Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.82842481
Similar to TNF , cycloheximide and anisomycin stimulated serine phosphorylation of IRS 1 and IRS 2 , reduced their ability to interact with the insulin receptor , inhibited the insulin induced tyrosine phosphorylation of IRS proteins , and diminished their association with phosphatidylinositol 3 kinase ( PI3K ) . 0.82842481^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.6378474
Insulin receptor substrate 2 binds to the insulin receptor through its phosphotyrosine binding domain and through a newly identified domain comprising amino acids 591 786 . 0.6378474^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The type 1 interferon receptor mediates tyrosine phosphorylation of insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Pivotal role for insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
However , substitution of the Pro 10 Pro in the BOX 1 motif with Ala 10 Ala failed to abolish IL 9 induced cell proliferation but decreased IL 9 mediated tyrosine phosphorylation of JAK kinases , insulin receptor substrate 2 , and signal transducer and activator of transcription 3 ( STAT 3 ) and expression of c myc and junB . ^^^ Deletion of this region significantly impaired IL 9 induced cell growth , activation of JAK kinases , insulin receptor substrate 2 , and STAT 3 and expression of early response genes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Erythropoietin induces the tyrosine phosphorylation of insulin receptor substrate 2 . ^^^ In this report , we demonstrate that insulin receptor substrate 2 ( IRS 2 ) is phosphorylated on tyrosine following treatment of UT 7 cells with erythropoietin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Interaction of insulin receptor substrate 2 ( IRS 2 ) with the insulin and insulin like growth factor 1 receptors . ^^^ Insulin receptor substrate 2 ( IRS 2 ) has recently been shown to be a substrate of the insulin receptor ( IR ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin resistance and growth retardation in mice lacking insulin receptor substrate 1 and identification of insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Growth hormone , interferon gamma , and leukemia inhibitory factor utilize insulin receptor substrate 2 in intracellular signaling . ^^^ In this report , we demonstrate that insulin receptor substrate 2 ( IRS 2 ) is tyrosyl phosphorylated following stimulation of 3T3 F442A fibroblasts with growth hormone ( GH ) , leukemia inhibitory factor and interferon gamma . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 is the major 170 kDa protein phosphorylated on tyrosine in response to cytokines in murine lymphohemopoietic cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A leptin mediated recruitment of phosphatidylinositol 3 kinase to insulin receptor substrate 2 was also detected . ^^^ However , no significant tyrosine phosphorylation of insulin receptor substrate 2 and modulation of the immediate cell response to insulin were observed . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Tyr 624 and Tyr 628 in insulin receptor substrate 2 mediate its association with the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 ( IRS 2 ) can mediate the action of insulin to stimulate translocation of GLUT 4 to the cell surface in rat adipose cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Leptin activates PI 3 kinase in C2C12 myotubes via janus kinase 2 ( JAK 2 ) and insulin receptor substrate 2 ( IRS 2 ) dependent pathways . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Both INS and IGF 1 activated signal transduction in maturing human erythropoietic cells as determined by phosphorylation of the insulin receptor substrate 2 ( IRS 2 ) protein . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A role for the insulin interleukin ( IL ) 4 receptor motif of the IL 4 receptor alpha chain in regulating activation of the insulin receptor substrate 2 and signal transducer and activator of transcription 6 pathways . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin like growth factor 1 induced DNA synthesis in insulin secreting cell line RINm5F is associated with phosphorylation of the insulin like growth factor 1 receptor and the insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 amino acid polymorphisms are not associated with random type 2 diabetes among Caucasians . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A specific increased expression of insulin receptor substrate 2 in pancreatic beta cell lines is involved in mediating serum stimulated beta cell growth . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 a new candidate gene for NIDDM . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Enhanced expression of the insulin receptor substrate 2 docking protein in human pancreatic cancer . ^^^ Insulin receptor substrate 2 ( IRS 2 ) is a multisite docking protein implicated in mitogenic signaling after activation of the insulin and insulin like growth factor ( IGF ) 1 receptors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential regulation of insulin receptor substrate 2 and mitogen activated protein kinase tyrosine phosphorylation by phosphatidylinositol 3 kinase inhibitors in SH SY5Y human neuroblastoma cells . ^^^ We report here that IGF 1 stimulated the tyrosine phosphorylation of the type 1 IGF receptor ( IGF IR ) and insulin receptor substrate 2 ( IRS 2 ) in a time and concentration dependent manner . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Mapping of the human insulin receptor substrate 2 gene , identification of a linked polymorphic marker and linkage analysis in families with Type 2 diabetes : no evidence for a major susceptibility role . ^^^ Insulin receptor substrate 2 ( IRS 2 ) is a substrate of the insulin receptor and mediates the action of the insulin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 and Shc play different roles in insulin like growth factor 1 signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Human insulin receptor substrate 2 ( IRS 2 ) is a primary progesterone response gene . ^^^ In our search for cellular target genes induced through this cross talk mechanism , we identified human insulin receptor substrate 2 ( IRS 2 ) , a cytoplasmic signaling molecule that mediates effects of insulin , insulin like growth factor 1 ( IGF 1 ) , and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Exclusion of insulin receptor substrate 2 ( IRS 2 ) as a major locus for early onset autosomal dominant type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IL 4 ( not IL 3 or GM CSF ) induced tyrosine phosphorylation of insulin receptor substrate 2 ( IRS 2 ) and its association with phosphatidylinositol 3 kinase ( PI 3 K ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Mutation of tyrosine 960 within the insulin receptor juxtamembrane domain impairs glucose transport but does not inhibit ligand mediated phosphorylation of insulin receptor substrate 2 in 3T3 L 1 adipocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 is not necessary for insulin and exercise stimulated glucose transport in skeletal muscle . ^^^ Insulin receptor substrate 2 deficient ( IRS 2 ( / ) ) mice develop type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
TNF alpha suppresses IGF 1 induced tyrosine phosphorylation of insulin receptor substrate 2 ( IRS 2 ) and inhibits IRS 2 precipitable phosphatidylinositol 3 ' kinase activity . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Human insulin receptor substrate 2 : gene organization and promoter characterization . ^^^ Insulin receptor substrate 2 ( IRS 2 ) belongs to a family of cytoplasmic adaptor proteins , which link insulin , IGF 1 , and cytokine receptor tyrosine kinases to signaling pathways regulating metabolism , growth , and differentiation ( 1 3 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Search for variants of the gene promoter and the potential phosphotyrosine encoding sequence of the insulin receptor substrate 2 gene : evaluation of their relation with alterations in insulin secretion and insulin sensitivity . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Developmental expression of insulin receptor substrate 2 during dimethylsulfoxide induced differentiation of human HL 60 cells . ^^^ Insulin receptor substrate 2 ( IRS 2 ) is phosphorylated on tyrosine by a number of cytokine receptors and is implicated in the activation of phosphatidylinositol 3 ' kinase ( PI 3 kinase ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This pathway involves stimulation of phosphatidylinositol 3 kinase ( PI3K ) binding to insulin receptor substrate 1 and insulin receptor substrate 2 , activation of PI3K and protein kinase B ( AKT ) , and PI3K dependent activation of cyclic nucleotide phosphodiesterase 3B , a cAMP degrading enzyme . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 , 3 , and 4 were not induced by estradiol . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Essential role of insulin receptor substrate 2 in insulin stimulation of Glut 4 translocation and glucose uptake in brown adipocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin receptor substrate 2 / phosphatidylinositol 3 ' kinase / p70S6K pathway was found to be either constitutively or IGF 1 dependently activated in all plasma cell tumors . ^^^ Taken together , these results demonstrate that up regulation and activation of IGF IR and the downstream signaling pathway involving insulin receptor substrate 2 , phosphatidylinositol 3 ' kinase , and p70S6K may play an important role in the development of a broad spectrum of plasma cell tumors . . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Studying intracellular signaling pathways , which may be involved in malignant transformation of Ret 9bp expressing NIH3T3 cells , we could demonstrate Ret 9bp dependent phosphorylation of insulin receptor substrate 2 ( IRS 2 ) with consecutive activation of phosphatidylinositol 3 kinase ( PI 3 kinase ) and protein kinase B ( PKB / AKT ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Here we show that deletion of insulin receptor substrate 2 ( IRS 2 ) , a component of the insulin / insulin like growth factor 1 signalling cascade , causes female infertility . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Role of insulin receptor substrate 2 in interleukin 9 dependent proliferation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin receptor substrate 2 ( IRS 2 ) is a major insulin signalling molecule . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
It was found that IL 4 induced phosphorylation of Janus kinases 1 and 3 , IL 4R alpha , signal transducer and activator of transcription 6 , and insulin receptor substrate 2 was strikingly but transiently inhibited by TCR ligation both in conventional and TCR transgenic T cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Disruption of insulin receptor substrate 2 causes type 2 diabetes because of liver insulin resistance and lack of compensatory beta cell hyperplasia . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
PTEN expression causes feedback upregulation of insulin receptor substrate 2 . ^^^ The gene encoding insulin receptor substrate 2 ( IRS 2 ) , a mediator of insulin signaling , was found to be the most induced gene at all time points . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Furthermore , IL 4 driven cell expansion was also normal in the cells infected with Y497F , although cells infected with Y497F were not capable of phosphorylating insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In summary , overexpression of IGF 1 receptors in Ir / hepatocytes normalized insulin receptor substrate 2 phosphorylation and glycogen synthesis to wild type levels , whereas it increased cell proliferation above wild type levels . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 phosphorylation is necessary for protein kinase C zeta activation by insulin in L6hIR cells . ^^^ We have investigated glycogen synthase ( GS ) activation in L6hIR cells expressing a peptide corresponding to the kinase regulatory loop binding domain of insulin receptor substrate 2 ( IRS 2 ) ( KRLB ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
E 2 induced degradation of uterine insulin receptor substrate 2 : requirement for an IGF 1 stimulated , proteasome dependent pathway . ^^^ For instance , uterine insulin receptor substrate 2 was decreased markedly at 24 h after E 2 treatment of mice . ^^^ In the present study we used various in vivo experimental approaches to examine the mechanism by which E 2 influences uterine insulin receptor substrate 2 expression . ^^^ Uterine insulin receptor substrate 2 mRNA levels were diminished after E 2 treatment , but this diminution did not account for the total reduction in insulin receptor substrate 2 protein , suggesting that the E 2 induced decrease in insulin receptor substrate 2 is not regulated solely at the mRNA level . ^^^ Cotreatment with progesterone prevented the E 2 stimulated reduction in insulin receptor substrate 2 protein at 24 h after hormone exposure . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IL 10 inhibits apoptosis of promyeloid cells by activating insulin receptor substrate 2 and phosphatidylinositol 3 ' kinase . ^^^ IL 10 increased tyrosyl phosphorylation of insulin receptor substrate 2 and stimulated the enzymatic activity of both phosphatidylinositol 3 ' kinase and Akt . ^^^ This survival promoting activity is totally due to IL 10 stimulating the insulin receptor substrate 2 / PI 3 kinase / Akt pathway , which increases the anti apoptotic activity of ERK1 / 2 . . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The prevalent Gly1057Asp polymorphism in the insulin receptor substrate 2 gene is not associated with impaired insulin secretion . ^^^ Disruption of the insulin receptor substrate 2 was shown to cause type 2 diabetes in mice . ^^^ In humans , a prevalent polymorphism in insulin receptor substrate 2 ( Gly1057Asp ) was not found be associated with type 2 diabetes in linkage and association studies . ^^^ In conclusion , our findings strongly suggest that the Gly1057Asp polymorphism in insulin receptor substrate 2 is not associated with beta cell dysfunction . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin is an important regulator of growth and initiates its action by binding to its receptor , which undergoes tyrosyl autophosphorylation and further enhances its tyrosine kinase activity towards other intermediate molecules , including insulin receptor substrate 1 , insulin receptor substrate 2 , and Shc . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
High fructose diet preserves beta cell mass and prevents diabetes in nonobese diabetic mice : A potential role for increased insulin receptor substrate 2 expression . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Regulation of breast cancer cell motility by insulin receptor substrate 2 ( IRS 2 ) in metastatic variants of human breast cancer cell lines . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Enhanced expression of insulin receptor substrate 2 and activation of protein kinase B / Akt in regenerating pancreatic duct epithelium of 60 % partial pancreatectomy rats . ^^^ We investigated the expression and roles of insulin receptor substrate 2 ( IRS 2 ) and protein kinase B / Akt ( Akt ) in pancreatic regeneration that starts with the common duct epithelium using a non diabetic model of beta cell adaptation and mass expansion , 60 % pancreatectomy rats . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In addition , insulin dependent tyrosine phosphorylation of insulin receptor substrate 2 was enhanced in the p85beta ( / ) mice , a phenotype not observed in the p85alpha ( / ) mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Activation of PI3K by glucose dependent insulinotropic polypeptide and glucose was associated with insulin receptor substrate isoforms insulin receptor substrate 2 and growth factor bound 2 associated binder 1 and PI3K isoforms p85alpha , p110alpha , p110beta , and p110gamma . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Increased expression of the sterol regulatory element binding protein 1 gene in insulin receptor substrate 2 ( / ) mouse liver . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Regulation of insulin / insulin like growth factor 1 signaling by proteasome mediated degradation of insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Overexpression of insulin receptor substrate 1 , but not insulin receptor substrate 2 , protects a T cell hybridoma from activation induced cell death . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Short term leptin dependent inhibition of hepatic gluconeogenesis is mediated by insulin receptor substrate 2 . ^^^ Physiological leptin concentrations ( 0 . 1 5 nmol / liter ) stimulated the tyrosine phosphorylation ( pY ) of insulin receptor substrate 2 ( IRS 2 ) ( 280 954 % ; P < 0 . 05 ) and its associated phosphatidylinositol 3 kinase activity ( 122 621 % ; P < 0 . 003 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In addition , an increase in insulin receptor substrate 2 protein and a differential regulation of the phosphatidylinositol 3 kinase regulatory subunit ( p85alpha ) isoforms expression were found in fat from antisense treated animals , although increased insulin sensitivity measured by protein kinase B phosphorylation was not observed . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To determine whether integrin function could interfere with IGF signaling , we used an alpha 5 beta 1 blocking peptide to show that in MDA 231BO cells integrin occupancy appeared necessary for phosphorylation of insulin receptor substrate 2 but not for IGFR 1 activation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin signaling proteins , insulin receptor substrate 2 and phosphatidylinositol 3 ( PI 3 ) kinase regulatory subunit p50alpha , were increased and PI 3 kinase p85alpha expression was decreased in liver and fat . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The C / EBPbeta / delta deficient adipocytes also expressed lower amounts of insulin receptor substrate 2 ( IRS 2 ) than the adipocytes derived from wild type MEFs , whereas the amounts of insulin receptor and IRS 1 were similar . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Identification of a single nucleotide polymorphism showing no insulin mediated suppression of the promoter activity in the human insulin receptor substrate 2 gene . ^^^ AIMS / HYPOTHESIS : To understand the transcriptional regulation and to investigate the pathological influence upon Type 2 ( non insulin dependent ) diabetes mellitus of insulin receptor substrate 2 ( IRS 2 ) , the 5 ' flanking region of the human IRS 2 gene was cloned and screened in Japanese diabetic patients . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Immunoprecipitation Western blotting experiments showed that phosphorylation levels of IL 4Ralpha , Janus kinase 1 , insulin receptor substrate 1 , and insulin receptor substrate 2 , factors with molecular mass close to the observed 130 kDa major phosphorylation band , increased in response to the combined CD 40 ligation and IL 4 action . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Similarly , mice with loss of brain insulin receptors or insulin receptor substrate 2 deficiency exhibit neuroendocrine defects and female infertility . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Whereas insulin receptor autophosphorylation and insulin receptor substrate 2 tyrosine phosphorylation were not affected by PUGNAc inhibition of O GlcNAcase , downstream phosphorylation of Akt at Thr 308 and glycogen synthase kinase 3 beta at Ser 9 was inhibited . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We show that phosphoinositide 3 ( PI 3 ) kinase associates with 4 tyrosine phosphorylated proteins in primary human erythroid progenitors , namely insulin receptor substrate 2 ( IRS 2 ) , Src homology 2 domain containing inositol 5 ' phosphatase ( SHIP ) , Grb 2 associated binder 1 ( Gab 1 ) , and the Epo receptor ( EpoR ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Polymorphisms of the insulin receptor substrate 2 in patients with type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Metformin rapidly increases insulin receptor activation in human liver and signals preferentially through insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
By immunohistochemistry , most insulin receptor ( IR ) and insulin receptor substrate 2 ( IRS 2 ) immunoreactivity localized to the arcuate nucleus . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In order to understand the role of the insulin receptor substrate 2 ( IRS 2 ) gene ( chromosome region : 13q34 ) in obesity , a complex disorder associated with insulin resistance and glucose intolerance , we determined single nucleotide polymorphims ( SNPs ) and complex haplotypes in women with morbid obesity and a body mass index ( BMI ) of 41+ / 0 . 8 kg / m2 ( n=99 ) compared with controls having a BMI of 23 . 8+ / 0 . 1 kg / m2 ( n=92 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin release was measured by radioimmuoassay , the expression level of insulin receptor substrate 2 ( IRS 2 ) protein and the association of IRS 2 with p 85 subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) were determined by immunoprecipitation and Western blot . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Lack of insulin receptor substrate 2 causes progressive neointima formation in response to vessel injury . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
TGase 2 / mice manifest a tendency to develop hypoglycemia after administration of exogenous insulin as a consequence of enhanced insulin receptor substrate 2 ( IRS 2 ) phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 maintains predominance of anabolic function over catabolic function of osteoblasts . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Mice lacking insulin receptor substrate 2 ( Irs 2 ) develop beta cell failure , suggesting that insulin signaling is required to maintain an adequate beta cell mass . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Regulation of insulin receptor substrate 2 tyrosine phosphorylation in animal models of insulin resistance . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 deficiency impairs brain growth and promotes tau phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A high concentration of insulin decreased the activity of phosphofructo 1 kinase ( PFK ) , pyruvate dehydrogenase ( PDH ) and glycogen synthase ( GS ) , as well as decreasing the expression of insulin receptor ( IR ) and insulin receptor substrate 2 ( IRS 2 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A novel insulin analog with unique properties : LysB 3 , GluB 29 insulin induces prominent activation of insulin receptor substrate 2 , but marginal phosphorylation of insulin receptor substrate 1 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Furthermore , a reduction of p 85 beta specifically increases insulin receptor substrate 2 phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Progesterone crosstalks with insulin like growth factor signaling in breast cancer cells via induction of insulin receptor substrate 2 . ^^^ We found that insulin receptor substrate 2 ( IRS 2 ) levels were markedly induced by progesterone and the synthetic progestin R 5020 in MCF 7 and other progesterone receptor ( PR ) positive breast cancer cell lines , whereas IRS 1 and the IGF 1 receptor were not induced . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This effect correlated with the potentiation by rosiglitazone of insulin stimulated Tyr phosphorylation of insulin receptor substrate 1 and to a greater extent of insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin down regulates insulin receptor substrate 2 expression through the phosphatidylinositol 3 kinase / Akt pathway . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Upregulation of insulin receptor substrate 2 in pancreatic beta cells prevents diabetes . ^^^ The insulin receptor substrate 2 ( Irs 2 ) branch of the insulin / IGF signaling system coordinates peripheral insulin action and pancreatic beta cell function , so mice lacking Irs 2 display similarities to humans with type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Detection of single nucleotide polymorphisms in insulin receptor substrate 2 gene 3 ' untranslated region by denaturing high performance liquid chromatography ] . ^^^ OBJECTIVE : To explore the use of denaturing high performance liquid chromatography ( DHPLC ) in detecting single nucleotide polymorphisms ( SNPs ) of insulin receptor substrate 2 ( IRS 2 ) gene 3 ' untranslated region ( 3 ' UTR ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Variation of insulin receptor substrate 2 gene 3 ' untranslated region in patients with type 2 diabetes mellitus ] . ^^^ Insulin receptor substrate 2 ( IRS 2 ) belongs to a family of cytoplasmic adaptor proteins , which link insulin , insulin like growth factor 1 ( IGF 1 ) , and cytokine receptor tyrosine kinases to signaling pathways regulating metabolism , growth , differentiation , reproduction , and homestasis . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Leptin treatment at this dose improved insulin stimulated insulin receptor and insulin receptor substrate 2 ( IRS 2 ) phosphorylation , IRS 2 associated PI3K activity , and Akt activity in liver . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Transgenic expression of insulin receptor substrate 2 in murine B cells alters the cell density dependence of IgE production in vitro and enhances IgE production in vivo . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Recent data on experimental atherosclerosis in mice show that ( a ) insulin administration reduces the number and the size of atherosclerotic lesions in apolipoprotein E null mice ; and ( b ) in insulin receptor substrate 2 null mice , the interruption in insulin signal transduction results in enhanced atherogenicity . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Surprisingly , the expression of glucose transporters 1 and 4 and insulin receptor substrate 2 was increased and that of pyruvate dehydrogenase kinase 4 and insulin receptor substrate 1 was reduced . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 ( IRS 2 ) is the main mediator of insulin signalling in the liver , controlling insulin sensitivity . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Another signal on chromosome 13 was observed at D13S285 ( LOD = 1 . 86 ) , where the insulin receptor substrate 2 gene resides . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The aim of our study was to investigate whether common polymorphisms in the genes regulating the early insulin signalling pathway ( insulin ; A 23T , insulin like growth factor 1 receptor [ IGF 1R ] ; GAG1013GAA , plasma cell membrane glycoprotein 1 [ PC 1 ] ; K121Q , insulin receptor substrate [ IRS 1 ] ; G972R , insulin receptor substrate 2 [ IRS 2 ] ; G1057D and phosphatidylinositol 3 kinase p 85 alpha [ PI3K ] ; M326I ) affect the weight change and development of Type 2 diabetes in the Finnish Diabetes Prevention Study . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Instead , IL 1beta inhibited the ability of IGF 1 to phosphorylate tyrosine residues on both of its downstream docking proteins , insulin receptor substrate 1 and insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Deletion of the pleckstrin and phosphotyrosine binding domains of insulin receptor substrate 2 does not impair its ability to regulate cell proliferation in myeloid cells . 32D IGF 1 receptor ( IR ) cells are IL 3 dependent myeloid cells that can be induced to differentiate into granulocytes by IGF 1 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin signal was transduced through the insulin receptor substrate 2 ( IRS 2 ) pathway and the activity of IRS 2 was decreased gradually at the messenger ribonucleic acid ( mRNA ) level during cold preservation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulated insulin receptor substrate 2 and Akt 2 phosphorylation were significantly blunted in IUGR rats . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Dysregulation of insulin receptor substrate 2 in beta cells and brain causes obesity and diabetes . ^^^ Here we show that a conditional knockout of insulin receptor substrate 2 ( Irs 2 ) in mouse pancreas beta cells and parts of the brain including the hypothalamus increased appetite , lean and fat body mass , linear growth , and insulin resistance that progressed to diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The recent identification of insulin receptor substrate 2 ( IRS 2 ) as a central player in the pathophysiology of many of these processes suggests a potentially unifying molecular link underlying the initiation and progression of type 2 diabetes . . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 plays a crucial role in beta cells and the hypothalamus . ^^^ We previously demonstrated that insulin receptor substrate 2 ( Irs 2 ) KO mice develop diabetes associated with hepatic insulin resistance , lack of compensatory beta cell hyperplasia , and leptin resistance . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Involvement of insulin receptor substrate 2 in mammary tumor metastasis . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 ( IRS 2 ) deficient mice exhibit obesity associated with hypertrophic adipocytes and leptin resistance . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Furthermore , three other insulin controlled genes , namely IGFBP 1 ( insulin like growth factor binding protein 1 ) , IRS 2 ( insulin receptor substrate 2 ) and glucokinase , were regulated abnormally by feeding in the liver of PDK 1 deficient mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In addition , insulin receptor substrate 2 ( IRS 2 ) protein levels were reduced in IUGR placentas , with no changes in IRS 1 or Shc protein content , and this was associated with a parallel decrease in IRS 2 associated phosphatidyl inositol 3 kinase . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Both cell lines exhibit androgen responsive patterns of IGF 1 receptor ( IGF IR ) expression , activation , and signaling to insulin receptor substrate 2 and AKT . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Interestingly , a key signaling molecule that promotes beta cell growth and survival , insulin receptor substrate 2 ( IRS 2 ) , is a member of a family of proteins whose inhibition contributes to the development of insulin resistance in the liver and other insulin responsive tissues . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Here we show that p 27 ( Kip 1 ) progressively accumulates in the nucleus of pancreatic beta cells in mice that lack either insulin receptor substrate 2 ( Irs 2 ( / ) ) or the long form of the leptin receptor ( Lepr ( / ) or db / db ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 is essential for maturation and survival of photoreceptor cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 plays diverse cell specific roles in the regulation of glucose transport . ^^^ The insulin receptor substrate 2 ( IRS 2 ) protein is one of the major insulin signaling substrates . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
A commonly occurring nucleotide polymorphism of the insulin receptor substrate 2 ( IRS 2 ) gene at amino acid 1057 from Glycine to Asparaginic acid ( G1057D ) was recently shown to be a determinant of insulin sensitivity in both glucose tolerant individuals and those with type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Tyrosine phosphorylation and protein expression of insulin receptor substrate 2 in the adipose tissue from patients with polycystic ovary syndrome ] . ^^^ OBJECTIVE : To explore molecular mechanisms of insulin resistance of polycystic ovary syndrome ( PCOS ) by determining the tyrosine phosphorylation and protein expression of insulin receptor substrate 2 ( IRS 2 ) in adipose tissue from patients with PCOS . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The role of insulin receptor substrate 2 in hypothalamic and beta cell function . ^^^ Insulin receptor substrate 2 ( Irs 2 ) plays complex roles in energy homeostasis . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 proteasomal degradation mediated by a mammalian target of rapamycin ( mTOR ) induced negative feedback down regulates protein kinase B mediated signaling pathway in beta cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Attenuation of accumulation of neointimal lipid by pioglitazone in mice genetically deficient in insulin receptor substrate 2 and apolipoprotein E . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Socs 1 / Ifng / mice had elevated liver insulin receptor substrate 2 expression ( IRS 2 ) and IRS 2 tyrosine phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Four of these eighty five genes mapped near ARC QTL containing regions , including insulin receptor substrate 2 ( Irs 2 ) and acyl CoA synthetase long chain family member 1 ( Acsl 1 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Polymorphisms in the insulin receptor substrate 1 ( IRS 1 ) gene and the insulin receptor substrate 2 ( IRS 2 ) gene influence glucose homeostasis and body mass index in women with polycystic ovary syndrome and non hyperandrogenic controls . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Estrogen and exercise may enhance beta cell function and mass via insulin receptor substrate 2 induction in ovariectomized diabetic rats . ^^^ Increased beta cell proliferation was associated with the induction of insulin receptor substrate 2 and pancreatic homeodomain protein 1 via the activation of the cAMP response element binding protein . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Overexpression of insulin receptor substrate 2 in human and murine hepatocellular carcinoma . ^^^ Although intracellular insulin receptor substrate 2 ( IRS 2 ) is the main effector of insulin signaling in the liver , its role in hepatocarcinogenesis is unknown . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This study showed that tramadol activated a neuronal insulin signaling cascade by increasing the induction of insulin receptor substrate 2 expression in primary cultured neuronal cells while this activation was suppressed by naloxone ( MOR inhibitor ) and dexamethasone ( non specific inhibitor of MOR and 5 HT receptor , DEX ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin like growth factor 1 induces preferential degradation of insulin receptor substrate 2 through the phosphatidylinositol 3 kinase pathway in human neuroblastoma cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In addition , HL feeding leads to impaired functional and molecular activation of the insulin signaling pathway , which is paralleled by increased serine phosphorylation of the insulin receptor and insulin receptor substrate 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Although Chromosome 8 has not been previously associated with imprinting effects , features of candidate genes within the QTL confidence interval ( CpG islands and direct clustered repeats ) support the hypothesis that Insulin receptor substrate 2 may be associated with imprinting , but as yet is unidentified as being so . . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin receptor substrate 2 ( Irs 2 ) branch of the insulin / insulin like growth factor signaling cascade prevents diabetes in mice because it promotes beta cell replication , function , and survival , especially during metabolic stress . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
However , such renal protective effects of ghrelin on ischemia / reperfusion injury were not observed in insulin receptor substrate 2 knockout mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
With respect to these causal factors , we focus on Fas , the ATP sensitive K+ channel , insulin receptor substrate 2 , oxidative stress , nuclear factor kappaB , endoplasmic reticulum stress , and mitochondrial dysfunction as their respective mechanisms of action . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IGF 1 receptor and insulin receptor substrate 2 levels were increased in LTED versus the Wt MCF 7 cells , and ICI but not TAM reduced their expression in a dose dependent fashion . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In a functional genetic screen of HD , we found that activation of insulin receptor substrate 2 , which mediates the signaling cascades of insulin and insulin like growth factor 1 , leads to a macroautophagy mediated clearance of the accumulated proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Systemic leptin administration to ob / ob mice reverted the diabetic phenotype and improved tissue regeneration as well as the impaired expression of InsR , insulin receptor substrate 1 and insulin receptor substrate 2 , and downstream signaling ( phosphorylation of GS kinase and GS ) in late wounds and nonwounded skin of ob / ob mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Investigating the signaling pathway downstream of IGF 1R reveals that insulin receptor substrate 2 ( IRS 2 ) overexpression compensates for the lack of IGF 1R , whereas IRS 1 overexpression does not . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 ( IRS 2 ) plays a critical role in pancreatic beta cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Basal values for beta cell mass , islet number and proliferation , glucose transporter 2 , glucokinase , and insulin receptor substrate 2 were increased in PBS KO mice ( P < 0 . 05 0 . 001 ) and , although they were increased by Ex 4 treatment of WT animals ( P < 0 . 05 ) , they were decreased in Ex 4 KO mice ( P < 0 . 05 0 . 01 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The results with mice with a neuron specific disruption of the insulin receptor gene or those lacking insulin receptor substrate 2 in hypothalamus supported this possibility . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Epidermal growth factor induces insulin receptor substrate 2 in breast cancer cells via c Jun NH ( 2 ) terminal kinase / activator protein 1 signaling to regulate cell migration . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Ontogenetic characteristics of enzyme activities and plasma metabolites in C57BL / 6J : Jcl mice deficient in insulin receptor substrate 2 . ^^^ We have established an inbred line of mice deficient in insulin receptor substrate 2 ( IRS 2 ) on a C57BL / 6J Jcl genetic background ( B6J IRS 2 ( / ) mice ) as an animal model for typical type 2 diabetes mellitus ( DM ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulated insulin receptor substrate 2 associated phosphatidylinositol 3 kinase activity is enhanced in human skeletal muscle after exercise . ^^^ In mouse skeletal muscle , prior exercise enhances insulin stimulated insulin receptor substrate 2 ( IRS 2 ) signaling ( Diabetes 2002 ; 51 : 479 83 ) , but it is unknown if this also occurs in humans . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Only exercise , through increased proliferation and decreased apoptosis , increased beta cell mass via hyperplasia , which resulted from an enhanced insulin / IGF 1 signaling cascade by insulin receptor substrate 2 induction . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The signaling molecules insulin receptor substrate ( IRS ) 1 and the newly described IRS 2 ( 4PS ) molecule are major insulin and interleukin 4 ( IL 4 ) dependent phosphoproteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We identified pp 190 as a novel substrate for insulin receptor kinase ( IRS 2 ) , which can bind both PI 3 kinase and Ash / Grb2 , and whose tyrosine phosphorylation is specifically induced in IRS 1 deficient mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In all three cell types insulin caused the rapid tyrosine phosphorylation of a 160 kD protein , which was shown not to be the insulin receptor substrate 1 ( IRS 1 ) and may be the recently described IRS 2 , as well as that of a 100 kD polypeptide , which is probably a mixture of the beta subunits of the insulin and insulin like growth factor 1 receptors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Since the activities precipitated by all three antibodies are similar , it seems unlikely that a second insulin receptor substrate , IRS 2 , contributes significantly to the insulin signalling observed in 3T3 L 1 cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Glucose and insulin induced phosphorylation of the insulin receptor and its primary substrates IRS 1 and IRS 2 in rat pancreatic islets . ^^^ Immunoprecipitation with specific antibodies demonstrated that the signal detected at 95 kDa corresponds to the beta subunit of the insulin receptor ( IR ) while the band at 165 185 kDa corresponds to the early substrates of the insulin receptor , IRS 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Stimulation of the insulin receptor ( IR ) results in tyrosine phosphorylation of the intermediate molecules insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , and Shc , which then couple the IR to downstream signaling pathways by serving as binding sites for signaling molecules with SH 2 domains . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To study these pathways , we have used a myeloid progenitor cell line ( 32D ) which is dependent on interleukin 3 but insensitive to insulin because of the very low levels of insulin receptor ( IR ) and the complete lack of insulin receptor substrate ( IRS ) signaling proteins ( IRS 1 and IRS 2 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Recent data suggest involvement of the Janus tyrosine kinase 2 ( JAK 2 ) in human GH induced tyrosine phosphorylation of the GH receptor and the insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) , leading to activation of the phosphatidylinositol 3 kinase and the acute insulin like effects in primary rat adipocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Activated insulin and insulin like growth factor 1 receptors transmit downstream signals via the insulin receptor substrate ( IRS 1 and IRS 2 ) and a series of proteins containing Src homology 2 ( SH 2 ) domains , including SH 2 containing protein tyrosine phosphatase 2 ( SHP 2 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Following injection of AII into rats , we find that there is also a rapid tyrosine phosphorylation of the major insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) in the heart . ^^^ The latter occurs without any reduction in insulin receptor or IRS phosphorylation or in the interaction of the p 85 and p 110 subunits of PI 3 kinase with each other or with IRS 1 / IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin ' s effects are initiated by insulin binding to the insulin receptor , resulting in tyrosine phosphorylation of insulin receptor and intracellular substrates , such as insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , or Shc . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IRS ( insulin receptor substrate ) 1 and IRS 2 were also phosphorylated constitutively in both ovarian cancer cell lines examined , but only the phosphorylation of IRS 1 was augmented in response to IL 4 or IL 13 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Whereas IRS 1 is located on murine chromosome 1 , IRS 2 is located on murine chromosome 8 near the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate ( IRS ) 1 or IRS 2 proteins were not constitutively phosphorylated nor did IL 13 and IL 4 induce phosphorylation of these proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential signaling by insulin receptor substrate 1 ( IRS 1 ) and IRS 2 in IRS 1 deficient cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IL 4 treatment rapidly induced tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 in 1D4 but not in E1C3 cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate ( IRS ) 1 is reduced and IRS 2 is the main docking protein for phosphatidylinositol 3 kinase in adipocytes from subjects with non insulin dependent diabetes mellitus . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The overall architecture of pp 60 is thus the same as that of insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) , and furthermore both the pleckstrin homology and phosphotyrosine binding domains are highly homologous ( about 50 % identical amino acids ) to these domains in both IRS 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Taken together , these observations suggest that IRS 2 plays a unique role in mediating the signals from the insulin receptor to downstream molecules and that this effect is more transient than that of IRS 1 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Pretreatment for about 3 h with dibutyryl cAMP or cAMP generating agents clearly increased insulin dependent tyrosine phosphorylation of the 195 kDa protein , but not IRS 1 , IRS 2 or the insulin receptor beta subunit . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential association of phosphatidylinositol 3 kinase with insulin receptor substrate ( IRS ) 1 and IRS 2 in human thymocytes in response to IL 7 . ^^^ We demonstrate that , in addition to activation of the Jak 1 and Jak 3 kinases , IL 7 stimulation of human thymocytes results in the rapid tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) and IRS 2 proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
By utilizing the insulin receptor substrate ( IRS ) proteins ( IRS 1 and IRS 2 ) , the insulin signal can be amplified or attenuated independently of insulin binding and tyrosine kinase activity , providing an extensible mechanism for signal transmission in multiple cellular backgrounds . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Furthermore , the PH and PTB domains are highly homologous ( at least 40 % identical ) to those found in insulin receptor substrates 1 , 2 , and 3 ( IRS 1 , IRS 2 , and IRS 3 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The ability of insulin to stimulate protein synthesis and cellular growth is mediated through the insulin receptor ( IR ) , which phosphorylates Tyr residues in the insulin receptor substrate signaling proteins ( IRS 1 and IRS 2 ) , Gab 1 , and Shc . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Following phosphorylation by the insulin receptor kinase , the insulin receptor substrates ( IRS ) 1 and IRS 2 bind to and activate several Src homology 2 ( SH 2 ) domain proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin binding to its receptor induces the phosphorylation of cytosolic substrates , insulin receptor substrate ( IRS ) 1 and IRS 2 , which associate with several Src homology 2 domain containing proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin is thought to activate this enzyme by stimulating its association with tyrosine phosphorylated proteins such as insulin receptor substrate ( IRS ) 1 , IRS 2 , Grb 2 associated binder 1 , and pp 60 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Elevated serine / threonine phosphorylation of IRS 1 and IRS 2 inhibits their binding to the juxtamembrane region of the insulin receptor and impairs their ability to undergo insulin induced tyrosine phosphorylation . ^^^ Tumor necrosis factor alpha ( TNFalpha ) or chronic hyperinsulinemia that induce insulin resistance trigger increased Ser / Thr phosphorylation of the insulin receptor ( IR ) and of its major insulin receptor substrates , IRS 1 and IRS 2 . ^^^ To unravel the molecular basis for this uncoupling in insulin signaling , we undertook to study the interaction of Ser / Thr phosphorylated IRS 1 and IRS 2 with the insulin receptor . ^^^ Such impaired interactions abolish the ability of IRS 1 and IRS 2 to undergo insulin induced Tyr phosphorylation and further propagate the insulin receptor signal . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Activated insulin receptor ( IR ) interacts with its substrates , IRS 1 , IRS 2 , and Shc via the NPXY motif centered at Y 960 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate ( IRS ) 1 and IRS 2 are tyrosine phosphorylated and associated with phosphatidylinositol 3 kinase in response to brain derived neurotrophic factor in cultured cerebral cortical neurons . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential regulation of insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) and phosphatidylinositol 3 kinase isoforms in liver and muscle of the obese diabetic ( ob / ob ) mouse . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To elucidate the mechanism of obesity related insulin resistance , we investigated the impaired steps in the processes of phosphatidylinositol ( PI ) 3 kinase activation through binding with insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) in liver and muscle of Zucker fatty rats . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Moreover , they were able to phosphorylate in vitro native insulin receptor substrate ( IRS ) 1 and a fragment of IRS 2 ( GST IRS 2591 786 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
However , insulin receptor substrate ( IRS ) 1 and IRS 2 signaling in response to insulin showed functional differences . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulation results in a considerable spectrum of cellular responses , only part of which have been firmly correlated with the activation of established insulin receptor ( IR ) targets such as IRS 1 , IRS 2 , and Shc . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Signaling of IGFs occurs through insulin receptor substrate ( IRS ) 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of the insulin receptor ( IR ) , insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) , and pp 60 , and phosphatidylinositol ( PI ) 3 kinase activity ( using PI as substrate ) and mitogen activated protein kinase ( MAPK ) activity were assayed in cell lysates . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Because insulin like growth factor 1 ( IGF 1 ) , insulin , and interleukin 4 ( IL 4 ) have known biological effects in breast cancer cells and signal through insulin receptor substrate ( IRS ) adaptor proteins , we examined the expression and function of IRS 1 and IRS 2 in breast tumors and cell lines . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential compartmentalization and trafficking of insulin receptor substrate ( IRS ) 1 and IRS 2 . ^^^ We find that insulin receptor substrate ( IRS ) 1 is 2 fold more concentrated in the intracellular membrane ( IM ) compartment than in cytosol , whereas IRS 2 is 2 fold more concentrated in cytosol than in IM . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate ( IRS ) proteins IRS 1 and IRS 2 differential signaling in the insulin / insulin like growth factor 1 pathways in fetal brown adipocytes . ^^^ In the present study we have investigated the contribution of the insulin receptor substrate proteins ( IRS 1 and IRS 2 ) to the insulin / insulin like growth factor 1 ( IGF 1 ) signaling pathways in fetal rat brown adipocytes , a model that expresses both insulin and IGF 1 receptors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Using specific antibodies , we now show that GH was also able to induce tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 / IRS 2 in liver . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Although the mitogenic function of the IGF IR may require the activation of insulin receptor substrate 1 ( IRS 1 ) or IRS 2 , an overexpressed IGF IR is able to protect 32D cells , which lack IRS 1 and IRS 2 , from apoptosis caused by Interleukin 3 ( IL 3 ) withdrawal . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The expression of a number of genes encoding key players in insulin signalling and action , including insulin , insulin receptor ( IR ) , downstream signalling molecules such as insulin receptor substrate 1 ( IRS 1 ) and IRS 2 , glucose transporters ( GLUT 4 , GLUT 2 ) and important metabolic enzymes such as glucokinase , has now been altered in transgenic or knockout mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Different subcellular distribution and regulation of expression of insulin receptor substrate ( IRS ) 3 from those of IRS 1 and IRS 2 . ^^^ Adipocytes contain three major substrate proteins of the insulin receptor , termed IRS 1 , IRS 2 , and IRS 3 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The five compounds elicited in rat adipocytes a significant increase in tyrosine phosphorylation of both insulin receptor substrate 1 ( IRS 1 ) and IRS 3 and , to a minor degree , IRS 2 , in IRS 1 / 3 associated phosphatidylinositol 3 kinase ( PI 3 K ) protein as well as activity , and in protein kinase B ( PKB ) activity as well as phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
These results suggest that the binding of acidic motifs to the PH domain of IRS 1 and IRS 2 disrupts coupling to the activated insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin action starts with binding to a membrane receptor ( insulin receptor tyrosine kinase ) and with activating an insulin receptor substrate 1 ( IRS 1 ) and substrate 2 ( IRS 2 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In cultured cells , GH stimulates the tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , and Shc . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate ( IRS ) 1 and IRS 2 , which mediate phosphatidylinositol ( PI ) 3 kinase activation , play essential roles in insulin induced translocation of GLUT 4 and in glycogen synthesis . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Differential role of insulin receptor substrate ( IRS ) 1 and IRS 2 in L 6 skeletal muscle cells expressing the Arg 1152 > Gln insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin rapidly stimulated tyrosine phosphorylation of the insulin receptor , insulin receptor substrate 1 ( IRS 1 ) and , to a lesser extent , IRS 2 in normal and diabetic myocardium . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Compared with controls , diabetic rats were characterized by multiple insulin signaling abnormalities in skeletal muscle , which included 1 ) increased insulin stimulated tyrosine phosphorylation of the insulin receptor beta subunit and insulin receptor substrates IRS 1 and IRS 2 , 2 ) increased substrate tyrosine phosphorylation in the basal state , 3 ) a decreased amount of IRS 1 protein , 4 ) markedly elevated basal and insulin stimulated phosphatidylinositol ( PI ) 3 kinase activity in anti IRS 1 immunoprecipitates from total tissue extracts , and 5 ) increased PI 3 kinase activity in low density microsomes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin signalling cascade from the insulin receptor to PI 3 K was also found to be abnormal , resulting in a severely reduced phosphorylation degree of the IRS 1 ( IRS 2 ? ) PI 3 K complex , which can explain both reduced glucose transport and glycogen synthesis . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We show here that estrogen enhances IGF signaling by inducing expression of three key IGF regulatory molecules , the type 1 IGF receptor ( IGFR 1 ) and its downstream signaling molecules , insulin receptor substrate ( IRS ) 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To determine whether glucosamine induced insulin resistance could be due to impaired signaling , we measured insulin receptor substrate ( IRS ) 1 and insulin receptor tyrosine phosphorylation ; PI 3 kinase activity associated with IRS 1 , IRS 2 , and phosphotyrosine ; and Akt activity and phosphorylation in skeletal muscle of rats infused for 2 h with glucosamine ( 6 . 0 mg 10 kg ( 1 ) 10 min ( 1 ) ) or saline . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
It also stimulates the tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 , indicating that both proteins serve as substrates of IRR protein tyrosine kinase in intact cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Genetic ablation experiments of insulin receptor substrate 1 ( IRS 1 ) and 2 ( IRS 2 ) , two important molecules in the IR and IGF IR signaling pathways , are also beginning to shed light onto the mechanisms accounting for the specificity of IR and IGF IR signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin induced tyrosine phosphorylation of insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) and their protein levels were decreased in the aorta of obese rats compared with lean rats . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The high glucose concentration also activated the insulin receptor kinase toward the endogenous insulin receptor substrates ( IRS ) 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In response to insulin , tyrosine kinase activity of the insulin receptor is stimulated , leading to autophosphorylation and tyrosine phosphorylation of proteins including insulin receptor subunit ( IRS ) 1 , IRS 2 , and Shc . ^^^ After 1 min of insulin stimulation , the insulin receptor was tyrosine phosphorylated 8 fold more and Shc was phosphorylated 50 % less in 32D cells containing both IRS 1 and insulin receptors ( 32D / IR+IRS 1 ) than in 32D cells containing only insulin receptors ( 32D / IR ) , insulin receptors and IRS 2 ( 32D / IR+IRS 2 ) , or insulin receptors and a form of IRS 1 that can not be phosphorylated on tyrosine residues ( 32D / IR+IRS 1F18 ) . ^^^ Therefore , IRS 1 and IRS 2 appeared to have different effects on insulin receptor phosphorylation and downstream signaling . ^^^ This effect of IRS 1 on insulin receptor phosphorylation is unique to IRS 1 , as IRS 2 had no effect on insulin receptor tyrosine phosphorylation . ^^^ Therefore , IRS 1 and IRS 2 appear to function differently in their effects on signaling downstream of the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin induced tyrosine phosphorylation of the insulin receptor , insulin receptor substrate 1 ( IRS 1 ) , and IRS 2 was reduced by prestimulation of beta ( 3 ) adrenergic receptors ( CL 316243 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Recent reports have suggested that leptin can act through some of the components of the insulin signaling cascade , such as insulin receptor substrates ( IRS 1 and IRS 2 ) , phosphatidylinositol 3 kinase ( PI 3 kinase ) , and mitogen activated protein kinase , and can modify insulin induced changes in gene expression in vitro and in vivo . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Initial signalling events of insulin action , including receptor kinase activation , the tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 and the recruitment of PI 3K to IRS 1 and IRS 2 , were found not to be involved in contraction mediated signalling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We further show that in SFFV infected erythroid cells grown in the absence of Epo , PI 3 kinase associates with the insulin receptor substrate ( IRS ) related adapter molecules IRS 2 , Gab 1 , and Gab 2 , which are constitutively tyrosine phosphorylated in SFFV infected cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In the present study we have investigated the effect of increased serine / threonine phosphorylation of insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) by okadaic acid pretreatment on brown adipocyte insulin signalling leading to glucose transport , an important metabolic effect of insulin in brown adipose tissue . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We have previously shown that IRS 1 and IRS 2 interact with the juxtamembrane ( JM ) but not with the carboxyl terminal ( CT ) region of the insulin receptor ( IR ) in vitro . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Glucose ( > 3 mM ) independently increased tyrosine phosphorylation mediated recruitment of growth factor bound protein 2 ( Grb 2 ) / murine sons of sevenless 1 protein ( mSOS ) and PI3 ' K to insulin receptor substrate ( IRS ) 1 and IRS 2 , as well as SH 2 containing protein ( Shc ) association with Grb2 / mSOS and downstream activation of mitogen activated protein kinase and 70 kDa S 6 kinase . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In order to distinguish common and unique functions of IRS 1 , IRS 2 , and IRS 4 , we expressed them individually in 32D myeloid progenitor cells containing the human insulin receptor ( 32D ( IR ) ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin increased insulin receptor substrate ( IRS ) 1 and IRS 2 tyrosine phosphorylation and phosphotyrosine associated phosphatidylinositol ( PI ) 3 kinase activity to a maximal level after 3 10 min , with subsequent downregulation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Treatment with H 7 did not affect IL 4R mediated immediate signaling events such as tyrosine phosphorylation of Jak 1 , Jak 3 , insulin receptor substrate ( IRS ) 1 and IRS 2 , or tyrosine phosphorylation and DNA binding of Stat 6 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Tissue specific insulin resistance in mice with mutations in the insulin receptor , IRS 1 , and IRS 2 . ^^^ To analyze the role of the insulin signaling pathway in these processes , we have generated mice with combined heterozygous null mutations in insulin receptor ( ir ) , insulin receptor substrate ( irs 1 ) , and / or irs 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We studied the impact of 16 serine residues in HIR by mutation to alanine and co overexpression in human embryonic kidney ( HEK ) 293 cells together with the docking proteins insulin receptor substrate ( IRS ) 1 , IRS 2 , or ( SHC ) Src homologous and collagen like . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Aberrant protein expression can not account for these insulin signaling defects because expression of insulin receptor , IRS 1 , IRS 2 , MAP kinase , or glycogen synthase was similar between type 2 diabetic and control subjects . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Genotype phenotype correlations in first degree relatives of an index case caring the Type A syndrome , suggested that association of allelic variants of IRS 1 and IRS 2 with insulin receptor mutations contribute , by synergistic effects , to phenotypic expression of defects in signal transduction . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulates the tyrosine kinase activity of its receptor resulting in the tyrosine phosphorylation of pp 185 , which contains insulin receptor substrates IRS 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 3 ( IRS 3 ) and IRS 4 impair IRS 1 and IRS 2 mediated signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The most widely distributed members of the family of insulin receptor substrate ( IRS ) proteins are IRS 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Essentially no p 85 alpha subunit of PI 3 kinase was found to be associated with insulin receptor substrate ( IRS ) 1 or IRS 2 in basal and insulin stimulated ( 5 min ) cardiomyocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Short term incubation of adult rat hepatocytes with epidermal growth factor ( EGF ) caused tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 when the cells had been submitted to primary culture from 1 18 h . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
These responses are largely mediated downstream of the insulin receptor substrates , IRS 1 and IRS 2 ( refs 4 8 ) , through distinct signalling pathways . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin and IGF 1 stimulated PI 3 K activity was mainly associated with insulin receptor substrate ( IRS ) isoforms IRS 1 and IRS 2 and less so with the IRS isoform Grb 2 associated binder 1 ( Gab 1 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Four members ( IRS 1 , IRS 2 , IRS 3 and IRS 4 ) of this family have been identified that differ in tissue distribution , subcellular localisation , developmental expression , binding to the insulin receptor and interaction with SH 2 domain containing proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To determine effector functions of SNT that promote neuronal differentiation of PC 12 pheochromocytoma cells , we engineered a chimeric protein , SNT 1 ( IRS ) CX , bearing the effector region of SNT 1 and the insulin receptor recognition domains of IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : In liver of rats with sustained endotoxemia , there were significant decreases in insulin stimulated tyrosine phosphorylation of insulin receptors ( 74 % ) , insulin receptor substrate ( IRS ) 1 ( 74 % ) , and IRS 2 ( 53 % ) ; binding of the p 85 subunit of phosphatidylinositide 3 kinase to IRS 1 ( 80 % ) ; and IRS 1 precipitable phosphatidylinositide 3 kinase activity ( > 90 % ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Biochem . 268 , 25 34 ] , epidermal growth factor ( EGF ) gave rise to transient tyrosine phosphorylation of insulin receptor substrates ( IRS 1 and IRS 2 ) , thereby activating the bound phosphatidylinositol 3 kinase in human epidermoid carcinoma A 431 cells normally abundant in EGF receptors ( EGFR ) and Chinese hamster ovary ( CHO ) cells transfected with full length EGFR . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To assess the role of insulin receptor , insulin receptor substrate ( IRS ) 1 , and IRS 2 genes in insulin resistance , we explored the genomic DNA in women with polycystic ovary syndrome ( PCOS ) and a variable degree ( mean + / SE ) of insulin resistance ( homeostasis model assessment index for insulin resistance [ HOMA ( IR ) ] 3 . 2 + / 0 . 6 , n = 53 ; control subjects 1 . 56 + / 0 . 34 , n = 102 ) using direct sequencing . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This was paralleled by a slight reduction in the insulin induced tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We examined the abundance and degree of tyrosine phosphorylation of the insulin receptor ( IR ) , insulin receptor substrate 1 ( IRS 1 ) , and IRS 2 in skeletal muscle and , for comparison , liver . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Preincubation of cells with leptin also had no effect on insulin stimulated tyrosine phosphorylation of insulin receptor , IRS 1 and IRS 2 , phosphatidylinositol 3 kinase activity , or Akt phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Four members ( IRS 1 , IRS 2 , IRS 3 , IRS 4 ) of this family have been identified that differ as to tissue distribution , subcellular localization , developmental expression , binding to the insulin receptor , and interaction with SH 2 domain containing proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Western blot analysis revealed no significant differences in the amount of insulin receptor ( IR ) , insulin receptor substrates 1 and 2 ( IRS 1 , IRS 2 ) , and the p 85 subunit of phosphatidylinositol ( PI ) 3 kinase . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
However , the estrogen receptor ( ER ) content remained similar in tumors from both groups , at least at the protein level , and so did the expression of IGF 1 , IGF 2 , insulin receptor substrate 1 ( IRS 1 ) , IRS 2 and epidermal growth factor receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulation of insulin receptor substrate ( IRS ) 1 , IRS 2 , and antiphosphotyrosine associated phosphatidylinositol 3 kinase ( PI3K ) was similar for CR and AL . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Down regulation of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc gene expression by insulin in skeletal muscle is not associated with insulin resistance or type 2 diabetes . ^^^ To examine whether altered gene expression of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc is an inherited trait and is associated with muscle insulin resistance or type 2 diabetes , we measured mRNA levels of these genes by a relative quantitative RT PCR method in muscle biopsies taken before and after an insulin clamp from 12 monozygotic twin pairs discordant for type 2 diabetes and 12 control subjects . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Because the increase in PI 3 kinase activity can not be explained by increased insulin receptor substrate ( IRS ) 1 signaling , the present study examined whether this effect is mediated by enhanced IRS 2 signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
After the culture period , the cells were washed and then basal and insulin stimulated glucose uptake , insulin binding and lipolysis as well as cellular content of insulin signalling proteins ( insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , phosphatidylinositol 3 kinase ( PI 3 K ) and protein kinase B ( PKB ) ) and glucose transporter isoform GLUT 4 were measured . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This insulin resistance was associated with impaired insulin receptor substrate ( IRS ) 2 associated phosphatidylinositol 3 ' ( PI 3 ) kinase activation and IRS 2 tyrosine phosphorylation as well as significantly decreased protein kinase C ( PKC ) zeta / lambda activation in response to insulin . ^^^ Protein expression levels for the insulin receptor , IRS 1 , IRS 2 , the p 85 regulatory subunit of PI 3 kinase , Akt , PKC zeta / lambda , GLUT 1 , and GLUT 4 were also similar in the two groups . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Increased insulin sensitivity and upregulation of insulin receptor , insulin receptor substrate ( IRS ) 1 and IRS 2 in liver of Ames dwarf mice . ^^^ Insulin stimulated phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 was increased by 61 and 72 % respectively , while IRS 1 and IRS 2 protein levels were increased by 76 and 95 % . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulated phosphorylation of the insulin receptor , insulin receptor substrate ( IRS ) 1 , and associated phosphatidylinositol 3 kinase were normal in TG mice , whereas IRS 2 protein and phosphorylation were down regulated compared with control mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Overexpression of an NH ( 2 ) terminal fragment of IRS 1 that contains the pleckstrin homology and phosphotyrosine binding domains ( insulin receptor substrate 1 NH ( 2 ) terminal fragment [ IRS 1N ] ) inhibited insulin induced tyrosine phosphorylation of IRS 1 as well as the association of IRS 1 with phosphatidylinositol ( PI ) 3 kinase activity , whereas the tyrosine phosphorylation of IRS 2 and its association with PI 3 kinase activity were slightly enhanced . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Positive and negative regulatory role of insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) serine / threonine phosphorylation . ^^^ To determine whether basal Ser / Thr phosphorylation of IRS proteins influences insulin receptor catalyzed tyrosine phosphorylation , recombinant FLAG epitope tagged IRS 1 ( F IRS 1 ) and IRS 2 ( F IRS 2 ) were expressed , purified , and subjected to both dephosphorylation and hyperphosphorylation prior to phosphorylation by the insulin receptor kinase . ^^^ As expected , hyperphosphorylation of F IRS 1 and F IRS 2 by GSK3beta decreased their subsequent phosphorylation on tyrosine residues by the insulin receptor . ^^^ Surprisingly , however , dephosphorylation of the basal Ser / Thr phosphorylation sites impaired subsequent phosphorylation on tyrosine , suggesting that basal Ser / Thr phosphorylation of F IRS 1 and F IRS 2 plays a positive role in phosphorylation by the insulin receptor tyrosine kinase . ^^^ IRS 1 and IRS 2 from serum fed cells were hyperphosphorylated , and dephosphorylation induced either by serum deprivation or by alkaline phosphatase treatment after immunoprecipitation led to an increase in tyrosine phosphorylation by the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Rosiglitazone ameliorated the defect in hepatocyte insulin stimulated tyrosine phosphorylation of the insulin receptor , IRS 1 , and IRS 2 and the reduced protein mass of IRS 1 and IRS 2 induced by fructose feeding . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In addition , these SH 2 domains interacted with a protein complex consisting of insulin receptor substrate 4 ( IRS 4 ) , IRS 2 , and the p 85 regulatory subunit of phosphatidylinositol 3 kinase . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Nuclear translocation of insulin receptor substrate 1 by the simian virus 40 T antigen and the activated type 1 insulin like growth factor receptor . 32D cells are a murine hemopoietic cell line that undergoes apoptosis upon withdrawal of interleukin 3 ( IL 3 ) from the medium . 32D cells have low levels of the type 1 insulin like growth factor ( IGF 1 ) receptor and do not express insulin receptor substrate 1 ( IRS 1 ) or IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In this study , we show that insulin receptor substrate ( IRS ) 1 , IRS 2 , and signal transducer and activator of transcription 6 ( STAT 6 ) are phosphorylated following IL 4 treatment in MCF 7 breast cancer cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
After receptor binding , growth hormone ( GH ) induces GH receptors ( GHR ) dimerization and JAK 2 is activated after its association with a dimerized GHR , stimulating the tyrosyl phosphorylation of insulin receptor substrate 1 ( IRS 1 ) , IRS 2 and Shc proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Igf1r haploinsufficiency did not affect insulin action and compensatory beta cell growth in insulin resistant mice with combined Insr and Igf1r heterozygous null mutations , resulting in a considerably milder phenotype than combined haploinsufficiency for Insr and its main signaling substrates , Irs 1 and Irs 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Lactate infusion did not alter insulin ' s ability to phosphorylate the insulin receptor , the insulin receptor substrate ( IRS ) 1 , or IRS 2 but decreased insulin ' s ability to stimulate IRS 1 and IRS 2 associated phosphatidylinositol 3 kinase activities and Akt / protein kinase B activity by 47 , 75 , and 55 % , respectively ( P < 0 . 05 for all ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
EGF increased the tyrosine phosphorylation of several proteins ( the EGF receptor , insulin receptor substrate ( IRS ) 1 , IRS 2 , and Grb 2 associated binder 1 ) , whereas Shc and Gab 2 were only weakly and inconsistently phosphorylated . p 85 , the regulatory subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) , was also found to associate with all of these docking molecules , showing that EGF activated PI 3 kinase pools that were additional to those of insulin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The expression of insulin receptor and IRS 1 and IRS 2 was decreased in obese IRS 1 ( + / ) , which could in part explain the profound insulin resistance in these mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In the biopsies , insulin receptor kinase ( IRK ) activity , insulin receptor substrate ( IRS ) 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity , Ser ( 473 ) and Thr ( 308 ) phosphorylation of protein kinase B ( PKB ) , and protein expression of IRS 1 , IRS 2 , phosphoinositol dependent kinase 1 ( PDK 1 ) , PKB , and GLUT 4 were determined . ^^^ Troglitazone did not alter insulin receptor number or IRS 1 , IRS 2 , PKB , PDK 1 , or GLUT 4 protein expression . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In contrast , no effect was detected on the insulin receptor and IRS 2 tyrosine phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Polymorphisms in the genes encoding the insulin receptor substrate ( IRS ) proteins , IRS 1 ( Gly ( 972 ) Arg ) and IRS 2 ( Gly ( 1057 ) Asp ) , influence susceptibility to type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin stimulated association of phosphatidylinositol 3 kinase with insulin receptor substrates 1 and 2 was increased with dexamethasone , the increased association with IRS 2 may , at least partially , be explained by higher IRS 2 protein expression . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In the present experiments we demonstrate that higher insulin receptor and insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) concentrations are predominantly encountered in cells of the periphery of rat pancreatic islets , as compared to centrally located cells , and that partial blockade of IRS 1 protein expression by antisense oligonucleotide treatment leads to improved insulin secretion induced by glucose overload , which is accompanied by lower steady state glucagon secretion and blunted glucose induced glucagon fall . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Short term denervation induced a significant fall in glucose clearance rates ( 62 % of control , P < 0 . 05 ) as detected by euglycemic hyperinsulinemic clamp and was associated with a significant decrease in insulin stimulated tyrosine phosphorylation of the insulin receptor ( IR ; 73 % of control , P < 0 . 05 ) , IR substrate 1 ( IRS 1 ; 69 % of control , P < 0 . 05 ) , and IRS 2 ( 82 % of control , P < 0 . 05 ) and serine phosphorylation of Akt ( 39 % of control , P < 0 . 05 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In two cohorts of 174 and 165 obese Caucasian children , we measured insulin sensitivity and genotyped insulin receptor substrate IRS 1 and IRS 2 genes for the Arg972Gly and the Asp1057Gly variants , respectively . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Grb 10 inhibits insulin stimulated insulin receptor substrate ( IRS ) phosphatidylinositol 3 kinase / Akt signaling pathway by disrupting the association of IRS 1 / IRS 2 with the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Impact of genetic background and ablation of insulin receptor substrate ( IRS ) 3 on IRS 2 knock out mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Based on the phenotypes of knockout mice and cell lines , as well as pathway specific analysis , the insulin receptor substrates IRS 1 , IRS 2 , IRS 3 , and IRS 4 have been shown to play unique roles in insulin signal transduction . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Hyperosmolarity induces sustained suppression of p70S6 kinase and 4E BP 1 hyperphosphorylation by insulin , whereas insulin induced tyrosine phosphorylation of the insulin receptor ( IR ) beta subunit and the IR substrates IRS 1 and IRS 2 , recruitment of the phosphoinositide 3 kinase ( PI 3 kinase ) regulatory subunit p 85 to the receptor substrates as well as PI 3 kinase activation , and Ser 473 phosphorylation of protein kinase B and Thr 410 / 403 phosphorylation of PKC zeta / lambda are largely unaffected under hyperosmotic conditions . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
At high glucose , insulin receptor substrate 1 ( IRS 1 ) expression was downregulated by approximately 20 50 % , whereas IRS 2 was strongly upregulated by glucose levels of 10 mmol / l or more ( by 100 400 % ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The MEK 1 mutant , which activates ERK , markedly down regulated expression of the insulin receptor ( IR ) and its major substrates , IRS 1 and IRS 2 , mRNA and protein , and in turn reduced tyrosine phosphorylation of IR as well as IRS 1 and IRS 2 and their associated phosphatidyl inositol 3 kinase ( PI3K ) activity . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Finally , induction of insulin receptor substrate ( IRS ) 1 in the mammary glands of PRL treated mice and induction of IRS 1 and IRS 2 after treatment with PRL plus progesterone indicates that these molecules are induced by PRL as potential signaling intermediates downstream from IGF I / insulin receptors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 2 deficient ( IRS 2 ( / ) ) mice develop type 2 diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Desensitization was correlated to a reduction in insulin receptor substrate ( IRS ) 1 and IRS 2 protein levels , which was reversed by the PI3K inhibitor LY 294002 . ^^^ In summary , ( 1 ) PI3K mediates insulin induced reduction of IRS 1 by phosphorylating it while a PI3K / mTOR pathway controls insulin induced reduction of IRS 2 , ( 2 ) in L 6 cells , IRS 2 is the major adapter molecule linking the insulin receptor to activation of PKB and MAPK , ( 3 ) the mechanism of IRS 1 / 2 down regulation is different in L 6 cells compared with 3T3 L 1 adipocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Finally , we tested common polymorphisms in insulin receptor substrate ( IRS ) 1 , IRS 2 , calpain 10 , hepatic lipase , and peroxisome proliferator activated receptor gamma for association with the shape index . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Because the other known PH PTB proteins ( insulin receptor substrates : IRS 1 , IRS 2 , IRS 3 , and IRS 4 , and the downstream of kinases : DOK 1 , DOK 2 , and DOK 3 ) are substrates of insulin and insulin like growth factor ( IGF ) 1 receptors , we asked whether these new proteins , termed IRS5 / DOK4 and IRS6 / DOK5 , might also have roles in insulin and IGF 1 signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The ability of GH to stimulate lipogenesis and tyrosine phosphorylation of the GH receptor ( GHR ) , Janus kinase 2 ( Jak 2 ) , insulin receptor substrate 1 ( IRS 1 ) and 2 ( IRS 2 ) was greatly reduced in refractory as compared to responsive primary rat adipocytes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Instead , TNFalpha significantly reduces by approximately 50 % IGF 1 stimulated tyrosine phosphorylation of two of the major downstream receptor docking molecules , insulin receptor substrate ( IRS ) 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The purpose of this study was to determine the roles of the insulin receptor substrate ( IRS 1 and IRS 2 ) mediated growth cascades in rapidly growing fetal rat liver . ^^^ METHODS : We determined the expression and tyrosyl phosphorylation of the insulin receptor beta subunit ( IRbeta ) , IRS 1 and IRS 2 , the binding of phosphatidylinositol 3 kinase ( PI3K ) , and activation of the mitogen activated protein kinase ( MAPK ) pathway in the presence or absence of insulin stimulation in vivo during development and in the adult liver . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Furthermore , PDGF stimulates these effects independent of insulin receptor substrate ( IRS ) 1 or IRS 2 tyrosine phosphorylation or docking to activated PI3K . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
To assess the role of insulin receptor ( IR ) substrate ( IRS ) 2 in insulin action and resistance in the liver , immortalized neonatal hepatocyte cell lines have been generated from IRS 2 ( / ) , IRS 2 ( + / ) , and wild type mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This effect of IL 6 was accompanied by a marked reduction in IRS 1 , but not IRS 2 , protein expression , and insulin stimulated tyrosine phosphorylation , whereas no inhibitory effect was seen on the insulin receptor tyrosine phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) , two major downstream molecules of IGF 1R , are known to be important in the genesis of diabetes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Drugs that stimulate IRS 2 ( insulin receptor substrate protein 2 ) synthesis or signaling might be a good starting point . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In 3T3 L 1 adipocytes , DHEA increases tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 and stimulates IRS 1 and IRS 2 associated phosphatidylinositol ( PI ) 3 kinase activity with no effects on either insulin receptor or Akt phosphorylation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin signals are mediated by tyrosine phosphorylation of the insulin receptor and its downstream targets , such as Irs 1 and Irs 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
METHODS : Insulin stimulated phosphorylation of insulin receptor ( IR beta ) , insulin receptor substrates ( IRS 1 and IRS 2 ) , association of phosphatidylinositol 3 kinase ( PI 3 kinase ) , Ser 473 phosphorylation of Akt and Thr202 / Tyr204 phosphorylated p44 / 42 MAP kinase in 3T3L1 adipocytes and FAO hepatoma cells incubated with increasing concentrations of saquinavir for 24 , 36 hours , 2 , 3 and 6 days were measured . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We previously reported that insulin receptor substrate 2 ( IRS 2 ) deficient mice develop diabetes as a result of insulin resistance in the liver and failure of beta cell hyperplasia . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) : what a tangled web we weave . ^^^ The insulin receptor is a transmembrane tyrosine kinase that is essential for mediating multiple intracellular signalling cascades that lead ultimately to the biological actions of insulin Tyrosine phosphorylation o f the cytosolic proteins insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) produces protein ' scaffolding ' for the assembly of effector proteins containing Src homology 2 ( SH 2 ) domains , thereby generating multisubunit signalling complexes . ^^^ Although IRS 1 was originally isolated as a specific insulin receptor substrate , both IRS 1 and IRS 2 appear to play a broader role , functioning also as proximal substrates in growth hormone and cytokine receptor signalling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Systemic deletion of SH 2 B impaired insulin receptor activation and signaling in the liver , skeletal muscle , and fat , including tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) and IRS 2 and activation of the phosphatidylinositol 3 kinase / Akt and the Erk1 / 2 pathways . ^^^ Moreover , SH 2 B directly enhanced autophosphorylation of insulin receptor and tyrosine phosphorylation of IRS 1 and IRS 2 in an SH 2 domain dependent manner in cultured cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
AngII induced Janus kinase 2 tyrosine phosphorylation and coimmunoprecipitation with insulin receptor substrate 1 ( IRS 1 ) and IRS 2 as well as an increase in tyrosine phosphorylation of IRS and its association with growth factor receptor binding protein 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
SH 2 B promotes insulin receptor substrate 1 ( IRS 1 ) and IRS 2 mediated activation of the phosphatidylinositol 3 kinase pathway in response to leptin . ^^^ Here we report that SH 2 B , a JAK 2 interacting protein , promotes activation of the PI 3 kinase pathway by recruiting insulin receptor substrate 1 ( IRS 1 ) and IRS 2 in response to leptin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
There was no change in the protein expression of insulin receptor beta subunit ( IR beta ) , insulin receptor substrate 1 ( IRS 1 ) , IRS 2 , the p 85 subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) , Akt , and glucose transporter protein 4 ( GLUT 4 ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Stable transfection of murine 32D myeloid cells ( which lack endogenous IRS proteins and are insensitive to growth inhibition by IGFBP 3 ) with IRS 1 or IRS 2 cDNA confers sensitivity to growth inhibition by IGFBP 3 ; this IRS mediated growth inhibition can be completely reversed by insulin in 32D cells stably expressing IRS 2 and the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This is the result of downregulation of the insulin receptor substrates , IRS 1 and IRS 2 , which become limiting for signal transmission from the insulin receptor to PI3K . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Here we found that , in brown adipose tissue ( BAT ) , another insulin sensitive tissue , the basal tyrosine phosphorylations of insulin receptor ( IR ) and IR substrates ( IRS 1 and IRS 2 ) were upregulated in SCD 1 ( / ) mice compared with wild type mice . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In the present study , we examined the protein levels and phosphorylation state of insulin receptor ( IR ) , IRS 1 and IRS 2 , the association between IRSs and PI3K and SHP 2 , the insulin induced IRSs associated PI 3 kinase activities , and the phosphorylation status of AKT and atypical PKCzeta / lambda in the liver and the muscle of 6 month old Wistar rats treated with DHEA . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
An increase in fasting insulin levels was associated with the presence of serum HCV core , the severity of hepatic fibrosis and a decrease in expression of insulin receptor substrate ( IRS ) 1 and IRS 2 , central molecules of the insulin signaling cascade , in patients with HCV infection . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Once induced , SOCS 3 interacts with the insulin receptor , JAK 2 , IRS 1 , and IRS 2 . ^^^ The inhibition of SOCS 3 expression by a phosphorthioate modified antisense oligonucleotide partially restores angiotensin 2 induced inhibition of insulin induced insulin receptor , IRS 1 and IRS 2 tyrosine phosphorylation , and IRS 1 and IRS 2 association with p 85 phosphatidylinositol 3 kinase and [ Ser 473 ] phosphorylation of Akt . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Interestingly , those patients revealed an impaired insulin mediated insulin receptor substrate ( IRS ) 1 binding to p 85 alpha without any alteration in IRS 2 / p85 alpha association . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Although insulin receptor substrate 1 ( IRS 1 ) and IRS 2 , among other factors , activate PI3K , there is little information on the relative roles of IRS 1and IRS 2 during aPKC activation by insulin action in specific cell types . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
CYP2E1 overexpression in a hepatocyte cell line decreased tyrosine phosphorylation of insulin receptor substrate ( IRS ) 1 and IRS 2 in response to insulin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Because activation of PKB can require insulin receptor substrate proteins ( IRS 1 and IRS 2 ) and phosphatidylinositol 3 kinase ( PI3K ) , it is of interest to determine whether the activity of Foxo 1 is also regulated by heterotrimeric G protein coupled receptors ( GPCRs ) with IRS 1 or 2 , PI3K , or PKB signaling potential . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In many cases , insulin resistance in liver is associated with reduced expression of both major insulin receptor substrate ( IRS ) proteins , IRS 1 and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
We have also showed that insulin and leptin are able to activate PI3K through IRS 1 ( insulin receptor substrate 1 ) and IRS 2 respectively . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
This study initially confirmed in mouse osteoblast cultures that PTH treatment increased IGF 1 mRNA and protein levels and alkaline phosphatase activity , which were accompanied by phosphorylations of IGF 1 receptor , insulin receptor substrate ( IRS ) 1 and IRS 2 , essential adaptor molecules for the IGF 1 signaling . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
As compared with control , PCOS granulosa cells had higher insulin receptor substrate 1 ( IRS 1 ) expression , but lower IRS 2 expression . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrates 1 and 2 ( IRS 1 and IRS 2 ) are pivotal in relaying insulin signaling in insulin responsive tissues such as muscle . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The insulin like effects are mediated by the cytosolic tyrosine kinase Janus kinase 2 ( JAK 2 ) upon GH GH receptor interaction , resulting in tyrosine phosphorylation of downstream targets including the GH receptor itself and insulin receptor substrate 1 ( IRS 1 ) and IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrate 1 ( IRS 1 ) and IRS 2 are known to transduce and amplify signals emanating from the insulin receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Alterations in muscle and adipose tissue insulin receptor substrate ( IRS ) 1 and IRS 2 are associated with , and commonly believed to contribute to , development of insulin resistance . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Biochemical analyses demonstrated that chronic Akt activation induces feedback inhibition of PI3K activity through both proteasome dependent degradation of insulin receptor substrate 1 ( IRS 1 ) and inhibition of transcription of IRS 1 as well as that of IRS 2 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Using oligonucleotide microarrays and real time PCR of pancreatic islets isolated from humans with type 2 diabetes versus normal glucose tolerant controls , we identified multiple changes in expression of genes known to be important in beta cell function , including major decreases in expression of HNF4alpha , insulin receptor , IRS 2 , Akt 2 , and several glucose metabolic pathway genes . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
AIB 1 deficiency suppressed the up regulation of the insulin receptor substrate ( IRS ) 1 and IRS 2 and thereby inhibited the activation of Akt , expression of cyclin D 1 , and cell proliferation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Compared with 5 mM glucose , incubation of HAEC with 30 mM glucose for up to 48 h increased in a time dependent manner expression of insulin receptor , insulin receptor substrate ( IRS ) 1 , IRS 2 , and GLUT 1 proteins . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Inappropriate degradation of insulin signaling molecules such as insulin receptor substrates ( IRS 1 and IRS 2 ) has been demonstrated in experimental diabetes , mediated in part through the up regulation of suppressors of cytokine signaling ( SOCS ) . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Although insulin receptor protein level and phosphorylation were unaltered by resistin , production of IRS 1 , but not IRS 2 , was downregulated and a decreased tyrosine phosphorylation of IRS 1 was detected . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Polymorphic alleles of both IRS 1 and IRS 2 ( insulin receptor substrate 1 2 ) , alone or in combination , may have a functional impact on the insulin resistant component of PCOS . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Insulin receptor substrates , including Irs 1 and Irs 2 , integrate insulin and IGF receptor signals with heterologous pathways to coordinate growth and metabolism . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The following parameters were examined : morphological analysis of endocrine pancreata by immunohistochemistry ; protein levels of insulin receptor , IRS 1 , IRS 2 , PI 3 kinase , Akt 1 , and Akt 2 ; and static insulin secretion in isolated pancreatic islets . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IRS 1 and IRS 2 are the major insulin receptor substrates leading to glucose homeostasis , and have distinct and overlapping roles in diverse organs . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In these cells , the type 1 insulin like growth factor ( IGF 1 ) and granulocytic colony stimulating factor ( G CSF ) induce differentiation to granulocytes . 32D cells do not express insulin receptor substrate 1 ( IRS 1 ) or IRS 2 , docking proteins of the IGF 1 receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Activation of insulin receptor substrates 1 and 2 ( IRS 1 / IRS 2 ) was studied in rat and human myoblasts and rat cardiomyocytes . ^^^ Glulisine and RHI showed similar insulin receptor mediated phosphorylation and IRS 2 activation . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
After 12 weeks of diabetes , constitutive insulin receptor autophosphorylation and IRS 2 expression were reduced , without changes in p42 / p44 mitogen activated protein kinase or IRS 1 . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
While FRS 3 over expression in PC 12 cells neither increases NGF induced neuritogenesis nor activation of Map kinase / AKT , comparable to previous reports on FRS 2 , over expression of a chimeric adapter containing the PH / PTB domains of the insulin receptor substrate ( IRS ) 2 , in place of the PTB domain of FRS 3 ( IRS 2 FRS3 ) supports insulin dependent Map kinase activation and neurite outgrowth in PC 12 cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
IRS 2 phosphorylation induced by both insulin and AspB 10 insulin was nearly insensitive to blocking the insulin receptor , being predominantly mediated by the IGF 1 receptor . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
Nicotine produced time ( > 12 h ) and concentration ( EC ( 50 ) 3 . 6 and 13 microm ) dependent increases in insulin receptor substrate ( IRS ) 1 and IRS 2 levels by approximately 125 and 105 % , without altering cell surface density of insulin receptors . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
There were increased associations of insulin receptor substrate ( IRS ) 1 and IRS 2 with phosphatidylinositol ( PI ) 3 kinase , followed by enhanced protein kinase B ( Akt ) serine and threonine phosphorylation , in the ovaries of rats that were treated with hCG , either alone or with insulin . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
In the present study , we investigated the protein levels and phosphorylation status of the insulin receptor and insulin receptor substrates ( IRS 1 , IRS 2 , and IRS 3 ) as well as their association with PI ( 3 ) kinase in the rat adipose tissue of two models of insulin resistance : dexamethasone treatment and aging . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
The detection of mRNAs for insulin receptor ( IR ) A and IRB ; insulin receptor substrate ( IRS ) 1 and IRS 2 ; phosphoinositide 3 kinase ( PI3K ) catalytic subunits p110alpha , p110beta , PI3KC2alpha , and PI3KC2gamma ; phosphoinositide dependent protein kinase 1 ; protein kinase B ( PKB ) alpha , PKBbeta , and PKBgamma in the beta cell population suggests the presence of a functional insulin signaling cascade in human beta cells . ^^^
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9Y4H2 and P06213	Pubmed	SVM Score :0.0
NA