| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.78447221 |
| Cloning , chromosome localization , expression , and characterization of an Src homology 2 and pleckstrin homology domain containing insulin receptor binding protein hGrb10gamma . hGrb10alpha ( previously named Grb IR ) is a Src homology 2 domain containing protein that binds with high affinity to the tyrosine phosphorylated insulin receptor and insulin like growth factor 1 receptor . 0.78447221^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.90345923 |
| The association of pp60c src with insulin receptor in the synaptic membrane fraction , however , was temporally decreased after training . 0.90345923^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The predicted structure of c ros 1 is unique among the src family and clearly distinct from the human insulin receptor . . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 as a signaling molecule for focal adhesion kinase pp 125 ( FAK ) and pp 60 ( src ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Src homology 3 domain dependent interaction of Nck 2 with insulin receptor substrate 1 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The interaction with the activated insulin receptor is mediated by the Src homology 2 ( SH 2 ) domain of APS . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| H2O2 induced phosphorylation of ERK1 / 2 and PKB requires tyrosine kinase activity of insulin receptor and c Src . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In addition , a synthetic phosphopeptide ( 13 mer ) containing sequences conserved between the major tyrosine phosphorylation site of murine polyoma virus mT , hamster polyoma virus mT , and the insulin receptor substrate ( IRS 1 ) specifically blocked the association of the 85 and 110 kDa polypeptides with the mT pp60c src complex . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| A putative intracellular domain 435 amino acids long shows high homology with the SRC family of tyrosine kinases and within the kinase domain is most homologous with the human insulin receptor ( 44 % ) and 5 abl ( 41 % ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The 32P labeling of the insulin receptor beta subunit is increased by 2 3 fold when src kinase is included in the phosphorylation reaction . ^^^ The Mr = 93 , 000 receptor beta subunit and the Mr = 125 , 000 32P labeled bands are absent when an insulin receptor deficient sample , prepared by the inclusion of excess free insulin to inhibit the adsorption of the receptor to the insulin agarose , is phosphorylated in the presence of the src kinase . ^^^ These results indicate that the insulin receptor alpha and beta subunits are phosphorylated by the src kinase . ^^^ The src kinase catalyzed phosphorylation of the insulin receptor is not due to the activation of receptor autophosphorylation because a N ethylmaleimide treated receptor preparation devoid of receptor kinase activity is also phosphorylated by the src kinase . ^^^ Conversely , the insulin receptor kinase does not catalyze phosphorylation of the active or N ethylmaleimide inactivated src kinase . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation , catalyzed by either autophosphorylation or purified src kinase , of insulin receptor beta subunit in vitro activates the receptor kinase activity , whereas dephosphorylation with alkaline phosphatase deactivates the receptor kinase . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Under in vitro conditions , the tyrosine kinase activity of the insulin receptor toward histone is markedly activated when the receptor either undergoes autophosphorylation or is phosphorylated by a purified preparation of src tyrosine kinase on tyrosine residues of its beta subunit . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Immunological similarity between the insulin receptor and the protein encoded by the src oncogene . ^^^ Recently , we reported that there is immunological similarity between the insulin receptor and pp60src [ the protein encoded by the src oncogene of Rous sarcoma virus ( RSV ) ] . ^^^ Nevertheless , it seems unlikely that the insulin receptor gene is the proto oncogene for the src gene of RSV . . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Peptide 1150 served as a better substrate for the insulin receptor tyrosine protein kinase than either of the other peptides or than the Src peptide ( corresponding to the sequence surrounding the autophosphorylation site at Tyr 416 ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The peptide mapping of MAP 2 phosphorylated by the EGF receptor kinase and by the insulin receptor kinase produced very similar patterns of phosphopeptides , while that of MAP 2 phosphorylated by the src kinase gave a distinctly different pattern . ^^^ These results , together with our previous results , indicate that the substrate specificities of the EGF receptor kinase and the insulin receptor kinase are very similar , but not identical , while that of the src kinase is distinctly different from that of these growth factor receptor kinases . . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The complementary DNA sequence of the human insulin receptor is similar to those of the EGF receptor ( erbB oncogene ) and products of the src family of oncogenes . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| This gene shares structural and sequence homologies with the epidermal growth factor receptor ( erb B oncogene ) and members of the src family of oncogenes , suggesting that alterations in the insulin receptor , resulting from chromosomal translocation , could lead to a role in tumorigenesis . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin signal transduction involves the multisite docking protein insulin receptor substrate 1 ( IRS 1 ) and a number of Src homology 2 ( SH 2 ) domain factors , including p85 / p110 phosphatidylinositol 3 kinase , p 110 GTPase activating protein , and the phosphotyrosine specific phosphatase PTP1D . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Src homology 2 domains of protein tyrosine phosphatase are associated in vitro with both the insulin receptor and insulin receptor substrate 1 via different phosphotyrosine motifs . ^^^ To clarify the role of protein tyrosine phosphatase containing Src homology 2 ( SH 2 ) regions on insulin signaling , we investigated the interactions among the insulin receptor , a pair of SH 2 domains of SH PTP 2 coupled to glutathione S transferase ( GST ) and insulin receptor substrate 1 ( IRS 1 ) GST fusion protein ( amino portion , IRS IN ; carboxyl portion , IRS 1C ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic insulin receptor substrate 1 ( IRS 1 ) , which is multiply phosphorylated in vivo on tyrosine residues , is a known binding protein for the tandem src homology 2 ( SH 2 ) domain containing protein tyrosine phosphatase , SH PTP 2 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| A monoclonal antibody to a 60 kDa substrate of the insulin receptor tyrosine kinase is utilized in the present studies to examine this molecule in 3T3 cells expressing either the transforming chicken c Src ( mutant Phe 527 ) , the wild type molecule , or the parental cells . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Furthermore , insulin treatment of cells led to the association of the Src homology 2 domain of p95vav with the activated beta subunit of the insulin receptor in vitro . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Here , we describe p55PIK , a new regulatory subunit that was isolated by screening expression libraries with tyrosine phosphorylated insulin receptor substrate 1 ( IRS 1 ) . p55PIK is composed of a unique 30 residue NH 2 terminus followed by a proline rich motif and two Src homology 2 ( SH 2 ) domains with significant sequence identify to those in p 85 . p55PIK mRNA is expressed early during development , remains abundant in adult mouse brain and testis tissue , and is detectable in adult adipocytes and heart and kidney tissues . p55PIK forms a stable complex with p 110 , and it associates with IRS 1 during insulin stimulation . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The insulin receptor phosphorylates insulin receptor substrate 1 ( IRS 1 ) and Shc on tyrosine residues , both of which associate with the protein abundant Src homology / growth factor receptor bound protein 2 ( ASH / GRB2 ) leading to p21ras activation . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 ( IRS 1 ) and src homology and collagen protein ( SHC ) are signaling proteins which are rapidly phosphorylated on tyrosines after insulin receptor ( IR ) activation . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The association of the src homology 2 ( SH 2 ) domain containing tyrosine phosphatase ( SH PTP 2 ) with the activated epidermal growth factor ( EGF ) and platelet derived growth factor receptors , as well as the insulin receptor substrate 1 and growth factor receptor bound protein 2 and its intrinsic tyrosine phosphatase activity suggests an important role for this phosphatase in signal transduction . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Interferon alpha ( IFN alpha ) induces rapid tyrosine phosphorylation of the insulin receptor substrate 1 ( IRS 1 ) , a docking protein with multiple tyrosine phosphorylation sites that bind to the Src homology 2 ( SH 2 ) domains of various signaling proteins . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of the cytosolic Src homology 2 domain containing protein tyrosine phosphatase , SHPTP 2 , from insulin stimulated 3T3L1 adipocytes or Chinese hamster ovary cells expressing the human insulin receptor resulted in the coimmunoprecipitation of a diffuse tyrosine phosphorylated band in the 115 kDa protein region on SDS polyacrylamide gels . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| These peptides correspond to the 411 419 sequence of the Xenopus src oncogene , to the 1191 1220 sequence of the human EGF receptor precursor , to the 1146 1158 sequence of the human insulin receptor , to the 856 865 sequence of the human beta PDGF receptor , and to the 5 16 sequence of the erythrocyte human band 3 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Oncogenic activation of p 21 ( ras ) and pp 60 ( c src ) in human colonic Caco 2 cells decreases insulin receptor function and expression through protein kinase C dependent and independent pathways ] . ^^^ In conclusion , oncogenic p21ras and PyMT / pp60c src abolished insulin mitogenic signaling in Caco 2 cells through mechanisms involving ( 1 ) constitutive activation of MAP kinase , and ( 2 ) marked decreases in both insulin receptor function and expression which were mediated by PKC dependent and PKC independent pathways respectively . ^^^ This is the first evidence that , when oncogenically activated , p21ras and pp60c src not only exert a negative control on insulin receptor function but also repress insulin receptor gene expression in human colonic cells . . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Following insulin stimulation , SHPTP 2 , a non transmembrane protein tyrosine phosphatase , associates with insulin receptor substrate 1 via its Src homology 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| These proteins were found to be associated with the insulin receptor substrate 1 most likely through src homology ( SH 2 ) domains of these proteins . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| After phosphorylation by the insulin receptor , IRS 1 binds to the specific molecules which possess SH 2 ( src homology 2 ) domain such as 85 kDa subunit of phosphatidylinositol 3 kinase and may mediate insulin signals . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| SH PTP 2 is a nontransmembrane human protein tyrosine phosphatase that contains two Src homology 2 ( SH 2 ) domains and binds to insulin receptor substrate 1 ( IRS 1 ) via these domains in response to insulin . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Interactive roles of Ras , insulin receptor substrate 1 , and proteins with Src homology 2 domains in insulin signaling in Xenopus oocytes . ^^^ Insulin receptor substrate 1 ( IRS 1 ) serves as the major immediate substrate of insulin / insulin like growth factor ( IGF ) 1 receptors and following tyrosine phosphorylation binds to specific Src homology 2 ( SH 2 ) domain containing proteins including the p 85 subunit of phosphatidylinositol ( PI ) 3 kinase and GRB 2 , a molecule believed to link IRS 1 to the Ras pathway . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Involvement of Src homology / collagen ( SHC ) proteins in signaling through the insulin receptor and the insulin like growth factor 1 receptor . ^^^ Src homology / collagen ( SHC ) proteins are thought to participate in signaling through both receptor tyrosine kinases , such as the insulin receptor and the EGF ( epidermal growth factor ) receptor , and cytoplasmic tyrosine kinases , such as 5 src and 5 fps . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor kinase phosphorylates protein tyrosine phosphatase containing Src homology 2 regions and modulates its PTPase activity in vitro . ^^^ To clarify the role of protein tyrosine phosphatase ( PTPase ) containing a pair of Src homology 2 ( SH 2 ) regions upon insulin signaling , we studied the interactions between the insulin receptor and SH PTP 2 coupled to glutathione S transferase . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In particular , 5 Src was more tolerant of substitutions at the Met+1 and Met+3 positions in the YMXM motif than either 5 Abl or the insulin receptor kinase but was more dependent on the presence of a preceding acidic amino acid . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The rat and human insulin receptor substrate 1 ( IRS 1 ) is a specific target molecule for the insulin receptor beta subunit kinase and will bind to signal transducing molecules containing Src homology 2 domains through its multiple tyrosyl phosphorylation ( TP ) sites . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulates association of insulin receptor substrate 1 with the protein abundant Src homology / growth factor receptor bound protein 2 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Src homology 2 domains of protein tyrosine phosphatase are phosphorylated by insulin receptor kinase and bind to the COOH terminus of insulin receptors in vitro . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tyrosine residue 653 , which is in a homologous spatial position to major autophosphorylation sites in the catalytic domain of the src and insulin receptor kinases , is the major intracellular FGF R 1 phosphorylation site . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The catalytic activity of IGF 1 receptor is stimulated approximately 3 . 4 fold by treatment with purified 5 Src , while insulin receptor shows very little effect of Src phosphorylation under these conditions . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The insulin like growth factor 1 triggered signaling pathway involves autophosphorylation of the beta subunit of its tyrosine kinase receptor and phosphorylation of insulin receptor substrate 1 , the latter providing binding sites for proteins with src homology 2 domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Addition of exogenous P Tyr inhibited the insulin triggered insulin receptor ( IR ) tyrosine phosphorylation in the HEPG 2 cell line and the tyrosine phosphorylation of a variety of cellular proteins in src transformed NIH3T3 cells . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Increased tyrosine phosphorylation of these proteins is also observed in cells expressing a transforming chicken c Src ( mutant Phe 527 ) and in cells with the activated tyrosine kinase domains of the Drosophila insulin receptor , human insulin like growth factor 1 receptor , and human insulin receptor related receptor . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Concerning the insulin receptor cellular substrates , the anti ( 1294 1317 ) antibody inhibited Src homology / collagen and IRS 1 phosphorylation . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) by the activated receptors for insulin , IGF 1 , and various cytokines creates binding sites for signaling proteins with Src homology 2 domains ( SH 2 proteins ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Activated insulin and insulin like growth factor 1 receptors transmit downstream signals via the insulin receptor substrate ( IRS 1 and IRS 2 ) and a series of proteins containing Src homology 2 ( SH 2 ) domains , including SH 2 containing protein tyrosine phosphatase 2 ( SHP 2 ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 ( IRS 1 ) is rapidly phosphorylated on multiple tyrosine residues in response to insulin and binds several Src homology 2 domain containing proteins , thereby initiating downstream signaling . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Thus , SHPS 1 may be a direct substrate for both tyrosine kinases , such as the insulin receptor kinase or Src , and a specific docking protein for SH 2 domain containing PTPases . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The kinase specificity of roscovitine was investigated with 25 highly purified kinases ( including protein kinase A , G and C isoforms , myosin light chain kinase , casein kinase 2 , insulin receptor tyrosine kinase , c src , 5 abl ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Predominant expression of the src homology 2 containing tyrosine phosphatase protein SHP 2 in vascular smooth muscle cells . src homology 2 ( SH 2 ) containing protein tyrosine phosphatase SHP 2 is known to transduce positive signals from activated receptor protein tyrosine kinases such as platelet derived growth factor receptor ( PDGFR ) beta and insulin receptor . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| G971R is located near src homology 2 protein binding sites , and P170R and M209T are located in the phosphotyrosine binding domain of IRS 1 . 32D IR cells , stably overexpressing human insulin receptor , were transfected with wild type human IRS 1 cDNA ( WT ) or three mutant IRS 1 cDNAs and analyzed . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Although 5 src is a more potent inducer of tyrosine phosphorylation than c src 527 , the extent of phosphorylation of either insulin receptor substrate 1 or Shc , two of the major substrates of the IGF 1 receptor , does not seem sufficiently different to explain the qualitative difference in soft agar growth . 5 src , however , is considerably more efficient than c src 527 in its ability to tyrosyl phosphorylate , in R cells , the focal adhesion kinase , Stat 1 , and p130cas . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The active insulin receptor phosphorylates tyrosine residues of intracellular proteins such as the insulin receptor substrate 1 ( IRS 1 ) which acts as docking sites for molecules containing Src homology 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Following phosphorylation by the insulin receptor kinase , the insulin receptor substrates ( IRS ) 1 and IRS 2 bind to and activate several Src homology 2 ( SH 2 ) domain proteins . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Moreover , we studied the insulin mediated signal transduction pathways starting from insulin receptor , such as the Src kinase , the p21Ras / ERK , and the PI3K routes . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin binding to its receptor induces the phosphorylation of cytosolic substrates , insulin receptor substrate ( IRS ) 1 and IRS 2 , which associate with several Src homology 2 domain containing proteins . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Site directed mutagenesis and yeast two hybrid studies of the insulin and insulin like growth factor 1 receptors : the Src homology 2 domain containing protein hGrb 10 binds to the autophosphorylated tyrosine residues in the kinase domain of the insulin receptor . ^^^ Our findings suggest that , unlike other Src homology 2 domain containing proteins , hGrb 10 binds to the autophosphorylated tyrosine residues in the kinase domain of the insulin receptor , and the pleckstrin homology domain plays an important role in hGrb10 / receptor interaction . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The association of p 185 and p 60 with the src homology 2 ( SH 2 ) domains of Syp and the p 85 regulatory subunit of phosphatidylinositol 3 ' kinase was induced by bpV ( phen ) in HTC IR , but not in HTC M 1030 cells , as was insulin receptor substrate 1 associated phosphatidylinositol 3 ' kinase activity . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grb 10 protein was reported to bind to the insulin receptor and / or the insulin like growth factor ( IGF ) 1 receptor via its src homology 2 domain and to inhibit the associated tyrosine kinase activity that is involved in the growth promoting activities of insulin and IGFs ( IGF 1 and 2 ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor 1 receptor and insulin receptor association with a Src homology 2 domain containing putative adapter . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grb 10 interacts differentially with the insulin receptor , insulin like growth factor 1 receptor , and epidermal growth factor receptor via the Grb 10 Src homology 2 ( SH 2 ) domain and a second novel domain located between the pleckstrin homology and SH 2 domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 antisense expression in an hepatoma cell line reduces cell proliferation and induces overexpression of the Src homology 2 domain and collagen protein ( SHC ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The activated insulin receptor phosphorylates the intracellular substrate IRS 1 , which then binds various signalling molecules that contain SRC homology 2 domains , thereby propagating the insulin signal . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Inhibition of hGrb 10 binding to the insulin receptor by functional domain mediated oligomerization . hGrb 10 is a newly identified Src homology 2 ( SH 2 ) and pleckstrin homology ( PH ) domain containing protein that binds to autophosphorylated receptor tyrosine kinases , including the insulin and insulin like growth factor receptors . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In contrast , PD 166866 had no effect on c Src , platelet derived growth factor receptor beta , epidermal growth factor receptor or insulin receptor tyrosine kinases or on mitogen activated protein kinase , protein kinase C and CDK 4 at concentrations as high as 50 microM . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| It is activated in insulin stimulated cells by binding of the Src homology 2 ( SH 2 ) domains in its 85 kDa regulatory subunit to insulin receptor substrate 1 ( IRS 1 ) , and , others . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| CDK2 / cyclin A , insulin receptor tyrosine kinase and MAPK ) ; kinases in the active state ( e . g . casein kinase 1 , Lck ) ; and kinases in inactive states ( e . g . twitchin kinase , calcium calmodulin kinase 1 , FGF receptor kinase , c Src and Hck ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Two broad specificity biotinylated PTK peptide substrates were identified with optimum kinetic properties , allowing members from eight distinct classes of enzymes , including transmembrane ( epidermal growth factor receptor ( EGFR ) , fibroblast growth factor receptor , insulin receptor , and platelet derived growth factor receptor ) and cytoplasmic ( p 43 ( abl ) , p 56 ( lck ) , p 60 ( src ) , and p 93 ( fes ) ) PTKs , to be analyzed . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Other pathways activated by cytokine receptors : the mitogen activated protein kinase pathway ; insulin receptor substrates 1 and 2 and phosphatidylinositol 3 kinase pathways ; the Src pathways and other tyrosine kinase pathways ; the phospholipase C / protein kinase C / Ca2+ pathways . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In the two hybrid system , two domains of rGrb 14 can mediate the interaction with insulin receptors : the Src homology 2 ( SH 2 ) domain and a region between the PH and SH 2 domains that we named PIR ( for phosphorylated insulin receptor interacting region ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Effect of nutritional state on the formation of a complex involving insulin receptor IRS 1 , the 52 kDa Src homology / collagen protein ( Shc ) isoform and phosphatidylinositol 3 ' kinase activity . ^^^ The Src homology and collagen protein ( Shc ) is tyrosine phosphorylated in response to insulin ; however , evidence for its interaction with insulin receptor ( IR ) in normal tissues is missing . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We have extended these studies by demonstrating that paxillin , another focal adhesion protein , and Src undergo tyrosine dephosphorylation in response to insulin in Chinese hamster ovary ( CHO ) and rat hepatoma ( HTC ) cells that overexpress the insulin receptor . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In HIRY / F2 cells expressing a mutant insulin receptor lacking a binding site of SHP 2 , a protein tyrosine phosphatase containing src homology 2 ( SH 2 ) regions , insulin accelerated phosphorylation of Cas , as did IGF 1 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The polypeptide chain fold of CSK is most similar to c Src , Hck and fibroblast growth factor receptor 1 kinase ( FGFR1K ) and most dissimilar to insulin receptor kinase ( IRK ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We show that APS associates with phosphotyrosines situated within the activation loop of the insulin receptor via the APS Src homology 2 domain . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the lead dimethoxyquinazoline compound , WHI P 131 , which showed potent JAK 3 inhibitory activity ( IC 50 of 78 microM ) , did not inhibit JAK 1 and JAK 2 , the ZAP / SYK family tyrosine kinase SYK , the TEC family tyrosine kinase BTK , the SRC family tyrosine kinase LYN , or the receptor family tyrosine kinase insulin receptor kinase , even at concentrations as high as 350 microM . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We observed a rapid tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) and an increase in Src like kinase activity in anti IRS 1 immunoprecipitates from gastrin stimulated cells , suggesting that IRS 1 may be a direct substrate of Src . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In the present work , we have sought to assess the possible role of Src associated in mitosis 68 in insulin receptor signaling . ^^^ We performed in vivo studies in HTC IR cells and in vitro studies using recombinant Src associated in mitosis 68 , purified insulin receptor and fusion proteins containing either the N terminal or the C terminal Src homology 2 domain of p 85 phosphatidylinositol 3 kinase . ^^^ We have found that Src associated in mitosis 68 is a substrate of the insulin receptor both in vivo and in vitro . ^^^ Therefore , Src associated in mitosis 68 may be part of the signaling complexes of insulin receptor along with p 85 . ^^^ In vitro studies demonstrate that Src associated in mitosis 68 associates with the Src homology 2 domains of p 85 after tyrosine phosphorylation by the activated insulin receptor . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In vitro , Tub is phosphorylated by purified insulin receptor kinase as well as by Abl and JAK 2 but not by epidermal growth factor receptor and Src kinases . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The peptides contained either the YMTM motif found in insulin receptor substrate 1 ( IRS 1 ) or the YGEF motif identified from peptide library experiments to be the optimal sequence for Src . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| It is concluded that endogenous PTPalpha is a key regulator of c Src activity in 3T3 L 1 adipocytes and that PTPalpha is not required for the dephosphorylation of the insulin receptor or the insulin receptor substrate proteins or for the regulation of several downstream insulin signaling events in 3T3 L 1 adipocytes . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The hormone mediates these effects by activation of signaling pathways which utilize , 1 ) adaptor molecules such as the insulin receptor substrates ( IRS ) , the Src and collagen homologs ( Shc ) , and the growth factor receptor binding protein 2 ( Grb 2 ) ; 2 ) lipid kinases such as phosphatidylinositol 3 kinase ( PI 3 Kinase ) ; 3 ) small G proteins ; and 4 ) serine , threonine , and tyrosine kinases . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin signaling [ tyrosine phosphorylation of insulin receptor ( IR ) , insulin receptor substrate 1 ( IRS 1 ) , Src homology and collagen protein ( Shc ) and phosphatidyl inositol 3 ' kinase activity ( PI 3 ' kinase ) ] was studied in the liver and thigh muscles of fat ( FL ) and lean ( LL ) chickens . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Even at very high concentrations ranging from 175 350 microM , this inhibitor did not affect the enzymatic activity of other PTKs , including the Janus kinases JAK 1 and JAK 3 , the Src family kinase HCK , the Tec family member Bruton ' s tyrosine kinase , SYK kinase , and the receptor family PTK insulin receptor kinase . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The activated insulin receptor phosphorylates the intracellular substrate IRS protains , which then bind various signalling molecules that contain Src homology 2 domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| A series of hydroxynaphthalene pp 60 ( c src ) non peptide inhibitors was designed , using the crystal structure of the insulin receptor tyrosine kinase as a qualitative model , to target the peptide substrate binding site . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate 1 ( IRS 1 ) is a major substrate of the insulin receptor and acts as a docking protein for Src homology 2 domain containing signaling molecules that mediate many of the pleiotropic actions of insulin . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| As a consequence of IR inactivation , AT 2 receptor inhibits tyrosine phosphorylation of insulin receptor substrate 1 ( IRS 1 ) and signal regulatory protein ( SIRPalpha 1 ) and prevents subsequent association of both IRS 1 and SIRPalpha 1 with Src homology 2 ( SH 2 ) containing tyrosine phosphatase SHP 2 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We studied the impact of 16 serine residues in HIR by mutation to alanine and co overexpression in human embryonic kidney ( HEK ) 293 cells together with the docking proteins insulin receptor substrate ( IRS ) 1 , IRS 2 , or ( SHC ) Src homologous and collagen like . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with insulin and protein tyrosine phosphatase inhibitors such as vanadate and pervanadate resulted in the tyrosine phosphorylation of Grb 10 , a Src homology 2 ( SH 2 ) and pleckstrin homology domain containing adaptor protein which binds to a number of receptor tyrosine kinases including the insulin receptor ( IR ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin binding and protein levels of insulin receptor substrate 1 ( IRS 1 ) , p 85 subunit of phosphatidylinositol 3 ' kinase ( p 85 ) and Src homology 2 domain containing phosphatase 2 ( SHP 2 ) were then determined in hindlimb muscle and liver . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Since insulin is a key hormone for adipocyte conversion , and IRS 1 ( insulin receptor substrate 1 ) and Shc ( Src homology collagen ) proximal intracellular substrates of the insulin receptor control cell proliferation , the aim of this study was to investigate the role of IRS 1 and Shc in the early steps of differentiation . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Here we investigate the requirement of MAP ( MAPK ) and phosphatidylinositol 3 ( PI3K ) kinases in the transformation of rat intestinal epithelial ( RIE ) cells by oncogenic forms of insulin receptor ( gag IR ) , insulin like growth factor 1 receptor ( gag IGFR ) , and 5 Src . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| They act as an interface between multiple growth factor receptors possessing tyrosine kinase activity , such as the insulin receptor , and a complex network of intracellular signalling molecules containing Src homology 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In response to insulin , the following proteins were rapidly phosphorylated on tyrosine residues : 1 ) insulin receptor substrates 1 ( IRS 1 ) , 2 , and 4 ; 2 ) phospholipase C isoenzyme gamma ; 3 ) the Ras GTPase activating protein ( GAP ) associated with Rho GAP and p 62 ( Src ) ; 4 ) the insulin receptor beta subunit ; 5 ) the p 85 subunits of phosphatidylinositol 3 kinase ( PI 3 kinase ) ; 6 ) the Src homology 2 alpha collagen protein ; 7 ) protein kinase B ; 8 ) mitogen activated protein ( MAP ) kinase 1 and 2 ; and 9 ) growth receptor bound protein 2 . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| They act as docking proteins for the insulin receptor and a complex network of intracellular signalling molecules containing Src homology 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We assessed the roles of insulin receptor substrate 1 ( IRS 1 ) and Shc in insulin action on farnesyltransferase ( FTase ) and geranylgeranyltransferase 1 ( GGTase 1 ) using Chinese hamster ovary ( CHO ) cells that overexpress wild type human insulin receptors ( CHO hIR WT ) or mutant insulin receptors lacking the NPEY domain ( CHO DeltaNPEY ) or 3T3 L 1 fibroblasts transfected with adenoviruses that express the PTB or SAIN domain of IRS 1 and Shc , the pleckstrin homology ( PH ) domain of IRS 1 , or the Src homology 2 ( SH 2 ) domain of Shc . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion kinase and Src mediate integrin regulation of insulin receptor phosphorylation . ^^^ Conversely , expression of dominant negative mutants of either Src or FAK abolishes potentiation of insulin receptor phosphorylation by cell adhesion . ^^^ The results suggest that both Src and FAK participate in integrin mediated regulation of insulin receptor signal . . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Down regulation of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc gene expression by insulin in skeletal muscle is not associated with insulin resistance or type 2 diabetes . ^^^ To examine whether altered gene expression of insulin receptor substrates ( IRS ) 1 and IRS 2 and Src homologous and collagen like protein Shc is an inherited trait and is associated with muscle insulin resistance or type 2 diabetes , we measured mRNA levels of these genes by a relative quantitative RT PCR method in muscle biopsies taken before and after an insulin clamp from 12 monozygotic twin pairs discordant for type 2 diabetes and 12 control subjects . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Treatment with 17 AAG decreased NPM ALK expression and phosphorylation , thus impairing its association with phospholipase C gamma , Src homology 2 domain containing protein ( Shc ) , growth factor receptor bound protein 2 ( Grb 2 ) , and insulin receptor substrate 1 ( IRS 1 ) . ^^^ |
|
| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Here we show that PTP1B mutants that are defective in Src homology 3 domain binding fully retain the ability to inhibit insulin signalling , whereas mutants defective in insulin receptor binding fully retain the ability to inhibit integrin signalling . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| On the other hand , within the protein tyrosine kinases involved in the phosphorylation of CaM are receptors with tyrosine kinase activity , such as the insulin receptor and the epidermal growth factor receptor , and nonreceptor protein tyrosine kinases , such as several members of the Src family kinases , Janus kinase 2 , and p38Syk . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Signalling by the insulin receptor substrate ( IRS ) proteins is critically dependent on the tyrosine phosphorylation of specific binding sites that recruit Src homology 2 ( SH 2 ) domain containing proteins , such as the p 85 subunit of phosphoinositide 3 kinase ( PI 3 kinase ) , the tyrosine phosphatase SHP 2 and the adapter protein Grb 2 . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Studies of receptor type specificity showed that SMA 41 : ( a ) preferentially inhibited the kinase activity of EGFR over those of Src , insulin receptor and protein kinase C ( PKC , a serine / threonine kinase ) , ( b ) induced stronger inhibition of growth stimulated with EGF than of growth stimulated with platelet derived growth factor ( PDGF ) or fetal bovine serum ( FBS ) . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| We show that phosphoinositide 3 ( PI 3 ) kinase associates with 4 tyrosine phosphorylated proteins in primary human erythroid progenitors , namely insulin receptor substrate 2 ( IRS 2 ) , Src homology 2 domain containing inositol 5 ' phosphatase ( SHIP ) , Grb 2 associated binder 1 ( Gab 1 ) , and the Epo receptor ( EpoR ) . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Adapter protein with a pleckstrin homology ( PH ) and an Src homology 2 ( SH 2 ) domain ( APS ) and SH 2 B enhance insulin receptor autophosphorylation , extracellular signal regulated kinase and phosphoinositide 3 kinase dependent signalling . ^^^ Adapter protein with a pleckstrin homology ( PH ) and an Src homology 2 ( SH 2 ) domain ( APS ) and SH 2 B are adapter proteins and substrates that interact with the activation loop of the insulin receptor ( IR ) kinase . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Upon JAK 2 activation , tyrosine phosphorylation of signal transducer and activator of transcription 1 ( STAT 1 ) , STAT 5b , insulin receptor substrate 1 ( IRS 1 ) , and Src homology and collagen homology ( Shc ) were detected . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Novel espin actin bundling proteins are localized to Purkinje cell dendritic spines and bind the Src homology 3 adapter protein insulin receptor substrate p 53 . ^^^ The insulin receptor substrate p 53 ( IRSp 53 ) , an Src homology 3 ( SH 3 ) adapter protein and regulator of the actin cytoskeleton , was identified as an espin binding protein in yeast two hybrid screens . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor phosphorylated IRS5 / DOK4 associates with RasGAP , Crk , Src , and Fyn , but not phosphatidylinositol 3 kinase p 85 , Grb 2 , SHP 2 , Nck , or phospholipase Cgamma Src homology 2 domains , and activates MAPK in cells . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| However , VEGFR 2 , insulin receptor substrate 1 , and c Src were also involved in VEGF A induced activation of PI 3 kinase , resulting in the compensation in cells expressing kinase inactive c Fes . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the phosphorylated insulin receptor interacting region ( PIR ) and Src 2 homology domains ( SH 2 ) of Grb 7 and Grb 14 were differently implicated in the inhibition of FGFR signalling . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grb 10 is a Pleckstrin homology and Src homology 2 ( SH 2 ) domain containing protein that binds to the tyrosine phosphorylated insulin receptor in response to insulin stimulation . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Akt survival pathway in insulin producing RINm5F cells by arousing Src to activate insulin receptor substrate 1 . ^^^ We also show that Src directed activation of insulin receptor substrate 1 , phosphatidylinositol 3 kinase ( PI3K ) , Akt , and Bad phosphorylation conform a substantial component of the survival route because pharmacological inhibition of PI3K and Akt canceled the antiapoptotic effects of NO . ^^^ Studies performed with the protein kinase G ( PKG ) inhibitor KT 5823 revealed that NO dependent activation of c Src / insulin receptor substrate 1 is not affected by PKG activation . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| APS ( adapter protein with Pleckstrin homology and Src homology 2 domains ) is recruited by the autophosphorylated insulin receptor and is essential for Glut 4 translocation . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| However , association of the insulin receptor and IRS 1 with the Src homology 2 domain of the p 85 subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) was reduced by ethanol . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The protein and mRNA expression of IGF 1 receptor ( IGF IR ) and insulin receptor substrate ( IRS ) 1 , but not Src homology / collagen protein , increased in response to 1 microM genistein in a time dependent manner . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| The insulin receptor is a transmembrane tyrosine kinase that is essential for mediating multiple intracellular signalling cascades that lead ultimately to the biological actions of insulin Tyrosine phosphorylation o f the cytosolic proteins insulin receptor substrate 1 and 2 ( IRS 1 and IRS 2 ) produces protein ' scaffolding ' for the assembly of effector proteins containing Src homology 2 ( SH 2 ) domains , thereby generating multisubunit signalling complexes . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Gab 2 ( Grb 2 associated binder 2 ) , a member of the IRS ( insulin receptor substrate ) / Gab family of adapter proteins , undergoes tyrosine phosphorylation in response to cytokine or growth factor stimulation and serves as a docking platform for many signal transduction effectors , including the tyrosine phosphatase SHP 2 [ SH 2 ( Src homology 2 ) domain containing tyrosine phosphatase ] . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| In addition , the novel espin isoforms of sensory cells differed from other espin isoforms in that they potently inhibited actin polymerization in vitro , did not bind the Src homology 3 domain of the adapter protein insulin receptor substrate p 53 , and did not bind the acidic , signaling phospholipid phosphatidylinositol 4 , 5 bisphosphate . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| With the use of an in vitro pull down technique , the src homology 3 ( SH 3 ) domain of insulin receptor substrate p 53 ( IRSp 53 ) precipitated WAVE 2 from platelet lysates more efficiently than did profilin 1 . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| SH 2 B , an Src homology 2 ( SH 2 ) and pleckstrin homology domain containing adaptor protein , binds via its SH 2 domain to insulin receptor in response to insulin ; however , its physiological role remains unclear . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grb 14 has been shown to bind to these activated RTKs through its Src homology 2 ( SH 2 ) domain , with the exception of the insulin receptor , where the primary binding interaction is via a small domain adjacent to the SH 2 domain ( the BPS or PIR domain ) . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Between the PH ( pleckstrin homology ) and SH 2 ( Src homology 2 ) domains is a new binding domain implicated in the interaction with receptor tyrosine kinases called PIR ( phosphorylated insulin receptor interaction region ) . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| TCDD causes suppression of growth and differentiation of MCF10A , human mammary epithelial cells by interfering with their insulin receptor signaling through c Src kinase and ERK activation . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Pull down assays with a fusion protein ( GST p85NC SH 2 ) , and coimmunoprecipitation studies , indicated that the insulin receptor substrate ( IRS ) , and not the epidermal growth factor and insulin like growth factor receptors or the Src tyrosine kinase , was physically associated with PI3K in these cells . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Deletion mutagenesis on Grb 14 indicated a phosphorylated insulin receptor interacting ( PIR ) domain between the PH ( pleckstrin homology ) and SH 2 ( Src homology ) domains that binds to IRbeta . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Comparison of the exon structure of the tyrosine kinase domain of the INSR with the corresponding regions of the human SRC , ROS , and ERBB 2 ( NGL ) protooncogenes indicates that the exon intron organization of this region has not been well conserved . . ^^^ |
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| Interacting proteins: P06213 and P12931 |
Pubmed |
SVM Score :0.0 |
| Compound 4e inhibited the FGFr tyrosine kinase with an IC 50 of 0 . 060 microM , whereas IC50s for the inhibition of the PDGFr , FGFr , EGFr , c src , and InsR tyrosine kinases for this compound ( 4e ) were all greater than 50 microM . . ^^^ |
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