Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.72185865
In this cell system , STAT 5 directly interacts with the GHR in a GH dependent manner . 0.72185865^^^ Therefore , the GH dependent interaction of a particular STAT 5 with tyrosine phosphorylated GHR may play an important role in GH mediated signal transduction . . 0.56626721^^^ Additionally , GH induced tyrosine phosphorylation of STAT 5 and the interaction of STAT 5 with GHR can be observed in mouse 3T3 F442A cells which express endogenous mouse GHR . 0.55298207^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Activation of Stat 5 by GH was , however , abolished in cells expressing the GH receptor lacking intracellular tyrosines . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Western blotting indicates that GH also activates STAT 5 in human embryonic kidney cells ( 293 ) , which stably express the rabbit GH receptor . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Furthermore , in COS 7 cells transiently transfected with STAT 5 and GH receptor cDNAs , it was found that expression of STAT 5 was necessary for GH induction of these two DNA binding complexes . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
It has been previously demonstrated that growth hormone ( GH ) stimulated tyrosine phosphorylation of Jak 2 and Stat5a and Stat5b occurs in FDP C 1 cells expressing either the entire GH receptor or truncations of the cytoplasmic domain expressing only the membrane proximal 80 amino acids . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The role of GH receptor tyrosine phosphorylation in Stat 5 activation . ^^^ Similarly , these GH receptors were found to be able to mediate activation of Stat 5 DNA binding activity , whereas the GH receptor mutant lacking all intracellular tyrosines was not . ^^^ Synthetic tyrosine phosphorylated peptides corresponding to the GH receptor sequence around the three tyrosines inhibited Stat 5 DNA binding activity while their non phosphorylated counterparts were ineffective . ^^^ Tyrosine phosphorylated GST GH receptor fusion proteins specifically bound to Stat 5 in extracts from COS 7 cells transfected with Stat 5 cDNA . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Indeed , the dwarfism , elevated plasma GH , low plasma insulin like growth factor 1 , and development of obesity seen in STAT5b / mice are all characteristics of Laron type dwarfism , a human GH resistance disease generally associated with a defective GH receptor . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In this paper , we demonstrate ( in COS cells expressing rat GH receptor ( rGHR ) and either Stat5A or Stat5B , 3T3 F442A fibroblasts , and CHO cells expressing rGHR ) that GH induces tyrosyl phosphorylation of both Stat5A and Stat5B . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
BRL cells expressing the GH receptor were transiently transfected with expression plasmids containing either the hGH or the bGH gene and the response of the cell was measured by CAT reporter plasmids requiring either STATs 1 and 3 or STAT 5 for their response . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Continuous exposure to GH of BRL 4 cells , a rat hepatoma cell line stably transfected with rat GH receptor , induces a rapid but transient activation of Jak 2 and Stat 5 . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH inhibition of PPARalpha activity required GH receptor and STAT5b and was not observed using GH activated STAT 1 in place of STAT5b . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Increased STAT5b DNA binding activity was observed in cells treated with the proteasome inhibitor MG 132 , suggesting that at least one component of the GH receptor ( GHR ) JAK 2 STAT5b signaling pathway becomes labile in response to continuous GH treatment . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In multiple cell types , laser scanning confocal imaging of GFP Stat5B co expressed with GH receptor shows that GFP Stat5B undergoes a rapid , dramatic accumulation in the nucleus upon GH stimulation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
All four SOCS proteins were able to bind to a tyrosine phosphorylated glutathione S transferase GH receptor fusion protein , and SOCS 3 required the same 46 C terminal amino acids for GH receptor binding as it did for inhibition of GH mediated transcription and STAT 5 activation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The finding of endotoxin inhibition of in vivo STAT 5 tyrosine phosphorylation in response to a supramaximal dose of GH in the absence of a change in GH receptor abundance or total GH stimulated JAK 2 tyrosine phosphorylation provides the first demonstration of acquired postreceptor GH resistance . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Mutation of the SHP 2 binding site in growth hormone ( GH ) receptor prolongs GH promoted tyrosyl phosphorylation of GH receptor , JAK 2 , and STAT5B . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Physiological plasma GH pulses are required to obtain the high levels of activated STAT5b seen in the livers of males , and down regulation of the GH receptor ( GHR ) JAK STAT5b pathway in hepatocytes exposed to GH in a near continuous fashion underlies the low level of liver STAT5b activity that is characteristic of adult female rats . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Growth hormone ( GH ) inducible suppressors of cytokine signaling ( SOCS / CIS proteins ) inhibit GH receptor ( GHR ) signaling to STAT5b via phosphotyrosine dependent binding interactions with the tyrosine kinase JAK 2 ( SOCS 1 ) and / or the cytoplasmic tail of GHR ( CIS and SOCS 3 ) . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Furthermore , the expression vector for the GH receptor also activated the ADH promoter , and this effect was abrogated by mutations of the adjacent STAT5b and C / EBP beta binding sites . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH induced the expression of liver Igf 1 mRNA in hypophysectomized male wild type , but not in hypophysectomized male Stat5b ( / ) mice , although the Stat5b ( / ) mice exhibit both normal liver GH receptor expression and strong GH induction of Cytokine inducible SH 2 protein ( Cis ) , which is believed to contribute to the down regulation of GH induced liver STAT5b signaling . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The effects of continuous high GH levels on GH signal transduction through the GH receptor ( GHR ) / Janus kinase 2 ( JAK 2 ) / signal transducer and activator of transcription 5 ( STAT 5 ) pathway as well as the desensitization of this pathway by suppressors of cytokine signaling ( SOCS ) were studied in transgenic mice overexpressing GHRH . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH stimulated tyrosine phosphorylation of the GH receptor , signal transducer and activator of transcription 3 ( Stat 3 ) , and Stat 5 . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The exogenous expression of the GH receptor in one family of LS fibroblasts ( H 1 ) but not the other ( M ) restores signaling to a STAT 5 reporter element . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
We have reported that TNF alpha suppresses hepatic GH receptor ( GHR ) gene expression , whereas the cytokine inducible SH 2 containing protein 1 ( Cis ) / suppressors of cytokine signaling ( Socs ) genes are upregulated by TNF alpha and IL 6 and inhibit GH activation of STAT 5 . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
To investigate the mechanism involved , we studied the effects of estrogen on GH signaling through Janus kinase ( JAK ) 2 and the signal transducers and activators of transcription ( STATs ) in HEK 293 cells stably expressing the GH receptor ( 293GHR ) , HuH 7 ( hepatoma ) and T 47D ( breast cancer ) cells . 293GHR cells were transiently transfected with an estrogen receptor alpha expression plasmid and luciferase reporters with binding elements for STAT 3 and STAT 5 or the beta casein promoter . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The expression of MUP 2 is known to be stimulated by growth hormone ( GH ) , through the GH receptor ( GHR ) , Janus kinase 2 ( JAK 2 ) and signal transducer and activator of transcription 5 ( STAT 5 ) signal transduction pathway . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
SOCS 2 binds to the GH receptor and inhibits GH signaling , including attenuation of STAT 5 activation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Growth hormone ( GH ) insensitivity syndrome due to a GH receptor truncated after Box 1 , resulting in isolated failure of STAT 5 signal transduction . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
This could account for the inhibition of STAT 5 activation , because CIS competes with STAT 5 for GH receptor docking sites . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In liver , these responses were associated with increased abundance of the GH receptor protein ( GHR ; P < 0 . 05 ) , whereas the abundance of intracellular mediators of GH actions ( JAK 2 , STAT 5 , or STAT 3 ) remained unaffected . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH activated Janus associated kinase 2 ( JAK 2 ) signal transducers and activators of transcription 5 ( STAT 5 ) signaling was impaired in CRF rats , despite normal GH receptor ( GHR ) , JAK 2 , and STAT 5 protein levels . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
This was associated with down regulation of GH receptor ( GHR ) expression and impaired GH dependent STAT5b activation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
However , measurements of IGF 1 and its binding proteins , as well as GH and its binding proteins , can help us to focus our analysis of patients suspected to have genetic abnormalities on the GH receptor , IGF 1 , its receptor , IGFALS , or intracellular signalling proteins such as STAT5b or ERK . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH rapidly and potently stimulates IGF 1 gene transcription by mechanisms independent of new protein synthesis , and recent studies have linked the transcription factor Stat5b to a regulatory network connecting the activated GH receptor on the cell membrane to the IGF 1 gene in the nucleus . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Total GH receptor ( GHR ) , JAK 2 , and STAT 5 signaling proteins and the time course of STAT 5 activation were also measured in liver after recombinant human GH administration by immunoblot and electrophoretic mobility shift analysis . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Thus STAT5b and HNF4alpha exhibit bi directional cross talk that may augment HNF4alpha dependent gene transcription while inhibiting STAT5b transcriptional activity via the inhibitory effects of HNF4alpha on JAK 2 phosphorylation , which leads to inhibition of STAT5b signalling initiated by the GH receptor at the cell surface . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
At the tissue level , the action of GH is mediated by the GH receptor ( GHR ) and the receptor activated intracellular signaling pathway involving Janus kinase 2 ( JAK 2 ) and signal transducer and activator of transcription 5 ( STAT 5 ) . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In order to establish whether the prolactin activated transcription factor Stat 5 ( mammary gland factor ) is also activated by growth hormone , nuclear extracts were prepared from COS 7 cells transiently expressing transfected Stat 5 and growth hormone receptor cDNA . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Differential activation of Stat 3 and Stat 5 by distinct regions of the growth hormone receptor . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Identification of growth hormone receptor ( GHR ) tyrosine residues required for GHR phosphorylation and JAK 2 and STAT 5 activation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Activation of STAT 5 was also observed after growth hormone treatment of cells transfected with cDNA expression plasmids for several different truncated growth hormone receptor mutants , although this activation was less efficient than with the wild type receptor . ^^^ Point mutation of individual tyrosines in the growth hormone receptor intracellular domain to phenylalanines had no significant effect on signal transduction via STAT 5 . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The aim of this study was to investigate the interaction of Stat 5 with key effector proteins Erk 2 and Shc after activation by growth hormone ( GH ) , using Chinese Hamster Ovary ( CHO ) cells stably expressing the wild type rabbit growth hormone receptor ( GHR ) . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
A novel C terminal growth hormone receptor ( GHR ) mutation results in impaired GHR STAT 5 but normal STAT 3 signaling . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Growth hormone induced JAK 2 , growth hormone receptor ( GHR ) , and STAT 5 tyrosine phosphorylation was significantly depressed in CRF as was nuclear translocation of phosphorylated STAT 5 . ^^^ Growth hormone receptor , JAK 2 , STAT5a , and STAT5b protein levels were unaltered in CRF . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
GH dependent tyrosyl phosphorylation of Stat 5 , but not Stats 1 or 3 , was reduced in CHO cells expressing GHR 1 454 . ^^^ These findings suggest that : 1 ) JAK 2 is required for GH dependent phosphorylation of Stats 1 , 3 , and 5 ; 2 ) tyrosines 333 and / or 338 are required for maximal tyrosyl phosphorylation of Stats 1 , 3 , and 5 ; 3 ) Stat 5 binds to a phosphorylated tyrosine ( s ) within amino acids 454 638 in addition to tyrosines 333 and / or 338 ; 4 ) GH stimulates tyrosyl phosphorylation of JAK 1 in addition to JAK 2 with JAK 2 having a much greater response ; 5 ) some Stat 3 and Stat 5 ( and possibly Stat 1 ) may bind to nonphosphorylated amino acids in GHR or to phosphorylated tyrosines in proteins that bind to GHR ( e . g . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The present study investigates the importance of the GHR cytoplasmic domain in the activation of STAT 3 and STAT5b . ^^^ STAT5b and STAT 3 , when each was coexpressed in COS 7 cells with WT GHR and WT JAK 2 , were both strongly tyrosine phosphorylated in response to GH . ^^^ Coexpression of STAT 3 with GHR / JAK 2 chimeras resulted in a strong GH independent tyrosine phosphorylation of STAT 3 that was 40 % as active as that seen with WT GHR plus WT JAK 2 , whereas STAT5b was more minimally phosphorylated ( 13 % of WT GHR plus WT JAK 2 ) when coexpressed with chimeras devoid of the GHR cytoplasmic domain . ^^^ Transient coexpression of each STAT together with WT JAK 2 and GHR COOH terminal truncation mutants indicated that a GH induced STAT 3 DNA binding complex , but not a STAT5b DNA binding complex , was detectable when a GHR devoid of 85 % of the cytoplasmic domain COOH terminus ( but eliciting significant JAK 2 tyrosine phosphorylation ) was expressed . ^^^ We conclude that GH induces activation of STAT 3 and STAT5b by two different pathways : one primarily dependent on activation of JAK 2 ( STAT 3 ) and another that is additionally reliant on the presence of an intact and tyrosine phosphorylated GHR cytoplasmic domain ( STAT5b ) . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Mutant GH was less potent than wild type GH not only in phosphorylation of tyrosine residues in GHR , janus kinase 2 ( JAK 2 ) , and signal transducers and activators of transcription 5 ( STAT 5 ) in IM 9 cells , but also in metabolic responses of BaF / GM cells , a stable clone transfected with cDNA of the chimera of the extracellular domain of human GHR , the transmembrane and the cytoplasmic domain of the human thrombopoietin receptor . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Mutant human receptor protein was expressed at a lower level than wild type receptor and its activity was reduced : GH dependent signal transducer and activator of transcription 5 ( Stat 5 ) mediated transactivation of a reporter gene was lower in 293 cells transfected with mutant GHR cDNA than in transfected cells expressing a comparable level of wild type GHR . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Moreover , in GH induced tyrosine phosphorylation of signal transducer and activator of transcription 5 ( STAT 5 ) , GHR 277 exerted a dominant negative effect when GHR 277 and GHR fl were cotransfected . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In summary , continuous insulin treatment in rat H 4 hepatoma cells reduces GH binding , immunoreactive GHR , GH induced phosphorylation of JAK 2 , and GH induced tyrosine phosphorylation of STAT5B . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
By using both transient and stable expression systems , we determined that cysteine 241 ( an unpaired extracellular cysteine ) was critical for GH induced GHR disulfide linkage ; however , GH induced GHR dimerization , GHR JAK 2 interaction , and GHR , JAK 2 , and STAT 5 tyrosine phosphorylation still proceeded when this cysteine residue was mutated . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Moreover , STAT 5 mediated transcriptional activation of GHR C422F expressing cells was comparable to that of GHR wt expressing cells . ^^^ These findings indicated that the C422F mutation of GHR affected neither GH induced tyrosine phosphorylation nor the transcriptional activation of STAT 5 . ^^^ There were no significant differences in GH induced tyrosine phosphorylation of STAT5b ( signal transducer and activator of transcription ) between wild type GHR ( GHR wt ) and mutant GHR ( GHR C422F ) expressing cells . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Mutation of GHR ' s membrane proximal tyrosine residues 333 and 338 to phenylalanine suppressed the inhibition by SOCS 3 , but not by CIS , of GH signaling to STAT5b . ^^^ SOCS / CIS proteins can thus inhibit GH signaling to STAT5b by three distinct mechanisms , distinguished by their molecular targets within the GHR JAK 2 signaling complex , as exemplified by SOCS 1 ( direct JAK 2 kinase inhibition ) , SOCS 3 ( inhibition of JAK 2 signaling via membrane proximal GHR tyrosines 333 and 338 ) , and CIS and SOCS 2 ( inhibition via membrane distal tyrosine ( s ) ) . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
However , GH was unable to cause transactivation of reporter gene constructs harboring Stat 5 binding sites ( the GHREII from the rat spi 2 . 1 gene promoter , and the LHRE from the rat beta casein gene promoter ) , except in cells transiently transfected with either Stat 5 cDNAs or the rat GHR cDNA . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Whereas bone length was reduced in Stat5ab ( / ) mice , there was no reduction in trabecular bone remodeling or growth plate width as observed in GHR ( / ) mice , indicating that the effects of GH in bone may not involve Stat 5 activation . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Recovery of GH induced STAT 5 phosphorylation correlated with the time dependent recovery of GHR levels during incubation in the absence of GH . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The signal transducer and activator of transcription 5 ( STAT 5 ) mediated transcriptional activation in mutant GHR ( GHR T51I ) expressing cells was significantly reduced as compared with wild type GHR ( GHR wt ) expressing cells . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Upon GH stimulation of the GHR positive CMT U 335 canine mammary tumor cell line , the transcription factors STAT5A and STAT5B become phosphorylated on their tyrosine residues , which is likely to reflect the significance of mammary GH in vivo . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In C2C12 ( GHR ) cells , we observed an increased activation of the Janus kinase 2 ( Jak 2 ) , signal transducers and activator of transcription 5 ( Stat 5 ) , mitogen activated protein kinase ( MAPK ) and protein kinase B ( Akt ) upon acute GH stimulation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The effect of heterodimerization was studied by comparing activation of Janus kinase 2 , signal transducer and activator of transcription ( STAT ) 1 , STAT 3 , STAT 5 , and MAPK in Chinese hamster ovary cells stably transfected with chimeric genes encoding receptors consisting of cytosolic and transmembrane parts of oGHR and oPRLR , extracellular domains of human granulocyte and macrophage colony stimulating factor ( hGM CSF ) receptor alpha or beta , and cells transfected with the two forms ( alpha or beta ) of PRLR and GHR . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
We found decreased hepatic and renal expression of CYP2C11 in ethanol fed rats with concomitant decreases in STAT5b and phospho STAT5b , decreased in vitro hepatic STAT5b binding to a CYP2C11 promoter element and no effects on hepatic GHR levels . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Since GH regulates cellular cytoskeleton with potential implications in GH signaling , we investigated the effects of actin cytoskeleton disruption on the kinetics of GH activated GHR / Janus kinase 2 ( JAK 2 ) / signal transducer and activator of transcription 5 ( STAT 5 ) signaling pathway . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Correspondingly , GH induced tyrosine phosphorylated GHR , JAK 2 , and ERK1 / 2 were highly represented in the CM fraction , whereas tyrosine phosphorylated STAT 5 was enriched in the non membranous fraction of the post nuclear supernatant . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
In the cell cultures , STAT 5 ( signal transducer and activator of transcription 5 ) phosphorylation was measured by Western blot analysis as an index of GHR signalling ; cell proliferation was evaluated by [ H 3 ] thymidine incorporation and glycoprotein hormone production analysed by radioimmunoassay ( RIA ) . ^^^ RESULTS : All adenomas investigated expressed the GHR , but there was no detection of STAT 5 phosphorylation . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
We hypothesized that reduced IGF 1 expression in obstructive cholestasis would be associated with downregulation of the GHR and impaired phosphorylation of signal transducers and activators of transcription ( STAT 5 ) . ^^^ GHR , STAT 5 , Sp 3 , and IGF 1 expression and / or DNA binding were assessed using immunoblots , electrophoretic mobility shift assays , and / or real time RT PCR . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Incubation of cells with IL 1beta ( 10 ng / ml , 24 h ) had no effect on the relative abundance of GHR or signaling proteins JAK 2 , STAT5b , and ERK1 / 2 in cell lysates . ^^^ Baseline phosphorylation of GHR , JAK 2 , STAT5b , and ERK1 / 2 was minimal . ^^^ After GH stimulation , tyrosine phosphorylation of GHR , JAK 2 , STAT5b , and ERK1 / 2 increased 2 to 10 fold . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
The cytokine inducible SH 2 domain containing protein CIS inhibits signaling from the growth hormone ( GH ) receptor ( GHR ) to STAT5b by a proteasome dependent mechanism . ^^^ GHR signaling to STAT5b continued for approximately 30 40 min , however , indicating that GHR signaling and deactivation of the GH . ^^^ The internalization of GH and GHR was inhibited by CIS R107K , a dominant negative SH 2 domain mutant of CIS , and by the proteasome inhibitors MG 132 and epoxomicin , which prolong GHR signaling to STAT5b . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Western blot analysis showed GH induced phosphorylation of Jak 2 and Stat 5 for both GHR 1 656 and GHRwt , although reduced Stat 5 activity was detected with GHR 1 656 , consistent with lower levels of expression of GHR 1 656 than GHRwt at the cell surface . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
To that end , we compared : ( a ) the expression of GHR and PRLR mRNAs in 3T3 L 1 preadipocytes during the course of adipocyte differentiation ; ( b ) the induction of STAT 5 activity by GH and PRL during adipogenesis ; and ( c ) the acute effects of GH and PRL on the suppressors of cytokine signaling ( SOCS 1 3 and cytokine inducible SH 2 domain containing protein CIS ) and IGF 1 . ^^^ Our findings reveal disparities in the expression of PRLR and GHR during adipocyte development and differential effects of the hormones on STAT 5 , the SOCS proteins , CIS , and IGF 1 . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
Recently , two female patients with severe growth retardation and pulmonary and immunological problems were described with a defect in STAT5b , a critical intermediary of downstream GHR signaling . ^^^ Therefore , in patients with severe short stature , signs of GH insensitivity , and a normal GHR , analysis of the STAT5b gene is recommended . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
To study GHR dimerization or GH induced conformational change of predimerized GHRs and STAT 5 activation kinetics in intact cells , fluorescence resonance energy transfer ( FRET ) and live cell imaging methods were employed . ^^^ FRET measurements at the membrane of HEK 293T cells co expressing GHRs tagged at the C terminus with cyan ( C ) and yellow ( Y ) fluorescent proteins ( FPs ) revealed transient GHR dimerization lasting 2 3 min , with a maximum at 3 min after GH stimulation , which was sufficient to induce STAT 5 activation . ^^^ YFP tagged STAT 5 recruitment to the membrane , binding to GHR CFP , and phosphorylation , occurred within minutes of GH stimulation . ^^^ Although GHR dimerization and STAT 5 activation have been reported previously , this is the first spatially resolved demonstration of GHR signaling kinetics in intact cells . . ^^^
Interacting proteins: P10912 and P51692 Pubmed SVM Score :0.0
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