| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :1.0394584 |
| Since this effect occurs only when LAR is eutopically expressed at the cell membrane , these data further suggest that LAR requires a transmembrane localization to directly interact with the insulin receptor in situ . . 1.0394584^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.96381866 |
| A physical association between LAR and the insulin receptor was then shown by immunoprecipitation of LAR from cell lysates and immunoblotting with antibody to the insulin receptor , or vice versa . 0.96381866^^^ Functional association between the insulin receptor and the transmembrane protein tyrosine phosphatase LAR in intact cells . 0.73143651^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.54766497 |
| These results are further evidence that LAR is a physiological regulator of the insulin receptor and is consistent with its direct interaction with the tyrosine phosphorylated insulin receptor . . 0.54766497^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :1.1632291 |
| The Cys 1522 to Ser mutant protein , which is defective in the LAR PTPase domain 1 catalytic site , was tightly associated with tyrosine phosphorylated insulin receptor , but failed to dephosphorylate it , indicating that LAR PTPase domain 1 is critical for dephosphorylation of tyrosine phosphorylated insulin receptor . 1.1632291^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| The PTPN 1 gene codes for protein tyrosine phosphatase 1B ( PTP1B ) ( EC 3 . 1 . 3 . 48 ) , which negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of the insulin receptor kinase activation segment . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Of the three PTPase examined , only immunodepletion of LAR protein from the homogenates with neutralizing antibodies resulted in normalization of the PTPase activity towards the insulin receptor , demonstrating that the increase in LAR was responsible for the enhanced PTPase activity in the adipose tissue from obese subjects . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| To test the hypothesis that the transmembrane PTPase LAR can modulate insulin receptor signaling in vivo , antisense RNA expression was used to specifically suppress LAR protein levels by 63 % in the rat hepatoma cell line , McA RH 7777 . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| In order to explore the potential role of the transmembrane PTPase ( LAR ) in insulin receptor signal transduction , we overexpressed the full length LAR protein in McA RH 7777 rat hepatoma cells and found that modest increases in the abundance of LAR protein expression downregulated a number of insulin stimulated cellular responses closely related to the activation of the receptor kinase . ^^^ An increase in LAR protein of 2 . 4 fold over the level in control cells caused a 40 % reduction in insulin receptor autophosphorylation in intact cells , without an alteration in insulin receptor mass or a change in the insulin stimulated receptor kinase activity measured with partially purified receptors in vitro . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Because of the potential role of the transmembrane PTPase LAR in the regulation of insulin signaling , we assessed the effect of 3S peptide 1 on recombinant LAR PTPase activity and in McA RH 7777 rat hepatoma cells overexpressing full length LAR protein ( McA4B / LAR ) . 3S peptide 1 significantly reduced insulin receptor dephosphorylation by recombinant LAR ( p < 0 . 001 ) while blocking dephosphorylation of the insulin receptor by approximately 72 % in semi permeabilized McA4B / LAR cells ( p < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| The mass of the insulin receptor beta subunit and the PTPases PTP1B and leukocyte antigen related was not significantly different between the two adipose depots . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NMR analysis of regioselectivity in dephosphorylation of a triphosphotyrosyl dodecapeptide autophosphorylation site of the insulin receptor by a catalytic fragment of LAR phosphotyrosine phosphatase . ^^^ To begin to examine recognition of triphosphotyrosyl sites by protein tyrosine phosphatases in possible control of signal transduction a triphosphotyrosyl dodecapeptide TRDIpYETDpYpYRK corresponding to residues 1 , 142 1 , 153 of the insulin receptor was prepared and incubated with the 40 kDa catalytic domain of the human PTPase LAR . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| We evaluated the deactivation of the insulin receptor kinase by three candidate enzymes that are expressed in insulin sensitive rat tissues , including the receptor like PTPases LAR and LRP , and the intracellular enzyme , PTPase1B . ^^^ The accelerated deactivation of the insulin receptor kinase by LAR and its relative preference for regulatory phosphotyrosine residues further support a potential role for this transmembrane PTPase in the physiological regulation of insulin receptors in intact cells . . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Here , insulin dependent insulin receptor tyrosine kinase activation was examined with recombinant insulin receptor substrate 1 ( IRS 1 ) as the substrate and shown to be 3 fold greater in cells with suppressed LAR levels . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Consistent with these data , insulin receptor signaling is increased when the CD 45 related PTPase LAR is reduced by antisense suppression in a rat hepatoma cell line . ^^^ To test whether the hematopoietic cell specific PTPase CD 45 functions in a manner similar to LAR by negatively modulating insulin receptor signaling in hematopoietic cells , the insulin responsive human multiple myeloma cell line U 266 was isolated into two subpopulations that differed in CD 45 expression . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| In order to determine whether the effects of TNF alpha might involve alterations in the expression of specific protein tyrosine phosphatases ( PTPases ) that have been implicated in the regulation of growth factor receptor signalling , KRC 7 rat hepatoma cells were treated with TNF alpha , and changes in overall tissue PTPase activity and the abundance of three major hepatic PTPases ( LAR , PTP1B , and SH PTP 2 ) were measured in addition to effects of TNF alpha on ligand stimulated autophosphorylation of insulin and epidermal growth factor ( EGF ) receptors and insulin stimulated insulin receptor substrate 1 ( IRS 1 ) phosphorylation . ^^^ Furthermore , decreased abundance of the LAR PTPase , which has been implicated in the regulation of insulin receptor phosphorylation , may account for the less marked effect of TNF alpha on the autophosphorylation state of the insulin receptor while postreceptor actions of insulin are inhibited . . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Previous studies indicate that LAR antisense transfectants demonstrate increased insulin receptor signaling . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , PTP LAR , previously suggested to be a major regulator of the insulin receptor tyrosine kinase , was expressed in extremely low levels in skeletal muscle , whereas the related receptor type PTP sigma and PTP alpha were expressed in relatively high levels in all four tissues . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| We found that the transmembrane enzymes , protein tyrosine phosphatase ( PTP ) alpha and leukocyte common antigen related ( LAR ) , were detected predominantly in the plasma membrane and to a lesser extent in the heavy microsomes , a distribution similar to that of insulin receptor . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| PTP1B is a cytosolic protein tyrosine phosphatase that is a regulator of the kinase activity of the insulin receptor ; the two protein tyrosine phosphatases LAR and CD 45 are receptor type phosphatases crucially important to cell function . ^^^ LAR also is involved in regulation of the insulin receptor while CD 45 is critical for T cell activation . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| Finally , considerable data support a role for LAR in negatively regulating the insulin receptor signaling . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| To test the role of the leukocyte common antigen related protein tyrosine phosphatase ( LAR ) as a regulator of insulin receptor ( IR ) signalling , an siRNA probe against LAR was developed . ^^^ The phosphorylation and dephosphorylation of the IR and insulin receptor substrate ( IRS ) proteins were unaffected by LAR knock down . ^^^ |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P06213 |
Pubmed |
SVM Score :0.0 |
| NA |
|