| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.64883938 |
| We demonstrate that the SH 2 domains of SHP 2 bind directly to tyrosyl phosphorylated GHR from GH treated cells . 0.64883938^^^ |
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| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| Half the individuals with Noonan syndrome carry a heterozygous mutation of the nonreceptor type protein tyrosine phosphatase , Src homology region 2 domain phosphatase 2 ( SHP 2 ) , encoded by PTPN 11 , which has a role in GH receptor signaling . ^^^ |
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| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| As assessed specifically by anti SHP 2 coimmunoprecipitation and by affinity precipitation with a glutathione S transferase fusion protein incorporating the SH 2 domains of SHP 2 , GH induced formation of a complex of tyrosine phosphoproteins including SHP 2 , GHR , JAK 2 , and a glycoprotein with properties consistent with being a SIRP alpha like molecule . ^^^ A reciprocal binding assay using IM 9 cells as a source of SHP 1 and SHP 2 revealed specific association of SHP 2 ( but not SHP 1 ) with a glutathione S transferase fusion incorporating GHR cytoplasmic domain residues 485 620 , but only if the fusion was first rendered tyrosine phosphorylated . ^^^ GH dependent tyrosine phosphorylation of SHP 2 was also observed in murine 32D cells ( which lack IRS 1 and 2 ) stably transfected with the GHR . ^^^ Finally , GH induced transactivation of a c Fos enhancer driven luciferase reporter in GHR and JAK 2 transfected COS 7 cells was significantly reduced when a catalytically inactive SHP 2 mutant ( but not wild type SHP 2 ) was coexpressed ; in contrast , expression of a catalytically inactive SHP 1 mutant allowed modestly enhanced GH induced transactivation of the reporter in comparison with that found with expression of wild type SHP 1 . ^^^ |
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| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| This same pattern in both cells of IRS 1 dependent augmentation and IRS 1 independent wortmannin sensitivity was also observed for GH induced activation of Akt and MEK 1 ( using state specific antibody blotting for both ) , despite the lack of difference in GHR , JAK 2 , SHP 2 , p 85 , Akt , Ras , Raf 1 , MEK 1 , ERK 1 , or ERK 2 abundance between the two cells . ^^^ |
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| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10912 and Q06124 |
Pubmed |
SVM Score :0.0 |
| NA |
|