Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We reported previously that microglial cells release substantial amounts of an Abeta degrading protease that , after purification , is indistinguishable from insulin degrading enzyme ( IDE ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Recently , insulin degrading enzyme ( IDE ) , a 110 kDa metalloendopeptidase , was found to degrade both endogenously secreted and synthetic Abeta peptides . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Recent studies suggest that insulin degrading enzyme ( IDE ) in neurons and microglia degrades Abeta , the principal component of beta amyloid and one of the neuropathological hallmarks of Alzheimer ' s disease ( AD ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Recent reports suggest that insulin degrading enzyme ( IDE ) and neutral endopeptidase ( NEP ) are involved in the extracellular degradation of A beta . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Pretreatment of the medium with insulin degrading enzyme , which degrades Abeta monomers but not oligomers , did not prevent the inhibition of LTP . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We investigated the effect of Abeta peptides on insulin binding to the insulin receptor because it is known that ( 1 ) Abeta and insulin are both amyloidogenic peptides sharing a common sequence recognition motif , ( 2 ) Abeta and insulin are substrates for the same insulin degrading enzyme , and ( 3 ) impaired glucose metabolism is a characteristic event in the pathology of AD . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Secreted Abeta peptides become either degraded by the metalloproteases insulin degrading enzyme ( IDE ) and neprilysin or metabolized through receptor uptake mediated by apolipoprotein E . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
The fact that insulin and Abeta appear to share a common system for degradation and disposal as they are both substrates of the insulin degrading enzyme ( IDE ) suggested the possibility of a reciprocal interference . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) has been implicated as a candidate enzyme responsible for the degradation and clearance of Abeta in the brain . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Two substrates of insulin degrading enzyme ( IDE ) , amyloid beta protein ( Abeta ) and insulin , are critically important in the pathogenesis of Alzheimer ' s disease ( AD ) and type 2 diabetes mellitus ( DM 2 ) , respectively . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) can hydrolyze Abeta wild type , yet whether IDE is capable of degrading Abeta bearing pathogenic substitutions is not known . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Whilst there has been considerable progress made in understanding the generation of Abeta by alpha , beta and gamma secretase activity on APP , recently enzymes involved in the degradation of Abeta have been identified including neprilysin and insulin degrading enzyme ( IDE ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Liquid chromatography / mass spectrometric analysis shows that FAbetaB is hydrolyzed at closely similar sites as wild type Abeta by neprilysin and insulin degrading enzyme , the two most widely studied Abeta degrading proteases . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Within the region , the gene for insulin degrading enzyme ( IDE ) represents a notable biological candidate given that it degrades amyloid beta protein ( one of the major constituents of senile plaques ) and the intracellular amyloid precursor protein ( APP ) domain released by gamma secretase processing . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Here we show that transgenic overexpression of insulin degrading enzyme ( IDE ) or neprilysin ( NEP ) in neurons significantly reduces brain Abeta levels , retards or completely prevents amyloid plaque formation and its associated cytopathology , and rescues the premature lethality present in amyloid precursor protein ( APP ) transgenic mice . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
RNA interference experiments in Abeta transfected cells identified the protease of the proteasome independent pathway as insulin degrading enzyme ( IDE ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We found that diet induced insulin resistance promoted amyloidogenic beta amyloid ( Abeta ) Abeta 1 40 and Abeta 1 42 peptide generation in the brain that corresponded with increased gamma secretase activities and decreased insulin degrading enzyme ( IDE ) activities . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Plasmin , neprilysin , endothelin converting enzyme and insulin degrading enzyme ( IDE ) have each been implicated in A beta degradation ; data supporting the role of the latter three enzymes have included increased levels of endogenous murine A beta in mice genetically deficient for the respective enzyme . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
By using immunoprecipitation and mass spectrometry , we show that insulin degrading enzyme ( IDE ) from isolated human brain microvessels was capable of degrading ( 125 ) 1 insulin and cleaved Abeta ( 1 40 ) wild type and the genetic variants Abeta A21G ( Flemish ) , Abeta E22Q ( Dutch ) , and Abeta E22K ( Italian ) at the predicted sites . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) is one of the proteins that has been demonstrated to play a key role in degrading beta amyloid ( Abeta ) monomer in vitro and in vivo , raising the possibility of upregulating IDE as an approach to reduce Abeta . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Although the proteolytic processes leading to Abeta formation have been extensively studied , less is known about the proteases that degrade Abeta , which include insulin degrading enzyme ( IDE ) and neprilysin ( NEP ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme , encoded by IDE , plays a primary role in the degradation of amyloid beta protein ( A beta ) , the deposition of which in senile plaques is one of the defining hallmarks of Alzheimer ' s disease ( AD ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) has been identified as a candidate protease in the clearance of amyloid delta ( Abeta ) peptides from the brain . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Deletion of insulin degrading enzyme ( IDE ) in mice causes accumulation of cerebral amyloid beta protein ( Abeta ) , hyperinsulinemia , and glucose intolerance . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Levels of full length APP , its C terminal fragment , and Abeta degrading enzymes ( insulin degrading enzyme and neprilysin ) in APP / CCL2 and APP mice were indistinguishable . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
This effect was independent of mRNA / protein changes in neprilysin and insulin degrading enzyme and , instead , may involve neuronal metabolism changes that are known to affect APP processing and to be regulated by exercise . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
A strong positional and biological candidate is the gene encoding the insulin degrading enzyme ( IDE ) , a protease involved in the catabolism of Abeta . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Previous studies found an association of DS with 48C / T presenilin 1 and with the 850 tumor necrosis factor alpha , two polymorphisms of genes involved in amyloid beta modulation In this study , we evaluated whether the insulin degrading enzyme ( IDE ) , a protease involved in the degradation of endogenous brain derived Abeta peptides , is involved in DS related AD . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We have developed yeast as an expression and genetic system for functional studies of the insulin degrading enzyme ( IDE ) , which cleaves and inactivates certain small peptide molecules , including insulin and the neurotoxic A beta peptide . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Finally , using in vitro cellular models of amyloid precursor protein ( APP ) processing and Abeta generation / clearance , we confirmed that human recombinant ( hr ) CTGF may increase Abeta 1 40 and Abeta 1 42 peptide steady state levels , possibly through a mechanism that involves gamma secretase activation and decreased insulin degrading enzyme ( IDE ) steady state levels in a MAP kinase ( MAPK ) / phosphatidylinositol 3 kinase ( PI 3K ) / protein kinase B ( AKT ) 1 dependent manner . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Abeta is produced continuously and its concentration is determined in part by the activities of several degradative enzymes , including NEP ( neprilysin ) , IDE ( insulin degrading enzyme ) , ECE 1 ( endothelin converting enzyme 1 ) and ECE 2 , and probably plasmin . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) is a 110 kDa thiol zinc methalloendopeptidase that can cleave small Abeta peptides and the APP intracellular domain ( AICD ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin and IGF 1 may modulate brain levels of insulin degrading enzyme , which would also lead to an accumulation of Abeta amyloid . . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) is expressed in the brain and may play an important role there in the degradation of the amyloid beta peptide ( Abeta ) . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
To investigate these possibilities , APP tg mice were treated for 6 months with Cerebrolysin and analyzed in the water maze , followed by RNA , immunoblot , and confocal microscopy analysis of full length ( FL ) APP and its fragments , beta secretase ( BACE 1 ) , and Abeta degrading enzymes [ neprilysin ( Nep ) and insulin degrading enzyme ( IDE ) ] . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) , a zinc metalloprotease , can specifically recognize and degrade insulin , as well as several amyloidogenic peptides such as amyloid beta ( Abeta ) and amylin . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
The Abeta 1 42 peptide is generated through cleavage of the amyloid precursor protein by beta and gamma secretase and is catabolised by a variety of proteolytic enzymes such as insulin degrading enzyme and neprilysin . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We show that nicastrin deficiency drastically lowers neprilysin expression , membrane bound activity and mRNA levels , but it did not modulate the expression of two other putative Abeta cleaving enzymes , endothelin converting enzyme and insulin degrading enzyme . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
The activities of other Abeta degrading enzymes , insulin degrading enzyme and neprilysin , were unchanged . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We further report here that rosiglitazone attenuated reductions in insulin degrading enzyme ( IDE ) mRNA and activity , and reduced amyloid beta peptide ( Abeta ) 42 levels without affecting amyloid deposition , in the brains of Tg 2576 mice . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Abeta production is mediated by beta and gamma secretase activities ; it is prevented by alpha secretase activity , and insulin degrading enzyme ( IDE ) catabolizes Abeta . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
It was proposed that insulin degrading enzyme ( IDE ) participates in the clearance of amyloid beta ( Abeta ) in the brain , and its low expression or activity may be relevant for the progression of Alzheimer disease . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
To further study the action of insulysin on Abeta peptides recombinant rat insulysin was used . ^^^ Using a rat cortical cell system , the action of insulysin on Abeta ( 1 40 ) and Abeta ( 1 42 ) was shown to eliminate the neurotoxic effects of these peptides . ^^^ Insulysin was further shown to prevent the deposition of Abeta ( 1 40 ) onto a synthetic amyloid . ^^^ Taken together these results suggest that the use of insulysin to hydrolyze Abeta peptides represents an alternative gene therapeutic approach to the treatment of Alzheimer ' s disease . . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulysin has been identified as one of several proteases potentially involved in Abeta degradation based on its hydrolysis of Abeta peptides in vitro . ^^^ In this study , in vivo levels of brain Abeta 40 and Abeta 42 peptides were found to be increased significantly ( 1 . 6 and 1 . 4 fold , respectively ) in an insulysin deficient gene trap mouse model . ^^^ A 6 fold increase in the level of the gamma secretase generated C terminal fragment of the Abeta precursor protein in the insulysin deficient mouse also was found . ^^^ In mice heterozygous for the insulysin gene trap , in which insulysin activity levels were decreased approximately 50 % , brain Abeta peptides were increased to levels intermediate between those in wild type mice and homozygous insulysin gene trap mice that had no detectable insulysin activity . ^^^ These findings indicate that there is an inverse correlation between in vivo insulysin activity levels and brain Abeta peptide levels and suggest that modulation of insulysin activity may alter the risk for Alzheimer ' s disease . . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
It has recently been shown that Abeta ( 1 40 ) is cleaved by Escherichia coli pitrilysin , a homologue of insulysin , at a specific site . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Degradation of both endogenous and synthetic Abeta at picomolar to nanomolar concentrations was completely inhibited by the competitive IDE substrate , insulin , and by two other IDE inhibitors . ^^^ Immunodepletion of conditioned medium with an IDE antibody removed its Abeta degrading activity . ^^^ In addition to its ability to degrade Abeta , IDE activity was unexpectedly found be associated with a time dependent oligomerization of synthetic Abeta at physiological levels in the conditioned media of cultured cells ; this process , which may be initiated by IDE generated proteolytic fragments of Abeta , was prevented by three different IDE inhibitors . ^^^ We conclude that a principal protease capable of down regulating the levels of secreted Abeta extracellularly is IDE . . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We have developed an inhibitor of AP P called apstatin ( 1 ) ( N [ ( 2S , 3R ) 3 amino 2 hydroxy 4 phenyl butanoyl ] L prolyl L prolyl L al aninam ide ) ; IC 50 , human = 2 . 9 microM . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
A 110 kDa protein , corresponding to the molecular mass of IDE , was affinity labeled with either 125I insulin , 125I Abeta 1 40 or 125I A ( beta ) 1 42 and both A ( beta ) degradation and cross linking were specifically inhibited by an excess of each peptide . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Therefore our data demonstrate that IDE , which is one of the proteases implicated in the removal of extracellular Abeta , also removes the cytoplasmic product of gamma secretase cleaved APP . . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We recently found that homozygous deletion of the insulin degrading enzyme ( IDE ) gene in mice results in an early and marked elevation of cerebral Abeta . ^^^ For IDE to remain a valid candidate gene for late onset AD on functional grounds , it must be shown that partial loss of function of IDE can still alter Abeta degradation , but without causing early , severe elevation of brain Abeta . ^^^ Here , we show that naturally occurring IDE missense mutations in a well characterized rat model of type 2 diabetes mellitus ( DM 2 ) result in decreased catalytic efficiency and a significant approximately 15 to 30 % deficit in the degradation of both insulin and Abeta . ^^^ Endogenously secreted Abeta ( 40 ) and Abeta ( 42 ) are significantly elevated in primary neuronal cultures from animals with the IDE mutations , but there is no increase in steady state levels of rodent Abeta in the brain up to age 14 months . ^^^ We conclude that naturally occurring , partial loss of function mutations in IDE sufficient to cause DM 2 also impair neuronal regulation of Abeta levels , but the brain can apparently compensate for the partial deficit during the life span of the rat . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
Insulin degrading enzyme ( IDE ) is a protease that degrades insulin and the beta amyloid ( Abeta ) peptide implicated in Alzheimer ' s disease ( AD ) . ^^^ Hence , factors that influence IDE expression or IDE activity toward Abeta are potentially relevant to the etiology of AD . ^^^ For example , insulin , a competitive inhibitor of IDE activity toward Abeta , may be elevated in non epsilon 4 cases . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
In addition , many studies have identified a possible role of IDE in the degradation of amyloid beta protein and the intracellular amyloid precursor protein ( APP ) domain released by gamma secretase processing . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
The PLAU _ 1 associations were independent of the associations we found among plasma Abeta 42 , LOAD and variants in the IDE or VR 22 region . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
We review the current evidence , which suggests that hyperinsulinaemia may elevate Abeta through insulin ' s competition with Abeta for IDE . ^^^ The deficiency of IDE can be caused by genetic variation or by the diversion of IDE from the metabolism of Abeta to the metabolism of insulin . ^^^
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA
Interacting proteins: P14735 and P05067 Pubmed SVM Score :0.0
NA