fluorescent dye mag fura 2 acetyoxymethylester ( AM ) and intracellular Ca2+ concentrations with the fluorescent dye fura 2AM under basal conditions and after stimulation by angiotensin 2 , saralasin ( angiotensin 2 antagonist ) , arginine vasopressin and endothelin 1 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In order to investigate the interaction of endothelin 1 ( ET 1 ) and tumor necrosis factor alpha ( TNF alpha ) and their activity in the pathogenesis of asthma , ET 1 plasma and BALF levels were measured , and the amount of TNF alpha released from alveolar macrophage ( AM ) was also tested . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| METHODS : Endothelin 1 and 3 , plasma renin activity , atrial natriuretic peptide , noradrenaline and mean arterial pressure were measured at 11 pm , 7 am , 9 am and 6 pm in 10 patients with pre ascitic cirrhosis and in 10 healthy subjects on normal sodium diet and carrying on their usual activities ( supine from 10 pm to 7 am , standing and mobile after 7 am ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| AM have been known to express prepro ET 1 mRNA . ^^^ The analysis using high pressure liquid chromatography revealed that AM derived ET consists of ET 1 , ET 2 , and ET 3 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| However , the comparative prognostic power of endothelin 1 ( Et 1 ) , adrenomedullin ( Adm ) and tumour necrosis factor alpha ( TNF alpha ) in this patient group is unknown . ^^^ Blood samples for NT proBNP , Et 1 , Adm and TNF alpha analysis were taken at recruitment and patients followed up for a median of 666 days . ^^^ The median values for NT proBNP , Et 1 , Adm and TNF alpha were 1494 pg / ml [ interquartile range 530 3930 ] , 0 . 39 fmol / ml [ 0 . 10 1 . 24 ] , 94 pg / ml [ 54 207 ] and 2 . 0 pg / ml [ 0 18 . 5 ] respectively . ^^^ CONCLUSION : A single measurement of NT proBNP in patients with advanced CHF can help identify patients at the highest risk of death , and is a better prognostic marker than Et 1 , Adm and TNF alpha . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| It was investigated in volunteers whether the ET 1 and ADM concentration in plasma changes by exercise . ^^^ There were used standard RIA kits to measure ADM and ELISA method to determine ET 1 concentration in plasma . ^^^ Average ET 1 and ADM levels in plasma were different before and after the exercise , the standard exercise test significantly decrease ET 1 and ADM concentration in plasma . ^^^ The lack of stimulating ET 1 influence on secretion of ADM can be the effect of the decrease of ADM concentration . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Thrombin , vasoactive intestinal polypeptide and interferon gamma also inhibited AM production , while angiotensin 2 , endothelin 1 , bradykinin , substance P , adrenaline , phorbol ester and fetal calf serum stimulated AM production in VSMC . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Ir AM was accumulated linearly for up to 48 hours in the culture medium of rat ECs , and the secretion rate of AM was almost comparable to that of endothelin 1 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Conditioned medium ( CM ) from rat adherent AM decreased the ppET 1 mRNA levels in ATII cells and reduced ET 1 concentrations in cell culture supernatants . ^^^ We conclude that rat AM inhibit ET 1 production by rat ATII cells in vitro through IL 1 beta secretion . ^^^ Downregulation of ET 1 production by activated AM could limit the intra alveolar burden of this profibrogenic peptide and thus could prevent fibrosis development . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Since monocytes and macrophages are thought to be activated in SSc , we hypothesized that alveolar macrophages ( AM ) and their precursors blood monocytes from patients with SSc produced more ET 1 than cells from healthy subjects . ^^^ These results show that AM from patients with SSc are hyperresponsive to LPS in vitro in terms of ET 1 and big ET 1 production and suggest that AM could participate in vivo in the overproduction of this potentially profibrotic mediator in patients with SSc . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Transforming growth factor beta 1 and FCS suppressed AM secretion but stimulated endothelin 1 ( ET 1 ) secretion . ^^^ Thyroid hormone and phorbol ester increased AM and ET 1 secretion but to a lesser extent . ^^^ Regulation of AM production in EC is found to be similar to that of VSMC with several exceptions , but AM and ET 1 production in EC are deduced to be controlled independently and by different mechanisms . ^^^ Based on these findings , AM production in EC is thought to be regulated by a variety of substances coming from blood and neighboring cells , and the secreted AM is deduced to dilate blood vessels as an endothelium derived relaxing factor competing with ET 1 . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In this study we examined the effect of LPS on the gene expression of endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) , two major vasoactive peptides predominantly produced by vascular endothelial cells , to investigate their role in the pathophysiology of septic shock . ^^^ LPS induced ET 1 and AM gene expression in the heart , lung , kidney , liver , and aorta within 6 h . ^^^ In the liver , whereas basal ET 1 and AM mRNA were hardly detectable , ET 1 and AM gene expression and peptide production were markedly increased by LPS . ^^^ This LPS induced upregulation of ET 1 and AM expression is greatly potentiated by D galactosamine ( D GalN ) , although D GalN alone could not induce ET 1 and AM gene expression . ^^^ These results , together with the previous findings that liver injury induced by LPS and D GalN is mainly mediated by tumor necrosis factor alpha ( TNF alpha ) , suggest that the LPS cytokine pathway may cause upregulation of ET 1 and AM production , leading to dysregulation of systemic and regional vascular tone . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Levels of vasoactive peptides such as adrenomedullin ( AM ) , endothelin 1 ( ET 1 ) , C type natriuretic peptide ( CNP ) , and nitric oxide ( NO ) are affected by fluid shear stress . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) are potent vasoconstrictive and vasodilative peptides . ^^^ Endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) are potent vasoconstrictive and vasodilative peptides . ^^^ We investigated effect of thiopental , ketamine , etomidate , midazolam and propofol on the production of ET 1 and AM in cultured rat aorta vascular smooth muscle cells . ^^^ Rat cultured vascular smooth muscle cells are used and the dose dependent effect ( 10 ( 8 ) 10 ( 4 ) M ) of thiopental , ketamine , etomidate , midazolam , and propofol on the production of ET 1 and AM was examined . ^^^ These results suggest that thiopental , ketamine , etomidate , propofol and midazolam differentially affect the production of ET 1 and AM in rat vascular smooth muscle cells . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| To elucidate the involvement of ET 1 in lung interstitial pathologic events , we assessed ET 1 secretion by alveolar macrophages ( AM ) and fibroblasts recovered from the bronchoalveolar lavage ( BAL ) of patients with idiopathic pulmonary fibrosis ( IPF ) , sarcoidosis ( SA ) and from control subjects . ^^^ The AM recovered from IPF patients showed increased spontaneous secretion of ET 1 compared with cells from SA and control subjects . ^^^ Moreover , neutralization of ET 1 by monoclonal antibody was shown to decrease 3H thymidine incorporation in fibroblasts cultured with AM supernatants . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In addition , 10 ( 6 ) mol / l of angiotensin 2 ( Ang 2 ) and endothelin 1 ( ET 1 ) significantly increased the AM secretion by 55 and 48 % , respectively . ^^^ Synthetic AM significantly reduced 10 ( 6 ) mol / l Ang 2 or 10 ( 7 ) mol / l ET 1 stimulated [ 3H ] thymidine and [ 3H ] phenylalanine incorporation in a dose dependent manner , and these effects were attenuated by a calcitonin gene related peptide ( CGRP ) type 1 receptor antagonist , CGRP ( 8 37 ) . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The objectives of the present study were ( 1 ) to determine whether lysophosphatidylcholine ( lyso PC ) , a prominent component of oxidatively modified LDL , induces migration of human coronary artery smooth muscle cells ( SMCs ) and , if so , to clarify the mechanism , and ( 2 ) to investigate the possible interactions of lyso PC and platelet derived growth factor ( PDGF ) BB , endothelin ( ET 1 ) , adrenomedullin ( AM ) , or vitamin E on SMC migration by the Boyden ' s chamber method . ^^^ Lyso PC induced migration was significantly enhanced by PDGF BB or ET 1 but was clearly inhibited by human AM and vitamin E . ^^^ CONCLUSIONS : These results indicate that ( 1 ) lyso PC induces human coronary artery SMC migration at least in part through release of endogenous bFGF and ( 2 ) this lyso PC induced migration can be further induced by PDGF BB and ET 1 and can be inhibited by human AM and vitamin E . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| To determine the extent to which chronic AM overproduction affects circulatory physiology under normal and pathological conditions , we used a preproendothelin 1 promoter to establish transgenic mouse lines overexpressing AM in their vasculature . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| We characterize herein the impact of myocardial nitric oxide ( NO ) synthesis on the inotropic response to two cardioactive peptides , endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) . ^^^ Impact of NO on ET 1 and AM induced inotropic responses : potentiation by combined administration . ^^^ In the isolated perfused rat heart preparation , intracoronary infusion of AM ( 0 . 03 and 1 nmol / l ) and ET 1 ( 0 . 08 and 1 nmol / l ) for 30 min induced a dose dependent , gradual increase in developed tension , the maximal responses being equal . ^^^ Inhibition of myocardial NO synthase ( NOS ) by N ( omega ) nitro L arginine methyl ester ( L NAME ; 300 micromol / l ) enhanced the inotropic response to ET 1 at a concentration of 1 nmol / l ; meanwhile , the effect of AM was not augmented significantly . ^^^ The inotropic response to simultaneous administration of low , equipotent doses of AM ( 0 . 03 nmol / l ) and ET 1 ( 0 . 08 nmol / l ) was significantly smaller than that of either peptide alone . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In order to study the roles of vasoactive peptides during tissue repair of gastric ulcers , we compared concentrations in tissue surrounding gastric ulcers of endothelin 1 ( ET 1 ) , adrenomedullin ( AM ) , and transforming growth factor beta ( TGF beta ) among different stages of ulcer development . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To investigate the maturation of the paracrine system ' s endothelial nitric oxide synthase ( eNOS ) , inducible NOS ( iNOS ) , endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) in human placenta during the 2nd and 3rd trimester of pregnancy . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| AM reverses pressor response to ET 1 independently of NO in rat coronary circulation . ^^^ The present study was designed to see whether AM can oppose the vasoconstrictor response to ET 1 . ^^^ In the presence of L NAME , AM ( 0 . 03 to 1 nmol / l ) markedly reversed the pressor response to ET 1 at both concentrations . ^^^ Our results indicate that AM may serve as a paracrine modulator of ET 1 induced vasoconstriction independently of the NO pathway . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Synthetic ET 1 dose dependently increased AM secretion from the cardiomyocytes . ^^^ AM increased the intracellular cAMP level in a dose dependent manner and the cAMP accumulation by AM was significantly amplified by 24 h preincubation with ET 1 . 10 nmol / L ET 1 significantly increased the CRLR mRNA level without any effect on RAMP 1 mRNA . 1 micromol / L ET 1 significantly reduced the RAMP 2 mRNA level , but ET 1 dose dependently increased the RAMP 3 mRNA level in the cardiac myocytes . ^^^ These findings suggest that ET 1 not only stimulates AM secretion , but also modulates intracellular cAMP responses to AM probably by altering the expressions of CRLR and RAMPs in rat cardiomyocytes . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| The objective of this study was to determine concentration of adrenomedullin ( AM ) in follicular fluid and whether a correlation exists between AM and nitric oxide ( NO ) or endothelin 1 ( ET 1 ) levels in follicular fluid , serum 17beta estradiol or other parameters of ovarian function in spontaneous and gonadotrophin stimulated ovarian cycles . ^^^ AM , ET 1 , and NO were detected in all of the follicular fluid samples and their concentrations were similar in spontaneous and stimulated cycles . ^^^ Similarly , no relationship was observed between ET 1 , NO , and AM follicular fluid concentrations in either spontaneous or stimulated cycles . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| To investigate circadian variation in ischemic threshold in chronic coronary heart disease ( CHD ) and its relation to plasma endothelin 1 ( ET 1 ) , 21 patients with stable angina underwent treadmill exercise tests twice within a day , performed at 8 9 AM for the first test and at 3 4 PM for the second one . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| We measured mature AM , atrial natriuretic peptide ( ANP ) , endothelin 1 , nitric oxide , cyclic guanosine 3 ' , 5 ' monophosphate , and catecholamine levels at 1 hour intervals during HD sessions . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Humoral factors , such as angiotensin 2 ( Ang 2 ) and endothelin 1 ( ET 1 ) , and mechanical stress due to pressure and volume overload to the heart have been shown to be involved in AM expression of the myocardium in both in vitro and in vivo studies . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| At 8 am , on the second day after admission and before treatment with amlodipine blood samples were taken from examined patients to determine plasma levels of IL 6 , TNF alpha , NPY and ET 1 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Hence , we have investigated by immunocytochemistry ( ICC ) and autoradiography the acute effects of ET 1 on endogenous AM system of adult rat heart . ^^^ Quantitative densitometry showed that ET 1 [ 1 31 ] , but not ET 1 [ 1 21 ] , significantly decreased AM IR in coronary vessels , thereby suggesting that ET 1 elicits AM release in the heart through the activation of ETA receptors . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Hence , we investigated whether endothelin 1 ( ET 1 ) and adrenomedullin ( AM ) , two regulatory peptides exerting growth promoting action on several cell systems , favor the in vitro expansion of CB SCs in liquid culture . ^^^ CB hematopoietic cell middle term expansion was carried out in a stroma free liquid culture medium in the presence of ET 1 , AM and three different cytokine combinations . ^^^ Neither ET 1 nor AM ( 2 . 5 10 10 ( 8 ) M ) were per se able to significantly increase the CFU number , but both peptides magnified the pro expansive effects of some cytokine cocktails . ^^^ In light of these findings , we conclude that ET 1 and AM are to be considered novel promising molecules that , in association with cytokines , can be utilized as pro expansive factors of CB SCs in prevision of their clinical use in allogeneic transplantation . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| When AM mRNA levels in isolated neonatal rat myocytes treated for 48 h with Ang 2 , ET 1 or PHE were measured , only Ang 2 induced a consistent increase in AM gene expression . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Specifically , AA carriers of the ET 1 polymorphism with poor AM skills exhibited the greatest TPRI reactivity . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| In vascular smooth muscle cells from ZO , ET 1 induced elevation of myoplasmic intracellular free calcium concentration ( [ Ca2+ ] 1 ) ( measured by fluo 4 AM fluorescence ) , and maximal contractions were diminished compared with ZL , both in the presence and absence of l NAME . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| ET 1 immunoreactivity was found mainly in AM of sarcoidosis BALF . ^^^ CONCLUSIONS : Increased levels of ET 1 in AM could enhance fibrogenesis in pulmonary sarcoidosis . . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Mitogenic peptide endothelin 1 increased and specific ET 1 antagonist BQ 123 and significantly reduced AM binding . ^^^ Production of AM in HeLa cells markedly increased after exposure to hypoxia > ET 1 > PMA . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| Classically described as a potent vasodilator , natriuretic , and diuretic agent , experimental infarct models also demonstrate AM to exhibit antiproliferative and antiapoptotic functions in the myocardium , counterregulating the effects of mediators such as angiotensin 2 and endothelin 1 . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| SW 13 cells express either ET 1 and AM or growth factors , and several cytokines stimulate ET 1 and AM release from SW 13 cells . ^^^ Reverse transcription polymerase chain reaction showed the expression of pro ET 1 and proAM genes , as well as detected the mRNAs of only the ET and AM receptor subtypes , which are currently thought to mediate the growth promoting action of these peptides : i . e . the ETA and AM 2 receptors . ^^^ In keeping with these observations , both ET 1 and AM markedly stimulated the growth of SW 13 cells , by enhancing the proliferation and lowering the apoptosis rate . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| The number of AM storing cells underwent a dramatic decrease in response to the potent vasoconstrictor ET 1 , suggesting an acute release of stored vasodilatory AM aimed at counteracting coronary constriction . ^^^ |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35318 and P05305 |
Pubmed |
SVM Score :0.0 |
| NA |
|