Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
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Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.53923479
The Tyr ( P ) forms of the insulin receptor and its 160 kDa substrate protein ( pp 160 ) associated with fusion proteins containing either or both the SH 2 domains of PI3K , but not with fusion proteins containing the two SH 2 domains of GAP or phospholipase C gamma . 0.53923479^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.65226269
The p 85 alpha regulatory subunit of PtdIns 3 ' kinase co precipitated with activated insulin receptor as revealed by immunoblotting . 0.65226269^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.5169982
In response to IGF 1 , insulin receptor substrate 1 was tyrosine phosphorylated and formed a complex with the PI3K heterodimer that consists of a p 85 regulatory subunit and a p 110 catalytic subunit . 0.5169982^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.61498238
Activation of PI3K by glucose dependent insulinotropic polypeptide and glucose was associated with insulin receptor substrate isoforms insulin receptor substrate 2 and growth factor bound 2 associated binder 1 and PI3K isoforms p85alpha , p110alpha , p110beta , and p110gamma . 0.61498238^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.6000195
Although , IGF 1 stimulated PI3K activity associated with insulin receptor substrates was unaltered in all cell lines , p85alpha null ES cells showed diminished protein kinase B activation despite increased PI3K activity associated with the p85beta subunit . 0.6000195^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Sam is a substrate of the insulin receptor and associates with the SH 2 domains of p 85 PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
PMA also decreased IGF 1 induced tyrosine phosphorylation of insulin receptor substrate 1 and its association with PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
PI3K activation was inhibited by wortmannin and was not dependent upon insulin receptor substrate 1 activation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin produced minimal activation of insulin receptor substrate ( IRS ) 1 associated PI3K activity in fetal liver . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin and naringenin increased HepG 2 cell PI3K activity and decreased insulin receptor substrate ( IRS ) 2 levels . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The major cytosolic substrate of the insulin receptor is a 185 kDa phosphoprotein ( IRS 1 ) that contains multiple putative attachment sites for the p 85 alpha regulatory subunit of phosphatidylinositol 3 ' kinase ( PI3K ) . ^^^ As p 85 alpha was not detectably phosphorylated on tyrosine residues and did not appear to interact directly with the insulin receptor , we conclude that tyrosine phosphorylation of pp 185 promotes its association with p 85 alpha and the catalytic subunit of PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin receptor signaling stimulated the association of IRS 1 with p 85 alpha protein , and p 85 alpha associated PI 3 kinase activity in 3T3 L 1 adipocytes , and in transfected Chinese hamster ovary cells ( CHO T ) and COS 1 cells expressing high levels of human insulin receptors . ^^^ Remarkably , the large stimulation of PI 3 kinase activity associated with p 85 alpha ( 7 . 8 + / 2 . 0 fold , n = 6 ) in insulin treated CHO T cells was not observed in p 85 beta immunoprecipitates ( 1 . 8 + / 0 . 6 fold , n = 6 ) , and in COS 1 cells p 85 beta associated PI 3 kinase activity was completely insensitive to stimulation by the insulin receptor . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
LY 294002 had no effect on tyrosine phosphorylation of both the insulin receptor beta subunit and insulin receptor substrate 1 ( IRS 1 ) and did not influence the association of the p 85 subunit of PI3K with IRS 1 . ^^^ These data suggest that : 1 ) PI3K plays a crucial role in various functions of insulin ; and 2 ) there exist multiple signaling pathways ( both PI3K dependent and PI3K independent ) for the insulin receptor . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We investigated whether phosphatidylinositol 3 kinase ( PI3K ) is phosphorylated and whether its specific activity is increased by insulin stimulation in vivo using Fao cells and antibodies raised against the 85 kDa subunit of PI3K , insulin receptor substrate 1 ( IRS 1 ) , and phosphotyrosine ( pTyr ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Both the antiPTyr PI3K stimulation and activation of insulin receptor tyrosine kinase were dependent on hormone concentration . ^^^ The results demonstrate that : ( a ) antiPTyr PI3K activity is responsive to insulin in mouse skeletal muscle , ( b ) both the insulin responsiveness and sensitivity of this activity are blunted in insulin resistant muscles from obese mice , ( c ) these alterations result from a combination of insulin receptor and postreceptor defects , and ( d ) starvation restores normal insulin responses . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We have identified two novel alternatively spliced forms of the p85alpha regulatory subunit of phosphatidylinositol ( PI ) 3 kinase by expression screening of a human skeletal muscle library with phosphorylated baculovirus produced human insulin receptor substrate 1 . ^^^ Both p 85 and p85 / AS53 bind to p 110 in coprecipitation experiments , but p85alpha itself appears to have preferential binding to insulin receptor substrate 1 following insulin stimulation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Using reverse transcription competitive PCR , we have measured the mRNAs encoding the two insulin receptor variants , the insulin receptor substrate 1 , the p85alpha subunit of phosphatidylinositol 3 kinase , Ras associated to diabetes ( Rad ) , the glucose transporter Glut 4 , glycogen synthase , 6 phosphofructo l kinase , lipoprotein lipase , and the hormone sensitive lipase . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The objective of this study was to investigate the mechanism of dexamethasone action by determining its effects on the expression of the p85alpha and p85beta regulatory subunit isoforms of PI 3 kinase , their coupling with the p 110 catalytic subunit , and their association with insulin receptor substrate 1 ( IRS 1 ) in response to IGF 1 stimulation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Moreover , we studied the insulin mediated signal transduction pathways starting from insulin receptor , such as the Src kinase , the p21Ras / ERK , and the PI3K routes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
No difference in mRNA expression between omental and subcutaneous adipose tissue was observed for hormone sensitive lipase , lipoprotein lipase , 6 phosphofructo 1 kinase , insulin receptor substrate 1 , p85alpha regulatory subunit of phosphatidylinositol 3 kinase , and Rad . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Genetic and biochemical studies indicated that this impairment was not secondary to a defect in the structure , expression , or activation of the insulin receptor , IRS 1 , or p85alpha . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Cytochalasin D also decreased significantly the insulin dependent association of PI 3 kinase activity and the levels of insulin receptor substrate ( IRS ) 1 , p85alpha and p110beta with immunopurified GLUT 4 containing compartments . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In rat HTC hepatoma cells overexpressing normal human insulin receptors ( HTC IR ) , p 85 regulatory subunit of phosphatidylinositol 3 kinase ( PI3K ) forms signaling complexes containing the insulin receptor , insulin receptor substrate 1 ( IRS 1 ) , guanosine triphosphatase activating protein ( GAP ) and 60 70 kDa phosphotyrosine proteins ( p 60 70 ) . ^^^ These data suggest that 1 ) GAP associated protein , but not Sam 68 , is a part of insulin signaling complexes ; and 2 ) p85alpha and p85beta form similar , but distinct , insulin receptor signaling complexes . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Ligand activated tyrosine kinase receptors of insulin superfamily peptides can realize the signal transduction to SH 2 proteins ( phosphatidylinositol 3 kinase , PI3K ) , protein phosphotyrosine phosphatase ( PPTP ) , GRB 2 adaptor protein in two pathways : 1 ) with participation of specific proteins insulin receptor substrates 1 and 2 ( IRS1 / IRS2 ) ; and 2 ) direct interaction between receptors and SH 2 proteins ( without IRS proteins ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Recent data demonstrate that receptor positive neurons in the brain , i . e . in the hypothalamus , and secretory cells in the anterior pituitary gland possess specific proteins that are thought to be involved in key steps of post receptor signal transduction , in particular insulin receptor substrate 1 and phosphatidylinositol 3 ' kinase ( PI3k ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
CR did not alter insulin ' s stimulation of insulin receptor substrate ( IRS ) 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin stimulated PI3K activity associated with insulin receptor substrates ( IRSs ) was mediated via full length p 85 alpha in wild type mice , but via the p 50 alpha alternative splicing isoform of the same gene in Pik3r1 / mice . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Cardiac insulin receptor and the p 85 alpha subunit of PI 3 kinase both declined by about 40 % , whereas the skeletal muscle content of these two proteins were unaffected by aging . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Recently , it has been reported that insulin induced glucose uptake is mediated by activation of second messengers such as insulin receptor substrate 1 ( IRS 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , and diacylglycerol ( DG ) protein kinase C ( PKC ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In skeletal muscle , insulin stimulated tyrosine phosphorylation was decreased in pregnant + / + mice , and even more so in db / + mice : insulin receptor beta ( IR beta ) , + / + 34 % , db / + 57 % decrease , P < 0 . 05 ; insulin receptor substrate 1 ( IRS 1 ) , + / + 44 % , db / + 61 % decrease , P < 0 . 05 ; and phosphoinositol ( PI ) 3 kinase ( p85alpha ) , + / + 33 % , db / + 65 % decrease , P < 0 . 05 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Inactivation of the insulin receptor homolog DAF 2 , the AGE 1 PI3K , or the AKT 1 and AKT 2 kinases causes a developmental arrest at the dauer stage . ^^^ Therefore , pdk 1 activity is both necessary and sufficient to propagate AGE 1 PI3K signals in the DAF 2 insulin receptor like signaling pathway . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In addition to requirements for PI3K , PDK 1 , and PKC zeta , we found that a tyrosine kinase ( presumably the insulin receptor ) , the SH 2 domain of GRB 2 , SOS , RAS , RAF , and MEK 1 were required for insulin effects on ERK in the rat adipocyte . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The variant did not affect the binding of IRS 2 to the insulin receptor or p85alpha of phosphatidylinositol 3 kinase when measured in the yeast two hybrid system . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The PI3K inhibitor LY 294002 completely blocks insulin stimulated glycogen synthesis by inhibiting glycogen synthase , PKB ( Akt 1 ) , and FRAP ( RAFT ) autophosphorylation , as well as p 70 S6 kinase activation , whereas insulin receptor substrates tyrosine phosphorylation and MEK activity were not affected . ^^^ However , the inhibition of FRAP and S 6 kinase activation is not sufficient to block insulin stimulated glycogen synthesis , suggesting an important role of a branching pathway upstream of S 6 kinase and downstream of PI3K , which is probably mediated by PKB in the signaling of the insulin receptor in hepatoma HTC cells . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Since many receptors that activate PI3K do so through tyrosine specific protein phosphorylation , antisera to phosphotyrosine , insulin receptor substrate 1 ( IRS 1 ) , or SPAR were also examined . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Compared with control cells , cells expressing high levels of PTP1B showed a 50 60 % decrease in maximally insulin stimulated tyrosyl phosphorylation of IR and insulin receptor substrate 1 ( IRS 1 ) and phosphoinositide 3 kinase ( PI3K ) activity associated with IRS 1 or with phosphotyrosine . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
This pathway involves stimulation of phosphatidylinositol 3 kinase ( PI3K ) binding to insulin receptor substrate 1 and insulin receptor substrate 2 , activation of PI3K and protein kinase B ( AKT ) , and PI3K dependent activation of cyclic nucleotide phosphodiesterase 3B , a cAMP degrading enzyme . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In control subjects , the diet increased p 85 alpha PI3K ( +146 % ) , insulin receptor ( +100 % ) and Rad ( +40 % ) mRNA concentrations in muscle . ^^^ In Type 2 diabetic patients , the diet increased insulin receptor ( +41 % ) and Rad ( +31 % ) mRNAs but the expression of p 85 alpha PI3K was not modified . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In control ( not insulin treated ) rats , there was no detectable tyrosine phosphorylation of insulin receptor ( IR ) , regardless of diet ; no diet effect on tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) or IRS 1 associated phosphatidylinositol 3 kinase ( PI3K ) protein and 21 % higher IRS 1 associated PI3K activity in AL vs . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Employing antibodies to insulin receptor substrate 3 ( alpha IRS 3 ) , we found that IRS 3 is a major p 60 phosphoprotein that is tyrosine phosphorylated following insulin treatment of cells and interacts with phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Phosphoinositide 3 kinase ( PI3K ) activity associated with insulin receptor substrate ( IRS ) 1 or phosphotyrosine was reduced 67 86 % in tissues of obese rats because of lower IRS 1 protein levels and reduced insulin receptor and IRS 1 phosphorylation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Dexamethasone induces overexpression of the PI3K subunit p85alpha , which , in turn , competes with the complete PI3K heterodimer for binding at insulin receptor substrate 1 , inhibiting PI3K activation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Tyrosine phosphorylation of the activated insulin receptor , insulin receptor substrate ( IRS ) 1 , and p 60 70 was also blunted , preventing their association with p 85 phosphatidylinositol 3 kinase ( PI3K ) and their activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Here we investigate the requirement of MAP ( MAPK ) and phosphatidylinositol 3 ( PI3K ) kinases in the transformation of rat intestinal epithelial ( RIE ) cells by oncogenic forms of insulin receptor ( gag IR ) , insulin like growth factor 1 receptor ( gag IGFR ) , and 5 Src . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Essentially no p 85 alpha subunit of PI 3 kinase was found to be associated with insulin receptor substrate ( IRS ) 1 or IRS 2 in basal and insulin stimulated ( 5 min ) cardiomyocytes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
This diminished ability of insulin to stimulate glucose transport was accompanied by a reduction in the total cellular content of insulin receptor substrates 1 and 2 and the p85alpha subunit of phosphatidylinositol 3 ' kinase . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The gene expression of insulin receptor substrate 2 and the p 85 alpha subunit of phosphatidylinositol 3 kinase was significantly higher 3 h after a single exercise bout , although short term training ameliorated this effect . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Immunoprecipitation revealed that a PI 3 kinase regulatory subunit , p85alpha , was associated with insulin receptor substance 1 ( IRS 1 ) phosphorylated by H2O2 treatment . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Upon ligand binding , the IGF 1 receptor phosphorylates tyrosine residues in SHC and insulin receptor substrates ( IRSs ) initiating two main signalling cascades , the MAP kinase and the phosphatidylinositol 3 kinase ( PI3K ) pathways . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The purpose of this study was to investigate the possibility that PKC delta may be activated upstream of PI3K by direct interaction with insulin receptor ( IR ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The up regulation of Id 2 gene expression was largely dependent on the presence of insulin receptor substrate 1 , a major substrate of the IGF IR and a potent activator of the phosphatidylinositol 3 kinase ( PI3K ) pathway . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
By transient transfecion of 293 cells , we show that p110alpha hybrids , although unable to support lipid dependent PI3K signaling , such as activation of protein kinase B / Akt and p 70 ( S6k ) , retain the capability to associate with and phosphorylate insulin receptor substrate 1 , with the same specificity and higher efficacy than wild type PI3Kalpha . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Basal mRNA levels ( determined by reverse transcriptase competitive polymerase chain reaction ) of insulin receptor , insulin receptor substrate 1 , p85alpha phosphatidylinositol 3 kinase ( PI3K ) , p110alphaPI3K , p110betaPI3K , GLUT 4 , glycogen synthase , and sterol regulatory element binding protein 1c ( SREBP 1c ) were similar in muscle of control ( n = 17 ) , type 2 diabetic ( n = 9 ) , type 1 diabetic ( n = 9 ) , and nondiabetic obese ( n = 9 ) subjects . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
METHODS : The effect of BRL 49653 ( Rosiglitazone ) on the mRNA expression of insulin receptor , insulin receptor substrate 1 , p85alpha , p110alpha and p110beta subunits of phosphatidylinositol 3 kinase , Glut 4 and hormone sensitive lipase was examined in isolated adipocytes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In a set of in vivo experiments , the levels of alpha subunit of the insulin receptor , the insulin receptor substrate 1 ( IRS 1 ) and the phosphatidylinositol 3 kinase ( PI3K ) were determined . [ 3H ] thymidine incorporation into DNA 24 or 48 h after surgery was assessed in all the experimental groups . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
After IGF 1 stimulation , insulin receptor substrate 1 and 2 phosphorylation and PI3K activity were restored to levels similar to or greater than those seen in wild type cells . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Whereas insulin gene transcription is promoted by signaling through insulin receptor A type ( Ex 11 ) , PI3K class Ia , and p70s6k , insulin stimulates the betaGK gene by signaling via insulin receptor B type ( Ex11+ ) , PI3K class 2 like activity , and PKB ( c Akt ) . ^^^ Our data provide evidence for selectivity in insulin action via the two isoforms of the insulin receptor , the molecular basis being preferential signaling through different PI3K and protein kinases . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Furthermore , E 2 induced diminution of uterine insulin receptor substrate 2 protein was only partially inhibited in the presence of wortmannin , a PI3K inhibitor . ^^^ Collectively , these data suggest that the E 2 induced decrease in uterine insulin receptor substrate 2 requires IGF 1 signaling , is not dependent solely on insulin receptor substrate 1 and PI3K , and is blocked by progesterone as well as by pharmacological inhibition of proteasomal protease activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Moreover , analysis of the levels and subcellular distribution of insulin receptor substrates 1 and 2 , the p85alpha subunit of phosphatidylinositol 3 ' kinase , and protein kinase B ( PKB ) / cAKT in skeletal muscle did not identify any differences between the two strains ; the insulin dependent activation of PKB / cAKT was not different between the two strains . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Evidence has suggested that the insulin receptor substarate ( IRS ) phosphoinositide 3 kinase ( PI3K ) pathway plays a central role in metabolic actions of insulin . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
By activating the Insulin receptor ( IR ) > IRS 1 > phosphatidylinositol 3 kinase ( PI3K ) > Akt pathway , insulin protects vascular smooth muscle cells ( VSMC ) from undergoing apoptosis . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Muscle levels of insulin receptor ( IR ) , insulin receptor substrate 1 ( IRS 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , and Akt 1 , a serine threonine kinase , were determined . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Sam 68 is a docking protein linking GAP and PI3K in insulin receptor signaling . ^^^ We have recently found that Sam 68 is a substrate of the insulin receptor ( IR ) and that Tyr phosphorylated Sam 68 associates with the SH 2 domains of p 85 PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The mRNA levels of insulin receptor , p 85 alpha phosphatidylinositol 3 kinase ( p 85 alpha PI 3K ) , insulin dependent glucose transporter ( Glut 4 ) , hexokinase 2 , glycogen synthase , lipoprotein lipase ( LPL ) in muscle and in adipose tissue , hormone sensitive lipase and peroxisome proliferator activated receptor gamma ( PPAR gamma ) in adipose tissue were quantified by RT competitive PCR . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Expression and phosphorylation of the cytoplasmic tail of retina insulin receptor showed direct involvement with the p 85 subunit of PI3K in vitro . ^^^ CONCLUSIONS : Tyrosine phosphorylation of the beta subunit of the insulin receptor is involved in the regulation of PI3K activity in ROS . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
These results indicate that in addition to their roles in recruiting the catalytic subunit of PI3K to the insulin receptor substrate proteins , both p85alpha and p85beta play negative roles in insulin signaling . . ^^^ In addition , insulin dependent tyrosine phosphorylation of insulin receptor substrate 2 was enhanced in the p85beta ( / ) mice , a phenotype not observed in the p85alpha ( / ) mice . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
They had phosphorylation of the IGF 1 receptor beta subunit , phosphorylation of insulin receptor substrate ( IRS ) 1 , and association of the p 85 subunit ( phosphatidylinositol 3 kinase [ PI3K ] ) with the IGF 1 receptor and IRS 1 in D NOD cells in the basal state . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Similar to TNF , cycloheximide and anisomycin stimulated serine phosphorylation of IRS 1 and IRS 2 , reduced their ability to interact with the insulin receptor , inhibited the insulin induced tyrosine phosphorylation of IRS proteins , and diminished their association with phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Heterozygous disruption of Pik3r1 improves insulin signaling and glucose homeostasis in normal mice and mice made insulin resistant by heterozygous deletion of the Insulin receptor and / or insulin receptor substrate 1 ( IRS 1 ) genes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin stimulation of insulin receptor substrate ( IRS ) 1 , IRS 2 , and antiphosphotyrosine associated phosphatidylinositol 3 kinase ( PI3K ) was similar for CR and AL . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We sought to examine the effects of elevated plasma NEFA ( approximately 1 mmol / liter ) on muscle PI3K activity , insulin receptor substrate ( IRS ) 1 ( important for activation of PI3K ) , and Akt , which is downstream of PI3K and activated by phosphorylation on serine and threonine in a PI3K dependent manner . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Adipose tissue insulin receptor substrate ( IRS ) 1 protein levels were 43 % lower ( P = 0 . 02 ) and p85alpha subunit of phosphatidylinositol 3 kinase was twofold higher ( P = 0 . 03 ) in GDM compared with Preg Con subjects . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Both p85alpha ( Ile / Asp ) and p85alpha ( Ile / Asn ) also exhibited increased binding to phospho insulin receptor substrate 1 by 41 % and 83 % , respectively ( P < 0 . 001 ) , as compared with p85alpha ( Met / Asp ) . ^^^ The expression of p85alpha ( Ile ) was also slightly decreased and the binding to insulin receptor substrate 1 slightly increased in brown preadipocytes derived from p85alpha knockout mice . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Immunoprecipitation of both receptor forms by antibodies against the alpha subunit and against the carboxy terminus of the beta subunit of the IGF IR suggests that the 75 kDa form could be the beta chain truncated at the amino terminus above the alpha beta disulphide bridges . 3 ) The ATA activated IGF IR forms underwent slow dephosphorylation , compared with a rapid dephosphorylation of the IGF 1 activated receptor . 4 ) The insulin receptor substrate 1 / 2 associated PI3K , Shc proteins , and the kinases Akt and Erk1 / 2 , downstream mediators of the antiapoptotic signaling by IGF IR , were activated to a higher extent and for a longer time period by ATA , compared with IGF 1 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Although plasma insulin had reached 98 % of its final concentration within 10 min , insulin receptor kinase ( IRK ) activity , p 85 associated with insulin receptor substrate 1 ( IRS 1 ) , IRS 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity , and Thr ( 308 ) protein kinase B ( PKB ) phosphorylation in the muscle biopsies at 20 min had reached only 60 , 48 , 34 and 47 % respectively of those at 180 min . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The appearance of a complex between tyrosine phosphorylated insulin receptor substrate 1 ( IRS 1 ) and PI3K in a high speed pellet fraction ( HSP ) is thought to be a key event in insulin action . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In Drosophila , components of the insulin receptor phosphoinositide 3 kinase ( PI3K ) pathway determine body , organ , and cell size . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
It was found that IGF 1 induced phosphatidylinositol 3 kinase ( PI3K ) association with insulin receptor substrate ( IRS ) 1 and 2 in a glucose dependent manner , whereas TGF alpha / EGF did not . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Our results suggest that in 3T3 L 1 adipocytes , shikonin action is not mediated primarily via the insulin receptor / PI3K pathway , but rather via another distinct tyrosine kinase dependent pathway leading to glucose uptake involving Akt phosphorylation . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Presently , we found that insulin signaling to insulin receptor substrate 1 dependent PI3K , PKB , and PKC lambda , and downstream processes , glucose transport and activation of ERK , were enhanced in skeletal muscles and adipocytes of mice in which the ubiquitous conventional diacylglycerol sensitive PKC isoform , PKC alpha , was knocked out by homologous recombination . ^^^ We conclude that 1 ) PKC alpha is not required for insulin stimulated glucose transport , and 2 ) PKC alpha is activated by insulin at least partly independently of PI3K , and largely serves as a physiological feedback inhibitor of insulin signaling to the insulin receptor substrate 1 / PI3K / PKB / PKC lambda / zeta / iota complex and dependent metabolic processes . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The thiazolidenedione , rosiglitazone , increases basal and / or insulin stimulated glucose transport in various cell types by diverse but uncertain mechanisms that may involve insulin receptor substrate ( IRS ) 1 dependent PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We have recently found that Sam 68 is a substrate of the insulin receptor ( IR ) that translocates from the nucleus to the cytoplasm and that Tyr phosphorylated Sam 68 associates with the SH 2 domains of p 85 PI3K and GAP , in vivo and in vitro . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In addition , an increase in insulin receptor substrate 2 protein and a differential regulation of the phosphatidylinositol 3 kinase regulatory subunit ( p85alpha ) isoforms expression were found in fat from antisense treated animals , although increased insulin sensitivity measured by protein kinase B phosphorylation was not observed . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin signaling proteins , insulin receptor substrate 2 and phosphatidylinositol 3 ( PI 3 ) kinase regulatory subunit p50alpha , were increased and PI 3 kinase p85alpha expression was decreased in liver and fat . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Western blot analyses revealed the presence of the insulin receptor , insulin receptor substrate proteins 1 and 2 and PI3K in CD 1 islets . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In the biopsies , insulin receptor kinase ( IRK ) activity , insulin receptor substrate ( IRS ) 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity , Ser ( 473 ) and Thr ( 308 ) phosphorylation of protein kinase B ( PKB ) , and protein expression of IRS 1 , IRS 2 , phosphoinositol dependent kinase 1 ( PDK 1 ) , PKB , and GLUT 4 were determined . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In the present study , we identified novel negative cross talk between the angiotensin 2 subtype 2 ( AT 2 ) receptor and insulin receptor signaling in the regulation of phosphoinositide 3 kinase ( PI3K ) , Akt , and apoptosis in rat pheochromocytoma cell line , PC12W cells , which exclusively express AT 2 receptor . ^^^ We demonstrated that insulin mediated insulin receptor substrate ( IRS ) 2 associated PI3K activity was inhibited by AT 2 receptor stimulation , whereas IRS 1 associated PI3K activity was not significantly influenced . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Recently , we have shown that phosphoinositide 3 kinase ( PI3K ) in bovine rod outer segment ( ROS ) is activated in vitro by tyrosine phosphorylation of the C terminal tail of the insulin receptor ( Rajala , R . 5 . ^^^ In this study , we have investigated the in vivo mechanism of PI3K activation in the rodent retina and report the novel finding that light stimulates tyrosine phosphorylation of the beta subunit of the insulin receptor ( IRbeta ) in ROS membranes , which leads to the association of PI3K enzyme activity with IRbeta . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Genetic screens that identified many components of the C . elegans insulin pathway did not identify homologs of insulin receptor substrates or the phosphoinositide 3 kinase ( PI3K ) adaptor / regulatory subunit , which are both required for signaling by mammalian insulin / insulin like growth factor 1 pathways . ^^^ The C . elegans versions of insulin receptor substrate ( IST 1 ) and PI3K p50 / p55 ( AAP 1 ) share moderate sequence similarity with their vertebrate and Drosophila counterparts . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Here we report that SP A treatment leads to rapid tyrosine specific phosphorylation of several important proteins in lung epithelial cells including insulin receptor substrate 1 ( IRS 1 ) , an upstream activator of PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We investigated the effect of ceramide on insulin signaling pathways , such as insulin receptor ( IR ) beta subunit , insulin receptor substrate 1 ( IRS 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , and protein kinase Czeta ( PKCzeta ) in rat adipocytes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In peripheral tissues , insulin signaling involves activation of the insulin receptor substrate ( IRS ) phosphatidylinositol 3 kinase ( PI3K ) enzyme system . ^^^ Here , we report that insulin induces tyrosine phosphorylation of the insulin receptor and IRS 1 and 2 , increases binding of activated IRS 1 and 2 to the regulatory subunit of PI3K , and activates protein kinase B / Akt , a downstream target of PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The MEK 1 mutant , which activates ERK , markedly down regulated expression of the insulin receptor ( IR ) and its major substrates , IRS 1 and IRS 2 , mRNA and protein , and in turn reduced tyrosine phosphorylation of IR as well as IRS 1 and IRS 2 and their associated phosphatidyl inositol 3 kinase ( PI3K ) activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Tyrosine phosphorylation of insulin receptor substrate 1 is detected when enriched by immunoprecipitation with anti PI3K antibody . ^^^ In summary , selenium stimulates glucose transport and antilipolysis in a PI3K dependent manner , but independent of insulin receptor activation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Desensitization was correlated to a reduction in insulin receptor substrate ( IRS ) 1 and IRS 2 protein levels , which was reversed by the PI3K inhibitor LY 294002 . ^^^ In summary , ( 1 ) PI3K mediates insulin induced reduction of IRS 1 by phosphorylating it while a PI3K / mTOR pathway controls insulin induced reduction of IRS 2 , ( 2 ) in L 6 cells , IRS 2 is the major adapter molecule linking the insulin receptor to activation of PKB and MAPK , ( 3 ) the mechanism of IRS 1 / 2 down regulation is different in L 6 cells compared with 3T3 L 1 adipocytes . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Exposure to 100 nM IGF 1 for 10 min resulted in increased insulin receptor substrate 1 associated PI3K activity and Akt kinase activity , whereas 100 nM Ang 2 pretreatment for 5 min strikingly decreased these IGF 1 effects . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin receptor substrate ( IRS ) 1 was associated with PI3K activity , which is necessary for Akt activation by insulin , and was reduced in obese muscles , and this was accompanied by decreased IRS 1 expression . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Exposure to IGF 1 for 30 minutes or E 2 for 20 minutes increased insulin receptor substrate 1 ( IRS 1 ) association with the regulatory ( p 85 ) subunit of PI3K , enhanced tyrosine phosphorylation of p 85 , and increased PI3K activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Although naringenin did not alter the phosphotyrosine status of the insulin receptor , insulin receptor substrate proteins , or PI3K , it did inhibit the phosphorylation of the downstream signaling molecule Akt . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In addition , stimulation of the phosphatidylinositol 3 kinase ( PI3K ) pathway through recruitment of members of the insulin receptor substrate family may contribute to survival and proliferation . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin is a potent inducer of adipogenesis , and differentiation of adipocytes requires many components of the insulin signaling pathway , including the insulin receptor substrate IRS 1 and phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin stimulated insulin receptor substrate ( IRS ) 1 tyrosine phosphorylation and PI3K activity are impaired 40 50 % in diabetic subjects compared with lean or obese subjects . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Immunoprecipitation / Western blot studies revealed that CLA inhibited IGF 1 induced phosphorylation of IGF IR and insulin receptor substrate ( IRS ) 1 , recruitment of the p 85 regulatory subunit of phosphatidylinositol 3 kinase ( PI3K ) to IGF IR , IGF IR associated PI3K activity , and phosphorylated Akt and extracellular signal regulated kinase ( ERK ) 1 / 2 levels . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
METHODS : We determined the expression and tyrosyl phosphorylation of the insulin receptor beta subunit ( IRbeta ) , IRS 1 and IRS 2 , the binding of phosphatidylinositol 3 kinase ( PI3K ) , and activation of the mitogen activated protein kinase ( MAPK ) pathway in the presence or absence of insulin stimulation in vivo during development and in the adult liver . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
PIP4K 2 beta expression did not impair insulin dependent association of PI3K with insulin receptor substrate 1 ( IRS 1 ) but abbreviated Akt activation , indicating that PIP4K 2 regulates PI 3 , 4 , 5 P 3 degradation rather than synthesis . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Furthermore , PDGF stimulates these effects independent of insulin receptor substrate ( IRS ) 1 or IRS 2 tyrosine phosphorylation or docking to activated PI3K . ^^^ These data demonstrate that 1 ) the basis of insulin specific glucose transport in cultured adipocytes is the low level of receptors for other growth factors and 2 ) in the presence of adequate receptors , PDGF is fully capable of activating glucose transport in a manner requiring PI3K and subsequent phosphatidylinositol 3 , 4 , 5 trisphosphate accumulation but independent of insulin , insulin receptor , and IRS proteins . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
This article , describes recent studies that link the photobleaching of rhodopsin to tyrosine phosphorylation of the insulin receptor and subsequent activation of phosphoinositide 3 kinase ( PI3K ) . ^^^ We recently found a light dependent increase in tyrosine phosphorylation of the insulin receptor beta subunit ( IR beta ) and an increase in PI3K enzyme activity in isolated rod outer segments ( ROS ) and in anti phosphotyrosine ( PY ) and anti IR beta immunoprecipitates of retinal homogenates . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Down regulation of insulin receptor tyrosine phosphorylation and subsequent steps in the insulin signalling pathway , including insulin receptor substrate 1 ( IRS 1 ) associated phosphoinositide 3 kinase ( PI3K ) , Akt kinase serine phosphorylation and activity and glucose transporter ( GLUT 4 ) protein content , are evident in skeletal muscle after eccentric exercise . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We could not confirm that a Val985Met variant in the insulin receptor is associated with type 2 diabetes or that the Met326Val of the p 85 alpha regulatory subunit of the phosphoinositide 3 kinase is associated with insulin resistance . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Pull down experiments with glutathione S transferase fused to the N Src homology 2 domain of p 85 , the regulatory subunit of PI3K , indicated that betaE 2 and alphaE 2 , but not progesterone , identified phosphorylated insulin receptor beta subunit ( IRbeta ) as a binding partner . ^^^ Pretreatment with insulin receptor inhibitor , HNMPA , inhibited IRbeta activation of PI3K . ^^^ The results show that estrogen has retinal neuroprotective properties in vivo and in vitro and suggest that the insulin receptor / PI3K / Akt signaling pathway is involved in estrogen mediated retinal neuroprotection . . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Leptin treatment at this dose improved insulin stimulated insulin receptor and insulin receptor substrate 2 ( IRS 2 ) phosphorylation , IRS 2 associated PI3K activity , and Akt activity in liver . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We also show that Src directed activation of insulin receptor substrate 1 , phosphatidylinositol 3 kinase ( PI3K ) , Akt , and Bad phosphorylation conform a substantial component of the survival route because pharmacological inhibition of PI3K and Akt canceled the antiapoptotic effects of NO . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
OBJECTIVE : Insulin responsive adipogenic signaling molecules include insulin receptor substrates ( IRS ) 1 and 2 , phosphoinositide 3 kinase ( PI3K ) , and protein kinase B ( PKB ; also known as Akt ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Three lines of evidence indicate that the increase in CD 36 is caused by defective insulin signaling : ( a ) Treatment of wild type macrophages with LY 294002 , an inhibitor of insulin signaling via PI3K , results in an increase in CD 36 ; ( b ) insulin receptor knockout macrophages show a post transcriptional increase in CD 36 protein ; and ( c ) administration of thiazolidinediones to intact ob / ob mice and ob / ob , LDL receptor deficient mice results in a reversal of macrophage insulin receptor defects and decreases CD 36 protein . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Conversely , chemicals known to inhibit the mammalian insulin receptor or downstream elements of its signaling pathway , such as LY 294002 , an inhibitor of phosphatidylinositol 3 kinase ( PI3K ) , were able to prevent the steroidogenic action of bovine insulin on fly ovaries . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The aim of our study was to investigate whether common polymorphisms in the genes regulating the early insulin signalling pathway ( insulin ; A 23T , insulin like growth factor 1 receptor [ IGF 1R ] ; GAG1013GAA , plasma cell membrane glycoprotein 1 [ PC 1 ] ; K121Q , insulin receptor substrate [ IRS 1 ] ; G972R , insulin receptor substrate 2 [ IRS 2 ] ; G1057D and phosphatidylinositol 3 kinase p 85 alpha [ PI3K ] ; M326I ) affect the weight change and development of Type 2 diabetes in the Finnish Diabetes Prevention Study . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Recently , we have shown that phosphoinositide 3 kinase ( PI3K ) in retina is regulated in vivo through light activation of the insulin receptor beta subunit . ^^^ In this study , we have cloned the 41 kDa cytoplasmic region of the retinal insulin receptor ( IRbeta ) and used the two hybrid assay of protein protein interaction in the yeast Saccharomyces cerevisiae to demonstrate the interaction between the p 85 subunit of PI3K and the cytoplasmic region of IRbeta . ^^^ Further , the Y960F mutant insulin receptor , which does not bind IRS 1 , is capable of bringing down PI3K activity from retina lysates . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
A recombinant IRS 1 fragment ( rIRS 1 ( 449 ) ( ) ( 664 ) ) containing major tyrosine motifs for interaction with phosphatidylinositol ( PI ) 3 kinase strongly associated to the p85alpha subunit of PI 3 kinase after Tyr phosphorylation by the insulin receptor . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin receptor substrate 1 ( IRS 1 ) associated PI3K activity was not altered in cells acidified for 10 min but was strongly inhibited in cells incubated at pH 7 . 1 for 24 h . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In these cells , AKT is activated by upstream molecules such as beta adrenergic receptor , insulin like growth factor 1 or insulin receptor , through PI3K alpha ; whereas its activation is inhibited by the PTEN molecule . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin receptor substrates ( IRSs ) 1 and 2 are postulated to control the activation of phosphatidylinositol 3 kinase ( PI3K ) dependent signaling factors , namely , atypical protein kinase C ( aPKC ) and protein kinase B ( PKB ) / Akt , which mediate metabolic effects of insulin . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Relative to adipocytes of lean women , insulin stimulated [ ( 3 ) H ] 2 deoxyglucose uptake and activation of insulin receptor substrate 1 / PI3K and aPKCs , but not PKB , were diminished in adipocytes of obese women . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Protein levels and phosphorylation of the insulin receptor and insulin receptor substrate 1 / 2 ( IRS 1 / 2 ) were measured , as was the association between IRS 1 / 2 and phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We hypothesized that PI3K and MAPK , the major pathways involved in insulin receptor signaling , mediate sympathetic nerve responses to insulin . ^^^ Ex vivo biochemical studies of mediobasal hypothalamic tissue revealed that insulin stimulated the association of insulin receptor substrate 1 with the p85alpha subunit of PI3K and also tyrosine phosphorylation of p 42 and p 44 subunits of MAPK in the hypothalamus . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
A 5 hour lipid infusion decreased insulin stimulated skeletal muscle glucose uptake in the WT mice that was associated with 40 50 % decreases in insulin stimulated tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) and IRS 1 associated PI3K activity . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
This is the result of downregulation of the insulin receptor substrates , IRS 1 and IRS 2 , which become limiting for signal transmission from the insulin receptor to PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
In the present study , we examined the protein levels and phosphorylation state of insulin receptor ( IR ) , IRS 1 and IRS 2 , the association between IRSs and PI3K and SHP 2 , the insulin induced IRSs associated PI 3 kinase activities , and the phosphorylation status of AKT and atypical PKCzeta / lambda in the liver and the muscle of 6 month old Wistar rats treated with DHEA . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Interestingly , those patients revealed an impaired insulin mediated insulin receptor substrate ( IRS ) 1 binding to p 85 alpha without any alteration in IRS 2 / p85 alpha association . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We show that IL 1beta decreases insulin induced tyrosine phosphorylation of the insulin receptor ( IR ) and insulin receptor substrate ( IRS ) proteins as well as phosphatidylinositol 3 kinase ( PI3K ) activation , and that this action is not due to the IL 1beta dependent nitric oxide ( NO ) production in RINm5F cells . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Although insulin receptor substrate 1 ( IRS 1 ) and IRS 2 , among other factors , activate PI3K , there is little information on the relative roles of IRS 1and IRS 2 during aPKC activation by insulin action in specific cell types . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We have recently demonstrated that PRL also stimulates the insulin receptor substrates / phosphatidylinositol 3 kinase ( IRSs / PI3K ) and SH 2 plekstrin homology domain ( SHC ) / ERK pathways in islets of neonatal rats . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The main pathway involved in insulin induction of adipogenic differentiation , monitored by fatty acid synthase expression , is the cascade insulin receptor substrate ( IRS ) 1 / phosphatidylinositol 3 kinase ( PI3K ) / Akt . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Because activation of PKB can require insulin receptor substrate proteins ( IRS 1 and IRS 2 ) and phosphatidylinositol 3 kinase ( PI3K ) , it is of interest to determine whether the activity of Foxo 1 is also regulated by heterotrimeric G protein coupled receptors ( GPCRs ) with IRS 1 or 2 , PI3K , or PKB signaling potential . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Here we show that in 3T3 L 1 adipocytes , resistin attenuates multiple effects of insulin , including insulin receptor ( IR ) phosphorylation , IR substrate 1 ( IRS 1 ) phosphorylation , phosphatidylinositol 3 kinase ( PI3K ) activation , phosphatidylinositol triphosphate production , and activation of protein kinase B / Akt . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We have recently found that homocysteine thiolactone inhibits insulin receptor tyrosine kinase activity , which results in decreased phosphatidylinositol 3 kinase ( PI3K ) activity and inhibition of glycogen synthesis . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We have also showed that insulin and leptin are able to activate PI3K through IRS 1 ( insulin receptor substrate 1 ) and IRS 2 respectively . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The signaling cascade initiated by IL 4 is classically divisible into two major pathways : one mediated by STAT 6 , and the other by insulin receptor substrates 1 and 2 via activation of PI3K . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
These defects in muscle aPKC activation are due to both impaired activation of insulin receptor substrate 1 dependent PI3K ( phosphoinositide 3 kinase ) and the direct activation of aPKCs by the lipid product of PI3K , PI 3 , 4 , 5 ( PO 4 ) 3 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Using pharmacological , biochemical , and cell biological approaches , we show that leptin induced platelet activation required activation of a signaling cascade that included the long form of the leptin receptor , three kinases [ Janus kinase 2 ( JAK 2 ) , phosphatidylinositol 3 kinase ( PI3K ) , and protein kinase B ( PKB / Akt ) ] , the insulin receptor substrate 1 ( IRS 1 ) , and the major human platelet cAMP phosphodiesterase phosphodiesterase 3A ( PDE3A ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Consequently , our results indicated that the cross talk between Akt 1 and JNK1 / 2 could be mediated by insulin receptor through PI3K in rat hippocampus during reperfusion . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Biochemical analyses demonstrated that chronic Akt activation induces feedback inhibition of PI3K activity through both proteasome dependent degradation of insulin receptor substrate 1 ( IRS 1 ) and inhibition of transcription of IRS 1 as well as that of IRS 2 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The APS adapter protein is recruited to the autophosphorylated kinase domain of the insulin receptor and initiates the phosphatidylinositol 3 kinase ( PI3K ) independent pathway of insulin stimulated glucose transport by recruiting CAP and c Cbl . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Physical association between VEGF receptor 2 ( VEGF R 2 ) and insulin receptor substrate ( IRS 1 ) and phosphatidylinositol 3 ' kinase ( PI3K ) was induced by VEGF , which augmented PI3K activity in VEGF R 2 immunoprecipitates . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin regulates glucose transport by activating insulin receptor substrate 1 ( IRS 1 ) dependent phosphatidylinositol 3 kinase ( PI3K ) which , via increases in PI 3 , 4 , 5 triphosphate ( PIP ( 3 ) ) , activates atypical protein kinase C ( aPKC ) and protein kinase B ( PKB / Akt ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
However , by also inhibiting an mTOR dependent serine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) , they may enhance insulin like growth factor 1 ( IGF 1 ) signaling and downstream phosphatidylinositol 3 kinase ( PI3K ) / AKT activation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Pull down assays with a fusion protein ( GST p85NC SH 2 ) , and coimmunoprecipitation studies , indicated that the insulin receptor substrate ( IRS ) , and not the epidermal growth factor and insulin like growth factor receptors or the Src tyrosine kinase , was physically associated with PI3K in these cells . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Since the negative consequence of chronic ET 1 exposure appears to be independent of signal disturbance along the insulin receptor substrate 1 / phosphatidylinositol ( PI ) 3 kinase ( PI3K ) / Akt 2 pathway of insulin action , we tested if ET 1 altered GLUT 4 regulation engaged by osmotic shock , a PI3K independent stimulus that mimics insulin action . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Glucose transport , insulin receptor ( IR ) , and IR substrate 1 ( IRS 1 ) phosphorylation , phosphatidylinositol 3 ' kinase ( PI3K ) activity , as well as Akt Ser 473 phosphorylation have been investigated at the end of the incubation period and after a further short term insulin stimulation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Genistein did not change the protein levels of insulin receptor substrate 1 ( IRS 1 ) , the p 85 regulatory subunit of phosphatidylinositol 3 kinase ( PI3K ) , or Akt . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We also demonstrate that caveolin 3 , a marker of caveolae , is required for the insulin receptor mediated activation of the PI3K dependent pathway , which occurs 2 min after insulin stimulation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin induced down regulation of apoM was blocked by AG 1024 ( a specific insulin receptor inhibitor ) and LY 294002 ( a phosphatidylinositol 3 kinase ( PI3K ) inhibitor ) , which indicates that it is mediated via the activation of PI3K pathway . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We also characterized insulin signaling pathways believed to promote myocyte growth and interact with proliferative responses mediated by G protein coupled receptors , and we assessed myocardial insulin receptor substrate 1 ( IRS 1 ) and p 110 alpha catalytic and p 85 regulatory subunits of phospatidylinositol 3 kinase ( PI3K ) , Akt , MEK , ERK1 / 2 , and S 6 kinase 1 ( S6K1 ) . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Denervation decreased the amounts of insulin receptor substrate 1 ( IRS 1 ) , phosphatidly inositol 3 phosphate kinase ( PI3K ) , and Akt 1 proteins in femora of Cbl b ( + / + ) mice , whereas the amounts of these IGF 1 signaling molecules in femora of Cbl b ( / ) mice were constant after denervation . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We examined signals that could activate muscle proteolysis and found low values of both phosphatidylinositol 3 kinase ( PI3K ) activity and phosphorylated Akt that were related to phosphorylation of serine 307 of insulin receptor substrate 1 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Molecular mechanisms of insulin resistance : serine phosphorylation of insulin receptor substrate 1 and increased expression of p85alpha : the two sides of a coin . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
Insulin stimulated Interaction between insulin receptor substrate 1 and p85alpha and activation of protein kinase B / Akt require Rab 5 . ^^^ This inhibition of PKB / Akt activation was because of the impaired interaction between insulin receptor substrate 1 and the p85alpha subunit of phosphatidylinositol 3 kinase . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
The detection of mRNAs for insulin receptor ( IR ) A and IRB ; insulin receptor substrate ( IRS ) 1 and IRS 2 ; phosphoinositide 3 kinase ( PI3K ) catalytic subunits p110alpha , p110beta , PI3KC2alpha , and PI3KC2gamma ; phosphoinositide dependent protein kinase 1 ; protein kinase B ( PKB ) alpha , PKBbeta , and PKBgamma in the beta cell population suggests the presence of a functional insulin signaling cascade in human beta cells . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
AGE 1 , a homolog of the p 110 catalytic subunit of phosphoinositide 3 kinases ( PI3K ) comprises the major known effector pathway downstream of the insulin receptor , DAF 2 . ^^^
Interacting proteins: P06213 and P27986 Pubmed SVM Score :0.0
We examined 35 genes predicted to have their major influence on insulin action , and three ( 9 % ) INSR , PIK3R1 , and SOS 1 showed significant associations with diabetes . ^^^