Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P05121 and P04004 Pubmed SVM Score :1.3008655
Both in plasma and in the growth substratum of cultured endothelial cells , PAI 1 is associated with its binding protein vitronectin , resulting in a stabilization of active PAI 1 . 1.3008655^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.51309456
The PAI 1 binding domain ( s ) were not destroyed by this treatment , since the digested vitronectin competed with immobilized vitronectin for PAI 1 binding to the same extent as uncleaved vitronectin . 0.51309456^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.66699981
In view of the apparent association between PAI 1 and vitronectin in the endothelial cell matrix ( ECM ) , we analyzed the interaction between PAI 1 and thrombin in this environment . 0.66699981^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.53116742
We demonstrate that although urea treated vitronectin binds PAI 1 with an affinity greater than that of native vitronectin , it does not accelerate the rate of inactivation of alpha thrombin by PAI 1 . 0.53116742^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.63387364
In these areas , PAI 1 appeared to colocalized with its binding protein vitronectin . 0.63387364^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.50521473
Furthermore , experiments in which PAI 1 failed to compete with heparin for binding to vitronectin argue for separate binding sites for the two ligands on vitronectin . . 0.50521473^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :1.065181
We propose that vitronectin binds active PAI 1 by interaction via amino acid residues that originate from distal locations in the N and C termini of vitronectin . . 1.065181^^^ Derivatives of vitronectin obtained by specific cleavage at its cluster of basic amino acids with thrombin , elastase and plasmin are shown to have a decreased ability to bind plasminogen activator inhibitor 1 ( PAI 1 ) . 0.86918027^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.77619719
Vitronectin ( VN ) binds to plasminogen activator inhibitor 1 ( PAI 1 ) and integrins and may play an important role in the vascular response to injury by regulating fibrinolysis and cell migration . 0.77619719^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.50417388
The predominant associating species observed was high in molecular weight ( M ( r ) approximately 320 , 000 ) , demonstrating that self association of vitronectin occurs upon interaction with PAI 1 . 0.50417388^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.65669801
We propose that PAI 1 acts as a positive switch for angiogenesis by promoting endothelial cell migration away from their vitronectin containing perivascular space toward fibronectin rich tumor tissue . 0.65669801^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.50307408
The latter often involve interactions between PAI 1 and vitronectin or the urokinase receptor . 0.50307408^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.87814664
In blood , PAI 1 is bound to the adhesion protein vitronectin and is associated with vitronectin in fibrin clots and the provisional matrix . 0.87814664^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.83105316
The interaction of plasminogen activator inhibitor 1 ( PAI 1 ) with its binding protein vitronectin ( VN ) ( Declerck , P . 0.83105316^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.6630933
The adhesion protein vitronectin is associated with extracellular matrices and serves as cofactor for plasminogen activator inhibitor 1 . 0.6630933^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.62580048
Vitronectin , an adhesive blood glycoprotein , has the potential to modulate this system by its known capacity to interact with plasminogen activator inhibitor 1 , plasminogen activators , the urokinase plasminogen activator receptor , and plasminogen . 0.62580048^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The experiments were performed in the presence and absence of vitronectin , a multifunctional protein which has been shown to bind PAI 1 in plasma and in the matrix of endothelial cells . ^^^ The second order rate constants for t PA inhibition of `` wild type ' ' PAI 1 and PAI P 1 P1 ' ATIII , irrespective of the presence of vitronectin , were similar , whereas replacing P 3 P3 ' resulted in a 40 fold decrease of the second order rate constant towards t PA , again independent of vitronectin . ^^^ In the absence of vitronectin , reactivity of PAI 1 and its `` antithrombin 3 like ' ' variants towards thrombin was slow ; however , PAI 1 P 3 P3 ' ATIII had a 10 fold higher k 1 than wild type PAI 1 ( 1 . 3 10 10 ( 4 ) M 1 s 1 versus 1 . 1 10 10 ( 3 ) M 1 s 1 ) . ^^^ In contrast , in the presence of vitronectin , PAI 1 and even more rapidly PAI 1 P 3 P3 ' ATIII were found to be effective thrombin inhibitors , with k 1 values of 2 . 2 10 10 ( 5 ) M 1s 1 and 1 . 8 10 10 ( 6 ) M 1 s 1 , respectively . ^^^ Thus , in the presence of vitronectin , PAI 1 P 3 P3 ' ATIII displays a 3 fold higher k 1 with thrombin than with t PA . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Anti vitronectin antibodies , which block PAI 1 binding to the matrix , abrogate the inhibitory effect of PAI 1 on monocyte invasiveness , indicating that active PAI 1 is bound via matrix associated vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In this review , we discuss the properties of PAI 1 and the evidence that the binding of PAI 1 to ECM is mediated by serum derived vitronectin ( Vn ) . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In normal plasma , PAI 1 occurs in three molecular forms , a Mr greater than 700 KDa inactive form of heterogeneous composition , an active 450 KDa form containing PAI 1 / vitronectin complex and an inactive peak at Mr 50 KDa containing free PAI 1 . ^^^ It is concluded that in subjects with increased PAI 1 levels in plasma , PAI 1 may occur as high molecular weight complexes with vitronectin of which 450 KDa was the most important part and as a 50 KDa inactive form ; t PA circulates primarily in complex with inhibitors . ^^^ Thus , some of the molecular interactions between PAI 1 , t PA and vitronectin , previously demonstrated in purified systems in vitro , also occur in plasma . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We have now investigated whether receptor bound uPA also retains the ability to react with and be inhibited by plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . uPA bound to its receptor on human U 937 monocyte like cells was inhibited by PAI 1 ( in its active form in the presence of vitronectin fragments ) with an association rate constant of 4 . 5 10 10 ( 6 ) M 1 s 1 , which was 40 % lower than that obtained for uPA in solution ( 7 . 9 10 10 ( 6 ) M 1 s 1 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The isolated PAI 1 BP was shown to be identical to S protein ( vitronectin ) both by cross reactivity with monospecific rabbit antisera and by NH 2 terminal amino acid sequence analysis . ^^^ The gel filtration behavior , mobility on sodium dodecyl sulfate gel electrophoresis , and concentration in plasma suggest that PAI 1 BP is a multimer ( presumably a dimer ) of S protein accounting for approximately 35 % of the S protein in plasma . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI ) is bound to vitronectin in plasma . ^^^ NH 2 terminal amino acid sequence analysis and immunoblotting analysis suggested that the two compounds were PAI ( 50 kDa ) and vitronectin ( 75 kDa ) . ^^^ We conclude that the PAI binding protein is identical to vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Demonstration of the binding , stabilization , and activation of PAI 1 by vitronectin . ^^^ In contrast , the PAI activity in HT 1080 medium concentrate declines rapidly on standing . 2 ) Hep G 2 PAI 1 invariably copurified with an adhesive protein , vitronectin or its NH 2 terminal fragment , while pure HT 1080 PAI 1 alone was obtained by affinity purification on anhydrourokinase Sepharose 4B . 3 ) Based on specific activity measurement and complex formation analysis using a sodium dodecyl sulfate ( SDS ) polyacrylamide gel electrophoresis technique , the purified Hep G 2 PAI 1 appears completely active while the HT 1080 PAI 1 is only one fourth as active . ^^^ Purified vitronectin was found to enhance and stabilize the PAI 1 activity of the partially active HT 1080 PAI 1 . ^^^ It is concluded that fully active PAI 1 in association with vitronectin can be isolated by anhydrourokinase Sepharose 4B chromatography and that vitronectin is a binding protein for PAI 1 which activates and stabilizes PAI 1 . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We conclude that the high Mr form of PAI constitutes a complex between 50 , 000 Mr PAI and vitronectin from fetal calf serum . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Neither the extent nor the time course of complex formation was altered by using PAI 1 that had been pre incubated with native human vitronectin , compared with native PAI 1 alone . 125I scu PA . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Significantly higher levels of t PA and PAI pre venous occlusion and significantly lower levels of antithrombin 3 , C protein and S protein were observed in Group 1 as compared to controls . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Antithrombin 3 , C protein ( CP ) , plasminogen , plasminogen activator inhibitor ( PAI ) , total S protein ( TSP ) , free S protein ( FSP ) , lupus anticoagulant ( LA ) and anticardiolipin antibodies ( ACA ) type IgG were determined in all patients . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Recombinant PAI 1 also promoted SK N BE ( 2 ) cell detachment from vitronectin and migration from vitronectin toward fibronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Differences in PAI 1 expression did not affect the attachment of these cell lines to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Although antibodies to PAI 1 did not affect the adhesion of HT 1080 cells to vitronectin , the antibody to uPA reduced their attachment . ^^^ Addition of exogenous PAI 1 , however , prevented HT 1080 cell adhesion ( IC 50 180 nM ) and promoted cell detachment from vitronectin . ^^^ Furthermore melanoma cells transfected with a uPA variant , which had an impaired interaction with PAI 1 , were not invasive and had impaired binding to vitronectin . ^^^ These data also suggest that uPA and PAI 1 may co operate in the migratory process by respectively facilitating the attachment to , and subsequent detachment from , vitronectin in the extracellular matrix . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
SMCs replated on vitronectin after culture on monomer collagen result in PAI 1 accumulation at focal adhesions and colocalization with alpha ( 5 ) beta 3 integrins . ^^^ In contrast , polymerized collagen inhibits PAI 1 accumulation at focal adhesions when the SMCs are replated on vitronectin . ^^^ Furthermore , for SMCs cultured on polymerized collagen , platelet derived growth factor stimulated migration on vitronectin is enhanced by PAI 1 , with its function counteracted by urinary plasminogen activator . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
To test the hypothesis that during wounding , exposed collagen , the most abundant ECM molecule in the skin , regulates keratinocyte PA and PAI gene expression , we utilized an in vitro model in which activated keratinocytes were cultured in dishes coated with collagen or other ECM substrates . tPA , uPA , and PAI 1 mRNA and enzymatic activity were detected when activated keratinocytes attached to fibronectin , vitronectin , collagen 4 , and RGD peptide . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 inhibition enhanced vitronectin dependent transendothelial migration of human bone marrow derived CD34+ cells , and resulted in a striking augmentation of angioblast dependent neovascularization . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Moreover , downregulation of PAI 1 significantly enhanced the ability of endothelial cells to adhere to vitronectin , and this effect could be reversed upon addition of recombinant PAI 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( complement S protein , serum spreading factor , epibolin ) is a multifunctional glycoprotein that mediates cell to substrate adhesion , inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family , viz . antithrombin 3 , plasminogen activator inhibitor 1 ( PAI 1 ) and 2 ( PAI 2 ) , heparin cofactor 2 and protease nexin . ^^^ Vitronectin ( complement S protein , serum spreading factor , epibolin ) is a multifunctional glycoprotein that mediates cell to substrate adhesion , inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family , viz . antithrombin 3 , plasminogen activator inhibitor 1 ( PAI 1 ) and 2 ( PAI 2 ) , heparin cofactor 2 and protease nexin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Inhibition of invasion by PAI 1 is abolished by presaturating the amnion membranes with antiitronectin monoclonal antibody ( 10 micrograms / ml ) which prevents binding of PAI 1 to tissue associated vitronectin . ^^^ Binding of PAI 1 to tissue vitronectin is therefore a prerequisite for its inhibitory action . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) has been implicated as a major matrix associated regulator component of plasminogen activation by serving as a potent stabilizing cofactor of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Complexes of urokinase and plasminogen activator inhibitor type 1 ( PAI 1 ) were detected in cell layers of vitronectin adherent but not fibronectin adherent cells . ^^^ Inhibition of PAI 1 using a neutralizing monoclonal antibody resulted in a 3 fold increase in urokinase enzymatic activity on vitronectin adherent cells . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Functionally active PAI 1 is bound to a discrete binding or carrier protein in plasma , which was recently identified as vitronectin . ^^^ In the present study , the interaction between PAI 1 and vitronectin has been studied in purified systems and in plasma by agarose gel electrophoresis using non denaturing conditions and by crossed immunoelectrophoresis using an antiserum produced towards purified PAI 1 / vitronectin complex . ^^^ Virtually all of the purified vitronectin , which did not contain any appreciable amounts of polymerized material , and almost all of the vitronectin in plasma , had the capacity to form a complex with PAI 1 . ^^^ In contrast to functionally active PAI 1 , latent or chloramine T inactivated PAI 1 did not form such a complex with vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Matrix samples were fractionated by SDS PAGE and analyzed by PAI 1 ligand binding and by immunoblotting using antibodies to vitronectin ( Vn ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Immunoblotting of substrate attached material indicated that HT 1080 cells deposited PAI 1 into vitronectin , but not fibronectin , dependent contacts . ^^^ These data suggest that the antibody mediated cell rounding resulted from a steric disruption of vitronectin dependent adhesions , indicating that the binding site on vitronectin for PAI 1 is near , but does not overlap , the binding site for vitronectin receptor . ^^^ The accumulation of PAI 1 into vitronectin dependent adhesion sites correlated temporally with the preferential degradation of fibronectin from the substrate . ^^^ In the presence of a neutralizing antibody against PAI 1 , vitronectin adherent cells activated plasminogen to the same extent as fibronectin adherent cells . ^^^ These data represent the first demonstration that vitronectin associated PAI 1 regulates urokinase in focal contact areas . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Experiments suggest that the strained loop of PAI 1 is not responsible for the transition between the latent and the active conformations or for binding to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The interaction between type 1 plasminogen activator inhibitor ( PAI 1 ) and fragments of vitronectin ( Vn ) was investigated . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We now report that vitronectin ( VN ) mediates the specific binding of PAI 1 to the ECM of these cells . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Fibrinogen , heparin , and vitronectin enhance the rate of inhibition of t PA by PAI 1 . ^^^ Kinetic studies described here demonstrate that Lp ( a ) prevents the inhibition of t PA by PAI 1 in the presence of fibrinogen and heparin , but has no effect on the reaction in the presence of vitronectin or in the absence of either fibrinogen or heparin . ^^^ The data suggest that fibrinogen and heparin may enhance the rate of inhibition through an interaction with t PA , and that vitronectin may enhance the inhibition through an interaction with PAI 1 . ^^^ These data suggest that PAI 1 inhibition of t PA in vivo is primarily mediated via interaction with fibrinogen , heparin , vitronectin , and Lp ( a ) , and therefore , the functional levels of PAI 1 activity in the vasculature may be regulated by the presence of these components . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In vivo , PAI 1 is encountered in complex with vitronectin , an interaction known to stabilize its activity but not to affect the second order association rate constant ( k 1 ) between PAI 1 and t PA . ^^^ Nevertheless , by using PAI 1 reactive site variants ( R346M , M347S , and R346M M347S ) , we show that the binding of vitronectin to the PAI 1 mutant proteins improves plasminogen activator inhibition . ^^^ In the absence of vitronectin the PAI 1 R346M mutants are virtually inactive toward t PA ( k 1 less than 1 10 10 ( 3 ) M 1 s 1 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the current work we have analyzed the regulation of vitronectin by transforming growth factor beta 1 ( TGF beta 1 ) in this hepatoma cell line by Northern hybridization , polypeptide and immunoprecipitation analyses and compared the response to another TGF beta regulated gene , plasminogen activator inhibitor ( PAI 1 ) . ^^^ Comparison of the temporal TGF beta induction profiles of vitronectin and PAI 1 mRNAs showed that vitronectin was induced more slowly but the vitronectin mRNAs persisted longer . ^^^ The results indicate that TGF beta 1 is an efficient regulator of the production of vitronectin by HepG 2 cells and that PAI 1 and vitronectin are not coordinately regulated . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
As a result of the plasmin cleavage , the affinity between vitronectin and the type 1 inhibitor of plasminogen activator ( PAI 1 ) is significantly reduced . ^^^ A mechanism is proposed through which plasmin can arrest its own production by feedback signalling , unleashing PAI 1 from the immobilized vitronectin found in the vascular subendothelium , which becomes exposed at the locus of a hemostatic event . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the presence of either the protein vitronectin or the glycosaminoglycan heparin , PAI 1 is also an efficient inhibitor of thrombin . ^^^ To assess whether these cofactors turn PAI 1 into a general protease inhibitor or whether their influence is restricted to thrombin , the second order association rate constants between PAI 1 and the human plasma proteases t PA , u PA , plasmin , thrombin , Factor Xa ( FXa ) , and Factor XIIa ( FXIIa ) in the absence and in the presence of either vitronectin or heparin are determined . ^^^ Our results show that : ( 1 ) Heparin exclusively increases the rate of inhibition of thrombin by PAI 1 , whereas in the presence of heparin the rate of inhibition of the other proteases is not altered ; ( 2 ) Vitronectin is an obligatory cofactor for the inhibition of thrombin by PAI 1 . ^^^ In addition , vitronectin moderately increases the rate of inhibition by PAI 1 of u PA and of plasmin , but does not alter the rate of inhibition of t PA , FXa , or FXIIa ; ( 3 ) Apart from the important role of the P 1 residue , no consensus can be presented on the nature of other residues within the P 3 to P 3 ' region with regard to target protease specificity . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) stabilizes plasminogen activator inhibitor type 1 ( PAI 1 ) activity and prevents the fibrin ( ogen ) induced acceleration of plasminogen activation by t PA . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Type 1 inhibitor of plasminogen activators ( PAI 1 ) occurs in purified preparations in a latent form that can be activated with denaturants ; in vivo , latency is prevented by binding to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 binds to a cell adhesion molecule , vitronectin . ^^^ The association of PAI 1 with vitronectin appears to stabilize PAI 1 . ^^^ PAI 1 in complex with vitronectin is still accessible to plasminogen activators . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Addition of the PAI 1 binding protein , vitronectin , to reactivated PAI 1 , about doubled the half life of PAI 1 at all conditions studied . ^^^ The stability of the spontaneously active high Mr form of PAI 1 ( partially purified or in plasma ) , constituting a complex between PAI 1 and vitronectin , was quite similar to reactivated PAI 1 in the presence of vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
E . coli derived PAI 1 , added to human plasma and fractionated by Sephacryl S 200 chromatography , eluted in two peaks that were similar to those obtained with guanidine activated PAI 1 from eucaryotic cells , suggesting that it bound to the PAI 1 binding protein ( vitronectin ) . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Since PAI 2 has structural homology to PAI 1 , but does not bind to vitronectin , chimeras of PAI 1 and PAI 2 were constructed . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We recently localized the high affinity binding site for activated type 1 plasminogen activator inhibitor ( PAI 1 ) to the somatomedin B domain ( i . e . amino acid ( aa ) 1 51 ) of vitronectin ( Vn ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In plasma and the extracellular matrix , PAI 1 is associated with the adhesive glycoprotein vitronectin . ^^^ In order to characterize the PAI 1 structural domain responsible for binding to vitronectin , the segment of the PAI 1 cDNA encoding amino acids 13 147 ( nucleotides 248 650 ) was randomly mutagenized and subcloned into a bacterial expression vector containing the mature PAI 1 coding sequence . ^^^ Recombinant PAI 1 mutants were expressed in Escherichia coli and bacterial lysates assayed in duplicate for uPA inhibitory activity and vitronectin binding . ^^^ These results suggest that vitronectin binding may stabilize the active conformation of PAI 1 by restricting the movement of beta sheet A and thereby preventing insertion of the reactive center loop . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The production of the PAI 1 binding protein vitronectin ( Vn ) was not affected by HGF . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Identification of a PAI 1 binding site in vitronectin . ^^^ Active PAI 1 ( plasminogen activator inhibitor 1 ) is bound to vitronectin in plasma and in the extracellular matrix . ^^^ In this study we aimed at identifying the PAI 1 binding site in vitronectin , which at present is a matter of dispute . ^^^ Vitronectin was cleaved with trypsin and the fragments were tested for inhibitory effect on the PAI 1 / vitronectin interaction using vitronectin coated microtiter plates . ^^^ Intact vitronectin and the tryptic digest of vitronectin both caused a 50 % reduction in PAI 1 binding at a concentration of about 2 nmol / I . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Determination of the vitronectin binding site on plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Vitronectin is the carrier protein of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ We used a well characterized panel of anti human PAI 1 monoclonal antibodies ( MoAbs ) to localize the vitronectin binding site on PAI 1 . ^^^ By employing a direct vitronectin / PAI 1 binding assay and two vitronectin dependent inhibition assays , we demonstrate that the anti PAI 1 MoAbs CLB 5 , CLB 10 , CLB 2C8 and I 1 , directed against different epitopes in the region between amino acids 110 and 145 , prevent the interaction of PAI 1 with vitronectin . ^^^ We conclude that the region between amino acids 110 and 145 of PAI 1 harbours an important determinant for the interaction with vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Such a transconformation may be used for targeting the PKA phosphorylation to vitronectin molecules bound to GAGs , for example in the extracellular matrix or on cell surfaces ; ( f ) From the biochemical point of view ( Km values and physiological concentrations ) the phosphorylation of vitronectin can take place at the locus of a hemostatic event ; ( g ) The phosphorylation of Ser 378 in vitronectin alters its function , since it significantly reduces its ability to bind the inhibitor 1 of plasminogen activator ( s ) ( PAI 1 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
A series of 8 peptides derived from the amino acid sequence accommodating the plasmin cleavage site in vitronectin were synthesized and used to map its binding site for the type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^ These results account for our previous finding that cleavage of the R 361 S362 bond by plasmin significantly reduces the affinity between PAI 1 and vitronectin , since it splits the PAI 1 binding site in two . ^^^ Furthermore , in the case of the two chain form of vitronectin , this cleavage detaches the S 362 R379 peptide which provides some of the affinity elements for the binding of PAI 1 . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We have shown that a heptapeptide which resides in the middle part of vitronectin ( VN ) is responsible for binding to plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The loss of inhibitor is not secondary to diminished vitronectin ( Vn ) , the primary binding protein of PAI 1 in the ECM , but appears to be secondary to decreased synthesis at the RNA level . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In early atherosclerotic lesions PAI 1 was also detected in intimal SMCs and in extracellular areas in association with vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Neither vitronectin , known to stabilize PAI 1 , nor vitronectin mRNA increased in liver . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
However , the capacity of the peptide to inhibit PAI 1 bound to vitronectin , a plasma protein that stabilizes PAI 1 activity , was markedly attenuated . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In addition , we describe the relationship between vitronectin ( VN ) and plasminogen activator inhibitor 1 ( PAI 1 ) in the self regulating mechanism of TGF beta action for fibrogenesis in acute viral hepatitis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
This PAI 1 binding protein was isolated from human plasma and identified as vitronectin ( S protein ) . ^^^ Subsequently , it was shown that vitronectin is also the major protein responsible for binding of active PAI 1 to the extracellular matrix , thereby suggesting that vitronectin may also play a role in the regulation of cell associated fibrinolytic or proteolytic processes ( e . g . cell migration , tumor metastasis . . . ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
This review describes the biochemistry of PAI 1 including its purification , conversion between active and latent forms , and interaction with its target serine proteases and its protein cofactor , vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 activity in the conditioned media was zero under all conditions , which was indicative of the rapid inactivation and degradation of PAI 1 known to occur in media devoid of vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the presence of either the glycoprotein vitronectin or the glycosaminoglycan heparin , PAI 1 is endowed with additional , efficient thrombin inhibitory properties ( Ehrlich et al . , 1990 , 1991a ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Although combined ligand blot / immunoblot analysis revealed that vitronectin was the major PAI 1 binding protein in platelets , vitronectin / PAI 1 complexes were not detected in alpha granules using a two site immunoassay . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 alone , a poor solution phase inhibitor of thrombin , can efficiently inhibit thrombin in the presence of native vitronectin ( VN ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) and its cofactor vitronectin ( Vn ) are stored within the alpha granules of platelets . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) binds to the somatomedin B ( SMB ) domain of vitronectin ( VN ) , a domain present in at least seven other proteins . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Cleaved PAI 1 did not bind to either PAs or vitronectin but retained the heparin binding capacity . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
No association between the distribution of PAI 1 and vitronectin was found , and no specific signal for tissue type PA , urokinase type PA , tumor necrosis factor alpha , or interleukin 1 was detected . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The isolated peptides bound directly to plasminogen activator inhibitor 1 ( PAI 1 ) and were effective competitors of the interaction of PAI 1 with isolated intact vitronectin or extracellular matrix . ^^^ These natural peptides may interact with active PAI 1 in plasma or at extravascular sites and thereby interfere with established biological functions of intact vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) ( assayed by ELISA , n = 9 ) , the PAI 1 binding protein , vitronectin ( immunoprecipitation , n = 3 ) and tissue factor ( ELISA , n = 3 ) were not affected by LIF , but fibrinogen production increased up to twofold with LIF ( 500 U / ml ; Western blot , n= 3 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In this study , the localization of plasminogen activator inhibitor 1 ( PAI 1 ) and its association with glomerular subepithelial deposits of vitronectin ( VN ) in membranous nephropathy ( MN ) was evaluated . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , a member of serine proteinase inhibitor superfamily , is known to inhibit thrombin in the presence of either heparin or vitronectin . ^^^ Apparent second order rate constant ( ki ) for the inhibition of factor Xa by PAI 1 at 5 mM Ca2+ was 1 . 6 10 10 ( 4 ) M 1 s 1 , and was enhanced 3 fold by 2 u / ml of heparin ( 4 . 6 10 10 ( 4 ) M 1 s 1 ) and 10 fold by 100 nM vitronectin ( 1 . 6 10 10 ( 5 ) M 1 s 1 ) , respectively . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the primary physiologic inhibitor of plasminogen activation , is associated with the adhesive glycoprotein vitronectin ( Vn ) in plasma and the extracellular matrix . ^^^ In this study we examined the binding of different conformational forms of PAI 1 to both native and urea purified vitronectin using a solid phase binding assay . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) is an obligatory cofactor for the inhibition of thrombin by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In gel filtration rpoPAI 1 in buffer eluted at a volume corresponding to 24 kDa , whereas in the presence of porcine plasma , the molecular form containing PAI 1 activity eluted at a volume corresponding to 330 kDa , presumably as a consequence of binding of active PAI 1 to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Preincubation of plasma vitronectin with plasminogen activator inhibitor 1 ( PAI 1 ) , which provoked multimer formation , induced the binding of vitronectin to osteonectin . ^^^ In a concentration dependent fashion , PAI 1 , CaCl 2 , heparin and heparan sulphate , but not other glycosaminoglycans , interfered with the binding of vitronectin to osteonectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 specifically interfered with intergrin and vitronectin mediated migration : Migration onto vitronectin coated but not onto fibronectin coated surfaces was inhibited by PAI 1 , a cyclic RGD peptide inhibited migration , and both cell lines expressed vitronectin binding alpha 5 integrins . ^^^ In addition , active PAI 1 , but not latent or reactive center cleaved PAI 1 , inhibited vitronectin binding to integrins in an in vitro binding assay , without affecting binding of fibronectin . ^^^ We conclude that PAI 1 , independently of its role as a proteinase inhibitor , inhibits cell migration by competing for vitronectin binding to integrins , while the interference of PAI 1 with binding of vitronectin to the urokinase receptor may play a secondary role . ^^^ These data define a novel function for the serpin PAI 1 , enabling it to regulate cell migration over vitronectin rich extracellular matrix in the body . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Serum deprived human myogenic cells were plated in serum free medium onto bacteriologic dishes precoated with different extracellular matrix ligands ( fibronectin , vitronectin , or type 1 collagen ) or PAI 1 at increasing concentrations . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Recently , mechanisms of molecular crosstalk between these receptor systems have been recognized : ( a ) uPA receptor may directly interact with beta 1 and beta 2 integrins on circulating blood cells ; ( b ) av beta 3 integrin directly binds to a matrix metalloproteinase ; ( c ) uPA and PAI 1 balance the high affinity binding of vitronectin to uPA receptor ; ( d ) vitronectin dependent cell adhesion and migration involving alpha 5 integrins or uPA receptor are blocked by active PAI 1 independent of its role as protease inhibitor . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Because vitronectin also binds plasminogen activator inhibitor type 1 ( PAI 1 ) , and plasmin cleavage of vitronectin reduces PAI 1 binding , we explored the effects of plasmin and PAI 1 on the interaction between uPAR and vitronectin . ^^^ PAI 1 blocked cellular binding of and adhesion to vitronectin by over 80 % ( IC 50 approximately 5 nM ) , promoted detachment of uPAR bearing cells from vitronectin , and increased cellular migration on vitronectin . ^^^ These data illuminate a complex regulatory scheme for uPAR dependent cellular adhesion to vitronectin : Active urokinase promotes adhesion and also subsequent detachment through activation of plasmin or complex formation with PAI 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The PAI 1 data obtained were compared with the vitronectin and the beta thromboglobulin contents in platelets from the same individuals . ^^^ However , using a newly developed ELISA method for PAI 1 vitronectin complex we failed to demonstrate such a complex in the platelet lysates . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Therefore , thrombin will efficiently inactivate PAI 1 in the presence of either vitronectin or heparin , unless a sufficient excess of the inhibitor is present . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
METHODS : We examined the patterns of expression of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin in head and neck squamous cell carcinomas using immunohistochemical techniques . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
BACKGROUND : We examined the relationships among vitronectin ( VN ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and transforming growth factor beta 1 ( TGF beta 1 ) in liver diseases to evaluate the presence of plasmin cascade in human hepatic fibrosis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The purified recombinant mutant proteins , rPAI 1 / R78A K124A and rPAI 1 / K82A R120A , produced by the yeast Pichia pastoris , were indistinghuisable from wild type recombinant and natural human PAI 1 with respect to inhibitory activity against uPA , stability of SDS resistant complexes with uPA , and vitronectin binding . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The PAI 1 variants have been characterized by their reactivity with tissue plasminogen activator ( tPA ) , interactions with vitronectin or heparin , and stability . ^^^ Nevertheless a similar affinity between all the active PAI 1 variants and vitronectin was observed . ^^^ Our results do not support the presence of a binding site for vitronectin in this part of the molecule , but rather that it might be involved in controlling the active PAI 1 to substrate transition . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the present report we localize the expression of placental PAI 1 in greater detail and compare it with that of tissue factor ( TF ) , a procoagulant molecule , and vitronectin ( Vn ) , a PAI 1 cofactor . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The close proximity of binding sites for alpha ( 5 ) integrins and plasminogen activator inhibitor 1 ( PAI 1 ) on vitronectin may have consequences for cell adhesion and migration , or for the localized inhibition of plasminogen activators . ^^^ In this study , the kinetics and reversibility of vitronectin dependent cell adhesion via alpha ( 5 ) integrins was investigated using RGD peptides and PAI 1 as competitors . ^^^ Active , but not latent or cleaved PAI 1 , and RGD peptides were effective in preventing cell adhesion to vitronectin provided the inhibitor was present at the time of cell seeding . ^^^ Following cell seeding onto a vitronectin substratum , delayed addition of RGD peptides or active PAI 1 ( 10 20 min post seeding ) resulted in the loss of their inhibitory potential . ^^^ These data were supported by experiments in a purified system where delayed addition of active PAI 1 could no longer prevent vitronectin binding to immobilized alpha ( 5 ) beta 3 , while a cyclic RGD peptide gave some moderate inhibition . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Its effect on human PAI 1 was not significantly inhibited by 1 microg ml ( 1 ) fibrin or a approximately 7 fold molar excess of vitronectin ( 1 nM ) . ^^^ Inhibition of rat PAI 1 was unchanged by fibrin , but vitronectin reduced inhibition from 0 . 5 nM . 4 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
It circulates in plasma complexed with vitronectin ( VN ) , the primary PAI 1 binding protein . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In a concentration dependent manner , active , but not latent or complexed , PAI 1 abrogated cell adhesion onto vitronectin but not onto fibrinogen or other matrix substrata . ^^^ The direct binding of vitronectin , but not of other matrix proteins , to integrin alphaIIb beta 3 was blocked by active PAI 1 in a purified system . ^^^ Since activated platelets release active and latent PAI 1 as well as structurally and functionally distinct forms of vitronectin , the described interactions appear to be physiologically significant . ^^^ Co distribution of vitronectin and PAI 1 at sites of fibrin polymers within platelet thrombi was demonstrated by transmission electron microscopy , suggesting an extracellular functional relationship of both release products with regard to cell adhesion . ^^^ Furthermore , megakaryocyte maturation may depend on the intact vitronectin integrin adhesion system that is influenced by PAI 1 , thereby proposing a regulatory role for the inhibitor in cellular differentiation . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 also has a role in regulating cell adhesion processes relevant to tissue remodeling and metastasis ; this role is mediated by its binding to the adhesive glycoprotein vitronectin rather than by proteinase inhibition . ^^^ CONCLUSIONS : The structure clarifies the molecular basis of the stabilizing mutations and the reduced affinity of PAI 1 , on cleavage or in the latent form , for vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
ECV 304 cells display further characteristics of endothelial cells , including the expresssion of thrombomodulin and of the vitronectin receptor CD 51 , as well as the secretion of plasminogen activator inhibitor 1 ( PAI 1 ) and endothelin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The cofactors heparin , vitronectin ( VN ) , and thrombomodulin ( TM ) modulate the reactivity of alpha thrombin with plasminogen activator inhibitor ( PAI 1 ) . ^^^ Analysis of these results suggest that ( 1 ) heparin binds to exosite 2 of alpha thrombin to accelerate the reaction by a template mechanism , ( 2 ) VN accelerates PAI 1 inactivation of alpha thrombin by lowering the K ( d ) for initial complex formation by an unknown mechanism that does not require binding to either exosite 1 or exosite 2 of alpha thrombin , ( 3 ) alpha thrombin may have a binding site for PAI 1 within or near exosite 1 , and ( 4 ) TM occupancy of exosite 1 partially accounts for the protection of thrombin from rapid inactivation by PAI 1 in the presence of vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In contrast to active wtPAI 1 and other substrate type PAI 1 mutants , PAI 1 delhF showed a 3000 fold decreased binding to vitronectin . ^^^ Moreover , removal of helix F strongly affects the binding of PAI 1 to vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) , an abundant plasma and matrix glycoprotein , binds PAI 1 and stabilizes its active conformation . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Attachment to vitronectin was also inhibited in a dose dependent manner by the type 1 plasminogen activator inhibitor ( PAI 1 ) , a serine protease inhibitor . ^^^ Our data demonstrate that unstimulated primary neonatal rat astrocytes attach to vitronectin , utilizing integrins alphavbeta 5 and alpha8beta1 , and that this attachment is regulated by PAI 1 . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Colocalization of thrombin , PAI 1 , and vitronectin in the atherosclerotic vessel wall : A potential regulatory mechanism of thrombin activity by PAI 1 / vitronectin complexes . ^^^ By using immunofluorescence confocal microscopy , we demonstrate that the antigens of thrombin , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin ( Vn ) colocalize in human neointimal atherosclerotic arterial tissue . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) , the primary inhibitor of tissue type plasminogen activator ( t PA ) , circulates as a complex with the abundant plasma glycoprotein , vitronectin . ^^^ This interaction stabilizes the inhibitor in its active conformation In this report , the effects of vitronectin on the interactions of PAI 1 with fibrin clots were studied . ^^^ Confocal microscopic imaging of platelet poor plasma clots reveals that essentially all fibrin associated PAI 1 colocalizes with fibrin bound vitronectin . ^^^ Moreover , formation of platelet poor plasma clots in the presence of polyclonal antibodies specific for vitronectin attenuated the inhibitory effects of PAI 1 on t PA mediated fibrinolysis . ^^^ Addition of vitronectin during clot formation markedly potentiates PAI 1 mediated inhibition of lysis of ( 125 ) 1 labeled fibrin clots by t PA . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Mab 2 , but not Mab 6 , prevented vitronectin from rescuing PAI 1 from cold induced substrate behaviour . ^^^ The potentiating effect of vitronectin on neutralization of PAI 1 by antibodies is a novel concept in the development of compounds for neutralizing PAI 1 in vivo . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
As PAI 1 regulates motility through the interaction of vitronectin with its receptor , the integrin alphaVbeta 3 , we identified this receptor in human preadipocytes . ^^^ Functional studies indicated that active , but not latent , PAI 1 inhibited preadipocyte attachment to vitronectin with an IC ( 50 ) of 13 . 3 nmol / L , and preincubation of vitronectin coated Transwells with active PAI 1 prevented preadipocyte migration . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Stable PAI 1 bound to vitronectin was cleaved and inactivated by MMP 3 in a manner comparable with that of free PAI 1 ; however , the cleaved protein did not bind to vitronectin . ^^^ Cleavage and inactivation of PAI 1 by MMP 3 may thus constitute a mechanism decreasing the antiproteolytic activity of PAI 1 and impairing the potential inhibitory effect of vitronectin bound PAI 1 on cell adhesion and / or migration . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 is also a component of the ECM , where it binds to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
A complex of plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 1 binding protein ( PAI 1 BP ) contained S protein ( vitronectin ) , PAI 1 and unidentified 40 kDa protein on SDS PAGE under reducing conditions . ^^^ Therefore , it was thought that PAI 1 BP consisted of S protein and SP 40 , 40 . ^^^ The results clearly indicated that SP 40 , 40 stabilized PAI 1 activity as well as S protein did . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Such complexes could be also detected by a solid phase binding assay ; and ( c ) the real time bimolecular interactions monitored by surface plasmon resonance indicated that the complex of alpha ( 1 ) acid glycoprotein with PAI 1 is less stable than that formed by vitronectin with PAI 1 , but in both cases , the apparent K ( D ) values were in the range of strong interactions ( 4 . 51 + 1 . 33 and 0 . 58 + 0 . 07 nm , respectively ) . ^^^ The on rate for binding of PAI 1 to alpha ( 1 ) glycoprotein or vitronectin differed by 2 fold , indicating much faster complex formation by vitronectin than by alpha ( 1 ) acid glycoprotein . ^^^ On the other hand , dissociation of PAI 1 bound to vitronectin was much slower than that from the alpha ( 1 ) acid glycoprotein , as indicated by 4 fold lower k ( off ) values . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) is bound to vitronectin ( VN ) in plasma and in the extracellular matrix . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) binds to the somatomedin B ( SMB ) domain of vitronectin . ^^^ Although PAI 1 blocked the adhesion of all of these cells to vitronectin coated wells , it did not block adhesion to a variant of vitronectin which lacked the SMB domain . ^^^ These results affirm that PAI 1 can inhibit both uPAR and integrin mediated cell adhesion , and demonstrate that the SMB domain of vitronectin is required for these effects . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We report here that amino acid substitutions in a buried cluster of three residues forming a hydrogen bonding network in the shutter region drastically accelerate PAI 1 latency transition ; that the rate was in all cases normalized by the PAI 1 binding protein vitronectin ; and that substitution of an adjacent beta strand 5A Lys residue , believed to anchor beta strand 5A to other secondary structural elements , had differential effects on the rates of latency transition in the absence and the presence of vitronectin , respectively . ^^^ These findings show that vitronectin regulates the movements of the RCL through conformational changes of the shutter region and beta strand 5A , are in agreement with RCL insertion proceeding by different routes during latency transition and complex formation , and contribute to the biochemical basis for the potential use of PAI 1 as a therapeutic target in cancer and cardiovascular diseases . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 circulates in complex with vitronectin ( Vn ) , an interaction that stabilizes PAI 1 in its active conform . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Interaction of plasminogen activator inhibitor type 1 ( PAI 1 ) with vitronectin . ^^^ In addition to inhibiting serine proteases , mainly tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators , PAI 1 interacts with different components of the extracellular matrix , i . e . fibrin , heparin ( Hep ) and vitronectin ( Vn ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The plasminogen activator inhibitor 1 ( PAI 1 ) is stabilized in its inhibitory conformation by binding to Vitronectin ( Vn ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( Vn ) stabilizes the inhibitory form of plasminogen activator inhibitor 1 ( PAI 1 ) , an important modulator of fibrinolysis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
One way that PAI 1 influences these processes may be mediated through its binding to the plasma protein vitronectin . ^^^ Binding to PAI 1 results in the incorporation of vitronectin into a higher order complex with a potential for multivalent interactions ( Podor , T . ^^^ In this study , evidence is provided to support this concept from studies on the effects of PAI 1 induced multimerization on the interactions of vitronectin with matrix components and cell surface receptors . ^^^ By monitoring complex formation and stability over time using size exclusion high performance liquid chromatography , a correlation is made between PAI 1 induced multimerization and enhanced cell / matrix binding properties of vitronectin . ^^^ This evidence indicates that PAI 1 alters the adhesive functions of vitronectin by converting the protein via the higher order complex to a self associated , multivalent species that is functionally distinct from the abundant monomeric form found in the circulation . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Moreover , there is accumulating evidence that both the urokinase receptor and PAI 1 are multifunctional proteins involved not only in extracellular matrix proteolysis but also in cellular adhesion and migration through their binding site for vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Using specific PAI 1 mutants , we could demonstrate that PAI 1 promotes angiogenesis at physiological ( nanomolar ) concentrations through its antiproteolytic activity rather than by interacting with vitronectin . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 also regulates the attachment of cells to the adhesive glycoprotein vitronectin ( VN ) . ^^^ Our experiment indicates that vitronectin and fibronectin , as components of ECM , may be the factors involved in the regulation of PAI 1 gene expression . ^^^ PAI 1 , as an inhibitor of the interaction between vitronectin and integrin alphavbeta 3 , may also be a regulator of its own expression . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Stable PAI 1 bound to vitronectin is cleaved and inactivated by MMP 3 in a comparable manner as free PAI 1 ; the cleaved protein , however , does not bind to vitronectin . ^^^ Cleavage and inactivation of PAI 1 by MMP 3 may thus constitute a mechanism decreasing the antiproteolytic activity of PAI 1 and impairing the potential inhibitory effect of vitronectin bound PAI 1 on cell adhesion and / or migration . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Moreover , a few non proteolytic functions have been ascribed to PAI 1 , mediated by its interaction with vitronectin or the interaction between the uPA PAI 1 complex bound to the uPA receptor and members of the low density lipoprotein receptor family . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The NH ( 2 ) terminal somatomedin B ( SMB ) domain ( residues 1 44 ) of human vitronectin contains eight Cys residues organized into four disulfide bonds and is required for the binding of type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^ Plasmon surface resonance ( BIAcore ) and competitive enzyme linked immunosorbent assays demonstrate that the purified rSMB domain and intact urea activated vitronectin have similar PAI 1 binding activities . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the serpin plasminogen activator inhibitor 1 ( PAI 1 ) , RCL movements are regulated by vitronectin , having a previously poorly defined binding site lateral to PAI 1 ' s beta sheet A . ^^^ Using a novel strategy , based on identification of amino acid residues necessary for vitronectin protection of PAI 1 against inactivation by 4 , 4 ' dianilino 1 , 1 ' bisnaphthyl 5 , 5 ' disulfonic acid , we have defined a vitronectin binding surface spanning 10 residues between alpha helix F , beta strand 2A , and alpha helix E . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We examined the roles of vitronectin and plasminogen activator inhibitor 1 ( PAI 1 ) in neointima development . ^^^ Because intravascular fibrin may function as a provisional matrix for invading VSMCs , we examined potential mechanisms by which vitronectin and PAI 1 regulate fibrin stability and fibrin VSMC interactions . ^^^ Inhibition of activated protein C by PAI 1 was markedly attenuated in vitronectin deficient plasma . ^^^ The capacity of PAI 1 to inhibit clot lysis was significantly attenuated in vitronectin deficient plasma , and this effect was not explained simply by the PAI 1 stabilizing properties of vitronectin . ^^^ The adhesion and spreading of VSMCs were significantly greater on wild type plasma clots and PAI 1 deficient plasma clots than on vitronectin deficient plasma clots . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The adhesive glycoprotein vitronectin ( VN ) forms a function stabilizing complex with plasminogen activator inhibitor 1 ( PAI 1 ) , the major fibrinolysis inhibitor in both plasma and vessel wall connective tissue . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Specifically , exogenously added PAI 1 inhibited cell adhesion to vitronectin but not fibronectin , in agreement with the literature . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Interaction of plasminogen activator inhibitor type 1 ( PAI 1 ) with vitronectin ( Vn ) : mapping the binding sites on PAI 1 and Vn . ^^^ In plasma as well as in the extracellular matrix PAI 1 binds to vitronectin ( Vn ) , an interaction that affects the function of both proteins . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Overexpression of plasminogen activator inhibitor 1 ( PAI 1 ) reduces tumor cell migration in vitro and metastasis in mice in vivo by mechanisms involving either inhibition of urokinase plasminogen activator ( u PA ) activity or competition for an integrin binding site on vitronectin . ^^^ The mutant PAI 1 proteins were defective in either vitronectin binding ( PAI 1 ( VN ) ) , plasminogen activator inhibition ( PAI 1 ( INH ) ) or both ( PAI 1 ( VN , INH ) ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
OBJECTIVE : We previously reported that plasminogen activator inhibitor 1 ( PAI 1 ) , in the presence of vitronectin ( VN ) , inhibits thrombin activity in vitro . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Do PAI 1 and vitronectin promote or inhibit neointima formation . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Moreover , there is an accumulating evidence that both the urokinase receptor and PAI 1 are multifunctional proteins involved not only in extracellular matrix proteolysis , but also in cellular adhesion and migration through their binding site for vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , competes with alphavbeta 3 integrin for vitronectin ( VN ) binding . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The inhibition of angiogenesis could be related to 1 ) an increase in genes involved in the inhibition of cell proliferation ( p 19 ( INK 4 ) , p 21 ( Waf / Cip1 ) , Wnt 5a ) , the inhibition of cell migration ( Rho GDI 1 , alpha E catenin ) and 2 ) a downregulation of genes involved in angiogenesis ( PAI 1 , Vitronectin , HoxD 3 , Notch 4 ) or in cell invasion ( Semaphorin E ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Absence of the uPAR scavenger receptor was associated with significantly greater accumulation of plasminogen activator inhibitor 1 protein ( PAI 1 ) ( 20 . 5 + / 3 . 5 versus 9 . 1 + / 2 . 9 % area , day 14 UUO ) and vitronectin protein ( 2 . 4 + / 1 . 1 versus 0 . 9 + / 0 . 4 % area , day 14 UUO ) . ^^^ By immunostaining alphaSMA+ cells , CD34+ cells , vitronectin and PAI 1 co localized to the same tubulointerstitial area . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) is shown to partially relieve TM inhibitory action only by vastly increasing the initial rate of interaction between free thrombin and PAI 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) and two chain high molecular weight kininogen ( HKa ) exert anti adhesive properties in vitronectin dependent cell adhesion . ^^^ PAI 1 or HKa induced a 2 to 3 fold increase in apoptosis of human umbilical vein endothelial cells ( HUVEC ) and vascular smooth muscle cells ( VSMC ) adherent to vitronectin , as determined by annexin 5 FACS assay , similar to alphav integrin inhibitor cyclo ( Arg Gly Asp D Phe Val ) peptide ( cRGDfV ) . ^^^ In contrast , PAI 1 and HKa did not affect fibronectin dependent adhesion or cell survival . uPA did not influence apoptosis in vitronectin or fibronectin adherent cells . ^^^ In atherosclerotic vessel sections , congruent distribution of vitronectin , PAI 1 , HK , and of components of the urokinase plasminogen activator / receptor system with apoptotic cells lining foam cell lesions was demonstrated by immunostaining . ^^^ These results indicate that inhibition of vitronectin dependent cell adhesion through PAI 1 and HKa correlates with apoptosis induction in vascular cells mediated through the caspase 3 pathway . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The double mechanism of action of PAI 1 ( proteolytic activity inhibition versus vitronectin binding ) was explored by immunohistochemical localization of fibrinogen / fibrin and by injection of recombinant PAI 1 protein defective for vitronectin binding or with adenoviral vectors bearing a mutated binding deficient PAI 1 gene . ^^^ PAI 1 mutants defective for vitronectin binding were used to show that PAI 1 promotes choroidal pathologic angiogenesis merely through its antiproteolytic activity . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
How vitronectin binds PAI 1 to modulate fibrinolysis and cell migration . ^^^ The interaction of the plasma protein vitronectin with plasminogen activator inhibitor 1 ( PAI 1 ) is central to human health . ^^^ Vitronectin binding extends the lifetime of active PAI 1 , which controls hemostasis by inhibiting fibrinolysis and has also been implicated in angiogenesis . ^^^ The PAI 1 vitronectin binding interaction also affects cell adhesion and motility . ^^^ Here we show the crystal structure at a resolution of 2 . 3 A of the complex of the somatomedin B domain of vitronectin with PAI 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The PAI 1 binding protein vitronectin reversed the changes associated with the lack of glycosylation at one of the sites . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We hypothesized that therapy with a mutant human PAI 1 ( PAI 1R ) that binds to matrix vitronectin but does not inhibit plasminogen activators , would enhance plasmin generation , increase matrix turnover , and decrease matrix accumulation in experimental glomerulonephritis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin may control the clearance of vascular thrombi by binding and stabilizing PAI 1 , a key regulator of fibrinolysis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Protease nexin 1 ( PN 1 ) and plasminogen activator inhibitor 1 ( PAI 1 ) are serine protease inhibitors that bind to the extracellular matrix protein vitronectin ( VN ) with high affinity . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Vitronectin ( VN ) and plasminogen activator inhibitor 1 ( PAI 1 ) have important functional interactions : VN stabilises the protease inhibitory activity of PAI 1 and PAI 1 inhibits binding of adhesion receptors to VN . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
METHOD : We studied urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) , urokinase receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) expression by in situ hybridization and by immunohistochemistry in 14 NF 2 and 15 sporadic patients with 34 schwannomas . uPAR and vitronectin immunohistochemistry were also studied . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
By using PAI 1 variants and AEC from mice transgenically deficient in vitronectin ( Vn ) , we demonstrate that the PAI 1 effect requires its Vn binding activity . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In addition to inhibiting serine proteases ( mainly tPA and uPA ) , PAI 1 interacts with vitronectin ( Vn ) , fibrin or alpha ( 1 ) acid glycoprotein , interactions which are important for PAI 1 mediated effects in inflammation , tumor invasion and metastasis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 circulates in blood as a complex with vitronectin . ^^^ A PAI 1 variant ( N ( ( 2 ( iodoacetoxy ) ethyl ) N methyl ) amino 7 nitrobenz 2 oxa 3 diazole ( NBD ) P 9 PAI 1 ) with a fluorescent tag at the reactive center loop ( RCL ) was used to study the effects of vitronectin and monoclonal antibodies ( mAbs ) directed against alpha helix F ( Mab 2 and MA 55F4C12 ) on the reactions of PAI 1 with tissue type and urokinase type plasminogen activators . ^^^ Binding of vitronectin to NBD P 9 PAI 1 does not affect SI but results in a 2 . 0 6 . 5 fold decrease in the limiting rate constant ( klim ) of RCL insertion for urokinase type plasminogen activator at pH 6 . 2 8 . 0 and for tissue type plasminogen activator at pH 6 . 2 . ^^^ Binding of vitronectin to the complexes of NBD P 9 PAI 1 with mAbs results in a decrease in klim and in a 1 . 5 22 fold increase in SI . ^^^ We hypothesize that vitronectin , bound to alpha helix F on the side opposite to the epitopes of the mAbs , potentiates the mAb induced delay in RCL insertion and the associated substrate behavior by selectively decreasing the rate constant for the inhibitory branch of PAI 1 reaction ( ki ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The literature suggests that PAI 1 plays a dual role in these processes because it inhibits plasmin originated proteolysis and binds to vitronectin or integrins . ^^^ Wild PAI 1 converts quickly into its latent , inactive form and loses its anti proteolytic activity , but still binds to vitronectin and integrins . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
METHODS : The expression of uPA , tPA , urokinase receptor ( uPAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin was investigated by immunohistochemical staining , in addition to uPA , tPA and PAI 1 expression by in situ hybridization , in samples from eight chronic venous ulcers , five decubitus ulcers , five well granulating acute wounds and five normal skin samples . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
It is not always clear if these functions depend on the antiproteolytic activity of PAI 1 , on its binding to vitronectin or on its intereference with cellular migration or matrix binding . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Although PAI 1 can alter cell motility by competing for a common binding site on vitronectin , blocking this site did not alter the motility rate . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
PAI 1 also differs from other serine protease inhibitors ( serpins ) in that it is a trace protein in plasma , it has a short half life in vivo , its synthesis is highly regulated , and it binds to the adhesive glycoprotein vitronectin ( VN ) with high affinity and specificity . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) and vitronectin are cofactors involved in pathological conditions such as injury , inflammation , and cancer , during which local levels of PAI 1 are increased and the active serpin forms complexes with vitronectin . ^^^ We hypothesize that PAI 1 vitronectin complexes form in a stepwise and concentration dependent fashion via 1 : 1 and 2 : 1 intermediates , with the 2 : 1 complex serving a key role in assembly of higher order complexes . ^^^ To test this hypothesis , sedimentation velocity experiments in the analytical ultracentrifuge were performed to identify different PAI 1 vitronectin complexes . ^^^ Our results demonstrate that PAI 1 and vitronectin assemble into higher order forms via a pathway that is triggered upon saturation of the two PAI 1 binding sites of vitronectin to form the 2 : 1 complex . ^^^ This 2 : 1 PAI 1 vitronectin complex , with a sedimentation coefficient of 6 . 5 S , is the key intermediate for the assembly of higher order complexes . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
We demonstrate that PAI 1 inhibits human fibrosarcoma cell ( HT 1080 ) adhesion to vitronectin ( Vn ) via alpha ( 5 ) beta ( 5 ) integrin , and stimulates cell migration from Vn toward collagen type 4 ( Col ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) regulates the proteolytic activity of urokinase type plasminogen activator ( uPA ) and there is increasing evidence for a PAI 1 role in regulating cell migration / invasion by other mechanisms like vitronectin ( VN ) binding and lipoprotein receptor related protein ( LRP ) binding . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
OBJECTIVE : The role of vitronectin ( VN ) in thrombosis is not fully understood , primarily because this adhesive glycoprotein not only stabilizes plasminogen activator inhibitor 1 ( PAI 1 ) and thus protects fibrin from premature lysis , but also because it binds to platelet integrins and may influence platelet aggregation . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The high affinity binding site in human vitronectin ( VN ) for plasminogen activator inhibitor 1 ( PAI 1 ) has been localized to the NH ( 2 ) terminal cysteine rich somatomedin B ( SMB ) domain ( residues 1 44 ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
This positive regulation of PAI 1 on fibronectin assembly was independent of PAI 1 ' s anti proteinase activity , but acted through PAI 1 binding to the somatomedin B domain of vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In the presence of vitronectin ( Vn ) , the inhibition of thrombin and FXa by PAI 1 was further promoted by both types of heparin but to a significantly lesser extent with LMW heparin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Consequently , PAI 1 plays an important role in cardiovascular diseases ( mainly through inhibition of t PA ) and in cell migration and tumor development ( mainly through inhibition of u PA and interaction with vitronectin ) . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The functions of the serpin PAI 1 ( plasminogen activator inhibitor 1 ) are based on molecular interactions with its target proteases uPA and tPA ( urokinase type and tissue type plasminogen activator respectively ) , with vitronectin and with endocytosis receptors of the low density lipoprotein family . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Human S protein is a serum glycoprotein that binds and inhibits the activated complement complex , mediates coagulation through interaction with antithrombin 3 and plasminogen activator inhibitor 1 , and also functions as a cell adhesion protein through interactions with extracellular matrix and cell plasma membranes . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Identification of and partial characterization of platelet vitronectin : evidence for complex formation with platelet derived plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Strong staining for plasminogen activator inhibitor 1 and vitronectin was observed in chorionic trophoblasts and moderate staining in decidual connective tissue . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Sera obtained from VN deficient mice are completely deficient in `` serum spreading factor ' ' and plasminogen activator inhibitor 1 binding activities . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Along the vasculature , extracellular associated vitronectin serves as a binding and stabilizing cofactor for plasminogen activator inhibitor 1 , which thereby becomes a slow reacting thrombin inhibitor . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
In both groups 3 and 4 , these changes were significantly attenuated with intense immunostaining for plasminogen activator inhibitor 1 ; fibronectin and vitronectin were seen particularly among collagen fibers on both sides of the anastomosis . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Plasminogen activator inhibitor 1 inhibits smooth muscle cell migration and , in the presence of vitronectin , promotes the clearance of thrombin by LDL receptor related protein at sites of endothelial injury . . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Furthermore , adhesion to vitronectin and fibronectin was reduced by compounds that interfere with integrin function , such as EDTA , anti integrin antibodies , or by antibodies that interfere with the binding of pro uPA to uPAR , soluble uPAR , soluble vitronectin , phosphatidylinositol specific phospholipase C , as well as plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
The role of beta strand 5A of plasminogen activator inhibitor 1 in regulation of its latency transition and inhibitory activity by vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Activated protein C also facilitates clot lysis by inhibiting plasminogen activator inhibitor 1 , a process that is accelerated markedly by vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
Furthermore , our results suggest that hK 5 can potentially release ( a ) angiostatin 4 . 5 from plasminogen , ( b ) `` cystatin like domain 3 ' ' from low molecular weight kininogen , and ( c ) fibrinopeptide B and peptide beta 15 42 from the Bbeta chain of fibrinogen . hK 5 could also play a role in the regulation of the binding of plasminogen activator inhibitor 1 to vitronectin . ^^^
Interacting proteins: P05121 and P04004 Pubmed SVM Score :0.0
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