Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.58411462
P 14 P7 in the reactive loop of PAI 1 ) formed stable complexes with PAI 1 and converted the inhibitor to a substrate for tissue type plasminogen activator . 0.58411462^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.51584613
In addition , PAI 1 retains its capacity to form equimolar complexes with its target serine protease tissue type plasminogen activator ( t PA ) , as well as its ability to inhibit t PA activity . 0.51584613^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.70086916
We found that the quasi totality of tPA formed stable complexes with PAI 1 , thereby suppressing measurable fibrinolytic activity . 0.70086916^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.79339987
We have shown that synthetic peptides containing the amino acid sequence Asn Arg Arg Leu , derived from the amino acid sequence of the inner loop of the kringle 2 domain of tissue type plasminogen activator ( tPA ) , inhibited complex formation between two chain tPA and plasminogen activator inhibitor 1 ( PAI 1 ) by binding to PAI 1 . 0.79339987^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :1.0344412
Interactions between tPA and its main plasma inhibitor ( PAI 1 ) and between tPA and the endothelial cells lining blood vessels are two factors which may limit efficacy . 1.0344412^^^ In an attempt to identify the regions of the tPA molecule involved in these interactions , we have examined a series of synthetic peptides with amino acid sequences corresponding to different regions of the tPA molecule for their ability to protect tPA from inactivation by PAI 1 and for their ability to reduce the binding of tPA to endothelial cells . 0.62036585^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.61469803
Moreover , the interaction of PAI 1 with tPA differs from its interaction with UK , and may involve two sites on the tPA molecule . . 0.61469803^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.5487489
For comparison , a highly metastatic melanoma cell line ( Queen ' s ) that constitutively expresses high tPA production , was transfected with a cDNA coding for human plasminogen activator inhibitor type 1 ( PAI 1 ) . 0.5487489^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :1.0718617
Quantification of the complex composition between PAI 1 captured on the monoclonal antibody MAI 11 with either tPA , K2P or P gave a one to one ratio with the fraction of active PAI 1 , consistent with the results from SDS PAGE and the specific activity of PAI 1 . 1.0718617^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :1.1080466
The interactions between tPA , alpha 2 macroglobulin ( alpha 2 M ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were investigated both in vitro and in patients undergoing tPA therapy for MI in an attempt to identify variables that might affect the clinical efficacy of tPA . 1.1080466^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.53212465
AR H029953XX ( Ki = 25 microM ) prevented complex formation between tPA and active wt PAI 1 as well as that with mutants L 1 , L 2 , C 1 , C 2 , and C 4 PAI 1 . 0.53212465^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.68977416
Since the assay could easily be adapted to the assessment of the complex of tPA with its type 1 inhibitor ( PAI 1 ) , it was investigated whether the tPA : PAI 1 complex also provides prognostic information . 0.68977416^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.57969936
With these ELISAs both complexes of the activators ( uPA , tPA ) with their inhibitor ( PAI 1 ) can be measured as a separate component . 0.57969936^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.60499538
A strong association between tPA and PAI 1 was noted in the Pearson ' s correlation analysis ( p < 0 . 001 ) . 0.60499538^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.70777942
T PA binds to a specific receptor , retains its catalytic activity , and is protected from its natural inhibitor PAI 1 . 0.70777942^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.98487344
Thus , some of the molecular interactions between PAI 1 , t PA and vitronectin , previously demonstrated in purified systems in vitro , also occur in plasma . . 0.98487344^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.67985541
To identify the determinants involved in ligand binding to the receptor , several variants of t PA were assessed for their ability to form complexes with PAI 1 and thereby to inhibit specific cellular binding of complexes between structurally unmodified 125I t PA and PAI 1 . 0.67985541^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.50199114
In patients , endogenous t PA circulated predominantly as a 100 kilodalton ( kDa ) complex with PAI 1 , as verified by immunoprecipitation . 0.50199114^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.50326344
Plasma levels of t PA free PAI 1 and a complex of t PA with PAI 1 in human males and females at various ages . 0.50326344^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.80643432
The 110 , 000 band , also present in pre exercise samples , represents t PA complexed with its major inhibitor ( PAI 1 ) , and the 180 , 000 band is due to t PA complexed with C 1 inhibitor . 0.80643432^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :1.3118721
These studies are consistent with a model in which t PA binds initially to SMC associated PAI 1 with subsequent t PA . 1.3118721^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.5874183
The peptide also inhibited complex formation between PAI 1 and t PA as demonstrated by SDS PAGE analysis . 0.5874183^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.86574688
We demonstrate that fibroblasts degrade both free t PA ( PAI 1 independent ) and t PA complexed with its specific inhibitor PAI 1 ( PAI 1 dependent ) , whereas genetically altered fibroblasts that do not express LRP neither internalize nor degrade these ligands . 0.86574688^^^ One of these mechanisms involves internalization of complexes between t PA and its cognate inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) ; the other mechanism is independent of PAI 1 . 0.71438392^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , beta thromboglobulin ( beta TG ) , and fibrinopeptide A ( FPA ) were followed over 24 hours in 30 patients treated with alteplase for acute myocardial infarction . ^^^ PAI 1 antigen levels ( 55 + / 9 ng / mL at T Oh , mean + / SEM ) decreased to 35 + / 5 ( T 1 . 5h ) and 40 + / 6 ( T 2h ) ng / mL under alteplase , before increasing to 84 + / 22 ( T 3 . 5h ) , 130 + / 30 ( T 12h ) , and 64 + / 7 ( T 24h ) ng / mL after therapy , P less than . 001 . ^^^ We conclude that plasma levels of PAI 1 antigen as well as activity markedly increase after alteplase therapy of acute myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : TNK tissue plasminogen activator ( TNK TPA ) is a genetically engineered variant of TPA , which in experimental models has a slower plasma clearance and greater fibrin specificity and is 80 fold more resistant to plasminogen activator inhibitor 1 than alteplase TPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tenecteplase is a genetically engineered variant of alteplase with slower plasma clearance , better fibrin specificity , and high resistance to plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Specific mutations at three sites in the alteplase molecule result in 15 fold higher fibrin specificity , 80 fold reduced binding affinity to the physiological plasminogen activator inhibitor PAI 1 and 6 fold prolonged plasma half life ( 22 vs 3 . 5 minutes ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tenecteplase is a triple combination mutant variant of alteplase with high fibrin specificity and resistance to plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of tissue type plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , and t PA / PAI 1 complex antigens were analyzed in the plasma of disseminated intravascular coagulation ( DIC ) patients and healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HTC plasminogen activator inhibitor type 1 ( PAI 1 ) production was stimulated by dexamethasone , serum factors and insulin ; that of tissue type plasminogen activator ( tPA ) by cAMP raising agents . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic response of type 4 patients was comparable to that of normolipidemic subjects , whereas tissue type plasminogen activator antigen levels before and after venous occlusion ( p less than 0 . 01 ) and resting plasminogen activator inhibitor 1 ( PAI 1 ) antigen ( p less than 0 . 01 ) and activity ( p less than 0 . 01 ) were significantly higher in hypertriglyceridemic subjects compared with controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have shown that plasminogen activator inhibitor 1 ( PAI 1 ) inhibits the fibrin binding of both the single chain and two chain forms of tissue type plasminogen activator ( tPA ) through two different mechanisms . ^^^ Interactions between the finger and kringle 2 domains of tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we report the effects of recombinant human TNF alpha on the synthesis of tissue type plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) by human mesangial cells in culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The releases of tissue type plasminogen activator ( t PA ) , and epoprostenol ( Epo ) from endothelial cells were stimulated by NB , but no significant effect of plasminogen activator inhibitor ( PAI ) activity was seen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Receptor mediated endocytosis of tissue type plasminogen activator ( t PA ) plasminogen activator inhibitor type 1 ( PAI 1 ) complexes results in their clearance by Hep G 2 cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Two plasminogen activators ( PAs ) : tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , as well as the type 1 plasminogen activator inhibitor ( PAI 1 ) are synthesized and secreted by rat astrocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Two recent findings , however , strongly suggest a contribution : ( 1 ) Increased lipoprotein ( a ) levels that reduce tissue type plasminogen activator ( t PA ) mediated clot lysis are a clear risk factor for atherosclerosis ; and ( 2 ) increased plasminogen activator inhibitor 1 ( PAI 1 ) levels in patients with disturbed glucose tolerance predispose to an accelerated development of atherosclerotic disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Expression of plasminogen activator and plasminogen activator inhibitor mRNA in human fibroblasts grown on different substrates . mRNA levels for urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) were examined in human diploid ( neonatal foreskin ) fibroblasts grown in 200 ml microcarrier suspension culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 was blocked by natural and recombinant alpha 2MRAP , and about 80 % inhibited by complexes between tissue type plasminogen activator ( tPA ) and PAI 1 , and by a monoclonal anti PAI 1 antibody . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These complexes were probably formed by activation of urokinase type plasminogen activator ( u PA ) , and not tissue type plasminogen activator ( t PA ) , since SF levels of both u PA antigen and u PA plasminogen activator inhibitor ( PAI ) complexes were increased in 27 of the 42 patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Retinoic acid induces tissue type plasminogen activator ( t PA ) but not plasminogen activator inhibitor 1 ( PAI 1 ) expression in cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the salivary samples the concentrations of tPA ( tissue type plasminogen activator ) , PAI 1 ( plasminogen activator inhibitor type 1 ) , albumin and total protein were determined and the activity of amylase , tPA and PAI assessed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunological and functional levels of tissue type plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) were quantified in platelet poor plasma ( PPP ) from patients with CTEPH as well as age matched controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In RA patients , extravascular t PA mediated plasminogen activation was depressed : mean SF tissue type plasminogen activator ( t PA : Ag ) concentration ( 2 . 1 + / 1 . 6 ng / ml ) was four fold lower , and plasminogen activator inhibitor ( PAI ) activity ( 284 + / 212 % ) four fold higher than the plasma values of the same patients or of healthy donors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator antigen ( t PA : Ag ) , urokinase type plasminogen activator antigen ( u PA : Ag ) , the proenzyme single chain u PA ( scu PA ) , and plasminogen activator inhibitor ( PAI ) were measured in the synovial fluid and plasma of 22 patients with seropositive rheumatoid arthritis ( RA ) , 13 with seronegative RA , and 23 patients with various forms of arthritis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentration of thrombin antithrombin 3 complex ( TAT ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , PAI 2 , D dimer complex and urokinase plasminogen activator ( u PA ) activity were studied in 30 patients with acute nonlymphoblastic leukemia ( ANLL ) , before and during antileukemic therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , it was demonstrated that ( 1 ) theca interstitial compartment synthesizes the majority of PAI 1 activity in the ovary before ovulation , the follicular wall may therefore serve as a specific barrier to prevent the secretion of PA into the extrafollicular compartment ; ( 2 ) Granulosa cells contribute only small amount of ovarian PAI 1 activity , but synthesize most of tissue type plasminogen activator activity involved in the process leading to ovulation : ( 3 ) Since only matured cumulus oocyte complexes secrete high level of tPA and PAI 1 , both tPA and PAI 1 activity in the conditioned medium may be used as reliable markers for evaluating oocyte quality for in vitro fertilization . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The level of functional PAI 1 , tissue type plasminogen activator ( t PA ) activity and t PA PAI 1 complex was measured in normal subjects and in patients with venous thromboembolism in a silent phase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before occlusion plasma levels of tissue type plasminogen activator ( t PA ) antigen and its inhibitor ( PAI 1 ) were significantly higher in the patient group ( p < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Inactivation of tissue type plasminogen activator ( tPA ) by PAI 1 was slowed 10 fold in the presence of peptides derived from the tPA finger and kringle 2 domains . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activators ( PA ) , tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , alpha 2 antiplasmin ( alpha 2 AP ) , plasminogen activator inhibitor ( PAI ) , fibrinogen / fibrin degradation products ( FDP ) and D dimer were determined to elucidate the role of plasminogen activators and inhibitors in the pathogenesis of accelerated fibrinolysis in schistosomiasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) is the primary inhibitor of tissue type plasminogen activator and has been found to be increased in a number of clinical conditions generally defined as prothrombotic . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A rabbit ear model of blood loss was developed to compare the effects of an active form of recombinant plasminogen activator inhibitor 1 ( rPAI 1 ) with epsilon amino caproic acid ( EACA ) in antagonizing tissue type plasminogen activator ( r tPA ) induced blood loss . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both treatments induced a transient phase of increased blood fibrinolytic activity , as evidenced by higher levels of tissue type plasminogen activator ( t PA ) activity and decreased levels of PA inhibitor ( PAI ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and the t PA / PAI 1 complex ( including various coagulation factors ) were assayed in 72 patients with coronary artery disease ( CAD ) and 29 control ( C ) subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Whilst increased plasma beta thromboglobulin ( beta TG ) , platelet factor 4 and thrombospondin levels are regarded as reflecting the release reaction of platelets , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) levels represent endothelial release reaction and / or damage . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have previously shown that alpha thrombin exerted a mitogenic effect on human glomerular epithelial cells and stimulated the synthesis of urokinase type ( u PA ) and tissue type plasminogen activator ( t PA ) and of their inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We observed no significant differences between diets in terms of fasting plasma levels of factor 7 coagulant activity , fibrinogen , euglobulin fibrinolytic activity , tissue type plasminogen activator ( t PA ) activity , t PA antigen , plasminogen activator inhibitor type 1 ( PAI 1 ) antigen , or PAI activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human mesangial cells in culture synthesize and secrete plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) . ^^^ Cell specific regulation of plasminogen activator inhibitor 1 and tissue type plasminogen activator release by human kidney mesangial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a specific inhibitor of the serine proteases tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a specific inhibitor of the serine proteases tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate whether baseline levels of endogenous tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) predict the future occurrence of venous thrombosis , levels of these proteins were measured in prospectively collected plasma samples from 55 participants in the Physicians ' Health Study who later developed deep venous thrombosis or pulmonary embolism and from an equal number of age and smoking matched control subjects who remained free of vascular disease during a mean follow up period of 60 . 2 months . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of tissue type plasminogen activator ( t PA ) in plasma , is a serine proteinase inhibitor ( serpin ) that forms a 1 : 1 stoichiometric complex with its target proteinase leading to the formation of a stable inactive complex . ^^^ Identification of a conformationally distinct form of plasminogen activator inhibitor 1 , acting as a noninhibitory substrate for tissue type plasminogen activator . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of tissue type plasminogen activator ( t PA ) in plasma , is a serine proteinase inhibitor ( serpin ) that forms a 1 : 1 stoichiometric complex with its target proteinase leading to the formation of a stable inactive complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Occasional macrophage like cells also stained for urokinase but tissue type plasminogen activator and plasminogen activator inhibitor 1 were restricted to vascular endothelium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) induces synthesis of a rapidly acting inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) in human umbilical vein endothelial cells ( HUVEC ) in culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The cause must be searched in the observed increase of plasminogen activator inhibitor ( PAI ) likly in combination with a decrease of the tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to compare plasma levels of urokinase type plasminogen activator ( u PA ) , before and after 20 min of venous stasis , with those of tissue type plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) and t PA / PAI 1 complexes , to determine whether both plasminogen activators and their inhibitor respond similarly to the same stimulus . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The patients had a lower mean fibrinolytic response to venous occlusion than the controls and higher antigen levels of tissue type plasminogen activator ( t PA : Ag ) and plasminogen activator inhibitor type 1 ( PAI 1 : Ag ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To examine the fibrinolytic capacity in patients with acute myocardial infarction ( AMI ) , baseline levels of plasma plasminogen activator inhibitor ( PAI ) activity and tissue type plasminogen activator ( t PA ) antigen were measured in 47 patients with Q wave AMI who underwent emergent coronary angiography 3 . 0 + / 0 . 2 hours after the symptom onset . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human mesangial cells secrete tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , the latter being secreted in large excess in vitro . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine the relationship between cardiovascular complications of estrogen therapy and fibrinolysis , fibrinolysis parameters plasminogen , urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , were assessed in 12 prostatic cancer patients before and 6 weeks after the onset of estrogen therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Role of protein kinase C and cyclic adenosine monophosphate in the regulation of tissue type plasminogen activator , plasminogen activator inhibitor 1 , and platelet derived growth factor mRNA levels in human endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of tissue type plasminogen activator , has been shown to be an independent risk factor for recurrent myocardial infarction ( MI ) at a young age . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of tissue type plasminogen activator , has been shown to be an independent risk factor for recurrent myocardial infarction ( MI ) at a young age . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the fast acting inhibitor of tissue type plasminogen activator ( t PA ) and urokinase ( u PA ) , is a member of the serpin superfamily of proteins . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
By enzyme linked immunosorbent assays , forskolin was found to cause a 2 to 4 fold decrease in intracellular and culture medium levels of type 1 inhibitor of plasminogen activators ( PAI 1 ) and tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 6 patients undergoing programmed ventricular stimulation with repeated induction of clinical ventricular tachycardia , the coronary release of tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI ) capacity , von Willebrand factor antigen ( WF : Ag ) , and prostacyclin ( 6 keto PGF 1a ) was measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased plasminogen activator inhibitor 1 ( PAI 1 ) antigen levels in plasma from patients with septicemia decreased the ratio of the plasma clot lysis rate induced by an anti alpha 2 plasmin inhibitor monoclonal antibody to the tissue type plasminogen activator ( t PA ) concentration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) regulates fibrinolysis by inhibiting tissue type plasminogen activator ( t PA ) . ^^^ The inhibition of tissue type plasminogen activator by plasminogen activator inhibitor 1 . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) regulates fibrinolysis by inhibiting tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vitronectin governs the interaction between plasminogen activator inhibitor 1 and tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The acute phase behaviour of the fast inhibitor of tissue type plasminogen activator ( PAI 1 ) in vivo has been attributed to increased synthesis by endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antigen levels of both tissue type plasminogen activator and plasminogen activator inhibitor 1 were increased in mild and severe cirrhosis . ^^^ In most patients , the increase in tissue type plasminogen activator was counterbalanced by the increased levels of plasminogen activator inhibitor 1 , but in a subgroup of patients the change in balance resulted in extremely high tissue type plasminogen activator levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , it did not significantly modulate urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , and tissue factor ( TF ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vitronectin endows plasminogen activator inhibitor 1 ( PAI 1 ) , the fast acting inhibitor of both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , with additional thrombin inhibitory properties . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A mutant of recombinant tissue type plasminogen activator ( rt PA ) , obtained by deletion of residues Lys 296 to Gly 302 [ rt PA del ( K 296 G302 ) ] , was previously shown to be resistant to inhibition by plasminogen activator inhibitor 1 ( PAI 1 ) ( Madison et al , Nature 339 : 721 , 1989 ) . ^^^ Biochemical and biologic properties of rt PA del ( K 296 G302 ) , a recombinant human tissue type plasminogen activator deletion mutant resistant to plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , and PAI 1 activity were determined in 1 . 25 ml of conditioned medium and t PA and PAI 1 mRNA in the cell extracts of 2 10 10 ( 6 ) HUVE cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured antigen levels of 2 kinds of plasminogen activator , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( UK ) , as well as those of their primary inhibitors , type 1 plasminogen activator inhibitor ( PAI 1 ) and type 2 plasminogen activator inhibitor ( PAI 2 ) , in tissue extracts from benign and malignant breast tumors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have reported previously that incubation of HTC rat hepatoma cells with the synthetic glucocorticoid dexamethasone causes a 90 % decrease in tissue type plasminogen activator ( tPA ) activity secondary to a 4 fold increase in plasminogen activator inhibitor 1 ( PAI 1 ) mRNA accumulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endotoxin treated rabbits produce high levels of plasminogen activator inhibitor 1 ( PAI 1 ) , which inhibits fibrinolysis by neutralizing endogenous tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coagulation studies indicated that both patients acquired high levels of tissue type plasminogen activator inhibitor ( PAI ) with deficiency in tissue type plasminogen activator ( t PA ) and consequent impaired fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recent studies have established that , of the known components of the fibrinolytic system , only tissue type plasminogen activator ( tPA ) and its fast acting inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , show a marked circadian variation in plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied the diurnal variation in tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) antigens and activities during a 24 h period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate short term activation and inhibition of fibrinolysis during shock , we studied plasma levels of tissue type plasminogen activator ( t PA ) and t PA inhibition capacity ( PAI ) in anaesthetized pigs . t PA in euglobulin fractions of plasma was measured by the conversion of plasminogen to plasmin in the presence of fibrin split products . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Univariate statistical analysis showed Lp ( a ) concentration to be associated with fibrinogen concentrations in both sexes ( m : r = 0 . 08 , P less than 0 . 05 ; f : r = 0 . 20 , P less than 0 . 001 ) , but not with euglobulin fibrinolysis activity , tissue type plasminogen activator , plasminogen activator inhibitor type 1 ( PAI 1 ) , or the split products of cross linked fibrin ( d dimer ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A decreased PAI expressed against tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) was found in lungs of all treated rats compared to controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human glomerular epithelial cells ( GECs ) in culture synthesize single chain , urokinase type plasminogen activator ( SC uPA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) and possess specific membrane binding sites for u PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 is a physiological inhibitor of both tissue type plasminogen activator and urokinase type plasminogen activator , key enzymes in the initiation of fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) activity and antigen increased significantly in normal subjects , while plasminogen activator inhibitor ( PAI ) activity decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of recombinant tissue type plasminogen activator ( rt PA ) and of an inactive mutant of rt PA , obtained by mutagenesis of the active site Ser 478 to Ala ( rt PA Ala 478 ) , on the synthesis and secretion of plasminogen activator inhibitor 1 ( PAI 1 ) by human umbilical vein endothelial cells ( HUVEC ) in culture were studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Consistently , however , over a period of 7 years , a high basal euglobulin fibrinolytic activity was observed that was characterized by a high tissue type plasminogen activator ( t PA ) activity , normal t PA antigen , and undetectable plasminogen activator inhibitor type 1 ( PAI 1 ) antigen and activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the reversible interaction of plasminogen activator inhibitor 1 with tissue type plasminogen activator and with urokinase type plasminogen activator . ^^^ The second order association rate constant ( k 1 ) of complex formation of recombinant two chain tissue type plasminogen activator ( rt PA ) or recombinant two chain urokinase type plasminogen activator ( rtcu PA ) by recombinant plasminogen activator inhibitor 1 ( rPAI 1 ) is 2 . 9 + / 0 . 4 10 10 ( 7 ) M 1 s 1 ( mean + / S . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of 6 months of intensive endurance exercise training on resting tissue type plasminogen activator ( t PA ) activity , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , t PA antigen , and fibrinogen were studied in 10 young ( 24 30 years ) and in 13 old male subjects ( 60 82 years ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) and euglobulin lysis time ( ELT ) were determined before and after the venous occlusion test ( VOT ) in 3 groups of patients with mean age about 60 years : 29 diabetic patients ( D group ) , 8 hyperlipidemic patients ( H group ) and 19 healthy controls ( C group ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
EC have been demonstrated to release tissue type plasminogen activator ( t PA ) and its rapid inhibitor type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) antigen , tissue type plasminogen activator ( t PA ) antigen and activity , plasminogen activator inhibitor ( PAI ) type 1 antigen , PAI activity , antithrombin ( AT ) 3 activity , and protein C activity were measured in 24 patients suffering from sepsis or septic shock and the results were compared with those observed in 30 non sepsis patients with severe infectious disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous nucleotide ( nt ) sequence analysis of the 5 ' flanking DNA of the gene ( PAI 1 ) encoding plasminogen activator inhibitor 1 revealed an extensive region of shared nt sequence identity with the 5 ' flanking region of the gene ( t PA ) encoding tissue type plasminogen activator [ Bosma et al . , J . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the specific , fast acting inhibitor of tissue type plasminogen activator ( t PA ) , binds to fibrin and has been found in high concentrations within arterial thrombi . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the specific , fast acting inhibitor of tissue type plasminogen activator ( t PA ) , binds to fibrin and has been found in high concentrations within arterial thrombi . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin fibrinolytic activity ( EFA ) , tissue type plasminogen activator ( t PA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) activity , plasminogen , alpha 2 antiplasmin ( alpha 2 AP ) , fibrinogen degradation products ( FDP ) , and D dimer were measured pre , intra , and postoperatively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , play an important role in regulating the fibrinolytic capacity of plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) and other substances of coagulation fibrinolysis , such as fibronectin ( Fn ) and von willebrand factor ( vWF ) as well as the activity content of antithrombin 3 ( AT 3 ) in plasma were determined in 20 patients with acute myocardial infarction ( AMI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We determined plasma levels of tissue type plasminogen activator ( t PA ) antigen , urokinase type plasminogen activator ( u PA ) antigen , and activity of the fast acting inhibitor of plasminogen activator ( PAI 1 ) in patients with different stages of liver cirrhosis ( Child A , B , and C ) and in age and sex matched healthy controls to investigate the contribution of the liver to the metabolism of these main components of the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activator ( PA ) and PAI activity were unaltered by administration of PAI 1 containing clots , and the clot protective effects of clot bound PAI 1 were reversed by exogenous tissue type plasminogen activator administration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since disseminated intravascular coagulation ( DIC ) may directly reflect the abnormal regulation of the fibrinolytic system by endothelial cells , we have measured the levels of tissue type plasminogen activator ( t PA ) , type 1 PA inhibitor ( PAI 1 ) and t PA . ^^^ Levels of tissue type plasminogen activator and plasminogen activator inhibitor 1 in disseminated intravascular coagulation syndrome ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HUVEC responded to exposure to 42 degrees C with a time dependent increase in plasminogen activator inhibitor type 1 ( PAI 1 ) activity and antigen accompanied by a four to fivefold increase in PAI 1 specific m RNA and a decrease in tissue type plasminogen activator ( t PA ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study evaluates the effect of retinoic acid on the synthesis of tissue type plasminogen activator ( t PA ) and of plasminogen activator inhibitor 1 ( PAI 1 ) by cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^ Effect of retinoic acid on the synthesis of tissue type plasminogen activator and plasminogen activator inhibitor 1 in human endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The extracellular localizations of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were examined in cultured bovine capillary endothelial cells ( BCEs ) by an immunofluorescence method using BCEs treated with or without saponin and focal contact preparations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , when recanalization of completely occluded vessels was induced with tissue type plasminogen activator ( t PA ) , plasma PAI 1 increased more markedly ( from 5 . 6 + / 0 . 7 to 112 . 8 + / 22 . 3 AU / ml , n = 11 ) , exceeding the increase after corresponding intervals in animals in which t PA failed to induce recanalization ( from 5 . 2 + / 1 . 1 to 28 . 3 + / 6 . 1 AU / ml , n = 6 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured antigen levels of two kinds of plasminogen activators , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( UK ) , as well as their primary inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) in the tissue extracts of benign and malignant breast tumors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
High levels of von Willebrand factor ( vWF ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) antigens were also recorded in some patients with systemic bleeding with or without incoagulable blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunoabsorption of the rabbit anti PAI 1 with purified PAI 1 , or treatment of HUVECs with tissue type plasminogen activator ( 2 . 5 micrograms / ml , 2 h , 4 degrees C ) reduced the amount of staining both on the apical surface and in the extracellular matrix of agonist activated HUVECs by 80 95 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
IX , von Willebrand Factor , protein C , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , beta thromboglobulin , platelet factor 4 , fibrinopeptide A , plasmin alpha 2 antiplasmin complex and FDP . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The interactions between exogenously added tissue type plasminogen activator ( t PA ) and the active form of type 1 plasminogen activator inhibitor ( PAI 1 ) produced by and present in cultured human umbilical vein endothelial cells ( HUVECs ) were investigated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The formation and release of covalent complexes between tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) limits the application of equilibrium radioligand binding analysis to characterize the interaction between t PA and human umbilical vein endothelial cell ( HUVEC ) monolayers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to investigate this question , we studied human renal biopsies by immunofluorescence technique with specific antibodies for fibrin , tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) , PAI 1 and PAI 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The amount of antigen of the two fibrinolytic factors , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , was determined using ELISA and the ABC method , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We report here that human omental tissue mesothelial cells in vitro produce large amounts of tissue type plasminogen activator ( t PA ) , together with type 1 and 2 plasminogen activator inhibitor ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis is regulated in part by the interaction between tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 , a serine protease inhibitor of the serpin family ) . ^^^ Amino acid residues that affect interaction of tissue type plasminogen activator with plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of IGF 1 on the secretion of plasminogen activators was assessed using an immunological assay for tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Structural determinants of the noncatalytic chain of tissue type plasminogen activator that modulate its association rate with plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The influence of diacylglycerols , which are physiological activators of protein kinase C , on the production of tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) by human umbilical vein endothelial cells ( HUVEC ) was studied in order to gain insight into the regulation of fibrinolysis by these cells . 1 , 2 dioctanoyl sn glycerol ( diC 8 ) stimulated tPA production in a dose and time dependent manner . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Short term effects of moderate alcohol consumption on platelet function , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) were studied in two age groups of volunteers ( 20 30 and 45 55 years ) , each consisting of eight healthy males . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of anoxia and reoxygenation on the synthesis and secretion of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) was studied in primary cultures of human umbilical vein endothelial cells . ^^^ Oxygen radicals generated during anoxia followed by reoxygenation reduce the synthesis of tissue type plasminogen activator and plasminogen activator inhibitor 1 in human endothelial cell culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) and fibrinopeptide A ( FPA ) levels were measured in the great cardiac venous and arterial blood of 9 patients with clinically and angiographically proven vasospastic angina and 11 controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We report here the effect of purified human alpha thrombin on the synthesis of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) by cultured human mesangial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in plasminogen activator inhibitor 1 and tissue type plasminogen activator during exercise in patients with coronary artery disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator and its inhibitor ( PAI 1 ) in plasma in cases of non insulin dependent diabetes mellitus ( NIDDM ) ] . ^^^ Parameters of fibrinolysis , including plasminogen , alpha 2 plasmin inhibitor ( alpha 2 PI ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigens , and fibrinogen were assayed in 53 patients ( 28 women and 27 men ; mean age : 64 years , age range : 32 87 years ) with non insulin dependent diabetes mellitus ( NIDDM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was performed to determine whether pharmacologic concentrations of tissue type plasminogen activator ( t PA ) can initiate negative feedback by stimulating PAI 1 synthesis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The cytokines tumor necrosis factor ( TNF ) , lymphotoxin , and interleukin 1 ( IL 1 ) , but not interleukin 6 , increase the production of plasminogen activator inhibitor 1 ( PAI 1 ) by endothelial cells , whereas they have no stimulatory effect on the production of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HTC rat hepatoma cells synthesize and secrete tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human non small lung cancer cell lines HS 24 ( established from a primary squamous cell carcinoma ) and SB 3 ( established from a metastasis of a primary adenocarcinoma of the lung into the adrenal gland ) were analysed for the proteinases tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have reported that glucocorticoids and cyclic nucleotides regulate tissue type plasminogen activator activity in HTC rat hepatoma cells primarily by modulating plasminogen activator inhibitor ( PAI 1 ) gene expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There was a markedly significant increase of plasma levels of tissue type plasminogen activator , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI activity in 3 patients with hemophagocytic histiocytosis ( HPH ) as compared with DIC patients with multi organ failure ( MOF ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MA 7D4 was used previously as capture antibody in combination with MA 7F5 conjugated to horseradish peroxidase ( 7F5 HRP ) to construct an ELISA which was 12 times more sensitive towards free PAI 1 as compared to PAI 1 in complex with human tissue type plasminogen activator ( t PA ) ( Blood 71 : 220 225 , 1988 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to assess the actual degree of activation of the coagulation and fibrinolytic systems in diabetics , plasma levels of thrombin antithrombin 3 complex ( TAT ) and plasmin alpha 2 plasmin inhibitor complex ( PAP ) were measured together with tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) in 18 patients with DM ( three patients with type 1 DM and 15 with type 2 DM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate fibrinolytic activity , we measured plasma concentrations of tissue type plasminogen activator , plasminogen activator inhibitor 1 , and cross linked fibrin degradation products . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The only one found in the plasma of nonpregnant women ( PAI 1 ) is immunologically related to a PAI of endothelial cells ; its plasma activity , as deduced from the inhibition of single chain tissue type plasminogen activator ( t PA ) , increased from 3 . 4 + / 2 . 3 U / mL ( mean + / 95 % confidence limits ) in the plasma of nonpregnant women to 29 + / 7 U / mL at term , and its antigen level , measured by a radioimmunoassay , increased from 54 + / 17 ng / mL to 144 + / 25 ng / mL . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 BP complex was further purified by immunoadsorption on an immobilized murine monoclonal antibody directed against PAI 1 ( MA 7D4 ) and by elution with 4 M KSCN . 4 ) The complex was then dissociated by addition of excess human tissue type plasminogen activator ( t PA ) , and t PA and PAI 1 antigen ( t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The purified inhibitor ( Mr 47 , 000 ) reacts with tissue type plasminogen activator ( t PA ) , urokinase ( UK ) , and activated protein C ( APC ) to yield both SDS stable complexes and a modified PAI of slightly reduced molecular weight . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand , important modifications of fibrinolytic parameters occurred , essentially concerning tissue type plasminogen activator ( t PA ) and its specific inhibitor ( PAI ) . t PA activity constantly increased in the course of transplantation , reaching a maximum at the end of anhepaty . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentrations of tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 were studied in 53 patients with liver deficiency caused by chronic alcoholism ( n = 40 ) , viral hepatitis ( n = 10 ) or malignant disease of the liver ( n = 3 ) and compared to that of a control group ( n = 20 ) of healthy subjects . u PA and PAI 1 levels were significantly increased in all patients with chronic alcoholism , whereas high t PA was only observed in combination with disturbed liver function tests or with liver cirrhosis ( two and six fold above control values , respectively ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) was identified in extracts of rat adrenal medulla , and its immunohistochemical localization was studied together with that of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To correlate PAI levels to patency of infarct arteries after thrombolytic therapy with recombinant tissue type plasminogen activator ( rt PA ) , 125 consecutive patients with AMI were examined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) and , therefore , is an important regulator of plasminogen activation . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) and , therefore , is an important regulator of plasminogen activation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activity and antigen , tissue type plasminogen activator ( tPA ) activity and antigen , plasminogen activator inhibitor ( PAI ) activity , and plasmin generation rates were determined in 32 normal newborn plasmas and 25 normal adult plasmas . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Trace amounts of tissue type plasminogen activator ( t PA ) complexed to a plasminogen activator inhibitor type 1 ( PAI 1 ) were identified with specific antibodies . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We observed a significant change in the activity of tissue type plasminogen activator ( t PA ; p less than 0 . 01 ) and in the activity of plasminogen activator inhibitor ( PAI ; p less than 0 . 001 ) during 24 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentrations of urokinase plasminogen activator ( u PA / competitive radioimmunoassay ) , tissue type plasminogen activator ( t PA / sandwich ELISA ) and plasminogen activator inhibitor ( PAI / functional assay ) were determined in 44 women ( age : 24 . 3 + / 4 . 3 years ) with normal pregnancy near term . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In plasma of IBD patients significantly decreased tissue type plasminogen activator activity ( t PA ) ( p less than 0 . 02 ) , increased plasminogen activator inhibition ( PAI ) ( p less than 0 . 01 ) and fibrinogen ( p less than 0 . 001 ) , and prolonged thrombin time ( p less than 0 . 001 ) and prothrombintime ( p less than 0 . 001 ) were found . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of tissue type plasminogen activator and thus performs an essential role in the regulation of the fibrinolytic process . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of tissue type plasminogen activator and thus performs an essential role in the regulation of the fibrinolytic process . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PA 1 inhibited the tissue type plasminogen activator , and also that of the two chain form of urokinase , but not the one chain form . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Expression of PAI 1 mRNA was inversely related to the distribution of tissue type plasminogen activator mRNA ( 2 . 3 kilobases ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both the urokinase type and tissue type plasminogen activator can convert their approximately 54 kDa type 1 inhibitor ( PAI 1 ) to an inactive form with a lower apparent molecular mass . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PA 1 migrates as a closely spaced doublet of 47 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis and forms covalent complexes with urokinase and two chain tissue type plasminogen activator , displaying second order rate constants of 0 . 9 10 10 ( 6 ) M 1 s 1 and 0 . 2 10 10 ( 6 ) M 1 s 1 , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Conditioned media collected from IL 1 treated ( 5 U / ml , 24 h ) monolayers exhibited decreased tissue type plasminogen activator ( tPA ) activity and increased plasminogen activator inhibitor ( PAI ) activity , as assessed by fibrin and reverse fibrin autography . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The mechanism by which activated protein C stimulates fibrinolysis was studied in a simple radiolabeled clot lysis assay system containing purified tissue type plasminogen activator , bovine endothelial plasminogen activator inhibitor ( PAI ) , plasminogen , 125I fibrinogen and thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The secretion of tissue type plasminogen activator antigen ( t PA Ag ) and fast acting plasminogen activator inhibitor activity ( PA 1 activity ) was measured in the conditioned medium after incubation of confluent cultures with serum free medium for 24 hours ( 1 . 5 ml / 25 cm 2 cell surface ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Most PAI 1 in the ECM formed complexes with exogenously added tissue type plasminogen activator ( tPA ) , demonstrating that this PAI 1 was functionally active . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A radioimmunoassay was developed that measures both free and tissue type plasminogen activator ( t PA ) bound PAI 1 in plasma and has an effective range of 8 to 250 ng / mL . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the presence of PS vesicles , PAI 1 inhibited tissue type plasminogen activator 50 fold more effectively than in the absence of phospholipids , whereas sodium dodecyl sulfate enhanced PAI 1 activity by 25 fold . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , the addition of increasing concentrations of tissue type plasminogen activator ( t PA ) or urokinase to confluent endothelial cells produced a saturable ( 3 . 0 pmol / 5 10 10 ( 5 ) cells ) , dose dependent increase of the activator PAI 1 complex in the conditioned medium even in the presence of actinomycin D or cycloheximide . ^^^ Interaction of tissue type plasminogen activator and plasminogen activator inhibitor 1 on the surface of endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular or tissue type plasminogen activator ( plasma t PA ) is the circulating physiological fibrinolytic enzyme of endothelial cell origin which function is regulated by fibrin and a specific inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present work was to clarify to what extent plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) contribute to the increase in plasma inhibition of tissue type plasminogen activator ( t PA ) observed during pregnancy . ^^^ Plasminogen activator inhibitor 1 is the primary inhibitor of tissue type plasminogen activator in pregnancy plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Constitutive gene expression of four components of plasminogen activating enzyme system , urinary and tissue type plasminogen activator ( u PA and t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 in HT 1080 human fibrosarcoma cells , was modulated by the synthetic glucocorticoid dexamethasone ( Dex , 10 ( 7 ) M ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Reference values of tissue type plasminogen activator ( t PA ) antigen , t PA activity , and plasminogen activator inhibitor ( PAI ) were established in healthy blood donors using different assay systems . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
During a survey of four month ' s duration the following parameters were determined in 43 healthy blood donors ( 22 males , 21 females ; mean age 29 years / 20 49 / ) : plasminogen activity , plasminogen concentration , alpha 2 antiplasmin ( alpha 2 AP ) activity , alpha 2 AP concentration , tissue type plasminogen activator ( t PA ) activity , t PA concentration , plasminogen activator inhibitor 1 ( PAI 1 ) activity , AT 3 activity , AT 3 concentration and heparin cofactor 2 ( HC 2 ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Incubation of HTC rat hepatoma cells with the synthetic glucocorticoid dexamethasone rapidly inhibits tissue type plasminogen activator activity by inducing a specific plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We used specific , sensitive assays for plasma tissue type plasminogen activator ( t PA ) antigen , t PA activity , plasminogen activator inhibitor ( PAI ) activity , plasminogen , and alpha 2 plasmin inhibitor ( alpha 2 PI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Functional and immunologic assays indicated that both cytokines decreased HUVEC tissue type plasminogen activator ( tPA ) and increased type 1 plasminogen activator inhibitor ( PAI 1 ) in a dose and time dependent manner . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using a solid phase radioimmunoassay , we observed that antibodies to recombinant tissue type plasminogen activator ( rt PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) bound to the surface of cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^ These studies suggest that tissue type plasminogen activator and PAI 1 are expressed on the surface of cultured HUVEC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A major finding is the identification of a large region of shared nucleotides ( comprising of about 520 bp ) between the 5 ' flanking DNAs of PAI 1 and tissue type plasminogen activator genes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Daytime fluctuations in blood of tissue type plasminogen activator ( t PA ) and its fast acting inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood was collected at least 3 months after the last acute episode , and PAI 1 antigen and activity , as well as tissue type plasminogen activator ( t PA ) antigen , urokinase type plasminogen activator ( u PA ) antigen , and fibrinolytic activity were measured in these samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human rTNF / Cachectin was shown to stimulate gene transcription of plasminogen activator inhibitor ( PA 1 ) 1 and PAI 2 , and simultaneously suppress constitutive gene expression of tissue type plasminogen activator ( t PA ) in human fibrosarcoma cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The granulosa cells of 49 follicles from 20 patients undergoing in vitro fertilization were obtained by laparoscopy and tested for the content of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and inhibitor of plasminogen activator ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The vascular endothelium plays an important role in fibrinolysis by producing tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma PAI activity and tissue type plasminogen activator ( t PA ) concentrations were measured in 61 patients with human septicaemia and results were compared with those observed in healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following tests were performed : euglobulin clot lysis time ( ECLT ) , fibrinolytic activity of euglobulins on fibrin plates in the presence and absence of blocking antibodies to tissue type plasminogen activator ( t PA ) and / or urokinase ( u PA ) , overall plasminogen activator inhibitor ( PAI ) activity , antigen levels of t PA , u PA and PAI 1 and zymography of the euglobulin fraction after SDS PAGE . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The assay is 12 times less sensitive toward the PAI 1 / human tissue type plasminogen activator ( t PA ) complex as compared with free PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These assays are based upon the binding of PAI 1 and PAI 2 to immobilized single chain tissue type plasminogen activator ( tPA ) and to immobilized urokinase ( UK ) , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
SDS gel electrophoresis of the isolated high Mr fraction revealed several bands including a main 46 , 000 Mr component , which reacted with anti ( PAI 1 ) antibodies on immunoblotting and neutralized tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the rat , gonadotropins appear to increase granulosa cell tissue type plasminogen activator ( t PA ) content by increasing the cellular concentration of t PA mRNA as well as by modulating the activity of a specific PA inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 inhibits both tissue type plasminogen activator and urokinase type plasminogen activator and may therefore be implicated in the control of various physiological processes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PA 1 measured in cell extracts was SDS resistant with an apparent m . w . of 53 Kd and inhibited both tissue type plasminogen activator ( t PA ) and urokinase ( UK ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This assay was based on the ability of the PAI to bind rapidly and strongly to immobilized tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in PIC , tissue type plasminogen activator , and plasminogen activator inhibitor 1 ( PAI 1 ) in the clinical course of case 1 suggested that fibrinolysis was suppressed by relatively elevated level of PAI 1 around the operation , but thereafter was adversely accelerated by relatively lower levels of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined PAI activity , PAI 1 antigen , tissue type plasminogen activator ( t PA ) activity , t PA antigen , plasmin alpha 2 antiplasmin complex ( PAP complex ) and fibrin degradation products D dimer in 62 patients before ( unstimulated ) and after infusion of 1 desamino 8 D arginine vasopressin ( DDAVP ; stimulated ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase binding and catabolism by Hep G 2 cells is plasminogen activator inhibitor 1 dependent , analogous to interactions of tissue type plasminogen activator with these cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The profiles of the coagulation and fibrinolytic systems were monitored by measuring several parameters , including plasma levels of thrombin antithrombin 3 complex , antithrombin 3 , plasmin alpha 2 plasmin inhibitor complex , fibrin degradation product D dimer , tissue type plasminogen activator , and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma thrombomodulin , tissue type plasminogen activator , and plasminogen activator inhibitor 1 levels were increased in all RCFS patients , suggesting that RCFS , whether MMC induced , or due to TTP or DIC , might be associated with vascular endothelial cell injury . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) and 2 ( PAI 2 ) , tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) and D dimers ( D D ) were quantified in plasma samples and pleural effusion specimens . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of thrombin antithrombin 3 complex ( TAT ) , D dimer , plasmin ( alpha 2 antiplasmin complex ( PAP ) , tissue type plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) 1 antigen were followed in twelve patients treated with direct PTCA for AMI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin time , activated partial thromboplastin time , fibrinogen , antithrombin 3 , tissue type plasminogen activator ( tPA ) activity , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , and platelet aggregation were measured and compared to those in an age matched control group ( C , n = 7 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of plasminogen activator inhibitor 1 , tissue type plasminogen activator , and von Willebrand factor antigens were also significantly increased in patients with PE . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) is the rapid physiologic inhibitor of tissue type plasminogen activator and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It also mediates the cellular uptake of the uncomplexed protease tissue type plasminogen activator ( tPA ) , suggesting that the tPA and PAI 1 binding sites on LRP are in close vicinity and might promote cooperative binding of tPA PAI 1 complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured the antigen concentrations or activities of plasminogen , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) , alpha 2 antiplasmin , alpha 1 antitrypsin , and alpha 2 macroglobulin as well as total antiplasmin activity and D dimer concentration in the plasma of 39 patients with nephrotic syndrome and 32 normal controls subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The main components of the murine plasma fibrinolytic system , including fibrinogen , plasminogen , alpha 2 antiplasmin , tissue type plasminogen activator and plasminogen activator inhibitor 1 , were purified to homogeneity and their interactions were quantitated and compared with those of the human counterparts . ^^^ Murine and human plasmin were very rapidly inhibited by autologous and heterologous alpha 2 antiplasmin ( second order inhibition rate constants , k 1 , app , of 2 . 1 2 . 3 10 10 ( 7 ) M 1 s 1 ) and murine and human tissue type plasminogen activator were very rapidly inhibited by autologous or heterologous plasminogen activator inhibitor 1 ( k 1 , app of 1 . 8 4 . 9 10 10 ( 7 ) M 1 s 1 ) . ^^^ Two chain murine tissue type plasminogen activator ( added at a concentration of 1 microgram / ml ) was inhibited in normal or plasminogen activator inhibitor 1 deficient murine plasma with half lives of 6 . 5 min and 4 . 2 min , respectively , as compared to 80 min for human tissue type plasminogen activator , suggesting that murine plasma contains proteinase inhibitors other than plasminogen activator inhibitor 1 which efficiently inhibit autologous tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients ' plasma levels of ATM ( thrombin induced modified antithrombin 3 ) , FbDP ( fibrin degradation products , D Dimers ) , t PA ( tissue type plasminogen activator ) and PAI 1 ( plasminogen activator inhibitor type 1 ) were measured at regular intervals . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The low density lipoprotein receptor related protein / alpha 2 macroglobulin receptor ( LRP ) mediates endocytosis of a number of structurally unrelated ligands , including complexes of plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) or urokinase plasminogen activator ( u PA ) , free t PA , single chain urokinase ( pro u PA ) , alpha 2 macroglobulin protease complexes , and lipoprotein lipase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern analysis of poly A+RNA prepared from cultured human mesangial cells revealed mRNA for tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Direct and reverse zymography indicated that this reduction resulted from two simultaneous events , a pronounced decrease in tissue type plasminogen activator activity and an induction of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the role of mesothelial cells ( MC ) in the high peritoneal fibrin turnover we investigated the expression of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tissue factor in cultured human peritoneal MC under basal conditions and after exposure to tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , or bacterial lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the relationship between fasting insulin level and various hemostatic factors , including fibrinolytic factors ( active plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) PAI 1 complex , plasmin alpha 2 plasmin inhibitor ( PIC ) , and D dimer ) , coagulation factors ( activated factor 7 , factor 7 coagulant activity and antigen , factor 8 , factor 10 , and fibrinogen ) , coagulation inhibitors ( antithrombin 3 , heparin cofactor 2 , and protein C ) , and an acute phase marker ( sialic acid ) in 102 healthy individuals aged > or = 75 years ( 46 men and 56 women ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Complex formation with target proteases , tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , caused decreased orientational freedom of BDYIA in the P 3 position , while the orientational freedom of BDYIA in position P 1 ' increased to a level similar to that of BDYIA in reactive center cleaved PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a primary endogenous inhibitor of tissue type plasminogen activator ( t PA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a primary endogenous inhibitor of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activity and immunoreactivity of tissue type plasminogen activator ( tPA ) as a protease for angiogenesis and the immunoreactivity of its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , were measured in conditioned medium of HUVEC stimulated for 24 h with saponin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to determine whether the T cell lymphokines interleukin 4 ( IL 4 ) and gamma interferon ( gamma IFN ) affect SMC fibrinolysis and migration , we examined the effects of human recombinant IL 4 and gamma IFN on human aortic SMC tissue type plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen production , as determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator ( t PA ) antigen and activity and plasminogen activator inhibitor 1 ( PAI 1 ) antigen were determined at rest and in response to a venous occlusion test . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma fibrinolytic system was evaluated by determining activities and total plasma concentrations of both tissue type plasminogen activator before and after venous occlusion , and its inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ PAI 1 antigen tended to parallel PAI 1 activity , and levels of tissue type plasminogen activator antigen and activity tended to be lower in the hypertensives at baseline and after venous occlusion , but not significantly different from those in the normotensives . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) in transplanted kidneys ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of aspirin or endotoxin or aspirin plus endotoxin on the circadian variations of plasminogen activator activity ( PAA ) , tissue type plasminogen activator ( t PA ) antigen level , plasminogen activator inhibition ( PAI ) and plasmin inhibition ( PI ) in aorta , heart and brain of the rat was studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma PAI activity was determined by the capacity of plasma to inhibit tissue type plasminogen activator activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study we have delineated in detail the fibrinolytic response to a bolus injection of endotoxin in non human primates and analysed the contribution of cytokines and thrombin generation to the endotoxin induced release of tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^ The administration of pentoxifylline strongly attenuated the release of tissue type plasminogen activator and plasminogen activator inhibitor 1 , whereas the antitumour necrosis factor antibodies blocked the fibrinolytic response entirely . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To elucidate the pathophysiology of idiopathic pulmonary fibrosis ( IPF ) , we examined procoagulant ( tissue factor : TF ) , fibrinolytic ( tissue type plasminogen activator : t PA and urokinase type plasminogen activator : u PA ) and antifibrinolytic ( plasminogen activator inhibitor 1 : PAI 1 and PAI 2 ) activities in bronchoalveolar lavage ( BAL ) supernatant fluids and BAL cell lysates obtained from IPF patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) are produced by osteoblasts , as are the specific inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) and a cellular receptor for uPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Holter ST segment recording and pretreatment plasma tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , prothrombin fragment F 1 + 2 and soluble fibrin levels were measured in 57 patients with acute evolving myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study investigated the effect of carbazochrome sodium sulphonate ( AC 17 ) on the accumulation of tissue type plasminogen activator antigen ( t PA : Ag ) and plasminogen activator inhibitor 1 antigen ( PAI 1 : Ag ) in culture medium of human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The assay is based on polyclonal antibodies , which were found to be slightly more reactive with tissue type plasminogen activator ( t PA ) / PAI 1 complexes and latent PAI 1 than with active PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the most important inhibitor of tissue type plasminogen activator ( t PA ) in plasma and plays a major role in the regulation of fibrinolysis . ^^^ Cathepsin D like aspartyl protease activity mediates the degradation of tissue type plasminogen activator / plasminogen activator inhibitor 1 complexes in human monocytes . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the most important inhibitor of tissue type plasminogen activator ( t PA ) in plasma and plays a major role in the regulation of fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Resting and post venous occlusion euglobulin clot lysis time , tissue type plasminogen activator ( t PA ) activity , t PA antigen , plasminogen activator inhibitor ( PAI ) activity and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen determined on the two occasions did not differ significantly . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We previously demonstrated that cultured human umbilical vein endothelial cells ( HUVECs ) overlaid with a fibrin clot induced a slight increase in tissue type plasminogen activator ( t PA ) secretion and marked reduction in plasminogen activator inhibitor 1 ( PAI 1 ) secretion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HMEC 1 is a SV 40T transfected human microvascular endothelial cell line that constitutively expresses RNA transcripts for plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) , protein S ( PS ) , von Willebrand factor ( vWF ) , and thrombomodulin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Biological effects of disruption of the tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 genes in mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the effects of lipoprotein ( a ) [ Lp ( a ) ] on the fibrinolytic system in patients with acute myocardial infarction ( AMI ) who underwent thrombolytic therapy with recombinant tissue type plasminogen activator , we examined serial changes in plasma levels of Lp ( a ) , plasminogen activator inhibitor ( PAI ) activity , alpha 2 plasmin inhibitor plasmin complex ( PIC ) and thrombin antithrombin 3 complex ( TAT ) in venous plasma samples from 25 patients with AMI for 3 weeks . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated immunohistochemically the localization of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasmin inhibitor ( PI ) , and transforming growth factor beta ( TGA beta ) in tissue sections to examine the relationships between their localization and local invasiveness , tumor size and cervical lymph node metastasis of head and neck squamous cell carcinomas ( SCCs ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of the plasminogen activators ( PAs ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^ Identification of tissue type plasminogen activator specific plasminogen activator inhibitor 1 mutants . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of the plasminogen activators ( PAs ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI ) activity , and von Willebrand factor antigen ( vWF : Ag ) and activity ( vWF : RCof ) were measured in 21 SLE patients ( 12 of whom were therapy free ) and 22 controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There have been reports of abnormalities in the antigenic levels or activity of endothelium derived haemostatic factors , such as tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) ; however the data from these studies are conflicting . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both the decrease in tissue type plasminogen activator ( t PA ) and the increase in plasminogen activator inhibitor 1 ( PAI 1 ) caused by ischemia reperfusion were also significantly suppressed by EGb 761 treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The role of the finger domain of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in initiation and progression of thrombolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma thrombin antithrombin 3 complex ( TAT ) , FDP D dimer , activated protein C ( APC ) protein C inhibitor ( PCI ) complex , and tissue type plasminogen activator ( t PA ) , PA inhibitor 1 ( PAI 1 ) were significantly increased in patients with acute myocardial infarction ( AMI ) at onset . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We determined serially in the patients up to 10 days after admission factor 12 and the beta factor XIIa inhibition , kallikrein like activity , prekallikrein , C 1 esterase inhibitor , kallikrein inhibition , high molecular weight kininogen as indicators of the contact phase and bradykinin generation , thrombin antithrombin 3 ( TAT ) complex as marker of the activated coagulation cascade , fibrinogen , plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( TPA ) , and D dimers as indicators of the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The second order association rate of PAI 1 R346M with tissue type plasminogen activator is over 10 , 000 fold lower than that of wild type PAI 1 , whereas the ability of the variant to bind to fibrin is unaltered . ^^^ Fibrinolysis , mediated by tissue type plasminogen activator , is inhibited in a dose dependent manner by purified PAI 1 . ^^^ The significance of fibrin binding by plasminogen activator inhibitor 1 for the mechanism of tissue type plasminogen activator mediated fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect was specific for PAI 1 because tissue type plasminogen activator ( t PA ) and expression of procoagulant activity were not affected by either lipoprotein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this investigation we show that uPAR levels correlate with uPA levels in human breast cancers . uPAR levels , however , do not correlate with other components of the plasminogen activator system such as tissue type plasminogen activator ( t PA ) , PAI 1 or PAI 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , we showed that human umbilical vein endothelial cells grown on polytetrafluoroethylene or polyurethane released more plasminogen activator inhibitor 1 and tissue type plasminogen activator into the conditioned media than did human umbilical vein endothelial cells grown on tissue culture polystyrene . ^^^ Our results give evidence that human umbilical vein endothelial cells grown on two biomaterials used to construct vascular grafts , namely polytetrafluoroethylene and polyurethane , produce tissue type plasminogen activator as well as plasminogen activator inhibitor 1 , two major components of the fibrinolytic system also expressed by endothelial cells in vivo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study the expression of tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) antigen in conditioned medium from cultured human umbilical vein ( HUVEC ) and human saphenous vein ( HSVEC ) endothelial cells was investigated under basal conditions and after stimulation with LPS , TNF alpha , interferon gamma ( IFN gamma ) or interleukin 6 ( IL 6 ) alone or in combinations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To clarify the changes which occur postoperatively in intravascular fibrinolysis , plasma levels of tissue type plasminogen activator ( t PA ) antigen , the total plasminogen activator inhibitor type 1 ( PAI 1 ) antigen , and the t PA PAI 1 complexes were assayed in this study . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : In 107 consecutive patients undergoing coronary angioplasty , we measured plasma levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , von Willebrand factor , and fibrinogen before and immediately after angioplasty and at a 6 month follow up . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We studied the association of plasminogen activator inhibitor antigen ( PAI 1 ) , tissue type plasminogen activator antigen ( TPA ) , and D dimer with early atherosclerosis in a cross sectional case control study involving 457 pairs chosen from the biracial cohort of the Atherosclerosis Risk in Communities ( ARIC ) Study . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of heparin on the mesangial plasminogen activating system ( tissue type plasminogen activator , t PA , and plasminogen activator inhibitor type 1 , PAI 1 ) , which is thought to be involved in matrix remodelling , has not been previously reported . ^^^ Heparin selectively inhibits synthesis of tissue type plasminogen activator and matrix deposition of plasminogen activator inhibitor 1 by human mesangial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This TM spike was not correlated with the progressive tissue type plasminogen activator ( t PA ) increase , plasminogen activator inhibitor 1 ( PAI 1 ) , or von Willebrand factor ( vWF ) values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In combination with a single bolus injection of recombinant tissue type plasminogen activator ( rTPA ; 0 . 25 mg / kg ) , the anti PAI 1 antibody reduced thrombus growth significantly , from 21 . 5 + / 2 . 7 % in the animals treated with rTPA alone to 12 . 2 + / 2 . 6 % in the animals treated with rTPA and the antibody . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of the vascular endothelial cell markers , thrombomodulin ( TM ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) , and von Willebrand factor ( vWF ) were measured in 55 patients on maintenance haemodialysis ( HD ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The factors related to the initiation of fibrinolysis , especially with regard to the tissue type plasminogen activator ( tPA ) and the plasminogen activator inhibitor 1 ( PAI 1 ) , were investigated in 15 patients who underwent hepatic resection , and the findings were compared between those with normal livers and those with diseased livers . ^^^ Initiation of a fibrinolytic system in hepatic resection : the roles of tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor C 1 ( PAI 1 ) plays a critical role in the regulation of fibrinolysis , serving as the primary inhibitor of tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were identified as the primary plasminogen activator and plasminogen activator inhibitor , respectively , in equine blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels were determined in maternal and cord plasma of tissue type plasminogen activator antigen and activity , plasminogen activator inhibitor 1 antigen , plasminogen activator inhibitor activity , and plasminogen activator inhibitor 2 antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Neither drug had an effect on tissue type plasminogen activator or plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ The long term effects of metoprolol and epanolol on tissue type plasminogen activator and plasminogen activator inhibitor 1 in patients with ischaemic heart disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements were made of serum estradiol , and the plasma concentrations of tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI 1 ) antigen , antithrombin 3 ( AT 3 ) and protein C activity , factor 7 ( FVII ) antigen , and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured fibrinogen levels as well as the fibrinolytic parameters tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in plasma samples obtained at basal conditions and after stimulating the fibrinolytic system by venous occlusion ( VO ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether previously reported abnormalities in fibrinolytic activity and plasma lipoprotein ( a ) levels could reflect obesity rather than diabetes per se , plasma concentrations of tissue type plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , and lipoprotein ( a ) ( Lp ( a ) ) were investigated in sixty four type 2 diabetic patients ( 56 . 1 + / 9 . 5 years ; body mass index , 24 . 6 + / 3 . 3 kg / m2 ) and thirty two control subjects ( 57 . 9 + / 8 . 9 years ; body mass index , 24 . 6 + / 3 . 4 kg / m2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A positive family history for ischemic complications of atherosclerosis was more common in subjects with a history of stroke than in those without ; moreover , plasma levels of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( TPA ) were higher in patients with documented previous events . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TGF alpha enhanced the expression of the tissue type plasminogen activator ( t PA ) gene , whereas TGF beta increased the expression of the PA inhibitor 1 ( PAI 1 ) gene and inhibited that of the t PA gene . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Samples were assayed for coagulation factors ( fibrinopeptide A , antithrombin 3 , protein C ) , fibrinolytic factors [ tissue type plasminogen activator ( t PA ) , alpha 2 antiplasmin , plasminogen activator inhibitor ( PAI 1 ) ] and markers of platelet activation ( platelet factor 4 , beta thromboglobulin ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Variables determined were tissue type plasminogen activator ( t PA ) and the plasminogen activator inhibitors type 1 ( PAI 1 ) and type 2 ( PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Comparative thrombolytic properties of tissue type plasminogen activator and of a plasminogen activator inhibitor 1 resistant glycosylation variant , in a combined arterial and venous thrombosis model in the dog . rt PA K , a variant of recombinant tissue type plasminogen activator ( rt PA ) with substitution of amino acids 296 to 299 with alanine ( KHRR 296 299AAAA ) has increased fibrin specificity and reduced sensitivity to plasminogen activator inhibitor 1 ; rt PA T , with threonine 103 replaced by asparagine has an additional glycosylation site and a reduced clearance ; and rt PA N , with asparagine 117 mutagenized to glutamine lacks the high mannose carbohydrate side chain . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma D dimers ( one ELISA and two latex assays ) , fibrin monomer , prothrombin fragment 1 + 2 ( F1+2 ) , thrombin antithrombin 3 complex ( TAT ) and the t PA PAI 1 complex were all significantly correlated to the extension of DVT , whilst fibronectin , tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( scru PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were not . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When mRNA obtained from RAECs cultured for 16 hours in serum free medium was analysed for the presence of specific messages of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) using human probes , weak specific binding could be seen for both t PA and mu PA while the PAI 1 probe gave a strong specific signal at 3 . 4 Kb and a weak signal at 2 . 3 Kb . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It is demonstrated that 1 ) theca interstitial compartment synthesizes the majority of plasminogen activator inhibitor type 1 ( PAI 1 ) in the ovary before ovulation , and the follicular wall may therefore serve as a specific barrier with the presence of PAI 1 activity to prevent the secretion of tPA into the extrafollicular compartments ; 2 ) granulosa cells secrete only a small amount of ovarian PAI 1 , but synthesize the most of tissue type plasminogen activator tPA involved in the processes leading to ovulation ; 3 ) since only matured cumulus oocyte complexes secrete a large amount of tPA and PAI 1 , both tPA and PAI 1 activity in the conditioned medium may be used as reliable markers for evaluating oocyte quality for in vitro fertilization . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant differences were observed between the control and IUGR groups in relation to the other fibrinolytic parameters , except for plasma PAI type 1 and tissue type plasminogen activator levels , which were significantly increased in the PE group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate fibrinolysis at the level of plasminogen activation under conditions of a dynamic test , using methods which reflect sensitively and specifically the activity of the tissue type plasminogen activator ( t PA ) and the activity of the inhibitor of the tissue type plasminogen activator ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of notoginsenoside R 1 on the synthesis of tissue type plasminogen activator and plasminogen activator inhibitor 1 in cultured human umbilical vein endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The expression of PAI 1 and tissue type plasminogen activator ( TPA ) mRNA in cultured rat VSM cells was studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human colorectal carcinogenesis has been shown previously to be associated with impressive changes in the tissue levels of plasminogen activators and their inhibitors , exemplified by an increase in the urokinase type plasminogen activator ( u PA ) and the inhibitors PAI 1 and PAI 2 , and a decrease in tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the main physiological inhibitor of tissue type plasminogen activator ( t PA ) , may occur in three interconvertible conformations : active , latent , and substrate . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the main physiological inhibitor of tissue type plasminogen activator ( t PA ) , may occur in three interconvertible conformations : active , latent , and substrate . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : To determine if the failure to lyse pulmonary thromboemboli is caused by an abnormality in the endothelial cell ( EC ) associated fibrinolytic system , conditions were established to culture ECs from patient main pulmonary arteries during surgical pulmonary thromboendarterectomies and to analyze the conditioned media for levels of tissue type plasminogen activator ( TPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We assayed urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) in 43 human brain tumors ( predominantly astrocytic gliomas ) and in histologically disease free brain tissue resected with 21 of the tumors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study was to investigate , in this high risk population , whether unstable angina was associated with abnormalities of tissue type plasminogen activator ( t PA ) or plasminogen activator inhibitor ( PAI ) activities and whether , in a prospective study , any of these parameters would identify patients with an adverse cardiovascular prognosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator antigen ( t PA : Ag ) , plasminogen activator inhibitor 1 antigen ( PAI 1 : Ag ) , the active form of PAI 1 ( active PAI ) and t PA . ^^^ Fluctuations of tissue type plasminogen activator . plasminogen activator inhibitor 1 complex in patients with DIC ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the primary physiological inhibitor of tissue type plasminogen activator , is an unusual member of the serine protease inhibitor ( serpin ) superfamily in that it spontaneously converts to a latent form lacking activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In HUVEC , the ratio of fibrinolysis antigens was antifibrinolytic , consisting of 55 fold more plasminogen activator inhibitor type 1 ( PAI 1 ) than tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The role of plasminogen activator inhibitor type 1 ( PAI 1 ) in the clearance of tissue type plasminogen activator ( t PA ) by hepatocyte like cells was studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We compared the plasma TM levels with the plasma levels of other specific markers of endothelial cell activation such as : prostacyclin ( PGI 2 ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effects of Guan Mai Shu ( GMS ) , a traditional Chinese medicine preparation , on tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) in the plasma of patients with coronary heart disease were studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TRAP induced rapid morphological changes in HUVECs , with marked increase in the release of prostacyclin , endothelin , platelet activating factor , tissue type plasminogen activator , and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of isotretinoin on endogenous tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in humans . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelets contain a great deal of plasminogen activator inhibitor 1 ( PAI 1 ) , the principal endogenous inhibitor of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
With regard to the initial stage of the fibrinolytic system , such as tissue type plasminogen activator and plasminogen activator inhibitor 1 , there was no difference between the groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Regulation of endothelial cell ( EC ) plasminogen activator inhibitor type 1 ( PAI 1 ) , the primary physiological inhibitor of tissue type plasminogen activator ( TPA ) and urokinase type plasminogen activator ( UPA ) , by various stimuli has been well characterized . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) ] . ^^^ Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) are the most important factors involved in the regulation of blood fibrinolysis . t PA converts zymogen plasminogen to plasmin on the surface of endothelial cells to maintain blood fluidity and on the surface of the thrombus to efficiently lyse the thrombus . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It is not clear whether loss of endogenous ovarian steroid production or the effects of the orally administered contraceptive steroids cause significant changes in hemostatic factors ( antithrombin 3 , protein S , protein C , plasminogen , tissue type plasminogen activator , plasminogen activator inhibitor 1 , histidine rich glycoprotein , and 7 , 8 , 10 , 12 coagulation factors ) during OC use . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , a member of the serpin family of serine protease inhibitors , inhibits both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Zymographic analyses showed that tissue type plasminogen activator ( t PA ) occurred in association with casein micelles , partially as a complex with type 1 plasminogen activator inhibitor ( PAI 1 ) , whereas urokinase type plasminogen activator ( u PA ) was confined to milk leukocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Parameters of fibrinolysis , including basal plasma tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen levels were studied in 49 non insulin dependent diabetic patients ( 23 men , 26 women : ages 51 . 3 + / 14 . 9 years ) and 16 age matched non diabetic subjects ( 9 men , 7 women ages 49 . 8 + / 12 . 2 years ) as a control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : To determine if the failure to lyse pulmonary thromboemboli is caused by the local expression of the primary inhibitor of tissue type plasminogen activator ( type 1 plasminogen activator inhibitor , PAI 1 ) , levels of PAI 1 antigen and mRNA were analyzed by immunohistochemistry and in situ hybridization in specimens harvested from a series of patients during pulmonary thromboendarterectomies . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitors in the blood , that is , notably plasminogen activator inhibitor type 1 ( PAI 1 ) , bind circulating tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Reduced GFA in fibrin associated GN was associated with decreased expression of tissue type plasminogen activator ( tPA ) and increased expression of plasminogen activator inhibitor type 1 ( PAI 1 ) and glomerular macrophage infiltration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These haemodynamic responses are associated with increments in the plasma levels of renin , noradrenaline ( NA ) , clotting factor 8 ( FVIII : C ) , von Willebrand factor ( vWF : ag ) , and tissue type plasminogen activator ( t PA ) , and a fall in the plasma level of plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic capacity of blood depends mainly on the amount of tissue type plasminogen activator ( t PA ) activity and plasminogen activator inhibitor type 1 ( PAI 1 ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In our study , we measured resting tissue type plasminogen activator ( t PA ) activity and resting plasminogen activator inhibitor 1 ( PAI 1 ) activity in 15 healthy men and in 20 patients ( male ) with coronary heart disease in the morning and evening . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of plasma tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , 8 factor related antigen ( VIIIR : Ag ) and anti thrombin ( AT 3 ) were determined in 40 hypertensive patients with Blood Stasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
SDS gel electrophoresis of plasma incubated with 125I labeled recombinant human tissue type plasminogen activator revealed an approximately 115 , 000 M ( r ) component with plasma from endotoxin stimulated ( 0 . 5 mg / kg ) PAI 1+ / + but not from PAI 1 / mice , which could be precipitated with a polyclonal anti PAI 1 antiserum . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
SMC of different origin responded to thrombin treatment with a dose and time dependent increase in tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) levels in both cell lysates and conditioned media with maximum effects achieved at 10 20 IU / ml thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to elucidate if this decreased response is due to an increase in plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) , and / or to decreased release of tissue type plasminogen activator ( t PA ) antigen during venous occlusion , 36 women ( 18 women with normal pregnancy and 18 with gestational hypertension without proteinuria ) were followed during pregnancy and puerperium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Both tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) are synthesized by vascular endothelium , whereas hepatocytes synthesize PAI 1 but not t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue type plasminogen activator ( t PA ) level was increased , but it was well correlated with the plasminogen activator inhibitor 1 ( PAI 1 ) level . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To assess the actual degree of activation of coagulation systems and vascular disorders in hypercholesterolemia , plasma levels of vascular endothelial cell markers , such as thrombomodulin ( TM ) , tissue type plasminogen activator , plasminogen activator inhibitor 1 ( PAI 1 ) , and von Willebrand factor , were measured in 51 patients with hypercholesterolemia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The recovery of the different molecular forms of PAI 1 , relative to active PAI 1 ( 100 % ) , was 99 % + / 7 % for PAI 1 complexed with recombinant tissue type plasminogen activator ( t PA ) , 104 % + / 4 % for PAI 1 complexed with melanoma t PA , 94 % + / 11 % for PAI 1 complexed with high M ( r ) urokinase , and 113 % + / 3 % for latent PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was performed to determine whether elaboration of PAI 1 from platelets activated physiologically by thrombolysis of pre formed clots inhibits activation of plasminogen by tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recent findings have elucidated the mechanism for clearance from the extracellular space of the two types of plasminogen activators , urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) , and their type 1 inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Quantitative zymography and enzyme linked immunosorbent assay were used to determine the amounts of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator , and plasminogen activator inhibitors type 1 and type 2 ( PAI 1 and PAI 2 ) in benign and malignant primary brain tumors ( n = 28 ) as well as nonneoplastic brain ( n = 5 ) . u PA and PAI 1 antigen were undetectable in normal brain but significantly elevated in glioblastoma multiforme ( u PA , 2 . 86 + / 3 . 01 ng / mg ; PAI 1 , 8 . 19 + / 5 . 57 ng / mg ; P < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 mRNA expression correlated with glomerular PAI 1 bioactivity as determined by a functional tissue type plasminogen activator ( t PA ) binding assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether oxidized low density lipoprotein ( Ox LDL ) , which accumulates in atherosclerotic arteries , modulates the endothelial fibrinolytic system , cultures of human umbilical vein endothelial cells were incubated with low density lipoproteins or lipids , and levels of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) antigens in the conditioned medium were measured by enzyme linked immunosorbent assay . ^^^ Transferable lipids in oxidized low density lipoprotein stimulate plasminogen activator inhibitor 1 and inhibit tissue type plasminogen activator release from endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , and plasminogen activator inhibitor 2 were also measured by means of enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In our search for compounds that can stimulate endogenous fibrinolysis , we have found that certain triazolobenzodiazepines enhance the production of tissue type plasminogen activator ( t PA ) by vascular endothelial cells maintained in vitro , with no or even a lowering effect on plasminogen activator inhibitor type 1 ( PAI 1 ) production . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Extrapolation of these in vitro results to periimplantational events in humans suggests that under progesterone regulation , decidual cell derived PAI 1 could 1 ) restrain blastocyst invasion of the stroma by inhibiting trophoblast associated urokinase type plasminogen activator , and 2 ) prevent hemorrhage during trophoblast invasion of the endometrial vasculature by inhibiting fibrinolysis mediated by tissue type plasminogen activator . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of LDL and acetyl LDL was concentration dependent and specific for PAI 1 because tissue type plasminogen activator and expression of procoagulant activity were not affected by either lipoprotein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have explored TMp in patients with atheromatous disease and compared its level with others endothelial cell markers , particularly those who indicate cell activation , as tissue type plasminogen activator ( t PA ) , inhibitor of plasminogen activator ( PAI 1 ) and prostacyclin ( PG 12 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured the plasma concentrations of tissue type plasminogen activator ( t PA ) , PAI 1 and t PA PAI complex before and serially after thrombolytic therapy for acute myocardial infarction to clarify the relationship between thrombolytic therapy and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The variables measured were serum oestradiol content , plasma antithrombin 3 ( AT 3 ) activity , protein C activity and the plasma concentrations of tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI 1 ) antigen , and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Moreover , purified TGF beta 1 produces a Schwann cell morphology alteration and decreases the secretion of tissue type plasminogen activator ( tPA ) and enhances the secretion of plasminogen activator inhibitor ( PAI ) by Schwann cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements of plasminogen activator inhibitor 1 ( PAI 1 ) antigen , tissue type plasminogen activator ( t PA ) antigen , D dimer antigen , factor 7 activity , fibrinogen , and protein C antigens were made at baseline and after 6 months in 90 men and 88 women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured PAI 1 activity and antigen and tissue type plasminogen activator ( t PA ) antigen in 35 ( 20 nondiabetic and 15 diabetic ) subjects with no clinical or electrocardiographic evidence of ischemic heart disease and in 74 ( 50 nondiabetic and 24 diabetic subjects ) who had survived a myocardial infarction in the preceding 6 24 months . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the physiologic inhibitor of both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , is a major biosynthetic product of endothelial cells in vitro ; endothelial cells in vivo , in contrast , do not appear to produce significant amounts of PAI 1 as made evident by in situ hybridization studies in normal mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have investigated the role of plasminogen activator inhibitor 1 ( PAI 1 ) in the regulation of fibrinolysis using a model thrombus composed of thrombin stimulated platelets , fibrin ( ogen ) , plasminogen , and recombinant tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were obtained on days 1 , 4 , and 7 after hospital admission to measure tissue type plasminogen activator antigen ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor antigen ( PAI 1 ) , plasminogen , alpha 2 antiplasmin , fibrinogen , antithrombin 3 , protein C , protein S , thrombin antithrombin complexes ( TAT ) , D dimer , and von Willebrand factor related antigen ( vWF : Ag ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study the effect of vitamin A status and retinoid treatment on the activity of tissue type plasminogen activator ( TPA ) and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , in plasma and in several tissues was investigated in BN / BiRij rats . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In four normotensive subjects and six hypertensive patients , we investigated whether infusion of angiotensin 2 ( Ang 2 ) affected circulating levels of plasminogen activator inhibitor 1 ( PAI 1 ) , the most important physiological inhibitor of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) levels produced by HFMEC remained unaffected by IL 4 , whereas tissue type plasminogen activator ( t PA ) levels were slightly decreased when HFMEC were treated with IL 4 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using gonadotropin induced ovulation as a model , we have studied how two components of the PA system , tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , are regulated temporally and spatially by gonadotropins , leading to the initiation and termination of a well directed proteolytic process . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We , therefore , performed immunohistological studies on human colon tumours using monoclonal antibodies against urokinase ( u PA ) and tissue type plasminogen activator ( t PA ) as well as against plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This cell line synthesized prostacyclin , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) as well as produced the proform of matrix metalloproteinase 1 , which was activated by cultivating the cells with plasminogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study compared the activities of recombinant tissue type plasminogen activator ( t PA ) and a plasminogen activator inhibitor 1 ( PAI 1 ) resistant variant t PA ( Kt PA , KHRR 296 299 AAAA ) in preventing renal fibrin deposition in rabbits with elevated PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Mice with combined homozygous deficiency of tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) ( T U ) , of t PA and plasminogen activator inhibitor 1 ( PAI 1 ) ( T P ) , of u PA and PAI 1 ( U P ) or of t PA , u PA , and PAI 1 ( T U P ) were generated by inbreeding of mice with the respective deficiencies . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activity and antigen , euglobulin fibrinolytic activity , tissue type plasminogen activator activity and antigen urokinase type plasminogen activator antigen , plasminogen activator inhibitor 1 activity and antigen , plasminogen activator inhibitor 2 antigen , tissue type plasminogen activator / plasminogen activator inhibitor complexes , alpha 2 antiplasmin , histidine rich glycoprotein , and fibrinogen / fibrin degradation products were measured in blood samples taken from the umbilical vein of 100 healthy full term newborns . ^^^ There was a significant reduction in plasminogen activity and antigen ( P < 0 . 0001 ) , plasminogen activator inhibitor 1 activity ( P < 0 . 05 ) , alpha 2 antiplasmin ( P < 0 . 0001 ) , and histidine rich glycoprotein ( P < 0 . 0001 ) , whereas plasminogen activator inhibitor 2 , tissue type plasminogen activator / plasminogen activator inhibitor complexes and fibrinogen / fibrin degradation products did not differ between groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , fibrinogen ( Fg ) , thromboxane B 2 ( TXB 2 ) , 6 ketoprostaglandin F 1 alpha ( 6 keto PGF 1 alpha ) , renin activity ( PRA ) and angiotensin 2 ( ANGII ) were assayed in 18 patients with coronary heart disease and 11 healthy subjects before and after submaximal treadmill exercise test according to `` Bruce programme ' ' . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Then , in order to investigate the mechanisms of hyperfibrinolysis in liver cirrhosis , plasma levels of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were determined in these patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Additionally , the enzyme is not inhibited by plasminogen activator inhibitor 1 which is the primary physiological inhibitor of both urokinase and tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After assay of accumulated release of tissue type plasminogen activator ( t PA ) and endothelin 1 ( ET ) , cells were stimulated with 1 U / ml of human thrombin for 1 h and medium collected for RIA of 6 keto prostaglandin F 1 alpha ( 6 keto PGF 1 alpha ) , thrombospondin ( TSP ) , von Willebrand factor ( vWf ) and ELISA of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Within 5 days , smooth muscle cells ( SMCs ) on the luminal surface expressed the mRNA for tissue type plasminogen activator ( TPA ) , urokinase type plasminogen ( UPA ) , the receptor for UPA ( UPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was undertaken in order to determine the recognition site for tissue type plasminogen activator plasminogen activator inhibitor type 1 [ t PA PAI 1 ] complexes in rat liver in vivo and in vitro . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Three enzyme linked immunosorbent assays for the quantitation of murine tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , were developed using monoclonal antibodies raised against the autologous proteins in gene inactivated mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HB EGF enhanced the mRNA expression of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) mRNA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effect of exogenous staphylococcal sphingomyelinase ( SMase ) on the release of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) from cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using an adult pseudopregnant rat model , we have studied how two components of the PA system , tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , are expressed temporally and spatially during different developmental stages of the corpus luteum ( CL ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , single deficient mice lacking tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , or PA inhibitor type 1 ( PAI 1 ) gene function were recently found to have normal reproduction , although mice with a combined deficiency of tPA and uPA were significantly less fertile . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator ( TPA ) and plasminogen activator inhibitor 1 help identify subjects with a history of cerebral ischemic episodes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a member of the serpin superfamily of proteins and is the fast acting inhibitor of both urinary plasminogen activator and tissue type plasminogen activator . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a member of the serpin superfamily of proteins and is the fast acting inhibitor of both urinary plasminogen activator and tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentrations of tissue type plasminogen activator antigen and activity as well as plasminogen activator inhibitor 1 antigen and activity were measured under resting conditions in 12 hypertensive ( 69 . 4 + / 1 . 4 years ) and 11 normotensive 65 . 2 + / 1 . 3 years ) older men . ^^^ In addition , the molar concentration ratio of active tissue type plasminogen activator to active plasminogen activator inhibitor 1 did not differ between the hypertensive ( 1 : 9 . 7 + / 2 . 3 ) mmol / L ) and normotensive ( 1 : 10 . 5 + / 2 . 2 mmol / L ) subjects , indicative of no impairment in fibrinolytic potential in either group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and interleukin 1beta were all found to be significantly different in interfaces and in pseudocapsules , compared to controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of PAI 1 activity and tissue type plasminogen activator ( TPA ) activity or antigen were unaffected by gemfibrozil treatment both at rest and during the provocations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On diagnosis , blood was drawn , and plasma levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) , D dimer ( DD ) , and tissue factor pathway inhibitor ( TFPI ) were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of chronic stress on tissue type plasminogen activator ( TPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigens was studied in 69 healthy middle aged men . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
They also had significantly ( P < . 05 ) higher plasma fibrinogen , factor 8 clotting activity , tissue type plasminogen activator antigen , and plasminogen activator inhibitor 1 ( PAI 1 ) activity ( 19 . 2+ / 2 . 4 versus 8 . 5+ / 1 . 6 AU / mL , P < . 001 ) and lower basal tissue type plasminogen activator activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study assessed the short and long term effects of an energy restrictive diet with or without exercise on plasminogen activator inhibitor 1 antigen ( PAI 1 Ag ) and PAI 1 activity , tissue type plasminogen activator antigen ( TPA Ag ) , and fibrinogen serum levels . ^^^ Plasminogen activator inhibitor 1 , tissue type plasminogen activator , and fibrinogen : Effect of dieting with or without exercise in overweight postmenopausal women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Phage display technology has been exploited to study in detail the interaction between plasminogen activator inhibitor 1 ( PAI 1 ) and either thrombin or an essential positively charged `` loop ' ' of tissue type plasminogen activator ( t PA ) , denoted variable region 1 ( VR 1 ) . ^^^ Selective screening of a large phage display library of plasminogen activator inhibitor 1 mutants to localize interaction sites with either thrombin or the variable region 1 of tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Univariate analysis showed that a low tissue type plasminogen activator ( t PA ) activity in normal mucosa and in carcinomas and a high antigen level of inhibitor type 1 ( PAI 1 ) , and , to a lesser extent , of urokinase type plasminogen activator ( u PA ) receptor , in carcinomas are associated with a poor overall survival of the patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression of tissue type plasminogen activator ( t PA ) , urokinase type PA ( u PA ) and PA inhibitor 1 ( PAI 1 ) in such treated cells was investigated by specific ELISAs on the protein level and by Northern blotting on the mRNA level . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of both uPA and tissue type plasminogen activator ( tPA ) is the major physiologic regulator of plasminogen activator activity . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of both uPA and tissue type plasminogen activator ( tPA ) is the major physiologic regulator of plasminogen activator activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We investigated the relation of current use of replacement hormones to three measures of plasma fibrinolytic activity : tissue type plasminogen activator ( TPA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , and D dimer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To further investigate possible involvement of the PA system , we quantified immunoreactive urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , both plasminogen activator inhibitors ( PAI 1 and PAI 2 ) and u PA receptor ( u PAR ) in synovial tissue extracts of 14 patients with rheumatoid arthritis ( RA ) and 12 with osteoarthritis ( OA ) . u PA , PAI 1 , PAI 2 and u PAR concentrations were significantly higher in RA than in OA patients . t PA antigen levels were significantly lower in RA than in OA synovial tissue extracts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After extraction plasminogen activator activity ( PAA ) was determined by using a chromogenic substrate assay in the presence or absence of inhibitory antibodies against tissue type plasminogen activator ( t PA ) , urokinase or plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since the possible variation of tissue type plasminogen activator ( TPA ) antigen and plasminogen activator inhibitor 1 ( PAI 1 ) antigen concentrations over time after stroke has been rarely studied , it was examined in plasma from stroke patients in the acute and convalescent phases of the disease and in a control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In patients undergoing gastric surgery for malignant ( n = 18 ) or benign ( n = 21 ) disorders . , blood drawn from the portal vein and a peripheral vein was analysed for tissue type plasminogen activator antigen and activity ( tPA : Ag , tPA : Act ) , single chain urokinase type plasminogen activator activity ( scuPA : Act ) , and plasminogen activator inhibitor antigen and activity ( PAI : Ag , PAI : Act ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Initially elevated PAI 1 concentrations decreased by 50 % , with a simultaneous decrease in the concentration of tissue type plasminogen activator ( t PA ) / PAI 1 complexes but no significant change in t PA suggested a decrease in inhibition of the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of tissue type plasminogen activator ( T PA ) , its inhibitor ( PAI 1 ) and fibrinogen in the blood of patients with type 1 and 2 diabetes mellitus ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effects of IL 1 on the production of tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) and PAI 1 by glomerular cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The hypothesis that tea drinking may protect against coronary heart disease ( CHD ) through effects on clotting as measured by plasma fibrinogen , tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) was tested in 65 healthy volunteers ( 31 men and 34 women ; aged 20 74 years ) in a randomized , blind , placebo controlled , crossover study lasting 10 weeks ( run in phase 2 weeks , tea and placebo phases 4 weeks ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Young and senescent cells were compared in quiescent and activated growth conditions for the secretion of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Production of tissue type plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) in mildly cirrhotic rat liver . ^^^ We have studied the production of tissue type plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) in liver of normal rats and in rats with mild cirrhosis induced by carbon tetrachloride inhalation , to demonstrate the production of these fibrinolytic components and their pathophysiologic role in the liver in vivo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When compared with the control groups circulating tissue type plasminogen activator antigen , and especially plasminogen activator inhibitor type 1 , PAI 1 antigen and activity were significantly increased , the mean increase ranging between 54 % and 140 % in the bypass group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasmin can specifically inhibit the biosynthesis of tissue type plasminogen activator ( t PA ) , but not plasminogen activator inhibitor , type 1 ( PAI 1 ) in endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was performed to investigate the relationship between the concentrations of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) and the CT images in 23 cases of chronic subdural hematomas ( SDHs ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cultured human mesangial cells ( HMC ) derived from normal kidneys have been shown to synthesize tissue type plasminogen activator ( t PA ) and excess amounts of PA inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : When human cerebral microvascular endothelial cells ( HCMEC ) , pial arterial endothelial cells , and middle meningeal arterial endothelial cells were exposed to 10 to 1000 ng / mL recombinant tissue type plasminogen activator ( RTPA ) , urokinase type plasminogen activator ( UPA ) , or streptokinase / plasminogen ( 37 U streptokinase plus 2 mumol / L plasminogen ) for 24 hours , they exhibited concentration dependent decreases in elaboration of PAI 1 of 65 + / 3 % , 48 + / 3 % , and 59 + / 8 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immediately after operation , the ratio of thrombin antithrombin 3 complex to plasmin alpha 2 plasmin inhibitor complex was significantly greater and the ratio of tissue type plasminogen activator to plasminogen activator inhibitor 1 was significantly lower in patients who underwent hepatic resection than comparable ratios in patients who underwent colorectal resection . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Malignant tissue extracts had significantly higher levels of urokinase type plasminogen activator and plasminogen activator inhibitor 1 , but lower levels of tissue type plasminogen activator than normal mucosa . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We compared intraindividual and interindividual variability in the plasma levels of fibrinogen , tissue type plasminogen activator ( TPA ) antigen , plasminogen activator inhibitor ( PAI ) activity , and C reactive protein ( CRP ) in 20 healthy , young individuals and 26 patients with stable angina pectoris ( AP ) who were at higher risk for cardiovascular disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is an important regulator of plasminogen activation , which inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is an important regulator of plasminogen activation , which inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : To elucidate the effect of niceritrol on fibrinolysis and Lp ( a ) levels , we examined plasminogen activator inhibitor ( PAI ) activity , tissue type plasminogen activator ( t PA ) antigen , and serum Lp ( a ) levels before and after administration of niceritrol to coronary artery disease patients with high baseline Lp ( a ) levels ( > or = 20 mg / dl ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Though the secreted antigens for tissue type plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) in the conditioned medium ( CM ) of HUVECs were simultaneously increased at 43 degrees C during this period , the increase in the levels of t PA ( about 26 . 6 fold at 120 min ) was greater than that of PAI 1 ( 1 . 8 fold at 120 min ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of factor 7 clotting activity ( F VIIc ) , plasminogen activator inhibitor 1 ( PAI 1 ) activity and antigen , tissue type plasminogen activator ( t PA ) activity significantly differed in men with liver steatosis ( n = 31 ) as compared with those without steatosis ( n = 33 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since PAI 1 inhibits the 2 major plasminogen activators , tissue type plasminogen activator ( t PA ) and urinary type plasminogen activator ( u PA ) in an equimolar manner it was important to establish the potency of the PAI 1 inhibitor in terms of both t PA and u PA neutralisation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The increase in PAI activity and antigen in septic shock was accompanied by an increase in tissue type plasminogen activator antigen and total fibrin ( ogen ) degradation products and a decrease in alpha ( 2 ) antiplasmin activity ( p < 0 . 006 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : prothrombin fragment 1 + 2 ( F 1 + 2 ) , thrombin antithrombin 3 ( TAT ) , D dimers ( D D ) , factor 7 activated , plasminogen activator inhibitor ( PAI ) , tissue type plasminogen activator ( t PA ) , plasma albumin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
One week before and 1 week after the conditioning programs data were collected for body weight , percentage body fat , VO 2 max and 12 h fasting blood levels of total tissue type plasminogen activator ( t PA ) , tissue type plasminogen activator activity ( t PAa ) , total plasminogen activator inhibitor ( PAI 1 ) , plasminogen activator inhibitor activity ( PAI 1a ) , cholesterol ( CHOL ) , triglycerides ( TG ) , and high density lipoprotein cholesterol ( HDL C ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These results indicate that active PAI 1 accumulates in porcine coronary artery thrombi in concentrations markedly higher than those present in plasma and that PAI 1 may be an important determinant of the known resistance of platelet rich thrombi to lysis by tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin zymographic studies indicated that jararhagin increased tissue type plasminogen activator ( tPA ) activity by the dissociation of a complex of tPA with type 1 plasminogen activator inhibitor ( PAI 1 ) . alpha 2 Plasmin inhibitor ( alpha 2 PI ) activity in the plasma was measured using a synthetic chromogenic substrate method after incubation with jararhagin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Defects in the fibrinolytic system decreased tissue type plasminogen activator ( t PA ) activity and elevated levels of plasminogen activator inhibitor ( PAI 1 ) , in patients with SLE have been associated with an increased tendency to thrombosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The antigen levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , plasminogen activator inhibitor type 2 ( PAI 2 ) , tissue type plasminogen activator ( t PA ) , and urokinase type plasminogen activator ( u PA ) were measured during pregnancy and severe preeclampsia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In plasma samples of 38 patients ( median age : 49 years ) receiving CMF ( schedule 1 21 or 1 8 ) for Stage 2 breast cancer , we evaluated : PT , aPTT , antithrombin 3 ( AT 3 ) , protein C ( PC ) , protein S ( PS ) , thrombin antithrombin complex ( TAT ) , prothrombin fragment F 1 + 2 ( F 1 + 2 ) , fibrinogen ( Fbg ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) and D dimer ( D D ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured PAI 1 activity , tissue type plasminogen activator ( t PA ) antigen , a 2 antiplasmin , fibrinogen , lipids and apolipoproteins in both groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Urokinase type plasminogen activator ( uPA ) , uPA receptor , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , matrix metalloproteinases MMP 8 , MMP 9 and tissue inhibitor of metalloproteinases TIMP 1 were measured by ELISA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : PAI 1 activity and antigen levels were determined using a tissue type plasminogen activator ( t PA ) binding assay and Western blotting . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Accordingly , PAI 1 was recovered in a reactive centre cleaved form from incubations with urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) at 0 degree C , but not at 37 degrees C . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : The plasminogen system , which includes tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and their main inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , plays a major role in both fibrinolysis and tissue remodeling . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of matrix metalloproteinase 1 ( MMP 1 ) , matrix metalloproteinase 8 ( MMP 8 ) , matrix metalloproteinase 9 ( MMP 9 ) , lactoferrin and urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) and the inhibitors , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasminogen activator inhibitor ( PAI 2 ) , and alpha 2 macroglobulin in the synovial fluids of patients with rheumatoid arthritis was determined before and during chemical synoviorthesis with a sodium salt of the fatty acids from cod liver oil ( Varicocid ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In HUVECs , synthesis of tissue type plasminogen activator or PAI 1 was not altered by LIF or OSM ( ELISA , n = 9 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The rise in blood fibrinolysis is mainly due to an increase in tissue type plasminogen activator ( t PA ) and a decrease in its main inhibitor plasminogen activator inhibitor ( PAI 1 ) which are released from the endothelial cells of the vessel wall . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The incubation of mesangial cells with TSP increased the secretion of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , while plasminogen activator inhibitor type 1 ( PAI 1 ) decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to clarify a role of stromal cells in sex steroidal neovascularization , plasminogen activator inhibitor ( PAI ) 1 [ an inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) ] and its messenger ribonucleic acid ( mRNA ) were analysed in fibroblasts derived from uterine endometrium as a model for endometrial stromal cells under the influence of sex steroids . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human peritoneal mesothelial cells ( HMC ) play a critical role in maintaining the intraperitoneal balance between fibrinolysis and coagulation by expressing the fibrinolytic enzyme tissue type plasminogen activator ( t PA ) as well as a specific plasminogen activator inhibitor , PAI 1 , and the procoagulant protein tissue factor ( TF ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Parallel experiments with the cultured endothelial cells and BFA indicated that the fungal metabolite enhanced a rate of accumulation of plasminogen activator inhibitor 1 ( PAI 1 ) antigen , but not of tissue type plasminogen activator antigen in the medium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) , tissue type plasminogen activator ( tPA ) and von Willebrand factor ( vWF ) concentrations were measured by ELISA in the supernatant of the following cultures : endothelial cells from human umbilical vein ( HUVEC ) ; human colon cancer cells ( HRT 18 ) ; and co culture cells of HUVEC + HRT 18 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression of tissue type plasminogen activator ( t PA ) and a number of metalloproteases as well as plasminogen activator inhibitor 1 ( PAI 1 ) and tissue inhibitor of metalloproteases 1 ( TIMP 1 ) was analyzed in the central nervous system ( CNS ) of normal control and multiple sclerosis ( MS ) cases by immunohistopathology . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Renal biopsy tissue from 30 patients with MN along with specimens of normal kidney removed from six patients with renal tumors were examined for glomerular deposits of urokinase type plasminogen activator , tissue type plasminogen activator ( t PA ) , PAI 1 , VN , and fibrinogen by using immunofluorescence and immunoelectron microscopic techniques . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigen levels were significantly higher in type 4 HL than in the controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic variables , tissue type plasminogen activator , plasminogen activator inhibitor 1 and catecholamine levels were also measured . ^^^ Tissue type plasminogen activator antigen increased ( 31 % , p < 0 . 01 ) , but there was no significant increase in plasminogen activator inhibitor 1 , suggesting an overall increase in fibrinolysis on standing . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We estimated the euglobulin fibrinolytic activity ( EFA ) and , the activities and antigens of both tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , the expression of three glycoproteins that play a role in the plasminogen activator system as activators of proteolysis urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) were studied in various components of dedifferentiated chondrosarcomas of bone . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To know the effects of sex steroids on the potentials of growth , invasion , and metastasis with neovascularization of endometrial cancer , the expression of plasminogen activator inhibitor ( PAI ) 1 [ an inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) ] and its mRNA in well differentiated uterine endometrial cancer cell line Ishikawa was determined by an enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction Southern blotting ( RT PCR SB ) , respectively , under the influence of sex steroids . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and its physiological inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , are known to be synthesized by vascular endothelial cells and to play important roles in regulating the fibrinolytic activity of plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we examined the effect of 15 HPETE on the release of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) from cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary inhibitor of tissue type plasminogen activator and urokinase , is known to convert readily to a latent form by insertion of the reactive center loop into a central beta sheet . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary inhibitor of tissue type plasminogen activator and urokinase , is known to convert readily to a latent form by insertion of the reactive center loop into a central beta sheet . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Tetranectin ( TN ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor ( PAI 1 ) were quantitatively assessed ( ELISA ) in plasma of 41 patients with RA and 30 healthy subjects , alpha 2 Antiplasmin activity was assessed by the amidolytic method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Detailed analysis of the reaction products formed during the interaction between PAI 1 and its target proteinases tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) , in the presence of these monoclonal antibodies , revealed two distinct mechanisms of PAI 1 inactivation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both basal plasminogen activator inhibitor 1 antigen ( PAI 1 Ag ) and activity ( PAI 1 Act ; P < 0 . 05 ) , as well as basal and exercise induced tissue type plasminogen activator antigen ( t PA Ag ; P < 0 . 05 ) , were decreased after training . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the major physiologic inhibitor of tissue type plasminogen activator and urokinase , is abundantly expressed in atherosclerotic vascular wall . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the major physiologic inhibitor of tissue type plasminogen activator and urokinase , is abundantly expressed in atherosclerotic vascular wall . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , VN does not accelerate the association between tissue type plasminogen activator ( t PA ) and PAI 1 . ^^^ The composition of complexes between plasminogen activator inhibitor 1 , vitronectin and either thrombin or tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity was determined by tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) activities in cultured medium . 3 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) is the chief plasminogen activator in the blood , but its activity is restricted by plasminogen activating inhibitors type 1 ( PAI 1 ) and type 2 ( PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared to the controls , plasma levels of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor ( PAI ) 1 antigen , D Dimer and enhanced thrombin generation were significantly ( P < 0 . 0001 ) increased in children with the common factor 5 mutation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the patients and in 10 control subjects , plasma levels of thrombomodulin , beta thromboglobulin , D dimer , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) had previously been measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Pretreatment with rhIL 10 reduced both LPS induced activation of the fibrinolytic system ( plasma concentrations of tissue type plasminogen activator , plasmin alpha 2 antiplasmin complexes , and D dimer ) , and inhibition of fibrinolysis ( plasma levels of plasminogen activator inhibitor 1 ) , whereas posttreatment only inhibited the latter response . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrin D dimer , thrombin antithrombin complex ( TAT ) , plasmin plasmin inhibitor complex ( PPIC ) , soluble fibrin monomer ( sFM ) , prothrombin activated peptide F 1 + 2 ( F 1 + 2 ) , thrombomodulin ( TM ) , tissue type plasminogen activator ( t PA ) , and PA inhibitor ( PAI 1 ) in DIC patients were significantly higher than levels in Non DIC patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To study the hemostatic profile and endothelial cell state , we measured the blood pressure ( BP ) , hematocrit and lipid profiles as well as fibrinogen , a marker of fibrin turnover ( D dimer ) , fibrinolytic factors ( plasmin alpha 2 plasmin inhibitor complex [ PIC ] , tissue type plasminogen activator [ t PA ] antigen and t PA inhibitor [ PAI ] activity ) and an endothelial cell derived marker ( von Willebrand factor [ vWF ] ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total fibrinolytic activity , measured on fibrin plates , plasma concentration of tissue type plasminogen activator ( tPA : Ag ) and plasmin alpha 2 antiplasmin complexes ( PAP ) were low , whereas plasminogen activator inhibitor 1 concentration ( PAI 1 : Ag ) was elevated in comparison with normal values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether the T cell lymphokines interleukin 4 ( IL 4 ) and interferon gamma ( IFN gamma ) modulate SMC fibrinolysis and migration induced by basic fibroblast growth factor ( bFGF ) , we examined the effects of IL 4 and IFN gamma on human SMC tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) antigen production , determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum ACE activity and plasma fibrinolytic variables [ plasminogen activator inhibitor ( PAI ) activity , plasminogen activator inhibitor type 1 ( PAI 1 ) antigen level , and tissue type plasminogen activator ( TPA ) antigen level ] were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in healthy individuals divided by smoking habit into current smokers , former smokers and non smokers ( who had never smoked ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Wild type PAI 1 ( wtPAI 1 ) revealed the same conformational distribution pattern toward tissue type plasminogen activator ( t PA ) as toward urokinase type plasminogen activator ( u PA ) ( i . e . 53 + / 6 . 9 % active , 36 + / 6 . 8 % latent , and 12 + / 1 . 9 % substrate ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using this approach , the expression of RNAs for tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , plasminogen activator inhibitor 2 , protease nexin , and urokinase receptor isoform 1 ( uPAR 1 ) were detected in mouse osteoclasts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To elucidate the molecular interactions that underlie the interplay between the receptor , RAP , and the ligands , we have analyzed and delineated the binding sites of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The purified recombinant porcine PAI 1 ( rpoPAI 1 ) had a specific activity of 508 , 800 + / 800 U / mg ( mean + / SD , n = 3 ) towards human tissue type plasminogen activator ( ht PA ) and a functional half life in vitro of 2 . 1 + / 0 . 8 h ( n = 3 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen dependent and independent proteolytic activity of marine endothelioma ( End ) cells that were derived from mice with targeted inactivation of the tissue type plasminogen activator ( t PA / ) , urokinase type plasminogen activator ( u PA / ) or plasminogen activator inhibitor 1 ( PAI 1 / ) genes was studied with the use of fibrin and extracellular matrix degradation assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin degradation products ( FbDP ) , D dimer ( DD ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) were measured as parameters for ongoing fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Molecular modeling techniques combined with recently acquired biochemical / biophysical data were used to provide insight into the stable complex formation between plasminogen activator inhibitor 1 ( PAI 1 ) and the target proteinases : tissue type plasminogen activator , urokinase type plasminogen activator , and thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis is regulated in part by the interaction of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) antigen and activity and plasminogen activator inhibitor 1 ( PAI 1 ) antigen and activity were measured in 12 hypertensive ( 69 + / 1 yr ) and 11 normotensive ( 64 + / 1 yr ) men before and after an acute bout of submaximal exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombomodulin ( TM ) , beta thromboglobulin ( beta TG ) , D dimer ( DD ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) and quantitative determination of functional protein S ( PS ) were measured using ELISA procedures in the plasma of 16 untreated patients with newly diagnosed deep vein thrombosis in the leg and in 10 healthy volunteers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Imbalance of tissue type plasminogen activator ( t PA ) and its specific inhibitor ( PAI 1 ) in patients with rheumatoid arthritis associated with disease activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen system , via its triggers , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , has been implicated in thrombosis , arterial neointima formation , and atherosclerosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In previous studies we found that colorectal carcinomas have a marked increase of the urokinase type of plasminogen activator ( u PA ) , and the inhibitors PAI 1 and PAI 2 , whereas the tissue type plasminogen activator ( t PA ) is found to be decreased in comparison with adjacent normal mucosa . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( TPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activity and antigen levels were measured at rest and after a maximal exercise test . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) are believed to be involved in the biochemical cascade leading to extracellular matrix degradation during ovulation , the presence and possible role of urokinase type PA ( uPA ) and its receptor ( uPAR ) in follicular wall remodeling during follicular development are poorly understood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have previously reported that notoginsenoside R 1 ( NG R 1 ) increases the synthesis of tissue type plasminogen activator ( t PA ) and decreases plasminogen activator inhibitor 1 ( PAI 1 ) activity in cultured human endothelial cells from different vascular sources . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the diabetics , the levels of Lp ( a ) as well as levels of tissue type plasminogen activator ( t PA ) antigen and PAI activity were significantly higher than normal controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements of the plasma concentrations of free tissue type plasminogen activator ( t PA ) activity , tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , plasminogen ( Plg ) activity , plasminogen ( Plg ) antigen , alpha 2 antiplasmin ( alpha 2 AP ) , D dimer and fibrin degradation products ( FbDP ) were all determined and the area under the curve ( AUC ) for each of these quantities correlated with the blood loss . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients had higher Willebrand factor antigen ( vWF ) , tissue type plasminogen activator antigen ( t PA ) , plasminogen activator inhibitor activity ( PAI ) and D dimers ( D Di ) than the reference women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate the mechanism of this decrease , we studied the regulation of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( TPA ) , tissue factor ( TF ) , and tissue factor pathway inhibitor ( TFPI ) by angiotensin 2 ( Ang 2 ) in cultured rat aortic endothelial cells . ^^^ Angiotensin 2 increases plasminogen activator inhibitor 1 and tissue factor mRNA expression without changing that of tissue type plasminogen activator or tissue factor pathway inhibitor in cultured rat aortic endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cathepsins B and D reduced the antigens of tissue type plasminogen activator , and they increased both the antigens and the activity of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When HUVECs were conditioned with AS 4 , a dose ( 0 . 01 100 microg AS IV / ml ) and time dependent decrease in plasminogen activator inhibitor type 1 ( PAI 1 ) and an increase in tissue type plasminogen activator ( t PA ) synthesis were observed , which were significant from 1 microg AS IV / ml and from 12 h of incubation with 100 microg AS IV / ml . ^^^ Regulation of the fibrinolytic potential of cultured human umbilical vein endothelial cells : astragaloside 4 downregulates plasminogen activator inhibitor 1 and upregulates tissue type plasminogen activator expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At the onset of AMI , plasma levels of tissue type plasminogen activator ( t PA ) , PA inhibitor 1 ( PAI 1 ) , fibrin D dimer , thrombin antithrombin complex ( TAT ) , and plasmin plasmin inhibitor complex ( PPIC ) were significantly increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
V . ) caused a dose related increase ( maximum , 43 52 % at 30 min ) in plasma levels of factor 8 ( FVIII ) , von Willebrand factor ( vWF ) and tissue type plasminogen activator ( t PA ) , but not in levels of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis , the process to remove fibrin in tissues , is initiated by tissue type plasminogen activator ( tPA ) and suppressed by type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Impaired fibrinolysis , resulting from increased plasminogen activator inhibitor 1 ( PAI 1 ) or reduced tissue type plasminogen activator ( t PA ) plasma levels , may predispose the individual to subacute thrombosis in sepsis and inflammation . ^^^ Adenovirus mediated transfer of tissue type plasminogen activator augments thrombolysis in tissue type plasminogen activator deficient and plasminogen activator inhibitor 1 overexpressing mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the time course and localization of ovarian tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) expression during the ovulatory period in rat by RNase protection assay and in situ hybridization . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the primary cancers , PAI 2 levels correlated weakly but significantly with those of uPA and PAI 1 , but not with tissue type plasminogen activator ( tPA ) or uPA receptor ( uPAR ) levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , antioxidant vitamins had no effect on plasma PAI activity , PAI antigen , tissue type plasminogen activator activity and antigen , fibrinogen and fibrin degradation products . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary physiological inhibitor of both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary physiological inhibitor of both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study characterizes the expression of tissue type plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) in hyperplastic vein grafts and normal venous tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We examined the patterns of expression of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin in head and neck squamous cell carcinomas using immunohistochemical techniques . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Induction of tissue type plasminogen activator ( tPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) as early growth responses in rat hepatocytes in primary culture . ^^^ Early growth response with respect to tissue type plasminogen activator ( tPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) gene expression was studied in rat hepatocytes in primary culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and von Willebrand factor ( vWF ) were measured by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We sought to learn more about this variability by examining the effects of gemfibrozil and ciprofibrate on plasma levels of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and fibrinogen in primary hyperlipidaemic patients after six and twelve weeks of treatment using different assay systems for PAI 1 and fibrinogen . ^^^ Effects of gemfibrozil and ciprofibrate on plasma levels of tissue type plasminogen activator , plasminogen activator inhibitor 1 and fibrinogen in hyperlipidaemic patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Ex vivo platelet aggregation , activities and concentrations of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured before , 90 and 150 minutes after drug intake . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of different flavonoids isolated from the roots of Scutellaria baicalensis Georgi on the production of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) induced by thrombin and thrombin receptor agonist peptide , Ser Phe Leu Leu Arg Asn Pro Asn Asp Lys Tyr Glu Pro Phe , have been examined in cultured human umbilical vein endothelial cells ( HUVECs ) . ^^^ Effects of flavonoids isolated from scutellariae radix on the production of tissue type plasminogen activator and plasminogen activator inhibitor 1 induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MC were found to react with antibodies against tissue type plasminogen activator ( tPA ) and urokinase receptor ( uPAR / CD87 ) , but not with antibodies against urokinase ( uPA ) or plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Quantitative immunoreactive assays for tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in the culture media indicated that OSF ( 25 micrograms / ml ) increased the accumulation of PAI 1 antigen , but not of t PA antigen , 2 . 7 fold compared with control . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using a pregnant mare serum gonadotropin ( PMSG ) / human chorionic gonadotropin ( hCG ) induced rhesus monkey corpus luteum ( CL ) model , we have studied how urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) , are temporally expressed in CL of rhesus monkey at the luteotropic and luteolytic periods . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following endothelium derived proteins were measured as markers of endothelial cell function : von Willebrand factor ( vWf ) and von Willebrand factor propeptide ( vWf : AgII ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , cellular fibronectin ( cFN ) and thrombomodulin ( TM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Components of the coagulation ( thrombin antithrombin complexes , prothrombin fragment F 1 + 2 , activated factor 7 , and factor 7 antigen ) and fibrinolytic ( fibrin split product D dimer , plasmin plasmin inhibitor complex , tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 ) systems and markers of endothelial cell perturbation / dysfunction ( von Willebrand factor and thrombomodulin ) were measured before the start of infusion and after 2 , 6 , 12 , and 24 hours . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin lysis time , tissue type plasminogen activator and plasminogen activator inhibitor 1 ( PAI 1 ) activities , and antigens were determined before and after the venous occlusion test . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of thrombin antithrombin 3 complexes ( TAT ) , the plasmin alpha 2 antiplasmin complexes ( PAP ) , tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were measured within 48 h after admission , together with respiratory variables allowing computation of the lung injury score ( LIS ) , and pulmonary microvascular permeability [ 67Gallium transferrin pulmonary leak index ( PLI ) ] , as measures of pulmonary dysfunction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We found elevated apolipoprotein A 1 and Apo E concentrations in acromegalic patients compared to controls ( apo A 1 : 1 . 79 + / 0 . 06 vs . 1 . 46 + / 0 . 04 g / l ; p < 0 . 01 ; apo E : 98 . 35 + / 6 . 4 vs . 72 . 53 + / 3 . 38 mg / l ; p < 0 . 05 ) . 30 % of the acromegalics showed increased plasminogen activator inhibitor activity ( PAI ) while 66 % had increased tissue type plasminogen activator ( t PA ) concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was conducted to determine whether prolactin ( PRL ) suppresses gonadotrophin induced ovulation and disturbs the co ordinated gene expression of tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in rat ovary . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentrations of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and D dimer were investigated in 50 patients treated intravenously for acute myocardial infarction with either streptokinase ( n = 23 ) , urokinase ( n = 17 ) , or recombinant t PA ( rt PA , n = 10 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Various coagulation analytes including antithrombin 3 ( ATIII ) activity , protein C activity , protein S activity , alpha 2 antiplasmin activity , anticardiolipin antibodies ( aCL ) , plasminogen activator inhibitor ( PAI 1 ) activity and tissue type plasminogen activator ( tPA ) antigen were measured using citrated plasma samples from the patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serosal fibrinolysis is regulated by mesothelial release of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor types 1 and 2 ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Resting levels of plasma fibrinogen , tissue type plasminogen activator ( t PA ) antigen and activity , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and activity , and fibrin D dimer were measured in 24 healthy premenopausal women : 11 sedentary ( aged 28+ / 1 years ; Pre S ) and 13 physically active ( aged 30+ / 1 years ; Pre PA ) and in 27 healthy postmenopausal women : 14 sedentary ( aged 61+ / 1 years ; Post S ) and 13 physically active ( aged 58+ / 1 years ; Post PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recent studies demonstrated that u PA , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tissue type plasminogen activator ( t PA ) are prognostic factors in breast cancer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have investigated the expression and cellular localization of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator receptor ( uPAR ) , and plasminogen activator inhibitor 1 ( PAI 1 ) as well as plasminogen activation in rat liver regeneration by recruitment of progenitor ( oval ) cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relative distribution of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) was studied in cultured human gingival fibroblasts , healthy gingival tissues and inflamed gingival tissues by immunohistochemistry . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombomodulin levels during normal pregnancy , at delivery and in the postpartum : comparison with tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^ The increase of TM levels was correlated with those of tissue type plasminogen activator and plasminogen activator inhibitor 1 antigens . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Most of the variance in DCLT ( 68 % in men , 63 % in women ) was explained by tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To better understand the impact of two common polymorphisms of the tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) genes on the in vivo regulation of fibrinolysis , we have studied this genetic variables together with other clinical and metabolic factors in a sample of the Italian population . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the changes in the plasma levels of thrombomodulin ( TM ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PA 1 1 ) as indices of endothelial injury / activation and fibrinolysis in childhood Henoch Schnlein purpura ( HSP ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Concentrations of haemoglobin , tissue type plasminogen activator and plasminogen activator inhibitor 1 ; activated partial thromboplastin time ; prothrombin time ; platelet and reticulocyte counts ; blood loss ; and transfusions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Regulation of tissue type plasminogen activator ( tPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) gene expression in rat hepatocytes in primary culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We measured tissue type plasminogen activator ( t PA ) and PAI 1 activities in the plasma of 18 HIV infected patients with biopsy proven HUS ( HIV+ / HUS+ ) and 48 HIV infected patients without HUS ( HIV+ / HUS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma fibrinogen ( FGN ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , lipoprotein ( a ) ( Lp ( a ) ) , and net fibrinolytic activity of plasma were assayed 6 24 months after transplant as indicators of the fibrinolytic system and then correlated with the IVUS measurements . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Plasma tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) were evaluated and compared with plasma 17 beta estradiol levels , ranging from 20 pg / mL to > 5 , 000 pg / mL during the course of treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Incubation of HTC rat hepatoma cells with the cyclic nucleotide analogue , 8 bromo cAMP , causes a dramatic increase in tissue type plasminogen activator activity secondary to a 90 % decrease in PAI 1 mRNA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied the effects of PRAP 1 , a PAI 1 inhibiting polyclonal antibody , on tissue type plasminogen activator ( t PA ) induced lysis of arterial like thrombi prepared in vitro from rat and human blood in a Chandler loop device . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) and its inhibitor ( PAI 1 ) in patients treated with continuous ambulatory peritoneal dialysis . ^^^ AIM : To establish whether the values of two key enzymes of fibrinolysis , tissue type plasminogen activator ( tPA ) and its inhibitor ( PAI 1 ) , differ between patients treated with continuous ambulatory peritoneal dialysis ( CAPD ) and healthy volunteers and whether plasma and dialysate tPA and PAI 1 values vary during one exchange of dialysis solution . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate a potential physiological role of the plasminogen / plasmin system in activation of the matrix metalloproteinase ( MMP ) system , the distribution of latent and active MMP 2 ( gelatinase A ) or MMP 9 ( gelatinase B ) was monitored in aorta extracts and in serum free conditioned cell culture medium obtained from wild type ( WT ) mice and from mice with deficiency of tissue type plasminogen activator ( t PA ( / ) ) , urokinase type plasminogen activator ( u PA ( / ) ) , plasminogen activator inhibitor 1 ( PAI 1 ( / ) ) or plasminogen ( Plg ( / ) ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To understand the hormonal regulation of the components of the plasminogen plasmin system in human breast cancer , we examined the oestradiol ( E 2 ) regulation of plasminogen activators ( PAs ) , namely urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and uPA receptor ( uPAR ) , in our model system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The cultured mesothelial cells produced tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 and type 2 ( PAI 1 and PAI 2 ) during unstimulated conditions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Samples were taken before , at 3 and 9 months after the beginning of the program to measure : tissue type plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI 1 ) activity and antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was designed to investigate the effect of prolactin ( PRL ) on plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) gene expression in eCG primed granulosa cells in vitro . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The amounts of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , and the activity of u PA and t PA were determined by enzyme linked immunosorbent assay ( ELISA ) , and u PA and PAI 1 were immunolocalized using monoclonal antibodies in human brain tumours and normal brain tissues . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we determined the plasma and tissue concentrations of tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , plasminogen activator inhibitor 2 and urokinase type plasminogen activator receptor in 32 patients with pathology proved gastric cancer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) were studied in 18 smokers and 18 closely matched non smokers , all of whom had Type 1 diabetes mellitus ( DM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The increased IGFBP 3 proteolysis most probably resulted from amplified expression of tissue type plasminogen activator ( t PA ) and depression of its inhibitor ( PAI 1 ) observed in IGFBP 2 expressing xenografts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern analysis of hypoxic murine lung demonstrated an increase in PAI 1 mRNA compared with normoxic controls ; in contrast , transcripts for both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) decreased under hypoxic conditions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Samples were then assayed for von Willebrand factor ( vWf ) , tissue type plasminogen activator ( tPa ) , and plasminogen activator inhibitor ( PAI ) levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue concentrations of urokinase type plasminogen activator ( u PA ) , urokinase type plasminogen activator receptor ( u PAR ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) were investigated by an ELISA technique in normal and malignant samples of the prostate from 24 patients undergoing radical prostatectomy for organ confined prostate cancer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , plasma levels of tissue type plasminogen activator and urokinase type plasminogen activator are significantly higher in the hemolytic uremic patients than in those with acute renal failure of other causes ( P < 0 . 01 and P < 0 . 05 respectively ) . ( 4 ) Hemodialysis leads to an increase in tissue type plasminogen activator antigen and a decrease of plasminogen activator inhibitor 1 activity levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
One determinant of this system is the balance between plasminogen activators ( tissue type plasminogen activator [ t PA ] ) and inhibitors ( plasminogen activator inhibitor 1 [ PAI 1 ] ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The peptide blocked PAI 1 was a substrate for both tissue type plasminogen activator ( tPA ) and trypsin and was hydrolyzed at the scissile bond . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast to the results for PAI 1 , there was a significantly greater decrease in tissue type plasminogen activator ( tPA ) antigen in the metformin than in the placebo group ( mean+ / SD : 1 . 1+ / 3 . 1 vs . 0 . 2+ / 3 . 2 ng / ml , P < 0 . 02 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : At the time of opening of the abdominal cavity , levels of plasminogen activator inhibitor ( PAI ) type 1 ( P = 0 . 009 ) and tissue type plasminogen activator ( tPA ) / PAI complex ( P = 0 . 008 ) were increased in peritoneal samples from patients with severe adhesions compared with those in samples from patients with less severe adhesions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The aim of the present study was to test whether plasma levels of PAI 1 , tissue type plasminogen activator ( tPA ) , von Willebrand factor ( vWF ) , and thrombomodulin ( TM ) could predict the occurrence of a first acute myocardial infarction ( AMI ) in a population with high prevalence of coronary heart disease by use of a prospective nested case control design . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 contains a cryptic high affinity receptor binding site that is exposed upon complex formation with tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To test this hypothesis , we examined the effect of salt depletion on tissue type plasminogen activator ( tPA ) antigen and plasminogen activator inhibitor 1 ( PAI 1 ) activity and antigen in normotensive subjects in the presence and absence of quinapril ( 40 mg BID ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Epidemiological studies have demonstrated that levels of plasma fibrinogen , von Willebrand factor ( vWf ) , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) are associated with the incidence of vascular disease . ^^^ In vitro effect of oncostatin M on the release by endothelial cells of von Willebrand factor , tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following hemostatic parameters were studied : plasma fibrinolytic activity ( PFA ) , euglobulin fibrinolytic activity ( EFA ) , tissue type plasminogen activator ( t PA ) , fast acting plasminogen activator inhibitor 1 ( PAI 1 ) , and D dimer ( D D ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Hormone replacement therapy during 1 year caused a marginal but significant increase in plasma concentration of factor VIIc after 12 months of treatment ( P < . 05 ) , a significant decrease in fibrinogen , and a significant decrease in the protein concentrations of tissue type plasminogen activator , plasminogen activator inhibitor 1 , and lipoprotein ( a ) after 3 , 6 , and 12 months of treatment ( P < . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MC were examined by Giemsa staining and by immunohistochemistry using antibodies against tryptase , chymase , tissue type plasminogen activator ( tPA ) , urokinase ( uPA ) , urokinase receptor ( uPAR ) , and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples drawn on admission , on four occasions during operation as well as 2 hours after operation and on the first postoperative day in 50 patients undergoing elective LC were analyzed for prothrombin fragment 1+2 ( F1+2 ) , soluble fibrin ( SF ) , D dimer ( DD ) , fibrin degradation products ( FbDP ) , tissue type plasminogen activator ( tPA ) activity and antigen , and plasminogen activator inhibitor ( PAI ) activity and antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When added to confluent HUVEC , factor Xa induced the expression of tissue factor and the release of tissue type plasminogen activator and plasminogen activator inhibitor 1 without affecting urokinase expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To understand this apparent paradox of increased inhibition of fibrinolysis ( high PAI 1 ) combined with increased fibrinolytic activity ( high D dimer ) , we examined the relation between D dimer , PAI 1 and the activator of fibrinolysis , tissue type plasminogen activator ( t PA ) in subjects with varying severity of peripheral atherosclerosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Regulation of tissue type plasminogen activator and its inhibitor ( PAI 1 ) by lipopolysaccharide induced phagocytosis in a Sertoli cell line . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activities of plasmin activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) , as well as t PA antigen levels , were determined in plasma before , during and 60 min after the infusion of angiotensin 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Samples of stimulated conditioned media were collected over a period of 24 hours to determine : plasminogen activator ( PA ) and plasminogen activator inhibitor ( PAI ) activity , PAI 1 mRNA , tissue type plasminogen activator ( t PA ) antigen and urokinase type plasminogen activator ( u PA ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Definite increase in tissue type plasminogen activator ( t PA ) antigen ( p = 0 . 00075 ) with a modest elevation of its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) ( p = 0 . 27 ) probably contributes to this effect . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before and after pharmacological treatment , were evaluated the clinical symptoms ( claudicatio intermittens and rest pain ) and the following haemostatic parameters : plasma thrombomodulin ( TM ) , beta thromboglobulin ( beta TG ) , D dimer ( DD ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin fragment 1+2 ( F1+2 ) , thrombin antithrombin 3 complexes ( TAT ) , tissue type plasminogen activator ( t PA ) functional and antigen , plasminogen activator inhibitor 1 ( PAI 1 ) , plasminalpha 2 antiplasmin complexes ( PAP ) , D Dimer , thrombomodulin ( TM ) and von Willebrand factor ( vWF ) were measured in patients and in 30 healthy subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have extended this study and evaluated whether or not this was also true for the genetic polymorphisms of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , angiotensin converting enzyme ( ACE ) and angiotensinogen in a group of 133 Greenland Inuit , aged 30 34 gamma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The role of fibrinolytic system components in thrombus formation and removal in vivo was investigated in groups of six mice deficient in urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , or plasminogen activator inhibitor 1 ( PAI 1 ) ( u PA / , t PA / or PAI 1 / , respectively ) or of their wild type controls ( u PA+ / + , t PA+ / + or PAI 1+ / + ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The changes of plasma lipid peroxide ( LPO ) , superoxide dismutase ( SOD ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) and nitric oxide ( NO ) were observed in the patients treated with YQTLP and nitroglycerin , which were compared to 30 healthy subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The presence of the two plasminogen activators ( PAs ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) , and their inhibitor type 1 ( PAI 1 ) in bone cells , suggests a role in one or more aspects of bone resorption such as osteoclast formation , mineral dissolution , and degradation of the organic matrix . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A cell line , TKM 33 , has been established and cloned from human umbilical vein endothelial cells , was previously reported to produce a large amount of urokinase type PA ( u PA ) and small amounts of tissue type plasminogen activator ( t PA ) and PA inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Complexity of coronary stenosis was positively correlated with tissue type plasminogen activator ( TPA ) mass concentration ( P < 0 . 01 ) and PAI activity ( P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prior studies from our laboratory have established that decidual cells of luteal phase and pregnant endometrium express two crucial modulators of hemostasis : 1 ) tissue factor ( TF ) , the primary initiator of hemostasis via factor Xa activation ; and 2 ) plasminogen activator inhibitor type 1 ( PAI 1 ) , the fast inhibitor of the primary fibrinolytic agent , tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concerning matrix turnover proteins , simvastatin markedly reduced mRNA expression of plsminogen activator inhibitor 1 ( PAI 1 ) without affecting the expression of tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue type plasminogen activator ( t PA ) is a serine protease that is involved in neuronal plasticity and cell death induced by excitotoxins and ischemia in the brain . t PA activity in the central nervous system is regulated through the activation of serine protease inhibitors ( serpins ) such as the plasminogen activator inhibitor ( PAI 1 ) , the protease nexin 1 ( PN 1 ) , and neuroserpin ( NSP ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular endothelial cells play a critical role in the regulation of coagulation and fibrinolysis by controlling the expression of tissue factor ( TF ) , tissue factor pathway inhibitor ( TFPI ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue type plasminogen activator ( TPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) play important roles in fibrinolysis , cell migration , tissue destruction , angiogenesis and tissue remodeling . u PA and t PA activity in tissue are tightly regulated by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CRP was also associated with other variables of the insulin resistance syndrome , including blood pressure , insulin , high density lipoprotein cholesterol , triglycerides , apolipoprotein A 1 ( inversely ) , plasminogen activator inhibitor 1 antigen , and tissue type plasminogen activator antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied plasminogen activator inhibitor type 1 antigen ( PAI 1 ) , tissue type plasminogen activator antigen ( t PA ) , D dimer ( DD ) , plasmin alpha 2 antiplasmin complexes ( PAP ) , and thrombin antithrombin 3 complexes ( TAT ) in 7 children with peritonitis ( group A ) and 12 children during stable peritoneal dialysis ( group B ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thereby , human peritoneal mesothelial cells ( HMC ) play an important role in maintaining the balance between the peritoneal generation and degradation of fibrin by expressing the fibrinolytic enzyme tissue type plasminogen activator ( t PA ) as well as the specific plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Cathepsin B ( CATB ) and cathepsin L ( CATL ) , which are cysteine proteases , urokinase ( UPA ) and tissue type plasminogen activator ( TPA ) , both serine proteases , and their inhibitor type 1 ( PAI 1 ) are believed to play an important role in colorectal carcinoma ( CRC ) invasion and metastasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen concentrations were measured by ELISA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The mediators of the plasminogen activator system , namely urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , are a key complex involved in fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Local insulin infusion stimulates expression of plasminogen activator inhibitor 1 and tissue type plasminogen activator in normal subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using surface plasmon resonance , we studied the binding of these fragments to methylamine activated alpha ( 2 ) macroglobulin , pro urokinase type plasminogen activator , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 , t PA . plasminogen activator inhibitor 1 complexes , lipoprotein lipase , apolipoprotein E , tissue factor pathway inhibitor , lactoferrin , the light chain of blood coagulation factor 8 , and the intracellular chaperone receptor associated protein ( RAP ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Substitution of the native variable region 1 ( VR1 / 37 loop ) of thrombin by the corresponding VR 1 of tissue type plasminogen activator ( thrombin VR 1 ( tPA ) ) increases the rate of inhibition by plasminogen activator inhibitor type 1 ( PAI 1 ) by three orders of magnitude , and is thus sufficient to confer PAI 1 specificity to a heterologous serine protease . ^^^ The variable region 1 from tissue type plasminogen activator confers specificity for plasminogen activator inhibitor 1 to thrombin by facilitating catalysis : release of a kinetic block by a heterologous protein surface loop . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PAI 1 mutants His143Asp and His143Phe only displayed about 20 % of the specific activity obtained for wtPAI 1 , because they , to a large extent , act as substrates for tissue type plasminogen activator . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVES : To measure tumor necrosis factor alpha ( TNF alpha ) and interleukin 1beta ( IL 1beta ) in pleural effusions caused by tuberculosis ( TB ) and malignancy and their relationship with plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) , and to compare the differences between tuberculous and malignant pleural effusions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cell conditioned medium was assayed for tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator urokinase type plasminogen activator and plasminogen activator inhibitor 1 mRNA were expressed in glioma cells during the entire tumor growth . ^^^ Our finding may suggest a functional co operation of tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 and vascular endothelial growth factor during glioma progression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we investigated the effects of angiotensin metabolites on the mRNA expression of tissue factor ( TF ) , tissue factor pathway inhibitor ( TFPI ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue type plasminogen activator ( TPA ) in cultured rat aortic endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recent studies suggest that high plasma levels of tissue type plasminogen activator ( tPA ) and its inhibitor ( plasminogen activator inhibitor 1 , PAI 1 ) are markers of an increased risk of atherothrombotic ischemic events such as stroke and myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In the present study , we determined the plasma concentrations of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , PAI 2 , and uPA receptor ( uPAR ) in 25 patients with ovarian cancer , 16 patients with benign gynecologic tumor or inflammation , and 36 healthy controls in order to find out whether the plasma levels of these markers could be used to evaluate the prognostic value in patients with gynecologic cancers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Trends across quintiles of fasting insulin in levels of plasminogen activator inhibitor 1 ( PAI 1 ) antigen , tissue type plasminogen activator ( tPA ) antigen , von Willebrand factor ( vWF ) antigen , factor 7 antigen , fibrinogen , and plasma viscosity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activator inhibitor ( PAI ) 1 , tissue type plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) were investigated in the pre surgical period and in the postoperative follow up period in children suffering from Ewing sarcoma ( ES ; n = 36 ) or osteosarcoma ( OS ; n = 39 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples for coagulation studies ( fibrinogen , von Willebrand factor ( vWf ) , factor 8 : C , antithrombin , protein C , plasminogen , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI ) , D dimer ) were obtained within two hours of admission , 24 h later , and on days 3 5 , 7 9 , 21 and 35 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NO can also affect the fibrinolytic activity by regulating the release of tissue type plasminogen activator and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined whether statins altered expression of the major determinants of fibrinolytic balance , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue type plasminogen activator ( tPA ) in human vascular smooth muscle ( SMC ) and endothelial ( EC ) cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antigen levels of tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) were comparable with young endothelial cells , and mRNA was present for tPA , PAI 1 , tissue factor ( TF ) , tissue factor pathway inhibitor and thrombomodulin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We investigated 20 patients ( M / F = 15 / 5 ; mean age = 36 . 7 years ) with angiographically proven CAD for their plasma levels of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) at basal conditions as well as after VO and at maximal ES ( standardised bicycle stress test ) and compared the data to those obtained from 12 sex and age matched healthy controls ( M / F = 9 / 3 ; mean age = 40 . 4 years ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The temporal profiles of the expression of these genes were different from those of other angiogenic genes such as basic fibrobast growth factor / fibroblast growth factor receptor and vascular endothelial growth factor / vascular endothelial growth factor receptor and proteolytic enzymes ( tissue type plasminogen activator and urokinase plasminogen activator ) and their inhibitors ( plasminogen activator inhibitor 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements of fasting triglycerides and antigen concentrations of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were also included . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The low density lipoprotein receptor related protein ( LRP ) is a multiligand clearance receptor that removes free tissue type plasminogen activator ( t PA ) or complexes of t PA with plasminogen activator inhibitor type 1 ( PAI 1 ) from the blood circulation or the pericellular space . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After 4 weeks ' dietary intervention fasting plasma factor 7 coagulant ( FVIIc ) activity , tissue type plasminogen activator ( t PA ) activity , plasminogen activator inhibitor ( PAI 1 ) antigen and beta thromboglobulin did not differ between diets M and D . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the relationships of plasma tissue type plasminogen activator ( tPA ) and PAI 1 antigen levels , plasma PAI 1 activity and PAI 4 / 5 guanosine ( 4G / 5G ) genotype to CAD progression in 203 middle aged men participating in the Lopid Coronary Angiography Trial ( LOCAT ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TNK tissue plasminogen activator ( TNK t PA ) , a bioengineered variant of tissue type plasminogen activator ( t PA ) , has a longer half life than t PA because the glycosylation site at amino acid 117 ( N117Q , abbreviated N ) has been shifted to amino acid 103 ( T103N , abbreviated T ) and is resistant to inactivation by plasminogen activator inhibitor 1 because of a tetra alanine substitution in the protease domain ( K296A / H297A / R298A / R299A , abbreviated K ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) , the primary inhibitor of tissue type plasminogen activator ( t PA ) , circulates as a complex with the abundant plasma glycoprotein , vitronectin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In human endothelial cells , PAI 1 was diminished by 32 % ( P < 0 . 001 ) , whereas tissue type plasminogen activator was unaffected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( s ) : Expression of the messenger ribonucleic acid ( mRNA ) of tissue type plasminogen activator ( t PA ) and that of a specific PA inhibitor type 1 ( PAI 1 ) in endometrial tissue samples , as evaluated with the use of Northern blot analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The increase in fibrinolysis is due to a rise in tissue type plasminogen activator ( tPA ) and decrease in plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular endothelial cells ( ECs ) modulate the blood fibrinolytic system by secreting tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and their inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) and lipoprotein ( a ) [ Lp ( a ) ] in 133 healthy subjects were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Yet , studies associating myocardial infarction with gene polymorphisms of vascular proteins ( angiotensinogen , angiotensin converting enzyme , angiotensin 2 type 1 receptor , endothelial nitric oxide synthase ) and haemostatic factors ( fibrinogen , coagulation factors 2 , 5 , 7 and 13 , plasminogen activator inhibitor 1 , tissue type plasminogen activator , platelet glycoproteins IIb / IIIa , Ia / IIa and Ib 9 5 , or methylenetetrahydrofolate reductase ) have revealed conflicting results . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although the levels of von Willebrand factor and plasminogen activator inhibitor 1 showed essentially the same changes in both groups during and after percutaneous transluminal coronary angioplasty , more markedly increased levels of tissue type plasminogen activator and type plasminogen activator plasminogen activator inhibitor 1 complex after percutaneous transluminal coronary angioplasty were found in group 1 than in group 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples for endotoxin , plasminogen activator inhibitor 1 ( PAI 1 ) activity , tissue type plasminogen activator ( t PA ) antigen , and cross linked fibrin degradation products ( XL FDP ) were drawn before , during , and 7 days after surgery . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
ELISA for tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in the culture media indicated that OSF increased the bFGF induced release of PAI 1 antigen , but not of t PA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Proteins influencing plasminogen activation to plasmin , namely plasminogen activators tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and their principal inhibitors , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 , were measured in the plasma , the polymorph and mononuclear cell fractions taken from patients with major sepsis who were entering a general intensive care unit . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study demonstrated that glycation enhanced Lp ( a ) induced production of plasminogen activator inhibitor 1 ( PAI 1 ) , and further decreased the generation of tissue type plasminogen activator ( t PA ) from human umbilical vein endothelial cells ( HUVEC ) and human coronary artery EC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to elucidate the relationship between homocysteine and the fibrinolytic system , we examined the effect of homocysteine on plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) gene expression and protein secretion in cultured human vascular endothelial and smooth muscle cells in vitro . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue type plasminogen activator activity peaked at 1 . 5 hours ( 11 . 3 fold increase ) , whereas the peak of plasminogen activator inhibitor 1 activity ( 14 fold increase at 4 . 5 hours ) was delayed compared to tissue plasminogen activator and completely extinguished plasma tissue plasminogen activator activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied whether body fatness , leptin , and insulin interact with plasminogen activator inhibitor 1 antigen ( PAI 1 Ag ) and tissue type plasminogen activator antigen ( tPA Ag ) in obese children and adolescents . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , EPA E significantly and dose dependently ameliorated coagulation related parameters , including the prothrombin time ( PT ) , activated partial thromboplastin time ( APTT ) , fibrinogen level , and factor 2 , 5 , 7 , 8 , 9 , 10 , 11 , and 12 and antithrombin 3 ( AT 3 ) activities , and fibrinolysis related parameters , including plasminogen , tissue type plasminogen activator ( t PA ) , alpha 2 plasmin inhibitor ( alpha 2 PI ) , and plasminogen activator inhibitor ( PAI ) , and also suppressed ADP or collagen induced platelet aggregation and the cholesterol to phospholipid ( C / P ) molar ratio in platelet membranes at a dose of 0 . 1 g / kg or higher . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fluvastatin inhibits basal and stimulated plasminogen activator inhibitor 1 , but induces tissue type plasminogen activator in cultured human endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of : von Willebrand Factor ( vWF ) , thrombomodulin , plasminogen activity inhibitor type 1 ( PAI 1 : antigen and activity ) , tissue type plasminogen activator ( t PA : antigen ) , prothrombin fragment 1+2 ( F1+2 ) and thrombin antithrombin complexes ( TAT ) were measured previously to CRRT ( base line ) , and after 24 and 48 hours of therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At an enzyme / substrate ratio of 1 / 10 at 37 degrees C , PAI 1 protein cleavage occurred with half lives of 27 or 14 min for active or stable PAI 1 and was associated with rapid loss of inhibitory activity toward tissue type plasminogen activator with half lives of 15 or 13 min , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The functional significance of PAI 1 secretion was demonstrated by complete inhibition of tissue type plasminogen activator activity with supernatants of stimulated HMC 1 cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activation potential in the blood is controlled by an equilibrium between plasminogen activators , mainly tissue type plasminogen activator ( t PA ) , and inhibitors , mainly plasminogen activator inhibitor ( PAI ) 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We therefore investigated the effects of atorvastatin ( ATOR ) and fluvastatin ( FLU ) on PAI 1 and also tissue type plasminogen activator ( t PA ) synthesis in 20 % fetal calf serum cultured human umbilical vein endothelial cells ( HUVEC ) stimulated or not by recombinant human pro inflammatory cytokines , i . e . tumor necrosis factor alpha ( TNFalpha ) and interleukin 1alpha ( IL 1alpha ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Job strain was measured with the Job Content Questionnaire ; CVD risk was measured with insulin , total cholesterol , triglyceride ( TG ) , high density lipoprotein cholesterol ( HDL C ) , fibrinogen , tissue type plasminogen activator ( tPA ) antigen , tPA activity , plasminogen activator inhibitor 1 antigen , and blood pressure . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In 20 patients with RP ( in all patients secondary to SSc ) and in 10 controls the levels of thrombomodulin ( TM ) , beta thromboglobulin ( beta TG ) , D dimer ( DD ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) were measured in venous plasma samples taken at 9 . 00 and 14 . 00 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Peripheral blood was taken perioperatively and PAI 1 , tissue type plasminogen activator ( tPA ) , plasma aspartate aminotransferase ( AST ) and cytokine levels were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : As estimated from the measurements on placebo , type 1 diabetic patients , as compared to the controls , had some impairment of endothelial function : plasma tissue type plasminogen activator levels were lower ( 3 . 5 vs . 5 . 4 ng mL ( 1 ) , P < 0 . 05 ) , but there were no significant differences in brachial artery flow mediated vasodilation or plasma levels of von Willebrand Factor , endothelin 1 , plasminogen activator inhibitor 1 , soluble E selectin or vascular cell adhesion molecule 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since alpha ( s 2 ) casein also binds plasminogen and tissue type plasminogen activator ( t PA ) , resulting in the conversion of plasminogen to plasmin , and since IGFBP 5 binds to plasminogen activator inhibitor 1 ( PAI 1 ) , we investigated whether apoptosis and extracellular matrix ( ECM ) degradation might be coordinately controlled by GH and PRL possibly acting through IGFBP 5 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The expression of uPA , uPAR , the tissue type plasminogen activator , and plasminogen activator inhibitor ( PAI ) 1 and PAI 2 was investigated using reverse transcription followed by polymerase chain reaction and Western blotting . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 6 , IL 10 , cICAM 1 , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and D dimer antigens were measured by ELISA in the blood of 45 RA patients and 33 healthy subjects ( HS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study underscores a regulatory role of intracellular ceramide in astrocytes for the release of an extracellular serine protease , tissue type plasminogen activator ( t PA ) , and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study found that genetic deficiency for PAI 1 , the primary physiologic regulator of tissue type plasminogen activator ( tPA ) , prolonged the time to occlusive thrombosis following photochemical injury to carotid atherosclerotic plaque in apolipoprotein E deficient ( apoE ( / ) ) mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study used two mutants of tissue type plasminogen activator ( t PA ) with resistance to inhibitors of fibrinolysis to define the contribution of plasminogen activator inhibitor ( PAI ) 1 and alpha 2 antiplasmin ( alpha 2 AP ) to the control of fibrin lysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , the fibrinolytic parameters tissue type plasminogen activator ( tPA ; antigen and activity ) and plasminogen activator inhibitor type 1 ( PAI 1 ; antigen and activity ) were measured in peritoneal fluid , in peritoneal biopsies and in plasma to establish the time course of changes in fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 167 patients , undergoing PTCA , Lp ( a ) plasma levels , aCL , euglobulin lysis time ( ELT ) , plasminogen activator inhibitor 1 ( PAI 1 ) activity and tissue type plasminogen activator ( t PA ) plasma levels were evaluated before the procedure . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Factor 7 coagulant activity ( VIIc ) and factor 8 coagulant activity ( VIIIc ) were determined using one stage clotting assays ; fibrinogen was measured according to the method of Clauss ; von Willebrand factor antigen ( vWF Ag ) , tissue type plasminogen activator antigen ( tPA Ag ) , and plasminogen activator inhibitor 1 antigen ( PAI 1 Ag ) were determined by ELISA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When compared to patients with benign breast conditions there was a far greater plasma level of factor 8 vWF , fibrinogen , thrombin antithrombin 3 complex , D dimer fibrin degradation products , tissue type plasminogen activator and the activity and the antigen of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied thrombin antithrombin complexes ( TAT ) , prothrombin fragments 1+2 ( F1+2 ) , tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) concentrations in patients with acute middle cerebral artery ischemic stroke due to atherosclerotic large artery disease ( n=50 ) , lone AF ( n=24 ) and cardioembolism ( n=21 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These cells showed reduced invasiveness that paralleled decreased mRNA levels of urokinase type plasminogen activator and its receptor , tissue type plasminogen activator , and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , the PAI 1 activity toward urokinase type plasminogen activator and tissue type plasminogen activator was significantly prolonged in the presence of alpha ( 1 ) acid glycoprotein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MCs reacted with monoclonal antibodies to tryptase , chymase , and c kit / CD117 and stained positively for tissue type plasminogen activator ( tPA ) and urokinase receptor ( uPAR / CD87 ) but did not express detectable urokinase ( uPA ) or plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to determine a correlation of cell cycle phases with the fibrinolytic system , we investigated the expression of u PA , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in normal and tumor containing prostate extracts and analyzed a possible relationship with flow cytometry determined proliferative activity of the samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the role of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) in children with an estimated risk of vascular occlusion reported to range from 7 % to 16 % , we conducted a prospective study in infants and children with underlying cardiac disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIMS : Weak staining for urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , or plasminogen activator inhibitor 1 ( PAI 1 ) confined to crescents has been described in a few cases of severe crescentic glomerulonephritis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunohistochemical analyses of the healing tissues and an analysis of the periodontal wound healing fluid by ELISA were carried out for the detection of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and 2 plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We also studied fibrinolysis parameters : plasminogen , tissue type plasminogen activator ( t PA ) antigen , t PA activity , type 1 plasminogen activator inhibitor ( PAI 1 ) antigen , PAI 1 activity , and the 4G / 5G polymorphism of the promoter region of the PAI 1 gene . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 82 subjects ( 47 free of clinical signs of CAD and in 35 survivors of AMI ) , measurements of body mass index ( BMI ) , lipoproteins , glucose , insulin , plasminogen activator inhibitor 1 , tissue type plasminogen activator ( t PA ) , euglobulin clot lysis time , fibrinogen , and platelet count were performed twice in the cold months ( December and March ) and twice in the warm months ( June and September ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the contribution of an imbalance between coagulation activation and fibinolysis activation and inhibition to morbidity and mortality in sepsis , we determined in medical hospitalized patients at inclusion ( day 0 ) for fever ( temperature above 38 . 0 degrees C axillary or 38 . 3 degrees C rectally ) , and daily thereafter for two days , circulating thrombin antithrombin 3 ( TAT ) complexes , plasmin alpha 2 antiplasmin ( PAP ) complexes ( day 0 only ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and interleukin ( IL ) 6 , the latter as a marker of the inflammatory host response . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) , fibrinogen , thrombin antithrombin ( TAT ) complexes , and prothrombin fragments ( F1 . 2 ) were measured at baseline and after standardized venous occlusion ( VO ) in patients with PPH ( 24 female , 9 male ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To clarify the role of plasminogen activator inhibitor type 1 ( PAI 1 ) in postburn hypercoagulation , we assayed the plasma levels of tissue type plasminogen activator ( t PA ) antigen , total PAI 1 antigen , and total t PA PAI 1 complex in 15 burned patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After dietary intervention decreases in blood pressure , low density lipoprotein to high density lipoprotein cholesterol ratio , triglycerides , fibrinogen , plasminogen activator inhibitor 1 , and an increase in tissue type plasminogen activator activity levels were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we characterized the production of fibrinolytic factors including tissue type plasminogen activator ( tPA ) , urokinase type PA ( uPA ) , and PAI 1 in the differentiation of preadipocytes , and examined the hormonal regulation of these fibrinolytic factors in mature adipocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , the clot dissolving efficacy of fibrinolytic agents such as tissue type plasminogen activator ( t PA ) is limited , in vivo , in part by the action of plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We used primary human vascular smooth muscle cells ( VSMC ) and studied the effects of losartan on angiotensin 2 ( Ang 2 ) mediated ( a ) DNA synthesis , ( b ) secretion of tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , ( c ) secretion of matrix metalloprotease 2 ( MMP 2 ) activity and tissue inhibitors of MMPs ( TIMPs ) , and ( d ) synthesis of glycosaminoglycans . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Disclosure of a specific prothrombotic / antifibrinolytic state by determination of plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) , and t PA / PAI 1 complex levels in plasma may help to define patients at high risk of developing acute coronary syndromes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator , tissue type plasminogen activator , urokinase type plasminogen activator receptor , and plasminogen activator inhibitor 1 expression were studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression of tissue factor ( TF ) , the primary initiator of hemostasis ; urokinase type plasminogen activator ( uPA ) ; tissue type plasminogen activator ( tPA ) ; plasminogen activator inhibitor 1 ( PAI 1 ) as well as the potent matrix metalloprotease , MMP 3 was assessed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) , the primary inhibitor of tissue type plasminogen activator ( t PA ) , is found in plasma and platelets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Because fibrinolytic system components have been demonstrated to play an important role in the balance of intraperitoneal generation and degradation of fibrin , we studied the effect of early and advanced glycated human serum albumin , methylglyoxal , and 3 deoxyglucosone on the synthesis of tissue type plasminogen activator ( tPA ) , as well as its specific inhibitor ( PAI 1 ) , in human peritoneal mesothelial cells ( HPMC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate the role of active plasminogen activator inhibitor 1 ( PAI 1 ) in the evolution of a microthrombus generated in the arteriolar microcirculation , the monoclonal antibody , 33H1F7 , which transforms active PAI 1 to a tissue type plasminogen activator ( t PA ) substrate , was evaluated in an arteriolar thrombosis model in the rat mesentery . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To observe the effect of Yimai Jiangya extract ( YMJYE ) on platelet alpha granule membrane protein ( GMP 140 ) , tissue type plasminogen activator ( t PA ) and its inhibitor ( PAI ) , angiotensin 2 ( AngII ) in aged patients with essential hypertension ( EH ) of stage 2 caused by Qi deficiency and blood stasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) in plasma and in human peripheral blood monocyte cell ( PBMC ) , as well as the procoagulant activity ( PCA ) in PBMC were measured by chromogenic substrate method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The purpose of this study was to determine whether plasminogen activator inhibitor 1 and tissue type plasminogen activator are upregulated after intracerebral hemorrhage and the role that thrombin plays in that induction . ^^^ In conclusion , intracerebral hemorrhage and thrombin infusion stimulate plasminogen activator inhibitor 1 but not tissue type plasminogen activator production in the brain . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels or presence of protein C , protein S , antithrombin , methylenetetrahydrofolate reductase C677T , factor 5 Leiden , prothrombin gene G20210A , antiphospholipid antibodies , plasminogen , tissue type plasminogen activator ( tPA ) , type 1 tPA inhibitor ( PAI 1 ) , PAI 1 4G / 5G polymorphism , prothrombin fragment 1+2 , plasmin / alpha ( 2 ) antiplasmin complexes , thrombomodulin , and activated factors 7 and 12 were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have examined expression of the components of the plasminogen activation system in human astrocytoma U 373 MG cells and found that interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha TNF alpha ) , interferon gamma ( INF gamma ) and epidermal growth factor ( EGF ) specifically regulate the expression of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and protease nexin 1 ( PN 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined the alteration of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) in kidneys with unilateral ureteral obstruction in rats . ^^^ Plasminogen activator inhibitor 1 and tissue type plasminogen activator are up regulated during unilateral ureteral obstruction in adult rats . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hemodynamic parameters , PAI 1 and tissue type plasminogen activator ( t PA ) antigen , potassium , PRA , angiotensin 2 , and aldosterone were measured in nine male hypertensive subjects after a 3 wk washout , after 2 wk of hydrochlorothiazide ( HCTZ ; 25 mg plus 20 mmol KCl / d ) , and after 2 wk of spironolactone ( 100 mg / d plus KCl placebo ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have previously shown that BFD patients had significant lower tissue type plasminogen activator ( t PA ) antigen level and higher plasminogen activator inhibitor , Type 1 ( PAI 1 ) antigen level than normal controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , and tPA / PAI complex in tissue extracts were determinated by commercially available enzyme linked immunosorbent assay kits . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following variables were measured before treatment as well as after 1 , 3 and 6 months of treatment : factor 7 activity , protein C activity , fibrinogen level , antithrombin 3 activity , plasminogen activator inhibitor 1 ( PAI 1 ) level and the plasma concentration of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Fibrinolytic measurements included euglobulin clot lysis time ( ELT ) , fibrinogen , plasminogen activator inhibitor 1 , and tissue type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Relationship of plasma activities of tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) with age , sex , body weight , smoking , blood lipids were studied in 131 healthy subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , menstrual and reproductive hormone patterns , lipid and glucose metabolic parameters , bilateral ovarian volume , side effects were determined before and after oral administration of metformin 375 mg three times daily for 12 16 weeks . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Other factors in patients with type 2 diabetes include alterations in serum fibrinogen , PAI 1 , tissue type plasminogen activator ( tPa ) and factors 5 , 2 and 7 , which have all been linked to the risk of myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We evaluated the two key components of the fibrinolytic system , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in women with clinical signs of unstable coronary artery disease ( CAD ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , the effects of angiotensin 2 and its receptors on the expression and release of PAI 1 and tissue type plasminogen activator ( t PA ) were examined in human coronary artery endothelial cells ( HCAECs ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we compared the temporal changes in systolic blood pressure and coronary perivascular fibrosis induced by long term treatment with N ( omega ) nitro L arginine methyl ester ( L NAME ) in wild type ( WT ) , PAI 1 ( / ) and tissue type plasminogen activator deficient ( t PA / ) mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma Lp ( a ) concentration and activation of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were determined in 50 patients with chronic renal failure ( CRF ) and in 50 healthy subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study revealed OSM mediated expression of mRNAs encoding tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Oncostatin M induces tissue type plasminogen activator and plasminogen activator inhibitor 1 in Calu 1 lung carcinoma cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have recently shown that resting human mast cells ( MCs ) produce tissue type plasminogen activator ( t PA ) without simultaneously expressing plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombus formation and degradation is partly due to a complex interplay between tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared with controls , PPH patients had elevated plasma levels of tissue type plasminogen activator ( 15 . 6 + / 9 . 9 versus 5 . 5 + / 3 ng / ml ) and plasminogen activator inhibitor 1 ( 27 . 8 + / 23 versus 16 . 4 + / 12 ng / ml ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of both tissue type plasminogen activator and urokinase type plasminogen activator in plasma , is a well established risk factor in thrombotic diseases . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of both tissue type plasminogen activator and urokinase type plasminogen activator in plasma , is a well established risk factor in thrombotic diseases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The effects of BK on the expression of tissue factor ( TF ) , tissue factor pathway inhibitor ( TFPI ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue type plasminogen activator ( TPA ) in cultured rat aortic endothelial cells ( RAECs ) were respectively evaluated by Northern blot and chromogenic assay or enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasmatic levels of the following haemostatic factors were measured within the first 6 h of the SIRS evolution : von Willebrand Factor ( vWF ) , thrombomodulin , plasminogen activity inhibitor type 1 ( PAI 1 antigen ) , tissue type plasminogen activator ( t PA antigen ) , prothrombin fragment 1 + 2 ( F 1 + 2 ) , thrombin antithrombin complexes ( TAT ) and D dimer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : The prognostic value of plasma concentrations of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and C reactive protein has been reported in patients with coronary artery disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Its major components are urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) , plasminogen activation inhibitor type 1 and 2 ( PAI 1 and PAI 2 ) and a receptor for urokinase ( uPAR ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The serum concentrations of fasting glucose , insulin , lipids , the activities of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , and the level of blood pressure were measured in 159 patients with stroke and 40 healthy control subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis , which is strictly regulated by plasminogen activator inhibitor 1 ( PAL 1 ) and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum levels of tissue type plasminogen activator ( tPA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) activity , tPA antigen , PAI 1 antigen , FVII , FX , and fibrinogen were assayed after both treatments . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
By expressing the fibrinolytic enzyme tissue type plasminogen activator ( t PA ) and its specific inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , human peritoneal mesothelial cells ( HMC ) play an important role in regulating peritoneal fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 78 non diabetic , normotensive first degree relatives of type 2 diabetic patients ( 47 without a family history of hypertension and 31 with a family history of hypertension ) and in 36 normoglycemic , normotensive subjects with no family history of hypertension , we evaluated plasma levels of fasting glucose and insulin , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , D dimer ( DD ) and prothrombin fragment 1 + 2 ( F1+2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Sixteen DM2HD patients and 23 healthy individuals ( HI ) had their euglobulin clot lysis time ( ECLT ) , and tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activities ( act ) and concentrations ( ag ) assessed before and after standard fibrinolytic stimulus ( i . v . administration of 0 . 4 microg / kg BW 1 deamino 8 D arginine vasopressin , DDAVP ) along with metabolic status markers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have compared the effects of maspin with those of PAI 1 in a range of situations in which plasminogen activation is potentiated , reflecting the biological context of this proteolytic system : urokinase type plasminogen activator bound to its receptor on the surface of tumor cells , tissue type plasminogen activator specifically bound to vascular smooth muscle cells , fibrin , and the prion protein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We used electron microscopy and immunoconfocal ( IF ) labeling for bFGF , matrix metalloproteinase ( MMP ) 2 , MMP 9 , tissue type plasminogen activator ( tPA ) , its inhibitor ( PAI 1 ) , fibronectin ( FN ) , and Ki 67 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue concentrations of tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and the resulting tPA activity were assayed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 100 healthy individuals and 129 patients with venous thromboembolism , we have determined antigenic FVIII levels along with several endothelial proteins including vWF , soluble thrombomodulin ( sTM ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The parameters examined were tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hydrochlorothiazide increased plasma PAI 1 ( P=0 . 013 ) but not tissue type plasminogen activator ( tPA ) ( P=0 . 431 ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After 6 months of HRT , significant decreases in activity of antithrombin 3 and protein C and levels of plasminogen activator inhibitor 1 antigen , tissue type plasminogen activator antigen , and euglobulin clot lysis time and a significant increase in D dimer level were found compared with placebo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI ) 1 , tissue type plasminogen activator activity and plasmin antiplasmin complexes ( PAP ) were determined at baseline and after the venous occlusion test . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHOD : Platelet alphagranule membrane protein ( GMP 140 ) , von Willebrand Factor ( vWF ) , antithrombin 3 activity ( AT 3 ) , protein C dependent partial thromboplastin activation time , plasminogen activity ( PLG ) , the activity of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) , D Dimer were examined in 20 normal non pregnant women and 38 patients after operation with gynecological malignancies . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Of interest , after stimulating the fibrinolytic system with the DSE test , plasminogen activator inhibitor 1 activity was significantly higher in patients with necrotic / stunned myocardium than in patients with resting normal wall motion ( P = 0 . 03 ) , whereas tissue type plasminogen activator activity after the DSE test was significantly lower in patients with stunned / necrotic myocardium than in patients with normal wall motion ( P = 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of haemostasis factors ( HFs ) such as fibrinogen , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and D dimer may be markers of arteriosclerosis for the following reasons : There seems to be no difference in levels of HFs between patients with longstanding stable angina and those with an isolated myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In fibrinolysis , a clear reduction in plasminogen activator inhibitor 1 ( PAI 1 ) was observed , but no significant change in tissue type plasminogen activator antigen ( t PA Ag ) or activity was found , although fibrinolytic activity assessed as t PA activity ( t PA Act ) tended to increase after oERT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN : We performed a placebo controlled 2 year trial among 158 healthy siblings of patients with premature atherosclerotic disease to determine the effect of daily folic acid ( 5 mg ) plus vitamin B 6 ( 250 mg ) treatment as compared with placebo medication ( n = 80 ) on markers of endothelial function ( urinary albumin to creatinine ratio and plasma concentrations of soluble E selectin , soluble vascular cell adhesion molecule 1 , von Willebrand factor , tissue type plasminogen activator and plasminogen activator inhibitor 1 ) and inflammation ( C reactive protein ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Morphometric analysis of aorta was also performed and plasminogen activator inhibitor 1 ( PAI 1 ) as well as tissue type plasminogen activator ( t PA ) plasma activities were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured markers of endothelial function [ von Willebrand factor ( vWf ) , soluble intercellular adhesion molecule 1 ( sICAM 1 ) , soluble vascular cell adhesion molecule 1 ( sVCAM 1 ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and E selectin ( ES ) ] , and markers of inflammatory activity [ secretory phospholipase A ( 2 ) ( sPLA ( 2 ) ) and C reactive protein ( CRP ) ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following were assayed : euglobulin lysis time , tissue type plasminogen activator antigen , and PAI 1 antigen and activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHOD : Plasma was obtained from 29 patients with SSc and tested for thrombin antithrombin ( TAT ) , fragments 1+2 ( F1+2 ) , dermatansulphate ( DS ) , thrombomodulin ( TM ) , lipoprotein ( a ) [ Lp ( a ) ] , von Willebrand factor ( vWF ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , D dimers , intercellular adhesion molecole 1 ( ICAM 1 ) , vascular cell adhesion molecule ( VCAM ) , and E selectin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
STUDY DESIGN : The supernatants of Porphyromonas endodontalis , Porphyromonas gingivalis , and Prevotella intermedia were used to evaluate tissue type plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) gene expression in human pulp and osteoblastic cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Concentrations of total and active transforming growth factor beta 1 ( TGF beta 1 ) , tumor necrosis factor alpha ( TNF alpha ) , tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were obtained . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human peritoneal mesothelial cells ( HMC ) play a critical role in maintaining the intraperitoneal balance between fibrinolysis and coagulation by expressing the fibrinolytic enzyme tissue type plasminogen activator ( t PA ) and its specific inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) as well as the procoagulant protein , tissue factor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : To examine the platelet alphagranule membrane protein ( GMP 140 ) , Von Willebrand factor ( vWF ) , antithrombin 3 activity ( AT 3 ) , plasminogen activity ( PLG ) , the activity of tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) in 30 non pregnancy women , 30 normal late pregnancy women and 50 pregnancy induced hypertension women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A random sample of 70 of the 112 cases had plasma levels of urokinase like plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , macrophage inhibiting factor ( MIF ) , tumour growth factor beta 1 ( TGF beta 1 ) , homocysteine , and serum levels of IgA antibodies against Chlamydia pneumoniae ( IgA CP ) and Cotinine ( a nicotine metabolite ) measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Samples of cell culture media were taken at 0 , 5 , and 24 h after exposure to measure media pH , PAI 1 , and tissue type plasminogen activator ( t PA ) protein release . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : To quantify the ex vivo production of proangiogenic proteins ( vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( tPA ) ) and angiogenesis inhibitors ( plasminogen activator inhibitor type 1 ( PAI 1 ) and angiostatin ) from epithelial and stromal components of primary prostate cancer ( CaP ) and benign prostatic hyperplasia ( BPH ) cultures . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For testing fibrinolysis , determinations of plasmin alpha ( 2 ) antiplasmin complex ( PAP ) , tissue type plasminogen activator ( tPA ) antigen , plasminogen activator inhibitor ( PAI ) 1 antigen and D dimer were used . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Likewise , atherosclerotic rabbits had reduced plasma tissue type plasminogen activator activity and D dimer levels and an increase in plasminogen activator inhibitor 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Specificity of PAI 1 is mainly determined by its reactive site ( Arg 346 Met347 ) , which interacts with serine residue of tissue type plasminogen activator ( tPA ) with concomitant formation of SDS stable complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the role of plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase plasminogen activator ( uPA ) , and tissue type plasminogen activator ( tPA ) in adhesion formation after laparoscopic surgery . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Baseline procoagulability scores and procoagulability scores in response to a 15 min speech task included plasma thrombin / antithrombin 3 complex , D dimer , von Willebrand factor , tissue type plasminogen activator , and plasminogen activator inhibitor 1 levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic system parameters , plasminogen , tissue type plasminogen activator ( t PA ) and urokinase PA , PA inhibitor type 1 ( PAI 1 ) and PAI type 2 concentrations and PAI 1 activity , were quantified in plasma and pleural fluid . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , and as such is thought to play an important role in the regulation of extracellular matrix remodeling . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , and as such is thought to play an important role in the regulation of extracellular matrix remodeling . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma samples were analyzed for the concentration of C reactive protein ( CRP ) , fibrinogen , d dimer , plasminogen activator inhibitor type 1 ( PAI 1 ) antigen and activity , tissue type plasminogen activator ( t PA ) antigen and activity , factor 7 ( FVII ) antigen , FVII coagulant activity ( FVII : C ) and activated FVII ( FVIIa ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) is the primary inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , paraffin embedded material from 84 human pituitary adenomas ( acromegaly n=18 , Cushing ' s disease n=21 , prolactinoma n=18 , thyroid stimulating hormone secreting adenoma n=1 , nonsecreting adenoma n=26 ) and 9 nontumourous anterior pituitary lobes ( obtained from patients with prostate cancer ) was immunohistochemically analysed for expression of MMP 2 , MMP 9 , tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We determined the plasma prothrombin time ( PT ) , activated partial thromplastin time ( APTT ) , activity of tissue type plasminogen activator ( t PA ) , activity of plasminogen activator inhibitor 1 ( PAI 1 ) and fibrinogen ( Fg ) level of 24 endogenous hyper triglyceridemic patients and 34 healthy controls , and observed their relationship to serum lipids and apolipoproteins . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined effects of pitavastatin ( NK 104 ) , a relatively novel and long acting statin , on expression of tissue factor ( TF ) in human monocytes ( U 937 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue type plasminogen activator ( t PA ) in human aortic smooth muscle cells ( SMC ) and human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we investigated the impact of HA on the synthesis of tissue type plasminogen activator ( t PA ) and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) in cultured human peritoneal mesothelial cells ( MC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the prolonged effect of HRT on the fibrinolytic system and to determine whether two common polymorphisms in the plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) genes modulate this effect . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue specimens from eight patients with failing or failed infra inguinal vein bypasses and three specimens from normal veins were harvested to study urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) by in situ hybridization and immunohistochemistry . ^^^ Urokinase , tissue type plasminogen activator and plasminogen activator inhibitor 1 expression in severely stenosed and occluded vein grafts with thrombosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Incorporation of the fluorescently silent 4 fluorotryptophan analog into PAI 1 permitted us to observe changes in the intrinsic tryptophan fluorescence of two chain tissue type plasminogen activator ( tPA ) and the proteinase domain of tPA during the inhibition reaction . ^^^ Distortion of the catalytic domain of tissue type plasminogen activator by plasminogen activator inhibitor 1 coincides with the formation of stable serpin proteinase complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Until the age of 6 months , plasma concentrations of plasminogen and alpha ( 2 ) antiplasmin are decreased to 50 % and 80 % of adult values , respectively , while plasma concentrations of tissue type plasminogen activator are decreased and plasminogen activator inhibitor 1 are increased throughout childhood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Twenty PVR , PDR or pucker membranes were examined to identify the cells with cell specific markers and to detect the expression of urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) or plasminogen activator inhibitor 1 ( PAI 1 ) by in situ hybridization and by immunohistochemistry . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The binding of plasminogen activator inhibitor 1 ( PAI 1 ) to serine proteinases , such as tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , is mediated by the exosite interactions between the surface exposed variable region 1 , or 37 loop , of the proteinase and the distal reactive center loop ( RCL ) of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Specifically , we consider polymorphisms in coagulation factors ( fibrinogen , prothrombin , FV Leiden , FVII , and FXIII ) ; fibrinolytic factors ( tissue type plasminogen activator , plasminogen activator inhibitor 1 , and thrombin activatable fibrinolysis inhibitor ) ; platelet surface receptors ; methylenetetrahydrofolate reductase ; endothelial NO synthase ; and the antioxidant enzymes paraoxonase and plasma glutathione peroxidase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the patients and in 25 control subjects , plasma levels of tissue type plasminogen activator , plasminogen activator inhibitor ( PAI 1 ) , D dimer , prothrombin fragment 1 + 2 ( F 1 + 2 ) , total cholesterol , triglycerides and fibrinogen had been measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the oE ( 2 ) group , significant percentage changes from baseline versus placebo in cycle 13 were found in fibrinogen , 5 . 4 % ( P < . 05 ) ; factor 7 , 7 . 3 % ( P < . 05 ) ; thrombin antithrombin 3 complexes , 13 . 3 % ( P < . 05 ) ; tissue type plasminogen activator ( t PA ) , 17 . 3 % ( P < . 001 ) ; and PAI 1 , 54 . 3 % ( P < . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We measured the effects of cardiopulmonary bypass ( CPB ) on PAI 1 antigen and tissue type plasminogen activator ( tPA ) antigen and activity in 31 patients taking an ACE inhibitor ( ACEI ) who were randomized to continue ACEI until the morning of surgery ( ACEI group , n=19 ) or to discontinue it 48 hours before surgery ( No ACEI group , n=12 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( T PA ) and plasminogen activator inhibitor ( PAI 1 ) in human follicular fluid during gonadotropin induced ovulation ] . ^^^ Concentrations of tissue type plasminogen activator in FF of patients with 5 or more mature oocyte were significantly lower ( 0 . 017 vs 0 . 056 ng / mg ) and levels of PAI 1 higher ( 3 . 49 vs 2 . 9 ng / mg ) in comparison to cases involving < 5 oocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the study was to evaluate associations between some fibrinolytic factors : antigens of tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , and thrombin activatable fibrinolysis inhibitor ( TAFI ) , and IMT in a population of renal transplant recipients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We analyzed the effects of MM LDL on brain capillary endothelial expression of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) , and thrombomodulin ( TM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The antithrombotic mechanism of novel compounds is not relevant to hemostatic systems or functions of platelet aggregation directly , but they can promote endothelial cells to release tissue type plasminogen activator ( t PA ) and inhibit the activity of plasminogen activator inhibitor 1 ( PAI 1 ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activity of type 1 plasminogen activator inhibitor ( PAI 1 ) in rat plasma or platelet released substances and tissue type plasminogen activator ( tPA ) in rat plasma was assayed by use of a chromogenic substrate . ^^^ PO : persistent occlusion CR : cyclic reflow PP : persistent patency PAI 1 : type 1 plasminogen activator inhibitor tPA : tissue type plasminogen activator PBS : phosphate buffer solution IC ( 50 ) : 50 % of inhibitory concentration PRP : platelet rich plasma ADP : adenosine diphosphate AA : arachidonic acid PAF : platelet activating factor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured markers of nutrition [ normalized protein catabolic rate ( nPCR ) , albumin , prealbumin , insulin like growth factor 1 ( IGF 1 ) , transferrin , and cholesterol ] , markers of endothelial damage and function [ tissue type plasminogen activator ( tPA ) , thrombomodulin ( TM ) , von Willebrand factor ( vWF ) , and NO 3 ( representing NO ) ] , markers of a coagulable state [ fibrinogen and plasminogen activator inhibitor 1 ( PAI 1 ) ] , markers of inflammation [ tumor necrosis factor alpha ( TNF alpha ) and C reactive protein ( CRP ) ] , and other endothelial injury factors [ lipoprotein ( a ) [ Lp ( a ) ] and homocysteine ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 is the principal inhibitor of tissue type plasminogen activator ( t PA ) , trapping the proteinase as an acyl enzyme covalent complex ( approximately 105 kDa ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHOD : We studied urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) , urokinase receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) expression by in situ hybridization and by immunohistochemistry in 14 NF 2 and 15 sporadic patients with 34 schwannomas . uPAR and vitronectin immunohistochemistry were also studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : In a case control study , we studied polymorphisms of the genes coding for factor 2 ( G20210A ) , factor 5 ( G1691A ) , methylenetetrahydrofolate reductase ( C677T ) , tissue type plasminogen activator ( tPA ; insertion / deletion ) , plasminogen activator inhibitor 1 ( PAI 1 ; 4G / 5G ) , and vascular endothelial growth factor ( VEGF ; C936T ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the effect of repeated thoracenteses on the fluid characteristics and the levels of various cytokines , including tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , IL 5 , IL 6 , and IL 8 , and of plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator in malignant pleural effusion and its clinical significance . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To clarify the role of proteins involved in the regulation of fibrinolysis during corneal angiogenesis , we have studied corneal vessel formation in mice deficient for urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) and thrombin activatable fibrinolysis inhibitor ( TAFI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study of nasal mucosa , we investigated the presence of mRNA of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and plasminogen activator inhibitor 2 ( PAI 2 ) using reverse transcription polymerase chain reaction ( RT PCR ) and in situ hybridization , and compared these results with their localization in immunostained tissues . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , there was no significant difference of plasminogen activator inhibitor 1 ( PAI 1 ) , pro uPA , and tissue type plasminogen activator ( tPA ) concentrations in the medium between dDAVP treatment and control . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrinopeptide A ( FPA ) , fibrin d dimer , thrombin antithrombin ( TAT ) complex , prothrombin fragment 1 . 2 ( F1 . 2 ) , urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured before and after therapy , as was the cellular expression of the genes for tissue factor ( TF ) and interleukin 1 beta ( IL 1 beta ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activity of the newly formed PAI 1 was investigated by incubating platelets in the presence of tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Quantitative gene expression analysis ( real time RT PCR ) was performed for tissue factor ( TF ) , TF pathway inhibitor ( TFPI ) , tissue type plasminogen activator ( t PA ) , urokinase type PA ( u PA ) , PA inhibitor 1 ( PAI 1 ) , and PAI 2 in peripheral white blood cells ( PBC ) as well as in alveolar macrophages ( AM ) , type 2 pneumocytes ( ATII ) , endothelial cells ( EC ) and smooth muscle cells ( SMC ) , all obtained by laser microdissection . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In a prospective , longitudinal , case control study , we studied plasma plasminogen activator inhibitor type 1 ( PAI 1 ) activity , tissue type plasminogen activator antigen ( t PA : Ag ) , d dimer , prothrombin fragment 1+2 ( F 1+2 ) , and thrombin antithrombin 3 complex ( TAT ) levels in 55 consecutive patients on admission , 1 week , 1 month , and 3 months after an ischemic stroke . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All patients underwent a screening prothrombin time , activated partial thromboplastin time , fibrinogen ( Fg ) , coagulation factors of the intrinsic and extrinsic pathways , antithrombin 3 ( AT 3 ) , protein C ( PC ) , protein S ( PS ) , resistance to activated protein C , prothrombin activation fragment 1+2 ( F1+2 ) , plasminogen , tissue type plasminogen activator ( t PA ) , plasminogen tissue activator inhibitor type 1 ( PAI 1 ) , anticardiolipin antibodies type M and G ( ACA IgM and ACA IgG ) , lupus anticoagulant antibodies , and antiprothrombin antibodies type M and G ( aPT IgM and aPT IgG ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Semi quantitative analysis was used to observe stained intensity and area of tissue type plasminogen activator , urokinas type plasminogen activator / plasminogen activator inhibitor ( tPA , uPA / PAI 1 ) in remnant renal tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Binding of vitronectin to NBD P 9 PAI 1 does not affect SI but results in a 2 . 0 6 . 5 fold decrease in the limiting rate constant ( klim ) of RCL insertion for urokinase type plasminogen activator at pH 6 . 2 8 . 0 and for tissue type plasminogen activator at pH 6 . 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen activation system was also altered in tumors with a decrease of urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) and a strong increase of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma was collected for ELISA analysis . mRNA for MMP 9 as well as plasminogen activator inhibitor 1 ( PAI 1 ) was increased in unstable plaques , while tissue type plasminogen activator ( tPA ) expression was similar in stable and unstable plaques . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of thrombin antithrombin 3 , fibrin D dimer , von Willebrand factor , tissue type plasminogen activator , and plasminogen activator inhibitor 1 were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Epithelial tissue type plasminogen activator expression , unlike that of urokinase , its receptor , and plasminogen activator inhibitor 1 , is increased in chronic venous ulcers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of the inflammatory marker C reactive protein ( CRP ) were determined at basal conditions , and the fibrinolytic parameters tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were measured at basal conditions and after a standardized venous occlusion ( VO ) of the forearm . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Genes such as gelatinase A ( MMP 2 ) , cathepsin L , tissue inhibitor metalloproteinases 2 ( TIMP 2 ) and 3 ( TIMP 3 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) , urokinase plasminogen activator ( tPA ) , endothelin 1 , calponin , carboxypeptidase D and calponin acidic were down regulated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether the increased concentration in blood of total tissue type plasminogen activator ( t PA ) , accompanying increased plasminogen activator inhibitor type 1 ( PAI 1 ) seen with diabetes reflects increased free t PA and hence activity or simply increased t PA complexed with PAI 1 , we measured each in 39 people with type 2 diabetes . ^^^ BACKGROUND : Type 2 diabetes is known to be associated with increased concentrations in blood of total tissue type plasminogen activator ( t PA ) [ free t PA plus t PA complexed with plasminogen activator inhibitor type 1 ( PAI 1 ) ] and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In a chromogenic substrate based urokinse ( uPA ) / PAI 1 assay and a tissue type plasminogen activator ( tPA ) mediated clot lysis assay , ZK 4044 inhibited human PAI 1 activity with IC 50 values of 644+ / 255 and 100+ / 90 nM , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The content of plasma nitric oxide ( NO ) , and activities of tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor ( PAI ) were detected after the hormone was injected for 4 and 8 weeks and after the drugs were given for 4 weeks . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NSO produced a concentration dependent inhibition of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) , fibrinogen ( Fbg ) and D dimer ( DD ) were investigated after those rabbits had been treated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RT PCR assay for urokinase type plasminogen activator ( uPA ) , its cellular receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tumor necrosis factor ( TNF ) alpha was performed to assess the cecal tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Tissue type plasminogen activator deficient ( tPA ( / ) ) , urokinase type plasminogen activator deficient ( uPA ( / ) ) , plasminogen activator inhibitor 1 deficient ( PAI 1 ( / ) ) , alpha 2 antiplasmin deficient ( alpha 2 AP ( / ) ) mice , and their wild type counterparts were used . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured levels of von Willebrand factor antigen , thrombomodulin , tissue type plasminogen activator , plasminogen activator inhibitor 1 and d dimer in the plasma using enzyme linked immunosorbent assay . ^^^ RESULTS : We found increased levels of von Willebrand factor antigen ( p = 0 . 01 ) , tissue type plasminogen activator ( p = 0 . 01 ) , and decreased levels of thrombomodulin ( p = 0 . 008 ) in the patients when compared to controls , while levels of plasminogen activator inhibitor 1 were not different . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , IL 6 , IL 8 , macrophage inflammatory protein ( MIP ) 1beta , monocyte chemoattractant protein ( MCP ) 1 , plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) were measured using enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) protein in atrium was determined by Western Blot analysis and immunohistochemical staining . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study attempted to verify the existence of a relationship between SOX documented by Cu / Zn superoxide dismutase ( Cu / Zn SOD ) and fibrinolysis analyzed by tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasmin / antiplasmin ( PAP ) complexes in dialysis patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Skeletal muscle regeneration induced by injury has been analyzed in urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen ( Plg ) and plasminogen activator inhibitor 1 ( PAI 1 ) deficient mice and has demonstrated profound effects of these molecules on the fibrotic state and the inflammatory response , which contribute to muscle repair . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of tissue type plasminogen activator ( tPA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Messenger RNA levels of tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 were analysed using Taqman real time reverse transcriptase polymerase chain reaction and protein release by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Only spironolactone significantly decreased the molar ratio of PAI 1 to tissue type plasminogen activator ( t PA ) in hypertensive subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using mice deficient in tissue type plasminogen activator ( tPA / ) and urokinase plasminogen activator receptor ( uPAR / ) , we investigated the involvement of the PA system on the clinical and pathological features of experimental allergic encephalomyelitis , an animal model of multiple sclerosis . tPA / mice suffered an early and a more severe acute disease characterized by incomplete recovery when compared to wild type controls , with significantly higher CNS levels of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study analyzed the effect of increasing the atorvastatin dose to 80 mg / day on indices of inflammation ( C reactive protein or CRP ) , thrombogenesis ( prothrombin fragment [ F1+2 ] ) and fibrinolysis ( tissue type plasminogen activator antigen , t PA , and its inhibitor PAI 1 ) in high risk patients with ischemic heart disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were collected before surgery for determination of prothrombin fragments F 1 and 2 , thrombin antithrombin complex , tissue type plasminogen activator ( tPA ) activity and antigen , plasminogen activator inhibitor 1 ( PAI 1 ) activity and antigen , D dimer , homocysteine , fibrinogen , in plasma , and hematocrit in blood , and the patients underwent transcranial Doppler ultrasonology for evaluation of microembolic signals ( MES ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured plasma high sensitivity C reactive protein ( hsCRP ) , interleukin 6 ( IL 6 ) , leptin , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and tumour necrosis factor alpha ( TNF alpha ) after each of the two treatment periods and in 13 age and sex matched lean individuals . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate plasminogen activator activity ( PAA ) , tissue type plasminogen activator ( t PA ) antigen level and plasminogen activator inhibitor 1 ( PAI 1 ) antigen in normal canine gingival tissue samples , gingivitis as well as in different stages of periodontal disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
STUDY OBJECTIVES : To measure tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , and transforming growth factor ( TGF ) beta 1 in loculated and free flowing pleural effusions caused by malignancy , tuberculosis ( TB ) , and pneumonia and their relationship with plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( tPA ) and to compare the differences between loculated and free flowing effusions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiologic inhibitor of tissue type plasminogen activator in plasma , and is elevated in a variety of clinical situations that are associated with increased risk of ischemic cardiovascular events . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiologic inhibitor of tissue type plasminogen activator in plasma , and is elevated in a variety of clinical situations that are associated with increased risk of ischemic cardiovascular events . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Interestingly , we observed that fibrin also induced the expression of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 by casein zymographic and reverse zymographic analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Markers of fibrinolysis , thrombin activatable fibrinolysis inhibitor ( TAFI ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) levels were studied for the evaluation of short term effects of raloxifene administration in postmenopausal women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND AND PURPOSE : The tissue type plasminogen activator ( tPA ) 7351C > T and the plasminogen activator inhibitor type 1 ( PAI 1 ) 675 4G > 5G polymorphisms influence transcriptional activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OE DEP and OE UFP exposure reduced plasminogen activator inhibitor 1 ( PAI 1 ) production by the cells but did not affect the production of thrombomodulin , tissue type plasminogen activator , or urokinase type plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Forty eight Sprague Dawley ( SD ) rats were randomly divided into the model group , sham operation group , and control group for indexes observation of triphenyltetrazolium chloride ( TTC ) dyeing , neurological deficit , plasma tissue type plasminogen activator ( tPA ) activity , plasminogen activator inhibitor ( PAI ) activity , thromboxane B ( 2 ) ( TXB ( 2 ) ) content , and 6 keto prostaglandin ( 6 keto PGF ( 1alpha ) ) content . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The most upregulated gene identified encodes plasminogen activator inhibitor 1 ( PAI 1 , Serpine 1 ) , a protease inhibitor that blocks urokinase plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following parameters were determined : prothrombin time ( PT ) , activated partial thromboplastin time ( aPTT ) , factors 7 , 10 , and 12 activity , fibrinogen levels , thrombin antithrombin complex levels , protein C and S antigen , antithrombin activity , plasminogen activator inhibitor type 1 ( PAI 1 ) antigen , tissue type plasminogen activator ( t PA ) antigen , plasminogen activity and fibrin degradation products ( FbDP ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Restoration of collagen degradation by GSH is associated with a reduction of type 1 plasminogen activator inhibitor ( PAI ) 1 expression / activity as well as recovery of the activities of cell / extracellular matrix associated tissue type plasminogen activator and plasmin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have studied tissue type plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) , and d dimer levels in BS patients with and without thrombosis both in the acute and chronic phases along with suitable diseased and healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This turnover is regulated by matrix metalloproteinases ( MMPs ) , tissue inhibitor of matrix metalloproteinases ( TIMPs ) , and the plasminogen activation system , including tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured AGE peptides , markers of endothelial dysfunction ( von Willebrand factor , soluble E selectin , plasminogen activator inhibitor 1 , tissue type plasminogen activator , soluble vascular cell adhesion molecule 1 ) and markers of inflammatory activity ( soluble intercellular adhesion molecule 1 , C reactive protein , secretory phospholipase A 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The paper is dedicated to the study of structure and physiological functions of tissue type plasminogen activator and its inhibitor PAI 1 , changes of these parametres in normal and pathology conditions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At baseline , on days 1 , 3 , 7 and 14 ( 7 days after thienopyridines discontinuation ) we measured ADP induced and spontaneous platelet aggregation ( PA ) , levels of prothrombin fragment 1+2 ( F1+2 ) , thrombin antithrombin complex ( TAT ) , von Willebrand factor ( vWF ) , fibrinogen , tissue type plasminogen activator antigen ( tPA ) , plasminogen activator inhibitor activity ( PAI ) and D dimer ( Dd ) , and counted platelet number . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular Endothelial Growth Factor ( VEGF ) , tissue type Plasminogen Activator ( tPA ) , Plasminogen Activator Inhibitor 1 ( PAI 1 ) and lipoprotein ( lp ) ( a ) influence the pro coagulant status . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , the expressions of molecules involved in MMP activating and inhibitory pathways ( urokinase type plasminogen activator and tissue type plasminogen activator ; TIMP 1 , TIMP 2 , and plasminogen activator inhibitor 1 ) were found to be increased after abdominal irradiation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Neither L arginine nor ramipril alone affected basal plasminogen activator inhibitor 1 or tissue type plasminogen activator ( t PA ) antigen in these salt replete subjects in whom plasma renin activity was suppressed ( mean+ / SD 0 . 7+ / 0 . 5 ng angiotensin I / mL per hour ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) regulates vascular contractility through the low density lipoprotein related receptor ( LRP ) , and this effect is inhibited by plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Bone matrix turnover is regulated by matrix metalloproteinases ( MMPs ) , tissue inhibitors of matrix metalloproteinases ( TIMPs ) , and the plasminogen activation system , including tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These differences include decreased plasma concentrations of plasminogen , tissue type plasminogen activator and alpha 2 antiplasmin , increased plasma concentrations of plasminogen activator inhibitor 1 , as well as a decrease in both plasmin generation and overall fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied levels of major plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) in lungs of preterm infants with RDS . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A cross sectional study was therefore performed in 107 type 2 diabetic patients recruited from the Denver Metropolitan Area to examine the effect of impaired fibrinolysis , as assessed by the ratio of plasminogen activator inhibitor ( PAI 1 ) to tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The central components of the PA system are the proteolytic activators , urokinase plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) , plasminogen ( plg ) and its degradation product , plasmin , together with the major inhibitors of this system , plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Citrated plasma blood samples were taken before and 30 minutes after a maximal exercise treadmill test , and levels of tissue type plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI 1 ) antigen and lipoprotein a , Lp ( a ) , [ all ELISA ] were measured as indices of fibrinolytic function . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Clinical as well as experimental evidence suggests that vascular overexpression of plasminogen activator inhibitor ( PAI ) 1 , the primary physiological inhibitor of both urokinase and tissue type plasminogen activator , may be involved in the pathophysiology of atherosclerosis and cardiovascular disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma von Willebrand factor antigen ( vWF : Ag ) , thrombomodulin , tissue type plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) , and D dimer were determined by immunoenzymatic assay at baseline , and at 6 , 12 , and 18 months . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) , fibrinogen ( Fbg ) and D dimer ( DD ) were investigated after those rabbits had been treated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The functions of the serpin PAI 1 ( plasminogen activator inhibitor 1 ) are based on molecular interactions with its target proteases uPA and tPA ( urokinase type and tissue type plasminogen activator respectively ) , with vitronectin and with endocytosis receptors of the low density lipoprotein family . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : We investigated whether the ratio of oleic to palmitic acid [ and that of monounsaturated to saturated fatty acids ( MUFA : SFA ) ] in the diet affects postprandial concentrations of triacylglycerols , tissue factor ( TF ) , fibrinogen , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression levels of tissue type plasminogen activator ( t PA ) , bone carboxyglutamate protein ( BGP ) , c sis , and an unknown protein were downregulated in the sera of patients with IONFH , whereas the other 3 proteins , including plasminogen activator inhibitor type 1 ( PAI 1 ) , crosslaps , and anti p 53 antibody , were upregulated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of cyclic strain on the production of tissue plasminogen activator ( tPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) by cultured endothelial cells ( EC ) were examined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In cell culture , tPA was released only from stromal cells and uPA only from glandular cells as determined by SDS PAGE followed by fibrin overlay technique , but PA inhibitor type 1 ( PAI 1 ) was secreted by both stromal and glandular cells . ^^^ The effect of the peptide hormones , hCG , GnRH , PRL , as well as cAMP in cell culture on the secretion of PAs and PAI was similar to that of estradiol , while forskolin demonstrated definitely more stimulative effect on tPA than uPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 levels were dose dependently enhanced by dexamethasone after a pretreatment period of 5 days while simultaneously a decreased tPA level was observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to determine the mechanism by which parathyroid hormone ( PTH ) stimulates plasminogen activator ( PA ) activity in rat osteoblasts , we investigated the effect of human PTH ( 1 34 ) [ hPTH ( 1 34 ) ] on the synthesis of mRNAs for tissue type PA ( tPA ) , urokinase type PA ( uPA ) , and PA inhibitor 1 ( PAI 1 ) , and on release of PA activity and PAI 1 protein in both normal rat calvarial osteoblasts and UMR 106 01 osteogenic sarcoma cells . hPTH ( 1 34 ) ( 0 . 25 25 nM ) decreased PAI 1 mRNA and protein , and increased PA activity in both cell types in a dose dependent manner with ED 50 of about 1 nM for both responses . ^^^ Forskolin and isobutylmethylxanthine also stimulated PA activity and decreased PAI 1 protein and mRNA in both cell types . hPTH ( 1 34 ) did not show any consistent effect on tPA and uPA mRNA in calvarial osteoblasts , but a modest ( two fold ) increase of both mRNAs was observed in UMR 106 01 cells treated with 25 nM hPTH ( 1 34 ) . ^^^ The reduction of PAI 1 protein by PTH results in enhanced action of both tPA and uPA , and would contribute to the specific roles of these PAs in bone . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Colonies inducing lysis ( clone C+ and H+ ) or no lysis ( clones B and M ) were isolated and tested for mRNA levels of uPA , tPA , uPA receptor ( uPAR ) and the 3 PA inhibitors ( PAI ) , PAI 1 , PAI 2 and protease nexin 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , immunocytochemical analysis showed that PAI 1 antigen is present almost exclusively in the cytoplasm but was not observed on the surface of the cells , whereas tPA antigen is abundant on the plasma membrane of tPA treated cells as well as intracellularly . ^^^ Competition studies using unlabelled compounds showed that native tPA and tPA B chain ( the proteinase domain ) , as well as the inactive derivatives , B chain inactivated with D Phe Pro Arg chloromethane and tPA PAI 1 complex , caused a considerable quenching of the binding of 125I tPA to HUVEC , whereas the isolated A chain had no demonstrable effect . ^^^ Two components ( apparent molecular masses 38 kDa and 56 kDa ) reacting with tPA but lacking PAI 1 antigen determinants were identified . ^^^ One is mediated by PAI 1 , which binds and inactivates tPA with a functional active site . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the major physiologic inhibitor of tissue plasminogen activator ( tPA ) , plays a crucial role in the regulation of fibrinolysis . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the major physiologic inhibitor of tissue plasminogen activator ( tPA ) , plays a crucial role in the regulation of fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , no changes in tissue PA ( tPA ) and PAI 1 cell surface antigen expression were evident under analogous conditions over a 24 hr period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the principal physiological inhibitor of tissue plasminogen activator ( tPA ) , is a protein of 379 amino acids and belongs to the SERPIN family of serine protease inhibitors . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the principal physiological inhibitor of tissue plasminogen activator ( tPA ) , is a protein of 379 amino acids and belongs to the SERPIN family of serine protease inhibitors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , in contrast to tPA , such treatment led to a 3 fold increase in plasminogen activator inhibitor ( PAI ) activity , whereas the amount of PAI type 1 antigen was unchanged . ^^^ Microplasmin , which has no kringle domains , was less effective in reducing tPA antigen biosynthesis or enhancing PAI activity in HUVEC . ^^^ Kringle domains of plasmin affected neither tPA antigen nor PAI activity of the cells . ^^^ Other proteases including chymotrypsin , trypsin , and collagenase at comparable concentrations did not have a significant effect on the biosynthesis of tPA antigen or PAI activity of HUVEC . ^^^ Thrombin stimulated the biosynthesis of tPA and PAI 1 antigens by HUVEC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HPMC PAI 1 is increased by transforming growth factor beta ( TGF beta ) and tumor necrosis factor alpha ( TNF alpha ) , as is tPA release , while PAI 1 mRNA is unchanged and tPA mRNA is increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a rapid inhibitor of tissue plasminogen ( tPA ) in vivo . ^^^ Evidence suggests that the level of plasma PAI 1 activity is responsible for the regulation of the whole fibrinolytic process through this tPA / PAI 1 interaction . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a rapid inhibitor of tissue plasminogen ( tPA ) in vivo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) levels increased from 59 + / 35 micrograms / l to 113 + / 39 micrograms / l at 6 h ( P < 0 . 01 ) . tPA PAI 1 complexes increased from 0 . 15 + / 0 . 07 to 0 . 69 + / 0 . 21 nmol / l at 6 h ( P < 0 . 01 ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) levels increased from 59 + / 35 micrograms / l to 113 + / 39 micrograms / l at 6 h ( P < 0 . 01 ) . tPA PAI 1 complexes increased from 0 . 15 + / 0 . 07 to 0 . 69 + / 0 . 21 nmol / l at 6 h ( P < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were collected at 1 , 2 , 4 , 6 , 8 and 12 hours after administration to assay prothrombin activity , partially activated thromboplastin time , and the activation to tPA and PAI 1 . ^^^ A significant increase ( P < 0 . 001 ) of tPA activity and a reduced inhibition of fibrinolytic systems by PAI 1 were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present prospective follow up study the effect of 16 weeks insulin treatment and glycemic regulation on plasma levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , the main regulators of fibrinolysis , was investigated in 19 type 2 diabetic patients with secondary failure to sulphonylureas . ^^^ Compared to 27 healthy subjects levels of tPA and PAI 1 were not significantly increased in type 2 diabetic patients before metabolic intervention . ^^^ Although a hypofibrinolytic state due to an increase of PAI 1 levels was previously reported in obese hyperinsulinemic patients , no effect of insulin treatment on both tPA and PAI 1 levels was observed in the present study including patients with only slightly increased body mass index ( median 26 . 0 kg / m2 ) . ^^^ In healthy subjects tPA and PAI 1 was correlated to serum insulin ( R = 0 . 54 , R = 0 . 56 ) and triglycerides ( R = 0 . 46 , R = 0 . 40 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The former group was examined 14 to 20 years after surgery , and was found to have lower mean plasminogen activator inhibitor type 1 ( PAI 1 ) activity ( 8 . 4 5 32 U / mL , P < . 001 ) , tissue plasminogen activator ( tPA ) antigen concentrations ( 7 . 2 5 12 micrograms / L , P < . 01 ) , body mass index ( BMI ) , and fasting plasma insulin , triglyceride , and cholesterol levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we tried to elucidate the mechanism of coagulation fibrinolysis with relation to kks by measuring of Thrombin / Antithrombin 3 complex ( TAT ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI ) complex ( tPA . ^^^ C and active PAI significantly increased the first trimester ( TAT 4 . 31 + / 2 . 05 ng / ml , tPA . ^^^ C 39 . 52 + / 17 . 34 ng / ml , active PAI 39 . 58 + / 15 . 29 ng / ml , n = 20 M + / SD P < 0 . 001 ) to the third trimester ( TAT 6 . 39 + / 1 . 93 ng / ml , tPA . ^^^ C 57 . 94 + / 30 . 80 ng / ml , active PAI 304 . 24 + / 148 . 64 ng / ml , n = 20 M + / SD P < 0 . 001 ) as compared with those of non pregnant women ( TAT 1 . 60 + / 0 . 89 ng / mg , tPA . ^^^ C 93 . 38 + / 56 . 05 ng / ml , PIC 1 . 03 + / 0 . 94 micrograms / ml n = 20 M + / SD P < 0 . 02 ) in UV , but active PAI markedly decreased ( active PAI 244 . 18 + / 87 . 55 ng / ml n = 20 M + / SD P < 0 . 02 ) as compared with those in PV ( TAT 6 . 1 + / 2 . 09 ng / ml , tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This systemic information was evaluated in 20 cases of normal gravidas from 28 to 42 weeks of gestation as a control by measuring plasma Thrombin / Antithrombin 3 complex ( TAT ) , tissue plasminogen activator ( tPA ) plasminogen activator inhibitor ( PAI ) complex , active plasminogen inhibitor 1 ( active PAI ) , 2PI plasmin complex ( PIC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) activity , plasminogen activator inhibitor ( PAI ) activity , von Willebrand ' s factor ( vWF ) activity and antigen ( vWF : Ag ) , and factor 8 activity were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements of euglobulin clot lysis time ( ELT ) , fibrinogen , antithrombin 3 ( AT 3 ) , protein C ( PC ) , protein S ( PS ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) were done . ^^^ Results indicated that all patients had prolonged ELT , low tPA , elevated PAI , normal AT 3 , and PS . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We analyzed the fibrinolytic system in patients with chronic low back pain using a venous occlusion test to stimulate fibrinolysis , and we subsequently determined the levels of tissue plasminogen activator ( TPA ) and fast acting inhibitor of TPA ( PAI ) . ^^^ No clear explanation for this defective function of TPA in the patients was obtained , because no difference was seen in PAI level in basal samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of therapeutic and pharmacological concentrations of tiaprofenic acid , a non steroidal anti inflammatory drug ( NSAID ) , on the synthesis of the plasminogen activators , urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) , and the plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) , by human synovial membranes isolated from osteoarthritis ( OA ) and rheumatoid arthritis ( RA ) sufferers was evaluated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma levels of tissue plasminogen activator ( tPA ) antigen concentration and plasminogen activator inhibitor ( PAI ) activity were measured in a random sample of 260 subjects , 30 , 40 , 50 , or 60 years of age . ^^^ As compared to subjects lacking such signs , plasma tPA and PAI levels were significantly increased in the 60 year old group , and PAI tended to be increased in the 50 year old group . ^^^ This cross sectional population study provides independent data that patients with electrocardiographic signs of IHD have increased levels of both PAI and tPA antigen . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study is to evaluate the relationship between tissue plasminogen activator ( tPA ) / PA inhibitor ( PAI ) complex and PAI , and platelet in the circulating blood . tPA / PAI complex and active PAI ( act PAI ; Teijin Co . , Japan ) , and PAI antigen ( PAI ant ; Biopool AB , Sweden ) were assayed by enzyme immunoassay ( EIA ) . ^^^ The mean concentrations ( ng / 10 ( 9 ) platelets ) in supernatant from lysate of platelet rich plasma ( PRP ) and washed platelets were 27 . 1 + / 9 . 2 , 1 . 5 + / 1 . 2 in tPA / PAI complex , 188 . 7 + / 46 . 1 , 83 . 3 + / 7 . 5 in act PAI and 236 . 2 + / 41 . 6 , 191 . 9 + / 45 . 1 in PAI ant , respectively . ^^^ PRP was mixed with ADP ( 10 ( 5 ) , 10 ( 4 ) , 10 ( 3 ) M ) , for 3 min and the supernatant after centrifugation then was provided for assay of PAI and tPA / PAI complex . ^^^ Concentrations of act PAI , PAI ant and tPA / PAI complex dose dependently increased with ADP . ^^^ This study revealed that platelets contained quantities PAI ( as free PAI ) and released PAI during aggregation , and that a part of the PAI immediately formed a complex with tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to find out which hemostasis parameters would have the predictive value for the development of preeclampsia , modified antithrombin 3 ( ATM , representative of the antithrombin 3 serine esterase complex ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , beta thromboglobulin ( BTG ) , antithrombin 3 ( AT 3 ) , fibrinogen , fibrin ( ogen ) degradation product ( FDP ) , FDP D dimer and euglobulin lysis time ( ELT ) were measured in 20 normal non pregnant women , 21 normal pregnant women , 6 high risk pregnant women , 14 preeclampsia pregnant women , and 5 patients with disseminated intravascular coagulation ( DIC ) . ^^^ In the 2nd trimester , tPA , PAI , fibrinogen and FDP were significantly higher , and AT 3 was significantly lower in the preeclampsia than in the normal pregnant women , whereas in the 3rd trimester , tPA and AT 3 were significantly higher or lower , respectively , in the preeclampsia than in the normal pregnant women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Admission hemostatic markers that correlated with the severity of injury were : tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , D dimer ( D di ) , plasminogen ( Plg ) , proteins C and S ( PrC and PrS ) , antithrombin 3 ( ATIII ) , thrombin antithrombin complex ( TAT ) , kallikrein ( Kal : c ) , kinin ( Kin ) , C 1 esterase inhibitor ( C1Inh ) , and factor 7 clotting and antigen ( FVII : c , FVII : ag ) . ^^^ These data suggest that during the early course following burn injury , thrombogenicity is increased ( TAT increases ) because of a decrease in ATIII , PrC , and PrS ; and fibrinolysis activation ( D di increases ) occurs via an increase in tPA with a p value increase in PAI . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI ) activity and tissue plasminogen activator ( TPA ) antigen were measured in venous samples in 14 patients with unstable angina consisting of eight patients with organic stenosed coronary arteries and six patients with coronary spastic angina ( unstable angina group ) ; in 14 patients with stable exertional angina ( stable exertional angina group ) ; and in 14 patients with chest pain syndrome ( chest pain syndrome group ) . ^^^ There were no significant differences in PAI activity and TPA antigen levels between the stable exertional angina group and the chest pain syndrome group . ^^^ Furthermore , both PAI activity and TPA antigen levels in the unstable angina group decreased to the levels in the stable exertional angina group and the chest pain syndrome group after treatment ( p less than 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have examined the effect of cytoskeleton disrupting agents on the production and secretion of another proteolytic enzyme , tissue plasminogen activator ( tPA ) , and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , in human endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Good responders with a euglobulin clot lysis time less than or equal to 105 min showed significant release of tPA , PAI and vWF . ^^^ We have shown poor fibrinolytic response was related to either raised levels of PAI , poor release of both tPA and vWF , or poor release of tPA or vWF alone suggesting different mechanisms of fibrinolytic impairment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A plasminogen activator inhibitor ( PAI ) activity with molecular sizes of 54 and 58 kDa was also detected in PC 12 cell conditioned medium and formed high molecular mass complexes with released tPA . ^^^ Release of PAI activity was dependent on treatment with nerve growth factor ( NGF ) , whereas tPA synthesis and release were under control of a cyclic AMP dependent mechanism and increased on treatment with dibutyryl cyclic AMP [ ( But ) 2cAMP ] or cholera toxin . ^^^ Simultaneous treatment with NGF and ( But ) 2cAMP resulted in increases of both tPA and PAI release and enhancement of tPA PAI complex formation . ^^^ These results suggest that excess proteolytic activity may be detrimental to neuritic outgrowth and that not only PAI release but also tPA PAI complex formation is associated with production of large and stable neuritic outgrowth . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using immunofluorescence , immunoblotting , and immunoprecipitation experiments , evidence is presented that ( 1 ) the alloantigen Leka is not expressed in endothelial cells of an individual whose platelets are of the Leka / PIA1 phenotype , whereas the PIA 1 alloantigen is readily detectable in these cells , ( 2 ) that in contrast to HEL cells , which express platelet GP IIb / IIIa and are of the Leka / PIA1 phenotype , platelet GP IIb is immunologically undetectable in 12 O tetradecanoyl phorbol 13 acetate ( TPA ) treated K 562 cells despite the presence of platelet GP IIIa , and ( 3 ) that peripheral blood mononuclear cells do not express platelet GP IIb or GP IIIa on their cell surface . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This former value is 200 times higher than the reported rate constant for the interaction between PAI 2 and single chain tPA , suggesting that structures outside of the strained loop are responsible for the major differences in specificity between PAI 1 and PAI 2 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits the tissue plasminogen activator ( tPA ) and urokinase activation of plasminogen to plasmin , a protease of trypsin like specificity which is involved in a number of processes , including fibrinolysis , matrix degradation and angiogenesis . ^^^ Both phorbol esters and cAMP elevating compounds have been shown to modulate PAI 1 and tPA expression in endothelial cell culture . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits the tissue plasminogen activator ( tPA ) and urokinase activation of plasminogen to plasmin , a protease of trypsin like specificity which is involved in a number of processes , including fibrinolysis , matrix degradation and angiogenesis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following parameters were measured in the haemothorax liquid samples : clotting fibrinogen fraction ( Fg C ) , fibrin degradation D dimers , functional plasminogen , alpha 2 antiplasmin , alpha 2 macroglobulin , plasminogen tissue activator ( tPA Ag ) , type 1 tPA plasma inhibitor ( PAI ) , and haematocrit . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In human umbilical vein endothelial cells high glucose increased fibronectin , collagen 4 , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) mRNA levels 2 fold but did not affect type 4 and interstitial collagenase expression . ^^^ Acute treatment with phorbol esters resulted in increased collagen 4 , tPA , PAI 1 , and interstitial collagenase mRNAs ; the type 4 collagenase mRNA levels were instead suppressed to 50 % of control . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Another antibody , TPA 2 14 , partially inhibited the enzymatic activities of tPA towards the synthetic substrate and plasminogen , but it was not able to bind to the inactive tPA complexed with plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ The results indicate that tPA in these media exists either partially or almost entirely in a complex with PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Desmopressin ( 0 . 3 micrograms / kg BW i . v . ) , alone as well as in combination with dextran , increased 8 : C , vWF : Ag , protein C and tPA and decreased PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentrations of free plasminogen activator inhibitor ( PAI 1 ) and complex of tPA PAI 1 decreased from 8 : 30 to 16 : 30 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An impaired release of tPA and / or increased plasminogen activator inhibitor ( PAI ) is associated with thrombotic risk in the adult patient . ^^^ The purpose of this study was to assay the plasma levels of tPA , PAI , histidine rich glycoprotein ( HRG ) , and other fibrinolytic proteins in 15 severely stressed newborns . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To clarify the mechanism of this suppression , we investigated the effects of dexamethasone on PA inhibitor 1 ( PAI 1 ) , tissue type PA ( tPA ) , and urokinase type PA ( uPA ) expression and also on PAI 1 protein and PA activity in both normal rat calvarial osteoblasts and a clonal osteogenic sarcoma cell line , UMR 106 01 . ^^^ Although tPA and uPA protein could not be measured , these results suggest that glucocorticoids suppress PA activity predominantly by increasing PAI 1 synthesis in rat osteoblasts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 antigen , tPA antigen and thrombin antithrombin 3 complexes ( TAT ) levels were measured in 10 males with stable angina and type 2 diabetes mellitus and in 16 males with stable angina without diabetes or other risk factors ( hyperfibrinogenaemia , hyperlipidaemia , diabetes , hypertension , smoking and obesity ) known to increase PAI levels . ^^^ Because only diabetics with coronary artery disease ( CAD ) showed a decreased fibrinolytic capacity , a second study was performed on the 16 non diabetic CAD patients to determine whether submaximal workload induces significant changes of tPA and PAI levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelet aggregation and the plasma levels / activity of plasminogen activator ( tPA ) and its inhibitor ( PAI 1 ) were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) were measured in citrated plasma samples before and after venous occlusion before and at the end of the study period . tPA activity after venous occlusion and tPA capacity were significantly increased after doxazosin as compared to pretreatment values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Net regulation of tPA activity through the stimulatory actions of several calciotropic hormones and the promotion of PAI 1 formation by TGF beta could determine the amount of osteoblast derived TGF beta activated locally in bone . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Symptom limited treadmill exercise with thallium 201 myocardial single photon emission CT ( SPECT ) and fibrinolytic examinations ( tissue plasminogen activator antigen : tPA , plasminogen activator inhibitor 1 antigen : PAI 1 ) were performed 2 and 14 weeks after AMI in 13 patients with exercise training and in 12 patients without exercise training . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The endothelial cell synthesized haemostatic factors tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) and von Willebrand factor ( vWF ) were assayed in a cross sectional study of patients with immune thrombocytopenia ( ITP ) ( n = 12 ) and autoimmune haemolytic anaemia ( AIHA ) ( n = 3 ) , and compared with a simultaneously selected contrast group of other hospitalized patients with obscure blood cytopenia at the time of sampling . ^^^ In the autoimmune patient group , the three haemostatic variables were significantly correlated with orosomucoid levels , demonstrating the acute phase nature of the increased levels of vWF , tPA and PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PA inhibitor type 1 ( PAI 1 ) is an efficient inhibitor of tissue type PA ( tPA ) and urokinase type PA ( uPA ) that may therefore be instrumental for the control of plasminogen activation . ^^^ To investigate if coordinated regulation of PA and PA inhibitors take place in vivo in response to physiological signals , we have examined the regulation of PAI 1 and tPA in the ovary during gonadotropin induced ovulation . ^^^ We found that PAI 1 , as well as tPA activity and mRNA levels , were coordinately regulated by gonadotropins in a time dependent and cell specific manner , such that a surge of PA activity was obtained just prior to ovulation . ^^^ The coordinated regulation of tPA and PAI 1 in the ovary may fine tune the peak of PA activity which may be important for the regulation of the ovulatory process . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The work described here demonstrates the synthesis by human articular cartilage of plasminogen activator inhibitor 1 ( PAI 1 ) , a potent inhibitor of the serine protease tissue plasminogen activator ( tPA ) . ^^^ A scheme is presented indicating the probable importance of the serine proteases ( tPA and plasminogen ) and PAI 1 in cartilage degradation . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Disturbances in the tissue plasminogen activator / plasminogen activator inhibitor ( TPA / PAI ) system in systemic lupus erythematosus . ^^^ Despite the fact that the euglobulin lysis time in steady state was normal in all instances , disturbances in the tissue plasminogen activator / plasminogen activator inhibitor ( TPA / PAI ) system were found in all SLE patients : TPA activity was undetectable in all cases , whereas it was above 0 . 4 IU / ml in a control group . ^^^ In 72 percent of patients , the undetectable TPA activity was correlated with abnormally high PAI activity ; PAI levels were normal in all members of the control group , their mean value being 0 . 74 versus 8 . 63 IU / ml for SLE patients ( P less than . 01 ) . ^^^ Sixty eight percent of SLE patients had high levels of antiphospholipid antibodies , but no correlation with the disturbances of the TPA / PAI system was found . ^^^ It is concluded that most patients with SLE display severe abnormalities in the TPA / PAI anti thrombotic system and that these abnormalities may be related to the lupus thrombophilia , apparently multifactorial in its origin . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) , and tissue plasminogen activator ( tPA ) antigen and activity were measured before and after venous occlusion . ^^^ In the high risk group , tPA activity was significantly lower both before and after venous occlusion and PAI 1 levels were significantly higher . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A short term treatment of the undifferentiated Tera 2 cells with basic fibroblast growth factor ( bFGF ) increases uPA mRNA levels and the cell associated uPA activity , whereas the secretory tPA activity decreases . bFGF induces PAI 1 mRNA expression in the undifferentiated cells , but unlike PAI 1 protein after RA treatment , the inhibitor does not accumulate around the cells but is released in the medium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We present here some data on antigen increase in tissue Plasminogen Activator ( tPA ) and D 2 Dimer ( D2D ) detected during CPB and apparently not compensated by enhanced Plasminogen Activator Inhibitor type 1 ( PAI 1 ) activity . tPA concentration ( antigenic ) ranged around 6 . 15 ng / ml ( SD 5 . 6 ) before thoracotomy and 5 . 8 g / ml ( SD 4 . 74 ) 5 10 minutes after a heparin 250 IU / Kg bolus injection . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombotic events are often due to fibrinolytic defects such as impaired tissue plasminogen activator ( tPA ) synthesis and / or release or increased plasminogen activator inhibitor ( PAI ) levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To assess the postulated role of plasminogen activation in tumor invasion , we have investigated the cellular sites of synthesis for urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators and their inhibitors ( PAI 1 and PAI 2 ) in two human cutaneous neoplasia that differ in their metastatic potential . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following were measured on each sample : plasma factor 8 von Willebrand factor antigen ( FVIII vWF Ag ) , tissue plasminogen activator activity ( tPA ) , plasminogen activator inhibitor ( PAI ) , prostacyclin stimulating factor ( PGI 2 SF ) , fibrinopeptide A ( FPA ) , 11 dehydro thromboxane B 2 ( 11 dehydro TXB 2 ) and red cell deformability ( RCD ) . ^^^ The following parameters demonstrated significant circadian variation : tPA P less than 0 . 001 , PAI P less than 0 . 04 and 11 dehydro TXB 2 P less than 0 . 005 ( two way analysis of variance ) . tPA was highest at 16 . 00 20 . 00 and lowest at 08 . 00 ; PAI was highest at 08 . 00 and lowest at 20 . 00 ; TXB 2 had a peak at 16 . 00 and trough at 04 . 00 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
GM CSF and G CSF ( 1 ng / mL to 1 microgram / mL ) had no effect on the expression of either tissue plasminogen activator ( tPA ) or plasminogen activator inhibitor 1 ( PAI 1 ) by endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In all three types of cells , under control as well as under stimulated conditions , a high molecular weight form of PA was demonstrated by the gel overlay technique , most likely a complex of tPA with the PA inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human umbilical vein endothelial cells ( HUVEC ) were cultured in the presence of various glycosaminoglycans and the intracellular levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) measured by ELISA . 10 IU / ml heparin ( 90 micrograms / ml ) brought about a significant ( 20 fold ) increase in intracellular tPA levels over the 6 day culture period ; intracellular PAI 1 levels were significantly decreased ( by 60 70 % ) and culture growth rate promoted . ^^^ Chondroitin sulphate and heparan sulphate had no effect on tPA and PAI 1 levels but dermatan sulphate reduced PAI 1 significantly . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The structural events taking place during the reaction between PAI 1 ( plasminogen activator inhibitor 1 ) and the plasminogen activators sc tPA ( single chain tissue plasminogen activator ) and tc tPA ( two chain tissue plasminogen activator ) were studied . ^^^ Complexes were formed by mixing sc tPA or tc tPA with PAI 1 in slight excess ( on an activity basis ) . ^^^ Examination of the purified complexes by SDS / polyacrylamide gel electrophoresis ( SDS / PAGE ) and N terminal amino acid sequence analysis demonstrated that a stoichiometric 1 : 1 complex is formed between PAI 1 and both forms of tPA . ^^^ The proteolytic activity of sc tPA is demonstrated by its capacity to cleave the ' bait ' peptide bond in PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) antigen levels and plasminogen activator inhibitor ( PAI ) activity were measured in the plasma from 23 patients with familial amyloidotic polyneuropathy . ^^^ Both tPA levels and PAI activity were reduced to about half normal , even when the effects of patient age and body mass index were taken into account . ^^^ There were higher mean tPA levels and PAI activity in patients with mild disability than in those with moderate or severe disability , but these differences were not statistically significant . ^^^ This is the first reported disorder with reduced rather than increased basal tPA and PAI levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma samples of tissue plasminogen activator ( tPA ) antigen , and plasminogen activator inhibitor ( PAI 1 ) activity were obtained for the assay . ^^^ The tPA levels did not differ between the three groups , and there were no significant differences in other variables that covaried with PAI 1 levels , such as age , anthropometric variables , or serum lipid levels , which could confound the PAI 1 / food pattern relationship . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using a monoclonal antibody based assay , we measured the fibrin degradation product release in the supernatant of plasma clots obtained before and after venous occlusion ( VO ) in 30 patients with definite or suspected vascular thrombosis ( 19 definite and 2 suspected deep vein thrombosis , 6 recurrent superficial thrombophlebitis , 3 arterial occlusions of lower limbs ) . tPA and PAI 1 concentrations were determined using ELISA assays ; the post occlusion values were corrected for haemoconcentration . ^^^ These patients had respectively a deficient tPA release ( delta tPA = 1 ng / ml ) and an elevated PAI 1 level at rest ( 33 ng / ml ) . ^^^ This plasma clot test reflects the ability of free tPA to bind to fibrin ( the amount of which depends on the level of tPA and PAI 1 ) , and may be useful in the diagnosis of a hypofibrinolytic state . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relationships between tissue plasminogen activator ( tPA ) , its fast acting inhibitor ( PAI 1 ) and euglobulin clot lysis time ( ELT ) were investigated with healthy volunteers ' plasma . ^^^ Both tPA and PAI 1 showed the significant correlation with ELT . tPA had a significantly positive , not negative , correlation with ELT ( R = 0 . 387 , p less than 0 . 001 ) . ^^^ Higher correlation coefficients ( R = 0 . 580 , p less than 0 . 001 and R = 0 . 599 , p less than 0 . 001 ) were obtained between ELT and total PAI 1 or free PAI 1 than tPA or tPA PAI 1 complex ( R = 0 . 427 , p less than 0 . 001 ) . ^^^ The positive correlation was also obtained between tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Additionally , northern analysis of total mRNA levels at varying cell densities demonstrated modulation of tPA , PAI 1 , and TGF beta transcripts in normal urothelial cells as a function of growth in vitro , suggesting the presence of an intact regulatory pathway to control extracellular proteolysis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Normal plasma levels of tPA , urokinase and plasminogen activator inhibitor 1 were found by ELISA , and tPA antigen levels rose after desmopressin acetate infusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Preincubation of tPA with plasminogen activator inhibitor 1 ( PAI 1 ) was found to have little effect on binding , suggesting that tPA interacts at sites distinct from surface bound PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Aliquots were also obtained for measuring PAI activity and TPA levels . ^^^ The addition of exogenous TPA , activated protein C , or anti PAI antibodies increased the amount of clot lysis ; while the addition of anti TPA antibodies and EACA each prevented the post cuff increase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( tPA ) antigen , tPA activity and plasminogen activator inhibitor ( PAI ) activity were determined in 40 cases of acute myocardial infarction ( AMI ) at the onset and 2 4 weeks after the onset of the disease . 24 healthy subjects served as controls . ^^^ At 2 4 weeks , mean plasma levels of tPA and PAI activities of AMI patients had dropped to 1 . 5 + / 0 . 6 IU / ml and 9 . 5 + / 8 . 9 AU / ml , respectively , not significantly different from controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a natural plasminogen activator inhibitor , abolished tPA induced HUVEcell migration . ^^^ These results demonstrate for the first time that tPA is capable of stimulating endothelial cell migration in wound assays and this effect is susceptible to PAI 1 inhibition . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The correlation between the activity of tissue plasminogen activator ( TPA ) , levels of tissue plasminogen activator inhibitor ( PAI 1 ) antigen and serum lipids in healthy subjects ] . ^^^ In the present study , the correlation between the activity of tissue plasminogen activator ( TPA ) , the levels of tissue plasminogen activator inhibitor ( PAI 1 ) and serum lipids ( TC , TG , beta Lp , HDLC , LDLC and apolipoproteins ) were studied in 102 healthy subjects . ^^^ The activity of TPA showed negative correlation with the obesity rate ( r = 0 . 21 , p less than 0 . 05 ) but levels of PAI 1 antigen showed a positive correlation with the obesity rate ( r = 0 . 182 , p less than 0 . 1 ) . ^^^ The activity of TPA showed no correlation with serum lipids including apolipoproteins , while the levels of PAI 1 antigen showed a positive correlation between TG and beta Lp ( r = 0 . 292 , p less than 0 . 01 , r = 0 . 211 , p less than 0 . 05 ) . ^^^ These results indicate that TPA and PAI 1 play an important role in the development of thrombotic disease and atherosclerotic disease . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fluctuation of TPA and PAI 1 antigen levels in plasma : difference of their fluctuation patterns between male and female . ^^^ The daytime fluctuation of the fibrinolytic activity and the antigen levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in plasma were analyzed in normal male and female volunteers . ^^^ ECLT was always shorter in females than in males and parameter such as tPA and PAI 1 were also lower in females than in males . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fluctuation of tPA and PAI 1 antigen levels in plasma : difference of their fluctuation patterns between male and female . ^^^ The circadian fluctuation of the fibrinolytic activity and the antigen levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in plasma were analyzed in normal male and female volunteers . ^^^ ECLT was always shorter in females than in males and parameter such as tPA and PAI 1 were also lower in females than in males . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether the absence of tPA modulation by EGF reflected steady levels or the action of an anti activator , we assayed tPA , PAI 1 and tPA / PAI 1 complexes by zymography and immunological assays . ^^^ Under conditions in which EGF had no effect on tPA activity , tPA antigen paradoxically increased with a concomitant rise of tPA / PAI 1 complexes . ^^^ This indicated that tPA was rapidly inactivated through the formation of a complex , immunologically and electrophoretically related to tPA / PAI 1 . tPA antigen and tPA / PAI 1 complexes were modulated by EGF in a time and concentration dependent manner . ^^^ Evidence is presented here that two forms of PAI 1 are present in A 431 CM : an inactive form and an active form which neutralizes the tPA secreted , masking its enhancement by EGF in functional assays . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The regulation of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) synthesis was studied in cultured human mesothelial cells derived from omentum ( HOMC ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Factors affecting the stability of tPA and PAI 1 during storage and handling of human plasma for in vitro studies : implications in the determination of tPA and PAI 1 activities . ^^^ Factors that influence the physico chemical conditions of plasma ( e . g . pH , dilution , freezing , storage ) and thereby the stability of tPA and PAI 1 activities , have been studied and optimized using a solid phase fibrin tPA activity assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Modulation of tPA , PAI 1 and PAI 2 antigen and mRNA levels by EGF in the A 431 cell line . ^^^ To determine whether the absence of tPA modulation by EGF could be due to the action of inhibitors , we assayed tPA , PAI 1 , PAI 2 and tPA / PAI 1 complexes by immunological assays and zymography in A 431 serum free medium . ^^^ We found that , under conditions in which EGF had no effect on tPA activity , tPA antigen increased with a concomitant rise of tPA / PAI 1 complexes , indicating the action of an inhibitor . ^^^ Both tPA antigen and tPA / PAI 1 complexes were modulated by EGF in a time and concentration dependent manner . tPA / PAI 1 complex levels were lower than tPA levels , suggesting the presence of other inhibitors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TPA treated K 562 cells also synthesize and secrete platelet derived growth factor ( PDGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , urokinase plasminogen activator ( u PA ) and its specific inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These cells also produce and secrete the endothelial cell type PA inhibitor ( PAI 1 ) , which forms sodium dodecyl sulfate stable tPA / PAI 1 complexes in the culture medium . ^^^ Immunocytochemistry shows a predominant localization of uPA , tPA , and PAI 1 in neuronal cells , with only a very weak positivity detectable in the few glial cells present in these cultures . ^^^ The protein kinase C ( PKC ) activator 12 O tetradecanoylphorbol 13 acetate ( TPA ) stimulates the synthesis of both uPA and PAI 1 , resulting in a final increase in the plasmin generating capacity of neuronal cell cultures . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The 110 kDa protein co migrated with the PA PAI complexes and the 60 kDa protein co migrated with tissue Plasminogen Activator ( tPA ) . ^^^ TNF alpha increased 4 fold the amount of tPA PAI 1 complexes ( Mr 110 kDa ) detected in the conditioned media . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The thrombin induced PA 1 inhibited both tPA and urokinase , did not lose activity upon acidification , and was stable to sodium dodecyl sulfate and thiol reduction . ^^^ We conclude that physiologic concentrations of thrombin increase both PA 1 activity and tPA antigen in medium conditioned by human umbilical vein endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have examined the effect of phorbol esters and cAMP elevating compounds on tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) secretion . ^^^ These studies indicate that activation of a cAMP dependent pathway ( s ) in coordination with phorbol ester induced responses plays a central role in modifying the tPA and PAI 1 secretion from endothelial cells , leading to a profibrinolytic state in the endothelial environment . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cultured BCEs secreted both tissue type and urokinase type PAs ( tPA and uPA ) and PAI 1 into the culture medium , and the secretion of both PAs was enhanced by the addition of bFGF . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , and lipoprotein Lp ( a ) were measured in citrated plasma samples . ^^^ The 214 patients showing significant coronary artery stenosis ( greater than 50 % reduction of luminal area in any of the great coronary arteries ) had higher mean levels of tPA ( P less than 0 . 001 ) and PAI ( P less than 0 . 01 ) than a random population sample of similar age . ^^^ PAI and tPA levels were higher in smokers than in either non smokers or ex smokers , and in patients with hypertension tPA was increased . ^^^ There were positive correlations of PAI and tPA levels with serum triglycerides and with body mass index ; Lp ( a ) correlated weakly with plasma fibrinogen concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following factors were studied in the immediate preoperative period , at different surgical times throughout the procedure and 2 3 h after the end of the abdominal sutures : platelet count , prothrombin concentration , fibrinogen , activated kephalin time , factors 2 , 5 , 7 + 10 and VIIIc , antithrombin 3 , protein C , D dimers , fibrinogen and fibrin degradation products ( PDF ) , plasma plasminogen , tissue plasminogen activator ( tPA ) and the fast tPA inhibitor ( PAi ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Functional determination of tPA , PAI and fibrinogen in endotoxin shock of the pig . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relative immunoreactivity of tPA in free form and tPA in complex with inhibitors was estimated by ELISA and found to be 100 , 74 , 94 , 92 and 81 % for free tPA and tPA in complex with PAI 1 , PAI 2 , alpha 2 antiplasmin and C 1 inhibitor , respectively . ^^^ Addition of tPA to PAI 1 rich plasma resulted in rapid and total loss of tPA activity without detectable loss of ELISA response , indicating an immunoreactivity of tPA in tPA / PAI 1 complex of about 100 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) activity in blood and blood plasma has been difficult to determine because it is unstable , especially in subjects with high levels of plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ During the fast , the basal tPA activity increased from 0 . 34 + / 0 . 21 to 0 . 47 + / 0 . 23 IU / ml ( p = 0 . 05 ) and the PAI 1 activity decreased from 11 . 4 + / 11 . 6 to 6 . 3 + / 8 . 9 U / ml ( p = 0 . 04 ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The method is based on the inhibition of purified tissue plasminogen activator ( tPA ) by plasma diluted with a PAI depleted plasma and the subsequent measurement of residual tPA in the presence of CNBr fibrinogen fragments , purified plasminogen and a plasmin sensitive chromogenic substrate CBS 10 . 65 . ^^^ Under these conditions , parallel titration curves of tPA are obtained in plasma and the values of PAI are reproducible when measured at different dilutions . ^^^ After venous occlusion , variations of PAI were associated with the release of tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 activity and tPA antigen levels were positively associated with triglycerides and inversely associated with HDL cholesterol . ^^^ In multiple regression analysis , however , only triglycerides were found to contribute significantly to the variance of tPA antigen and PAI 1 activity levels , when BMI ( in men ) and abdominal skinfold thickness ( in women ) were entered into the model . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
SMS KAN cells secrete urokinase ( UK ) and tissue PA ( tPA ) as well as a possibly unique PAI . ^^^ Treatment of cells with 1 microM RA resulted in an inhibition of proliferation , extension of neurite like processes indicative of differentiation , as well as a switch from secretion of UK to tPA and a reduction in PAI secretion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , endothelium is involved in the regulation of fibrinolysis by releasing soluble factors , such as tissue plasminogen activator ( tPA ; [ 10 ] ) and plasminogen activator inhibitor ( PAI ; [ 22 , 11 ] ) , as well as in the control of vascular responsiveness by the production of smooth muscle relaxing and contracting factors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Neither fish oil or wheat germ oil caused any significant change in tissue plasminogen activator ( tPA ) or its inhibitor ( PAI ) measured enzymatically or in tPA antigen measured by an ELISA method . ^^^ All these analytes ( tPA , PAI , and tPA antigen ) were measured before and after venous compression . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our data showed that glucocorticoids have a direct stimulating effect on tPA production , but unlike its action on other in vitro systems , they have no significant effect on PAI production by rat granulosa cells in vitro . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma tPA inhibitor complex is precipitated strongly by antisera against plasminogen activator inhibitors ( PAIs ) of human Hep G 2 hepatoma and HT 1080 fibrosarcoma cells and weakly by antiserum against bovine aortic endothelial cell PAI but not by antiserum against a placental PAI ( PAI 2 ) suggesting that the plasma inhibitor is immunologically related to Hep G 2 , HT 1080 and possibly endothedial cell PAIs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Incubation of isolated metabolically labeled substratum with tissue plasminogen activator ( tPA ) resulted in the appearance and release of an immunologically related inactive 44 , 000 Mr form as well as the tPA PAI 1 complex ( 110 , 000 Mr ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In samples drawn 3 months after discharge from hospital , the PAI 1 levels were higher and the tPA activities were lower than among elderly healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Ovarian follicles produce two types of plasminogen activator ( tPA and uPA ) , and their inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our findings suggest that the rate constants for the reactions of PAI with single chain tissue plasminogen activator ( tPA ) , two chain tPA , high Mr urokinase and low Mr urokinase are high and quite similar ( 1 . 6 10 10 ( 7 ) 3 . 9 10 10 ( 7 ) M 1 . s 1 ) . ^^^ However , di isopropyl phosphorofluoridate inactivated tPA inhibited the reaction between PAI and all plasminogen activators in a similar way . ^^^ The reaction rate between PAI and single chain tPA in the presence of an activator substrate ( D Ile Pro Arg p nitroanilide ) was decreased in the presence of fibrin . ^^^ As a consequence , the ' free ' concentration of single chain tPA in the system decreased in the presence of fibrin , affecting the reaction rate between PAI and single chain tPA . ^^^ The phenomenon might be of physiological relevance , in the sense that single chain tPA bound to fibrin in the presence of plasminogen would be protected against inactivation by PAI . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a specific and rapid inhibitor of tissue plasminogen activator ( tPA ) and urokinase . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a specific and rapid inhibitor of tissue plasminogen activator ( tPA ) and urokinase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were positive and independent correlations between tissue plasminogen activator ( tPA ) activity after venous occlusion and HbA1c , and between triglycerides and plasminogen activator inhibitor ( PAI 1 ) and tPA antigen concentrations before and after venous occlusion . ^^^ Tobacco smoking diabetics , as compared to non smoking , had an increased tPA antigen release at venous occlusion , but also higher PAI 1 levels and reduced specific activity of the tPA protein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Purified , two chain mutant tPAs , or analogues of tPA , lacking one or several structural domains , along with Bowes melanoma tPA were studied in order to determine their susceptibility to inhibition by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ The full length analogue of tPA , designated by its domains FGK1K2P , as well as analogues GK1K2P , FK2P , and FGK1P were treated with various amounts of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , alpha 2 antiplasmin ( alpha 2 AP ) and plasminogen are definitely involved in fibrinolysis because : ( 1 ) these components can be assigned a fibrinolytic role in purified systems , i . e . in vitro , and ( 2 ) abnormal structural variants and abnormal levels of these components give rise to bleeding or to thrombosis . ^^^ The cellular regulation compasses synthesis of tPA and PAI 1 and release / uptake of these components . ^^^ The humoral regulation involves : ( 1 ) the reaction between tPA and PAI 1 ; ( 2 ) the fibrin stimulated plasminogen activation ; ( 3 ) the reaction between plasmin and alpha 2 AP and ( 4 ) plasmin degradation of fibrin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
During ergometry the release of tPA antigen did not differ significantly between the age matched groups , however , tissue plasminogen activator ( tPA ) activities after ergometry were higher in groups presenting lower pre test PAI cap values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After 4 weeks of Max EPA intake by coronary artery disease patients , serum triglyceride concentrations and PAI 1 levels decreased 43 + / 8 % and 21 + / 5 % , respectively ( both p less than or equal to 0 . 01 ) without any change in tissue plasminogen activator ( TPA ) levels . ^^^ No changes were noted at 1 week of Max EPA intake in normal subjects , but at 3 weeks serum triglyceride concentrations and PAI 1 levels decreased 32 + / 13 % and 22 + / 4 % , respectively ( p less than or equal to 0 . 01 ) without any change in tissue plasminogen activator ( TPA ) levels . ^^^ No changes were noted at 1 week of Max EPA intake in normal subjects , but at 3 weeks serum triglyceride concentrations and PAI 1 levels decreased 32 + / 13 % and 22 + / 4 % , respectively ( p less than or equal to 0 . 02 ) without change in TPA . ^^^ This study shows that omega 3 PUFA intake reduces PAI 1 levels without change in TPA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Clot lysis of HOTMEC could be blocked by anti tPA IgG but was not affected by the incorporation of exogenous plasminogen activator ( PAI ) into the clot in concentrations ( 75 arbitrary units ) exceeding the tPA activity ( 21 + / 2 . 5 IU ) of the cells . ^^^ Thus , it is likely that tPA secreted by HOTMEC is protected from inhibition by PAI in the presence of fibrin and endothelial cells . ^^^ The stimulation of EC to release an excess of tPA over PAI , in contrast to the secretion of an excess of PAI over tPA found in unstimulated cells in the absence of fibrin , is obviously no prerequisite for the initiation of fibrinolysis on the surface of HOTMEC . ^^^ As thrombin was used for clot formation , its influence on tPA and PAI synthesis of both cell types was investigated . ^^^ In contrast to HOTMEC , which were not affected by alpha thrombin , HUVEC revealed a dose dependent increase in tPA and PAI synthesis upon incubation with the enzyme . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity after a 10 minute venous occlusion was significantly decreased while fibrinogen , factor 8 and von Willebrand factor was increased as well as the tPA inhibitor ( PAI 1 ) : the last 3 proteins are synthetized by the endothelial cell . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After approximately six weeks of catheterization the venographically abnormal patients had significantly less thrombomodulin ( P = 0 . 0055 ) and significantly higher PAI : tPA ( P = 0 . 022 ) in catheter drawn samples as compared with the venographically normal group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured the serial changes of PAI activity and tissue plasminogen activator ( TPA ) antigen after rTPA therapy or direct PTCA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand , as for uPA , tPA is inhibited by PAI 1 . ^^^ The importance of the regulation of this system is illustrated by the thrombotic risk observed when there is either a decrease in tPA or an increase in PAI 1 , and inversely by haemorrhages in the case of increase in tPA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Because of the potential importance of plasmin generation in these processes , we evaluated the effect of elevated glucose concentrations on expression of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) , and urokinase ( uPA ) in cultured bovine brain endothelial cells ( BBEC ) versus cultured bovine aortic endothelial cells ( BAEC ) . ^^^ A decrease in the local tissue activity of PAI 1 by elevated glucose concentrations , with no effect on tPA or uPA expression , would lead to an increase in the plasmin activity and thereby predispose neural tissues , such as the cerebrum and retina , of diabetic patients to neovascularization . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For each sample the plasma levels of betathromboglobulin ( BTG ) fibrinopeptide A ( FPA ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) and D dimer ( D D ) ( ELISA , methods , kits Boehringer ) were measured . ^^^ The basal values of BTG , FPA , tPA , PAI 1 and D D were significantly increased compared to those of a control group ; after the iloprost infusion ( acute effect ) significant changes of the BTG , FPA , tPA and PAI 1 were not found ; D D only showed a marked reduction ( p < 0 . 05 ) ; after the four week treatment with infusion the basal values of BTG , FPA , tPA and PAI 1 resulted almost unchanged ; D D only showed a marked reduction ( p < 0 . 05 ) both as regards the basal value and those after the infusion . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In summary , studies of the expression of fibrinolytic genes in the vessel wall suggest an active , ongoing proteolytic process , the activity of which is dependent on the relative amounts of tPA , uPA , and PAI 1 secreted and locally deposited . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunocytochemical studies indicated that both tPA and PAI 1 positive staining localized in the Sertoli cells , spermatids and residual bodies of the seminiferous epithelium ; Northern blot analysis further confirmed the presence of both tPA and PAI 1 mRNA in monkey Sertoli cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The distributions of urokinase and tissue plasminogen activators ( uPA , tPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) were studied immunohistochemically in two subsets of colorectal adenocarcinomas with low and high aggressiveness , respectively : nine Dukes ' stage A tumors with additional other good prognostic markers and 13 Duke ' s stage C tumors with also other poor prognostic markers ( referred to as Dukes ' stage A and Dukes ' stage C tumors ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Experimental measurements of cardiac output , heart rate , tissue plasminogen activator ( TPA ) , urokinase plasminogen activator ( UPA ) , plasminogen activator inhibitor ( PAI 1 ) , C 1 inhibitor , and TPA / C1 inhibitor complex during the infusions and exercise were used to develop a comprehensive fluid phase model of the circulatory regulation of fibrinolysis . alpha and beta adrenergic agonists increased TPA and UPA in plasma by different mechanisms : Phenylephrine decreased hepatic blood flow and thus clearance while isoproterenol stimulated increased secretion of TPA and UPA . ^^^ C 1 inhibitor contributed equally with PAI 1 to TPA inhibition when active PAI 1 levels were low ( 20 to 50 pmol / L ) but was less important when active PAI 1 levels were high . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : The thrombolytic properties of a new variant of tissue plasminogen activator ( TPA ) ( T103N , N117Q , KHRR 296 299 AAAA , or TNK TPA ) with longer plasma half life , greater fibrin specificity , and increased resistance to inhibition by plasminogen activator inhibitor ( PAI 1 ) were investigated in a rabbit thrombosed carotid artery model . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Conversely , the progression from D 0 to D 15 of the values after occlusion of euglobulin lysis time ( p < 0 . 01 ) , tPA ( p < 0 . 01 ) , and PAI activity ( p < 0 . 05 ) are significantly better in the troxerutin group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although it was not possible to measure uPAR number and affinity it seems likely that elevated uPAR mRNA would translate into increased uPARs which would localize the increased uPA activity to the pericellular region . tPA mRNA and activity were also increased transiently with the activity inhibited with prolonged incubations , apparently by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In comparison with normal control vessels , intimal and neointimal TPA concentrations were reduced in atherosclerotic aortas except in the necrotic core areas of advanced plaques , where TPA was mainly complexed to PAI 1 in extracellular matrix deposits . ^^^ The immunohistochemical distribution of PAI 1 was similar to that observed for TPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In senescent endothelial cells , PAI 1 mRNA and protein are constitutively high , but uninducible by exogenous interleukin 1 alpha as well as by the phorbol ester TPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , the concentration of PAI 1 in the stromal cell conditioned medium at the end of 0 3 days exceeded those of tPA and uPA , respectively , by 28 and 12 fold in response to MPA and by 52 and 25 fold in response to E 2 plus MPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to metabolic abnormalities obese non diabetic subjects and lean and obese NIDDM patients displayed significantly higher levels of fibrinogen , Factor 7 , plasminogen , PAI pre and post VO and tPA ( Ag ) pre VO and significantly lower levels of t PA ( Ag ) post VO . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the current study the authors have investigated the effect of two ACE inhibitors , captopril and enalapril , on tissue plasminogen activator ( tPA ) and PAI in 33 congestive heart failure ( CHF ) patients . ^^^ Blood samples were obtained pretreatment and then at weeks 1 , 12 , and 24 after initiation of ACE inhibitor therapy for measurement of tPA antigen and PAI activity levels . ^^^ The results of the study indicated that the initiation of ACE inhibitors had no significant effects on tPA antigen or PAI activity levels at the time points studied , and no significant differences were demonstrated between captopril and enalapril . ^^^ The results suggest , therefore , that ACE inhibitors do not affect tPA antigen or PAI activity in CHF patients , and thus another mechanism is probably responsible for the observed decrease in coronary artery thromboses in post MI patients treated with ACE inhibitors . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the influence of isotretinoin upon fibrinolysis , and more specifically upon tissue plasminogen activator ( tPA ) and tissue plasminogen activator inhibitor ( PAI ) levels , in haemostatically normal individuals . ^^^ This study provides further evidence that tPA production is stimulated by isotretinoin , and that this , together with falling PAI levels , may accelerate fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
By immunohistochemistry , we studied the expression of PAs ( tPA and uPA ) , their major physiological inhibitor ( PAI 1 ) , and a receptor for uPA ( uPAR ) in human coronary arteries with either pure fibrointimal proliferation ( n = 15 ) or developed atherosclerotic plaques ( n = 10 ) . ^^^ Overall , the degree of staining showed the following rank order : PAI 1 > tPA > uPAR > uPA . ^^^ However , the ratio of intimal to medial expression of tPA ( P = . 001 ) and uPAR ( P = . 004 ) was significantly increased in atherosclerotic arteries , with a similar trend for uPA ( P = . 069 ) but not for PAI 1 ( P = . 73 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN : Immunohistochemical localization of urokinase type PA ( uPA ) , tissue type PA ( tPA ) , type 1 PAI ( PAI 1 ) , and type 2 PAI ( PAI 2 ) in four normal lung biopsy specimens and in four adenocarcinomas ( AC ) , four squamous carcinomas ( SC ) , two large cell carcinomas ( LCC ) , and ten small cell carcinomas ( SCC ) biopsy specimens . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relative topographical distribution of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) , PA inhibitor 1 ( PAI 1 ) , PA inhibitor 2 ( PAI 2 ) , plasmin ( ogen ) , alpha 2 antiplasmin , and alpha 2 macroglobulin was studied in lesional epidermis of psoriasis vulgaris , and in normal epidermis , by immunohistochemistry . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The enzymatic lysis is carried out by glycolytic enzymes and proteases : metalloproteases ( inhibitors TIMP 1 et TIMP 2 ) , serine proteases ( tPA , uPA inhibitors : PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The mean values of plasma protein S , von Willebrand factor ( vWF ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) were normal and were not correlated neither with that of plasma TM nor with that of plasma beta TG . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , we assessed the effects of RU 486 administration on the expression of immunoreactive ( ir ) endometrial stromal cell urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator and their activities as well as levels of ir type 1 plasminogen activator inhibitor ( PAI 1 ) using a well characterized in vitro model of decidualization . ^^^ Compared to the vehicle control , E 2 and RU 486 used alone had no effect on levels of ir PAI 1 , uPA , or tPA or on PA activity in the conditioned medium . ^^^ In contrast , MPA and E 2 plus MPA decreased ir uPA and tPA levels and their corresponding activities , whereas MPA increased , and E 2 plus MPA further increased ir PAI 1 release . ^^^ To determine if RU 486 reversed progestin inhibited stromal cell uPA and tPA release and progestin enhanced PAI 1 expression , confluent cultures were exposed to 10 ( 8 ) mol / L E 2 plus 10 ( 7 ) mol / L MPA for 10 days , washed , and reexposed to E 2 plus MPA , steroid free medium , or RU 486 for 3 5 or 9 11 days . ^^^ Compared with cultures maintained in E 2 plus MPA for 3 5 days , withdrawal to a steroid free medium failed to affect stromal cell ir PAI 1 , uPA , or tPA levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activity of tPA might be inhibited b PAI 1 , although the content of tPA in the culture medium seemed to be increased after endothelial cell injury . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
From a cross sectional population sample of 1558 men and women aged 25 to 64 years , plasma fibrinogen , tPA activity and plasminogen activator inhibitor 1 ( PAI ) activity were determined using specific assays . ^^^ Relationships with PAI 1 activity were essentially the reverse of tPA but stronger . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured three selected components of the fibrinolytic system in amniotic fluid of 36 parturient women , i . e . the antigen of tissue plasminogen activator ( tPA ) and antigens of plasminogen activator inhibitor of type 1 ( PAI 1 ) and type 2 ( PAI 2 ) . ^^^ During labour , the level of each of the studied antigens increases ; tPA antigen increases over five times ( 0 . 91 + / 0 . 32 ng / ml before labour and 5 . 92 + / 1 . 83 ng / ml after childbirth ) , which is statistically significant , while at the same time the increase in PAI 1 and PAI 2 antigen levels is small and thus statistically insignificant . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both tPA and the tPA B chain were largely protected against inhibition by an excess plasminogen activator type 1 ( PAI 1 ) when bound to EC , whereas the same amount of free tPA was totally inactivated . ^^^ This implies a possible competition between PAI 1 and a tPA receptor for binding of tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI ) were determined before and after VOT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of plasminogen activators ( uPA and tPA ) and their inhibitors ( PNI and PAI 1 ) were determined by fibrin zymography , ELISA , amidolytic activity assay , complex formation , and Western blot analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin clot lysis time was related to both tPA and PAI activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) activity and plasminogen activator inhibitor type 1 ( PAI 1 ) activity were used to study fibrinolysis . ^^^ We found no relationship between tPA activity , PAI 1 activity and tobacco use . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , it is unknown whether greater constitutive expression of the tPA gene might increase plasminogen activator inhibitor 1 ( PAI 1 ) secretion , which could negate expected increases in fibrinolytic activity that accompany greater tPA protein production . ^^^ The objective of this investigation was to determine whether transduction of human endothelial cells ( EC ) and vascular smooth muscle cells ( SMC ) with a retroviral vector containing the human tPA gene would increase tPA production and what effect this would have on endogenous PAI 1 secretion and subsequent fibrinolytic activity . ^^^ On days 14 and 28 after transduction , supernatent tPA antigen and PAI 1 antigen levels were measured by ELISA , and supernatent tPA activity was quantitated with a spectrolyse tPA / PAI assay . ^^^ PAI 1 antigen was unchanged in transduced SMC but decreased in MFG tPA transduced EC ( p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Additionally , we have measured the release of urokinase ( uPA ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) into the cell culture media . ^^^ These changes were not significantly different between the two groups . tPA and PAI 1 were not detected in cell lysates or in cell culture media . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
If these more common defects are not found , the rarer defects , including HC 2 , plasminogen , or TPA deficiency , dysfibrinogenemia , elevated PAI 1 , or heterozygous homocystinemia should be looked for . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin lysis time ( ELT ) , a global measure of plasma fibrinolytic activity , and the levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were not different before and after hysterectomy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The influence of acute bouts of dynamic exercise on plasma tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) and fibrinogen was investigated on nine healthy non smoking men aged 23 to 37 years . ^^^ Plasma tPA and PAI activities were measured amidolytically , and fibrinogen concentration by thrombin method . ^^^ The pre exercise tPA and PAI activities and fibrinogen concentration were similar in each tests . tPA activity increased during each test ( from 2 . 0 [ SEM 0 . 24 ] IU / ml to 20 . 3 [ 3 . 14 ] during maximal , 2 . 0 [ 0 . 22 ] to 19 . 0 [ 2 . 59 ] during anaerobic threshold test , 1 . 8 [ 0 . 22 ] to 5 . 5 [ 0 . 82 ] during aerobic threshold ; p < 0 . 001 during all tests , p = 0 . 01 aerobic threshold vs maximal and anaerobic threshold ) . ^^^ One subject had clearly higher pre exercise PAI activity and smaller tPA response to exercise as compared to other subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thirty two percent were found to have prolongation of the postvenous occlusion euglobulin clot lysis time ( PVO ELT ) , 32 % were found to have elevated levels of plasminogen activator inhibitor 1 ( PAI ) and 66 % were found to have reduced release of tissue plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were analyzed for tissue plasminogen activator ( TPA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity was inhibited by actinomycin D and cyclohexamide , but changes in mRNAs for uPA , tPA , PAI 1 , and TF by either cytokine were not appreciable . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( TPA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) activity were measured in 15 inactive , 15 regularly active , and 15 highly active men . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study evaluate both the expression and release of PAs ( uPA and tPA ) and PAIs ( PAI 1 and PAI 2 ) from cultured cells , and also the expression of uPA receptor ( uPAR ) . ^^^ LOX released tPA ( median 9 ng / million cells at 72 hours ) , PAI 1 ( 1050 ng / million cells ) and PAI 2 ( 245 ng / million cells ) , and fibroblasts released uPA ( 1 ng / million cells ) and PAI 1 ( 910 ng / million cells ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate a possible link between endothelial cell damage and VOD , tissue plasminogen activator ( tPA ) and its inhibitor ( PAI 1 ) were measured in 32 patients as endothelial cell derived parameters of the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were quantitated using standard chromogenic activity assays . ^^^ Among all horses , PAI 1 activity was significantly higher and tPA activity was significantly lower on day 2 when compared with other hospital days . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Factor 8 activity ( 8 : C ) , von Willebrand factor antigen ( vWF : Ag ) , tissue plasminogen activator antigen ( tPA ) , plasminogen activator inhibitor activity ( PAI 1 ) , antithrombin 3 , protein C and protein S were assayed before and 1 , 3 , 6 , 12 , 18 , and 24 months after BMT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We determined levels of plasminogen activator inhibitor ( PAI 1 ) antigen and tissue plasminogen activator ( TPA ) antigen in 1431 subjects from the Framingham Offspring Study . ^^^ The between group differences observed for TPA antigen were similar to those for PAI 1 antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There was no modification of PT , PTT , prothrombin fragment 1 + 2 ( F 1 + 2 ) , fXIIC , tPA , PAI 1 , D dimer or protein S levels ; serum levels of tumor necrosis factor alpha were also unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of calcium on human foreskin keratinocyte expression of urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator enzymes and plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) were assessed by Northern analyses . ^^^ Little PAI 1 mRNA and no tPA mRNA were detected , independent of calcium levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activities of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI ) were measured in the peritoneal fluid of rats with faecal peritonitis and correlated with the extent of peritoneal damage to determine the cause of decreased fibrinolysis . ^^^ There was no correlation between the extent of peritoneal damage and tPA or PAI activity . ^^^ Activities of tPA and PAI may originate not only from the mesothelium but from other sources . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A high concentration of glucose alters the production of tPA , uPA and PAI 1 antigens from human mesangial cells . ^^^ To elucidate a role of tPA , uPA and PAI 1 for the development of diabetic glomerulosclerosis , the effect of high glucose concentration on the production of both basal and thrombin mediated tPA , uPA and PAI 1 antigens from human mesangial cells was investigated . ^^^ Thrombin stimulated dose dependently the production of tPA , uPA and PAI 1 from the cells grown in either 5 or 33 mM glucose . ^^^ However , the magnitude of the increase in tPA , uPA and PAI 1 from the cells grown in high glucose was less than that in normal glucose . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study we have demonstrated that both granulosa and theca interstitial cells of hypophysectomized rat ovaries are capable of synthesizing tPA and PAI 1 . ^^^ Both the amount PAI 1 mRNA and its activity in the cells reach the maximum level 6 h before the tPA peak . ^^^ By contrast , granulosa cells produce only a little amount of PAI 1 ( most increase tPA activity ) , and both PAI 1 mRNA and activity in the cells reach the maximum after ovulation . ^^^ The coordinated regulation of tPA and PAI 1 in the ovary may fine tune the peak of tPA activity which may be important for the regulation of the ovulatory process . ^^^ The changes of tPA and PAI 1 in the ovarian cells of hypophysectomized rats during GnRHa induced ovulation are similar to that in intact rats during hCG induced ovulation , suggesting that the ovulatory process can be modulated by different regulatory signals mediated by influencing the coordinated expression of both tPA and PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( TPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activity were measured before and after exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Steady state levels of mRNA for PAI 1 were also decreased by 1 , 25 ( OH ) 2D3 in a dose dependent manner , without significant effects on mRNA for either tissue type PA ( tPA ) or urokinase type PA ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis is mainly dependent on the activity of tissue plasminogen activator ( tPA ) and its inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ In women , the influence of fasting insulin level on tPA and PAI 1 activity was consistently stronger after than before menopause , and a threshold effect was seen with distinctly lower fibrinolytic activity in the highest quartile of insulin ( > 7 . 0 mU / L ) . ^^^ Subjects with previously unknown diabetes or impaired glucose tolerance tended to have elevated fibrinogen and PAI 1 activity and decreased tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The impact of long term , heavy exercise on recently established cardiovascular / thromboembolic risk factors of the fibrinolytic system , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) in relation to food composition was studied . ^^^ Thus , endurance physical activity improves the fibrinolytic risk factor profile by reducing PAI 1 while leaving tPA activity unaffected , independently of food composition . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern blot analysis demonstrated expression of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) and PA inhibitor 1 ( PAI 1 ) in some of the above cell lines . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We investigated tissue plasminogen activator ( tPA ) , its main inhibitor ( PAI 1 ) and the natural anticoagulants protein C and S by enzymimmunoassay prospectively in 32 bone marrow transplant recipients . ^^^ They presented with extremely elevated levels of PAI 1 after BMT whereas tPA levels remained low . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Western blots of Triton extracts incubated with anti tPA antibody demonstrated a major 64 kD protein band , compared to a 70 kD band for standard single chain tPA , plus a minor 118 kD band corresponding to a complex of tPA like PA and plasminogen activator inhibitor ( PAI 1 ) . ^^^ Fibrin zymographic analysis of affinity purified tPA like PA demonstrated a major and a minor fibrin lysis zone , which approximately corresponded to the tPA like PA and its complex with PAI 1 observed by Western blots . ^^^ We conclude that platelets contain a functionally active tPA like PA , whose low fibrinolytic activity might be due to its readily forming a complex with PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( TPA ) antigen , TPA activity , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and PAI 1 activity as fibrinolysis variables , and an angiographic study were performed 2 and 26 weeks after ACBG . ^^^ Exercise training can decrease PAI 1 levels and increase TPA activity after ACBG . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The markers for hemostasis and fibrinolysis were : markers for plasmin generation [ alpha 2 plasmin inhibitor ( alpha 2 PI ) , plasminogen , plasmin alpha 2 PI complex ( PIC ) ] ; markers for fibrinolysis [ fibrin / fibrinogen degradation products E fragment ( FDP E ) , FDP D D dimer ( D dimer ) , fibrinogen ] ; markers for hemostatic activity ( prothrombin time ( PT ) , antithrombin 3 ( AT 3 ) , protein C ) ; markers for thrombin generation [ thrombin antithrombin 3 complex ( TAT ) ] ; markers for intrinsic fibrinolytic activity [ tissue plasminogen activator plasminogen activator inhibitor complex ( TPA PAI complex ) ] . ^^^ The TPA PAI complex level before urokinase administration ( 26 . 4 + / 6 . 4 ng / ml ) was greater than the normal upper limit , indicating increased intrinsic fibrinolytic activity , then decreased after urokinase administration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , gonadotropin and gonadotropin releasing hormone ( GnRH ) regulation of tPA and PAI 1 expression in PMSG primed granulosa cells has been investigated . ( 1 ) Addition of gonadotropins ( FSH and LH ) and GnRH agonist ( GnRHa ) or PMA to the culture increases tPA activity ; FSH ( or LH ) plus GnRHa ( or PMA ) in the culture further enhances the enzyme production to such an extent that a more obvious effect than the additive effect caused by these hormones used alone has been observed ; ( 2 ) in contrast , FSH and LH decrease PAI 1 activity , whereas GnRHa and PMA alone markedly increase PAI 1 mRNA level and PAI 1 activity . ^^^ Because FSH and LH stimulate tPA production and have no significant effect on PAI 1 mRNA induction , the observed inhibition of PAI 1 activity by gonadotropins may be due to the occurrence of neutralization of PA and PAI 1 proteins in the conditioned media by the formation of complexes between PA and PAI 1 ; ( 3 ) increases in PAI 1 mRNA level and activity by GnRH and PMA are completely inhibited by the co addition of FSH or LH to the culture . ^^^ It is , therefore , suggested that the mechanism of gonadotropin and GnRHa regulation of tPA and PAI 1 expression in granulosa cells is different . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relative thrombolysis resistance of the heads of Chandler thrombi can be largely abolished by inclusion of an anti PAI 1 monoclonal antibody that blocks that inhibitory activity of PAI 1 toward TPA . ^^^ This is due to binding of PAI 1 to polymerized fibrin within the thrombus , followed by inhibition of TPA mediated fibrinolysis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI activity was detected in the tunica media , explaining the absence of TPA activity in this layer . ^^^ Removal of the endothelial cells had no effect on TPA antigen and PAI activity in intima media preparations . ^^^ Immunohistochemical staining showed that TPA immunoreactivity was present in all three layers of the aorta , whereas PAI 1 immunoreactivity was found in medial smooth muscle cells but not in endothelial cells . ^^^ Thus , endotoxin decreased TPA activity by increasing the synthesis of PAI 1 ; TPA was unaffected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To screen for risk of embolism , we investigated the association between the presence of left atrial ( LA ) thrombus by transesophageal echocardiography ( TEE ) and blood coagulation tests , including thrombin antithrombin 3 complex ( TAT ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasmin alpha 2 plasmin inhibitor complex ( PIC ) , D dimer , tissue plasminogen activator ( tPA ) , free tPA , tPA . ^^^ Before administration of warfarin , tPA , PA 1 and tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study was the evaluation of the in vitro production of prostacyclin , and of tissue plasminogen activator ( tPA ) and its inhibitor , PAI 1 , by human endothelial cells cultured in the presence of polyethylene terephthalate ( PET ) . ^^^ After a 48 h contact between the cells and the polymer , the concentration of 6 keto PGF 1 alpha , a stable metabolite of prostacyclin , tPA , and PAI 1 , was assayed on the supernatants . ^^^ It can be concluded that the contact between endothelial cells and PET determines a reduction in the platelet aggregability and an increase of the fibrinolytic activity due to a decrease in PAI 1 , while tPA concentration remains unchanged . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern blot analysis revealed that both tPA and plasminogen activator inhibitor 1 messenger RNA levels were elevated at 60 to 120 minutes in well perfused tissues distant from the ischemic insult . ^^^ In addition , peripheral ischemia appears to stimulate both tPA and plasminogen activator inhibitor 1 gene expression in well perfused tissues at later time points , consistent with the existence of humoral mediators . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study was to compare a group ( no . = 38 ) of vascular patients ( aortic aneurysm , carotid obstructions arteriopathies of lower limbs ) and a control group ( no . = 40 ) and assess variations in the following endothelial factors : tPA , PAI 1 , vWf , fibronectin and dimer D , in differentiated degrees according to the type of pathology . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The two types of plasminogen activator , tissue type ( tPA ) and urokinase type ( uPA ) , are produced by osteoblasts , as is the specific PA inhibitor , PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The serpins we analyzed were protease nexin 1 ( PNI ) , PA inhibitor 1 ( PAI 1 , and the kallikrein binding protein ( KBP ) . uPA nearly doubled 48 h after injury , while there was no change in amidolytic activity after addition of fibrin monomer as an estimation of tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( Serpin ) superfamily , is the primary inhibitor of the plasminogen activators tPA and uPA . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( Serpin ) superfamily , is the primary inhibitor of the plasminogen activators tPA and uPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinogen ( Fg ) , plasminogen ( Plg ) , alpha 2 antiplasmin ( alpha 2 AP ) , plasminogen activator ( PA ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , D dimer ( DD ) and fibrin ( ogen ) degradation products ( FDP ) were studied in 60 subjects : 40 patients with endemic hepatosplenomegaly ( 20 during acute haematemesis from ruptured oesophageal varices , 20 with endemic hepatosplenomegaly assigned to the same grade of oesophageal varices but with no history of haematemesis ) and 20 normal controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We immunocytochemically quantified the presence of fibrin , plasmin , TPA , and the TPA inhibitor PAI 1 in 938 biopsies from 68 consecutive cardiac allografts over a 54 month period . ^^^ Biopsies of allografts in the depleted TPA group contained endothelial reactivity for TPA PAI 1 complexes , whereas biopsies from the normal TPA group did not . ^^^ Depleted arteriolar TPA associated significantly with vascular and interstitial deposits of fibrin , plasmin , and endothelial TPA PAI 1 complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue ( tPA ) and urokinase ( uPA ) types of plasminogen activator and the plasminogen activator inhibitor 1 ( PAI 1 ) are enzymatic proteins that may play an important role in the degradation of the extracellular matrix in physiologic and neoplastic conditions . ^^^ We have studied the immunohistochemical distribution of tPA , uPA , and PAI 1 in 24 human gliomas , including seven well differentiated astrocytomas , three oligodendrogliomas , six anaplastic astrocytomas , and eight glioblastomas multiforme . ^^^ Endothelial cells of vessels in non neoplastic and neoplastic brain were immunoreactive for tPA , but not for uPA or PAI 1 . ^^^ Non neoplastic glia were unreactive for tPA , uPA , and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Seminal plasma ( N = 10 ) collected in the absence of extrinsic inhibitors had a mean of 25 + / 5 ng / ml uPA : PCI , 76 + / 23 ng / ml tPA : PCI , and 4 + / 2 ng / ml of tPA complexes with plasminogen activator inhibitor 1 ( tPA : PAI 1 ) . 93 % of the uPA and 17 % of the tPA antigen in seminal plasma was in complex with PCI and , when complexation was inhibited by collecting semen into an 1 , 10 phenanthrolinium solution , 33 % of the uPA and 7 % of the tPA was complexed to PCI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We determined that exposure of cultured lung fibroblasts ( HEL 299 ) to recombinant human interleukin 6 ( 0 400 ng / ml ) resulted in a dose and time dependent increase in secreted and cell lysate PAI 1 and total tPA levels ( maximal increase of 2 . 6 fold and 1 . 7 fold , respectively ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TNK tPA exhibits 80 fold higher resistance to plasminogen activator inhibitor 1 than tPA and 14 fold enhanced relative fibrin specificity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These lines were then analyzed to determine the specific protein ( s ) responsible for differences in PA activity . mRNA and protein levels of cellular uPA , tPA , PAI 1 and PAI 2 were measured using Northern blot analysis and ELISA assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the fibrinolytic system , tissue plasminogen activator ( tPA ) localization and immunoreactivity were comparable in all eyes , but plasminogen activator inhibitor 1 ( PAI 1 ) immunoreactivity was elevated within the walls of retinal vessels in the sickle cell tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were decreases in AT , factors 5 , 2 , 10 , 9 , protein S ( total and free ) , C4b binding protein , thrombomodulin , and platelets counts , whereas heparin , ACT , thrombospondin , plasminogen activator inhibitor ( PAI 1 ) , and tissue plasminogen activator ( tPA ) increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Manual ELISA assays on tPA and PAI 1 automated on Novapath I . ^^^ Tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) have been pointed out as markers for deep venal thrombosis , acute myocardial infarction and several thrombotic disorders . ^^^ S . analyzer ( BioRad , Segrate Milano ) of two manual Elisa procedures on microtritation plates for tPA and PAI 1 ; Tint Elize tPA and Tint Elize PAI 1 respectively ( Biopool Atlanta , Georgia for Italy Menarini diagnostics , Florence ) . ^^^ Automation vs manual procedure allows : 1 ) higher safety for the operator , 2 ) better linearity of the standard curve , 3 ) better analytical precision : 6 . 8 % and 5 . 3 % CV on automation vs 19 . 8 % and 15 . 7 % CV for tPA and PAI 1 respectively , 4 ) simultaneous assays of tPA and PAI 1 , which save time up to 10 hours ( 6 vs 16 hours ) , 5 ) a steady interval between every analytical step , particularly between the interchange of substrate and blocking solution . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Sixteen patients had both abnormalities ( tPA response blunted and high PAI values ) . ^^^ The current pathogeneses of high PAI 1 concentrations and of impaired tPA reactivity to venous occlusion were not found in these patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) and euglobulin lysis time ( ELT ) were measured before and after VOT . ^^^ TPA increased after VOT in both the vegetarians and the non vegetarians , but the changing amplitudes of tPA , PAI and ELT induced by VOT were not significantly different in either group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Four patients had elevated levels of plasminogen activator inhibitor ( PAI 1 ) and one patient had an inadequate increase in tissue plasminogen activator ( tPA ) post venous occlusion . ^^^ These patients should have an evaluation of the fibrinolytic system with tPA and PAI 1 levels as well as a lipid profile . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Defined abnormalities include congenital dysfunction and deficiency of plasminogen , and probably impaired plasminogen activation secondary to elevated levels of plasminogen activator inhibitor type 1 ( PAI 1 ) or to impaired release of tissue plasminogen activator ( tPA ) . ^^^ Patient samples and plasma from 25 normal controls were assayed for tPA activity , PAI 1 activity , and urokinase ( uPA ) activity and antigen . tPA activity and antigen were not significantly different in patients than in controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Factor 8 coagulant activity ( VIIIc ) , von Willebrand factor ( vWf : Ag ) , von Willebrand factor activity ( vWf : RCo ) , tissue plasminogen activator antigen ( tPA ) and plasminogen activator inhibitor activity ( PAI ) was monitored before and after correction of hypothyroidism by hormone supplementation . ^^^ With the correction of hypothyroidism , an increase of tPA was noted in the eleven patients , associated with a significant increase of PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Transcutaneous oxygen tension ( Tcpo 2 ) and tPA , PAI of venous blood were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and von Willebrand factor ( vWF ) , all of endothelial origin and active in the haemostasis , were analysed in 74 patients with rheumatoid arthritis . ^^^ Patients with extra articular disease had a significant increase in PAI 1 activity and reduced tPA release in the venous occlusion test . von Willebrand factor , PAI 1 and also haptoglobin and triglycerides were significantly increased in the group of patients who later suffered from TE . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
By the latter method , we observed a cytotoxic effect on cell growth with 2363 55 D Pellethane extract at a 50 % ( v / v ) concentration in the nutrient medium , likely provoked by leachables and correlated with the lowest levels of tPA , PAI 1 , and vWF antigens in the supernatants . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The balance between the EC derived fibrinolytic components , plasminogen activator ( tPA ) , and plasminogen inhibitor ( PAI 1 ) contributes to maintaining thromboresistance . ^^^ The tPA and PAI 1 antigen and tPA / PAI 1 antigen ratio in conditioned media ( CM ) , expressed as increases or decreases % change , normalized for cell count , versus controls , were analyzed by enzyme linked immunosorbent assay . ^^^ Cellular tPA and PAI 1 mRNAs were assessed by Northern analysis . ^^^ RESULTS : The tPA but not PAI 1 was significantly decreased after the first 24 hours in HSVEC and significantly decreased after 48 hours in both HUVEC and HSVEC when compared with controls . ^^^ Messenger RNA for tPA was unchanged but PAI 1 mRNA increased significantly for HSVEC and HUVEC after 24 hours of Po 2 of 40 mm Hg , returning to baseline within 24 hours of Po 2 to 150 mm Hg restoration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 , Pefabloc TPA , diisopropyl fluorophosphate or alpha 1 anti trypsin inhibit the mitogenic effect of t PA for smooth muscle cells in a dose dependent manner , showing that it is dependent on the enzymatic activity . t PA activated phosphoinositide turnover in smooth muscle cells through a pertussis toxin insensitive pathway and stimulated proto oncogene c fos and c jun mRNA levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we determined the plasma levels of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , urokinase plasminogen activator ( uPA ) , and von Willebrand factor ( vWF ) antigen in Blackfoot disease patients , in comparison with normal controls from non endemic areas and the endemic area , Putai . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic total activity was studied on fibrin plates , tPA and PAI 1 activities by chromogenic method ; tPA and PAI 1 antigens by ELISA technique . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant changes in plasma D dimer and fibrinogen values were detected in the two groups . tPA , urokinase type plasminogen activator , PAI 1 and procoagulant activity were evaluated in vitro on cultured human endothelial cells and found to be unchanged following GM CSF addition . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to elucidate the influence of the risk factors of coronary heart disease on the fibrinolytic activity , relationships between blood pressure , body mass index ( BMI ) , plasma lipoprotein ( a ) ( Lp ( a ) ) level and the plasma levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were analyzed in the subjects with mild hypertension . ^^^ The effects of daily drinking of alcohol and smoking on the fibrinolytic system were also investigated in the present study and we obtained the results that habitual drinking increased plasma levels of both tPA and PAI 1 whereas smoking did not affect fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The mass concentrations and activity of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) in plasma were measured three months after the infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have demonstrated for the first time that ( 1 ) mouse Sertoli cells predominantly secrete tPA under the action of FSH and cAMP generating agents , whereas Leydig cells mainly produce uPA ; ( 2 ) Sertoli cells are also capable of secreting PAI 1 , as well as FSH , growth factors and GnRH increase PAI 1 gene expression ; ( 3 ) the increases in tPA and PAI 1 activities by different hormones in the conditioned media of Sertoli cells correspond to the increases in the levels of tPA and PAI 1 mRNA in the cultured cells , suggesting that the synthesis of the activator inhibitor in mouse Sertoli cells is regulated at a transcriptional level and the tPA secreted by Sertoli cells may be involved in the spermatogenesis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the drug ' s influence on the coagulation and fibrinolytic systems , von Willebrand factor ( vWF ) , the activities of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) , and the split products of cross linked fibrin ( D dimers ) were investigated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Despite normal or high tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) antigen levels , the plasma PAI 1 activity and the formation of tPA / PAI 1 complexes were lower in APL patients than in normal controls , suggesting a proteolytic degradation of PAI 1 , not able to complex tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Assays of the fibrinolytic system included euglobulin clot lysis times ( ECLT ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ This could not be explained either on the basis of tPA levels , which were higher in group 2 when compared to group 1 ( P < 0 . 05 ) and control subjects ( P < 0 . 02 ) , or on the basis of PAI 1 levels which did not differ significantly between the three groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Moreover , uPA , tPA , and PAI 1 were identified in the extracts by immunoenzymatic assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Venous blood samples were taken at time 0 , 3 , 6 , 25 and 49 h for assay of fibrinogen ( Fbg ) , fibrinopeptide A ( FPA ) , total fibrin ( ogen ) degradation products ( TDP ) , fibrin degradation products ( FbDP ) , fibrinogen degradation products ( FgDP ) , cross linked fibrin degradation products ( XL FDP ) , tissue plasminogen activator ( tPA ) , urinary type plasminogen activator ( u PA ) , plasminogen , alpha 2 antiplasmin ( alpha 2 AP ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since platelets are a physiological reservoir for the plasminogen activator inhibitor ( PAI 1 ) contained in plasma , we evaluated plasma and platelet tissue plasminogen activator ( tPA ) and PAI 1 in 20 ET patients with and without thrombotic complications and in 13 control subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In persons with elevated Lp ( a ) , plasma TPA levels were higher than in persons with low Lp ( a ) [ 15 . 7 + / 1 . 5 vs . 11 . 7 + / 1 . 2 micrograms / l ; p = 0 . 051 ] , but plasminogen activator inhibitor ( PAI ) activity and the plasma concentrations of PAI 1 were also higher . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we investigated the binding of tPA on the surface of Bowes melanoma cells , selected since they lacked production of PAI 1 and alpha 2M . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Three major components of the plasminogen activators ( PA ) / plasmin system are synthesized physiologically in glomeruli , and can be involved in glomerular proteolysis and extracellular matrix metabolism : tissue type PA ( tPA ) , urokinase ( uPA ) and PA inhibitor type 1 ( PAI 1 ) . ^^^ RNase protection assays and in situ hybridizations revealed a marked glomerular induction of PAI 1 mRNA abundance without any significant changes in renal tPA and uPA mRNA levels in the two different types of lupus like glomerulonephritis . ^^^ The overexpression of PAI 1 mRNA occurred in parallel with a significant decrease in glomerular tPA catalyzed enzymatic activity as determined by zymographic analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this cross sectional study we compared the abilities of lipoprotein ( a ) [ Lp ( a ) ] , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue plasminogen activator ( TPA ) to discriminate between individuals with and without a history of stroke from among subjects in a metabolic ward . ^^^ Mean plasma TPA and PAI 1 levels significantly ( P < . 001 ) discriminated among subjects younger than 70 years with a history of stroke . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue factor pathway inhibitor ( TFPI ) , a kunitztype inhibitor of the extrinsic coagulation pathway , factor 7 coagulant ( FVIIc ) , FVIIa , and the fibrinolytic factors plasminogen activator inhibitor 1 ( PA 1 1 ) and tissue plasminogen activator ( TPA ) have been studied in various hyperlipidemias . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , we evaluated whether thrombin affected the expression of endometrial stromal cell urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators and their primary inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) , and whether ovarian steroids modulated putative thrombin effects . ^^^ The conditioned medium was then collected and analyzed for immunoreactive ( ir ) uPA , tPA , and PAI 1 by enzyme linked immunosorbent assay and for PA activity by chromogenic assay , whereas Northern analysis of the cells was employed to evaluate the expression of thrombin receptor , uPA , tPA , and PAI 1 messenger ribonucleic acid ( mRNA ) species . ^^^ Thrombin added in the absence of exogenous steroids elevated concentrations of ir tPA , uPA , and PAI 1 compared with control cultures . ^^^ Conversely , in the absence of added thrombin , MPA added alone or together with E 2 inhibited levels of ir tPA and uPA while stimulating PAI 1 levels despite the lack of a response to E 2 alone . ^^^ Interestingly , thrombin counteracted this progestin inhibition of tPA and uPA expression and augmented the progestin enhanced expression of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( TPA ) , free plasminogen activator inhibitor 1 ( free PAI 1 ) , TPA / PAI 1 complex , and total PAI 1 antigen levels were measured before , at 1 week after , and at 3 months after PTCA . ^^^ There were no significant differences in plasma TPA , TPA / PAI 1 complex , or total PAI 1 levels at each sampling period , or in the time courses between the two groups , except for total PAI 1 levels at 1 week after PTCA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Enzyme immunoassays ( EIAs ) showed that urokinase and tissue plasminogen activators ( uPA , tPA ) and PA inhibitors ( PAI 1 , PAI 2 ) were present in ascites from both patient groups and that tPA was the predominant PA . uPA , tPA , and PAI 1 , were detected in plasma from patients and controls . ^^^ The plasmin / antiplasmin and tPA / PAI 1 ratios were particularly high in malignancy related ascites as compared with non malignant ascites . ^^^ Plasma from the liver disease patients showed a higher tPA / PAI 1 ratio and , thus a higher potential for plasminogen activation than plasma from cancer patients and controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Several longitudinal cohort studies of patients with manifest coronary heart disease ( CHD ) have linked elevated plasma PAI 1 or tPA antigen concentrations to future cardiovascular events , particularly myocardial infarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin clot lysis time was significantly prolonged 1 and 4 h after injection and tissue plasminogen activator ( tPA ) activity declined 4 h following administration with a simultaneous increase in its inhibitor ( PAI ) activity . ^^^ Blood 5 HT was negatively related to tPA ( r = 0 . 48 ; p < 0 . 05 ) and positively to PAI activities ( r = 0 . 43 ; p < 0 . 05 ) in tranylcypromino treated rats , whereas there were no such correlations in solvent treated rats . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the patient and control groups , von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) levels were determined using ELISA method and antithrombin 3 ( AT 3 ) by nephelometric methods . ^^^ In the active patient group tPA levels were significantly lower ( p < 0 . 05 ) than the inactive and control groups with higher levels of PAI ( p < 0 . 05 and p < 0 . 01 ) respectively . ^^^ In Behet disease , besides the decrease in tPA synthesis , high PAI levels also can affect tPA decrease and lead to inhibition of fibrinolytic activity . ^^^ In conclusion , high levels of vWF in Behet patients is thought to arise from vasculitis and high levels of PAI from the accumulation of thrombocytes on the damaged surface of endothelium leading to a decrease in tPA levels and inhibition of fibrinolytic activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
While TPA treatment evoked a temporary increased expression of urokinase type PA ( uPA ) , the production of both types of human plasminogen activator inhibitors ( PAI ) was induced and sustained over 12 h by TPA treatment shifting the protease protease inhibitors balance in favor of the inhibitors . ^^^ In addition , inhibition of the PA plasmin system by PAI 2 or aprotinin did not prevent the activation of proMMP 2 by TPA , suggesting that plasmin is not involved in MT MMP mediated activation of proMMP 2 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serial blood samples were obtained via femoral venous catheters for tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) , tPA PAI 1 complex ( tPA PAI ) , and euglobulin lysis time ( ELT ) from all subjects and for fibrin degradation products ( FbDP ) and fibrinogen degradation products ( FgDP ) from phase 2 subjects . ^^^ RESULTS : Catheter placement caused elevations in PAI 1 and tPA PAI , which stabilized within 4 hours of catheter insertion . ^^^ In phase 2 , SGIPC induced significant increases in FbDP , FgDP , and tPA PAI and decreases in ELT and PAI 1 , all of which quickly reverted to baseline on termination of compression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To test the hypothesis that DHEA might exert its effects by enhancing endogenous fibrinolytic potential , a double blind , placebo controlled study was conducted that assessed the effects of DHEA administration on plasma plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) antigen . ^^^ Serum DHEA sulfate and plasma PAI 1 and tPA antigen were measured before and after treatment . ^^^ Plasma PAI 1 and tPA antigen did not change in the placebo group . ^^^ These findings suggest that DHEA administration reduces plasma PAI 1 and tPA antigen concentrations in men . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVES : The aim of this study was to investigate the secretion of prostacyclin ( PGI 2 ) , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) produced by human great saphenous vein endothelial cells ( HSVECs ) in vitro when seeded to confluency on different matrices . ^^^ CONCLUSIONS : The findings emphasise the important effect of the matrix on endothelial secretion of PGI 2 , tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , higher concentrations of the two principal inhibitors of fibrinolysis , PAI 1 and alpha 2 AP , together with lower levels of TPA , are characteristic of advanced atheromatous lesions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the role of plasminogen activators ( tPA uPA ) and inhibitor ( PAI 1 ) in the pathogenesis of preeclampsia . ^^^ STUDY DESIGN : tPA uPA and PAI 1 antigens were measured in amniotic fluid , maternal plasma , and placental homogenates in normal pregnancy by ELISA method and compared with that of preeclampsia . ^^^ RESULTS : In normal pregnancy , tPA , uPA and PAI 1 levels increase as the gestation advance , but the increment of PAI 1 in amniotic fluid ( 28 . 3 fold ) is larger than that of tPA and uPA ( 1 . 8 fold , 8 . 5 fold ) ( p < 0 . 001 ) . ^^^ In preeclampsia , the significantly higher levels of PAI 1 were observed in AF and decidua tissue as compared to the normals ( p < 0 . 01 ) , and the increment of PAI 1 in preeclampsia is larger than that of tPA , uPA . ^^^ CONCLUSIONS : The elevated PAI 1 level is associated with the preeclampsia and the imbalance between the plasminogen activators ( tPA , uPA ) and the inhibitor ( PAI 1 ) might be involved in the pathogenesis of preeclampsia . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The immunohistochemical localization of uPAR , uPA , tPA ) and PAI 1 was evaluated by using the avidin biotin immunoperoxidase technique and affinity purified monoclonal antibodies from American Diagnostica Inc . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antiplasmin and protein C levels are lower in winter in both young and old while plasminogen activator inhibitor ( PAI ) is higher , and tPA higher in old people only . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In pregnant women with HELLP syndrome ( n = 18 ) , pre / eclampsia ( n = 21 ) and highly pathological Doppler flow measurements ( hpD ) ( n = 13 ) , plasma and placental tissue extract concentrations of uPA , uPA receptor , tPA , PAI 1 , MMP 8 , MMP 9 , TIMP 1 , thrombomodulin , and angiogenin were measured using ELISA . ^^^ RESULTS : In all three collectives , PAI 1 plasma concentrations were significantly higher ( p < 0 , 05 ) than in normal pregnancies , in patients with HELLP syndrome , tPA and TIMP 1 plasma levels were also elevated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For a mass study , a tPA PAI 1 complex was formed , composed of non glycosylated PAI 1 and non glycosylated B chain . ^^^ Thus , in the complex between tPA and PAI 1 , the proteins are held together by a tight covalent bond , but the C terminal cleaved peptide of PAI 1 is only bound to the complex by hydrophobic forces . ^^^ To assess whether this is specific to the tPA B chain alone , experiments with the complex of full length , glycosylated tPA and glycosylated PAI 1 were also performed , and it was possible to demonstrate the release of the C terminal PAI 1 peptide by chromatography , mass spectrometry , as well as by SDS PAGE . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We obtained that the fibrinogen , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) levels are significantly increased ( p < 0 . 01 ) in the group of patients , and in the same way , there are also differences ( p < 0 . 01 ) between men and women , so in the control group as in the patients group ; these differences should mean that women have a major risk for suffering an stroke , but it does n ' t fits the fact that stroke is more frequent in men and that they are younger when they suffer from this disease . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this case controlled study , the plasma fibrinolytic system activities in 30 cases of cortical artery territory cerebral infarction ( CACI ) and 32 cases of perforating artery territory cerebral infarction ( PACI ) with a disease duration less than 3 days were assayed with a comprehensive panel including the plasma tPA activity , PAI activity , tPA releasing capacity from vascular endothelium and PAI / tPA ratio values . 30 subjects without cardiovascular and cerebrovascular disorders were served as control . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These findings are discussed along with our previous findings showing normal tissue plasminogen activator ( tPA ) but lower D dimer and higher plasminogen activator inhibitor ( PAI ) levels within the first few hours of life in preterm infants who later developed RDS compared to the control group . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Venous blood samples were taken at each visit for plasma glucose and insulin , HbA1c , triglyceride and cholesterol , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and activity , tissue plasminogen activator ( tPA ) , and euglobulin clot lysis time ( ECLT ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern blot analyses of RNA from the cultured cells demonstrated that the steady state levels of mRNA for uPA , but not for tPA and plasminogen activator inhibitor 1 , were decreased by indomethacin ; this decrease was reversed by prostaglandin E 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A critical component in the regulation of thrombus formation and clearance is the balance between tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ In vitro these compounds reversed the inhibitory effects of PAI 1 against both tPA and urokinase ( UK ) ( IC 50 : 5 to 80 muM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In line with this hypothesis , we have studied , by immunohistochemistry , the distribution of PAI 2 , uPA and tPA in the normal and in the lesional epidermis of patients with lupus erythematosus ( LE ) , a disease in which epidermal differentiation is disturbed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , the plasma fibrinolytic activity in 30 cases of cortical artery territory cerebral infarction ( CACI ) and 32 cases of perforating artery territory cerebral infarction ( PACI ) within 3 days after ictus , and 30 sex and age matched controls without cardio cerebrovascular diseases were evaluated by a comprehensive panel of assay , including the plasma tPA activity , PAI activity , endothelial capacity of tPA release and PAI / tPA ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Concentrations of all antigens ( uPA , tissue type PA ( tPA ) , uPAR , and PAI 1 ) , were significantly greater in RA than OA ; those in OA were significantly greater than normal . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) antigens and activities were measured in 28 patients with hypertension and 12 normal controls . ^^^ As compared to normal controls , hypertensive patients , either with or without insulin resistance , had a significant ( P < . 005 ) increases in PAI 1 activity ( 18 . 6 + / 1 . 3 upsilon 8 . 1 + / 0 . 8 IU / mL ) , PAI 1 antigen ( 31 . 1 + / 2 . 0 upsilon 12 . 7 + / 0 . 9 ng / mL ) and tPA antigen ( 15 . 5 + / 0 . 9 upsilon 8 . 8 + / 0 . 9 ng / mL ) , and significant decrease in tPA activity ( 0 . 43 + / 0 . 05 upsilon 1 . 02 + / 0 . 16 IU / mL ) than normotensive controls . ^^^ Furthermore , hypertensive patients with insulin resistance had significantly higher PAI 1 activity ( 22 . 0 + / 2 . 2 upsilon 15 . 3 + / 0 . 8 IU / mL , P = . 006 ) and tPA antigen ( 17 . 4 + / 1 . 2 upsilon 13 . 6 + / 1 . 3 ng / mL , P = . 02 ) than did hypertensive patients without insulin resistance . ^^^ However , PAI 1 antigen was insignificantly higher ( 34 . 1 + / 2 . 9 upsilon 28 . 1 + / 2 . 4 ng / mL , P = . 06 ) and tPA activity insignificantly lower ( 0 . 42 + / 0 . 08 upsilon 0 . 43 + / 0 . 08 IU / mL , P = . 47 ) in hypertensive patients with insulin resistance than in those without insulin resistance . ^^^ In addition , PAI 1 activity and tPA antigen were significantly correlated with blood pressure , SSPG , triglyceride , HDL cholesterol and integrated glucose response to an oral load of 75 g glucose . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These data suggest that rat Sertoli cells , similar to ovarian granulosa cells , are capable of secreting both tPA and uPA , as well as PAI 1 . ^^^ The interaction of Sertoli cells and Leydig cells is essential for the cells to response to hormone stimulation for tPA and PAI 1 secretion . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since restriction of energy intake has been demonstrated to act beneficially on the aging process in a variety of species , we studied the effect of a 10 week moderately energy restricted ( ER ) regimen ( 80 % of habitual ) on plasminogen activator inhibitor ( PAI ) activity , PAI 1 antigen , tissue plasminogen activator ( tPA ) activity , and tPA antigen in non obese , middle aged men . ^^^ Furthermore , energy restriction led to decreased PAI 1 antigen concentration ( P < . 05 ) , a nonsignificant increase in tPA activity , and a decrease in tPA antigen concentration ( P < . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : A marked increase in the expression of tissue factor ( TF ) and type 1 plasminogen activator inhibitor ( PAI 1 ) and an inhibition of tissue type and urokinase type plasminogen activators ( tPA and uPA , respectively ) , matrix metalloproteinases ( MMP ) , and endothelin 1 ( ET 1 ) expression accompany progestin induced decidualization of estrogen primed endometrial stromal cells both in vivo and in vitro . ^^^ Conversely , progesterone withdrawal reduces TF and PAI 1 expression and increases tPA , uPA , MMP , and ET 1 expression accounting for the hemorrhage , enhanced fibrinolysis , ECM degradation , and ischemic spiral arterial vascular injury characterizing menstruation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The pharmacokinetics of a single intraperitoneal dose of 0 . 5 or 2 . 0 mg / ml human rtPA were assessed in rats with fecal peritonitis and related to their endogenous tPA and plasminogen activator inhibitor ( PAI ) activity . ^^^ Endogenous PAI activity was strongly elevated both in peritoneal fluid and in plasma , whereas tPA activity was only slightly increased in the peritoneal fluid . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Several newer markers for vascular distress , such as tissue plasminogen activator ( TPA ) , tissue plasminogen activator inhibitor 1 ( PAI 1 ) , TPA / PAI 1 complex , and the newly reported inhibitor of the coagulation process , namely , tissue factor pathway inhibitor ( TFPI ) , have been implicated in the pathogenesis of pulmonary hypertension . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since thromboembolic complications in transplanted patients are generally attributed to combined abnormalities in platelets and coagulo lytic system , some hemostatic parameters tPA ( tissue plasmogin activator ) : Ag and activity , its inhibitor PAIAg and activity , tPA / PAI , thrombin antithrombin ( TAT ) and plasmin antiplasmin complexes ( PAP ) , urokinase uPA , euglobulin clot lysis time ECLT , fibrinogen , plasminogen , protein C activity , D dimer , prothrombin fragments1+2 ( F1+2 ) , fibrin monomers , fibronectin , lipoprotein a , and von Willebrand factor ( vWF ) , were evaluated using commercially available kits . ^^^ The TPA level was lower , whereas the PAI level was higher in kidney transplant recipients when compared with controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) increased in several , but not all , cell lines the production of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) as analysed by zymography , enzyme immunoassays and Northern analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This review focuses on the main components of the fibrinolytic system tissue plasminogen activator ( tPA ) , urokinase ( uPA ) and plasminogen activator inhibitor ( PAI 1 ) and on thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Many studies have demonstrated that elevated levels of PAI 1 and tPA antigen are associated with CVD . ^^^ PAI 1 and tPA antigen levels , on the other hand , are closely associated with insulin resistance . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the study was to evaluate fibrinolytic parameters ( antigen of tissue plasminogen activator tPA , its inhibitor PAI , tPA / PAI complexes measured by enzyme immunoassays , euglobulin clot lysis time ECLT ) , cholesterol , triglycerides , lipoprotein ( a ) and apolipoproteins ( AI , AII , B ) in diabetic patients with and without diabetic nephropathy . ^^^ There were no significant differences among the groups studies in tPA : Ag , tPA / PAI complexes , total PAI : Ag and free PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the ability of commercial , chromogenic kits designed to measure human fibrinolytic pathway components to measure the canine plasma fibrinolytic pathway enzymes , tissue plasminogen activator ( tPA ) and plasminogen ( PLG ) , and their respective inhibitors , plasminogen activator inhibitor 1 ( PAI ) and alpha 2 antiplasmin ( AP ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant differences were noted in tPA and PAI levels . ^^^ PAI levels in both patient groups were similar to those of the controls , but tPA levels were higher in patients than in controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using mice with inactivated tissue PA ( tPA ) , urokinase PA ( uPA ) , or type 1 PA inhibitor ( PAI 1 ) genes , we evaluated whether these processes , or their stimulation by parathyroid hormone ( PTH ) or 1 , 25 dihydroxyvitamin ( 1 , 25 [ OH ] 2D3 ) are dependent on these genes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The stimulatory effect was not specific for UPA , in that tissue type PA ( TPA ) also increased migration ( 169 + / 9 % of control , 10 nmol / L , P < . 001 ) ; the augmentation was inhibited by pretreatment with DFP , PAI 1 , or aprotinin and was additive to the UPA effect . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NO metabolites ( NO 2 and NO 3 ) , endothelin ( ET 1 ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) , fibrinogen , D dimer , fibrinopeptide A , beta thromboglobulin ( beta TG ) , blood viscosity , plasma viscosity , haematocrit ( Htc ) and red blood cell ( RBC ) filterability index ( FI ) were determined during headache free periods . ^^^ Migraineurs NO 3 and ET 1 plasma levels , compared to control values , showed a significant decrease and increase respectively ; fibrinogen , beta TG and D dimer appeared slightly lowered in migraineurs , while Htc remained in the normal limits ; tPA , PAI 1 and FI were significantly reduced , while fibrinopeptide A , blood viscosity and plasma viscosity at a low shear rate ( shr ) exhibited a significant rise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activity of tissue plasminogen activator ( tPA ) inhibitor ( PAI ) and PAI : Ag were higher in ARF , whereas tPA : Ag , urokinase , tPA / PAI complexes , thrombin antithrombin complexes ( TAT ) , plasmin antiplasmin ( PAP ) complexes , fibrinogen , and F1+2 did not differ between ARF and controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our main questions were whether and how the concentration of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitors of type 1 ( PAI 1 ) and type 2 ( PAI 2 ) change in the plasma throughout normal labor . ^^^ The concentration of antigens tPA , PAI 1 and PAI 2 in plasma was tested by enzyme linked immuno sorbent assay method , and the activity of PAI 1 by plasmin method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Fourteen patients undergoing laparoscopic cholecystectomy and 12 patients undergoing open cholecystectomy had blood taken in the perioperative period for fibrinopeptide A ( FPA ) prothrombin fragment F1 . 2 , antithrombin 3 , tissue plasminogen activator ( tPA ) and its fast acting inhibitor plasminogen activator inhibitor 1 ( PAI 1 antigen and activity ) , and the euglobulin clot lysis time ( ECLT ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hormone replacement therapy in postmenopausal women has been shown to enhance fibrinolytic capacity by lowering plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator inhibitor ( tPA ) antigen values . ^^^ We evaluated the impact of 4 weeks of estrogen alone ( Premarin 0 . 625 mg / day ) and 2 weeks of estrogen plus progesterone ( Provera 2 . 5 mg / day ) on PAI 1 and tPA in 17 postmenopausal women at multiple time points to assess hormone impact on the diurnal pattern of fibrinolytic potential . ^^^ At baseline , both PAI 1 and tPA exhibited circadian variability . ^^^ Addition of progesterone to estrogen did not reverse effects of the estrogen alone phase of either PAI 1 or tPA values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study attempted to verify the existence of a relationship between oxidative stress documented by malondialdehyde ( MDA ) and superoxide dismutase ( SOD ) and fibrinolysis analysed by tissue plasminogen activator ( tPA ) and its inhibitor ( PAI 1 ) in diabetes mellitus . ^^^ The following were analysed : plasma MDA concentration , SOD activity in erythrocytes , tPA activity and antigen , PAI 1 activity and antigen , fasting blood glucose , fructosamine , glycated haemoglobin ( HbAlc ) , and urine albumin . ^^^ This contrasted with an increased plasma MDA concentration especially in Type 2 diabetes as compared with Type 1 or healthy persons ( p < 0 . 001 ) . tPA activity was increased in both groups of patients with diabetes as compared to healthy persons ( p < 0 . 001 ) , PAI 1 activity was higher in Type 2 diabetes with vascular changes than in the remaining subgroups ( p < 0 . 001 ) . ^^^ Multivariate analysis revealed a significant positive relationship between plasma MDA concentrations and PAI 1 antigen ( r = 0 . 53 , p < 0 . 001 ) and a negative relationship between SOD and tPA activities ( r = 0 . 53 , p < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Sixty one patients with non thrombolytic treated acute myocardial infarction were randomised to open magnesium infusion or control . tPA activity , tPA mass , PAI 1 mass and von Willebrand factor ( vWF ) were measured in blood samples drawn at entrance and after on average 10 h and 18 h following inclusion in the trial . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An increased incidence of events was associated with higher baseline concentrations of tissue plasminogen activator ( TPA ) antigen ( P = . 0002 ) , plasminogen activator inhibitor 1 ( PAI 1 ) activity ( P = . 02 ) , and PAI 1 antigen ( P = . 001 ) . ^^^ CONCLUSIONS : The prognostic role of PAI 1 in predicting coronary events is related principally to insulin resistance , whereas that of TPA antigen could be explained only by its relationship with different mechanisms , including insulin resistance , inflammation and endothelial cell damage . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since TNF alpha may contribute to thrombotic complications in sepsis patients , we determined markers of thrombin activation , parameters of the fibrinolytic system ( D dimer , tissue plasminogen activator antigen ( tPA ) urinary type plasminogen activator antigen ( uPA ) , plasminogen activator inhibitor antigen ( PAI 1 ) and von Willebrand factor antigen ( vWF ) in 30 patients with sepsis or septic shock . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic system is activated during CPB , with raised tissue plasminogen activator ( tPA ) levels and related falls in plasminogen activator inhibitor ( PAI 1 ) . ^^^ However , the patients with the highest tPA surge are not the same patients who have the highest PAI surge . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have developed a first generation kinetic model that can be used to simulate the effects of secretion , inhibition , and clearance reactions on the concentrations of active tPA , active plasminogen activator inhibitor type 1 ( PAI 1 ) , and tPA / PAI 1 complex in the circulation . ^^^ This model was used to estimate the rate and pattern of secretion of tPA and PAI 1 in individual subjects . ^^^ On the first to second postoperative day there was a large increase in PAI 1 activity and a significant decrease in tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study demonstrated the profile of the neutral proteinases , 1 ) matrix metalloproteinases ( MMP ) 1 , 2 , 3 , and 9 , and 2 ) serine proteinases , elastase , cathepsin G , urokinase and tissue type plasminogen activators ( uPA and tPA ) as well as their inhibitors , namely , tissue inhibitor of matrix metalloproteinases ( TIMP ) 1 , alpha 1 antitrypsin , alpha 1 antichymotrypsin , plasminogen activator inhibitor ( PAI ) 1 & 2 , around loose hip prostheses to clarify the step in the cascade of biological host response in the loosening of replaced total hip joints . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen activator system ( PA system ) consists of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) , as well as the two types of plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In an attempt to characterise further the coagulopathy of childhood nephrotic syndrome , this study concentrates on simultaneous measurements of the natural anticoagulants [ antithrombin 3 ( ATIII ) , proteins C and S ] and the fibrinolytic factors , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI ) . ^^^ During relapse , there was a marked increase in the plasma level of fibrinogen , protein C , and protein S and reduced plasma ATIII level ; tPA level was similar to control but PAI level exhibited a significant reduction . ^^^ The fibrinolytic activator tPA dropped slightly but the PAI level remained significantly below control levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Available plasmin , required for MMP zymogen activation , is regulated by plasminogen activators ( uPA , tPA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Invasion is an active process and correlations are observed between cellular invasivness and proteinase production , like uPA , tPA and its inhibitors , for example PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A significant increase of D dimer with time was found ( P < 0 . 001 ) without difference between the two groups , tPA and PAI 1 levels remained stable throughout without difference between the two groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Also the factor 7 coagulant activity ( F 7 : C ) , tissue type plasminogen activity ( tPA ) and plasminogen activator inhibitor activity ( PAI 1 ) were not affected by the experimental diets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : The patients had signs of disturbed fibrinolysis , with elevated plasma levels of tissue plasminogen activator ( tPA ) antigen and plasminogen activator inhibitor ( PAI 1 ) activity at rest , and attenuated responses of tPA antigen and activity to exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Venous blood was collected on fasting from patients and controls to examine the following parameters : fibrinogen ( F ) , factor 7 ( F 7 ) , antithrombin 3 ( AT 3 ) , protein C ( PC ) , protein S ( PS ) using coagulometric methods ( IL ) , and tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) , fibrinopeptide A ( FPA ) , betathromboglobulin ( BTG ) and dimer D ( D D ) using ELISA methods ( Boehringer ) . ^^^ Patients with deep venous thrombosis showed a significant increase in F , FVII , tPA and D D levels compared to controls , whereas a significant reduction was observed in PAI 1 . ^^^ In the light of these results the authors affirm that : high fibrinogen and factor 7 levels are highly prognostic for thrombosis in patients with deep venous thrombosis ; the importance of the lack of inhibitory factors ( AT 3 , PC , PS ) is confined to individual genetically predisposed cases ; there is an efficacious hyperfibrinolytic reactive response to the presence of thrombus ( increase in tPA and D D , reduction of PAI 1 ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of prothrombin fragments 1 + 2 , D dimers , plasminogen , alpha 2 antiplasmin , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI 1 ) in venous blood were investigated just before the operation , at the end of the operation and three hours later . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined the effect of TPA on MMP 9 expression with Northern analysis , and on MMP 9 collagenase activity with polyacrylamide gel electrophoresis zymography , in HT 1080 cells ( positive control ) and in six ovarian cancer cell lines : Hey , HeyA 8 , CAOV 3 , PA 1 , SKOV 3 , and OVCAR 3 . ^^^ RESULTS : PA 1 , a teratocarcinoma , expressed more than 10 fold the amount of RNA for MMP 9 than the other lines following TPA treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate whether smooth muscle cell ( SMC ) proliferation influences overall fibrinolytic properties of the vascular wall , we examined the effect of serial in vitro passaging of human SMCs on tissue plasminogen activator ( TPA ) and PAI 1 synthesis levels as well as the ability to modulate TPA and PAI 1 synthesis of human umbilical vein endothelial cells ( HUVECs ) . ^^^ We observed an increase of PAI 1 secretion ( from 591 + / 106 to 2952 + / 290 ng PAI 1 . 10 ( 5 ) cells 1 . 24 h 1 ) with a concomitant fourfold to fivefold increase of PAI 1 mRNA levels , as well as a decrease of TPA secretion ( from 118 + / 34 to 8 + / 1 . 3 ng TPA . 10 ( 5 ) cells 1 . 24 h 1 ) and a twofold to threefold decrease of TPA mRNA levels with increasing in vitro passage number ( from passage 3 to 11 ) of normal pulmonary artery smooth muscle cells ( PASMCs ) ( P < . 05 ) . ^^^ These data suggest that SMC proliferation is associated with ( 1 ) increased SMC PAI 1 synthesis as well as decreased TPA synthesis and ( 2 ) upregulation of endothelial PAI 1 synthesis by SMC CM . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Novel mechanism to enhance tPA induced fibrinolysis : effect of limited proteolysis of PAI 1 by neutrophil elastase . ^^^ The effect of PAI 1 cleavage by HNE on tissue type PA ( tPA ) induced clot lysis was studied in a purified system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In mice lacking either tissue type PA ( tPA ) or PA inhibitor type 1 ( PAI 1 ) the plasmin activity levels prior to ovulation were similar to wild type mice , while extracts prepared from urokinase type PA ( uPA ) deficient mice had 10 % or less of the plasmin activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In a case control study of 105 cases of transient ischaemic attack and minor ischaemic stroke , and 241 controls , data were available on levels of the haemostatic factors von Willebrand factor ( vWF ) , plasminogen activator inhibitor 1 ( PAI ) , tissue plasminogen activator ( TPA ) and factor 7 ( FVII ) . ^^^ Uncorrected for within person fluctuation , the odds ratio estimates associated with exceeding the median of vWF , PAI , TPA and FVII respectively were 1 . 88 , 0 . 87 , 1 . 30 and 0 . 93 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Human SMC TPA , UPA , and PAI 1 antigen levels were determined by ELISAs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endogenous fibrinolytic activity was assessed by measurement of tissue plasminogen activator ( tPA ) and its naturally occurring inhibitor , plasminogen activator inhibitor ( PAI 1 ) . ^^^ The tPA and PAI 1 antigen ( total protein ) levels were determined using enzyme linked immunosorbent assays , and measurements of in vivo biologic activity were performed using an amidolytic method . ^^^ Measurements of tPA and PAI 1 were made before surgery , after surgery , and on postoperative days 1 , 2 , 7 , and 30 . ^^^ Diminished endogenous fibrinolytic activity in these patients appears to be mediated by a combination of reduced tPA activity and significantly increased PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 concentrations in the same patients were the reverse of their corresponding tPA activities , being higher in those with leukaemia and encephalitis , than in patients with multiple sclerosis . ^^^ Moreover , PAI 1 may play an important role in regulating the functions tPA , and probably uPA , in CSF . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma PAI 1 activity , tissue plasminogen activator ( tPA ) activity , levels of fibrinogen and factor 7 ( c ) , and platelet count were measured before and after intervention ( mean+ / SE ) . ^^^ PAI 1 activity changed similarly in the fish oil and corn oil groups ( 1 . 8+ / 1 . 0 U / mL versus 3 . 5+ / 1 . 2 U / mL , P= . 25 ) , as did tPA ( 0 . 02+ / 0 . 02 IU / mL versus 0 . 13+ / 0 . 03 IU / mL , P= . 28 ) , levels of factor 7 ( c ) ( 6+ / 5 % versus 5+ / 4 % , P > . 3 ) , and platelet count ( 2+ / 7x10 ( 9 ) / L versus 3+ / 5x10 ( 9 ) / L , P > . 3 ) . ^^^ In conclusion , a daily intake of 4 g omega 3 PUFAs does not affect PAI 1 and tPA activity in persons with hypertension . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study was to investigate the changes of fibrinogen , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and lipid levels in continuous ambulatory peritoneal dialysis ( CAPD ) patients with atherosclerosis , and the relationship between those factors and serum albumin . ^^^ MAIN OUTCOME MEASURES : tPA and PAI 1 levels were determined with ELISA method , and fibrinogen with thrombin time method . ^^^ CAPD patients with atherosclerosis had significantly higher fibrinogen , tPA , and PAI 1 levels than other groups : tPA was an independent predictor of atherosclerotic vascular disease in CAPD patients in stepwise logistic regression analysis . ^^^ Serum albumin level was inversely correlated with fibrinogen ( r = 0 . 28 ; p < 0 . 05 ) in CAPD patients , but not with tPA or PAI 1 levels . ^^^ PAI 1 level was correlated with tPA ( r = 0 . 37 ; p < 0 . 01 ) and triglycerides ( r = 0 . 32 ; p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( TPA ) activity and antigen ( ag ) , plasminogen activator inhibitor ( PAI 1 ) activity at test , and TPA responses to exercise were determined at baseline and after 1 month ' s treatment and were related to subsequent fatal and nonfatal CV events . ^^^ Univariate Cox regression analysis revealed that elevated levels of TPA ag at rest ( P < . 05 ) , high PAI 1 activity ( P < . 05 ) , and low TPA ag responses to exercise ( P < . 05 ) were associated with increased risk of subsequent CV death . ^^^ CONCLUSIONS : Plasma TPA ag levels at rest , and among male patients PAI 1 activity as well , independently predict subsequent CV death or MI in patients with stable angina pectoris . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A sensitive and robust assay for urokinase and tissue type plasminogen activators ( uPA and tPA ) and their inhibitor type 1 ( PAI 1 ) in breast tumor cytosols . uPA and PAI 1 are becoming established as amongst the most effective markers of poor prognosis for patients with node negative breast cancer ; tPA is an index of longer survival . ^^^ This paper describes a sensitive ELISA for the measurement of uPA , tPA and PAI 1 in breast cancer cytosols . ^^^ Comparison with the American Diagnostic kits shows the results ' equivalence for PAI 1 and tPA . ^^^ It is the first published assay to yield strictly comparative values for uPA , tPA and PAI 1 in tissue extracts and is readily subject to external quality control . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of lipoprotein ( a ) [ Lp ( a ) ] , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were assessed in addition to anthropometry and levels of glucose , total cholesterol , triglycerides , high density lipoprotein ( HDL ) , low density lipoprotein ( LDL ) and apo A 1 and B in 73 patients ( 36 men and 37 women ) with primary hyperlipidaemia ( group NDHL ) in Kuwait . ^^^ Hyperlipidaemia phenotype ( hypercholesterolaemia or hypertriglyceridaemia ) did not influence tPA and PAI 1 levels , but Lp ( a ) levels were significantly lower with hypertriglyceridaemia . ^^^ Gender , cigarette smoking and racial origin ( Kuwaitis , other Arabs or South Asians ) did not affect Lp ( a ) , tPA and PAI 1 levels , but tPA levels were higher in postmenopausal subjects . ^^^ PAI 1 levels correlated with tPA and triglyceride ( TG ) levels in the groups of subjects ( normo and hyperlipidaemic ) . ^^^ In the normolipidaemic control group , the significant associations of tPA and PAI 1 were with body mass , expressed as the body mass index or the waist hip ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We measured preoperative levels of fibrinogen , cross linked fibrin degradation products ( FDP ) , and the endothelial markers , von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) , in the venous blood of 43 claudicants undergoing percutaneous transluminal angioplasty ( PTA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using in situ hybridization with digoxigenin labelled cRNA probes ( dig cRNA ) , we demonstrated that tPA and plasminogen activator inhibitor type 1 ( PAI 1 ) were expressed in testes of both immature and mature rhesus monkeys . tPA mRNA was localized predominantly in Sertoli cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activator inhibitor 1 ( PAI 1 ) activity and PAI 1 antigen and tissue plasminogen activator ( TPA ) antigen were measured before randomization and on day 14 . ^^^ Clinical characteristics of the three study groups were similar , as were the prerandomization plasma levels of PAI 1 antigen , PAI 1 activity , and TPA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 15 patients we investigated coagulation parameters before , during and post CPB , i . e . , fibrinogen , antithrombin ( AT ) 3 , thrombin antithrombin complex ( TAT ) , prothrombin fragments F 1 + 2 ( F 1 + 2 ) , factor ( F ) XIIa , tissue factor ( TF ) , and parameters of the fibrinolytic system , i . e . , plasmin antiplasmin complex ( PAP ) , D dimer , tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ The enhanced fibrinolytic activity during CPB was , at least in part , caused by tPA and was followed by PAI 1 release . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Fifty patients with SAH caused by ruptured cerebral aneurysms were studied postoperatively to detect the serial changes of the thrombin antithrombin 3 complex , active plasminogen activator inhibitor ( PAI ) 1 , and tissue plasminogen activator ( tPA ) PAI complex ( tPA PAI ) activities in the plasma and CSF collected from cisternal drainage catheters . ^^^ There was no relationship between the CSF and plasma levels of these parameters ( except the CSF levels of tPA PAI ) and the initial neurological statuses . ^^^ The CSF tPA PAI levels showed the highest peak near the time of VS remission . ^^^ The CSF PAI 1 and tPA PAI levels were significantly lower in patients with good outcomes than in those with poor outcomes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Clearance of tissue plasminogen activator ( TPA ) and TPA / plasminogen activator inhibitor type 1 ( PAI 1 ) complex : relationship to elevated TPA antigen in patients with high PAI 1 activity levels . ^^^ BACKGROUND : To evaluate the effect of plasminogen activator inhibitor type 1 ( PAI 1 ) levels on the clearance of total tissue plasminogen activator ( TPA ) antigen , we studied the clearance of active TPA and TPA / PAI 1 complex in subjects with low ( 181+ / 109 pmol / L ; n=7 ) and high ( 1166+ / 322 pmol / L ; n=4 ) baseline active PAI 1 . ^^^ METHODS AND RESULTS : A 5 microg / kg bolus of TPA was infused over a 15 second period followed by measurement of TPA activity , TPA antigen , TPA / PAI 1 , TPA / C1 inhibitor , PAI 1 activity , and PAI 1 antigen over a 4 hour period . alpha Phase clearance of total TPA antigen was faster in subjects with low PAI 1 ( t ( 1 / 2 ) of 3 . 5+ / 0 . 7 minutes ) versus high PAI 1 ( t ( 1 / 2 ) of 5 . 3+ / 0 . 9 minutes ) ( P= . 006 ) . ^^^ The average hepatic clearance fraction in the two compartment model was greater for active TPA ( 89+ / 10 % , t ( 1 / 2 ) of 2 . 4+ / 0 . 3 minutes ) than for TPA / PAI 1 complex ( 48+ / 17 % , t ( 1 / 2 ) of 5 . 0+ / 1 . 8 minutes ) ( P= . 0006 ) . ^^^ CONCLUSIONS : Plasma clearance of active TPA was faster than clearance of TPA / PAI 1 complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
XR 5118 selectively and competitively inhibited binding of the PAl 1 inhibiting monoclonal antibody CLB 2C8 , indicating that binding of XR 5118 to PAI 1 takes place at the area between amino acids 110 and 145 of the PAI 1 molecule , which is known to be involved with the binding of PAI 1 to tissue plasminogen activator ( TPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total homocysteine ( tHcy ) concentrations in culture media as a measure of homocysteine export increased in all cell lines , including the cell line with CS deficiency , with constant amounts of approximately 2 . 5 microM every 24 h . von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) in culture media were used as markers of endothelial function and increased also with progression of culture time . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have used surface plasmon resonance ( SPR ) , the BIAcore , to characterize the binding of a large panel of monoclonal antibodies to four forms of recombinant human PAI 1 , including active and latent PAI 1 as well as the complex between PAI 1 and recombinant human tc tPA or the protease part of tPA , the B chain . ^^^ In addition , in a chromogenic assay with PAI 1 and tPA we determined the IC 50 values for these antibodies , i . e . , studied their ability to inhibit the decrease in tPA activity caused by PAI 1 . ^^^ Furthermore , by using this technique , we have for the first time been able to identify three distinct epitopes on PAI 1 , which are all of importance for the interaction and complex formation with tPA . ^^^ Since the antibodies that bind to one of these areas all have very poor affinity for the complex between PAI 1 and tPA , we suggest that this not previously described epitope must be located near the final binding site for tPA in this complex . ^^^ Altogether , this also supports the theory of a multistep reaction between PAI 1 and tPA , in which tPA interacts with different parts of the PAI 1 molecule . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 587 frozen samples of malignant and nonmalignant tissue of breast , uterus , vulva , and ovary , levels of urokinase plasminogen activator ( uPA ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were examined with enzyme linked immunosorbent assay ( ELISA ) and cathepsin D ( cath D ) with radioimmunoassay . ^^^ In 393 primary breast cancer samples , uPA , PAI 1 , and cath D were not related to other prognostic factors , whereas tPA levels were significantly raised in prognostic more favorable carcinomas . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were significant increases of the von Willebrand factor ( vWf ) , plasminogen activator inhibitor 1 ( PAI ) and tissue plasminogen activator ( tPA ) starting at CPB end and rising to about twice the baseline levels postoperatively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Accordingly , during steroid induced in vitro DZ , a marked increase in the expression of stromal cell TF and PAI 1 and reciprocal inhibition of tPA activity suggest mechanisms to account for the absence of hemorrhage during invasion of the endometrial vasculature by implanting trophoblasts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It also suggests adaptive changes in fibrinolytic potential induced by physical conditioning , as demonstrated by the lower level of tPA at rest and the lower levels of tPA and PAI 1 after exercise in athletes compared to controls . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Amounts of tPA and PAI 1 in the euglobulin fraction obtained at different pH : their relation to the euglobulin clot lysis time . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Global fibrinolytic activity ( euglobulin fraction , fibrin plate assay ) , tissue plasminogen activator ( tPA ) antigen ( Ag ) and activity ( Act ) , plasminogen activator inhibitor 1 ( PAI 1 ) Ag and Act , alpha 2 antiplasmin plasmin complexes , and von Willebrand factor ( vWF ) antigen were assayed . ^^^ The mechanism of increased fibrinolytic activity is likely because of a reduction in PAI 1 , with a resulting increase of tPA activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The tests of fibrinolysis ( serum levels of fibrinogen , D dimers , tissue plasminogen activator ( tPA ) and plasminogen inhibitor 1 ( PAI 1 ) and circulating cytoadhesive molecules ( sE selectin , sP selectin and ICAM 1 ) were followed in a group of 66 pregnant women and compared with the unpaired Student ' s t test with a group of 16 women after uncomplicated ovarectomy for benign disease . ^^^ There were significant correlations between serum levels of 17 beta estradiol fibrinogen ( r = 0 . 46 , p < 0 . 01 ) , plasminogen activator 1 ( PAI 1 ) ( r = 0 . 38 ) and tissue plasminogen activator 1 ( tPA ) ( r = 0 . 22 , p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the plasminogen activators ( tPA , uPA ) and their inhibitors ( PAI 1 , PAI 2 ) at different gestational ages , related to levels in women at term and non pregnant women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : During IRDS we found a systemic activation of both coagulation and fibrinolysis at birth which was represented by lower levels of ATIII ( 27 . 7 + / 8 . 8 % ) and significantly greater levels of TAT ( 37 . 9 + / 31 . 9 ng / ml ) , D dimers ( 1242 . 7 + / 206 . 9 ng / ml ) , tPA Ag ( 10 . 9 + / 5 . 3 ng / ml ) and PAI Ag ( 59 . 9 + / 16 . 7 ng / ml ) than in the control group . ^^^ During their seventh day of life , a significant reduction of all the analysed parameters ( TAT , D dimers , tPA , PAI ) and a significant increase in ATIII were seen in the newborns with IRDS , while no significant modification was observed in the newborns with asphyxia at birth . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured sequential changes in plasma levels of the hemostatic factors ( fibrinogen , fibrin D dimer , and plasminogen activiator inhibitor [ PAI ] ) , markers of platelet function ( soluble leukocyte adhesion molecule P selectin ) and endothelial function ( von Willebrand factor [ vWf ] , soluble thrombomodulin [ sTM ] , and tissue plasminogen activator [ TPA ] ) , and serum lipid levels , including lipoprotein A . ^^^ There was also a trend toward higher median soluble P selectin , PAI , and mean TPA levels and lower vWf levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Factor VIIIc , von Willebrand factor ( vWF ) , tPA and PAI 1 are reported to be associated with CHD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Bulk of tPA activity is in the proximity of the endogenous plasminogen activator inhibitor ( PAI ) as an active complex . ^^^ Enhanced tPA or PAI 1 forms one of the risk factors related to cardiac diseases and thrombotic disorders . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The results of this study indicate that ( 1 ) men with lower FT serum levels have higher fibrinogen and FVII plasma concentrations , and those with lower SHBG serum levels also have higher levels of PAI 1 antigen and activity ; ( 2 ) irrespective of other factors , obesity per se may account for higher concentrations of PAI 1 , fibrinogen , and vWF ; ( 3 ) plasma levels of PAI 1 ( antigen and activity ) and fibrinogen correlate independently with WHR ; and ( 4 ) among the investigated antithrombotic factors ( TPA antigen , protein C , antithrombin 3 ) , only TPA antigen plasma concentrations are higher in men with abdominal obesity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue distribution of uPA , tPA , uPA receptor ( uPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) was studied by immunohistochemistry . uPA , tPA , uPAR , and PAI 1 mRNA values and mRNA distribution were assessed by northern blot and in situ hybridisations respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was performed to compare measurements of the enzymatic components of the intrinsic fibrinolytic system ( tissue plasminogen activator [ tPA ] , plasminogen activator inhibitor [ PAI 1 ] ) with the observed morphologic changes in thrombosed venous segments using venous duplex ultrasound scanning ( VDUS ) at intervals after diagnosis of acute DVT . ^^^ Endogenous fibrinolytic activity was determined at the same intervals by serologic assay of the biologic activities of tPA and its inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients had higher mean levels of tPA inhibitor ( PAI ) than controls ( P < 0 . 001 ) , levels being more elevated amongst patients with diffuse than limited disease ( P = 0 . 01 ) . ^^^ Altogether , these data suggest that patients with SSc are in a hypercoagulable state characterized by elevated plasma levels of FNG and vWF , by a dual hypofibrinolytic pattern ( defective tPA release and elevated PAI ) , and by increased thrombin generation with enhanced fibrin formation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the circulation , fibrinolytic activity is determined to a large degree by the relative levels of tissue plasminogen activator ( tPA ) and its major inhibitor ( PAI 1 ) . ^^^ Vascular beds in different organs secrete tPA and PAI 1 into the circulation , and the total secretory rate of each protein is balanced by its half life in the bloodstream . ^^^ We are testing the hypothesis that in the heart , ventricular hypertrophy will alter the rates of formation of tPA and / or PAI 1 and the rates of their release into the cardiac vasculature . ^^^ In this study , we have examined the effects of continuous hypoxia on PA activity in extracts of rat heart ventricles , on the activity secreted into the cardiac vasculature of perfused hearts , and on the levels of mRNAs for tPA and PAI 1 . ^^^ The mRNAs for tPA and for PAI 1 were elevated after 1 day of hypoxia and then returned to near control levels on days 2 and 3 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The complex of the type 1 plasminogen activator inhibitor ( PAI 1 ) and its target proteinases , the urokinase and tissue type plasminogen activators ( uPA and tPA ) , but not the free components , bind with high affinity to the endocytosis receptors alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( alpha2MR / LRP ) and very low density lipoprotein receptor ( VLDLR ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our study focused on the possible roles of PAI 1 , PAI 2 , and uPA in tPA in myocyte hypertrophy and angiogenesis in the early and late stages of pressure overload induced left ventricular hypertrophy ( LVH ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using in situ hybridization with digoxygenin labelled cRNA probes , we have demonstrated that tPA and PAI 1 mRNAs were localized in epithelial cells of adult monkey epididymis . uPA mRNA was localized in the same areas , but to a much smaller extent . tPA , uPA and PAI 1 mRNAs were greatly expressed in the caput and corpus of adult epididymis than in other regions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Across the whole group , there was a significant inverse relation between arterial PAI 1 antigen levels and increment in TPA activity across the forearm ( r = . 57 , P = . 008 ) but no relation to TPA antigen release . ^^^ At rest , BH subjects had higher circulating PAI 1 antigen levels and a corresponding decrease in circulating levels and local increment of TPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrin D dimer , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI ) have been associated with ischaemic heart disease ( IHD ) . ^^^ D dimer antigen , tPA antigen and PAI activity were measured in stored , fasting plasma samples from 1 , 998 men aged 45 65 examined between 1984 and 1988 , during the first re examination of the Caerphilly Study cohort . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We present a systematic analysis of the sensitivity and specificity of immunohistochemical stainings for components of the plasminogen activation system , i . e . , uPA , tPA , PAI 1 , PAI 2 , and uPAR , on routinely processed ( formalin fixed , paraffin embedded ) tissues . ^^^ For PAI 1 , and to a lesser degree also for tPA , an underrepresentation of stromal cell staining in paraffin material was found , whereas tumor cells showed good staining . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PAI 1 variants have been characterized by their reactivity with tissue plasminogen activator ( tPA ) , interactions with vitronectin or heparin , and stability . ^^^ Most PAI 1 variants , except for Asp 125 > Lys , Phe 126 > Ser and Arg 133 > Asp , displayed a high spontaneous inhibitory activity towards tPA , which did not change greatly on reactivation with 4 M guanidinium chloride , followed by dialysis at pH 5 . 5 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Northern blot analysis showed that tPA mRNA levels were up regulated by estradiol and laminin , whereas PAI 1 mRNA levels were down regulated by laminin and not affected by E 2 . ^^^ Concomitant treatment with laminin and estradiol , decreased PAI 1 mRNA and increased tPA mRNA levels , accounting for the synergistic increase in tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Zymography and Northern blot analysis of purified astroglial , microglial , and mixed glial cell cultures demonstrated that astroglial cells produce tPA and a plasminogen activator inhibitor ( PAI 1 ) and are thereby responsible for the production of plasmin which may activate the inactive TGF in these cultures . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In situ hybridization analysis showed that the distribution and localization of mRNAs for tPA , uPA , PAI 1 and PAI 2 were similar in the fetal membranes of human and rhesus monkey ; no obvious species difference was observed . ^^^ No detectable amount of mRNAs for tPA , uPA , PAI 1 and PAI 2 was found in the fibroblast , reticular and spongy layers . ^^^ Distribution of the proteins of tPA , uPA and PAI 1 in the fetal membranes of these two species was consistent with the distribution of their mRNA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ; 100 nM ) induced the mRNAs for TIMP 1 as well as for MMP 1 , MMP 9 , the uPA receptor , and the uPA inhibitor PAI 1 , amongst which only the responses of MMP 9 and PAI 1 were cell specific . ^^^ The secretion of immunoreactive PAI 1 was considerably elevated ( > 20 fold ) in TPA treated MCF7 / LCC1 cells , whereas the TPA dependent level of 92 kDa MMP 9 was only approximately 2 fold higher in MCF7 / LCC1 cells than in MCF 7 cells . ^^^ These data suggest that TPA responsive in vitro invasive properties that are probably associated with PAI 1 expression may co vary with progression from hormone dependent to independent breast cancer . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The current study was performed to determine whether peripheral arterial disease ( PAD ) and its progression are also associated with impaired fibrinolysis , by measurement of tissue plasminogen activator ( tPA , the activator of fibrinolysis ) and its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Serum levels of tPA and PAI 1 activity were assayed with an amidolytic method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Insulin , cholesterol , triglyceride , HDL cholesterol , PAI 1 activity , PAI 1 antigen and tissue plasminogen activator ( tPA ) antigen . ^^^ RESULTS : PAI 1 activity , PAI antigen and tPA antigen were positively correlated with visceral adipose tissue , but not with subcutaneous adipose tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After intervention , significant between group differences in favor of doxazosin were found : lower triglycerides ( p = 0 . 008 ) and higher HDL cholesterol ( p = 0 . 036 ) ; furthermore , improvement of fibrinolysis : lower plasminogen activator inhibitor 1 ( PAI 1 ) activity ( p = 0 . 012 ) , higher tissue plasminogen activator ( tPA ) activity after venous occlusion ( VO ) ; and higher levels of serum D dimer , both unstimulated ( p = 0 . 0016 ) and after VO ( p = 0 . 0032 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Comparing combined low and usual alcohol periods , an increase in mean weekly alcohol intake from 92 to 410 ml ( mean daily intake from 13 to 58 ml ) was associated with a decrease in plasma fibrinogen ( by 11 % , P < 0 . 001 ) and platelet count ( 3 % , P < 0 . 05 ) , but increases in factor 7 ( 7 % , P = 0 . 001 ) , tissue plasminogen activator ( tPA ; 16 % , P = 0 . 01 ) and plasminogen activator inhibitor 1 ( PAI 1 ; 21 % , P < 0 . 001 ) . ^^^ The ratio , tPA / PAI 1 , fell from 0 . 50 to 0 . 44 ( P = 0 . 02 ) confirming the relatively greater increase in PAI 1 with alcohol consumption . ^^^ The increase in factor 7 and relatively greater increase in PAI 1 than tPA with alcohol intake may attenuate this benefit and indeed may sufficiently predispose individuals to thrombosis to contribute to the increased incidence of ischaemic stroke seen in heavier drinkers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We also measured urokinase type PA ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , which may not be endothelium derived but share major clearance pathways with tPA . ^^^ In M > F transsexuals , mean plasma levels of tPA ( minus 4 . 4 ng / ml ) , big ET 1 ( minus 0 . 8 pg / ml ) , uPA ( minus 0 . 5 ng / ml ) and PAI 1 ( minus 26 ng / ml ) decreased ( all Ps < or =0 . 02 ) . ^^^ In F > M transsexuals , levels of big ET 1 increased ( plus 0 . 4 pg / ml ; P = 0 . 02 ) , while tPA , uPA and PAI 1 did not change ( all Ps > 0 . 25 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of plasma tPA and plasminogen activator inhibitor ( PAI ) 1 and 2 were measured . ^^^ The plasma tPA concentration was significantly higher and the plasma PAI 1 and PAI 2 concentrations were significantly lower in the tPA group than in the control and saline groups on the 7th and 14th postoperative days . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic measures included tissue plasminogen activator ( TPA ) antigen and plasminogen activator inhibitor ( PAI 1 ) activity , and as coagulation factors we examined factors VIIc , factor VIIIc , and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : Blood samples were analyzed for tissue plasminogen activator ( TPA ) activity , TPA antigen , plasminogen activator inhibitor 1 ( PAI 1 ) activity , prothrombin complex F1 . 2 , and antithrombin 3 ( AT 3 ) at the following times : before cardiopulmonary bypass ( CPB ) , during CPB ( 30 and 60 minutes ) , post CPB , and day 1 postsurgery . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tPA and PAI 1 mRNA levels were increased in HDP cells treated with IL 6 , as shown by reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In primary cultured peritoneal mesothelial cells , TGF beta 1 also induced PAI 1 secretion and the shift of tPA toward high molecular weight complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Urokinase type and tissue type plasminogen activators ( uPA and tPA ) and PAI 1 were assayed in vascular SMC conditioned media and in cell lysates , using enzyme linked immunosorbent assay and western blotting . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
K and TNK tPA are more fibrin specific than rt PA , and are also resistant to inactivation by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen activators tPA and uPA , and their inhibitors , PAI 1 and PAI 2 , have been associated with epithelial homeostasis and wound healing . ^^^ In contrast , uPA and tPA mRNA were unchanged over the same time course . uPA , tPA , and PAI 1 mRNA increased in the presence of dbcAMP ; levels remained elevated at least 8 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous studies have demonstrated that low density lipoproteins ( LDLs ) stimulate the production of plasminogen activator inhibitor 1 ( PAI 1 ) and reduce the generation of tissue plasminogen activator ( tPA ) in vascular endothelial cells ( ECs ) . ^^^ The present study investigated the effect of glycated LDL on the production of PAI 1 and tPA in cultured human umbilical vein ECs ( HUVECs ) . ^^^ Glycation increased the abundance of glucitollysine and conjugated dienes in LDL and amplified the overproduction of PAI 1 and the reduction in tPA generation from HUVECs induced by LDL . ^^^ Treatment with 25 mmol / L aminoguanidine , an antioxidant and inhibitor of the formation of advanced glycation end products , during glycation normalized glycated LDL induced generation of PAI 1 and tPA in ECs . ^^^ The results of the present study indicate that glycation enhances the production of PAI 1 and attenuates tPA synthesis in ECs induced by LDL , which may contribute to the increased incidence of cardiovascular complications in diabetes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin autography revealed that both enzymes dose dependently interfered with complex formation between tPA and PAI 1 , making large amounts of tPA remaining to be free form . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Incubation with lovastatin ( 5 microM ) of proximal tubules isolated from untreated rats induced an increase in tPA and uPA and a decrease in plasminogen activator inhibitor 1 ( PAI 1 ) activities . ^^^ In vitro , supernatants , cytosols , and membranes of renal proximal tubular cells in primary cultures had no detectable uPA activity , and lovastatin ( 0 . 1 to 10 microM ) induced an increase in tPA and a decrease in PAI 1 activities and antigens . ^^^ C 3 exoenzyme , an inhibitor of the geranylgeranylated activated Rho protein , reproduced the effect of lovastatin on tPA and PAI activity and blocked its reversion by GGPP . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator ( tPA ) , but reacts with thrombin at a much slower rate . ^^^ PAI 1 contains Glu residues at both the P 4 ' and P 5 ' positions , and previous mutagenesis studies suggest that these residues make productive interactions with Arg 39 of tPA as well as with two other positively charged residues present on the 39 loop of the protease ( chymotrypsin numbering ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator ( tPA ) , but reacts with thrombin at a much slower rate . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
C 3 exoenzyme , an inhibitor of the geranylgeranylated activated Rho protein , reproduced the effect of lovastatin on tPA and plasminogen activator inhibitor 1 activity and blocked its reversal by geranylgeranyl pyrophosphate . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , plasminogen activator inhibitor type 1 ( PAI 1 ) activity was found to be significantly and positively associated with tPA antigen concentration ( P < 0 . 001 ) . ^^^ A multivariate analysis identified chronic low dose aspirin therapy ( P < 0 . 001 ) , PAI 1 activity ( P < 0 . 001 ) , hypertriglyceridemia ( P=0 . 005 ) , the type of angina ( P=0 . 026 ) , multivessel disease ( P=0 . 041 ) , and hypercholesterolemia ( P=0 . 043 ) as significant and independent determinants of tPA antigen . ^^^ The relation of tPA antigen to PAI 1 activity furthermore underlines the relation between tPA antigen concentration and a prothrombotic state . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry and Western blot analysis were performed using an antibody that detects free tPA antigen and tPA complexed to its rapid inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Western blot analysis of brain microvascular proteins confirmed the presence of free tPA ( 67 kDa ) and a stronger band representing tPA PAI 1 complexes . ^^^ In addition to the free pool of tPA , a large portion of tPA is complexed to PAI 1 and is therefore functionally inactive . ^^^ The size of the free tPA cerebrovascular pool may be regulated by PAI 1 , which in turn could suppress fibrinolysis in the cerebral microcirculation . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of this study was to determine the association of tPA antigen levels with CAD and ischaemic stroke and whether associations are independent of levels of PAI 1 antigen . ^^^ In subjects with CAD ( n = 247 ) tPA was associated with the number of coronary arteries with > or = 50 % stenosis , but this association was lost after adjustment for PAI 1 , which was found to be the largest determinant of tPA levels in linear regression models and accounted for as much as 38 % of the variation in levels . ^^^ Levels of tPA were significantly higher in patients with a history of MI compared with those without , even after adjustment for covariates and PAI 1 ( MI : 10 . 0 [ 9 . 4 10 . 6 ] ng / ml ; no MI : 8 . 9 [ 8 . 5 9 . 4 ] ng / ml , p = 0 . 004 ) . ^^^ These data suggest that the associations of tPA with acute thrombosis are independent of levels of PAI 1 but the mechanisms whereby enhanced fibrinolysis may predispose to thrombosis remain unclear . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Biologic variables analyzed included apolipoprotein B ( apo B ) , lipoprotein ( a ) , C reactive protein ( CRP ) , fibrinogen , factor 7 , tissue plasminogen activator ( TPA ) and inhibitor ( PAI 1 ) , thrombin antithrombin ( TAT ) , fragment 1+2 ( F1+2 ) , von Willebrand factor ( vWF ) , activated protein C resistance , homocyst ( e ) ine , anticardiolipin antibodies , blood group , and the angiotensin converting enzyme insertion / deletion ( I / D ) and angiotensin 2 receptor gene polymorphisms . ^^^ These significant correlations were present : age with fibrinogen , homocyst ( e ) ine , and vWF ; factor 7 with apo B , homocyst ( e ) ine , and TPA ; apo B with TPA and CRP ; CRP with fibrinogen , TPA , PAI 1 , and factor 7 ; fibrinogen with vWF . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : To investigate the relation between donor specific fibrinolytic protein genotypes and Tx CAD , we identified donor plasminogen activator inhibitor 1 ( PAI 1 ) HindIII and tissue plasminogen activator ( TPA ) EcoRI restriction fragment length polymorphisms based genotypes by Southern blot analysis in 48 recipients of cardiac allografts and correlated these genotypes with the development of CAD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The rest is enzymatically inactive and consists of a heterogeneous mixture of tPA in complex with inhibitors such as PAI 1 , antiplasmin and C 1 inhibitor . ^^^ The results show that the concentration of the complex between tPA and PAI 1 in plasma correlated strongly with that of tPA antigen in plasma , as measured with a commercially available kit . ^^^ Also the correlation between tPA / PAI 1 complex levels in plasma and the PAI 1 activity concentration was significant . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Plasma levels of modified antithrombin 3 ( ATM ) , tissue plasminogen activator ( TPA ) , its inhibitor ( PAI 1 ) , TPA PAI 1 complexes and plasmin antiplasmin complexes ( PAP ) , d dimer , and fibrinogen were measured in plasma from 36 patients with chronic atrial fibrillation . ^^^ RESULTS : Patients with atrial fibrillation had higher plasma levels of ATM , d dimer , PAI 1 , and TPA PAI 1 complexes than controls ( P < . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We used a blood brain barrier ( BBB ) model to analyze regulation of brain capillary endothelial tPA and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ We measured tPA and PAI 1 mRNA and tPA activity in this model . ^^^ Reverse transcription polymerase chain reaction ( RT PCR ) studies showed similar tPA and PAI 1 mRNA levels after 1 day mono culture ( endothelial cells only ) versus astrocyte endothelial coculture preparations . ^^^ After 7 days ( i . e . , when elements of the BBB are present ) , astrocyte endothelial cocultures ( compared with endothelial mono cultures ) showed a 50 . 7 % + / 27 . 1 % ( mean + / SD ) reduction in tPA mRNA ( P < 0 . 03 ) and a 183 . 3 % + / 86 . 9 % increase in PAI 1 mRNA expression ( P < 0 . 02 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here , we investigated the in vivo effect of theobromine on angiogenic activity of human urothelial cell line HCV 29 , 5 raf transfected ( mouse cutaneous assay ) , and the in vitro effect of this drug on VEGF , tPA , uPA and PAI mRNA expression in these cells ( RT PCR method ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular endothelial and smooth muscle cells synthesize tissue type and urokinase type PA ( tPA and uPA ) and their major physiological inhibitor , PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PAI activity at 4h after the initiation of thrombolysis was significantly lower in the mutant tPA group than in the rtPA group ( 8 . 74 + / 5 . 46IU / L vs 26 . 74 + / 3 . 35 IU / L , p < 0 . 01 ) . ^^^ The mutant tPA gives faster recanalization and lower PAI activity after successful thrombolysis , compared with rtPA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , incubation of mesangial cells 24 h with 100 nM renin provoked an increase of tPA and of PAI 1 in the conditioned medium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : The fibrinolytic capacity showed marked circadian variation at the end of the placebo phase ( p = 0 . 002 ) , plasminogen activator inhibitor 1 ( PAI 1 ) activity peaking at 06 . 00 AM when tissue plasminogen activator ( tPA ) activity was at its nadir . ^^^ Inspection of the 24 h profiles suggested that bisoprolol reduced sympathovagal balance and the morning peak of PAI 1 activity and antigen , with a small increase in tPA activity , although these changes were not significant . ^^^ Quinapril produced a substantial rise in renin ( p = 0 . 01 ) but did not significantly affect either PAI 1 or tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effect of CsA therapy on the regulation of fibrinolysis in kidney graft recipients by measuring plasma concentration and activity of plasminogen activators ( tPA , uPA ) and their inhibitors ( PAI 1 , 2 ) . ^^^ We found an increase in tPA activity and in PAI 1 concentration as well as a decrease in PAI 1 activity in renal allograft recipients as compared to healthy controls , but did not confirm a correlation between these observations and CsA administration . tPA and PAI 2 concentrations as well as uPA activity did not significantly differ between the studied groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study compared plasminogen plasma activity , protein C and protein S plasma activities , fibrinogen and fibrin degradation products ( FGDP and FBDP , respectively ) , von Willebrand factor antigen ( vWF Ag ) , prothrombin fragment F1 . 2 , thrombin antithrombin complexes ( TAT ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) in 16 patients with PPH and in 16 healthy volunteers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CONCLUSIONS : Except for a transient increase in TF expression and reversal of the tPA / PAI ratio , EC exposed to thrombus do not appear to become actively procoagulant . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As assessed by Northern blotting , highly enriched lung MC ( > 98 % pure ) as well as HMC 1 expressed tPA mRNA , but did not express mRNA for PAI 1 , PAI 2 , or PAI 3 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this experiment , the relationship of tPA , PAI 1 and vascular SMC proliferation was studied in vitro using human aortic smooth muscle cells cultivated in normal lipid or high lipid serum . ^^^ PAI 1 inhibits the mitogenic effect of tPA to SMC and is dose dependent . tPA increases oncogene c myc mRNA level during SMC proliferation , and the level of c myc mRNA increases significantly in hyperlipidemia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Tissue plasminogen activator ( tPA ) , its inhibitor ( PAI 1 ) , plasmin antiplasmin complexes ( PAP ) and D dimer were measured in 13 patients with rheumatic atrial fibrillation . ^^^ RESULTS : A significant increase for PAI 1 and D dimer levels were detected in patients with atrial fibrillation group ( p < 0 . 05 ) , with no differences in tPA and PAP concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , the effects of ACE 1 on plasma levels of plasminogen activator inhibitor ( PAI 1 ) and of tissue plasminogen activator ( TPA ) were studied . ^^^ PAI 1 levels in NIDDM were similar to those in control subjects , whereas TPA levels were about 25 % lower in patients than in control subjects ( P = . 013 ) . ^^^ In contrast , plasma levels of cE selectin , cICAM 1 , PAI 1 , and TPA were unaffected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrinogen , coagulation factor VIIc , tPA and plasminogen activator inhibitor ( PAI 1 ) antigen were measured before and after intervention . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present paper we first compared the expression levels of uPA , tPA , PAI 1 and uPAR in a compound group consisting of 33 cancer lesions of various origin ( breast , lung , colon , cervix and melanoma ) as quantitated by ELISA and semi quantitated by IHC . ^^^ Spearman correlation coefficients between IHC results and ELISA results for uPA , tPA , PAI 1 and uPAR varied between 0 . 41 and 0 . 78 , and were higher for the compound group and the breast cancer group than for the melanoma group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) rapidly inactivates tissue plasminogen activator ( tPA ) . ^^^ We propose that the antibodies which convert PAI 1 into a substrate for tPA do this by means of preventing the conversion of the initial PAI 1 / tPA complex into the final complex by sterical intervention . ^^^ Moreover , the localisation of the binding epitopes on free PAI 1 , as well as on the PAI 1 / tPA complex , suggests that tPA in the final complex can not be located near helices E and F , as has previously been proposed . . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) rapidly inactivates tissue plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Studies carried out over the past 10 years in a number of laboratories have elucidated some of the biochemical events related to the function and regulation of the PA system in the ovary : hormone induced proteolytic activity provided by tissue type PA ( tPA ) and modulated by PAI 1 in the preovulatory follicles is responsible for a controlled and directed proteolysis leading to rupture of selected follicles during ovulation , whereas the coordinated expression of urokinase type PA ( uPA ) and PAI 1 in the early growing follicle may be important in ECM degradation during cell proliferation and migration ; the PA system may also play a role in the control of corpus luteum ( CL ) development through an autocrine or paracrine mechanism . ^^^ Increase in tPA and PAI 1 expression in CL at a later stage is well correlated with a sharp decrease in CL progesterone production , while the increase in uPA mRNA levels and activity in the early stage of CL development is correlated with an increase in progesterone secretion . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
ELISA for complexes of urokinase type and tissue type plasminogen activators with their type 1 inhibitor ( uPA PAI 1 and tPA PAI 1 ) . ^^^ An ELISA has been developed for the assessment of complexes between the urokinase type ( uPA ) and the tissue type plasminogen ( tPA ) activators with their inhibitor type 1 ( PAI 1 ) in cell culture medium and cytosolic extracts of breast tumours . ^^^ Analogously , the tPA PAI 1 complex was assessed using the formats tPA / PAI 1 and PAI 1 / tPA . ^^^ Comparable results have been observed when tPA , PAI 1 and tPA PAI 1 values were measured in tumour biopsy extracts . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Abnormal response to methionine loading was associated with higher tissue plasminogen activator ( tPA ) mass concentration , higher plasminogen activator inhibitor 1 levels , and lower tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : The role of plasminogen system components in focal cerebral ischemic infarction ( FCI ) was studied in mice deficient in plasminogen ( Plg / ) , in tissue or urokinase plasminogen activator ( tPA / or uPA / ) , or in plasminogen activator inhibitor 1 or alpha 2 antiplasmin ( PAI 1 ( / ) or alpha 2 AP / ) . ^^^ CONCLUSIONS : Plg system components affect FCI at 2 different levels : ( 1 ) reduction of tPA activity ( tPA gene inactivation ) reduces whereas its augmentation ( PAI 1 gene inactivation ) increases infarct size , and ( 2 ) reduction of Plg activity ( Plg gene inactivation or alpha 2 AP injection ) increases whereas its augmentation ( alpha 2 AP gene inactivation or alpha 2 AP neutralization ) reduces infarct size . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To demonstrate the utility of this assay as a screen for thrombolytic agents , a 14 amino acid PAI 1 inhibitory peptide relieved the PAI 1 effect on tPA in a dose dependent fashion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Specific monkey cRNA and antibodies against human tPA , uPA , PAI 1 and PAI 2 were used as probes . ^^^ It is possible that both decidual and extravillous trophoblast cells of placentae of human and rhesus monkey are capable of producing tPA , uPA , PAI 1 and PAI 2 to differing extents . ^^^ The differences in distribution we observed are consistent with the suggestion that coordinated expression of tPA and its inhibitor PAI 1 may play a key role in fibrinolytic activity in the early stages of placentation and separation of placenta from maternal tissue at term . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Tissue plasminogen activator antigen ( tPA ) and tissue plasminogen activator inhibitor 1 ( PAI 1 ) activity were measured in 20 normotensive male subjects and 46 male patients with mild essential hypertension divided into 2 groups ( 22 patients treated with 5 to 10 mg enalapril once a day and 24 treated with 5 to 10 mg nitrendipine once a day ) before and 3 months after drug administration . ^^^ Before drug treatment , the 2 hypertensive groups had significantly higher tPA and higher PAI 1 activity than the normotensive subjects . ^^^ In the enalapril group , there was no significant change in tPA although PAI 1 activity significantly decreased after drug treatment . ^^^ In the nitrendipine group , there was no significant change in tPA although PAI 1 activity significantly increased after drug treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Rarer defects include heparin cofactor 2 ( HC 2 ) , plasminogen or tissue plasminogen activator deficiency ( TPA ) , elevated plasminogen activator inhibitor 1 ( PAI 1 ) and dysfibrinogenemia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After further adjustment for baseline insulin levels , leptin levels were in males significantly associated with increased waist circumference ( P < 0 . 001 ) , low HDL cholesterol ( P < 0 . 05 ) , low tPA activity ( P < 0 . 01 ) and high PAI 1 activity ( P < 0 . 001 ) . ^^^ In postmenopausal females , a significant association between leptin and low tPA activity / high PAI 1 activity was seen after adjustment for age and BMI ( P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our observations are consistent with this idea and suggest that the equilibrium involves partial insertion of the RCL into sheet A : latent , RCL cleaved , and tPA complexed PAI 1 , which are inactive loop inserted forms , bound much faster than active PAI 1 to MA 33B8 , whereas two loop extracted forms of PAI 1 , modified to prevent loop insertion , did not bind or bound much more weakly to the antibody . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in urokinase plasminogen activator ( uPA ) , tissue like plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) immunoreactivity were also assessed . ^^^ Endothelial tPA immunoreactivity decreased significantly after irradiation ( P < 0 . 001 ) , whereas uPA and PAI 1 immunoreactivity levels appeared to be unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of tPA , may contribute to arterial thrombolysis resistance . ^^^ We used a murine carotid injury model to test the hypothesis that PAI 1 inhibits thrombolysis mediated by pharmacological concentrations of tPA . ^^^ Mean plasma tPA antigens were 2 . 7 microg / mL ( tPA infusion , 20 microg . kg 1 . min 1 ) and 5 . 5 microg / mL ( tPA infusion , 100 microg . kg 1 . min 1 ) , with no significant differences between PAI 1 + / + mice and PAI 1 / mice . ^^^ Reperfusion after tPA 20 microg . kg 1 . min 1 occurred in 1 of 5 PAI 1 + / + mice versus 5 of 5 PAI 1 / mice ( P=0 . 0006 ) . ^^^ Reperfusion occurred in all mice that received tPA 100 microg . kg 1 . min 1 , but reperfusion times were significantly shorter in PAI 1 / mice ( 17 . 8+ / 2 . 6 minutes , n=6 ) than in PAI 1 + / + mice ( 35 . 7+ / 5 . 1 minute , n=6 ; P=0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In stable patients ( n = 40 ; 39 males 1 female ; 51 74 years old ) who previously had undergone coronary artery revascularization ( 1 10 years ) , no alterations in prothrombin time , international normalized ratio ( INR ) , prothrombin fragment 1 . 2 , or the hemostatic risk factors factor 7 antigen and coagulant activity , von Willebrand ' s factor , fibrinogen , tPA , or PAI 1 were associated with the 1 mg daily warfarin treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An impaired fibrinolytic function , as evidenced by increased plasma concentrations of PAI 1 , tPA antigen and tPA / PAI 1 complex , or a decreased capacity to release active tPA on exercise , is more common in individuals suffering from myocardial infarction . ^^^ There is a highly statistically significant correlation between tPA / PAI 1 complex and both PAI 1 and tPA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : A series of hemostatic tests and parameters including activated partial thromboplastin time , prothrombin time , thrombin time , bleeding time , factor 8 assay , antithrombin activity , fibrinogen , plasminogen , protamine sulfate test , fibrin ( ogen ) degradation products , D dimer , thrombin antithrombin complex ( measured by modified antithrombin ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 , euglobulin lysis test and venous occlusion test were performed in 51 patients with cirrhosis of the liver and 33 healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fasting insulin concentration was correlated inversely with HDL cholesterol and directly with the fasting triglyceride concentration , blood pressures , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue plasminogen activator ( tPA ) , but it was not correlated with LDL cholesterol , apolipoproteins B and A 1 , lipoprotein ( a ) , ( Lp ( a ) ) , fibrinogen , factor VIIc , or prothrombin fragment ( F ) 1 + 2 . ^^^ Indians had lower HDL cholesterol , and higher PAI 1 , tPA , and Lp ( a ) , but not higher LDL cholesterol , fasting triglyceride , blood pressures , fibrinogen , factor VIIc , or prothrombin F 1 + 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For hemostatic proteins of predominantly extrahepatic origin ( von Willebrand factor . tPA , PAI 1 ) this tendency was not present . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These effects were shown to extend to the physiological tPA inhibitor , plasminogen activator inhibitor 1 . tPA . plasminogen activator inhibitor 1 complex did not compete tPA binding to VSMC , and the inhibition of bound tPA was reduced by 30 fold . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In type 2 diabetes mellitus elevated tPA antigen levels in early disease stage are caused by increased production of complexes with inhibitor ( PAI 1 ) and tPA or fibrinolytic activity has been decreased . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The ratios of active over total TPA were increased several fold in extracts of advanced lesions despite a concomitant threefold increase in TPA complexed to its inhibitor PA 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) , a member of the serine protein family , is the most active in vivo inhibitor of fibrinolysis induced by plasminogen , tissue plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The expression of tissue factor ( TF ) , tissue factor pathway inhibitor ( TFPI ) , von Willebrand factor ( vWF ) , endothelial nitric oxide ( NO ) synthase ( eNOS ) , tissue plasminogen activator ( tPA ) , its inhibitor ( PAI 1 ) , and myosin , an indicator of local shear stress , was examined in the endothelium of cerebral vessels according to vessel size and location in human autopsy brains , using immunohistochemistry . ^^^ Expression of TF , vWF , eNOS , tPA / PAI 1 , and myosin was much greater in intracerebral perforating arteries and the microvasculature than the pial and carotid arteries . ^^^ However , TF , vWF , myosin , tPA , and PAI 1 were strongly expressed in the endothelium of the inner wall of the carotid bifurcation where flowing blood collides , but not in the outer wall . ^^^ In the endothelium of arteries with fibrillary hyperplasia , vWF , myosin , eNOS , tPA , and PAI 1 were strongly expressed . ^^^ However , expression of TF and myosin was more intense in the basal gray matter and white matter than in the cortex . eNOS was expressed more strongly in the basal gray matter and cortex than the white matter , whereas tPA and PAI 1 expression was more intense in the white matter than the gray matter . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Body weight , oxygen consumption , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , tissue plasminogen activator ( tPA ) antigen , fibrinogen and fasting plasma insulin measured at the start of the programme and at follow up after 1 year . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Angiotensin converting enzyme ( ACE ) inhibition significantly decreases plasminogen activator inhibitor 1 ( PAI 1 ) without altering tissue plasminogen activator ( tPA ) during activation of the renin angiotensin aldosterone system in humans . ^^^ Plasma PAI 1 antigen , tPA antigen , plasma renin activity , and aldosterone were measured in 25 normotensive subjects ( 19 white , 6 black ; 14 men , 11 women ; mean age 38 . 5+ / 1 . 8 years ; mean body mass index 25 . 3+ / 0 . 7 kg / m ( 2 ) ) during low salt intake alone ( 10 mmol Na / d ) , low salt intake + quinapril ( 40 mg PO bid ) , and low salt intake + losartan ( 50 mg PO bid ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 76 HD patients ( prevalent CVD , 44 of 76 patients ) , serum lipid , lipoprotein , apolipoprotein ( Apo ) , plasma fibrinogen , tissue plasminogen activator ( TPA ) , plasminogen activator inhibitor ( PAI 1 ) , and factor 7 levels were measured using standard kits . ^^^ Serum lipid , lipoprotein , Apo , plasma TPA , PAI 1 , and factor 7 values did not differ significantly from laboratory norms or discriminate for prevalent CVD in HD patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tPA activity , tPA antigen and tPA inhibitor 1 ( PAI 1 ) antigen were determined in the supernatant of transduced ( BBEC / tPA ) cell cultures by an immunoassay . ^^^ The PAI 1 antigen was not affected by tPA cDNA transfer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There is evidence that serine proteases , such as tissue plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor ( PAI ) , are involved in this process . ^^^ Vitreous levels of VEGF , TPA , UPA , and PAI were determined by enzyme linked immunosorbent assay . ^^^ RESULTS : We found a statistically significant correlation between levels of VEGF and TPA ( P < 0 . 01 ) and VEGF and PAI ( P < 0 . 026 ) in the vitreous fluid of patients with PDVR . ^^^ Concentrations of VEGF ( P < 0 . 03 ) , TPA ( P < 0 . 042 ) , and PAI ( P < 0 . 0098 ) in diabetic eyes were significantly higher than in nondiabetic eyes . ^^^ CONCLUSIONS : A correlation between increased levels of VEGF and TPA or PAI in the vitreous fluid of eyes with PDVR reflects the possible role of plasminogen activators in the progression of this disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in the expression of this system , consisting of urokinase and tissue type plasminogen activators ( uPA and tPA , respectively ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) and uPA receptor , have been associated with tumour aggressiveness in a variety of solid malignant tumours . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Gene expression for plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase and tissue plasminogen activators ( uPA and tPA ) , urokinase plasminogen activator receptor ( uPAR ) , and transforming growth factor beta 1 ( TGF beta 1 ) was examined by Northern analysis . ^^^ RESULTS : At 6 weeks , when fibrosis had occurred , uPA and uPAR mRNAs had increased 2 . 8 fold and 1 . 8 fold , respectively ; PAI 1 and tPA mRNA levels were unchanged . ^^^ At the cirrhotic stage ( 9 to 12 weeks ) , mRNA levels for PAI 1 , uPA , uPAR and tPA were all increased . ^^^ CONCLUSION : Though increased uPA and uPAR may act on matrix degradation in fibrotic liver , increased PAI 1 together with uPA , uPAR and tPA are associated with overall inhibition of matrix degradation in cirrhotic liver . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the production by leukaemic cells of plasminogen activators [ urokinase ( uPA ) and tissue type PA ( tPA ) ] , cell surface receptor for uPA ( uPAR ) and PA inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In RT PCR experiments , tissue type PA ( tPA ) mRNA levels in both aged hGF and rGF were higher than in young cells , whereas plasminogen activator inhibitor 1 ( PAI 1 ) mRNA remained unchanged and urotype PA ( uPA ) mRNA was not detected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic PMC activity was detected by zymography for tissue plasminogen activator ( tPA ) and by reverse zymography for plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In both cell types , the secretion of PAI 1 was stimulated by bFGF and PDGF , as well as by uPA and tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : We evaluated assays to measure both total tissue plasminogen activator ( tPA ) and the three principle forms of tPA in plasma : active tPA , tPA complexed with plasminogen activator inhibitor type 1 ( PAI 1 ) , and tPA complexed with C 1 inhibitor . ^^^ METHODS : Active tPA was measured by use of an indirect amidolytic assay and immunofunctional assays . tPA / PAI 1 , tPA / C1 inhibitor , and total tPA antigen were measured by use of microtiter plates coated with anti tPA antibodies and , respectively , anti PAI 1 , anti C 1 inhibitor , and anti tPA antibodies conjugated to peroxidase . ^^^ RESULTS : The immunofunctional tPA assay detected 1 U / L ( 0 . 001 U / mL ) tPA and recovered 108 % + / 12 % of active tPA added to samples containing high ( mean , 60 000 IU / L ) PAI 1 activities vs a detection limit of 10 U / L ( 0 . 01 U / mL ) and 13 % + / 25 % recovery for the indirect amidolytic tPA activity assay . ^^^ For measurement of tPA / PAI 1 complex , polyclonal anti PAI 1 conjugates recovered 112 % + / 20 % of the expected tPA / PAI 1 vs recovery of only 38 % + / 16 % when monoclonal anti PAI 1 conjugates were used . ^^^ A single anti tPA capture antibody that performed the best in the individual assay evaluations was used to measure the different forms of tPA in 22 samples with a range of tPA and PAI 1 values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was designed to test whether plasma levels of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tPA / PAI 1 complex could predict a first ever stroke . ^^^ The mean plasma concentration of tPA / PAI 1 complex was higher for the stroke cases than for the controls ( 3 . 9 versus 3 . 0 microgram / L ) . ^^^ In univariate regression analysis , significantly higher odds ratios were found for the tPA / PAI 1 complex as continuous variable . ^^^ In the multivariate model , the tPA / PAI 1 complex remained an independent predictor for stroke . ^^^ In subgroup analysis of cerebral hemorrhage ( n=18 ) , the mean tPA / PAI 1 complex level was higher for the cases than for the controls ( 4 . 8 versus 3 . 0 microgram / L ) , and in multivariate analysis including all controls ( n=216 ) , only tPA / PAI 1 complex remained significant . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increasing levels of alcohol consumption were associated with increases in tPA and plasminogen activator inhibitor ( PAI 1 ) activity and with decreases in fibrinogen and white cell count . tPA , PAI 1 , fibrinogen ( nephelometric ) , and viscosity were positively associated with body mass index . ^^^ Use of prescribed medicines ( a marker for chronic illness ) was associated with adverse levels of D dimer , fibrinogen , plasma viscosity , and white cell count . tPA , PAI 1 , and plasma viscosity were associated with blood pressure , cholesterol , and triglycerides but not with lipoprotein ( a ) or homocysteine . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Von Willebrand factor ( vWF ) and the fibrinolytic factors tissue plasminogen activator ( tPA ) , measured as tPA capacity , and plasminogen activator inhibitor 1 ( PAI 1 ) , platelets , fibrinogen , and inflammatory markers were measured in 74 patients with active seropositive RA . ^^^ In a multiple logistic regression model controlling for several conventional cardiovascular risk factors and pharmacological treatment at sampling , PAI 1 and tPA were significantly associated with cardiovascular disease progression . ^^^ CONCLUSION : The altered levels of vWF , PAI 1 , and , in logistic regression , tPA in RA patients with cardiovascular disease progression indicates a status of hypofibrinolysis in these patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This effect was blocked by plasminogen activator inhibitor 1 , by tPA STOP , and by co incubation with tPA antibody . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At each time interval , tissue plasminogen activator ( tPA ) and tPA inhibitor 1 ( PAI 1 ) antigens and activities and total euglobulin fibrinolytic activity were determined and portal vein flow velocity , volume , and congestion index were measured by duplex Doppler . ^^^ Total fibrinolytic activity showed a similar pattern , which was statistically significant also in controls . tPA and PAI antigens also showed significant circadian rhythm both in controls and cirrhotics , with higher values in the morning . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On both occasions plasma total cholesterol , LDL C , HDL C , triglycerides , lipoprotein [ a ] ( Lp [ a ] ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , antithrombin 3 ( AT 3 ) , plasminogen and fibrinogen were determined just before the meal ( t 0 ) and at times 2 hours , 4 h , 6 h , 8 h after it ( t 2 t8 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To study this supposition , we investigated immunohistochemically the expression of tPA , uPA and its receptor , the plasminogen activator inhibitors PAI 1 and PAI 2 , tetranectin as well as the laminin breakdown as an event of secondary brain injury . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Aged hPLF cells produced a significantly higher PA activity when compared with those of young hPLF cells in response to MTF in a time and magnitude dependent manner . tPA mRNA levels in aged cells were higher than those in young cells , whereas PAI 1 mRNA remained unchanged and uPA mRNA was not detected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coagulation fibrinolysis parameters determined were D dimer ( DD ) , thrombin antithrombin 3 complex ( TAT ) , tissue plasminogen activator ( TPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of TPA , PAI 1 and thrombospondin in patients with systemic vasculitis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand , the Y99L mutation has no effect on the activity of tPA toward the natural substrate plasminogen , that carries Gly at P 2 , and reduces more than 10 fold the inhibition of tPA by plasminogen activator inhibitor 1 ( PAI 1 ) , that carries Ala at P 2 . ^^^ Mutations of Y 99 could therefore afford a resistance to inhibition by PAI 1 without compromising the fibrinolytic potency of tPA , a result of potential therapeutic relevance . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
None of the patients had elevated tissue plasminogen activator ( tPA ) antigen levels , but PAI activity was markedly decreased in all cases . ^^^ There tended to be a reduction in tPA PAI 1 complex in all cases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Adult human saphenous vein endothelial cells ( HVEC ) were cultured in a compliant tubular device and evaluated by Northern hybridization for the effects of combined pressurized pulsatile flow and cyclic strain on the expression of mRNAs for endothelin 1 ( ET 1 ) , endothelial cell nitric oxide synthase ( ecNOS ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Only oral ethinyl estradiol plus CA but neither transdermal 17beta estradiol plus CA , nor oral CA , nor parenteral testosterone lowered plasma tPA and plasminogen activator inhibitor 1 ( P < 0 . 001 for both ) . tPA release during venous occlusion was not affected by oral ethinyl estradiol plus CA in males ( P=0 . 52 ) or by parenteral testosterone in females ( P=0 . 89 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma fibrinogen , antithrombin 3 , von Willebrand factor , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) prothrombin time , and activated partial thromboplastin time were determined using standard methods . ^^^ RESULTS : Plasma levels of the procoagulant proteins fibrinogen ( grade 1 : 3 . 7 / grade 2 : 3 . 9 / grade m : 4 . 0 g / l ) and vWF ( 158 / 156 / 178 % ) increased and of antithrombin 3 ( 109 / 103 / 102 % ) and the PAI 1 / tPA ratio ( 5 . 2 / 5 . 0 / 4 . 1 ) decreased with progressive disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , the levels of secreted plasminogen activator inhibitor 1 ( PAI 1 ) were markedly higher , while no apparent differences were seen in the levels of active uPA and tPa between EGF R ( / ) and wild type pancreata . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , no significant differences between the two periods were seen in factor 7 coagulant activity ( FVIIc ) , fibrinogen , prothrombin fragments 1+2 ( F1+2 ) , tissue plasminogen activator ( tPA ) plasminogen activator inhibitor 1 ( PAI 1 ) or 2 , 3 , dinor thromboxane B 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before and after treatment , glycemic control , steady state plasma glucose and insulin ( SSPG and SSPI , respectively ) , and markers of fibrinolysis ( tissue plasminogen activator [ tPA ] and plasminogen activator inhibitor 1 [ PAI 1 ] ) were analyzed in each patient . ^^^ Pretreatment plasma PAI 1 in diabetic patients , but not tPA , was well correlated with the severity of retinopathy assessed by the fluorescence technique . ^^^ Four weeks of treatment with troglitazone significantly decreased hemoglobin A1c ( HbA1c ) , SSPG , and PAI 1 without an alteration of tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since reported data have indicated that plasminogen activators ( uPA and tPA ) were able to excise the angiostatin fragment from the plasminogen parent molecule via plasmin generation , we determined levels of uPA and tPA and PAI 1 antigen in the conditioned media , and correlated the results with angiostatin generating capacity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Elevated levels of plasminogen activator inhibitor 1 ( PAI 1 ) with and without reduction of tissue plasminogen activator ( tPA ) in plasma have been frequently found in patients with DM . ^^^ Our previous studies indicated that glycation enhances low density lipoprotein ( LDL ) induced production of PAI 1 and further decreases tPA generation in vascular endothelial cells ( ECs ) . ^^^ The present study demonstrated that treatment with antioxidants , butylated hydroxytoluene or vitamin E , blocked native LDL and glycated LDL induced changes in PAI 1 and tPA generation in ECs . ^^^ Cotreatment with native or glycated HDL inhibited LDL induced or glycated LDL induced changes in PAI 1 and tPA generation in ECs . ^^^ The effects of antioxidants and HDL on LDL induced or its glycated LDL induced changes in the generation of PAI 1 and tPA were also found in cultured human coronary artery ECs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both isoforms ( alpha and beta ) of IL 1 ( interleukin 1 ) increased as cells approached crisis , and the presence of these cytokines may be responsible for the increased levels of tPA , PAI , and uPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We assessed insulin sensitivity ( 5 h , 80 mU 10 m ( 2 ) 10 min ( 1 ) hyperinsulinemic euglycemic clamp ) and measured plasma PAI 1 antigen and activities and tissue plasminogen activator ( tPA ) in 14 patients with type 2 diabetes and 20 normal control subjects ( 10 lean , 10 obese ) before and after 3 months of treatment with troglitazone ( 600 mg / day ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Co treatment with the serine protease inhibitors aprotinin or plasminogen activator inhibitor 1 also suppressed the cytostatic activity of TPA approximately 50 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the status of hemostatic factors , fibrinogen , tissue plasminogen activator ( tPA ) antigen level , plasminogen activator inhibitor 1 ( PAI 1 ) antigen level , and PAI 1 activity were assessed . ^^^ However , tPA antigen level , fasting and postload plasma glucose and insulin , total cholesterol , triglycerides , WHR , and BMI did not correlate with PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The defects found were as follows : antiphospholipid syndrome , 67 % ; sticky platelet syndrome , 21 % ; tissue plasminogen activator ( TPA ) deficiency , 9 % ; factor 5 Leiden , 7 % ; high PAI 1 , 6 % ; protein S , 5 % ; high LP ( a ) , 3 % ; AT , 2 % ; protein C , 1 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An impaired fibrinolytic function due to elevated plasma levels of plasminogen activator inhibitor ( PAI ) 1 activity or tissue plasminogen activator ( tPA ) antigen is correlated with the development of myocardial infarction ( MI ) in patients with manifest coronary heart disease . ^^^ The hemostatic compounds studied ( fibrinogen , von Willebrand factor , tPA antigen , PAI 1 , and the tPA / PAI 1 complex ) were typically higher in the groups ( men and women ) with recurrence of MI compared with those without . ^^^ The largest between group differences were found for the plasma tPA / PAI 1 complex . ^^^ The crude odds ratio for reinfarction associated with higher concentration ( > / =75th percentile among the control subjects ) of tPA / PAI 1 was 1 . 8 ( 95 % CI 1 . 1 to 3 . 1 ) ; the corresponding crude odds ratio for von Willebrand factor was 2 . 3 ( 1 . 3 to 4 . 0 ) . ^^^ The tPA / PAI 1 complex correlated strongly with PAI 1 and tPA antigen in all groups and with serum triglycerides and body mass index in all groups except for women with reinfarction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For this purpose , the presence and enzymatic activity of matrix metalloproteinases ( MMP 2 , MMP 9 ) , tissue inhibitors of MMPs ( TIMP 1 , TIMP 2 ) , urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators ( PAs ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were quantified by western blot and gelatin or plasminogen casein zymography . ^^^ Levels of PAs ( uPA and tPA ) as well as PAI 1 were both lower in varicose veins ( p < 0 . 005 ) , with minimal change in the PAI / PA ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An immunohistological study showed that the fibrin layer was positive for tPA , but weakly positive for PAI 1 . ^^^ Some multi nucleated giant cells were immunostained for MMP 7 and 9 , tPA , PAI 1 , uPA , and uPAR . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In particular , pravastatin at a dosage of 20 mg / day significantly reduced only fibrinogen levels , while at a dosage of 40 mg / day significantly reduced factor 7 , fibrinogen , prothrombin fragments 1 and 2 , thrombin antithrombin complexes , tissue plasminogen activator antigen ( tPA : Ag ) before venous occlusion ( b . o . ) , inhibitor of plasminogen activator activity ( PAI ) b . o . , PAI activity after occlusion ( a . o . ) , the human autoantibodies against oxidized low density lipoprotein ( LDL ) , and the c fraction of the third component system levels , and significantly increased tPA : Ag a . o . levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentrations of plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) as measures of the fibrinolytic activity of mesothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We compared two methods that measure plasminogen activator inhibitor ( PAI ) activity in plasma based on the ability of PAI to inhibit tissue plasminogen activator ( tPA ) or urokinase ( uPA ) in order to determine which method most accurately measures plasma PAI activity after stressors , like hemorrhage . ^^^ Using standard curves derived from rhPAI 1 , we found that the tPA PAI assay was more sensitive than the uPA PAI assay . ^^^ However , we measured a 10 fold difference in PAI activity as measured between assays , suggesting that some endogenous plasma constituents ( tPA , uPA , plasminogen or plasmin ) may interfere with the accurate determination of PAI activity . ^^^ However , removing either tPA or plasminogen from the tPA PAI assay unmasked the presence of some endogenous tPA and plasminogen . ^^^ These results suggest that endogenous tPA and plasminogen may interfere with the measurement of plasma PAI activity in the tPA PAI assay after hemorrhage or other stresses . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this paper we describe the expression of the tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and the uPA receptor ( uPAR ) , in normal and atheromatous human vascular tissue obtained at coronary and peripheral vascular surgery . tPA , uPA , PAI 1 and uPAR antigens were localised by immunohistochemistry . ^^^ Vessel homogenates were used to quantitate tPA , uPA and PAI 1 antigens as well as uPA and PAI 1 activities using immunoassay and immunoactivity assays , respectively . ^^^ In situ hybridisation ( tPA , uPA and PAI 1 ) was used to localise mRNA . ^^^ In normal saphenous vein or internal mammary artery , expression of tPA , uPA and PAI expression is associated with endothelium and with intimal or medial smooth muscle cells , but expression is at a low level . uPAR protein was seen on the endothelium of normal saphenous vein or internal mammary artery but absent on the smooth muscle cells . ^^^ In complex atheroma tPA , uPA , PAI and uPAR proteins were associated with the endothelium , groups of smooth muscle cells ( in the intima and around vascular channels , but not with the media ) , infiltrating mononuclear cells , and also with acellular areas . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples taken the morning of the operation and the following morning were analyzed for TAT , FM , fragment 1+2 , tPA , PAI 1 , vWf , D dimer , Hb , hematocrit , and APC resistance . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fluvastatin treatment had no significant effect on creatininemia , plasma cyclosporine , PAI 1 antigen , PAI 1 activity , tPA antigen , and Von Willebrand factor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The interaction of fibrinolytic components with GPIb / V / IX of platelets on thrombus formation , was investigated in mice deficient in tissue type ( tPA / ) , urokinase type plasminogen activator ( uPA / ) or plasminogen activator inhibitor 1 ( PAI 1 / ) and in their wild type control ( tPA+ / + , uPA+ / + , PAI 1+ / + ) . ^^^ The times to occlusion after the initiation of endothelial injury in all wild type mice was within 12 min , and no significant changes in occlusion delay were observed in uPA / and tPA / mice compared to wild type mice , whereas that of PAI 1 mice were significantly prolonged ( 16 . 9+ / 2 . 9 min , P < 0 . 05 ) . ^^^ In conclusion , the antithrombotic effect of inhibition of platelet GPIb / V / IX is severely affected by the absence or presence of tPA production on thrombus formation and the inhibition of PAI 1 could enhance this antithrombotic effect . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate whether the course of primary melanoma disease correlates with expression of the various components of the proteolytic plasminogen activation ( PA ) system , immunohistochemical stainings for activators of plasminogen ( tissue type ( tPA ) and urokinase type ( uPA ) ) , inhibitors of plasminogen activation ( type 1 ( PAI 1 ) and type 2 ( PAI 2 ) ) and the receptor for uPA ( uPAR ) were performed on 214 routinely processed melanoma lesions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were collected at the beginning and at the end of each dietary period from fasted subjects for determination of factor 7 coagulant activity ( FVIIc ) , activated factor 7 ( FVIIa ) , factor 7 antigen ( FVIIag ) , tissue plasminogen activator ( tPA ) activity , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , fibrinogen , prothrombin fragment 1+2 ( F ( 1+2 ) ) , and plasma lipids . ^^^ There were no significant differences between diets in fasting plasma concentrations of FVIIc , FVIIa , FVIIag , fibrinogen , F ( 1+2 ) , PAI 1 activity , and tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The following parameters were evaluated in 104 patients with previously untreated hypertension : plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) antigen , fibrinogen , D dimer , and von Willebrand factor ( vWF ) . ^^^ RESULTS : Those with DD genotype ( n = 42 ) had significantly higher plasma levels of PAI 1 antigen ( P = . 012 ) , tPA antigen ( P = . 0001 ) , fibrinogen ( P = . 0002 ) , D dimer ( P = . 0001 ) and vWF ( P = . 0004 ) compared with ID ( n = 30 ) or 2 ( n = 32 ) genotypes . ^^^ The ACE gene genotypes appeared to be significant predictors for plasma PAI 1 antigen , tPA antigen , fibrinogen , D dimer , and vWF even after adjustment for age , sex , body mass index , triglyceride and cholesterol levels , and blood pressure . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Subjects with the metabolic syndrome were characterised by increases in PAI 1 activity , tPA antigen , protein C and protein S and low concentrations of tPA activity . ^^^ Insulin sensitivity was independently and reversibly associated with PAI 1 ( p = 0 . 014 ) and directly with tPA activity ( p = 0 . 001 ) . ^^^ Protein C and protein S were significantly associated with PAI 1 activity , tPA activity ( negatively ) , tPA antigen and antithrombin 3 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , the role of the individual activators ( urokinase [ uPA ] and tissue plasminogen [ tPA ] activators ) and inhibitors ( plasminogen activator inhibitor [ PAI 1 ] ) of the fibrinolytic system in maintaining patency after coronary artery angioplasty and stenting is unclear . ^^^ Antigen and activity assays were performed for uPA , tPA , and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We assessed plasminogen activator ( uPA and tPA ) and inhibitor ( PAI 1 ) expression in rat iliofemoral arteries after balloon injury using Western blot , enzyme activity , and quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin autography showed that plasminogen dependent fibrinolytic activity occurred at M ( r ) of 110 kDa , which represents a complex of tPA with PAI 1 , and 65 and 55 kDa bands corresponding to tPA and uPA , respectively . ^^^ These observations demonstrated that tPA and PAI 1 are produced and secreted by LEC monolayer cultures and are also present in lymph . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma concentration of PAI 1 ag , PAI 1 activity , fibrinogen , tPA 1 akt , tPA ag , von Willebrand factor , fasting plasma insulin , and the lipid pattern ( cholesterol , TG , HDL , LDL ) was measured . ^^^ There was direct correlation between PAI 1 act . , Fibrinogen and BMI in both groups of patients together and in premenopausal group PAI 1 act correlated directly and tPA ag / act . indirectly with plasma insulin concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Deprivation of these two amino acids decreased the secretion of urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) while plasminogen activator inhibitor ( PAI ) 1 and 2 were increased in these cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activated factor 10 levels decreased significantly by 11 . 3 % ( P < 0 . 01 ) and the ratio of tPA / PAI 1 ( 0 . 146+ / 0 . 07 ) increased significantly by 37 . 7 % from baseline ( P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentrations of the fibrinolytic components tPA and urokinase plasminogen activator ( uPA ) , tPA activity and plasminogen activator inhibitor type 1 ( PAI 1 ) concentration were measured by enzyme linked immunoabsorbent assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Acute psychological stress decreases plasma tissue plasminogen activator ( tPA ) and tissue plasminogen activator / plasminogen activator inhibitor 1 ( tPA / PAI 1 ) complexes in cardiac patients . ^^^ The effects of acute laboratory stressors on tPA and tPA / PAI 1 complexes were assessed in a sample of 11 cardiac patients . ^^^ Two way repeated measures analysis of variance revealed a statistically significant decrease in tPA ( P = 0 . 01 ) and tPA PAI 1 complexes ( P = 0 . 04 ) during the mental stress condition . ^^^ Anger in had a strong relationship to decreases in tPA / PAI 1 levels in the stress condition ( r = 0 . 68 , P < 0 . 05 ) . ^^^ Relaxation had no significant effect on tPA and tPA / PAI 1 levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antitrombin 3 ( ATIII ) , fibrinogen degradation products ( FDP ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) have been evaluated preoperatively and 6 , 12 , 24 and 48 hours after the operation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Baseline levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI ) were elevated in eight and 14 patients respectively . tPA increased from 12 . 6 + / 5 . 2 ng / ml to a maximum of 19 . 0 + / 6 . 7 ng / ml at 72 h . ^^^ Elevation of PAPc correlated with elevation of tPA and reduction of PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes of plasma tPA and PAI activities in patients with diabetic retinopathy . ^^^ PURPOSE : To investigate the relationship between the diabetic retinopathy ( DR ) and the activity of plasma tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI ) . ^^^ METHODS : tPA and PAI activities were measured by chromatogenous substrate assay in plasma samples obtained from patients with and without diabetic retinopathy ( n = 42 ) . ^^^ CONCLUSIONS : Reduced plasma tPA activity and enhanced PAI activity in diabetic patients , probably is one of the causes for thromboembolic diseases and are related to the severity of DR and duration of diabetes . ^^^ Therefore , the change and imbalance between activities of plasma tPA and PAI might play a role in developing and progression of DR . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIMS : To plasminogen activator system ( PAS ) consists of the plasminogen activators ( urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators ) , the uPA receptor ( uPAR ) , and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , after the incubation of PAI 1 with SP 40 , 40 at 37 degrees C for 1 h , PAI 1 could still form a complex with tissue plasminogen activator ( tPA ) , and it inhibited plasmin formation in the mixture of plasminogen and urine plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( tPA ) , urokinase ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) and inhibitor 2 ( PAI 2 ) in bile were measured by enzyme linked immunoassay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although it had no manifest impact on cellularity or differentiation of skin or hair follicles , PAI 2 overexpression rendered the mice highly susceptible to skin carcinogenesis induced by a single application of 7 , 12 dimethylbenz ( a ) anthracene ( initiation ) followed by twice weekly applications of 12 O tetradecanoylphorbol 13 acetate [ TPA ( promotion ) ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Deprivation of these two amino acids decreased the secretion and protein expression of tissue type plasminogen activator ( tPA ) while expression and secretion of plasminogen activator inhibitor ( PAI 1 and PAI 2 ) were increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentrations of lipids , lipoproteins , homocysteine , factor 7 , fibrinogen , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) 1 , soluble intercellular adhesion molecule ( ICAM ) 1 , P selectin and interleukin ( IL ) 8 were not beneficially affected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was designed to investigate whether prolactin ( PRL ) affects coordinated regulation of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) gene expression in rat granulosa cells in vitro . ^^^ Several methods , such as SDS PAGE , immunoblot etc . were used to detect the effect of PRL on tPA and PAI 1 gene expression . ^^^ The synergistic increase in PAI 1 mRNA levels occurs in a dose and time dependent manner ; ( 2 ) the increase in PAI 1 mRNA synthesis in the cells by PRL alone , or PRL in combination with FSH , is well correlated with the changes of PAI 1 activity levels in the conditioned media ; ( 3 ) PRL in culture also decreases FSH induced tPA activity level in a dose dependent fashion . ^^^ The decrease in FSH induced tPA activity level by PRL is correlated with an increase in the amount of PA PAI 1 complexes in the conditioned media . ^^^ This suggests that the decline of tPA activity is related to neutralization of tPA by the increased PAI 1 activity in the media . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Impedance cardiogram and pheopneumogram , blood gas analysis , mean systemic arterial pressure ( mSAP ) , plasma tissue plasminogen activator ( tpA ) and plasminogen a ctivator inhibitor ( PAI ) activity were examined before and after treatment . ^^^ RESULTS : After treatment , the mPAP of QNOL group lowered for 23 . 80 + / 2 . 31 % , the cardiac output , stroke volume and cardiac index of patients were improved , tPA activity elevated and PAI activity decreased . ^^^ The change of mPAP showed negatively correlated with tPA activity ( r = 0 . 554 ) and positively correlated with PAI activity ( r = 0 . 608 , P < 0 . 05 ) . ^^^ CONCLUSIONS : QNOL has a selective effect on pulmonary hypertension , its mechanism might be correlated to the elevating plasma tPA and the lowering of PAI activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
D dimer was significantly decreased ( 0 . 38 ng / L vs 0 . 28 ng / L , P = 0 . 02 ) and tPA , PAI 1 and fibrinogen tended to decrease after therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urinary albumin excretion was significantly associated with body mass index , systolic and diastolic blood pressure , plasminogen activator inhibitor ( PAI ) 1 activity , tissue plasminogen activator ( tPA ) antigen , tPA activity ( negatively ) and protein S ( P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the level of plasma tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , 6 keto prostaglandin F 1 alpha ( PGF 1 alpha ) , thromboxane B 2 ( TXB 2 ) , factor 8 related antigen ( 8 R : Ag ) in acute cerebral infarction ( ACI ) patients with different TCM syndrome type and the therapeutical effect of activating blood stasis ( ABS ) . ^^^ RESULTS : ( 1 ) The activity of tPA , ratio of tPA / ( tPA + PAI ) and level of PGF 1 alpha decreased significantly , the level of 8 R : Ag increased remarkably in ACI patients than those of the controlled subjects . ( 2 ) Between Differentiation of Deficiency and Excess Syndromes , the level of above index changed more remarkably in the case with Excess Syndrome . ^^^ However , the improvement of index such as level of 8 R : Ag , ratio of tPA / ( tPA + PAI ) and TXB2 / PGF1 alpha showed significant change between the beginning and end of treatment of DF 521 with HBM group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
D dimer is emerging as a strong risk marker , as are several fibrinolytic markers such as tPA antigen but not PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , tPA and PAI 1 remained unaltered , except in the patients with thrombosis in whom PAI 1 activity increased progressively during the operation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In diabetic patients with microangiopathy significantly higher N acetyl beta glucosaminidase ( NAG ) activities in serum and E selection or ICAM 1 concentrations were found as compared with patients without microangiopathy , whereas plasma concentrations of tissue plasminogen activator ( tPA ) or inhibitor ( PAI 1 ) were comparable with those in the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Enzyme immunoassay ( ELISA ) was used to estimate the levels of plasminogen activators of urokinase ( uPA ) and tissue ( tPA ) types and one of their inhibitors ( PAI 1 ) in the cytosolic fraction of the thyroid in 129 patients with malignant and benign tumors and various non cancer diseases of the gland . ^^^ Tumors from patients with thyroid cancer displayed the lowest levels of tPA and the highest levels of uPA and PAI 1 , while those from patients with benign thyroid diseases , including adenoma , had high concentrations of tPA and relatively low levels of uPA and PAI 1 in the tissue of the diseased organ . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To establish the necessary and sufficient components of the PA system [ PAI 1 , urokinase type PA ( uPA ) , or tissue type PA ( tPA ) , and plasminogen ( Plg ) ] for angiogenesis , we examined angiogenic competence of vascular explant cultures obtained from mice deficient in PAI 1 , tPA , uPA , and Plg . ^^^ In summary , 1 ) PAI 1 by itself is a modest inhibitor of endothelial sprouting , 2 ) tPA and Plg are indispensable for angiogenesis in this model , 3 ) Plg is not the only substrate for PAs , and 4 ) the activity of MMP 9 is undetectable in explant cultures from tPA and Plg knockout mice . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antigen and activity levels of tPA and plasminogen activator inhibitor 1 ( PAI 1 ) in media from BEC infected with AdCMVtPA were analyzed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Michaelis complex , acylation , and conformational change steps were resolved in the reactions of the serpin , plasminogen activator inhibitor 1 ( PAI 1 ) , with tissue plasminogen activator ( tPA ) and trypsin by comparing the reactions of active and Ser 195 inactivated enzymes with site specific fluorescent labeled PAI 1 derivatives that report these events . ^^^ Anhydrotrypsin or S195A tPA induced fluorescence changes in P1 ' Cys and P 9 Cys PAI 1 variants labeled with the fluorophore , NBD , indicative of a substrate like interaction of the serpin reactive loop with the proteinase active site , with the P 1 ' label but not the P 9 label perturbing the interactions by 10 60 fold . ^^^ Only active trypsin and tPA induced the characteristic fluorescence changes in the labeled PAI 1 variants previously shown to report acylation and reactive loop sheet A interactions during the PAI 1 proteinase reaction . ^^^ By contrast , differing values of K ( M ) of 1 . 7 and 0 . 1 microM and of k ( lim ) of 17 and 2 . 6 s ( 1 ) for tPA reactions with P 1 ' and P 9 labeled PAI 1s , respectively , suggested that tPA PAI 1 exosite interactions , sensitive to the effects of labeling , promoted a rapid association of PAI 1 and tPA and reversible formation of an acyl enzyme complex but impeded a rate limiting burial of the reactive loop leading to trapping of the acyl enzyme complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) is a rapid inhibitor of tissue plasminogen activator ( tPA ) in circulation . ^^^ Evidence suggests that the PAI 1 concentration is responsible for the regulation of the endogenous fibrinolytic system through its tPA / PAI 1 interactions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS AND CONCLUSION : There was an increase of systemic levels of PAI 1 activity and tPA with progressive skin pathology in patients with CVI , and in the group with active ulcer there was an elevation of F 1 + 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Assays for F 1 + 2 , AT levels , TAT complex , APC activity , FPA , D Dimers , tPA , plasminogen , PAI 1 , and PAI 2 are more specific for fibrin formation and lysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelet aggregation in response to three doses of collagen and ADP , plasma concentrations of coagulation and fibrinolytic factors ( fibrinogen , factor 7 , tPA , PAI 1 ) and plasma concentrations of cell adhesion molecules ( soluble P selectin , E selectin , ICAM 1 , VCAM 1 ) were assessed twice , one week apart , at the end of each period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The components of the plasminogen activator system were measured , i . e . tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effects of low density lipoproteins ( LDL ) and lipoprotein ( a ) [ Lp ( a ) ] oxidized by O2 * / HO * free radicals generated by gamma radiolysis of water , on the release of tissue Plasminogen Activator ( tPA ) and of its main inhibitor Plaminogen Activator Inhibitor 1 ( PAI 1 ) by human umbilical vein endothelial cells ( HUVEC ) . ^^^ The results showed that O2 * / HO * free radical oxidized LDL and Lp ( a ) led to a dramatic decrease of PAI 1 release but did not affect tPA release , whereas copper oxidation of lipoproteins resulted in an increase in PAI 1 release and a decrease in tPA release . ^^^ Thus , the discordant effects of O2 * / HO * oxidized and copper oxidized LDL and Lp ( a ) on the endothelial releases of PAI 1 and tPA appeared to be due to qualitatively and / or quantitatively different formation of oxidized components by the two oxidation processes . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was undertaken to investigate the expression of the molecules of the PA system ( tPA , uPA , PAI 1 , uPAR , LRP ) , as well as several members of the MMP family and their inhibitors in the course of actively induced EAE in BALB / c mice . ^^^ Induction of expression of tPA and PAI 1 transcripts was detected in activated astrocytes in the white matter . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DRL performance in mice with deletion of tPA , uPA or PAI 1 genes . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of tPA and uPA activity , and is expressed in corresponding brain areas . ^^^ The current set of experiments were designed to investigate further the role of tPA and to extend our knowledge to uPA and PAI 1 , using mice with the respective genes deleted ( uPA / and PAI 1 / mice ) in the DRL 15 ' ' task . uPA / mice showed no disruption of DRL acquisition , but PAI 1 / mice showed a deficit similar to that seen in tPA / mice . ^^^ In an attempt to compensate for this deficit , experiments using a fixed number of reinforcers or a signalled DRL 15 ' ' schedule , similar to that used in rat lesion studies of DRL , were performed . tPA / mice were able to complete the signalled DRL task as well as their WTs , and , similarly , PAI 1 / mice were able to learn the fixed number of reinforcers DRL 15 ' ' schedule and the signalled DRL schedule . ^^^ These data indicate that uPA deletion does not affect performance of a standard DRL 15 ' ' task , whereas deletion of PAI 1 has the same behavioural consequences in these tasks as deletion of tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activation without changes in tPA and PAI 1 in response to subcutaneous administration of ancrod . ^^^ Plasmin generation is independent of an increase in tPA activity or changes in plasminogen activator inhibitor 1 ( PAI 1 ) concentration in plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunoprecipitation with a PAI 1 monoclonal antibody confirmed that both uPA and tPA were complexed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tPA and PAI 1 mRNA levels were estimated by reverse transcription polymerase chain reaction . ^^^ Chromogenic assay and enzyme linked immunosorbent assay were used to detect tPA activity and PAI 1 protein level in decidua . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To observe the effect of puerarin on superoxide dismutase ( SOD ) , lipid peroxidation ( LPO ) , tissue plasminogen ( TPA ) and plasminogen activator inhibitor ( PAI 1 ) of coronary heart disease ( CHD ) patients . ^^^ Nitrite colorimetric method , radioimmunoassay ( RIA ) , TBA fluorescent development process and fibrinolysin specific colorbase substrate decomposition development process were used to determine SOD activity , SOD , LPO , TPA and PAI 1 . ^^^ TPA and PAI 1 were increased significantly in the treated group , but was insignificantly changed in the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to inhibiting serine proteases , mainly tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators , PAI 1 interacts with different components of the extracellular matrix , i . e . fibrin , heparin ( Hep ) and vitronectin ( Vn ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Interleukin 1beta ( IL 1beta ) induced tPA and plasminogen activator inhibitor ( PAI ) 1 mRNA and supernatant tPA antigen were significantly inhibited by both NO donors , which resulted in a net decrease in the IL 1beta evoked tPA enzyme activity in the conditioned media . ^^^ Addition of the NO synthase inhibitor N monomethyl l arginine markedly increased the cytokine triggered tPA and PAI 1 mRNA levels , respectively . ^^^ Since MnTBAP , a superoxide dismutase mimetic , had no effects on the amplification of mRNA levels , we suggest that H2O2 is the candidate reactive oxygen species ( ROS ) responsible for the potentiation of IL 1beta triggered tPA and PAI 1 expression . ^^^ CONCLUSIONS : The temporal relationship between NO and ROS generation is a critical step in the modulation of tPA and PAI 1 expression in mesangial cells and may account for a dysregulation of matrix turnover during inflammatory processes in the renal mesangium . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous studies by our group indicated that nonenzymatically glycated low density lipoprotein ( LDL ) increased plasminogen activator inhibitor 1 ( PAI 1 ) production and decreased the generation of tissue plasminogen activator ( tPA ) from cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^ The present study demonstrates that plasma levels of PAI 1 or PAI 1 / tPA were significantly increased in patients with type 1 ( n = 10 ) and type 2 DM ( n = 14 ) compared with those in healthy controls ( n = 10 ; P < 0 . 05 or 0 . 01 ) . ^^^ LDL from patients with type 1 or type 2 DM , and very low density lipoprotein ( VLDL ) from patients with type 2 DM induced significantly greater increases in the release of PAI 1 and more profound reduction in tPA generation from HUVEC compared with corresponding lipoproteins from healthy controls ( P < 0 . 05 or 0 . 01 ) . ^^^ HDL from diabetic patients did not significantly alter the generation of PAI 1 or tPA from endothelial cells ( EC ) compared with HDL from controls . ^^^ Comparable effects of lipoproteins from DM patients on the generation of PAI 1 and tPA were found in human coronary artery EC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MEASUREMENTS AND RESULTS : After blood sampling we measured activation markers of fibrinolysis [ plasmin / alpha ( 2 ) antiplasmin complexes ( PAP ) , complexes of tissue plasminogen activator / plasminogen activator inhibitor ( tPA / PAI ) , fibrin ( ogen ) degradation products ( FDPs ) , D dimmers fibrin degradation products ( D d ) ] , the utilization marker of antithrombin 3 ( ATIII ) thrombin / antithrombin complexes ( TAT ) , several factors of fibrinolysis [ plasminogen , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , alpha ( 2 ) antiplasmin ] , and the natural coagulation inhibitors [ ATIII , protein C ( PrC ) , protein S ( PrS ) ] . ^^^ With the exception of a decrease in ATIII and an increase in tPA and PAI 1 , coagulation inhibitors and factors of fibrinolysis were not changed in sepsis . ^^^ In severe sepsis and mainly in septic shock , coagulation inhibitors ( ATIII , PrC ) and plasminogen were markedly decreased , whereas tPA and PAI 1 were further increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Patients with ACS had higher levels of tPA mass concentration , PAI 1 activity , D dimers mass concentration and TAT mass concentration at admission and on the 10th day as compared with those in SAP and normal subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This activity was inhibited by plasminogen activator inhibitor 1 or antibodies to uPA but not tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the association between haemostatic parameters and increased risk of myocardial infarction ( MI ) at a young age , we measured fibrinogen , factor 7 , antithrombin 3 , protein C , protein S , tissue factor ( TF ) , free form tissue factor pathway inhibitor ( TFPI ) , plasminogen , alpha 2 antiplasmin , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and lipoprotein ( a ) in 140 young men with MI before age 45 and 150 age matched healthy men . ^^^ TF , TF / TFPI ratio , PAI 1 , PAI I / tPA ratio , plasminogen , and lipoprotein ( a ) in young MI patients were all significantly higher than controls , while TFPI , antithrombin 2 , and tPA were significantly lower ( P < 0 . 001 of each ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin formed from plasma collected during and immediately post CABG was associated with increased fibrinolytic capacity and enhanced binding of a ) MMP 9 , b ) the low Mw protease triplet ( described above ) , and c ) PA ( as putative 110 kDa tPA : PAI 1 complex ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Statins downregulate TF mitigating thrombin generation and also downregulate PAI 1 , which normally consumes tissue plasminogen activator ( tPA ) . ^^^ In the absence of PAI 1 , tPA activates plasminogen to generate plasmin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIMS : To study long term effects of enalapril on mass concentrations of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) , tPA / PAI 1 complex and von Willebrand factor ( vWF ) in both genders with uncomplicated myocardial infarction . ^^^ PAI 1 , tPA , tPA / PAI 1 complex and vWF were measured after two weeks , six and 12 months following randomisation . ^^^ The tPA / PAI 1 complex decreased and was significantly lower ( mean reduction 0 . 96 microg 10 L ( 1 ) , CI : 0 . 36 to 1 . 56 microg 10 L ( 1 ) , p = 0 . 003 ) in the enalapril group after two weeks and six months ( p = 0 . 037 ) . ^^^ CONCLUSIONS : Enalapril treatment up to one year depressed mass concentrations of PAI 1 and transiently tPA and tPA / PAI 1 complex indicating an improvement of the fibrinolytic balance in both genders with uncomplicated myocardial infarction . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The overall normal vessel architecture and the antigenicity of von Willebrand factor , tPA and plasminogen activator inhibitor type 1 ( PAI 1 ) were retained for at least 9 days . tPA was constantly secreted into the conditioned medium at least up to day 12 . ^^^ C ) on the release of tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Analysis of plasminogen activator inhibitor 1 ( PAI 1 ) expression showed that PAI 1 antigen is upregulated by 24 hr and could account for the tPA activity downregulation seen at this time point . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and urokinase ( uPA ) are targets of plasminogen activator inhibitor 1 ( PAI 1 ) inhibition . ^^^ Activation of protein kinase A ( PKA ) , however , lowered PAI 1 secretion induced by uPA and tPA , as a result of an inhibition of the PKC pathway and inhibition of Raf , Mek and MAPK phosphorylations . ^^^ These multiple pathways utilized by uPA and tPA to modulate PAI 1 secretion might be involved in determining the proteolytic or antiproteolytic potential of the SMCs under different pathophysiological conditions . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) are the major regulators of plasmin generation . ^^^ Glomerular PAI 1 / tPA balance is involved in extracellular matrix turnover , as well as fibrin deposition in glomeruli . ^^^ We quantified glomerular PAI 1 and tPA messenger RNA ( mRNA ) by competitive polymerase chain reaction . ^^^ There was a sixfold increase in PAI 1 tPA mRNA ratio in patients with MN compared with the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The serine proteinase inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) is the primary physiological inhibitor of the tissue type and the urokinase type plasminogen activator ( tPA and uPA , respectively ) and as such an important regulator of proteolytic events taking place in the circulation and in the extracellular matrix . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cytosol of primary breast cancers from 217 women of predominantly Arab ethnicity were assayed for uPA , tPA , PAI 1 and a subset for ER , PR and pS 2 . ^^^ Only tPA correlated to nodal status and tumour grade , and PAI 1 to clinical stage . ^^^ Women with high tumour uPA and PAI 1 , but not tPA , had shorter overall , and relapse free , survival . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma levels of von Willebrand factor , tissue plasminogen activator ( TPA ) , plasminogen activator inhibitor ( PAI 1 ) , and the TPA PAI 1 complex were significantly increased in TTP / HUS patients ; however , the levels of these markers were not significantly different between TTP / HUS patients who survived and those who died , suggesting that these markers might not be directly related to outcome . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hemostatic factors of endothelial origin , i . e . , plasminogen activator inhibitor 1 ( PAI 1 mass ) , von Willebrand Factor ( vWF ) , and D dimer were analyzed by ELISA , and tissue plasminogen activator ( tPA ) activity was analyzed by a chromogen method . ^^^ ESR , IL 2sR alpha , PAI 1 , tPA activity , vWF , D dimer , homocysteine , aCL ( IgA ) , and MDA LDL ab ( IgA ) correlated with sICAM 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fasting blood samples were collected before and after 12 month treatment for determinations of fibrinogen , prothrombin fragment 1+2 ( F1+2 ) , plasma D dimer , soluble tissue factor , tissue plasminogen activator ( tPA ) antigen , tPA activity , plasminogen activator inhibitor type 1 activity ( PAI 1 activity ) and serum D dimer as a global test of fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the consequences of the gene polymorphisms of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in patients positive for aPL . ^^^ METHODS : Seventy seven Japanese and 82 British patients with aPL were examined for Alu repeat insertion ( 1 ) / deletion ( D ) polymorphism of the tPA gene by polymerase chain reaction ( PCR ) , and 4G / 5G polymorphism in the PAI 1 promoter gene by site directed mutagenesis PCR and restriction fragment length polymorphism analysis . ^^^ CONCLUSION : Polymorphisms of the tPA or PAI 1 genes probably do not significantly influence the risk of anerial thrombosis , venous thrombosis , or pregnancy morbidity in patients with aPL . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , serum levels of plasminogen activator inhibitor 1 ( PAI 1 ) , thrombomodulin ( TM ) , tissue plasminogen activator ( tPA ) , and tissue factor ( TF ) were determined . ^^^ Furthermore , transient increases in PAI 1 , increases in TM , and increases in tPA were observed in the serum of some but not all recipients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Resting plasma von Willebrand factor antigen ( vWF Ag ) and tissue plasminogen activator ( tPA ) antigen concentrations and plasminogen activator inhibitor 1 ( PAI 1 ) activity were higher in the patients compared with control subjects . ^^^ Both tPA antigen concentration and activity increased and PAI 1 activity decreased significantly with exercise in both groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
ApoE genotype does not affect plasma tPA and PAI 1 antigen levels . ^^^ One receptor for the ApoE protein is the LRP / alpha 2 macroglobulin receptor , which also binds to and endocytoses tPA and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ We demonstrate that there is no difference in plasma levels of tPA and PAI 1 antigens between middled aged individuals with one apoE 4 allele and those having none . ^^^ This suggests that the impact of apoE 4 on Alzheimer ' s disease is not the result of altered clearance of tPA or PAI 1 by the LRP receptor . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After 48hr mRNA expression of MMP 1 , MMP 3 , TIMP 1 , uPA , tPA and PAI 1 was analyzed by RT PCR ELISA . ^^^ Both drugs inhibited the IL 1 induced mRNA expression of tPA , whereas expression of uPA was only mildly reduced by PSGAG , which also induced PAI 1 above IL 1 stimulated levels . ^^^ In this study we determined the in vitro effects of polysulfated glycosaminoglycan ( PSGAG ) and the glucocorticoid triamcinolone acetonid ( TA ) on the IL 1 altered expression and activity of matrix metalloproteinases ( MMP 1 , MMP 3 ) , tissue inhibitor of metalloproteinases 1 , the plasminogen activators tPA and uPA and plasminogen activator inhibitor 1 by articular chondrocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TCDD induced the early appearance of ovarian plasminogen activator inhibitor type 1 ( PAI 1 ) , plasminogen activator inhibitor type 2 ( PAI 2 ) , urokinase plasminogen activator ( uPA ) , and tissue plasminogen activator ( tPA ) at 24h after dosing when compared with controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
One hundred and six subjects with peripheral arterial disease ( PAD ) and intermittent claudication were characterized on the activity level of tissue plasminogen activator ( tPA , the activator of fibrinolysis ) , the activity level of plasminogen activator inhibitor ( PAI 1 , the inhibitor of fibrinolysis ) , daily physical activity , ambulatory function , and demographic information . ^^^ Group differences in tPA activity and PAI 1 activity persisted after our controlling for group differences in measured and self reported ambulatory measures . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , dexamethasone increased the steady state mRNA level of the tPA inhibitor 1 ( PAI 1 ) , thereby providing an additional mode of regulation of tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effects of low density lipoprotein ( LDL ) on PA inhibitor 1 ( PAI 1 ) , urokinase type PA ( uPA ) , and tissue type PA ( tPA ) in relationship to protein kinase C ( PKC ) in cultured human mesangial cells ( HMC ) . ^^^ LDL downregulated 2 . 4 kb PAI 1 , uPA , and tPA mRNA expression within 6 h of incubation with HMC . ^^^ On the other hand , after 12 48 h , LDL treated cells showed a significant increase in PAI 1 , tPA , and uPA mRNA levels . ^^^ The stimulatory effects of LDL on PAI 1 , tPA , and uPA gene regulation in HMC were blocked by the inhibition of PKC using GF 109203X 12 h after treatment with LDL or downregulation of PKC using phorbol myristate acetate . ^^^ In summary , LDL regulates PAI 1 , uPA , and tPA in biphasic patterns in HMC , and the upregulation of PAI 1 , uPA , and tPA after long term LDL exposure seems to be mediated by a delayed PKC activation associated with an increased PA inhibitory activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to aspartyl and cysteineproteinases , serine proteinases including the plasminogen activator system ( uPA , uPAR , tPA , PAI 1 and PAI 2 ) and matrix metalloproteinases ( MMPs ) with their tissue inhibitors ( TIMPs ) play an essential role in these processes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we describe an assay for the assessment of components of the plasminogen activation system ( uPA , tPA and PAI 1 , and their complexes ) in blood which is not susceptible to interference by heterophilic antibodies . ^^^ The assay is compared to our earlier reported ELISA for measuring uPA , tPA and PAI 1 components in tumor tissue extracts . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Low levels of tPA or excessive levels of plasminogen activator inhibitor 1 ( PAI 1 ) cause hypofibrinolysis , causally related to the development of atherosclerosis and associated thrombotic complications , as well as with the development of venous and arterial thrombosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We evaluated the concentration and activities of tPA , uPA and PAI 1 in plasma from kidney allograft recipients . ^^^ In kidney transplant recipients we found significantly higher plasma tPA activity ( median : 0 . 99 IU / ml ; range : 0 3 . 8 IU / ml ) in comparison to healthy controls ( median : 0 . 15 IU / ml ; range : 0 2 . 8 IU / ml ) ( p = 0 . 002 ) as well as significantly lower plasma PAI 1 activity ( median : 7 . 06 U / ml ; range : 0 33 . 2 U / ml ) in comparison to healthy controls ( median : 21 . 8 U / ml ; range : 0 36 . 7 U / ml ) , ( p = 0 . 0001 ) . ^^^ In kidney transplant recipients there was a positive correlation between the dose of prednisone and PAI 1 activity in plasma ( p = 0 . 01 ) and an association between BMI value and plasma PAI 1 activity ( p = 0 . 008 ) , as well as an association between BMI value and plasma tPA Ant concentration ( p = 0 . 006 ) . ^^^ Renal allograft recipients have higher tPA and lower PAI 1 activities in plasma in comparison to healthy individuals . ^^^ In kidney graft recipients there is a relation between the value of BMI and the activity and concentration of tPA Ant as well as the value of BMI and the PAI 1 activity in plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have delineated two different reaction mechanisms of monoclonal antibodies ( mAbs ) , MA 8H9D4 and either MA 55F4C12 or MA 33H1F7 , that convert plasminogen activator inhibitor 1 ( PAI 1 ) to a substrate for tissue ( tPA ) and urokinase plasminogen activators . ^^^ MA 55F4C12 and MA 33H1F7 ( 50 80 % substrate reaction ) do not interfere with initial PAI 1 / proteinase complex formation but retard the inhibitory pathway by decreasing k ( lim ) ( > 10 fold for tPA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the in vitro effects of several nonsteroidal antiinflammatory drugs on the IL 1 altered expression and activity of tPA , uPA and PAI 1 by articular chondrocytes . ^^^ Expression of mRNA for the plasminogen activators ( uPA and tPA ) and their inhibitor ( PAI 1 ) were analyzed by RT PCR ELISA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The fibrinolytic activities of conditioned medium and cell lysates from human glioma cell lines , A 172 , T98G , U 87 and TM 1 were studied by fibrin plate zymography . mRNA expression of tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) was measured by Northern blot analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentration of suPAR was significantly correlated to plasma lactate dehydrogenase , thrombomodulin , and complex of tPA : PAI 1 in the patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Diurnal decreases in tPA and tPA PAI ranged from 1 . 5 ( VE ) to 1 . 3 ( controls ) , whereas the diurnal decrease in PAI 1 was more than fourfold in VE and 2 . 7 fold in controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood levels of plasminogen , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) , were measured before and after stress ( 5 minutes of stair climbing ) in a group of 30 women , 23 40 years old , who had taken 30 mcg of ethinyl estradiol with 150 mcg of desogestrel or levonorgestrel for at least 1 year . ^^^ Levels of tPA and PAI , although slightly increased in the oral contraceptive group , were not significantly different between the two groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activities and concentrations of tissue plasminogen activator ( tPA ) , plasminogen activator ( PAI ) type 1 , and tPA / PAI complex were determined by ELISA kits . ^^^ Concentrations and activities of tPA , PAI 1 , and tPA / PAI complex did not differ between the groups at any time . ^^^ CONCLUSION : Peritoneal concentrations and activities of tPA , PAI 1 , and tPA / PAI complex are similar during laparoscopic and conventional colorectal resections . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activities and concentrations of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tPA / PAI complex , fibrinogen , and D dimers were determined in all specimen with ELISA tests . ^^^ Postoperatively , tPA activity decreased significantly in both groups , but AUC values for tPA and PAI 1 activity ( p = 0 . 23 ; p = 0 . 68 ) ; concentration of tPA , PAI 1 , and tPA / PAI complex ( p = 0 . 52 ; p = 0 . 78 ; p = 0 . 95 ) ; and concentration of fibrinogen and D dimers ( p = 0 . 67 ; p = 0 . 71 ) did not differ between the groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Factor VIIc , antithrombin , fibrinogen , thrombin antithrombin complex ( TAT ) , FDP ( D Dimer ) , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured in 80 healthy postmenopausal women after 3 , 6 and 12 months therapy with either 17beta oestradiol / norethisterone acetate or tibolone . ^^^ RESULTS : Both treatments significantly reduced fibrinogen , factor VIIc , antithrombin , tPA and PAI 1 antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Leukocyte count was significantly correlated to body mass index , waist to hip ratio , blood pressure , plasma triglycerides , high density lipoprotein cholesterol ( inversely ) , fasting and postprandial plasma glucose , insulin and proinsulin , PAI ( active ) , tPA and microalbuminuria , as well as to smoking and physical activity ( inversely ) . ^^^ Fibrinogen level was significantly correlated with body mass index , systolic blood pressure , plasma triglycerides , fasting plasma glucose , HbA1c , PAI ( active ) , tPA and von Willebrandt factor , as well as with smoking and low physical activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : It has been proposed that the mechanism of action of the new risk factors for myocardial infarction and stroke , von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) and tissue plasminogen activator inhibitor 1 ( PAI 1 ) could possibly be mediated via a primary effect on atherogenesis but there is little data to substantiate this . ^^^ There were small positive associations of plaque area with vWF , tPA and tPA / PAI 1 complex and a tendency to negative associations with PAI 1 levels , independent from the traditional risk factors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Median activity of the plasminogen activator inhibitor 1 ( PAI 1 ) and the concentration of TPA PAI 1 complexes were increased to approximately double those in nonsevere haemophiliacs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To test the hypothesis that during wounding , exposed collagen , the most abundant ECM molecule in the skin , regulates keratinocyte PA and PAI gene expression , we utilized an in vitro model in which activated keratinocytes were cultured in dishes coated with collagen or other ECM substrates . tPA , uPA , and PAI 1 mRNA and enzymatic activity were detected when activated keratinocytes attached to fibronectin , vitronectin , collagen 4 , and RGD peptide . ^^^ In contrast , adhesion to collagen 1 and collagen 3 completely suppressed expression of PAI 1 mRNA and protein and further increased tPA expression and activity . ^^^ Similarly , keratinocyte adhesion to laminin 1 suppressed PAI 1 mRNA and protein expression and increased tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis was quantified with measurement of the activities of tissue plasminogen activator ( tPA ; the activator of fibrinolysis ) and its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) with amidolytic technique . ^^^ RESULTS : tPA activity in all groups was normal at baseline ; baseline PAI 1 activity was elevated . ^^^ Within each prophylaxis group , no significant changes occurred in either tPA or PAI 1 activities after induction of anesthesia or after surgery compared with before surgery ( P > . 05 , analysis of variance with repeated measures ) . ^^^ No significant enhancement of fibrinolysis , manifested as either increased tPA activity or decreased PAI 1 activity , occurred in either EPC group compared with the HEP group at any time point ( P > . 05 , analysis of variance with repeated measures ) . ^^^ CONCLUSION : In this study , enhanced regional fibrinolysis in the lower extremities could not be detected with the use of EPCs , as measured with tPA and PAI 1 activity in common femoral venous blood samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An increase in homocysteine level was associated with a rise in plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( TPA ) antigen , von Willebrand factor and fibrinogen level . ^^^ After regression analyses adjusting for covariates , there remained significant associations between homocysteine and PAI 1 and TPA antigen . ^^^ CONCLUSION : Increasing homocysteine levels are associated with impaired fibrinolytic potential , as indicated by increased PAI 1 and TPA antigen levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DNA synthesis was assessed by measuring the incorporation of [ 3H ] thymidine into cellular DNA fraction . tPA , uPA and type 1 plasminogen activator inhibitor ( PAI 1 ) gene expressions were measured by Northern blotting . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The serpin plasminogen activator inhibitor type 1 ( PAI 1 ) , as the primary physiological inhibitor of both urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator , plays an important role in the regulation of the fibrinolytic system as well as in extracellular remodeling in both physiological and pathophysiological processes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate the relationship between coagulation activities and the fibrinolytic system during normal pregnancy , we measured the plasma concentrations of coagulation factors , antithrombin 3 ( AT 3 ) , D dimer , tissue plasminogen activator ( tPA ) , total protein S ( TPS ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in 436 apparently healthy pregnant , postpartum , and nonpregnant women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum components , such as lipoproteins , coagulation factors ( factor 7 , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , fibrinogen ) , and homocysteine have been associated with cardiovascular disease . ^^^ However , there was a slight favorable trend for tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A positive correlation between area under insulin curve and PAI 1 activity and a negative correlation between fasting insulin levels and tPA were also observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Collagen and PAF stimulated platelet aggregation , collagen stimulated thromboxane release ( TXB 2 ) , plasma tPA and PAI 1 antigens , von Willebrand factor , p selectin , and flow mediated and glyceryl trinitrate mediated dilatation of the brachial artery , were examined before and at the end of intervention . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , fibrinolytic factors ( plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) and fibrinogen ) were measured in patients who received fibrinolysis . ^^^ This reduction was associated with elevation of PAI 1 activity and reduction of tPA activity , independent of changes in plasma insulin levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At each time point , six coagulation parameters ( platelet count , fibrinogen level , factor 7 activity , thrombin antithrombin complex [ TAT ] level , tissue plasminogen activator [ tPA ] activity , and plasminogen activator inhibitor [ PAI ] activity ) were measured as well as all standard blood count parameters . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To determine whether normal peritoneal and adhesion fibroblasts express tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor ( PAI 1 ) and whether their expression is regulated by oxygen . ^^^ MAIN OUTCOME MEASURE ( S ) : We have used the multiplex reverse transcription polymerase chain reaction ( RT PCR ) technique to determine the effect of hypoxia on the expression of tPA and PAI 1 in normal peritoneal ( NPF ) and adhesion ( ADF ) fibroblasts . ^^^ RNA was extracted from cells and subjected to multiplex RT PCR to quantitate relative changes in mRNA levels of tPA and PAI 1 in response to hypoxia treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here we found that the serine protease tissue plasminogen activator ( tPA ) was upregulated in the central and medial amygdala by acute restraint stress , where it promoted stress related neuronal remodeling and was subsequently inhibited by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We observed a significant difference between diabetic patients and controls as regards tPA ( 11 . 8 + / 5 . 4 vs . 6 . 6 + / 3 . 0 ng / ml ; p < 0 . 05 ) and PAI 1 Act ( 17 . 8 + / 9 . 2 vs . 11 . 7 + / 6 . 6 ng / dl ; p < 0 . 005 ) . ^^^ After 4 years 13 diabetic patients became vasculopathic and , at baseline , had significantly lower tPA ( 8 . 9 + / 4 . 8 vs . 12 . 5 + / 5 . 3 ; p < 0 . 011 ) , and higher PAI 1 Ag ( 50 . 8 + / 22 . 2 vs . 32 + / 22 . 2 ; p < 0 . 006 ) , and PAI 1 Act values ( 24 . 1 + / 9 . 5 vs . 16 . 1 + / 8 . 4 ; p < 0 . 014 ) , compared with 50 diabetic patients who did not develop PAD after 4 years . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fasting blood samples for determination of tPA and plasminogen activator inhibitor ( PAI 1 ) enzyme activities were obtained before , immediately after , and 60 min after submaximal treadmill walking . ^^^ RESULTS : The exercise bout increased tPA activity by 79 % ( P < 0 . 01 ) and decreased PAI 1 activity by 18 % ( P < 0 . 01 ) . ^^^ At 1 h after completing the walking exercise , plasma tPA activity levels were still significantly elevated ( 43 % , P < 0 . 01 ) , and PAI 1 activity levels were 25 % lower ( P < 0 . 01 ) than baseline . ^^^ Significant increases in endogenous tPA and reductions in PAI 1 activity persist for at least 1 h after exercise cessation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : In CHD patients , FINS , TC , TG , LDL C and PAI 1 levels were higher and ISI , HDL C and tPA before and during VOT were lower than those in the healthy controls . ^^^ After puerarin treatment , FINS level lowered and ISI increased significantly ( P < 0 . 01 ) , while comparing with the routine group , TC , TG , LDL C and PAI 1 were lower but HDL C and tPA activity before and during VOT were higher in the puerarin group ( P < 0 . 05 , P < 0 . 01 ) . ^^^ Correlation analysis showed that FINS was positively correlated with TC , TG , LDL C and PAI 1 and negatively correlated with HDL C , tPA before and during VOT ; ISI was negatively correlated with TC , TG , LDL C and PAI 1 and positively correlated with HDL C and tPA before and during VOT in the puerarin group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , antioxidants failed to affect tPA and PAI 1 secretion , which was increased by LDL , either native or oxidized . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to these metabolic changes there are also other laboratory deviations , manifested e . g . by hyperfibrinogenaemia and an increased concentration of tPA inhibitor ( PAI 1 ) without detectable endothelial dysfunction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before HD , the plasma levels of fibrinogen , plasmin plasmin inhibitor complex ( PIC ) , thrombomodulin ( TM ) , and D dimer were increased , while the plasma levels of protein C ( PC ) , antithrombin ( AT ) , thrombin antithrombin complex ( TAT ) , and tissue plasminogen activator ( tPA ) plasminogen activator inhibitor 1 ( PAI 1 ) complex ( tPA PAI 1 complex ) were decreased . ^^^ The plasma levels of TAT and TPA PAI 1 complex were significantly increased and those of PIC , soluble fibrin ( SF ) and D dimer tended to be high in patients with less than 0 . 7 of ABI . ^^^ The plasma levels of D dimer , TPA PAI 1 complex , TAT , PIC , and SF tended to be high in patients with more than 0 . 5 in ABI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the efficacy of 4 different fibrinolysis system parameters , namely , tissue plasminogen activator ( tPA ) , tissue plasminogen activator inhibitor type 1 ( PAI 1 ) , plasmin antiplasmin complexes ( PAP ) , and D dimer , in the diagnosis of acute PE . ^^^ MAIN OUTCOME MEASURES : At the moment of clinical suspicion , a sample of venous blood was obtained to measure levels of tPA , PAI 1 , PAP , and D dimer using an enzyme linked immunosorbent assay method . ^^^ The sensitivity / negative predictive value for tPA , using a cutoff of 8 . 5 ng / mL , and PAI 1 , using a cutoff of 15 ng / mL , were 100 % / 100 % and 100 % / 100 % , respectively . ^^^ A tPA level lower than 8 . 5 ng / mL occurred in 13 ( 19 . 7 % ; all PE negative ) of 66 patients with suspected PE , and PAI 1 levels were lower than 15 ng / mL in 9 ( 13 . 6 % ; all PE negative ) of 66 patients with suspected PE . ^^^ CONCLUSIONS : Our data indicate that tPA and PAI 1 levels are potentially useful in ruling out PE , although tPA seems to be the better parameter . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of orosomukoid , C reactive protein , prealbumin , transferin , alpha 2 macroglobulin , PAI 1 , tPA , sE Selectin , SICAM , sP Selectin , antithrombin 3 , fibrinogen , haemoglobin , time of Quick test , TT , APTT , number of leukocytes , erythrocytes and thrombocytes were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , renal expression of several genes that regulate plasmin activity were similar in both genotypes , including uPA , tPA , PAI 1 , protease nexin 1 , and alpha 2 antiplasmin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Multivariate genetic analysis was performed using a total of 314 ( 107 monozygotic and 207 dizygotic ) twin pairs on IR assessed by HOMA , fibrinogen , plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( tPA ) , factor 8 ( FVIII ) , von Willebrand factor ( vWF ) and factor 13 B subunit . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of lipids , lipoproteins , acute phase reactants , tHcy and atherothrombotic markers [ fibronectin , soluble vascular adhesion molecules 1 ( sVCAM 1 ) , soluble intercellular adhesion molecules 1 ( sICAM 1 ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , autoantibodies against oxidized LDL ( AuAb oxLDL ) ] were determined . ^^^ RESULTS : The mean levels of serum tHcy , fibrinogen , fibronectin , sICAM , PAI 1 and AuAb oxLDL were increased in patients whereas tPA , vitamin B ( 12 ) and folate levels were decreased significantly . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( serpin ) superfamily , is the principal inhibitor of tPA and uPA in the fibrinolytic system . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( serpin ) superfamily , is the principal inhibitor of tPA and uPA in the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study we investigated the effect of combined antioxidant treatment with vitamins C and E on endothelial function and plasma levels of plasminogen activator inhibitor ( PAI 1 ) , von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) and factor 7 ( fVII ) , in smokers . ^^^ Plasma levels of PAI 1 and vWF were decreased only in group C ( p < 0 . 05 for both ) , while PAI 1 / tPA ratio was significantly decreased in both groups B and C ( p < 0 . 05 for both ) . ^^^ In conclusion , combined administration of vitamin C and vitamin E at high dosages , improved endothelial function and decreased plasma levels of PAI 1 , vWF and PAI 1 / tPA ratio in chronic smokers . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators and their type 1 inhibitor ( PAI 1 ) were determined by immunoassay in tumor cytosols and samples of histologically unaltered adjacent mucosa in gastric cancer patients . ^^^ Gastric tumor revealed enhanced uPA and PAI 1 matched by decreased tPA in intact mucosa . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since endothelial cells play a pivotal role in the regulation of hemostasis , we studied the effect of DV infection on the production of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in vitro using both primary isolated endothelial cells , human umbilical cord veins cells , and a human microvascular endothelial cell line . ^^^ DV infection significantly induced the secretion of tPA but not PAI 1 of human endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Analysis of tissue samples on reducing and non reducing polyacrylamide gels demonstrates complexes of tPA with plasminogen activator inhibitor 1 ( PAI 1 ) but not with neuroserpin , a tPA specific inhibitor concentrated in grey matter . ^^^ As total tPA protein remains unchanged in acute lesions and the concentration of PAI 1 rises several fold , complex formation is a probable cause of the impaired fibrinolysis . ^^^ The impaired fibrinolytic capacity resulting from increased PAI 1 synthesis and complex formation with tPA , which is detectable prior to lesion formation , therefore has the potential to contribute to axonal damage in multiple sclerosis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to investigate whether epidermal growth factor receptor ( EGF R ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) are involved in this process . ^^^ The concentrations of serum EGF R , plasma tPA , PAI 1 , and PAI 2 , and the strength of wound healing were measured . ^^^ CONCLUSIONS : In the rats with octreotide irrigation , the EGF R level was decreased , the plasma tPA concentration was higher , and the plasma PAI 1 and PAI 2 concentrations were lower when compared with values in group C and group NS rats on days 7 and 14 after surgery . ^^^ The data suggest that EGF R , tPA , PAI 1 , and PAI 2 are all involved in the mechanism of octreotide ' s action in reducing adhesion formation . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At the end of the placebo run in period and of each treatment period , BP , plasma PAI 1 and tPA activity were evaluated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular endothelial cells ( EC ) synthesize activators and inhibitors for fibrinolysis , tissue and urokinase plasminogen activators ( tPA and uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Increased levels of PAI 1 with various levels of tPA have been frequently found in plasma of patients with coronary heart disease ( CHD ) or diabetes mellitus ( DM ) . ^^^ Oxidative modification by copper , ultraviolet or long exposure to EC enhanced the effect of LDL on the generation of PAI 1 and tPA from EC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We demonstrated that luteal cells from the rhesus monkey were able to produce both tissue type PA ( tPA ) and urokinase type PA , as well as the physiological PAI 1 . ^^^ This change of tPA levels was in a temporal coordination with the regulation of PAI 1 expression , resulting in an increased tPA activity at the initiation of luteolysis . ^^^ A PAI 1 modulated tPA activity might be important for the initiation of luteolysis in the monkey . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of tissue plasminogen activator ( TPA ) antigen , D dimer , and activity of plasminogen activator inhibitor ( PAI ) were measured before and in 24 hours , 3 , 7 , 14 days after randomization . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Stepwise regression analysis , with established predictors of plasma tPA including plasma PAI 1 and genetic variants , showed that neither genotypes nor haplotypes were major contributors to plasma tPA . ^^^ In conclusion , in the present study neither genetic variation at the PLAT locus nor the PAI 1 675 4G > 5G polymorphism was strong predictors of plasma tPA levels , which suggests that variations in other genes contribute to the heritability of this phenotype . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The study group consisted of 31 patients ( 17 males , mean age 62 years , and 14 females , mean age 60 . 6 years ) with ACS and without ST segment elevation in whom antibodies to C . pneumoniae and such haemostatic factors as von Willebrand factor ( vWF ) , thrombomodulin ( TM ) , tissue plasmin activator ( tPA ) , tPA inhibitor ( PAI 1 ) and fibrinogen were measured . ^^^ No significant relationship between vWF , TM , tPA and PAI 1 levels , and C . pneumoniae infection was found whereas a significant ( p=0 . 05 ) relationship between C . pneumoniae specific IgG antibodies and fibrinogen level was detected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
High plasma levels or activities of fibrinogen , factor 7 , factor 8 , von Willebrand factor ( vWF ) , soluble thrombomodulin , tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) are thought to be associated with increased morbidity and mortality related to cardiovascular disease . ^^^ By altering the levels and activities of tPA and PAI 1 , statins seem to stimulate fibrinolysis . ^^^ In this way they probably regulate the synthesis of TF , tPA and PAI 1 , and perhaps they also control the generation and activity of thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The allograft microenvironment was examined using immunohistochemistry for fibrogenic markers ( transforming growth factor [ TGF ] beta , platelet derived growth factor [ PDGF ] , tissue plasminogen activator [ TPA ] , plasminogen activator inhibitor [ PAI ] 1 , matrix metalloproteinase [ MMP ] 2 , and tissue inhibitor of matrix metalloproteinase [ TIMP ] 2 ) . ^^^ Immunostaining for TGF beta , PDGF , TPA , PAI 1 , MMP 2 and TIMP 2 was observed in all allografts at the time of rejection . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We analysed three polymorphisms located at the BACE 1 gene , one polymorphism at the ACHE gene , and two variants located at the tissue plasminogen activator and plasminogen activator inhibitor 1 ( genes TPA and PAI 1 , respectively ) , both part of the plasmin system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
While tissue plasminogen activator ( tPA ) and adipsin , an adipocyte derived protease , were unaffected by LIP , changes in soluble vascular cell adhesion molecule 1 ( sVCAM 1 ) were significantly correlated ( p = 0 . 02 ) with those seen for PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The study group consisted of 31 patients ( 17 males aged 38 78 years and 14 females aged 45 80 years ) with ACS and without ST segment elevation in whom we measured antibodies to H . pylori and haemostatic factors indicating endothelial function , such as von Willebrand factor ( vWF ) , thrombomodulin ( TM ) , tissue plasmin activator ( tPA : Ag ) , tPA inhibitor ( PAI 1 : Ag ) and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hyperthyroid ( n=10 ) , hypothyroid ( n=10 ) and subclinical hypothyroid ( n=10 ) patients and control cases ( n=16 ) were evaluated for free and total tissue factor pathway inhibitor ( tTFPI ) , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) levels in disease and euthyroid states . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In vitro and in vivo effects of tPA and PAI 1 on blood vessel tone . ^^^ The 2 putative vasoactive epitopes of tPA are regulated by the plasminogen activator inhibitor 1 ( PAI 1 ) and by a PAI 1 derived hexapeptide that binds tPA . ^^^ TNK tPA , a tPA variant in which the PAI 1 docking site has been mutated , stimulates PE induced vasoconstriction at all concentrations used . ^^^ Our findings draw attention to a novel role of tPA and PAI 1 in the regulation of blood vessel tone that may affect the course of ischemic diseases . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We determined the changes in tPA and plasminogen activator inhibitor ( PAI 1 ) expression in adult rat brain after transient middle cerebral artery ( MCA ) occlusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the effect of vitamin C on forearm vasodilatory response to reactive hyperemia and on plasma level of plasminogen activator inhibitor 1 ( PAI 1 ) , von Willebrand factor ( vWF ) , tissue plasminogen activator ( tPA ) , antithrombin 3 ( ATIII ) , proteins C and S , and factors 5 ( fV ) and 7 ( fVII ) in patients with both type 2 diabetes and CAD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The plasma levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured before and after treatment in the captopril group ( group A , 35 cases ) and the routine group ( group B , 37 cases ) of patients with HAPE , while 20 healthy volunteers served as the control ( group C ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In two different mouse models of Alzheimer ' s disease , chronically elevated Abeta peptide in the brain correlates with the upregulation of plasminogen activator inhibitor 1 ( PAI 1 ) and inhibition of the tPA plasmin system . ^^^ In addition , Abeta injected into the hippocampus of mice lacking either tPA or plasminogen persists , inducing PAI 1 expression and causing activation of microglial cells and neuronal damage . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood was collected immediately before and after each test and analyzed for von Willebrand Factor antigen ( vWF : ag ) , tissue plasminogen activator ( tPA ) antigen , and plasminogen activator inhibitor 1 ( PAI 1 ) activity . ^^^ TM resulted in significant increases in tPA antigen ( 6 . 54 + / 2 . 76 ng . mL 1 preexercise , 21 . 39 + / 10 . 56 ng . mL 1 postexercise ) and both SS and TM caused significant reductions in PAI 1 activity ( SS = 15 . 1 + / 3 . 8 AU . mL 1 preexercise , 13 . 2 + / 4 . 3 AU . mL 1 postexercise ; TM = 15 . 3 + / 3 . 6 AU . mL 1 preexercise , 10 . 5 + / 5 . 3 AU . mL 1 postexercise ) . ^^^ Postexercise PAI 1 activity was significantly lower for TM versus SS . tPA antigen was unchanged after SS and ST , and PAI 1 activity was unchanged after ST . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study utilized mesangial cells isolated from mice with gene deletions for tPA , uPA , and plasminogen activator inhibitor 1 ( PAI 1 ) to further delineate the role of the PA / plasminogen / plasmin system in ECM accumulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In CEC , captopril also upregulated tPA and its inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of D dimer ( DD ) , thrombin antithrombin complex ( TAT ) , prothrombin fragment 1+2 ( F1+2 ) , von Willebrand factor ( vWF ) , fibrinogen , tissue plasminogen activator ( TPA ) and activity of its inhibitor ( PAI ) , soon after start of treatment with heparins and in 1 , 3 , 7 and 14 days . ^^^ In LMWH treated patients these changes ( elevations of TPA level ) became manifested early ( on day 3 ) and were short lived while in UFH treated patients they appeared later ( on day 7 ) and were prolonged ( as lowered PAI activity on day 14 ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present review the dates about association of genetic variants of a plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) with ischemic heart disease and atherosclerosis of other localization are toted . ^^^ The singularities of synthesis of PAI 1 and tPA , possible paths of regulation of their activity briefly surveyed . ^^^ Known polymorphisms of PAI 1 gene and tPA gene , their localization , frequencies in different ethnic groups are circumscribed . ^^^ In operation the genetic influence of polymorphisms of PAI 1 gene and tPA gene on synthesis and plasma concentration of PAI 1 and tPA , including under an operation of risk factors of atherosclerosis , and as possible clinical consequences of sectional violations is featured . ^^^ In the review the possible role of polymorphisms of PAI 1 gene and tPA gene in a pathogenesis of atherosclerosis , association with hazard thrombotic complications for different diseases is considered . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Preliminary results show that , among patients with short aPTTs , homozygosity for the hyperfibrinolytic PAI 1 5G or tPA 1 alleles are at increased risk of in hospital mortality compared with 4G / 4G PAI 1 and D / D tPA homozygotes ( OR=2 . 6 , 95 % CI : 1 . 3 5 . 5 and OR=5 . 5 , 95 % CI : 1 . 3 24 . 5 , respectively ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The degradation of basement membranes by tumor cells involves secretion and activation of proteinases , such as matrix metalloproteinases ( MMPs ) and the plasminogen activation system ( uPA , tPA , PAI 1 ) , and results from an imbalance between their inhibitors and activators , controlled by various growth factors or cytokines . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : To observe the effects of exercise training on plasminogen activator inhibitor , type 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) , and associated metabolic variables in sedentary men and women . ^^^ Blood samples were collected on days 1 , 2 , 11 , and 12 for measurement of plasma PAI 1 , tPA , insulin , glucose , and triglyceride . ^^^ RESULTS : There were no significant changes in PAI 1 , tPA , or associated metabolic variables between EX and CON during the intervention period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of tissue plasminogen activator ( tPA ) and is elevated in diseases of vascular remodeling . ^^^ In a biochemical assay determining direct tPA activity , human recombinant PAI 1 completely inhibited tPA , but this inhibition was blocked by WAY 140312 at an IC ( 50 ) of 15 . 6 microM . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of tissue plasminogen activator ( tPA ) and is elevated in diseases of vascular remodeling . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We compared plasma levels of interleukin ( IL ) 6 , C reactive protein ( CRP ) , IL 1beta , IL 11 , tissue plasminogen activator ( tPA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) activity , and global fibrinolytic capacity ( GFC ) in patients with MM ( n = 66 ) and in control subjects ( n = 18 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Supernatant was removed for analysis at 0 , 24 , 48 , and 72 hours after gas incubation and analyzed for plasminogen activator activity , total tissue plasminogen activator ( tPA ) , and total plasminogen activator inhibitor 1 ( PAI 1 ) concentrations by use of an enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We studied the activities of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) and the levels of tPA antigen ( a marker of endothelial dysfunction ) in 551 subjects with normal glucose tolerance in 1990 in relation to incident diabetes during nine years of follow up . ^^^ RESULTS : Subjects with diabetes at follow up ( n = 15 ) had significantly lower baseline tPA activity and higher PAI 1 activity and tPA antigen than non converters . ^^^ The risk of diabetes with low tPA activity or high PAI 1 activity persisted after adjustment for age and sex but diminished to a non significant level after further adjustments . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Renal gene expressions of type 1 plasminogen activator inhibitor ( PAI 1 ) , tissue type PA ( tPA ) , and urokinase type PA ( uPA ) were examined by real time PCR . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis was determined by tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) and fibrin degradation product ( D dimer ) . ^^^ A significant as well as defective fibrinolysis was also seen with increased levels of tPA and D dimer unopposed by PAI 1 after one week and also after 30 days . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To assess the relationship of these AGEs to endothelial function and inflammation , markers of endothelial function von Willebrand factor ( vWf ) , soluble vascular cellular adhesion molecule 1 ( sVCAM 1 ) , soluble E selectin ( sE selectin ) , soluble thrombomodulin ( sTM ) , tissue type specific plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , and C reactive protein ( CRP ) , a marker of inflammatory activity , were determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TPA and PAI 1 were found to be significantly raised in the course of pre eclampsia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prior work , using a mixed species co culture system , has shown astrocyte regulation of brain capillary endothelial expression of key hemostasis factors tissue plasminogen activator ( tPA ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Following 48 h co culture , we analyzed both endothelial mono cultures and astrocyte endothelial co cultures for expression of tPA and PAI 1 mRNA , protein , and activity . ^^^ RESULTS AND CONCLUSIONS : There were significant changes for both tPA and PAI 1 mRNA : tPA mRNA levels were decreased in co cultures ( 55+ / 16 % of mono cultures , p < 0 . 0005 ) and PAI 1 mRNA levels were increased 144+ / 38 % , compared to mono cultures ( p < 0 . 005 ) . ^^^ Co cultures produced a 54 % reduction in tPA protein ( 12 . 7+ / 3 . 8 vs . 27 . 5+ / 7 . 1 ng / ml , p < 0 . 005 ) and a 24 % increase in PAI 1 protein ( 117 . 5+ / 3 . 2 vs . 94 . 9+ / 5 . 9 ng / ml , p < 0 . 0005 ) . ^^^ TGF beta neutralizing antibody attenuated the observed changes in both tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and such plasminogen activation system components as uPA , PAI 1 and tPA were determined by enzyme immunoassay methods in endometrial tumors from 121 patients and 18 samples of endometrial hyperplasia of varying degree . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The BBB endothelium also expresses tissue plasminogen activator ( tPA ) , a key protein in fibrinolysis , and its rapid inhibitor , plasminogen activator inhibitor ( PAI 1 ) . ^^^ In particular , functional changes caused by major stroke risk factors in the TM protein C , tPA / PAI 1 , and tissue factor systems at the BBB may result in large and debilitating infarctions following an ischemic insult . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Average tissue plasminogen activator ( tPA ) levels were significantly lower in the HAC group by 30 min on CPB ( P < 0 . 05 ) , resulting in preservation of plasminogen activator inhibitor ( PAI ) 1 during CPB ( P < 0 . 05 ) . ^^^ Overall , the combined strategy was associated with a reduction in CPB induced increases in markers of thrombin generation , fibrin formation , tPA release , and fibrin degradation and better preservation of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Laboratory tests included plasma assays of fibrinogen , tissue plasminogen activator ( TPA ) , D dimer , plasminogen activator inhibitor 1 ( PAI 1 ) , plasminogen and factor 7 : c . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the association of FV G1691A mutation , F 2 G20210A gene polymorphism ( PM ) , 4G / 5G PAI 1 and Alu I / D tPA PM in 32 women with infertility and 49 women with at least 2 unexplained early abortions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity in peritoneal fluid was assayed by zymography , and expression of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 , both regulators of fibrinolytic activity , was assessed in peritoneal tissue and fluid by RT PCR and bioassay , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CONCLUSIONS : Baseline PAI 1 and TAFI levels predict SICH after stroke tPA therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , enzyme linked immunosorbent assays ( ELISA ) of uPA antigen , and the activities of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were not statistically different from those in control experiments . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hormone induced coordinated expression of tissue type PA ( tPA ) produced mainly by granulosa cells and its inhibitor PAI 1 secreted by theca cells in the preovulatory follicles is responsible for a controlled and directed proteolysis leading to the rupture of selected follicles in the rat , monkey and other mammals . ^^^ Increase in tPA and PAI 1 expression in corpus luteum ( CL ) of rat and monkey at a later stage is well correlated with a sharp decrease in CL progesterone production , indicating its important role in the initiation of luteal regression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin fragment 1 + 2 , fibrinogen , factor 8 , antithrombin , tissue plasminogen activator antigen ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , PAI 1 / tPA , and C reactive protein were significantly ( P < 0 . 05 ) higher in the hyper than in the hypothyroid state , whereas protein C and plasmin antiplasmin complexes were significantly lower during the hyperthyroid period . ^^^ When the 10 female patients were hyperthyroid , their levels of prothrombin fragment 1 + 2 , fibrinogen , protein S , antithrombin , tPA , PAI 1 , and PAI 1 / tPA were significantly higher ( P < / = 0 . 05 ) than in healthy female controls , whereas when the female patients were hypothyroid , their antithrombin and plasmin antiplasmin were lower and their protein S was higher than in controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma levels of plasminogen activator inhibitor 1 ( PAI 1 ) antigen , tissue plasminogen activator inhibitor ( tPA ) antigen , thrombomodulin ( TM ) , tissue factor pathway inhibitor ( TFPI ) antigen , and fibrinogen were determined before and after 6 months of therapy . ^^^ Treatment with both drugs was associated with a significant decrease in PAI 1 antigen , TM , fibrinogen plasma levels and an increase in tPA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HIV infected subjects who have lipodystrophy and insulin resistance on prolonged antiretroviral therapy have elevated levels of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigens , markers of impaired thrombolysis that are associated with hyperinsulinemia and increased cardiovascular risk . ^^^ Levels of tPA antigen declined by approx 25 % with both treatments ( p < 0 . 05 for each ) ; levels of PAI 1 antigen did not change . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Bleeding time , platelet count , prothrombin time , activated partial thromboplastin time , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI 1 ) , euglobulin clot lysis time , protein C , protein S , fibrinogen , D dimer , factor 5 , 7 , 8 , 9 , 10 and von Willebrand factor ( VWF ) values were studied at the beginning , at 2 and 4 h of dialysis with and without administration of DDAVP at a dose level of 2 microg / kg intranasally . ^^^ After DDAVP administration , bleeding time , PAI 1 levels were significantly lower ( p < 0 . 01 ) , while tPA , factor 8 and VWF levels increased significantly ( p < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis was quantified by measurement of the activity levels of tissue plasminogen activator ( tPA , the activator of fibrinolysis ) and its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) , using an amidolytic method . ^^^ After 6 months of exercise , subjects in the IC EX group experienced significant improvements in fibrinolytic activity , manifested as a 23 % decrease in PAI 1 activity and a 28 % increase in tPA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated the effects of ARBs on TF activity , tissue plasminogen activator ( tPA ) , PAI 1 antigen levels , plasma renin activity ( PRA ) and aldosterone levels in hypertensive patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to inhibiting serine proteases ( mainly tPA and uPA ) , PAI 1 interacts with vitronectin ( Vn ) , fibrin or alpha ( 1 ) acid glycoprotein , interactions which are important for PAI 1 mediated effects in inflammation , tumor invasion and metastasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Laser capture microdissection ( LCM ) was combined with plasminogen casein zymography to analyze uPA , tissue PA ( tPA ) , uPA PAI 1 complexes , and tPA PAI 1 complexes in 11 tumors and adjacent non malignant epithelium from squamous cell carcinomas of the tongue , floor of mouth , larynx , and vocal cord . uPA was detectable in all tumor samples analyzed , uPA PAI 1 complexes in three samples , and tPA in nine . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Temporal changes in tPA and PAI 1 after maximal exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined the effect of homocysteine lowering by B vitamin supplementation on tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI ) and von Willebrand factor ( vWf ) markers of endothelial dysfunction in hyperhomocysteinemic and normohomocysteinemic volunteers . ^^^ Before and after the intervention period , blood samples were taken for measurements of homocysteine , tPA , PAI and vWf . ^^^ Concentrations of tPA , PAI and vWf did not change after supplementation of B vitamins . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As a result of major bleeding , the fibrinolytic system was also activated : a biphasic elevation of tPA was seen , first immediately after the bleeding event at 2 h , and later on the first postoperative day , followed by fibrinolytic shutdown associated with increased PAI 1 values . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim was to investigate if the local availability of active tPA on the organ level depends on the local release rate of tPA or the arterial input of tPA and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ Forearm availability of active tPA was not related to arterial input of either tPA or PAI 1 . ^^^ The major determinant for the local availability of active tPA is the capacity of the endothelium to release tPA rather than the arterial input of PAI 1 or tPA . ^^^ Despite a molar excess of PAI 1 , the majority of tPA released during stimulation does not undergo local inactivation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Left atrial and peripheral venous levels of fibrinogen , antithrombin 3 , factor 7 and factor 8 for coagulation , D dimer , tPA and PAI 1 , plasmin and antiplasmin for fibrinolysis , and platelet factor 4 and vWF for platelet activation , and endothelial dysfunction were measured in 46 patients with MS and normal clotting times who were undergoing percutaneous mitral valvuloplasty . ^^^ Left atrial tPA , plasmin , PAI 1 , antiplasmin , PF 4 , and vWF levels exceeded the corresponding peripheral venous levels ( P < 0 . 05 ) in patients with MS , being more significant in the AF subgroup . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The main components in plasminogen activation include plasminogen , tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , urokinase plasminogen activator receptor ( uPAR ) , and plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The binding of tPA and plasmin to S100A10 also protects against inhibition by physiological inhibitors , PAI 1 and alpha 2 antiplasmin , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A repeated measures analysis of variance ( ANOVA ) with a between subjects factor revealed significant differences in post transplant levels of antithrombin 3 , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and D dimer according to the occurrence of VOD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured fibrinogen , D dimer , antithrombin 3 ( ATIII ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) activity , and factor 7 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic variables tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) activity , and antigen and prothrombin fragment 1 + 2 , a molecular marker of in vivo thrombin generation , were measured before and after maximal exercise . ^^^ Higher tPA and lower PAI 1 activities ( P < 0 . 05 ) were seen in HRT users and fit groups . tPA and PAI 1 antigens were lower in HRT and fit groups ( P < 0 . 05 ) but not after correction for body mass index . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effects of short term atorvastatin treatment on forearm vasodilatory response to reactive hyperaemia ( RH % ) and on components of the thrombosis fibrinolysis system ( antithrombin 3 , proteins and S , factors 5 and 7 , von Willebrand factor , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI 1 ) ) in patients with heart failure . ^^^ Plasma concentrations of antithrombin 3 , protein C , protein S , factor 5 , factor 7 , von Willebrand factor , tPA , and PAI 1 were determined before and after treatment . ^^^ CONCLUSION : Atorvastatin did not change the maximum hyperaemic flow , although it decreased plasma concentrations of antithrombin 3 , protein C , factor 5 , tPA , and PAI 1 in patients with heart failure . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of tissue PA ( tPA ) , urokinase PA ( uPA ) and PAI 1 were measured by an enzymatic immunity method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULT ( S ) : In patients with endometriosis and adhesions , significantly higher peritoneal fluid concentrations were found for plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) , and plasminogen , compared with patients with endometriosis but without adhesions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
INTRODUCTION : The purpose of this study was to estimate , in patients undergoing cardiopulmonary bypass ( CPB ) , the in vivo rates of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) secretion , plasmin generation , fibrin degradation , and plasmin inhibition by aprotinin versus antiplasmin . ^^^ MATERIALS AND METHODS : Estimates of in vivo rates were based on measured levels of tPA , PAI 1 , antiplasmin , plasmin antiplasmin complex ( PAP ) , total aprotinin , plasmin aprotinin complex and D dimer , combined with a computer model of each patient ' s vascular system that continuously accounted for secretion , clearance , hemodilution , blood loss and transfusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In them , and in an equal number of controls , plasma levels of immunoreactive tissue plasminogen activator ( tPA ) , plasminogen activation inhibitor 1 ( PAI 1 ) , von Willebrand factor ( VWF ) , fibrinogen , D dimer and CRP were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The remainder of the patients had a thrombophilia as follows : 195 ( 60 % ) had antiphospholipid syndrome , 64 ( 20 % ) had Sticky Platelet Syndrome , 38 ( 12 % ) had MTHFR mutation , 23 ( 7 . 1 % ) had PAI 1 polymorphism , 12 ( 3 . 7 % ) had Protein S deficiency , 12 ( 3 . 7 % ) had Factor 5 Leiden , 3 ( 1 % ) , had AT deficiency , 3 ( 1 % ) had Heparin Cofactor 2 deficiency , 3 ( 1 % ) had TPA deficiency , and 6 ( 2 % ) had Protein C deficiency . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) are two important mediators of fibrinolysis . ^^^ Plasma levels of PF 4 , BTG , PAI 1 , and tPA may show changes in chronic inflammatory diseases such as asthma . ^^^ Plasma levels of PF 4 , BTG , PAI 1 , tPA were determined before starting steroid therapy and after receiving 1 week of steroid therapy . ^^^ Plasma levels of tPA and PAI were statistically higher than those in controls in asthmatic patients on admission ( 6 . 01+ / 2 . 72 vs . 5 . 4+ / 2 . 3 ng / mL for tPA and 75 . 2+ / 27 . 2 ng / mL vs . 32 . 7+ / 14 . 3 ng / mL for PAI 1 ) . ^^^ Further increases were detected in two parameters after 1 week of therapy with steroids ( tPA levels were 6 . 85+ / 2 . 96 ng / mL and PAI 1 levels were 83 . 5+ / 29 . 6 ng / mL ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased levels of tPA , uPA , uPA receptor ( uPAR ) , and their inhibitor , plasminogen activator inhibitor ( PAI ) 1 , have been found in the cerebrospinal fluid ( CSF ) of patients with bacterial meningitis . ^^^ Here , we show that expression of tPA , uPA , uPAR , PAI 1 , and PAI 2 is up regulated during experimental pneumococcal meningitis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction assessed mRNA levels of eNOS , tPA , and the endogenous plasminogen activator inhibitor PAI 1 . ^^^ Statins increased eNOS and tPA mRNA levels but did not change mRNA levels of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
LysoPC also induced the mRNA expression of urokinase type plasminogen activator ( uPA ) , uPA receptor , and plasminogen activator inhibitor 1 , but the kinetics were different from that of tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Testosterone does not adversely affect fibrinogen or tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) levels in 46 men with chronic stable angina . ^^^ Measurements of free , total and bioavailable testosterone , luteinising hormone ( LH ) and follicle stimulating hormone ( FSH ) , estradiol , plasminogen activator inhibitor 1 ( PAI 1 ) , fibrinogen , tissue plasminogen activator ( tPA ) and full blood count were made at 0 , 6 and 14 weeks . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here we examined whether the plasminogen activator inhibitor 1 and 2 ( PAI 1 and PAI 2 ) mRNA , endogenous inhibitors of tPA and uPA , are induced in the DRG following sciatic nerve transection . ^^^ Co expression of PAI 1 , 2 with tPA and uPA in DRG neurons suggests that these inhibitors may act in an autocrine manner to modulate extracellular proteolytic activity after nerve injury . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of plasminogen activity , uPA , tissue plasminogen activator ( tPA ) , and their inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) were measured in tears and plasma of patients with VKC . ^^^ The presence of tPA , uPA , and urokinase receptor ( uPAR ) in conjunctival tissues were evaluated by immunohistochemistry . uPA , uPAR , and PAI 1 expression and production were measured in conjunctival epithelial cell and fibroblast cultures treated with cytokines . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A coordinated increase in tPA and its inhibitor PAI 1 expression in the monkey and rat CL may be instrumental in initiating luteal regression in both species , and correlated well with the timing of the closure of the implantation window , whereas high uPA activity in the CL is important for the early formation of the CL and for maintaining its function which is closely correlated to the period of establishment of the implantation window . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our results suggest that limited availability of tPA in MS lesions because of formation of tPA plasminogen activator inhibitor 1 complexes reduces capability of tPA receptors to generate plasmin , which further diminishes fibrinolytic capacity in active MS lesions and possibly leads to axonal damage . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma P selectin antigen , tissue plasminogen activator ( tPA ) antigen and activity , and plasminogen activator inhibitor 1 ( PAI 1 ) activity were determined in 60 acute atherosclerotic stroke patients and matched control subjects . ^^^ The levels of P selectin , tPA antigen , and PAI 1 activity were all significantly higher in stroke patients compared with controls ( all p < 0 . 0001 ) ; the level of tPA activity was significantly lower in patients than that in controls ( p < 0 . 0001 ) . ^^^ In stroke group , P selectin concentration was highly correlated to PAI 1 activity ( r = 0 . 8433 , p < 0 . 001 ) , but not to tPA antigen ( r = 0 . 1752 , p > 0 . 05 ) , and tPA activity ( r = 0 . 2465 , p > 0 . 05 ) , which was further confirmed in the multiple linear regression analysis ( F = 47 . 052 , p < 0 . 0001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The complex between tPA and PAI 1 : risk factor for myocardial infarction as studied in the SHEEP project . ^^^ INTRODUCTION : The tPA / PAI 1 complex seems to be an important biochemical marker for myocardial reinfarction . ^^^ Therefore we explored the distribution , correlation and interaction of plasma concentrations of tPA / PAI 1 complex in all available patients and matched controls in the Stockholm Heart Epidemiology Program ( SHEEP ) . ^^^ The study consists of 886 ( 591 men and 295 women ) patients and 1198 ( 753 men and 445 women ) matched controls , who were all analysed for plasma tPA / PAI 1 complex . ^^^ RESULTS : The plasma concentration of tPA / PAI 1 complex was significantly associated with the risk for MI , for both genders . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen activator ( PA ) system comprises the 2 serine proteases , urokinase PA ( uPA ) and tissue PA ( tPA ) , the 2 serpin inhibitors , PAI 1 and PAI 2 and the uPA receptor ( uPAR ; CD 87 ) . ^^^ High levels of uPA , PAI 1 , uPA PAI 1 complex and uPAR in breast cancer tissue are associated with poor prognosis , while high levels of tPA or PAI 2 correlate with good prognosis . ^^^ In this study , pre operative plasma levels of uPA , PAI 1 , uPAR , tPA , uPA PAI 1 complex , and tPA PAI 1 complex were measured in patients with benign ( n=103 ) and malignant breast disease ( n=113 ) by immunoenzymatic assays ( ELISA ) . ^^^ While plasma antigen levels of uPA , PAI 1 , uPA PAI 1 complex and uPAR were not significantly different in the 2 groups , antigen levels of tPA and tPA PAI 1 complex were significantly higher in patients with breast carcinoma compared to the control group . ^^^ In plasma from the breast cancer patients , uPA levels correlated weakly but significantly with those of tPA ( r=0 . 20 , p=0 . 035 ) and uPAR ( r=0 . 208 , p=0 . 028 ) . tPA levels correlated strongly with tPA PAI 1 complex ( r=0 . 972 , p=0 . 0001 ) while uPA PAI 1 levels were significantly associated with PAI 1 levels ( r=0 . 534 , p < 0 . 0001 ) , tPA levels ( r=0 . 348 , p=0 . 0003 ) and tPA PAI 1 levels ( r=0 . 356 , p=0 . 002 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we examined the effects of microinjections of plasminogen activator inhibitor 1 ( PAI 1 ) , tPA or plasmin into the NAc on morphine induced dopamine release , hyperlocomotion and anti nociceptive effects in ICR mice . ^^^ The intracerebroventricular injection of PAI 1 , tPA and plasmin at high doses had no effect on the anti nociceptive effects of morphine . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the role of tissue plasminogen activator ( tPA ) activity and plasminogen activator inhibitor type 1 ( PAI 1 ) in survivors of a first myocardial infarction ( MI ) . ^^^ RESULTS : PAI 1 activity , fibrinogen , postload insulin and proinsulin were significantly higher and tPA activity significantly lower in MI patients in the univariate analysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
VEC null cells also present an altered fibrinolytic activity with increases in tPA , uPA , uPAR and a strong reduction in PAI 1 , which may be correlated to the high incidence of abrupt hemorrhages in VEC null tumors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As a marker of hemostatic system fibrinogen , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) levels were measured initially , and after 1 and 6 months of therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Scarce and conflicting data exist about the effects of diuretics and beta adrenoceptor antagonists on the fibrinolytic system , whereas ACE inhibitors have generally been reported to improve the fibrinolytic balance by decreasing plasma PAI 1 levels , calcium channel antagonists have been shown to increase tissue plasminogen activator ( tPA ) activity , and angiotensin 2 type 1 ( AT ( 1 ) ) receptor antagonists seem to exert neutral effects . beta Adrenoceptor antagonists , calcium channel antagonists , and AT ( 1 ) receptor antagonists have been reported to exert anti aggregatory effects on platelets , while contrasting data exist about the influence of ACE inhibitors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here , we report that prostate carcinoma cells LNCaP and PC 3 autoactivate latent full length PDGF D into its active form under serum independent conditions and that this autoactivation is inhibited by PAI 1 , a urokinase plasminogen activator ( uPA ) / tissue plasminogen activator ( tPA ) inhibitor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity was determined by assaying tissue plasminogen activator ( TPA ) and plasminogen activation inhibitor type 1 ( PAI 1 ) . ^^^ Levels of the inhibitory and fibrinolytic factors including protein C , protein S , APC R , TPA and PAI 1 remained stable . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endotoxin ( lipopolysaccharide ( LPS ) ) , used as a standardized stimulus to define brain specific inflammation induced change , amplified pericyte induced enhanced release of the tPA inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) ; the latter was released by endothelial cells first cocultured with pericytes and then incubated with LPS in the absence of pericytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effects of LDL glycation on endothelial cell secretion of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) have not been fully elucidated . ^^^ Human aortic endothelial cell ( HAEC ) tPA and PAI 1 production were determined after incubation with LDL ( 50 to 500 microg / mL protein , 24 h ) from three sources : ( 1 ) nondiabetic LDL ( N LDL ) modified in vitro to form six preparations : native , nonmodified ( N ) ; glycated ( G ) ; minimally oxidized ( MO ) ; minimally oxidized and glycated ( MOG ) ; heavily oxidized ( HO ) ; and heavily oxidized and glycated ( HOG ) ; ( 2 ) in vivo glycated and relatively nonglycated LDL subfractions from type 1 diabetic patients ; ( 3 ) LDL from type 1 diabetic patients and matched controls , which was subfractionated using density gradient ultracentrifugation . ^^^ Results using in vivo glycated versus nonglycated LDL showed that tPA and PAI 1 release did not differ between subfractions . ^^^ Release of tPA averaged 5 . 11 + / 0 . 6 and 5 . 12 + / 0 . 7 ng / mg / 24 h , whereas release of PAI 1 averaged 666 + / 27 ng / mg / 24 h and 705 + / 30 ng / mg / 24 h for nonglycated and glycated LDL subfractions , respectively . ^^^ Using LDL of different density subclasses , tPA and PAI 1 release in response to LDL from diabetic patients compared with control subjects did not differ when HAECs were incubated with LDLs of increasing density isolated from each subject pair . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using enzyme linked immunosorbent assays , we determined vascular cell adhesion molecule 1 ( sVCAM 1 ) , sE selectin , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type specific plasminogen activator ( tPA ) , von Willebrand factor ( vWF ) , and soluble thrombomodulin ( sTM ) to be markers of endothelial function and determined C reactive protein ( CRP ) to be a marker of inflammation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To study the role of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( tPA ) in the accumulation of extracellular matrix ( ECM ) in the kidney of KKAy mice with type 2 diabetes . ^^^ CONCLUSION : In type 2 diabetes KKAy mice , the accumulation of ECM may be associated with the abnormal expression of PAI 1 / tPA mRNA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the 14th day adhesions were evaluated and tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) type 1 and 2 were measured in peritoneal biopsy specimens . ^^^ Nevertheless , there were no difference between the two groups in levels of tPA , PAI 1 and PAI 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Fibrinolysis in blood is mainly reflected by the activities of tissue plasminogen activator ( tPA ) and of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ METHODS : In six anesthetized pigs , we occluded the distal left anterior descending coronary artery ( LAD ) four times for 10 min with 40 min intervals and determined the activities of tPA and PAI 1 in arterial and coronary venous blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of prothrombin fragment 1+2 ( F1+2 ) , factor 7 , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue factor pathway inhibitor ( TFPI ) antigens , but not tissue plasminogen activator ( tPA ) antigen , in the pre simvastatin plasmas of the diabetic patients were significantly higher than the levels found in plasmas of healthy subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : After 24 hours ' incubation , tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) levels were determined in medium and cell lysates by using ELISA techniques . ^^^ RESULT ( S ) : In medium of co cultures , tPA and PAI 1 concentrations were statistically significantly increased compared with the case of monocultures , whereas uPA concentration was statistically significantly decreased . ^^^ In cell lysates of co cultures , PAI 1 concentration was statistically significantly increased compared with the case of monocultures , whereas tPA and uPA were unaffected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of high density lipoprotein and oxidized high density lipoprotein on the synthesis of NO , the activity of NOS , and the secretion of tPA and PAI 1 by cultured human umbilical vein endothelial cells ( HUVEC ) . ^^^ The levels of NO , the activity of NOS , and the levels of tPA and PAI 1 in the medium were detected after the incubation . ^^^ RESULTS : Compared with the 0 . 01 mol / L PBS control , the synthesis of NO ( P < 0 . 05 ) and the activity of NOS ( P < 0 . 01 ) were significantly increased in the N HDL group , but the change of tPA and PAI 1 displayed no statistical significance ( both P > 0 . 05 ) . ^^^ In the OX HDL group , the synthesis of NO and the activity of NOS were significantly decreased in a dose dependent way ; the PAI 1 activity ( P < 0 . 05 ) was increased , but the secretion of tPA ( P > 0 . 05 ) was not affected . ^^^ CONCLUSION : N HDL significantly increased the synthesis of NO and the activity of NOS secreted by HUVEC , but it had no influence on the generation of tPA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On univariable analysis , fibrinogen , low activated protein C ratio , D dimer , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were associated significantly with risk of CVD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant associations were found for SNP 7 T to C and factor 7 activity , tPa concentrations , fibrinogen concentrations or factor 13 concentrations and SNP 20203 C to A and factor 7 concentrations , PAI 1 concentrations , tPa concentrations or fibrinogen concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A study of the serum levels of the plasminogen activator inhibitor 1 ( PAI 1 ) , the major inhibitor of tPA , found that women with MDD had a higher PAI 1 concentration than normal controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In vitro platelet aggregation induced by collagen and ADP , and variables of coagulation ( factor 7 amidolytic activity and concentrations of fibrinogen and prothrombin fragment 1 and 2 ) and fibrinolysis [ plasminogen activator inhibitor ( PAI ) activity and concentrations of tissue plasminogen activator ( tPA ) / PAI 1 complexes ] did not differ among the 3 diets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Impaired release of tPA and enhanced release of PAI 1 after endothelial activation is reported in patients with APS . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We analyzed cDNA expression by using the CNIO OncoChipTM , a cDNA microarray containing a total of 6386 genes represented by 7237 clones . uPA , uPAr , tPA , PAI 1 and PAI 2 were also studied at RNA and protein levels . ^^^ Microarrays of cDNA expression , RT PCR and Western blot performed in IMR 90 E1A expressing cells showed downregulation of uPA , uPAr , tPA , PAI 1 and upregulation of PAI 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Arsenic / TPA treatment resulted in increased expression of alpha fetoprotein , k ras , c myc , estrogen receptor alpha , cyclin D 1 , cdk2na , plasminogen activator inhibitor 1 , cytokeratin 8 , cytokeratin 18 , glutathione S transferases and insulin like growth factor binding proteins in liver and liver tumors from both male and female mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We summarize the data on the role of leptin , adiponectin , the growth hormone axis , glucocorticoids , sterol response element binding protein 1c ( SREBP 1c ) , the tumor necrosis factor alpha axis ( TNF alpha ) , interleukin 6 ( IL 6 ) , interleukin 18 ( IL 18 ) , interferon alpha ( IFN alpha ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI 1 ) in the pathophysiology of LD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The abundance of mRNA encoding PN 1 , tissue type PA ( tPA ) , urokinase ( uPA ) and PA inhibitor 1 ( PAI 1 ) were initially upregulated by human chorionic gonadotropin ( hCG ) in bovine preovulatory follicular wall homogenates . ^^^ PN 1 , PAI 1 and tPA mRNA expression then decreased near the expected time of ovulation , whereas uPA mRNA levels remained high . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We wanted to study plasminogen activators , urokinase ( uPA ) and tissue type ( tPA ) and their inhibitor PAI 1 , which have not earlier been studied comprehensively in cutaneous NF 1 related tumors . ^^^ We analyzed the distribution of uPA , tPA and PAI 1 antigen level by immunohistochemistry and mRNA level by in situ hybridization , to identify which cells are primarily involved in proteolytic activity and plasminogen activation . ^^^ Large extent of tumor cells of Schwann cell origin and prominent expression levels of uPA , tPA and PAI 1 indicated that these cells are responsible for the main source of PA components in cutaneous NF 1 related neurofibromas . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activities of tissue and urokinase plasminogen activators ( tPA and uPA , respectively ) , the relative inhibition of tPA , and the amounts of plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 , respectively ) in cell free saliva were studied . ^^^ The activities of tPA and uPA , and tPA inhibition , were measured using in house microtiter plate assays , and PAI 1 and PAI 2 levels were measured using commercial enzyme linked immunosorbent assay ( ELISA ) kits . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hemostatic factors associated with the development of cardiovascular disease ( CVD ) include fibrinogen , von Willebrand factor , tissue plasminogen activator ( tPA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) , and factor 7 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Primary outcome measures were plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) and D dimer levels . ^^^ RESULTS : First degree relatives had significantly higher mean PAI 1 ( 10 . 23 versus 7 . 85 ng / ml ; P = 0 . 024 ) , tPA ( 9 . 98 versus 8 . 29 ng / ml ; P < 0 . 001 ) and D dimer levels ( 56 . 6 versus 46 . 1 ng / ml ; P = 0 . 004 ) . ^^^ CONCLUSION : Raised levels of PAI 1 , tPA , D dimer and estimated insulin resistance were present in the healthy male first degree relatives of men with intermittent claudication . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In a cell free system , using tissue plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) to activate plasminogen , PAI 1 inhibited plasmin generation induced by both activators , whereas IGFBP 5 prevented the effects of PAI 1 on tPA but not uPA . ^^^ Furthermore , we noted that IGFBP 5 activated plasminogen to a greater extent than could be explained solely by inhibition of PAI 1 , suggesting that IGFBP 5 could directly activate tPA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured insulin sensitivity ( euglycemic clamp ) , forearm blood flow responses to graded local acetylcholine ( Ach ) and sodium nitroprusside ( SNP ) infusions , plasma concentrations of tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 6 , von Willebrand factor ( vWF ) , plasminogen activator inhibitor ( PAI ) 1 , tissue plasminogen activator ( tPA ) , and high sensitivity C reactive protein ( hs CRP ) in 81 diabetic patients . ^^^ In multivariate analysis , inflammatory markers ( IL 6 , hs CRP , and TNF alpha ) were independently associated with insulin resistance and fasting glycemia , fibrinolytic markers PAI 1 and tPA with insulin resistance and central fat distribution , and vascular indexes ( vWF , Ach , and SNP vasodilation ) with insulin resistance and obesity or cytokines ( TNF alpha or IL 6 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endocytic receptor LRP together with tPA and PAI 1 coordinates Mac 1 dependent macrophage migration . ^^^ Subsequent neutralization of tPA by its inhibitor PAI 1 enhances binding of the integrin protease inhibitor complex to the endocytic receptor LRP ( lipoprotein receptor related protein ) , triggering a switch from cell adhesion to cell detachment . ^^^ Genetic inactivation of Mac 1 , tPA , PAI 1 or LRP but not the protease uPA abrogates macrophage migration . ^^^ In vitro analysis shows that tPA promotes Mac 1 mediated adhesion , whereas PAI 1 and LRP facilitate its transition to cell retraction . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As a consequence of thrombin dependent inactivation of PAI 1 , tPA induced fibrin clot lysis time in the presence of PAI 1 was shortened by unfractionated heparin as well as by LMW heparin with lesser extent , which was further enhanced by Vn . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activities and concentrations of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tPA / PAI 1 complex and hydroxyproline contents were determined . ^^^ The tPA / PAI 1 complex levels were similar among the three groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of our study was to assess the impact of WCH on fibrinolytic balance and endothelial function by measuring plasma levels of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator antigen ( tPA ) , fibrinogen , and thrombomodulin . ^^^ Subjects with WCH were found to have increased plasma levels of PAI 1 , tPA , fibrinogen and thrombomodulin compared to controls , but less compared to hypertensive ones . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasmin , tissue plasminogen activator ( tPA ) , and urokinase plasminogen activator ( uPA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) , and alpha 2 antiplasmin ( alpha2AP ) antigen were measured in the AAA wall and juxtamural and luminal aspects of intraluminal thrombus in 18 patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
UPA , UPAR , ECE 1 , PAFAH1B1 , PGT , INOS , ENOS , TPA , ICAM 1 , VCAM 1 , PAI 1 , PAI 2 , VWF , PTGDR , F 3 , THBD ) , which was most evident after 24 hours . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , LDLs have been implicated in the induction of changes in permeability , cell adhesion and secretion of vasoactive molecules ( nitric oxide [ NO ] ) , while VLDLs seem to modulate the fibrinolytic system [ tissue plasminogen activator ( TPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A hexapeptide ( EEIIMD ) corresponding to amino acids 350 355 of plasminogen activator inhibitor type 1 ( PAI 1 ) abolished the tPA induced increase in infarct size and intracranial bleeding in both mechanical and embolic models of stroke in rats , and reduced brain edema and neuronal loss after traumatic brain injury in pigs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Group assignment was not found to be a determinant of waist circumference , high sensitive C reactive protein ( hs CRP ) , fibrinogen , von Willebrand factor ( vWF ) , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , tissue plasminogen activator ( tPA ) activity , tPA antigen , tPA PAI 1 complex , leptin , or HOMA 2 insulin resistance index ( HOMA 2 IR ) at follow up . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Real time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tPA PAI 1 ratio , an indicator of overall fibrinolytic activity . ^^^ RESULT ( S ) : Interceed treatment of normal peritoneal fibroblasts , adhesion fibroblasts , and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA PAI 1 ratio , signifying an overall increase in fibrinolytic activity . ^^^ CONCLUSION ( S ) : Interceed , which has been shown in multiple human in vivo studies to decrease postoperative adhesion development , increases the expression of tPA and the tPA PAI 1 ratio ( an indicator of overall fibrinolytic activity ) , thereby promoting dissolution of fibrin and healing without adhesion development . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelet monocyte binding ( a sensitive measure of platelet activation ) and plasma tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 , and von Willebrand factor concentrations were measured at intervals throughout the study . ^^^ In forearm resistance vessels , SFLLRN increased forearm blood flow ( P < 0 . 001 ) , tPA release ( P < 0 . 001 ) , and platelet monocyte binding ( P < 0 . 0001 ) without affecting plasma plasminogen activator inhibitor 1 or von Willebrand factor concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Neither the amount nor type of fat consumed influenced plasminogen activator inhibitor 1 or t plasminogen activator activities or D dimer concentration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIM OF THE STUDY : We studied the effects of fluvastatin and bezafibrate in monotherapy and in combination on plasma fibrinogen , t plasminogen activator inhibitor ( PAI 1 ) and C reactive protein ( CRP ) in patients with coronary artery disease ( CAD ) and mixed hyperlipidaemia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At rest , heparin and Org 10172 did not influence the plasma concentrations of endogenous t PA antigen and activity , urokinase type PA ( u PA ) antigen , plasmin activatable pro urokinase ( scu PA ) , active urokinase ( tcu PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An increased blood fibrinolytic activity manifested by increased tissue plasminogen activator ( t PA ) and decreased tissue plasminogen activator inhibitor ( PAI 1 ) and increased FDP levels are seen in 40 patients with mild hypertrophy of prostate . ^^^ Surgical treatment increased blood fibrinolytic activity manifested in the increase in t PA , decrease in PAI 1 , shortening of ELT , increase in FDP , and decrease in plasminogen and 2 AP activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The action of propionyl L carnitine on the vasal endothelium : increased t PA synthesis and a decrease in the activity of PAI 1 . ^^^ An important part is played by the reduction in t PA synthesis and the increase in PAI 1 activity , and the authors conclude that it is necessary to use pharmaceutical substances to restore proper equilibrium . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI complex was observed in most cases of APL , t PA did not seem to play an important role in the activation of fibrinolytic system in APL . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to evaluate the influence of dilution , pH and ionic strength on the precipitation of t PA and PAI 1 during euglobulin precipitation , we measured t PA Ag , PAI 1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients , under various conditions by changing pH , ionic strength and degree of dilution . ^^^ The precipitation of t PA Ag was not influenced by the dilution factor but the larger the dilution factor , the greater the PAI 1 and the smaller the fibrinolytic activity in the euglobulin fraction . ^^^ PAI Ag in euglobulin fraction showed consistent changes with t PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH . ^^^ The amount of precipitation of t PA and PAI 1 was increased by the presence of dextran sulfate , under varying pH , ionic strength and dilution conditions . ^^^ The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amount of t PA PAI 1 complex rather than minimized protease inhibitor in the euglobulin fraction . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The catabolism of t PA was not inhibited by an excess of urokinase type plasminogen activator ( u PA ) , and t PA bound to Novikoff membranes was not complexed to PAI 1 , suggesting a mechanism independent of PAI 1 . ^^^ Our results show that the clearance receptor for t PA on Novikoff cells is different from the mannose receptor and the PAI 1 dependent receptor described in other cells . ^^^ The rat hepatoma cells are thus a good model to study the PAI 1 independent hepatocyte specific clearance of t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , BM 06 . 022 and CHO t PA are inhibited by PAI 1 in a similar manner . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous studies with the human hepatoma cell line HepG 2 have identified a clearance system for t PA modulated by plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ In the present study , a rat hepatoma cell line MH1C1 is shown to contain a PAI 1 independent t PA clearance system . ^^^ When availability of matrix associated PAI 1 was blocked by preincubation with anti PAI 1 antibody or removed by elastase treatment , specific 125I t PA binding to MH1C1 cells was unaffected , whereas most of the specific 125I t PA binding to HepG 2 cells was abolished . ^^^ At 37 degrees C , PAI 1 independent t PA binding to MH1C1 cells was followed by ligand uptake and degradation with kinetics similar to that seen in HepG 2 cells . ^^^ Ligand receptor complexes generated by chemical cross linking were immunoprecipitable by anti t PA antibody but not by anti PAI 1 antibody , further supporting the finding that binding of t PA to MH1C1 cells is PAI 1 independent . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These properties have been attributed to its profibrinolytic activity [ increase of tissue plasminogen activator ( t PA ) activity , concomitant decrease of that of plasminogen activator inhibitor ( PAI ) ] , but there could conceivably be other factor ( s ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitor ( PAI 1 ) have been determined in endometrial curettings obtained from 46 subfertile women during proliferative , early or late secretory phases of the menstrual cycle . t PA activity and antigen concentrations was significantly higher ( P < 0 . 001 ) in late secretory endometrium than in proliferative or early secretory endometrium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitors ( PAI ) are elevated in late pregnancy with t PA and u PA remaining so at 6 weeks postnatal . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) levels in plasma and peritoneal effluent in patients on CAPD . ^^^ Antigenic PAI and t PA were determined . ^^^ Plasma t PA 6 . 64 + / 4 . 68 ( 2 . 4 20 ) ; Plasma PAI 1 24 . 8 + / 17 . 1 ( p < 0 . 001 in respect to controls ) ( 4 62 ) ; OPEt PA 1 . 46 + / 0 . 95 ( 0 . 4 4 . 6 ) ; OPE PAI 1 7 . 3 + / 5 . 6 ( 0 20 . 4 ) . ^^^ Peritoneal saturation ratios were for t PA 29 . 6 + / 21 % ( 6 65 ) and for PAI 1 34 + / 32 % ( 7 132 ) . ^^^ In conclusion our data do not support strong relationship between peritoneal t PA / PAI system and the functional characteristics of the peritoneal membrane although plasma PAI 1 , after an increase in patients at early stages on CAPD , shows a tendency to decrease over time and frequent peritonitis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Zymography of serial plasma samples from the bat PA treated dogs failed to demonstrate the apparent generation of a complex between bat PA and plasminogen activator inhibitor 1 ; the corresponding complex with t PA was observed in plasma samples from the t PA treated dogs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
An important role is represented by the t PA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to clarify the effect of hemodialysis ( HD ) on the fibrinolytic system , fibrinolytic activity was evaluated in 27 patients undergoing regular hemodialysis treatment ( RDT ) using new parameters including plasma alpha 2 plasmin inhibitor ( alpha 2 PI ) , alpha 2 plasmin inhibitor plasmin complex ( alpha 2 PIC ) , cross linked fibrin degradation products ( XL FDP ) , tissue plasminogen activator ( t PA ) activity , t PA antigen and plasminogen activator inhibitor 1 ( PAI 1 ) antigen . ^^^ Both t PA activity and t PA antigen significantly increased during HD , and PAI 1 antigen significantly decreased during HD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the peritoneal fluid of women with PID , PAI Ag , t PA Ag and u PA Ag were many times higher than in the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activators ( t PA , u PA ) and their inhibitor ( PAI 1 ) were studied in patients suffering from Buerger ' s disease and healthy volunteers before and after 15 minutes of venous occlusion test . ^^^ The fact that the reduced response of the endothelium to release t PA after venous stasis goes in parallel with marked decrease in PAI 1 antigen levels seems to suggest that patients suffering from Buerger ' s disease are not at high risk of intravascular fibrin deposition . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased levels of tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor ( PAI ) correlate with tumor necrosis factor alpha ( TNF alpha ) release in patients suffering from microangiopathy following allogeneic bone marrow transplantation ( BMT ) . ^^^ In order to analyze the pathophysiological events involved in microangiopathy , a prospective study comparing release of von Willebrand Factor ( vWF ) , t PA and PAI , as well as TNF alpha and further coagulation parameters was performed in 32 patients . ^^^ Endothelial damage as the central lesion was confirmed by the close association of vWF and t PA : Antigen with severity of microangiopathy . t PA activity , however , was neutralized by a simultaneous rise in PAI . ^^^ As clinical grades of microangiopathy , as well as the release of t PA : Ag and PAI were correlated with systemic release of TNF alpha our data further support our hypothesis of cytokine induced endothelial damage in clinical complications following allogeneic BMT . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and t PA / PAI 1 complex were measured in plasmas from 18 healthy subjects and 75 patients with various diseases ( 28 patients with haematological malignancies , 20 with thrombotic diseases , five with infectious diseases , four with liver diseases , ten with bleeding disorders and eight miscellaneous conditions ) . ^^^ Plasma levels of t PA antigen , PAI 1 antigen and t PA / PAI 1 complex were increased in the patients compared with the healthy subjects . t PA / PAI 1 complex levels correlated well with t PA antigen levels and molar concentrations of t PA antigen were similar to those of the t PA / PAI 1 complex . ^^^ Venous occlusion induced an increase in both t PA antigen and PAI 1 antigen and the molar concentration of the t PA / PAI 1 complex was equivalent to that of t PA antigen . ^^^ Following DDAVP infusion , the levels of t PA antigen and t PA / PAI 1 complex increased but PAI 1 antigen levels decreased , and the increase of t PA antigen was greater than that of t PA / PAI 1 complex . ^^^ These findings indicate that PAI 1 antigen exceeds t PA antigen in healthy subjects and in patients with various diseases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Immunologic analysis of the ability of PAI 1 to form complexes with immobilized t PA indicated that porcine and human platelets contained 3 . 7 + / 0 . 4 and 1 . 7 + / 0 . 3 U of PAI activity per 10 ( 8 ) platelets ( n = 6 ; + / SD ) , respectively ; sodium dodecyl sulfate ( SDS ) activation of the lysates increased PAI 1 activity to 10 . 8 + / 3 . 0 and 3 . 8 + / 0 . 5 U per 10 ( 8 ) platelets . ^^^ Platelet lysates were also treated with an excess of soluble t PA , which formed complexes with active PAI 1 , whereas the latent form was detected by SDS polyacrylamide gel electrophoresis and reverse fibrin autography . ^^^ Furthermore , immobilized t PA was able to deplete active PAI 1 from the platelet extracts , and the latent form remaining in the absorbed extract could be quantitated by activation with 4 mol / L guanidine . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine the relation of post percutaneous transluminal coronary angioplasty ( PTCA ) restenosis to serum lipid fractions and to circulating levels of endogenous tissue plasminogen activator ( t PA ) and its rapid inhibitor ( PAI 1 ) , 68 patients with coronary artery disease who underwent a successful PTCA were studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to study the effects of chronic venous hypertension due to heart failure on blood fibrinolytic activity , tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , t PA activity and PAI activity were measured before and after venous occlusion of the arm for 20 min in 15 patients with right sided heart failure , 15 patients with left sided heart failure , and 30 control healthy subjects . ^^^ There was no difference in the basal concentrations of t PA ( 11 . 0 , 10 . 2 and 10 . 8 ng / ml ; all values medians ) and PAI 1 antigens and their activities between right and left sided heart failure and the control subjects . ^^^ It was concluded that the poor increase in fibrinolytic activity that had already been reported in patients with heart failure , was due to low t PA release during occlusion and not to a high basal PAI level . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In animals with hepatic lesions induced by a 7 week inhalation of carbon tetrachloride there was a significant increase in blood t PA activity and PAI activity , with no significant change in portal pressure . ^^^ Following a 10 min portal vein occlusion there was a marked increase in portal pressure and t PA activity and a significant decrease in PAI activity . ^^^ Following ligation of both portal vein and hepatic artery , t PA activity increased to a higher extent and PAI activity was reduced to a lesser extent than changes found in portal stenosed rats . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HepG 2 membrane bound t PA was fully complexed to PA inhibitor 1 ( PAI 1 ) , whereas liver membrane bound t PA was not complexed . ^^^ Our results show that human liver membranes contain a specific 40 kDa binding protein for t PA that is different from the PAI 1 dependent receptor described on HepG 2 cells and the mannose receptor isolated from human liver . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were no differences in ECLT , PAI , PAI 1 : Ag or t PA : Ag throughout the arterial circulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 activity in plasma increased by a mean of 62 % in subjects randomized to receive n 3 PUFA despite that no changes could be demonstrated in t PA antigen levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This information suggests that a high concentration of t PA may be released into the narrow channels of the glands functioning to maintain duct patency under the regulation of an inhibitor such as plasminogen activator inhibitor ( PAI ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The latter was associated with elevated FXII and PKK , while C 1 INH was decreased , ATIII and alpha 2M were unchanged ; it could not be accounted for by changes in t PA , u PA , PAI , plasminogen , alpha 2 AP , proteins C or S . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The coronary patients had significantly higher plasminogen activator inhibitor ( PAI ) activity than the control subjects , while t PA antigen did not differ between the groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The mean levels of plasminogen , tissue plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) activity were significantly higher than in normal subjects , whereas the basal fibrinolytic activity was similar to that seen in the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the first and seventh administration days , the following parameters were evaluated : plasminogen activator inhibitor ( PAI 1 ) activity , PAI 1 antigen , tissue plasminogen activator ( t PA ) and whole blood , serum and plasma viscosity , before ( 0 time ) and 2 , 4 and 8 hours after ingestion of the drug . ^^^ Following the first administration ( 50 mg ) , a slight reduction of PAI was registered ; after 7 days ' administration , a clear cut lowering of functional and antigenic PAI was observed , together with an increase of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using chemical crosslinking , we have recently identified a plasminogen activator inhibitor type 1 ( PAI 1 ) independent t PA clearance receptor on rat hepatoma MH1C1 cells with a relative molecular mass of approximately 500 kDa . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Measurements included t PA , u PA , PAI 1 , PAP ( as a measure of in vivo activation of fibrinolysis ) , FbDPs , FGN , vWF , and 8 : C . ^^^ RESULTS In both IDDM and control subjects , levels of t PA , u PA , PAP , vWF , and 8 : C continued to rise throughout the exercise , whereas PAI 1 showed a similar decline in both groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The experiments were performed by adding 125I PAI 1 , which retains its complete tissue type plasminogen ( t PA ) inhibitory activity , to fibrin matrices formed in 2 cm 2 tissue culture wells . ^^^ Fibrin , pretreated with activated PAI 1 , was protected from t PA mediated plasmin degradation in a PAI 1 dose responsive manner ( IC 50 = 12 . 3 nM ) . ^^^ Clot protection correlated with partial occupancy of the low affinity PAI 1 binding site on fibrin and was due to the formation of sodium dodecyl sulfate stable , PAI 1 . t PA complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , it seems likely that elevated levels of active PAI 1 can negate the thrombolytic effects of rt PA in vivo and argues against the possibility that t PA can dissociate from PAI 1 and have its activity restored in the presence of a thrombus . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma PAI 1 activities either on admission ( 9 . 2 38 . 2 U / ml ) and after plasma exchange therapy ( 2 . 6 38 . 6 U / ml ) showed a behavior irrespective of t PA : Ag changes , and post plasmapheresis values remained high only in patients with fatal neurological outcome . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) activity , plasminogen , alpha 1 proteinase inhibitor ( alpha 1 PI ) , alpha 2 antiplasmin ( alpha 2 AP ) , antithrombin 3 ( AT 3 ) , fibrinogen , and ELT were measured 0 . 5 , 1 , 3 , and 6 h after the induction of taurocholate AEP in rats , as well as in sham operated animals and the control group , which was not submitted to any operation . ^^^ T PA activity decreased significantly after 3 and 6 h of AEP ; PAI activity had a time course reverse to t PA and was parallel to alpha 1 PI activity . ^^^ Increase of PAI activity and decrease of t PA could be a reasonable explanation for inhibited plasma euglobulin fibrinolytic activity noted in the early period of AEP . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The high affinity binding site has been identified as PA inhibitor type 1 ( PAI 1 ) , which binds to the catalytic portion of the molecule , while the second site binds t PA through an active site independent domain . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) antigen ( Ag ) and activity and tissue plasminogen activator inhibitor ( PAI ) were measured before and after DDAVP . t PA Ag was found to be significantly higher before DDAVP in patients than in controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To elucidate the circadian variation of fibrinolytic components in vasospastic angina , plasma levels of tissue plasminogen activator antigen ( t PA ) , free plasminogen activator inhibitor antigen ( free PAI 1 ) , t PA / PAI 1 complex , and total PAI 1 were measured in venous plasma samples . ^^^ All of the fibrinolytic components showed circadian variation , with a peak level at 6 : 00 AM in every study group except for the t PA / PAI 1 complex in the group of patients with exertional angina . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , the fibrinolytic activity of t PA in the supernatant was similar in the two preparations and fibrin autography demonstrated only a minor degree of t PA PAI 1 complex formation . ^^^ Furthermore , a similar platelet dependent reduction in clot lysis was seen with a t PA mutant resistant to inhibition by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) antigen and activity , plasminogen activator inhibitor ( PAI ) antigen and activity , thrombin antithrombin 3 ( TAT ) complexes were determined in blood samples . ^^^ Diabetic CAD patients showed higher TAT levels with clearly increased PAI levels whereas t PA levels levels were similar in patients and controls . ^^^ Long term defibrotide treatment induced marked changes in fibrinolytic parameters of these diabetic patients with CAD with increased t PA activity , that could be related to an evident reduction of PAI antigen and activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic capacity of the blood mainly depends on the amount of tissue plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) . ^^^ In 10 % of patients the decrease in fibrinolytic activity resulted from a lower t PA release ( `` lower ' ' was defined as mean minus one standard deviation of the control group ) . 40 % showed elevated plasma PAI capacity before VOT ( `` elevated ' ' was defined as mean plus two standard deviations of the control group ) . ^^^ Reduced fibrinolytic activity in 109 patients with coronary heart disease based either on a decrease in t PA antigen release or a increased in PAI capacity in comparison with healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was aimed to clarify the influence of aging and circadian fluctuation of fibrinolytic factors to changes of tissue plasminogen ( t PA ) and its inhibitor ( PAI 1 ) during venous occlusion test . ^^^ Plasma concentration of t PA , free PAI 1 and total PAI 1 were measured by ELISA . ^^^ Circadian variation was observed in the change of t PA activity and total PAI 1 . ^^^ However , this phenomenon was different between age groups and increase of t PA activity occurred in elderly subjects , whereas PAI 1 was observed in young subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma epinephrine , active and total t PA , active plasminogen activator inhibitor type 1 ( PAI 1 ) and t PA / PAI 1 complex concentrations were measured at each exercise and infusion level . ^^^ In these healthy subjects , active plasminogen activator inhibitor type 1 and t PA / PAI 1 complex levels were low ( 41 + / 11 and 21 + / 5 pmol / liter , respectively ) and did not change significantly during exercise or epinephrine infusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) antigen was measured in a phosphate buffered saline ( PBS ) extract of each thrombus ; t PA antigen and plasminogen antigen were determined in a 6 M urea extract of the thrombus , representing bound proteins . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) antigen was measured in a phosphate buffered saline ( PBS ) extract of each thrombus ; t PA antigen and plasminogen antigen were determined in a 6 M urea extract of the thrombus , representing bound proteins . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effect of fibrin on the interaction of human recombinant single chain tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) was studied in normal rabbit plasma and in plasma with high levels of native PAI 1 . t PA was added to diluted plasma containing calcium ( 10 mM ) and 125I fibrinogen at 37 degrees C . ^^^ The activity of t PA ( 50 IU / ml ) was rapidly reduced to 15 % of the initial value in plasma containing PAI 1 ( 23 AU / ml ) . ^^^ When thrombin and t PA were added simultaneously to the plasma , more than 70 % of the activity was retained through incorporation of t PA into the fibrin clot . t PA induced fibrinolysis in PAI 1 enriched plasma was further delayed when the temperature was reduced from 37 to 25 degrees C . ^^^ These results indicate that t PA induced fibrinolysis in PAI 1 enriched plasma is modulated by the presence of fibrin and by temperature . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We evaluated diurnal changes ( 8 : 00 am vs 4 : 00 pm ) in PAI 1 ( functional and immunological assays ) , t PA Ag and t PA / PAI 1 complex levels in controls and subjects with high PAI 1 levels . ^^^ The decrease in t PA Ag and t PA / PAI 1 complex levels was the same in controls and in obese . ^^^ Great variability in PAI 1 , t PA Ag or t PA / PAI 1 complex levels was observed in patients with acute inflammatory syndrome or infection for whom classical biorhythms are suppressed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine a possible relation of changes in plasma levels of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) to the development of coronary restenosis after successful coronary angioplasty ( PTCA ) , we followed 104 patients with a low grade residual stenosis after PTCA ( less than 30 % ) for a period of 12 months . ^^^ PAI 1 plasma levels ( functional activity ) and t PA antigen were determined 1 day before PTCA and 3 days , 3 months and 6 months thereafter . ^^^ No significant differences could be demonstrated in t PA antigen or PAI 1 activity ( plasma levels between the two groups of patients the day before PTCA ) . ^^^ During the whole observation period t PA plasma levels were not significantly different between the two groups ; however , PAI 1 plasma levels were significantly higher at 3 months and 6 months after PTCA in patients of group A ( p less than 0 . 005 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The decrease in plasminogen , t PA : Ag , alpha 2PI , PAI 1 , and PIC after physical training may result from the decreased coagulability . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In diabetic patients , elevated plasma levels of t PA and PAI 1 accompany impaired fibrinolysis . ^^^ To identify mechanisms for these abnormalities , we examined whether vascular endothelial cells exposed to high glucose upregulate t PA and PAI 1 production and whether ambient PA activity is decreased concomitantly . ^^^ In 17 cultures of human umbilical vein endothelial cells grown to confluency in 30 mM glucose , the t PA antigen released to the medium in 24 h was ( median ) 52 ng / 10 ( 6 ) cells ( range 10 384 ) and the PAI 1 antigen was 872 ng / 10 ( 6 ) cells ( range 217 2074 ) both greater ( P less than 0 . 02 ) than the amounts released by paired control cultures grown in 5 mM glucose 29 ng / 10 ( 6 ) cells ( range 7 . 5 216 ) and 461 ng / 10 ( 6 ) cells ( range 230 3215 ) , respectively . ^^^ In the presence of high glucose , the steady state levels of t PA and PAI 1 mRNAs were increased correspondingly ( median 142 and 183 % of control , respectively , P less than 0 . 05 ) ; high glucose per se and hypertonicity contributed to the upregulation in additive fashion . ^^^ Thus , high glucose coordinately upregulates endothelial t PA and PAI 1 expression through effects exerted at the pretranslational level and enhanced by even mild degrees of hypertonicity . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , we measured markers of free radical activity ( nonperoxide conjugated diene isomer of linoleic acid [ PL 9 , 11 LA ' ] and lipid peroxides expressed as malondialdehyde [ MDA ] ) along with the hemostatic variables : fibrinogen , von Willebrand factor ( vWf ) , plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( t PA ) , and plasmin activity ( B beta 15 42 ) in 24 NIDDM patients ( 12 patients with microalbuminuria and 12 without microalbuminuria ) and in 12 age matched control subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of the two activators of the fibrinolytic system : tissue and urokinase type PAs ( t PA and u PA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of the two activators of the fibrinolytic system : tissue and urokinase type PAs ( t PA and u PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
HGF did not efficiently enhance expression of both the t PA gene and a tissue inhibitor of metalloproteinase gene whereas it stimulated expression of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TGF beta 1 also caused an increase of both u PA and PAI 1 antigens , while there was no detectable effect on t PA . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Standard heparin but not LMW heparin induces a concomitant increase of t PA and PAI 1 without modification of global fibrinolysis : study after 60 days treatment . ^^^ We investigated fibrinolytic parameters ( ECLT , t PA antigen , PAI 1 activity and antigen , t PA / PAI 1 complexes ) before treatment ( D 0 ) and at D 30 and D 60 , before and after venous occlusion ( VO ) . ^^^ A significant and marked increase in t PA and t PA / PAI 1 complexes at D 30 and D 60 before and after VO was noted only in the SH group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Zymographic analysis of secreted PA related compounds revealed production of free urokinase ( u PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) complexed to tissular plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The changes of fibrinolytic activity suggest that t PA , PL and FDP were increased , while PAI , PLG , and alpha PI were decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Complexes of t PA with plasminogen activator inhibitor 1 ( PAI 1 ) , alpha 2 macroglobulin and C 1 inhibitor were also observed . ^^^ PAI 1 antigen rose dramatically in both patients , and complexes of t PA with PAI 1 rose rapidly during the period of observation . ^^^ The observations demonstrate that there is a major release of t PA in such defibrinating patients , that there is a role for protease inhibitors other than PAI 1 in the regulation of endogenous t PA , and indicate the great rapidity with which such free t PA is complexed and cleared . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Explants from a glioblastoma multiforme were maintained for 4 weeks in a three dimensional Gelfoam matrix culture in order to study the synthesis of alpha 2 antiplasmin ( alpha 2AP ) , alpha 2 macroglobulin ( alpha 2M ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , we looked for alterations of fibrinolytic parameters ( tissue plasminogen activator ( t PA ) , tissue plasminogen activator inhibitor ( PAI ) , D dimer , euglobulin clot lysis time ( ECLT ) , plasminogen , alpha 2 antiplasmin ) and of some coagulation parameters ( prothrombin time , fibrinogen , platelets , antithrombin 3 , protein C , factor 12 ) in clearly defined septic patients and for the relations of these values to the severity of the disease ( APACHE 2 score ) . ^^^ In contrast the systemic fibrinolytic capacity of the blood was strongly inhibited : t PA activity was not detectable , PAI function was elevated , the ECLT was prolonged and alpha 2 antiplasmin was normal . ^^^ The changes in t PA antigen , PAI function , factor 12 , prothrombin time and antithrombin 3 were significantly related to the APACHE 2 score of the patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The second order rate constants for t PA inhibition of `` wild type ' ' PAI 1 and PAI P 1 P1 ' ATIII , irrespective of the presence of vitronectin , were similar , whereas replacing P 3 P3 ' resulted in a 40 fold decrease of the second order rate constant towards t PA , again independent of vitronectin . ^^^ Thus , in the presence of vitronectin , PAI 1 P 3 P3 ' ATIII displays a 3 fold higher k 1 with thrombin than with t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Whereas PAI 1 production increased , t PA production by human endothelial cells in vitro remains unaltered or even decreases on TNF addition . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We found significantly higher levels of thrombin antithrombin 3 ( TAT ) complexes , lower levels of systemic tissue plasminogen activator ( t PA ) activity , and higher levels of plasminogen activator inhibitor ( PAI ) activity in the AMI patients compared to the UAP patients . ^^^ Studies of the relationship between coagulation ( TAT complexes ) and fibrinolysis data revealed a significant positive correlation between plasma antigen concentrations of TAT complexes and t PA ( P less than 0 . 02 ) , and between TAT complexes and PAI 1 ( P less than 0 . 002 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The authors examined the effect of insulin like growth factor 1 ( IGF 1 ) , epidermal growth factor ( EGF ) , and acidic fibroblast growth factor ( AFGF ) on the synthesis by human retinal endothelial cell ( HREC ) of plasminogen activators ( PA ; tissue type [ t PA ] and urokinase type [ u PA ] ) and plasminogen activator inhibitor ( PAI ) . ^^^ Immunologic and functional assays for t PA , u PA , and PA 1 were conducted with cell lines derived from three diabetics and three nondiabetic controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before injection , almost all patients had elevated D dimer , t PA and PAI 1 levels . ^^^ Immediately after the injection , TAT , D dimer , and t PA levels rose significantly ( P less than 0 . 001 , P less than 0 . 01 , P less than 0 . 001 ) , PAI 1 and PC levels decreased ( P less than 0 . 01 ) , while antithrombin , alpha 2 antiplasmin , and fibrinogen concentrations were unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 PAI ( PAI 1 ) is the physiological inhibitor both of urinary type PA ( u PA ) and tissue type PA ( t PA ) ( Loskutoff et al . , 1989 ) and is a major component of the ECM of cultured cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , an essential regulatory protein of the fibrinolytic system , harbors interaction sites for plasminogen activators ( tissue type [ t PA ] and urokinase type [ u PA ] ) and for fibrin . ^^^ MoAbs , binding to two distinct areas of PAI 1 , are able to prevent the inhibition of t PA by PAI 1 . ^^^ The area located between amino acids 110 and 145 of PAI 1 contains a binding site for both components and its significance is discussed in the context of the t PA inhibition by fibrin bound PAI 1 . ^^^ We propose that , upon activation of platelets , PAI 1 is fixed within the clot by binding to fibrin and retains its full capacity to inhibit t PA and u PA . . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , an essential regulatory protein of the fibrinolytic system , harbors interaction sites for plasminogen activators ( tissue type [ t PA ] and urokinase type [ u PA ] ) and for fibrin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the fast acting inhibitor of both tissue type and urokinase type plasminogen activators ( t PA , u PA ) and is an essential regulatory protein of the fibrinolytic system . ^^^ To assess whether these cofactors turn PAI 1 into a general protease inhibitor or whether their influence is restricted to thrombin , the second order association rate constants between PAI 1 and the human plasma proteases t PA , u PA , plasmin , thrombin , Factor Xa ( FXa ) , and Factor XIIa ( FXIIa ) in the absence and in the presence of either vitronectin or heparin are determined . ^^^ In addition , vitronectin moderately increases the rate of inhibition by PAI 1 of u PA and of plasmin , but does not alter the rate of inhibition of t PA , FXa , or FXIIa ; ( 3 ) Apart from the important role of the P 1 residue , no consensus can be presented on the nature of other residues within the P 3 to P 3 ' region with regard to target protease specificity . . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the fast acting inhibitor of both tissue type and urokinase type plasminogen activators ( t PA , u PA ) and is an essential regulatory protein of the fibrinolytic system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We propose that the hemostatic molecular markers TAT , D dimer , and PIC are suitable for the early diagnosis of DIC after endoscopic embolization using thrombin in patients with liver cirrhosis and that the increase of PAI 1 is too small for the regulation of fibrinolysis due to t PA in DIC occurring in liver cirrhosis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic system components like tissue plasminogen activators ( t PA ) , and urokinase ( u PA ) , and their inhibitors PAI 1 and PAI 2 were all studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Vitronectin ( VN ) stabilizes plasminogen activator inhibitor type 1 ( PAI 1 ) activity and prevents the fibrin ( ogen ) induced acceleration of plasminogen activation by t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relevance of PAI and alpha 2AP for clot lysis was considered in relation to data in the literature concerning the behaviour of t PA and factor XIII . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total fibrinolytic activity did not change during the treatment but the antigens of two of the major components of the fibrinolytic system , i . e . t PA and PAI 1 , were significantly reduced at the end of the study . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A computer simulation was developed to study the regulation of active tissue plasminogen activator ( t PA ) levels in plasma by kinetically modeling t PA secretion , t PA inhibition by plasminogen activator inhibitor type 1 ( PAI 1 ) , and hepatic clearance of t PA , PAI 1 and t PA / PAI 1 complex throughout a simplified human circulatory system . ^^^ The model indicates that as the active PAI 1 concentration increases , the percent of t PA in the active form decreases exponentially . ^^^ Further , the reaction between t PA and PAI 1 substantially reduces the half lives of both active factors . ^^^ By adjusting the t PA and PAI 1 secretion rates to provide the best fit between simulated and measured circadian variations in t PA , PAI 1 and complex , the model predicts that the diurnal rhythm in active t PA levels is principally due to changes in the rate of PAI 1 secretion and not to variations in the t PA secretion rate . ^^^ In conclusion , the model predicts that PAI 1 is an important regulator of the concentration , half life and circadian variation of active t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) levels in HOTMEC conditioned medium were 50 fold higher than those of HUVEC , and the PAI 1 secretions were identical in both cell types . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , the values of other fibrinolytic components , such as t PA antigen , t PA / PAI 1 complex and free PAI 1 antigen , in the controls and patients were similar . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of VEGF , a recently described angiogenic factor , were assessed on PA activity and PA and PAI 1 mRNA levels in microvascular endothelial cells . u PA and t PA activity were increased by VEGF in a dose dependent manner , with maximal induction at 30 ng / ml . u PA and t PA mRNAs were increased 7 . 5 and 8 fold respectively after 15 hours , and PAI 1 mRNA 4 . 5 fold after 4 hours exposure to VEGF . ^^^ At equimolar concentrations ( 0 . 5 nM ) , VEGF was a more potent inducer of t PA mRNA than bFGF , while bFGF was a more potent inducer of u PA and PAI 1 mRNAs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At the same time ( 4th 7th day ) the plasminogen activator activity ( PAA ) in seminal plasma was found to be increased , but the plasminogen activator inhibition ( PAI ) expressed against t PA ( anti t PA ) showed an increase between 32nd and 46th day . ^^^ In spermatozoa a marked increase of PAA was revealed between 32nd and 46th day post injection , while an increase of PAI ( anti t PA ) was exhibited on the 74th day . ^^^ A positive correlation has been found between increased concentrations of testosterone and PAA or PAI ( anti t PA ) in spermatozoa and seminal plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A special attention has been focused in changes in the levels of tissue plasminogen activator ( t PA ) activity and antigen and plasminogen activator inhibitor 1 ( PAI 1 ) as indexes of fibrinolysis capacity , representing parameters of vascular endothelial functions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The ratio of t PA / PAI 1 ( 10 100 ) , calculated as index for the endogenous fibrinolytic activity increased significantly from 8 . 1 + / 1 . 1 to 14 . 5 + / 1 . 6 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Reviewed are studies that indicate that it is possible in patients with diabetes by means of sulfonylurea compounds to increase endothelial cell produced t PA without affecting PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activators t PA , u PA and its inhibitor ( PAI ) in normal males and females . ^^^ We determined the plasma levels of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) activity and their antigen levels including urokinase plasminogen activator ( u PA ) in 33 male and 27 female normal subjects . ^^^ The respective mean levels of t PA and PAI antigen were significantly higher ( p less than 0 . 01 ) in males ( 8 . 1 ng / ml and 17 . 6 ng / ml ) than in females ( 6 . 2 ng / ml and 12 . 1 ng / ml ) . ^^^ This study demonstrates that significant differences in t PA , u PA and PAI exist between male and female subjects which should be taken into account when determining their levels in clinical conditions . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Dynamic change of serum tissue type plasminogen , activator ( t PA ) and PAI activity was studied in 22 cases with acute myocardial infarction ( AMI ) 11 cases with unstable angina ( UA ) and 24 healthy persons . ^^^ The results revealed : t PA activity was decreased in AMI group , especially in AMI A subgroup ; PAI activity was markedly increased in AMI A subgroup in first three days after onset ; there was a marked decrease of active type in AMI A subgroup . ^^^ The change of activity of t PA and PAI is closely related with the severity of AMI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Ethanol at both concentrations induced after 30 days a decreased PAI in the pyloric region and the body of the stomach , which was expressed against u PA , but not against t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A complete fibrinolytic profile including total fibrinolytic activity ( TFA ) , tissue plasminogen activator ( t PA ) , plasmin inhibitor ( PI ) , plasminogen activator inhibitor ( PAI ) , protein C ( PC ) and plasminogen ( PLG ) was obtained preoperatively in 114 brain tumor patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The activation of plasminogen by T PA PAI 1 complex in the absence of fibrin . ^^^ The activity of tissue plasminogen activator plasminogen activator inhibitor 1 ( t PA PAI 1 ) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer . ^^^ Fibrin autography was more sensitive to the activity of t PA even in the complex with PAI 1 , but casein zymography was more sensitive to plasmin . ^^^ The inhibitory activity of PAI 1 toward t PA diminished when plasma was clotted , thus in the presence of fibrin . ^^^ These results indicate that t PA PAI 1 complex has a catalytic activity even in the absence of fibrin , but the activity is enhanced in its presence . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The receptor for tissue plasminogen activator ( t PA ) in complex with its inhibitor , PAI 1 , on human hepatocytes . ^^^ The binding of t PA PAI 1 to human hepatocytes at 4 degrees C reached a maximum at 2 h . ^^^ Binding of [ 125I ] t PA PAI 1 complex was unaffected by high concentrations of unlabelled t PA , PAI 1 , u PA or u PA PAI 1 complex ; only t PA PAI 1 complex competed for binding . ^^^ Hepatocyte bound t PA PAI 1 was internalized and degraded at 37 degrees C . ^^^ Thus , hepatocytes have a specific t PA PAI 1 receptor that participates in clearance of this complex . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We analyzed the effect of IL 1 on the synthesis of collagenase , stromelysin , and tissue inhibitor of metalloproteases ( TIMP ) in human cartilage explants and culture chondrocytes , as well as its effect on the secretion of plasminogen activators ( t PA , u PA ) and inhibitors ( PAI 1 , PAI 2 ) in cartilage explants . ^^^ The increase in t PA synthesis was accompanied by a decreased PAI 1 level , while the PAI 2 level remained unchanged . u PA could not be detected in the culture medium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following fibrinolytic variables were analysed in plasma and related to thromboembolism : tissue plasminogen activator ( t PA ) activity , t PA antigen ( t PA Ag ) , plasminogen activator inhibitor ( PAI 1 ) activity and PAI 1 antigen ( PAI 1 Ag ) . ^^^ Immediately after surgery and in the morning the first postoperative day the levels of PAI 1 activity , PAI 1 Ag and t PA Ag were positively correlated to thromboembolism . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Stimulation by EGF of cell migration of HME cells and its inhibition by TNF appear to be closely correlated with the cellular modulation of t PA and PAI 1 activities . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relative molecular masses of these species ( 60 to 80 and 110 Kd ) suggest that they represent recombinant human t PA both free and bound to sheep plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Clearance of t PA , PAI 1 , and t PA PAI 1 complex in an isolated perfused rat liver system . ^^^ In this study , the clearance of t PA , plasminogen activator inhibitor 1 ( PAI 1 ) , and t PA PAI 1 complex was determined in an isolated perfused rat liver system . ^^^ The clearance of t PA PAI 1 complex was twice as fast as that of t PA , whereas PAI 1 was cleared slowly . ^^^ The corresponding values for t PA PAI 1 complex were : alpha , 9 . 7 minutes , and beta , about 7 hours . ^^^ The model microconstants were computed for t PA and t PA PAI 1 complex and the marked difference between the `` on ' ' microconstants k 12 for t PA ( 0 . 026 + / 0 . 001 min 1 ) and t PA PAI 1 complex ( 0 . 090 + / 0 . 025 min 1 ) suggests that the effect on binding to liver cells is the most important factor in the faster clearance of t PA PAI 1 complex when compared with t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) activity increased ( P less than 0 . 02 ) , and t PA antigen decreased ( P less than 0 . 01 ) , whereas plasminogen activator inhibitor ( PAI 1 ) and fibrinogen were unaffected by metformin treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and its inhibitor ( PAI ) were assessed in venous blood drawn before and after venous occlusion ( bvo , avo ) for 33 patients with Raynaud ' s phenomenon ( RP ) , 14 with primary RP ( PRP ) , 9 with suspected secondary RP ( SSRP ) , and 10 with definite collagen disease and secondary RP ( SRP ) . ^^^ PAI activity decreased significantly avo only in controls and in PRP , and there was significant t PA antigen elevation avo in the same groups . ^^^ In conclusion , there is an endothelial derangement , involving t PA release and PAI , in SSRP and SRP patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PA inhibitor type 1 ( PAI 1 ) antigen , PAI activity , and t PA activity of the euglobulin fraction of plasma . ^^^ However , the fish diet was concurrently associated with the putative unfavourable higher levels of PAI 1 antigen ( 3 . 0 vs . 1 . 2 ng ml 1 ) and PAI activity ( 6 . 1 vs . 3 . 2 IU ml 1 ) , and lower t PA activity ( 80 vs . 140 mIU ml 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , high baseline plasma PAI 1 levels may be detrimental to reperfusion with t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was performed to elucidate the acute effect of insulin on levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor of endothelial cell type ( PAI 1 ) . ^^^ The t PA activity gradually increased ( 75 % mean increase after 2 h , p less than 0 . 001 ) and the PAI 1 activity decreased ( 49 % mean decrease after 2 h , p less than 0 . 001 ) during the clamp . t PA activity decreased and PAI 1 activity increased after the insulin infusion was ceased , but they were still 48 % higher and 38 % lower , respectively , after 60 min . ^^^ PAI 1 and t PA activities were not affected by saline infusion for 2 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lowering of PAI activity was found during the acute phase , which was reversed during the recovery phase and remained significantly elevated during the sequelar stage compared with the controls ( P less than 0 . 001 ) , F test showed that both t PA and PAI activities changed significantly during various stages ( t PA , P less than 0 . 01 ; PAI , P less than 0 . 001 ) . ^^^ Because of these changes , the ratio of PAI and t PA fluctuated during different stages of the disease . ^^^ The ratio , however , elevated abnormally during the sequelar stage , compared with healthy controls ( P less than 0 . 05 ) , In addition , the result of correlation test and linear regression analysis of serum t PA and PAI activities in 54 cases showed a significant negative correlation ( P less than 0 . 001 ) existed between t PA and PAI activities . ^^^ The balance of plasma t PA and PAI activities have the function in maintaining the normal bloodstream in human body . ^^^ In the process of disease , the abnormality of plasma t PA and PAI activities may be the different pathological characteristics involved in this stagnation process . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Determination of t PA and PAI 1 : recommendations for the collection of samples ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In Type 1 diabetic patients , after 2 3 months of treatment with gliclazide , we observed a significant increase in plasma concentrations of total t PA antigen that remained stable until discontinuation of the drug ( p less than 0 . 0002 ) , whereas the plasma concentrations of plasminogen activator inhibitor ( PAI ) did not change significantly during the study . ^^^ Moreover , the plasma concentrations of total t PA antigen increased significantly ( p less than 0 . 02 ) in this group of diabetic patients while PAI remained unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
U PA was determined by a competition RIA , t PA by an ELISA and PAI by a spectrophotometric assay . 15 patients showed normozoospermia , 11 azoospermia and 41 oligoasthenoteratozoospermia ( OAT syndrome ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Evaluation of a new enzyme immunoassay method for determination of t PA PAI 1 complex ] . ^^^ We evaluated a new enzyme immunoassay for determination of t PA PAI 1 complex ( PAI C ) and studied the clinical utility of measuring PAI C . ^^^ This assay was performed by the capture / tag antibody technique using polystylene beads , in which the beads were coated with monoclonal antibody against PAI 1 and anti t PA polyclonal antibody was tagged ( TDC 88 , TEIJIN LIMITED , Japan ) . ^^^ In patients with accelerated fibrinolysis , PAI C values correlated with t PA antigen ( r = 0 . 838 ) , PAI 1 antigen ( r = 0 . 519 ) , ATIII activity ( r = 0 . 669 ) ( p less than 0 . 01 ) and D dimer levels ( r = 0 . 391 , p less than 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Basal values of plasma tissue plasminogen activator ( t PA ) activity and antigen and of t PA inhibitor ( PAI ) were normally distributed in this patient group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in alpha 2 PI activity and the levels of fibrinogen , t PA and PAI antigen were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These findings strongly suggest that acute ischemia initiates systemic induction of excessive and continuous release of t PA , which outweighs any anticipated increase in PAI activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following fibrinolytic variables were analysed in plasma and related to the different steps of surgery : tissue plasminogen activator ( t PA ) activity , t PA antigen and plasminogen activator inhibitor ( PAI 1 ) activity . ^^^ During surgery PAI 1 activity and t PA antigen in the operated limb were significantly increased compared with preoperative values . ^^^ There was a significant difference in PAI 1 activity and t PA antigen between the operated and the non operated limbs during surgery and within one hour postoperatively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cultured bovine lens epithelial cells released tissue plasminogen activator ( t PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) . t PA activity was completely inhibited by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Chem . 28 , 870 873 ) to quantitate ionic surfactants , and a discontinuous spectrophotometric assay for the titration of PAI 1 based on the measurement of residual active t PA , we found ( 1 ) that SDS remains tightly bound to PAI 1 after equilibrium dialysis and ( 2 ) that the activity of the latter was closely related to the amount of SDS carried over by the PAI 1 solution . ^^^ The highest concentrations of SDS ( ratio of SDS to protein greater than 0 . 1 ) were detected in the platelet derived sources of PAI 1 which also showed the lowest residual t PA activity . ^^^ Moreover , it is demonstrated by SDS PAGE and autoradiography that the tight binding of SDS to PAI 1 decreases its ability to form complexes with t PA . ^^^ Similar results were obtained with PAI 1 previously inactivated at 37 degrees C : the inability of PAI 1 to form complexes with t PA was unchanged after SDS treatment . ^^^ These observations suggest that the decrease in the residual activity of t PA observed with the SDS treated PAI 1 preparations is not related to an increase in the inhibitory activity of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Unlike endothelial cells where plasminogen activator inhibitor 1 has been implicated in mediating a high affinity interaction of t PA with the cells , no evidence for a role of this inhibitor in ligand binding to the monocytoid cells was found . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Defibrotide is a polydeoxyribonucleotide drug known to modulate the endothelial cell release of t PA , PAI , and PGI 2 and to improve blood flow and perfusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
ICI 200 , 880 administration increased elastase inhibitory activity without affecting t PA and plasminogen activator inhibitor ( PAI 1 ) activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was undertaken to investigate whether beta blockade alters the circadian rhythm of 2 major fibrinolytic factors , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Blood samples were analyzed for the plasma levels of t PA activity , t PA antigen , PAI activity and PAI 1 antigen . ^^^ The data therefore suggest that propranolol treatment does not affect the plasma concentrations at rest or the endogenous circadian rhythm of t PA and PAI 1 in healthy volunteers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recurrent postoperative bleeding in a previously healthy man with a moderate prolongation of PT and PTT which did not correct with vigorous fresh frozen plasma infusion was identified as a unique abnormality of the fibrinolytic system : a combination of an excess of tissue plasminogen activator ( t PA ) at 300 % of normal activity accompanied by a concurrent deficiency of plasminogen activator inhibitor 1 activity ( PAI 1 ) of 10 20 % of normal activity . ^^^ This observation underscores the potentially important regulatory role of PAI 1 in the degree to which clinical manifestations of bleeding tendencies may occur in patients in whom an excess amount of t PA is expressed . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , the authors determined the activity and antigen levels of u PA and t PA , and their inhibitors , plasminogen activator inhibitors types 1 and 2 ( PAI 1 and PAI 2 ) , in normal mucosa , adenomatous polyps , and adenocarcinomas of the human colon . ^^^ The decrease in t PA activity in the neoplastic tissues , determined enzymatically and zymographically , was significantly correlated with an increase in PAI 1 and PAI 2 , in particular in carcinomas . ^^^ The authors conclude that PAI 1 and PAI 2 are significantly increased in neoplastic tissue of the human colon and contribute considerably to the decrease of t PA activity in carcinomas . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Familial clustering of defective release of t PA . 91 unrelated patients with idiopathic or familial deep vein thrombosis ( DVT ) and 72 ( 34 with DVT ) relatives from 26 families were screened for hypofibrinolysis by measuring tissue plasminogen activator antigen ( t PA : Ag ) after venous occlusion ( VO ) for 10 and 20 min and by measuring t PA inhibitor activity ( PAI ) at rest . 21 healthy subjects served as controls . ^^^ In contrast to the defective release of t PA : Ag , increased basal level of PAI did not show familial clustering . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand , TNF up regulates the production of procoagulant factors such as t PA inhibitor ( PAI ) , tissue factor and platelet activating factor ( PAF ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) rapidly inactivates tissue plasminogen activator ( t PA ) and urokinase ( UK ) with nearly identical association rate constants . ^^^ The contributions of Ser 344 , Ala 345 , and Arg 346 ( P 3 , P 2 , and P 1 residues , respectively ) in PAI 1 to inhibition of UK and t PA were evaluated using combinatorial mutagenesis of the human PAI 1 cDNA . ^^^ Each of the unique active PAI 1 were assayed for inhibitory activity towards UK or t PA ; many substitutions differentially affected the ability of the inhibitor to inactivate UK and t PA . ^^^ For example , replacement of Ser 344 and Ala 344 with Val and Pro , respectively , yielded a PAI 1 variant exhibiting an association rate constant that was unchanged for t PA but decreased 23 fold for UK , relative to native PAI 1 . ^^^ In general , the PAI 1 variants were more potent inhibitors of t PA than UK . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , the lower level of t PA activity after venous occlusion together with the higher levels of 8 : C , 8 R : Ag , alpha 2PI , PAI 1 , and plasmin . alpha 2PI complex before venous occlusion in the patients , indicated that the patient group was in a hypercoagulable and hypofibrinolytic state . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
While t PA and u PA levels were slightly lower than in adult controls , a significant decrease in PAI activity was demonstrated and no PAI 2 could be detected in fetal plasma . ^^^ In contrast with these findings , the fibrinolytic equilibrium of pregnant women exhibited a prolonged ECLT and a strong increase in both PAI activity and PAI 2 antigen levels , while only a moderate elevation in u PA and t PA levels was measured . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , since neither t PA increase nor PAI decrease was observed , we suggest that the profibrinolytic response to defibrotide may be due to mechanisms other than t PA stimulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The antigen of t PA and its inhibitor PAI , free and total , and the biologic activity of PAI were assayed in basal conditions and after treatment . ^^^ Although a marked increase of t PA was seen with the active treatment , PAI activity was significantly reduced by defibrotide . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Selective assay methods show that almost invariably the fibrinolytic activity of these patients is depressed either following increased levels of fibrinolytic inhibitors ( mainly plasminogen activator inhibitor 1 or PAI 1 ) and / or decreased levels of a plasminogen activator ( tissue plasminogen activator or t PA ) . ^^^ Thus two levels of t PA / increased levels of PAI 1 have been found in obesity , diabetes mellitus , postoperative states , SLE , malignancies , and miscellaneous diseases often complicated with thrombosis such as Behet ' s syndrome . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Defibrotide , a deoxypolyribonuclide , has been found to modulate endothelial cell function causing increase in t PA and decrease in PAI levels and also increase in PGI 2 production . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of free plasminogen activator inhibitor 1 ( PAI 1 ) and complex of t PA PAI 1 decreased from 9 : 30 h to 10 : 00 h ( p less than 0 . 02 ) and to 12 : 30 h ( p less than 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase ( u PA ) and tissue type ( t PA ) and their inhibitors PAI 1 and PAI 2 was studied . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here we report that most of the specific binding of 125I t PA to our HUVEC cultures is accounted for by binding to ( 1 ) plasminogen activator inhibitor type 1 ( PAI 1 ) , a t PA inhibitor produced in abundance by HUVECs ; and ( 2 ) specific binding sites present on the plastic culture surface . ^^^ Interestingly , a radioiodinated mutant form of t PA , S478A , which is catalytically inactive and therefore unable to form the covalent complex with PAI 1 , still binds to HUVECs . ^^^ Several findings suggest that PAI 1 is a major binding site for S478A t PA . ^^^ It is proposed that t PA releases PAI 1 from the solid phase when it forms the irreversible covalent complex with the inhibitor , a process that does not occur with the catalytically inactive mutant form of t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) was identified in extracts of Lewis lung carcinoma , and its immunohistochemical localization was studied together with that of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators . ^^^ Lung metastases always contained u PA immunoreactivity , while PAI 1 immunoreactivity was found in most , but not all , metastases . t PA immunoreactivity was found in a few scattered tumor cells , in primary carcinomas as well as metastases . ^^^ Controls that included absorption with highly purified antigen preparations and immunoblotting , indicated that all the immunoreactivity represented genuine PAI 1 , u PA and t PA , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following determinations were carried out on venous blood : t PA antigen ( ELISA ) , t PA activity , and t PA inhibitor ( PAI ) activity ( amidolytic assays ) . ^^^ The poor fibrinolytic response of leg vessels to venous occlusion is not due to a high PAI release or t PA stores depletion in leg vessels , but rather to low basal t PA release in leg vessels . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate possible hormonal regulators of the diurnal rhythm in fibrinolytic activity , we measured tissue plasminogen activator ( t PA ) activity , plasminogen activator inhibitor activity ( PAI 1 ) , t PA antigen , insulin , cortisol , and catecholamines in 6 healthy males ( age 34 + / 5 ) every 2 hours for 24 hours . ^^^ Fibrinolysis was characterized by a peak in PAI 1 activity and a trough in t PA activity at 0600 h . ^^^ PAI 1 activity increased 92 % and t PA activity decreased 56 % between 2400 h and 0600 h . t PA antigen ( principally a measure of t PA / PAI 1 complex ) , peaked at 0800 h . ^^^ While cortisol demonstrated the expected circadian change , the increase in cortisol did not occur until 0400 h , 4 6 hours after the rise in PAI 1 and decrease in t PA activity started . ^^^ From this data , we hypothesize that the increased level of PAI 1 in the morning consumes t PA , leading to decreased t PA activity and increased t PA / PAI 1 complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Species of PAs and PAI secreted from the GECs were urokinase type PA ( u PA ) and tissue type PA ( t PA ) , while the major species was a single chain u PA in the amount of 28 . 6 + / 2 . 34 ng / 10 ( 5 ) cells for 24 hours ( N = 4 , mean + / SD ) , and PAI 1 . ^^^ The addition of increased concentrations of thrombin ( 0 . 1 to 31 . 6 U / ml ) into confluent cultures enhanced the GECs to release u PA , t PA and PAI 1 in a dose and time dependent manner . ^^^ The incubation of the GECs with 10 U / ml thrombin resulted in about a fourfold increase in the concentration of u PA , threefold in t PA and twofold in PAI 1 . ^^^ IL 1 enhanced the release of t PA and PAI 1 , and TNF did that of u PA and t PA , while gamma IFN showed no significant effects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although less prominent , t PA and a 48 kDa form of u PA follow a similar pattern of induction and inhibition ; ( 2 ) changes in u PA activity in response to castration and cortisol treatment are due to alterations in the level of u PA mRNA and protein rather than in the activity of PAI 1 ; ( 3 ) not all castration induced proteinases in the prostate are inhibited by cortisol . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , the t PA kringle 2 domain is important for the interaction of t PA not only with fibrin , but also with plasminogen activator inhibitor 1 and thus represents a key structure in the regulation of fibrinolysis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of PAI 1 levels on the molar concentrations of active tissue plasminogen activator ( t PA ) and t PA / PAI 1 complex in plasma . ^^^ We determined the in vivo molar concentrations of active tissue plasminogen activator ( t PA ) , active plasminogen activator inhibitor type 1 ( PAI 1 ) , and t PA / PAI 1 complex . t PA activity was measured in plasma stabilized by immediate acidification . ^^^ PAI 1 activity and t PA / PAI 1 complex antigen were measured in citrated plasma ; these measurements were corrected for the loss in PAI 1 activity and increase in complex that occurs in unacidified plasma samples due to the continued reaction between t PA and PAI 1 after the sample was drawn . ^^^ To convert t PA and PAI 1 activity measurements into molar concentrations we determined the specific molar activity of t PA and PAI 1 in vivo : 4 . 48 10 10 ( 13 ) IU / mol . ^^^ Of 72 subjects studied , 13 had less than 150 pmol / L active PAI 1 ; in these individuals 33 % + / 21 % of their t PA was active and the molar ratio of active t PA to active PAI 1 was 0 . 20 + / 0 . 13 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The tissue specific distribution of tissue type and urokinase type plasminogen activator ( t PA and u PA ) and their inhibitor type 1 ( PAI 1 ) was analyzed at mRNA level in five major rat organ tissues . t PA mRNA was detected in lung , kidney , heart , and liver . u PA mRNA was detected in kidney and lung . ^^^ Endotoxin injection also caused an increased level of t PA mRNA in heart and kidney , and an increased u PA mRNA level in kidney . mRNA analysis of freshly isolated and separated subfractionated liver cells showed that the marked increase in PAI 1 mRNA in the liver after endotoxin injection may be due mainly to a strong increase of PAI 1 mRNA in the liver endothelial cells . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin clot lysis time , fibrin plate , tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor ( PAI ) activity , Protein C and S , cholesterol , triglycerides and Hb A1c were determined in blood samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This study was designed to investigate t PA activity , t PA antigen and plasminogen activator inhibitor 1 ( PAI 1 ) antigen levels in the CI patients . ^^^ The mean t PA activity , t PA antigen and PAI 1 levels in patients were 0 . 53 + / 0 . 64 IU / ml , 10 . 9 + / 5 . 2 ng / ml and 23 . 0 + / 14 . 9 ng / ml , and the normal ranges were 0 . 02 0 . 28 IU / ml , 1 . 0 7 . 0 ng / ml and 5 . 3 45 . 0 ng / ml , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To exclude age dependent or pain related influences , age matched patients with stable angina pectoris ( NHYA 2 ) and angiographically proven coronary artery disease ( CAD , n = 16 ) or without evidence for coronary sclerosis ( variant angina , n = 10 ; angina like syndrome with normal coronary angiogram , n = 5 ; non CAD , n = 15 ) have been investigated for their plasma PAI 1 activity and t PA antigen levels . ^^^ In the CAD patients no significant variation in plasma PAI 1 values could be demonstrated when related to the extent of the disease or to a history of previous myocardial infarction . t PA antigen was also elevated in CAD patients as compared to the non CAD group ( p less than 0 . 02 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of mental stress ( a colour word conflict test , CWT ) and adrenaline infusions ( 0 . 1 and 0 . 4 nmol kg 1 min 1 ) on t PA activity , t PA antigen , PAI 1 activity and PAI 1 antigen were studied in 18 healthy male volunteers . ^^^ Low and high dose adrenaline infusions yielding plasma adrenaline levels of 0 . 9 + / 0 . 1 and 3 . 4 + / 0 . 4 nmol / l , respectively , dose dependently increased t PA levels with a concomitant decrease in PAI 1 levels . ^^^ Metoprolol treatment did not influence the resting levels of the fibrinolytic variables studied , but tended to enhance the t PA response to CWT and further reduce PAI 1 during adrenaline infusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied 12 patients with respect to releasable t PA , fast acting inhibitor to t PA ( or PAI 1 ) , and immunoreactive or antigenic t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Comparatively high concentrations of HC 2 significantly suppressed thrombin stimulation of t PA : Ag and PAI 1 : Ag release but did not cause an obvious change of both release in the absence of thrombin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in plasma levels of t PA , PAI 1 and t PA PAI 1 complex in patients with cerebrovascular thrombosis infused with t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) and the in vitro ability of platelets to aggregate and of monocytes to express procoagulant ( tissue factor ) activity ( PCA ) were evaluated in five patients who are homozygous for familial hypercholesterolemia ( FH ) before and after a single and a regular 5 month cholesterol removal by low density lipoprotein ( LDL ) apheresis . ^^^ The basal levels of t PA antigen and fibrinolytic activity before and after 10 minutes of venous stasis , basal PAI activity , and PAI 1 antigen were comparable to controls and were not affected by LDL apheresis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have previously demonstrated that mutation of arginine 304 of t PA to a glutamic acid residue drastically reduces the rate of interaction between the enzyme and its suicide substrate , PAI 1 , without affecting the reactivity of the enzyme toward its normal substrate , plasminogen ( Madison , E . ^^^ We report here the use of protein modeling to design a compensatory mutation in PAI 1 ( glutamic acid 350 to arginine ) and create a molecule that rapidly inhibits this `` serpin resistant ' ' variant of t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , PAI activity , measured using single chain t PA , was lower in amniotic fluid than in plasma of normal pregnant women . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The statistical association between tissue plasminogen activator ( t PA ) activity , antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and lipids levels in blood samples used for medical check ups in 111 volunteers attending hospital for health examinations was examined . ^^^ The t PA activity level of 111 volunteers was 0 . 08 0 . 56 ng / ml ( 5 % trimmed range ) ( normal range : 0 . 08 0 . 70 ng / ml ) ; t PA antigen concentrations were 2 . 4 9 . 2 ng / ml ( normal range : 1 . 0 7 . 0 ng / ml ) ; PAI 1 antigen concentrations were 5 . 2 57 . 4 ng / ml ( normal range : 5 . 2 45 . 0 ng / ml ) . ^^^ These results show that t PA activity , antigen and PAI 1 antigen in blood obtained from the healthy volunteers are influenced significantly by the concentrations of lipids in blood . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have investigated the effect of sodium nitroprusside ( NP ) and glyceryl trinitrate ( GTN ) on fibrinolysis in anaesthetized rabbits ex vivo and in vitro by measurement of euglobulin clot lysis time ( ECLT ) , plasma levels of tissue plasminogen activator ( t PA ) activity , plasma t PA antigen levels and plasminogen activator inhibitor ( PAI 1 ) activity . 2 . ^^^ In whole blood in vitro , NP ( 30 micrograms kg 1 ) prevented the time dependent increase in PAI 1 activity and inhibited inactivation of added t PA ( 10 ng ml 1 ) . 6 . ^^^ We propose that NP exhibited fibrinolytic activity through increased t PA activity as a result of inhibition of PAI 1 release from platelets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Correlations between t PA and PAI 1 antigen and activity and t PA / PAI 1 complexes in plasma of control subjects and of patients with increased t PA or PAI 1 levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As a result , the ratio of PAI / t PA fluctuated during different stages of the disease . ^^^ Significant elevation of PAI and PAI / t PA ratio during sequelae stage may be one of the risk factors of further thrombosis and contribute partly to the high relapsing rate of the disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of molsidomine on t PA and PAI activity in man : a double blind , placebo controlled study . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Beta thromboglobulin ( BTG ) , platelet factor 4 ( PF 4 ) , fibrinopeptide A , tissue plasminogen activator ( t PA ) activity and antigen , tissue plasminogen activator inhibitor ( PAI ) , fibrin , and fibrinogen degradation products were studied during dialysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These complexes were partially dissociated with nucleophilic agents into two t PA like forms and a 52 , 000 dalton PAI protein with a pI of 4 . 8 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 , the natural inhibitor of t PA , also has Arg and Ala residues at positions P 1 and P 2 . ^^^ In a purified system , alpha 1 antitrypsin Ala 357 Arg358 was 150 fold less efficient against C1s than C 1 inhibitor and 27 , 000 fold less efficient against t PA than plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We found an intraperitoneal fibrinogen cleavage and thrombin antithrombin 3 complex formation leading to increased levels of fibrinopeptide A ( FPA : 33 . 3 + / 7 . 0 ng / ml ) and thrombin antithrombin 3 complex ( TAT : 4 . 7 + / 0 . 4 ng / ml ) in plasma by mass transfer from dialysate to plasma . t PA ( tissue plasminogen activator ) and PAI 1 ( plasminogen activator inhibitor type 1 ) concentrations in plasma were within the normal range . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were no differences in euglobulin lysis time ( ELT ) , tissue plasminogen activator ( t PA ) antigen , and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen between the study group and the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Monocyte associated PAI 1 formed complexes with single chain t PA with a molecular mass 50 kDa higher than the molecular mass of the free PAI 1 . ^^^ However , a significant amount of PAI 1 remained unbound to t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It formed SDS stable complexes with both t PA and u PA but not with prourokinase as demonstrated by both fibrin zymography and immunoblotting using anti PA and anti PAI 2 antisera after SDS PAGE . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant influence on fibrinolysis as measured by t PA and PAI assays was noticed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients with unstable coronary artery disease were randomly treated either with a combination therapy consisting of nitrates and calcium channel blockers without or with addition of clinical grade heparin administered subcutaneously ; in order to evaluate the effect of heparin treatment on the fibrinolytic system , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) plasma levels were related to the clinical course of the disease . ^^^ Heparinization led to a significant increase in t PA antigen plasma levels ( p less than 0 . 0001 ) within approximately four hours while PAI 1 activities remained unaltered . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , the levels of t PA : Ag , t PA activity , t PA inhibitor activity ( PAI ) , the von Willebrand factor ( vWF : Ag ) , F 8 : C and fibrinogen were monitored until the 10th post traumatic day . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , the antigen and its biological activity and , the activity of plasminogen activator inhibitor ( PAI ) : were measured in twenty two healthy women during and shortly after spontaneous delivery ( 2nd stage and 3rd stage of labour , 48 and 72 hours post partum ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) regulation of type 1 plasminogen activator inhibitor ( PAI 1 ) secretion by WI 38 human fibroblasts was investigated utilizing a specific ELISA to measure PAI 1 antigen levels . ^^^ Incubation of confluent fibroblasts with t PA in serum free medium resulted in a concentration and time dependent increase in the secretion of PAI 1 , with the maximum response ( a two fold increase ) observed following a 4 hour incubation with 0 . 1 microM t PA . ^^^ Increased levels in cell associated PAI 1 were also detected following incubation of fibroblasts with t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Several blood clotting factors were studied ; von Willebrand factor ( VWF ) , fibrinogen , tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) and factor 7 . ^^^ There was no significant difference in the fibrinogen , t PA , PAI 1 or factor 7 levels between the 6 groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) activity , t PA antigen ( t PA : Ag ) and t PA inhibitor activity ( PAI ) were measured at rest and during venous occlusion ( VO ) for 10 and 20 min in 21 healthy subjects and 80 patients with venous thromboembolic disease . ^^^ Three subgroups could be identified : 10 patients ( 13 % ) with low t PA : Ag release ( PRI ) , 6 patients ( 8 % ) with a high basal level of PAI ( PR 2 ) and 9 patients ( 11 % ) who had only low t PA activity after VO . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The non responders had basal levels of PAI activity and t PA antigen higher than responders ( p less than 0 . 0001 ) and their basal levels of t PA activity were lower ( p less than 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Aspirin ( 650 mg / d 10 2 ) caused no change in resting levels of t PA antigen ( t PA : Ag ) or activity , plasminogen activator inhibitor 1 antigen ( PAI 1 : Ag ) , or activity or t PA PAI 1 complexes . ^^^ Aspirin had no effect on the increment in PAI 1 : Ag induced by venous occlusion , but similar to the effect on t PA : Ag , aspirin induced a 51 % inhibition of the increment in t PA PAI 1 complex formation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tubes containing the antiplatelet mixture CTAD could be used for determination of PAI activity , PAI antigen , vWF : Ag , FVII : Ag , t PA activity and APTT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous studies with these cells demonstrated their capacity for specific catabolism of t PA in a system modulated by plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ However , PAI 1 is not detected at the surface of HepG 2 cells in suspension , suggesting that 125I t PA . ^^^ PAI 1 , but not 125I t PA , was demonstrated for HepG 2 cells in suspension . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , squamous cell carcinoma derived cell lines secreted plasminogen activator only after treatment with the phorbol ester 12 O tetradecanoylphorbol 13 acetate ; enhanced levels of u PA , t PA , and plasminogen activator inhibitor 1 mRNAs could then be demonstrated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore , enhancement of the extrinsic fibrinolytic system is easily explained by the action of activated PC , which counteracts plasminogen activator inhibitor ( PAI 1 ) , to cause enhancement of t PA activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
They synthesize and secrete tissue type plasminogen activators ( t PA ) , urinary type plasminogen activators ( u PA ) , as well as plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ Synthesis of t PA , u PA , and PAI 1 are regulated by a variety of external agonists . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic system was monitored by analysing : plasminogen , alpha 2 antiplasmin , fibrin ( ogen ) degradation products ( FDP ) , euglobulin clot lysis time ( ECLT ) , tissue plasminogen activator ( t PA ) activity , t PA antigen and plasminogen activator inhibitor ( PAI ) activity . ^^^ The fibrinolytic system exhibited a diurnal variation with decreasing PAI activity and increasing t PA activity during the day . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Nor was spontaneous release of tissue plasminogen activator ( t PA ) or of its inhibitor ( PAI ) modified in the presence of patient ' s IgG . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this report we describe the physiologic role of PAI 1 during the interaction of the tissue plasminogen activator ( t PA ) secreting Bowes human melanoma cell line with endothelial extracellular matrices . ^^^ In the absence of plasminogen , a 104 kDa complex between Bowes t PA and PAI 1 appears in the supernatant . ^^^ PAI complex is degraded into a 68 and a 47 kDa complex by small amounts of plasmin generated from secreted Bowes t PA and plasminogen . ^^^ Analysis of these complexes revealed that t PA is rapidly cleaved by plasmin within the complex whereas complexed PAI 1 is not further degraded . ^^^ Matrix associated PAI 1 may play an important role in the protection of extracellular matrices from remodeling and degradation by cellular t PA and plasminogen . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The primary target for endothelial plasminogen activator inhibitor ( PAI ) is located within the L chain of t PA . ^^^ Deleting specific segments of t PA H chain cDNA and subsequent transient expression in mouse Ltk cells of t PA deletion mutant proteins did not affect the formation of a stable complex between mutant t PA and PAI . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of a fast acting plasminogen activator inhibitor ( PAI ) , which neutralizes both tissue plasminogen activator ( t PA ) and urokinase , are markedly increased in endotoxin treated rabbits . ^^^ Ancrod stimulated t PA activity by 90 % in normal rabbits and caused hypofibrinogenemia but did not increase PAI levels or induce fibrin deposition in target organs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of PA 1 in plasma influence the amounts of t PA precipitated in euglobulins . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this combined assay system t PA functional activity was found to depend not only on the amount of t PA antigen but also on the amount of plasminogen activator inhibitor ( PAI ) , whereas in the t PA antigen assay PAI did not affect the results . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Preoperatively , and repeated on the 3 . postoperative day , assays of euglobulin clot lysis time ( ELT ) after venous stasis , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) were performed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators are decreased in the culture media of TGF beta treated cells concomitantly with the increase in PAI 1 accumulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To test this hypothesis 12 patients undergoing infrarenal aortic reconstruction due to arteriosclerotic disease were evaluated for influence of the respiratory pattern on release of tissue plasminogen activator ( t PA ) and its inhibitor ( PAI ) in systemic and pulmonary arterial blood . ^^^ The blood samples were analysed for euglobulin clot lysis time ( ECLT ) , t PA activity , t PA antigen and PAI . ^^^ Before surgery t PA antigen was 14 . 5 + / 1 . 3 ng / ml and PAI 15 . 1 + / 2 . 8 U / ml ( mean + / SEM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This same pattern for the five antibodies was observed in an assay measuring the binding of 125I t PA to its fast acting inhibitor , PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The results from tests of euglobulin lysis time , t PA activity , t PA antigen levels , and t PA fast acting inhibitor ( plasminogen activator inhibitor [ PAI ] ) level were compared with those obtained after venous occlusion . ^^^ Intravenous desmopressin acetate induced the release of large amounts of t PA in most cases and caused a significant fall in PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After isolation , the cultures were grown to confluency and then studied under identical conditions for release of tissue plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) into serum free medium . ^^^ This PA activity on the cell surface was not stimulated by addition of CNBr fibrinogen fragments but could be partially inhibited by activated bovine PAI and antibodies against human t PA and urokinase PA , respectively . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , a high level of PAI 1 at rest was associated with a high prestasis antigen level of t PA and a low fibrinolytic response after 10 min of venous stasis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our knowledge about the components of the fibrinolytic system has greatly increased during the last few years thanks to the cloning of the cDNA of the two plasminogen activators t PA and u PA , of the two plasminogen activator inhibitors PAI 1 and 2 , and of other profibrinolytic and of inhibitory factors of this system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The method proved accurate since it correctly determined the PAI activity ( range 0 to 110U / mL ) in citrated plasma samples with levels established by time course analysis of t PA inhibition and by titration with t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Highly purified plasminogen activator inhibitors of type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , low Mr form , were compared with respect to their kinetics of inhibition of tissue type ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^ PAI 1 rapidly reacted with single chain t PA as well as with two chain forms of t PA and u PA . ^^^ The k values for inhibition of single chain t PA , two chain t PA and two chain u PA were respectively , 1200 , 150 and 8 . 5 times higher with PAI 1 than with PAI 2 . ^^^ The k values for inhibition of single chain t PA and endogenous t PA in plasma by PAI 1 or PAI 2 were identical indicating that t PA in blood consists mainly in its single chain form . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We investigated relationship between cigarette smoking and fibrinolytic system , namely the plasma level of t PA antigen , plasminogen activator inhibitor ( PAI ) , and PA activity . ^^^ The plasma levels of t PA antigen , PAI activity , PA activity and catecholamine were measured together with measurement of blood pressure and heart rate before , soon after or 30 min after cigarette smoking . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin autography of plasma supplemented with tissue plasminogen activator ( t PA ) revealed that in icteric samples most of the added activator migrated with an apparent Mr of 100 kDa , corresponding to t PA PAI complex , whereas in control samples virtually all t PA migrated as free enzyme . ^^^ In controls , venous occlusion induced an increase in both fibrinolytic activity and t PA antigen and a reduction in PAI activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using specific assays for tissue plasminogen activator ( t PA ) activity , t PA inhibitor ( PAI ) and t PA antigen , we measured these activities in 16 women who developed DVT during their pregnancies . ^^^ The site at which blood was sampled for analysis appeared to be an important criterion in the ex vivo assessment of functional t PA reserve and PAI levels , though relatively less so for the latter measurement . ^^^ The unaffected lower limbs , relative to the unaffected upper limbs , showed an increase in PAI and a demonstrable decrease in t PA release , both representing increased risk factors for rethrombosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The influence of age , gender , and aspirin ingestion on plasma levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activities was studied in a reference population of 35 men and 35 women between the ages of 20 and 65 years . ^^^ T PA showed an inverse relationship to PAI in both sexes . ^^^ There was a negative correlation of t PA levels with age , whereas PAI levels were positively correlated . ^^^ The ingestion of a single dose of aspirin ( 650 mg ) did not alter PAI or t PA activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The PAI of one of the two melanoma cell ( MJZJ ) conditioned media found to contain PAI activity was purified to apparent homogeneity employing concanavalin A Sepharose chromatography , gel filtration on Sephadex G 150 , chromatography on Affi Gel blue , and affinity chromatography on a Sepharose 4B immobilized monoclonal anti t PA IgG column . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Most of the hitherto published assays for fast acting PAI are reported to have the disadvantage of non parallel titration curves of tissue plasminogen activator ( t PA ) in buffer and plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in plasma tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) by the interferon treatment for chronic hepatitis C ] . ^^^ To determine the effects of interferon ( IFN ) treatment for chronic hepatitis C on vascular endothelium , we measured the concentrations of tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) in the plasma from patients before and after IFN treatment for 14 consecutive days . ^^^ The plasma t PA and PAI 1 levels were measured before and after treatment . ^^^ The plasma t PA level was significantly increased after IFN treatment ( p < 0 . 01 ) but no significant difference in plasma PAI 1 level was observed before and after treatment . ^^^ The ratio of t PA / PAI 1 was significantly increased after IFN treatment ( p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 , PAI 2 , and all of the chimeras retained inhibitory activity for t PA , but only the chimera containing PAI 1 residues 1 167 formed a complex with VN . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Two clusters of haemostatic factors could be distinguished in which each variable was correlated ( P < 0 . 001 ) to every other variable : ( a ) Eight fibrinolysis assays including t PA , PAI 1 and euglobulin clot lysis time ( ECLT ) , for which PAI 1 appeared to be the dominating factor ; ( b ) antithrombin 3 , protein C , alpha 2 antiplasmin and plasminogen , the interdependence of which has no obvious explanation . ( 2 ) . ^^^ C reactive protein , an acute phase reactant , was positively correlated ( P < 0 . 001 ) with fibrinogen , factor VIIIc , von Willebrand factor , the fibrinolysis assays t PA , PAI 1 , ECLT and plasminogen . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Continuous infusion of prostacyclin decreases plasma levels of t PA and PAI 1 in primary pulmonary hypertension . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS Plasma Lp ( a ) was measured by enzyme linked immunosorbent assay , lipids and lipoproteins by enzymatic methods , and apolipoproteins A 1 and B , fibrinogen ( turbidimetric method ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and D dimer levels by enzyme immunoassay . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To define structural rearrangements occurring during interaction between PAI 1 and its target proteinases we have raised monoclonal antibodies against the PAI 1 / t PA complex . ^^^ Thirteen out of 401 monoclonal antibodies reacted preferentially with the PAI 1 / t PA complex as compared to free PAI 1 or free t PA . ^^^ Detailed characterization revealed the presence of two non overlapping neoantigenic epitopes in the PAI 1 / t PA complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PmC were relevant in monitoring the fibrinolytic state in blood . 2 ) Both tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) levels were sustained continuously in the elevated levels in the blood during both UK administration and pulse therapy . ^^^ This movement of t PA and PAI 1 was independent of that of the other fibrinolytic factors , such as alpha 2 PI , alpha 2 PI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of tissue plasminogen activator antigen ( t PA : ag ) , tissue plasminogen activator inhibitor 1 antigen ( PAI 1 : ag ) and tissue plasminogen activator inhibitor activity ( PAI activity ) , and alpha 2 antiplasmin ( AAP ) were measured in plasma from 19 patients with severe trauma on admission and on days 1 , 2 , 3 and 7 after the incident . ^^^ Levels of t PA : ag , PAI 1 : ag and PAI activity were increased , and levels of AAP were low immediately after trauma . ^^^ Levels of t PA : ag normalized during the first week , whereas PAI 1 : ag levels decreased gradually from day 1 to day 3 . ^^^ Levels of APP increased significantly during the first week . t PA : ag , PAI 1 : ag or PAI activity levels were not correlated with the ISS on any day , but levels of AAP showed a weak correlation with the ISS on day 7 . ^^^ Post traumatic levels of t PA : ag and PAI 1 : ag were higher in patients who had 6 or more units of blood transfusions for resuscitation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When PAI 1 was converted into its latent form by incubation for 48 hours at 37 degrees C or incubated with an excess of recombinant t PA to convert free PAI 1 into t PA PAI 1 complexes , the stimulatory effect of both the latent and the complexed form of PAI 1 was significantly greater than that of the active form . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The search for antibodies to t PA was performed by means of several systems , allowing for the identification of epitopes presented in different conformational physical states of t PA : free or associated to its inhibitor ( PAI 1 ) in plasma , specifically bound to fibrin surface , or passively adsorbed to solid supports . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of dextran on plasma tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) during surgery . ^^^ We decided to explore the effect of dextran on plasma tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) during surgical trauma . ^^^ The activities of t PA and PAI 1 during surgery were determined , as was the concentration of t PA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients with elevated plasminogen activator inhibitor 1 ( PAI 1 ) levels or decreased release of tissue plasminogen activator ( t PA ) antigen were given information and advice about improved diet , increased physical activity , weight loss and / or cessation of smoking . ^^^ Totally 144 patients ( 119 with elevated PAI 1 and 25 with deficient t PA release ) were followed with repeated analyses of the fibrinolytic parameters for a median of 8 months after the initial advice , and 65 % of the patients managed to execute at least one change in their lifestyle . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
From four of the mixed primary tumors with distinct melanotic and amelanotic zones , the respective components were propagated separately in transgenic hosts as s . c . transplants to obtain data for clearly identifiable melanotic versus amelanotic parts . u PA and PAI 1 mRNAs were expressed in all . t PA expression varied greatly and was notably high in several amelanotic tumors or tumor components , possibly as a result of large blood vessels , as such vessels were seen to be t PA positive in normal tissue . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following parameters were evaluated at the time 0 and after 2 , 4 , 6 , 8 , 10 and 12 hours from each administration : tissue plasminogen activator ( t PA ) and its inhibitor PAI 1 , euglobulin lysis time , plasminogen and alfa 2 antiplasmin as indexes of the fibrinolytic system ; aPTT , TT , fibrinogen as indexes of the hemostatic coagulative system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following parameters were evaluated : tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , euglobulin lysis time ( ELT ) , plasminogen , alpha 2 antiplasmin , prothrombin time ( PT ) , activated partial thromboplastin time ( aPTT ) , thrombin clotting time ( TCT ) , and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total cholesterol , HDL cholesterol ( HDL C ) and its subfractions ( HDL 2 C and HDL 3 C ) , blood glucose , Apolipoproteins A 1 and B , fibrinogen , plasminogen , factor 7 , t PA : Ag and PAI activity pre and post venous occlusion ( VO ) were determined . ^^^ After 12 weeks of treatment in the gemfibrozil group the release of t PA : Ag in response to venous occlusion was significantly higher and plasma PAI activity was significantly lower than in placebo group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serial changes in plasma plasminogen activator inhibitor 1 ( PAI 1 ) , plasma tissue plasminogen activator ( t PA ) antigen and serum lipoprotein ( a ) levels were compared between the two groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of the fast acting t PA inhibitor , normally referred to as PAI 1 , were considerably higher in hypertensives ( 9 . 22 vs . 4 . 41 IU mL 1 ; P < 0 . 02 ) , and this difference persisted in the upright posture , indicating a decrease in fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For each sample the determination of seven prothrombotic markers [ ( fibrinogen ( F ) , factor 7 ( F 7 ) , antithrombin 3 ( AT 3 ) , protein C ( PC ) , protein S ( PS ) ( coagulometric method IL ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) ( ELISA method Boehringer ) ] and of three prethrombotic markers [ ( fibrinopeptide A ( FPA ) , beta thromboglobulin ( BTG ) and D dimer ( D D ) ( ELISA method , Boehringer ) ] was performed . ^^^ The results obtained in the group of the cerebrovasculopathic patients compared to the controls showed a significant increase of F ( p < 0 . 001 ) , F 7 ( p < 0 . 005 ) , BTG ( p < 0 . 05 ) and D D ( p < 0 . 01 ) , whereas significant differences regarding AT 3 , PC , PS , t PA , PAI and FPA were not observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 PA inhibitor ( PAI 1 ) mRNA was expressed at the base of the necrotic atheroma of all specimens and also within some of the inflammatory infiltrates where it frequently colocalized in regions containing u PA and t PA mRNA expressing cells . ^^^ However , in these areas , the cellular distribution of the transcripts for t PA and u PA extended far beyond the areas of PAI 1 expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the septic baboons , a significant reduction of the formation of thrombin ( peak TAT levels decreased from 582 + / 78 ng / mL to 219 + / 106 ng / mL ; P < . 005 ) , the release of t PA ( peak levels decreased from 37 + / 11 ng / mL to 17 + / 2 ng / mL ; P < . 001 ) , and its inhibitor , PAI ( peak levels decreased from 2 , 639 + / 974 ng / mL to 1 , 110 + / 153 ng / mL ; P < . 001 ) , was observed in the group receiving IL 1ra compared to that receiving placebo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma was assayed for clotting and fibrinolytic activity by measuring thrombin / antithrombin complexes ( TAT ) , prothrombin fragment 1 + 1 ( F 1 + 2 ) , and D dimers ( D di ) ; markers of the endothelial based fibrinolytic components were assayed by measuring tissue plasminogen activator antigen ( t PA Ag ) and its inhibitor ( PAI 1 ) ; finally , the activity of antithrombin 3 , protein C , and protein S were measured . ^^^ Non steroid users were more likely to have elevated levels of t PA Ag and PAI 1 than our steroid using weight lifters ( both P < . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Twelve healthy male volunteers , mean age twenty five , range twenty one to thirty years , and 12 healthy middle aged male volunteers mean age fifty eight , range forty four to seventy two years , were tested regarding platelet aggregation induced by adenosine diphosphate and fibrinolytic activity , estimated as euglobulin clot lysis time ( ECLT ) , tissue plasminogen activator ( t PA ) , and the fast acting inhibitor against t PA normally referred to as ( PAI 1 ) . ^^^ Fibrinolytic activity decreased significantly in the middle aged group as shown by a prolongation of the ECLT ( P < 0 . 01 ) and PAI 1 , although not significantly , increased by approximately 100 % , whereas t PA significantly increased in the middle aged group ( P < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In septic patients capable of normal white cell responses , high plasma levels of PAI 1 , t PA antigen and t PA PAI 1 complex were observed . ^^^ The ratios of t PA and PAI 1 were such that free PA activity was almost never observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the whole population of MI patients and in patients with a family history of thrombosis t PA antigen was positively correlated with PAI 1 antigen and vWF . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When t PA is bound to endothelial cells ( EC ) , its catalytic efficiency is increased and is prevented from being inhibited by its inhibitor PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Elisa tests for t PA , PAI 1 , u PA , FgDP , TDP , and D Di levels were used for measurements of fibrinolytic activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate further the activation status of the two systems , levels of thrombin antithrombin complexes ( TAT ) , prothrombin fragment 1 + 2 , protein C , total and free protein S , C4bBP , antithrombin 3 , t PA , PAI 1 and D dimer were measured . ^^^ PC was low in 4 cases , fPS in 6 and tPS in 2 , whereas C4bBP and ATIII values were within normal range . t PA and D dimer levels were high in 11 / 14 patients , reaching 20 ng / ml ( 2 . 7 106 ) and 1660 ng / ml ( 877 3780 ) respectively , while PAI 1 was considerably increased in the 2 severe cases and normal in the remainder . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this population based case control study , we examined the relationship between the fibrinolytic variables tissue plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) activity , cardiovascular risk factors and peripheral arterial disease . ^^^ Mean levels of t PA antigen and PAI activity were significantly elevated in 121 cases compared to 126 controls . ^^^ The increased risks of peripheral arterial disease with increasing PAI activity and t PA antigen levels were partly mediated by interactions with serum triglycerides , high density lipoprotein ( HDL ) cholesterol and cigarette smoking . ^^^ For example , adjustment for triglycerides significantly reduced the odds of disease for PAI activity from 1 . 41 ( 95 % confidence intervals 1 . 08 , 1 . 86 ) to 1 . 24 ( 0 . 93 , 1 . 65 ) and from 1 . 47 ( 1 . 09 , 1 . 98 ) to 1 . 34 ( 0 . 99 , 1 . 82 ) for t PA antigen . ^^^ We conclude that impaired fibrinolytic potential ( raised PAI activity and t PA antigen ) is associated with peripheral atherosclerosis and that this relationship is partly influenced by lipids and cigarette smoking . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Conditioned media were assayed for urokinase type and tissue type PA ( u PA and t PA , respectively ) , PA inhibitor 1 ( PAI 1 ) , and PA activity . ^^^ The PA activity of conditioned medium decreased after stimulation with progesterone , and this was secondary to a decrease in u PA , but not t PA , and an increase in PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 10 patients t PA and PAI 1 activities were also measured and plethysmography was carried out . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In these studies we examined the effects of HSV infection on HUVEC plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrin degradation is plasmin dependent and is regulated by the balance between plasminogen activators ( PA ) , tissue PA ( t PA ) , urokinase PA ( u PA ) , and their inhibitors ( PAI ) . ^^^ Fibrin induction of t PA was selective because the levels of u PA ( 45 kD ) , PAI 1 ( 50 kD ) , or protein synthesis in general were unaffected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Chromogenic peptide substrate analytical method was used to detect plasma tissue plasminogen activator ( t PA ) , tissue plasminogen activator inhibitor ( PAI ) activity and ratio of P / t . ^^^ The result revealed that the activity of plasma t PA in presenile geriatric period subjects was significantly higher than that of young people ( P < 0 . 01 ) , but the activity of PAI had no difference in various age groups , and the ratio of P / t was markedly decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The objective of the present study was to assess the effect of EPO in dialyzed patients on two key enzymes of fibrinolysis , i . e . the tissue activator of plasminogen ( t PA ) and the inhibitor of the plasminogen activator ( PAI 1 ) . ^^^ In eight patients dialyzed for prolonged periods examined under otherwise equal conditions before EPO treatment ( haematocrit 22 . 9 % median value ) and after 9 . 5 weeks of EPO treatment ( Recormon , s . c . ) when a haematocrit of 30 % was achieved , activities ( chromogenic substrates ) and antigens ( ELISA of t PA and PAI ) were assessed . ^^^ Similarly as in the case of activities in antigens t PA and PAI no difference was found before and during EPO . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of von Willebrand Factor , PAI 1 antigen , t PA antigen ; variables produced by the endothelial cell , increased during the third trimester of pregnancy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
D dimer , plasminogen activator inhibitor ( PAI 1 ) activity at rest and after exercise , and tissue plasminogen activator ( t PA ) activity after exercise were measured in venous blood in 88 patients with atherosclerotic lesions of various degrees . ^^^ There were correlations ( P < 0 . 05 ) between : age and D dimer ( r = 0 . 29 ) ; D dimer and t PA ( r = 0 . 34 ) ; D dimer and PAI 1 , r = 0 . 29 . ^^^ In patients with the highest level of D dimer , the lowest level of PAI 1 activity and the highest level of t PA activity after exercise were observed . ^^^ The low PAI 1 activity is probably the result of an increased release of t PA in these patients . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Expression of PAI 1 , t PA and u PA in cultured human umbilical vein endothelial cells derived from racial groups . ^^^ To determine whether inherent fibrinolytic differences may exist in racial groups ( black americans , BA vs . white americans , WA ) , 55 different individual racially derived human umbilical vein endothelial cell ( HUVEC ) cultures ( 35 BA and 20 WA ) were analyzed in terms of their fibrinolytic protein ( t PA , u PA and PAI 1 ) antigen and mRNA levels . ^^^ These combined data suggest that cultured HUVECs from BA express significantly higher levels of t PA , lower levels of PAI 1 and approximately 1 . 72 fold lower molar ratio of PAI 1 / t PA antigen ( 183 . 99 + / 168 . 81 vs . 315 . 92 + / 164 . 99 ) ( p < 0 . 05 ) than cultured HUVECs from WA , presumably reflecting an apparent inherent increased fibrinolytic potential in cultured HUVEC derived from BA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Methods for measuring antigen and activity of plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) and their complex have been improved in the past few years , but few comparative data are available and they should be standardized . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following tests were carried out before and after the VO test : euglobulin lysis time and t PA and PAI 1 activities and antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrinogen , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) activity were measured at 3 month intervals . ^^^ Placebo treatment did not significantly alter the plasma levels of fibrinogen or t PA and PAI activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PDGF , t PA , PAI 1 and ET 1 plasma levels were measured before and after a 2 month treatment period with trapidil 200 mg tablets bid or placebo in 30 patients affected by POA in Fontaine stage 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The liver plays many roles in this regulation , including the synthesis of plasminogen , alpha 2 antiplasmin ( alpha 2 AP ) and plasminogen activator inhibitor 1 ( PAI 1 ) , and the clearance of tissue plasminogen activator ( t PA ) . ^^^ Inherited deficiencies of alpha 2AP , PAI 1 and inherited excess t PA associated with clinically significant bleeding have been reported . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased tissue plasminogen activator ( t PA : Ag ) antigen 4 hours after AraC application ( p < 0 . 05 ) as well as increased levels of plasminogen activator inhibitor activity ( PAI ) ( p < 0 . 05 ) and fibrinopeptide A ( FPA ) antigen ( p < 0 . 05 ) were observed on day 2 . ^^^ During that period of infection the increased levels of FPA on day 21 ( p < 0 . 05 ) , PAI on days 15 and 21 ( p < 0 . 05 ) and fibrinogen on day 21 ( p < 0 . 05 ) as well as decreased values of antithrombin 3 ( p < 0 . 05 ) on day 21 and protein C on day 15 ( p < 0 . 05 ) were measured . t PA : Ag , plasminogen , alpha 2 antiplasmin and fibrin ( ogen ) degradation products were within normal throughout infectious complications . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The radioactivity and immunological level of t PA and PAI 1 in the solution eluted to each fraction by gel permeation chromatography were measured by means of a well scintillation counter and enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , on collagen coated dishes , cellular levels of t PA , PAI 1 , 72 kD type 4 collagenase , TIMP 1 , and TIMP 2 mRNA were not greatly changed during incubation for 24 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of the plasminogen activators u PA and t PA as well as the inhibitor PAI 1 were studied immunohistochemically in 35 osteosarcoma specimens compared to 15 Ewing ' s sarcomas and various other bone lesions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of t PA , is an unusual member of the serpin family of serine protease inhibitors , in that it spontaneously converts to a latent form . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of t PA , is an unusual member of the serpin family of serine protease inhibitors , in that it spontaneously converts to a latent form . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Net release or uptake were assessed by arterio venous ( AV ) concentration gradients across the forearm of t PA antigen and t PA activity , and plasminogen activator inhibitor antigen type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the ESRD patients , the insulin levels showed a positive correlation with C peptide ( r = 0 . 612 , p = 0 . 0001 ) , fasting blood sugar ( r = 0 . 334 , p = 0 . 044 ) , and PAI 1 antigen ( r = 0 . 474 , p = 0 . 0001 ) and a reverse correlation with euglobulin fibrinolytic activity ( r = 0 . 5 , p = 0 . 005 ) , but no correlation with t PA antigen . ^^^ The euglobulin fibrinolytic activity showed a reverse correlation with PAI 1 antigen ( r = 0 . 289 , p = 0 . 0144 ) , but no correlation with t PA antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Treatment of endothelial cells with either IL 1 beta or TNF alpha under static conditions increased PAI 1 , vWF and prostacyclin release , while t PA secretion was unchanged . ^^^ When cells were exposed to steady shear stress of 0 , 6 , 12 , 18 and 24 dyne / cm2 , the release of t PA , t PA PAI complex and prostacyclin elevated with the increase of shear stress intensity , while a gradual decrease of total PAI 1 secretion was observed and vWF secretion was unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Median levels of soluble fibrin , fibrinogen degradation products , D dimer and PAI 1 were increased , while the level of t PA activity remained constant . ^^^ The increase in PAI 1 levels intra CPB appeared to result in mean t PA activity remaining unchanged 1 h post CPB . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Heat stroke patients showed ( 1 ) significant prolongation of the prothrombin ( PT ) , activated partial thromboplastin ( aPTT ) and thrombin times ( TT ) but normal reptilase time ( RT ) ; ( 2 ) significant reduction in plasma levels of antithrombin 3 ( AT 3 ) , factor 5 , proteins C and S , plasminogen activator inhibitor ( PAI ) and platelet count ; ( 3 ) increase in plasma factor 8 , tissue plasminogen activator ( t PA ) and serum FDP ; ( 4 ) no significant changes in plasma fibrinogen , plasminogen , alpha 2 antiplasmin and factors 7 and 10 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , intra individual comparisons were made of the concentrations of t PA , urokinase type plasminogen activator ( u PA ) , PAI 1 , and PAI 2 in GCF from the same sites before and after periodontal treatment in eight healthy male volunteers aged 35 46 yr . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Staining of t PA , u PA , and PAI was observed in all the metastases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Variations in the major parameters of fibrinolysis ( euglobulin lysis time , t PA antigen , PAI 1 antigen , PAI 1 activity ) were studied in an open randomized cross over study in 21 healthy volunteers , in which three types of treatment were tested for periods of 24 h each ( without elastic compression , elastic compression of an upper limb , elastic compression of the lower limbs ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of beta thromboglobulin ( BTG ) , platelet factor 4 ( PF 4 ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) and plasminogen were determined in 36 essential hypertensive and 20 age and sex matched control subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relationship between plasma Lipoprotein ( a ) ( Lp ( a ) ) levels and plasminogen activator inhibitor ( PAI ) activity and tissue plasminogen activator ( t PA ) antigen levels were studied in 15 patients with unstable angina . ^^^ On the other hand , the plasma levels of PAI activity ( IU / ml ) and t PA antigen ( ng / ml ) in patients with unstable angina were significantly higher on admission than after treatment ( PAI activity : 11 . 4 + / 1 . 4 vs 7 . 7 + / 1 . 5 , t PA antigen : 8 . 7 + / 0 . 9 vs 7 . 0 + / 0 . 9 , p < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This review presents the structural determinants on the t PA molecule that mediate clearance via these two pathways , gives an overview of the structure of the LRP / alpha 2 MR and discusses the role of plasminogen activator inhibitor type 1 ( PAI 1 ) in the hepatic clearance of t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We compared the response of the t PA plasminogen activator inhibitor type 1 ( PAI 1 ) fibrinolytic system in patients with preinfusion levels of active t PA below or at the detection limit of the assay with that in patients with higher preinfusion levels of active t PA . ^^^ In contrast , the preinfusion levels of PAI activity were significantly higher in patients with undetectable plasma levels of active t PA compared with patients with higher levels of active t PA ( 22 . 3 versus 12 . 8 IU / ml ; P < 0 . 01 ) . ^^^ CONCLUSION : Our study strongly suggests that a high plasma level of PAI 1 , the main inhibitor of t PA , is responsible for impaired t PA mediated fibrinolysis in patients with ischaemic heart disease , and that treatment with diuretics may be associated with an unfavourable effect on the fibrinolytic system . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentrations of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) were measured in blood obtained from pregnant women to elucidate the fluctuations in the fibrinolytic system which occur during the course of pregnancy . ^^^ PAI activity was determined with a competitive inhibition assay of t PA activity . ^^^ The t PA antigen and PAI activity levels rose slowly during the course of pregnancy , and fell promptly after delivery . t PA activity and t PA antigen in levels in umbilical cord blood were higher after vaginal delivery than after cesarean section . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were mutual relations among fibrinolytic parameters such as tissue plasminogen activator ( t PA ) antigens , total plasminogen activator inhibitor 1 ( PAI 1 ) , t PA PAI 1 complex , free PAI 1 , and ECLT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Hormonal intake was followed by decreased antigen concentrations of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( type 1 [ PAI 1 ] ) , whereas the activities of t PA and PAI 1 were unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared with the plasma values of healthy control children , levels of tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor type 1 ( PAI 1 ) activity , and vWF as well as fibrin ( ogen ) degradation products were significantly elevated in the plasma of HUS patients on admission . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The conversion of plasminogen into serine protease plasmin with fibrinolytic activity depends on the actual balance between plasminogen activators ( urokinase type ; u PA and tissue type ; t PA ) and their inhibitors ( type 1 and 2 plasminogen activator inhibitors ; PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared to the postabsorptive state , plasminogen activator inhibitor ( PAI 1 ) activity and antigen , tissue plasminogen activator ( t PA ) antigen and FFA decreased ( p < 0 . 001 ) after 120 min of euglycemic hyperinsulinemia . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endothelial cell markers ( vWF , t PA and PAI 1 ) in the elderly . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the same subjects we also measured plasma antigen levels of tissue type PA ( t PA ) , urokinase type PA ( u PA ) , PAI 1 , PAI 2 , and D dimer . ^^^ Plasma PAI 1 and t PA antigens were significantly augmented in patients ( p < 0 . 005 ) , the mean increase of PAI 1 being about 5 times higher than that of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thus , the unstimulated serum D dimer test , which reflects both t PA and PAI 1 , could be recommended as a global screening method for evaluation of the fibrinolytic potential . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In most of cases the impaired fibrinolytic potential after venous occlusion test was due to increased PAI 1 levels ; only in a few instances a defective fibrinolytic response was due to reduced t PA release , a combined defect or an intrinsic fibrinolytic deficiency . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The antigen levels of von Willebrand factor ( vWF ) , tissue plasminogen activator ( t PA ) , and its inhibitor ( PAI ) were also determined as markers of release from the endothelium , while the fragment 1 + 2 of prothrombin ( F 1 + 2 ) and thrombin antithrombin 3 ( TAT ) complexes were assessed as indexes of systemic thrombin generation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All the haemostatic ( figrinogen , Factor 7 , plasminogen activator inhibitor ( PAI ) activity and tissue plasminogen activator ( t PA ) antigen ( Ag ) pre venous occlusion ( VO ) ) and haemorheological parameters ( haematocrit , whole blood filterability , blood and plasma viscosity ) were significantly higher in the abdominal obesity group as compared to the control group . ^^^ Moreover positive correlations between WHR and plasma insulin ( r = 0 . 68 , p < 0 . 05 ) , between WHR and fibrinogen ( r = 0 . 63 , p < 0 . 05 ) and between WHR and PAI pre VO ( r = 0 . 71 , p < 0 . 05 ) and a negative correlation between WHR and t PA ( Ag ) post VO ( r = 0 . 55 , p < 0 . 05 ) were found . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We quantitated urokinase and tissue plasminogen activator ( u PA , t PA ) , plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) , and fibrinolytic activity in peripheral blood ( PB ) , tumour blood ( TB ) , peritoneal / ascitic fluid ( PAF ) and cystic fluid ( CF ) from 104 patients with benign and 36 patients with malignant ovarian tumours , and in peripheral blood from 62 healthy controls . ^^^ High levels of t PA , PAI 1 and PAI 2 were found in PAF of malignant tumours , and resorption from this compartment may explain elevated PB levels in patients with ascites . ^^^ However , an index based on PAF levels of t PA and PAI 1 discriminated between malignant and benign ovarian cysts in the absence of ascites . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cross linked fibrin degradation products ( XL FDP ) , tissue plasminogen activator ( t PA ) antigen , fibrinopeptide A ( FPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) activity were measured in peripheral blood . ^^^ XL FDP and t PA increased , and FPA and PAI 1 decreased significantly after angioplasty . ^^^ The alterations in PAI 1 showed no correlation with those of t PA , whereas heparin had a sparing effect on PAI 1 consumption . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the behaviour of the coagulation variables antithrombin 3 ( ATIII ) , protein C , thrombin / antithrombin 3 ( TATIII ) ; fibrinolytic activity , tissue plasminogen activator antigen ( t PA ) , plasminogen activator inhibitors ( PAI ) 1 and 2 , and endothelial involvement by fibronectin assay in normal and pre eclamptic pregnancies . ^^^ MAIN OUTCOME MEASURES : Plasma levels of PAI 1 , PAI 2 and t PA antigen were determined using an ELISA technique as were TATIII complex levels of fibronectin . ^^^ PAI 2 would seem to be a marker of placental function in pre eclampsia while increased t PA and TATIII complex levels reflect the severity of the condition . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There was no specific effect of either t PA or urokinase overexpression on endogenous t PA , urokinase , or PA inhibitor 1 ( PAI 1 ) expression . ^^^ The majority of secreted urokinase accumulated in conditioned medium as a free proenzyme , whereas both wild type and serpin resistant t PA accumulated almost exclusively in complexes with PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It is thus suggested that the balance between t PA and PAI 1 is important for the regulation of fibrinolysis . ^^^ The release of t PA and PAI 1 from vascular endothelial cells is regulated by physiological factors including thrombin ( 3 , 7 ) , histamine ( 8 ) , vasoconstrictor peptide endothelins ( 9 , 10 ) and cytokines ( 11 ) . ^^^ In addition , the regulation of the t PA release and that of the PAI 1 release are not necessarily coupled . ^^^ It has been shown that activated protein kinase C and cyclic AMP are involved in the stimulation and suppression , respectively , of the endothelial t PA and PAI 1 production ( 12 , 13 ) . ^^^ However , the role of intracellular calcium in the regulation of endothelial t PA and PAI 1 release has remained to be elucidated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was performed to investigate the acute effects of physiologically induced hyperinsulinemia on plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and triglycerides ( TG ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aims of this study were to determine whether elevated plasminogen activator inhibitor type 1 ( PAI 1 ) activity after myocardial infarction reflects baseline PAI 1 or represents an acute phase response secondary to the infarction , and to determine how tissue plasminogen activator ( t PA ) activity and total t PA antigen levels in healthy control subjects differ from those in patients after myocardial infarction . ^^^ Overall , survivors of myocardial infarction have reduced t PA activity and increased PAI 1 activity that is not due to a prolonged acute phase response . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , elevated temperatures markedly increased plasminogen activator inhibitor 1 while decreasing t PA secretion , though after prolonged exposure to 43 degrees C the levels of both returned to control values . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma PAI 1 antigen levels were increased dose dependently ( up to 210 + / 29 % of control values at a dose of 3 mg / kg ) whereas no significant effects on plasma t PA antigen levels were observed ( 131 + / 6 % compared with control values ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The available PAI 1 activity was tested indirectly using anti human PAI 1 antibody blocking before t PA activity assay ( n = 11 ) . ^^^ However , pretreatment of samples with PAI 1 antibody or by acidification ( pH 3 . 2 ) to inactivate inhibitors did not increase t PA activity levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The rates of plasminogen activator inhibitor ( PAI 1 ) , fibrinogen , plasminogen , and alpha 2 antiplasmin , along with the venous occlusion test to determine tissular plasminogen activator ( t PA ) , both functional and antigenic , before and after venous occlusion , were tested in all cases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The B 16 cell lines did not secrete any gelatinase , but they secreted TIMP 2 , tissue type ( t PA ) , urokinase type ( u PA ) plasminogen activators and PAI 1 like activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before and after a venous occlusion test were also measured : t PA Antigen , PAI activity and haematocrit . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Arterial and coronary sinus blood were sampled concomitantly before cardioplegia and after release of the aortic cross clamp , for measurement of t PA antigen ( Ag ) and activity , plasminogen activator inhibitor ( PAI 1 ) Ag and activity , t PA / PAI 1 complex , single chain urokinase ( sc uPA ) and urokinase ( uPA ) plasminogen activators , the fibrin split product D dimer , thrombin antithrombin complex ( TAT ) , and the prothrombin split product F 1 + 2 . ^^^ Cardiopulmonary bypass significantly increased t PA Ag and activity , t PA / PAI complex , D dimer , TAT , and F 1 + 2 , and decreased PAI 1 Ag and activity in arterial blood ; uPA and sc uPA were unchanged . ^^^ Tissue plasminogen activator activity and t PA / PAI complex increased , PAI 1 activity decreased , while all other parameters were unchanged across the coronary circulation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TNF alpha enhances urokinase plasminogen activator ( u PA ) activity and steady state mRNA levels twofold without affecting tissue plasminogen activator ( t PA ) or PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We performed three extractions : on day one , day five and following the withdrawal of the fat emulsion , with study of modifications to antithrombin 3 , alpha 2 antiplasmin , plasminogen , C protein , plasminogen tissular activator ( t PA ) , plasminogen tissular activator inhibitor ( PAI 1 ) , fibrinogen , and fibrinogen / fibrin degradation products . ^^^ In conclusion , it is suggested that antithrombin 3 deficiency , along with reduction in C protein levels and increased fibrinogen and PAI 1 concentration predispose patients with t PA release defects to thrombo embolic complication . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , lysis of the model platelet rich thrombus did proceed some 30 % more quickly when treated with a plasminogen activator inhibitor ( PAI ) resistant t PA variant . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we measured the antigen levels of urokinase ( u PA ) , tissue plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , and type 2 plasminogen activator inhibitor ( PAI 2 ) , and cancer tissue ( 19 adenocarcinomas and 19 squamous cell carcinomas ) and normal lung tissue . ^^^ RESULTS . u PA , PAI 1 , and PAI 2 antigen levels in cancer tissue were significantly higher than those in normal tissue ( P < 0 . 001 in u PA and PAI 1 ; P < 0 . 005 in PAI 2 ) , whereas t PA antigen levels in cancer tissue were significantly lower than those in normal tissue ( P < 0 . 005 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No differences in t PA and PAI concentration were found . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It could be demonstrated that this PAI 1 binding protein had a stabilizing effect on the PAI 1 activity without interfering with the kinetics of the inhibition of t PA by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS Plasminogen activator inhibitor ( PAI ) activity and concentrations of tissue plasminogen activator ( t PA ) antigen were measured in venous plasma samples taken at 2200 , 0600 , and 1400 and 24 h Holter tapes were recorded in 15 patients with variant angina , 12 patients with stable exertional angina , and 12 controls . ^^^ RESULTS There were significant circadian variations in PAI activity and t PA antigen with peak values at 0600 in all three groups . ^^^ Furthermore , both plasma PAI activity and t PA antigen concentrations were significantly higher in the variant angina group than in the stable exertional angina group and the control group at each sampling time . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
High plasma PAI activity was accompanied by an increased resistance of plasma clots to exogenous t PA induced fibrinolysis . ^^^ Since PAI depleted plasma clots however displayed a weak resistance to t PA induced fibrinolysis , it was concluded that plasma PAI may be the main , but not the only factor , responsible for the prolonged plasma clot lysis time in obese subjects . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The normal global fibrinolytic activity despite increased t PA activity may be due to a concomitant increase in PAI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The endothelium release t PA inhibitor ; PAI 1 , prostacyclin and endothelin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIMS To identify the relative contribution of plasminogen activators , particularly tissue plasminogen activator ( t PA ) and specific plasminogen activator inhibitors ( PAI 1 , PAI 2 ) , to the fibrinolytic changes associated with various types of liver disease or severe chemical and physical damage to the liver . ^^^ METHODS Platelet rich ( PRP ) and platelet poor plasma ( PFP ) from patients with alcoholic cirrhosis , primary biliary cirrhosis , hepatic malignancy , or paracetamol overdose , or who were undergoing partial hepatectomy or liver transplantation , were assayed for t PA , PAI 1 , t PA PAI 1 complex and PAI 2 antigen values using specific enzyme linked immunosorbent assays ( ELISAs ) developed in this laboratory . ^^^ RESULTS Appreciable increases in the plasma concentration of t PA , PAI 1 , and t PA PAI 1 were seen in patients with alcoholic cirrhosis , primary biliary cirrhosis , and hepatic malignancy . ^^^ Liver damage due to paracetamol overdose and partial hepatectomy both resulted in a striking increase in plasma PAI 1 concentration , although concentrations of t PA and t PA PAI 1 complex were less affected . ^^^ Concentrations of t PA , PAI 1 , and t PA PAI 1 complex returned to near normal values after successful liver transplantation in a patient with chronic active hepatitis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effects of different treatments on plasma t PA and PAI activity in AMI ] . ^^^ In the present study , we determined and compared plasma t PA and PAI activity in 58 AMI patients receiving thrombolytic therapy with UK and snake venom antithrombus enzyme ( SVATE ) or routine therapy during 3 weeks , and also in 24 healthy subjects . ^^^ Plasma t PA activity was decreased and plasma PAI activity increased significantly in patients with AMI as compared to normal controls . ^^^ UK induced an immediate and transient increase in t PA and a decrease in PAI activity ( P < 0 . 001 , respectively ) , followed by an increase in PAI activity until 21 days . ^^^ Treatment with SVATE caused an increase in t PA activity which remained above the normal level at 21 days , and a decrease in PAI activity which reached the normal level by 7 days . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
If these commoner defects are not found , the rarer defects , including HC 2 , plasminogen or t PA deficiency , dysfibrinogenemia , or elevated PAI 1 , should next be sought . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of antithrombin 3 ( AT 3 ) , protein C ( PC ) , plasminogen ( Pg ) and alpha 2 antiplasmin ( AP ) , total and free protein S ( PS ) , thrombin antithrombin 3 complex ( TAT ) , F1 . 2 fragment of prothrombin ( F1 . 2 ) , fibrinogen ( Fg ) , soluble fibrin monomers ( FM ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , total fibrinogen / fibrin degradation products ( TDP ) and D dimer ( DD ) were determined prior to the therapeutic regime , at the end of the treatment , and 24 hours later . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Its overall effect will depend primarily on the concentrations of Lp ( a ) , PAI 1 and t PA in vivo . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The relation of habitual diet and cardiorespiratory fitness to plasma fibrinogen concentration , Factor 7 activity ( F VIIc ) , Factor 10 activity ( F 10 ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) concentrations , anti thrombin 3 ( AT 3 ) and apolipoprotein ( a ) ( Apo [ a ] ) was analyzed in 111 normolipidemic men aged 51 53 years . ^^^ Plasma fibrinogen was measured by thrombin method , F VIIc by one stage coagulation method , AT 3 and F 10 colorimetrically , t PA and PAI 1 antigens by ELISA and Apo ( a ) concentration radioimmunologically . ^^^ Carbohydrate intake was negatively ( r = 0 . 31 , p < 0 . 001 ; r = 0 . 24 , p < 0 . 01 ; r = 0 . 36 , p < 0 . 001 ) and fat intake positively ( r = 0 . 24 , p < 0 . 01 ; r = 0 . 29 , p < 0 . 001 ; r = 0 . 32 , p < 0 . 001 ) related to F 10 , PAI 1 , and t PA , respectively . ^^^ Fasting insulin was the strongest determinant for PAI 1 ( r = 0 . 55 , p < 0 . 001 ) and a significant positive correlate to F VIIc ( r = 0 . 30 , p < 0 . 001 ) , F 10 ( r = 0 . 28 , p < 0 . 01 ) and t PA ( r = 0 . 47 , p < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Components of the fibrinolytic system ( i . e . , PAI 1 , t PA , fibrinogen , plasminogen , and alpha 2 antiplasmin ) were determined in 100 patients with AMI . ^^^ Thus , 54 patients ( 54 % ) had hypofibrinolysis , due to increased PAI 1 in 41 cases ( 41 % ) , impaired t PA release in 13 cases ( 13 % ) and both causes in 12 cases ( 12 % ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At the same time , fibrinolytic activity increased markedly : euglobulin clot lysis time shortened from 178 to 68 min , PAI 1 fell from 8 . 91 to 5 . 16 IU ml 1 , and t PA rose from 0 . 56 to 3 . 95 IU ml 1 , all three values were significant to P < 0 . 01 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator activity ( PAA ) , tissue type PA ( t PA ) antigen level , plasminogen activator inhibition ( PAI ) , and plasmin inhibition ( PI ) showed no sex dependent differences in the stomach of the rat . ^^^ Chronic ethanol consumption induced an increase of PAA and t PA antigen and a decrease of PAI ( urokinase type PA [ u PAI ] ) and PI in the body and pyloric region ( PI only in the pyloric region ) . ^^^ The effects of orchidectomy and chronic ethanol consumption on gastric PAA , t PA antigen , and PAI were additive . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic system is part of the defense against thrombotic and cardiovascular events , but so far no study has shown that clinical measurements of fibrinolytic key components such as tissue plasminogen activator ( t PA ) or plasminogen activator inhibitor type 1 ( PAI 1 ) have any predictive value beyond 3 years . ^^^ In 1983 through 1985 , 213 consecutive patients with angina pectoris and angiographically verified coronary artery disease were sampled , and the mass concentration of t PA and the activity of PAI 1 were measured in citrated plasma samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It is demonstrated that deviation in the t PA / PAI 1 system indicate endothelial cell dysfunction in patients with IHD . ^^^ The experimental and clinical studies reviewed provide good evidence that the endothelial cell response of t PA and PAI 1 is coupled and that there is an intimate interrelation between generation of thrombin and production / release of t PA and PAI 1 in IHD patients . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A slow clearing , fibrin specific , PAI 1 resistant variant of t PA ( T103N , KHRR 296 299 AAAA ) . ^^^ It shows substantial resistance to the fast acting inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , requiring a 10 fold greater molar excess of PAI 1 to reduce its activity by 50 % , compared to t PA . ^^^ These results show that it is possible to combine mutations in different domains of t PA to construct a variant which is simultaneously slower clearing , less reactive towards plasminogen in the absence of a fibrin clot , and resistant to inactivation by PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the accompanying paper , we reported that the properties of decreased plasma clearance rate , increased fibrin specificity , and resistance to inactivation by PAI 1 could be effectively combined in the t PA variant T103N , KHRR 296 299 AAAA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We then measured the antigen levels of total PAI 1 and t PA PAI 1 complex in the lyzed solutions of PRP clot and PPP clot to analyse the possible effect of plasminogen activator inhibitor 1 ( PAI 1 ) present in platelets . ^^^ Most of PAI 1 in a PPP clot lyzed sample existed as t PA PAI 1 complex . ^^^ In the lyzed solution of PRP clot , however , the antigen levels of both total PAI 1 and t PA PAI 1 complex were significantly higher than those in PPP clot , and larger amounts of PAI 1 existed as free PAI 1 which possesses activity . ^^^ These data suggest that at least certain amounts of PAI 1 in platelets exist as an active form and inhibits t PA activity resulting in the prolongation of the clot lysis time . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of t PA ( tissue plasminogen activator ) and t PA PAI 1 ( plasminogen activator inhibitor 1 ) complex increased with increase in age , but those of PAI 1 ( total and free ) did not change in controls . ^^^ Plasma levels of t PA , t PA PAI 1 complex , free and total PAI 1 increased with increase in body mass index in controls , but no significant changes were shown in these parameters in DM patients . ^^^ When compared with controls , plasma levels of t PA , t PA PAI 1 complex and PAI 1 were lower in DM patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Expression of plasminogen activators ( PA ) , tissue type ( t PA ) and urokinase type ( u PA ) , as well as PA inhibitors ( PAI ) , type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , were investigated immunohistochemically in 97 human pancreatic carcinomas . u PA expression predominated in pancreatic carcinomas , compared with t PA [ u PA expression in 76 specimens ( 78 . 4 % ) and t PA in eight specimens ( 8 . 2 % ) ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator antigen ( t PA Ag ) , plasminogen activator inhibitor ( PAI ) activity , and factor 8 von Willebrand factor ( vWF ) were measured before and 10 minutes after venous occlusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antigens of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured . ^^^ Both t PA and PAI 1 were associated positively with body mass index and blood triglycerides for all sex race groups , and positively with alcohol intake for Japanese and Caucasian men . ^^^ Even when adjusted for body mass index , triglycerides , alcohol and other cardiovascular risk factors , the race difference in mean t PA antigen persisted for men ( P < 0 . 001 ) , as did the difference in mean PAI 1 for men ( P = 0 . 03 ) and women ( P = 0 . 001 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined vascular endothelial cell markers , thrombomodulin ( TM ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) , and von Willebrand factor , in 80 patients with disseminated intravascular coagulation ( DIC ) . ^^^ Plasma TM , PAI 1 , and t PA levels were increased in DIC patients with organ failure or poor outcome , but were not significantly increased before the onset of DIC . ^^^ We consider that the prognosis of patients with DIC might be related to organ failure or endothelial cell damage and that plasma levels of TM , PAI 1 , and t PA might be useful in the detection of these disorders and in assessing prognosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although platelet alpha granules contain plasminogen activator inhibitor 1 ( PAI 1 ) , a fast acting inhibitor of t PA , the contribution of PAI 1 to the antifibrinolytic effect of platelets has remained a subject of controversy . ^^^ Although the incorporation of PAI 1 deficient platelets into clots resulted in a moderate inhibition of t PA mediated fibrinolysis , normal platelets markedly inhibited clot lysis under the same conditions . ^^^ However , no difference between PAI 1 deficient platelets and platelets with normal PAI 1 content was observed when streptokinase or a PAI 1 resistant t PA mutant were used to initiate fibrinolysis . ^^^ In addition , PAI 1 resistant t PA was significantly more efficient in lysing clots containing normal platelets than wild type t PA . ^^^ We conclude that platelets inhibit t PA mediated fibrinolysis by both PAI 1 dependent and PAI 1 independent mechanisms . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , t PA antigen , plasminogen activator inhibitor ( PAI ) , plasminogen ( PL ) and alpha 2 antiplasmin ( AP ) were measured in 10 uremic patients on maintenance hemodialysis treated sequentially with Cuprophane and polyacrylonitrile ( AN 69 ) membranes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A reduced release of t PA was found in 4 patients , while 10 out of 14 patients had comparatively higher levels of plasminogen activator inhibitor ( PAI 1 ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We determined plasma levels of t PA ( activity and antigen ) as well as PAI 1 ( activity and antigen ) in samples obtained before , during and after thrombolytic treatment and compared the course of each of those parameters between responders and non responders to therapy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have measured various fibrinolytic and coagulation parameters ( t PA antigen , PAI , fibrinogen , plasminogen and factor 7 ) before and after 10 min of venous occlusion in 20 hypertryglyceridaemic subjects ( twelve males and eight females , age 38 + / 4 years , body mass index 23 + / 1 . 5 ) and 20 healthy normal subjects , matched for sex ( twelve males and eight females ) , age ( 37 + / 3 . 5 years ) and body mass index ( 22 . 8 + / 1 . 4 ) . ^^^ At rest , t PA : Ag , PAI , fibrinogen , plasminogen and factor 7 were significantly ( P < 0 . 005 ) higher in hypertriglyceridaemic subjects than in normal controls . ^^^ There was a positive correlation between serum triglycerides levels and PAI at rest ( r = 0 . 72 , P < 0 . 01 ) and a negative correlation between serum triglycerides levels and t PA antigen after venous occlusion ( r = 0 . 45 , P < 0 . 05 ) suggesting an impairment of fibrinolysis in hypertriglyceridaemia . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied the plasminogen activation system in tumor tissue by measuring the antigen level of the 2 plasminogen activators , tissue type ( t PA ) and urokinase type ( U PA ) and their inhibitors , plasminogen activator inhibitors type 1 ( PAI 1 ) and type 2 ( PAI 2 ) in the tissue extracts of 43 human benign and malignant ovarian tumors . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of prothrombin fragments 1+2 ( F1+2 ) , tissue plasminogen activator antigen ( t PA ) , plasminogen activator inhibitor 1 activity ( PAI 1 ) , D dimer and interleukin 6 ( IL 6 ) were measured in arterial ( AB ) and mixed venous blood ( MVB ) in five patients during and after total hip replacement operation with acrylic cement prosthesis fixation . ^^^ Sequential peaks of F1+2 , t PA , PAI 1 and IL 6 appeared , starting with activation of coagulation during preparation of bone , closely followed by activation of fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , we evaluated the levels of t PA activity , t PA antigen and plasminogen activator inhibitor 1 ( PAI 1 ) in blood from the patients with central serous chorioretinopathy , and compared them with the levels of total cholesterols ( TCH ) , triglycerides ( TG ) , and high density lipoprotein cholesterol ( HDL ) . t PA activity levels and HDL were lower than in standard controls but t PA antigen , PAI 1 antigen , and TG were higher . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Previous studies have identified plasminogen activator inhibitor type 1 ( PAI 1 ) dependent t PA binding sites in the human hepatoma cell line HepG 2 . ^^^ In this study , we demonstrate that receptor mediated binding and endocytosis of the t PA PAI 1 complex are largely mediated by a recently identified low density lipoprotein receptor related protein ( LRP ) . ^^^ This inhibition by the 39 kDa protein was not due to inhibition of complex formation between 125I t PA and PAI 1 ; instead , the 39 kDa protein inhibited 125I t PA . ^^^ PAI 1 and LRP on HepG 2 cells as anti LRP antibody , in addition to anti t PA and anti PAI 1 antibodies , was able to immunoprecipitate the 125I t PA . ^^^ PAI 1 complex following binding of 125I t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As modulators of fibrinolytic activity , these cells synthesize and secrete tissue plasminogen activator ( t PA ) as well as its physiologic inhibitor , plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Active plasminogen activator inhibitor ( PAI 1 ) antigen , t PA , and urokinase plasminogen activator antigen levels fell significantly after OC treatment and remained low during OC treatment . ^^^ It also did not change the secretion of the fibrinolytic components t PA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples for determination of factor 7 clotting activity using human ( FVIIc ) and bovine thromboplastin ( FVIIbt ) , and for assessment of factor 7 antigen ( FVIIag ) , tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) antigen , PAI activity , t PA and euglobulin fibrinolytic activity , and triglyceride and insulin levels were collected regularly on the second day of each diet period . ^^^ Fibrinolytic variables were not affected by the dietary changes and consistently showed characteristic U shaped ( t PA and PAI 1 antigen , PAI activity ) , or inverted U shaped ( t PA and euglobulin fibrinolytic activity ) circadian patterns with troughs and peaks , respectively , at 17 : 30 21 : 30 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN : Tissue plasminogen activator activity ( t PA ) and antigen ( t PAAg ) and plasminogen activator inhibitor Type 1 antigen ( PAI 1 ) were measured in homogenates of endometrium sampled between 24 and 36 h after the onset of menstruation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both PAI 1 and t PA results were higher in CAPD , probably due to an increased synthesis by endothelial cells activated by glucose peritoneal absorption and hypertonic dialysis solutions . ^^^ The contemporary elevation of fibrinogen , F VIIc , PAI 1 , and t PA suggests that CAPD patients present a hypercoagulability and hypofibrinolysis condition , which may promote the development of atherothrombotic events . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The interaction of endothelin 1 ( ET 1 ) with either interleukin 1 beta ( IL 1 beta ) or tumor necrosis factor alpha ( TNF alpha ) on the release of tissue plasminogen activator antigen ( t PA : Ag ) and plasminogen activator inhibitor 1 antigen ( PAI 1 : Ag ) was investigated in a culture system of vascular endothelial cells derived from human umbilical vein . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
High IRI is accompanied by attenuation of endogenous fibrinolytic activity and increased plasma PAI 1 , the primary physiological inhibitor of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the absence of accessory components , plasminogen activator inhibitor 1 ( PAI 1 ) rapidly forms equimolar , inactive complexes both with tissue type ( t PA ) and with urokinase type ( u PA ) plamsinogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It seems that PGI 2 and ML activated the fibrinolytic system by two independent mechanisms i . e . by a PGI 2 induced direct release of pro fibrinolytic t PA from endothelial cells and by a ML induced suppression of the release of anti fibrinolytic PAI 1 from platelets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic activity ( t PA antigen and activity , PAI 1 antigen and activity ) increased similarly during the adrenaline infusion following ISDN and placebo . 7 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The quantities of t PA Ag and plasminogen activator inhibitor ( PAI ) Ag were determined by using enzyme linked immunosorbent assays . ^^^ We determined t PA and PAI in the aqueous humor of 54 patients between 32 and 87 years of age . ^^^ The t PA Ag levels ranged from 0 . 2 to 1 . 9 ng / ml ( average 0 . 8 ng / ml ) , PAI Ag from 0 . 2 to 1 . 7 ng / ml ( average 0 . 9 ng / ml ) . ^^^ No association between t PA and PAI levels and accompanying diseases or metabolic disorders was noted . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These patients were monitored for t PA antigen and t PA activity and PAI 1 activity plasma levels during rt PA infusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator activity ( PAA ) and plasminogen activator inhibition ( PAI ) , against t PA ( t PAI ) or u PA ( u PAI ) , in spermatozoa and seminal plasma as well as testosterone in the blood of Friesland , Chios , and Karagouniki rams all showed a seasonal variation with the highest values during the corresponding breeding season of the ewes ( Autumn Winter ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Binding of tissue plasminogen activator ( t PA ) by PAI 1 may induce hypofibrinolysis . ^^^ Plasminogen activation was performed by adding plasma to fibrin surfaces to which t PA had been previously bound in the presence of 40 mg / ml bovine serum albumin and 20 mumol / L of the lysine analog trans 4 ( aminomethyl ) cyclohexane carboxylic acid to prevent nonspecific binding and thereby the inhibitory effect of elevated PAI 1 levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The low concentration of plasma PAI 1 was insufficient to neutralize circulating t PA , resulting in high t PA activity with normal antigen and causing the hyperfibrinolytic activity observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The blood was enzymatically analyzed for lactic acid ( LA ) and assayed using chromogenic substrates for the determination of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) , and Factor 8 ( FVIII ) activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lysis of blood clots from vehicle and retinoic acid treated rats could be completely blocked by addition of tranexamic acid or antibodies against rat t PA before clot formation . t PA activity in plasma was slightly increased after retinoic acid treatment ; no effects were measured on plasma PAI 1 , u PA , plasminogen , and alpha 2 antiplasmin levels . t PA activity in lung and kidney was marginally enhanced by retinoic acid but in heart and aortic tissue extracts t PA activity was increased by about 50 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator activity ( PAA ) , t PA antigen level , plasminogen activator inhibition ( PAI ) and plasmin inhibition ( PI ) showed a circadian variation in rat aorta , heart , brain , and lungs of both sexes , but in a different way depending on the organ and the fibrinolytic parameter studied and the sex as well . ^^^ In general , the lowest values of PAA and t PA antigen were found in the early morning and the highest values in late afternoon , while PAI or PI showed the opposite pattern ( with the exception of lungs ) . ^^^ Sex related differences , mostly quantitative , were noted in aorta , heart , brain and kidneys , with lower PAA and t PA antigen and higher PAI or PI in the male than in the female . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this work we have investigated the effects of SDS on the stability of complexes between single chain or two chain tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) . ^^^ Complexes formed by incubation of t PA with PAI 1 for 15 min at 22 degrees C were further incubated with various amounts of SDS before being subjected to SDS polyacrylamide gel electrophoresis . ^^^ The molecular species in the gels were identified both by zymography or by autoradiography after immunoblotting with antibodies directed against either t PA or PAI 1 . ^^^ PAI 1 complexes before electrophoresis resulted in a transition from the complexed state to the free forms of t PA and PAI 1 in a time and dose dependent manner . ^^^ The t PA released from the complexes was fibrinolytically active , whereas the released PAI 1 inhibited activator dependent fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lastly , the simulation predicted that 6 months of endurance training produced a decrease in resting plasminogen activator inhibitor type 1 ( PAI 1 ) secretion , resulting in less t PA inhibition and an overall increase in active t PA after training . ^^^ Accurate analysis of the regulation of active t PA levels in blood required simultaneous modeling of t PA and PAI 1 secretion , hepatic clearance , and inhibition of t PA by PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Their levels of t PA , PAI 1 antigen and platelet factors were compared between A or B , A + B and healthy elderly groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Synthesis of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) was higher in the control cells . ^^^ However , after stimulation with IL 1 , the cells exposed to the dialysate showed greater synthesis of PAI 1 than of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma platelet factor 4 ( PF 4 ) , fibrinopeptide A ( FPA ) , tissue plasminogen activator antigen ( t PA ) , and plasminogen activator inhibitor 1 activity ( PAI 1 ) were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fasting values of serum lipids , plasminogen activator inhibitor ( PAI 1 ) activity , tissue plasminogen activator ( t PA ) antigen , and insulin sensitivity did not differ between Le ( a b ) men and men with other Lewis phenotypes . ^^^ Le ( a b ) women using oral contraceptives ( N = 8 ) had a significantly lower plasma level of fasting PAI 1 activity ( P = . 029 ) and t PA antigen ( P = . 004 ) compared to women using oral contraceptives without the Le ( a b ) phenotype ( N = 42 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma fibrinogen , thrombin antithrombin complex ( TAT ) , plasmin plasmin inhibitor complex , fibrin D dimer , activated protein C ( APC ) protein C inhibitor ( PCI ) complex , von Willebrand factor ( vWf ) , tissue plasminogen activator ( t PA ) , PA inhibitor 1 ( PAI 1 ) , and thrombomodulin levels in both PE and DVT patients were significantly increased compared with normal volunteers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It was found that cadmium markedly decreased the release of both tissue plasminogen activator antigen ( t PA : Ag ) and plasminogen activator inhibitor type 1 antigen ( PAI 1 : Ag ) from vascular smooth muscle cells . ^^^ Other heavy metals including lead , manganese , mercury and nickel also decreased the t PA : Ag and PAI 1 : Ag release , however , cadmium was the most potent inhibitor . ^^^ On the other hand , the release of t PA : Ag was significantly increased whereas that of PAI 1 : Ag was unaffected in fibroblasts after exposure to cadmium . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After one year of practicing Qigong , plasma histofibrinogen activation inhibitor ( PAI ) and 8 factor related antigen ( 8 R : Ag ) levels decreased , while plasma tissue fibrinolytic activator ( t PA ) and anti thrombogen 3 ( AT 3 ) levels increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In order to establish a link between cytokines and the hemostatic changes the following parameters were determined in 44 patients with cirrhosis ( alcoholic = 15 , postnecrotic = 22 , others = 7 ) : TNF alpha , IL 6 , thrombin antithrombin ( TAT ) complexes , prothrombin fragment 1 + 2 ( F 1 + 2 ) and t PA by using enzyme linked immunosorbent assays , and PAI 1 , plasminogen and alpha 2 antiplasmin ( alpha 2 AP ) by using chromogenic substrates . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Active t PA , active PAI 1 and total t PA antigen were measured in plasma samples obtained from 38 subjects , age 32 to 85 ( median 69 years ) , before , during and after CPB . ^^^ Four patterns of fibrinolytic response were noted : 1 ) 40 % of patients showed the `` typical ' ' response , a rapid rise in active and total t PA during CPB followed postoperatively by elevated PAI 1 and reduced t PA , 2 ) 10 % showed no change in t PA or PAI 1 during or after CPB , 3 ) 24 % showed no change in t PA with an increase in PAI 1 postoperatively , and 4 ) 26 % showed an increase in t PA during CPB with no change in PAI 1 postoperatively . ^^^ Age , sex , CPB temperature , total CPB time and preoperative levels of t PA and PAI 1 were not associated in the intra or postoperative fibrinolytic response pattern . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The t PA was in an active form , despite the presence of the inhibitors PAI 1 and PAI 2 . ^^^ The balance of proteases and inhibitors differed dramatically from that observed in plasma , with higher levels of t PA , PAI 1 and PAI 2 , and lower levels of u PA ( urokinase ) , plasminogen , alpha 2 AP and t PA PAI 1 complex in bone marrow , and resulted in favourable conditions for fibrinolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Furthermore both HA and SHA stimulated HUVEC to produce plasminogen activator inhibitor ( PAI 1 ) and tissue plasminogen activator ( t PA ) in a dose and time dependent fashion ; the amount of PAI 1 produced was found to exceed that of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : In pregnant women with GDM ( n = 29 ) which was diagnosed according to WHO criteria the following parameters were examined : number of thrombocytes , APTT , fibrinogen Fbg ( according to Clauss ) , euglobulin fibrinolysis ECLT , t PA concentration , PAI 1 ( Coaliza , Kabi ) and by microturbidimetry the concentration of plasma proteins / orosomucoid ( ORM ) , alpha 1 antitrypsin ( A1AT ) , prealbumin ( PREA ) , transferrin ( TRF ) and alpha 2 macroglobulin ( A2M ) . ^^^ The rise of t PA ( Ag ) , PAI 1 ( Ag ) , fibrinogen and reduction of fibrinolytic activity ( longer ECLT ) made the changes the haemostasis and fibrinolysis in GDM closer to findings in DM type 2 than type 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Treatment of the cells with interleukin ( IL ) beta or tumor necrosis factor alpha ( TNFalpha ) under static conditions had no effect on tissue plasminogen activator ( t PA ) secretion , while release of plasminogen activator inhibitor 1 ( PAI 1 ) increased . ^^^ When cells were exposed to increasing shear stress up to 24 dynes / cm2 , levels of t PA and t PA / PAI 1 complex significantly increased relative to shear stress , while total PAI 1 and active PAI 1 secretion decreased gradually . ^^^ In the presence of IL 1beta or TNFalpha , the increase in production of t PA and the t PA / PAI 1 complex was further augmented . ^^^ Dot blot hybridization analysis of cultured cells in similar experimental conditions using t PA and PAI 1 cDNA probes revealed no t PA mRNA in 3 microg total RNA from static endothelial cells under resting or cytokine stimulated conditions , but abundant t PA mRNA was detected in cells subjected to a shear force of 18 dynes / cm2 , and the increase was further augmented by addition of cytokines . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in a resting and venous occluded state were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fluctuations in tissue plasminogen activator ( t PA ) activity , t PA antigen , and plasminogen activator inhibitor 1 ( PAI 1 ) antigen levels were evaluated in blood samples obtained from 84 patients with initial uneventful acute myocardial infarction ( AMI ) and 35 patients with reinfarction and fatal infarction ( patients with bad prognoses ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased levels of plasma fibrinogen ( FBG ) , coagulation factor 7 ( FVII ) , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) as well as hyperlipidemia are regarded as important risk factors for CVD . ^^^ To investigate whether there are differences in the risk of CVD between chronic hemodialysis ( HD ) and continuous ambulatory peritoneal dialysis ( CAPD ) patients , serum lipid levels and plasma FBG , FVII , t PA , and PAI 1 levels were measured in 17 patients on HD and 17 patients on CAPD . ^^^ FBG was measured by the thrombin time method , FVII activity ( FVIIc ) by the chromogenic prothrombin time method , and t PA and PAI 1 activity by the chromogenic substrate assay . ^^^ T PA showed significant negative correlations with FBG , PAI 1 , TC , LDL C , and TG . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Culture supernatant was assayed for thrombospondin , prostacyclin and PAI 1 and cell lysates were analysed for t PA . aFGF and bFGF were equipotent in regulating ther release of all metabolites studied , except thrombin stimulated release of PGI 2 where bFGF was more potent than aFGF in the absence of heparin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All fibrinolysis markers , that is , D dimer , tissue plasminogen activator antigen ( t PA antigen ) , tissue plasminogen activator activity ( t PA activity ) , plasminogen activator inhibitor activity ( PAI ) and protein C indicated enhanced fibrinolysis by IVRA , but only t PA antigen and PAI showed greater changes in the lignocaine compared with the saline group in the exposed arm at the time of cuff deflation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator antigen ( t PA : Ag ) , plasminogen activator inhibitor type 1 antigen ( PAI 1 : Ag ) and PAI 1 activity , fibrin plate , von Willebrand factor antigen ( vWF : Ag ) , tissue factor ( TF ) and soluble thrombomodulin ( sTM ) were measured . ^^^ The endothelium controls fibrinolysis by producing t PA and its inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
They aimed to examine the effect of proinsulin and insulin on fasting secretion of plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) . ^^^ OCs have a well defined effect on plasma levels of PAI 1 and t PA . ^^^ The clinical researchers also studied the antigen concentrations of PAI 1 and t PA during slow and fast changes in proinsulin and insulin levels induced by oral and intravenous glucose tolerance tests . ^^^ They did not find consistent correlations between fasting values of proinsulin , insulin , PAI 1 , and t PA either before or during OC treatment . ^^^ During the glucose tolerance test induced hyperproinsulinemia and hyperinsulinemia and before OC treatment , PAI 1 and t PA antigen levels fell ( p 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and thrombomodulin were also measured . ^^^ Septic patients with OD showed higher levels of PAI 1 ( P < . 001 ) but not of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The endothelial cells which had not been treated with LPS produced and secreted a large amount of urokinase type PA ( u PA ) , and small amounts of tissue type PA ( t PA ) and PA inhibitor 1 ( PAI 1 ) , which were identified immunohistochemically and by electrophoretic enzymography . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Sequential analyses in a control group with active PTB showed anaemia , thrombocytosis , elevations in plasma fibrinogen , fibrin ( ogen ) degradation products ( FDP ) , tissue plasminogen activator ( t PA ) and inhibitor ( PAI 1 ) with depressed antithrombin 3 levels . ^^^ Age , sex and disease matched individuals with venographically proven DVT had higher FDP ( 15 . 8 + / 14 . 3 5 3 . 2 + / 1 . 7 micrograms / ml : P < 0 . 01 ) , t PA ( 19 . 4 + / 14 . 9 5 11 . 3 + / 0 . 8 ng / ml : P < 0 . 01 ) , and functional PAI 1 activity ( 11 . 6 + / 6 . 3 5 4 . 2 + / 4 . 1 : P < 0 . 01 ) with lower platelet counts ( 347 + / 110 5 563 + / 230 10 10 ( 9 ) / 1 : P < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Moreover , Group 1 and 2 displayed significantly higher levels of factor 7 , fibrinogen , F1+2 , thrombin antithrombin complex and plasminogen activator inhibitor ( PAI ) post venous occlusion and significantly lower levels of tissue plasminogen activator ( t PA ) post venous occlusion than Group 3 . ^^^ Significantly higher levels of t PA and PAI pre venous occlusion and significantly lower levels of antithrombin 3 , C protein and S protein were observed in Group 1 as compared to controls . ^^^ In patients with highest Lp ( a ) level , the lowest t PA level post venous occlusion and the highest PAI level post venous occlusion were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of t PA and t PA PAI 1 complex were relatively low . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESEARCH DESIGN AND METHODS : A total of 135 Caucasian NIDDM subjects treated with oral therapy or diet alone were classified by the presence or absence of retinopathy , and fasting blood samples were taken for assay of PAI 1 antigen and activity , tissue plasminogen activator ( t PA ) , t PA complexed with PAI 1 , euglobulin clot lysis time ( a measure of overall fibrinolytic activity ) , glucose , HbA1c , cholesterol , triglyceride , and insulin levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
O . and 30 AU / ml for PAI 1 in samples taken at rest , there were more patients above those levels in the groups with than without further recurrence ( t PA antigen , 50 % versus 36 % , p = 0 . 001 ; PAI 1 , 18 % versus 12 % , p = 0 . 045 ) . ^^^ In conclusion , increased levels of PAI 1 and t PA antigen in VTE patients correlate with development of recurrent VTE within the next 3 6 years , but the value of these components in predicting future events for the individual patients is limited . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 30 patients with prostatic carcinoma a higher of t PA , a low of PAI 1 , alpha 2 antiplasmin and plasminogen activity with increased fibrin degradation products ( FDP ) level and a short euglobulin lysis time ( ELT ) were observed . ^^^ The activity of t PA and PAI 1 were higher than before operation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Statistically significant variations in the fibrinolytic factors analysed ( tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) were not observed during the menstrual cycle . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Regression analysis revealed associations with anthropometry ( FVII : c , fibrinogen , F1+2 , t PA , PAI 1 ) rather than with age . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Results indicated that urokinase and low dose of t PA significantly reduced the increase of plasminogen activator inhibitor ( PAI ) activity observed 2 h after endotoxin ( p < 0 . 001 ) . ^^^ High t PA dose also diminished the PAI levels at 6 h ( p < 0 . 001 ) . ^^^ In conclusion , treatment with t PA resulted in reduced PAI generation , fibrin deposits and mortality in endotoxin treated rabbits . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Previous studies have reported decreased intrinsic fribrinolytic activity increased systemic plasminogen activator inhibitor 1 ( PAI 1 ) activity and / or decreased systemic tissue plasminogen activator ( t PA ) activity in patients with acute myocardial infarction . ^^^ In each , blood samples were obtained simultaneously from the aorta and coronary sinus for measurement of t PA and PAI 1 activities . ^^^ CONCLUSIONS : Although it has been suggested that alterations in local ( transmyocardial ) t PA and PAI 1 activities may be of pathophysiologic importance in the genesis of unstable angina , our data show no difference in transmyocardial fibrinolytic activity in patients with unstable angina , stable angina , and noncardiac chest pain . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
FbDP did not significantly differ between groups B and C and were significantly lower in group A . t PA antigen and PAI antigen did not significantly differ between the three groups , but t PA activity was elevated and PAI activity and t PA / PAI complexes were reduced in group B . ^^^ PAI activity was significantly reduced in groups A and C . t PA / PAI complexes were significantly enhanced in all cases , but increased to a lower degree in group B . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : All patients had serial coagulation studies including antithrombin ( AT ) 3 activity , prekallikrein , factor 12 , and immunologic tests such as thrombin antithrombin 3 ( TAT ) , fibrinopeptide A ( FPA ) , protein C and S ( PC and PS ) , thrombomodulin ( TM ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , fibrinopeptide B beta 15 42 ( FPB beta 15 42 ) , D dimers , and hemoglobin and platelet counts determined intraoperatively and postoperatively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared with men , women at baseline had similar rhythms for tissue plasminogen activator ( t PA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) activity , but lower levels of total t PA antigen . ^^^ In men ( N = 16 ) , endurance training increased VO2max 15 % ( P < 0 . 001 ) , while decreasing PAI 1 activity 37 % ( P = 0 . 034 ) and total t PA antigen 18 % ( P = 0 . 0003 ) between midnight and 6 a . m . , but did not affect t PA activity . ^^^ In women ( N = 9 ) , endurance training increased VO2max 18 % ( P = 0 . 003 ) , and increased t PA activity 20 % ( P = 0 . 027 ) and total t PA antigen 55 % ( P = 0 . 007 ) between 10 p . m . and 4 a . m . , but had no effect on PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To characterize the vasospastic angina patients with exercise induced ischemia , we measured hemostasis ( platelet factor 4 ; PF 4 , fibrinopeptide A ; FPA ) and fibrinolytic parameters ( tissue plasminogen activator antigen ; t PA , free plasminogen activator inhibitor 1 antigen ; free PAI 1 ) in 15 normal subjects and 33 vasospastic angina patients without significant coronary artery stenosis ( less than 50 % stenosis ) . ^^^ Plasma t PA and free PAI 1 levels in group 1 were significantly higher than those in normal subjects and group 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , the bolus administration of defibrotide in humans was able to induce ( 100 800 mg ) a dose dependent decrease in plasminogen activator inhibitor ( PAI ) ( from 19 . 4 + / 7 . 11 to 7 . 20 + / 6 . 41 AU / mL ) and an increase in t PA ( from 3 . 70 + / 0 . 96 to 4 . 50 + / 1 . 20 IU / mL ) and in the stable prostacyclin derivative 6 keto PGF 1 alpha ( from 18 . 83 + / 3 . 83 to 26 . 75 + / 8 . 48 pg / 0 . 1 mL ) in the venous blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Defibrotide , a polydeoxyribonucleotide , has been found to modulate endothelial cell function , causing an increase in tissue plasminogen activator ( t PA ) levels , a decrease in plasminogen activator inhibitor ( PAI ) levels , and an increase in prostaglandin I 2 ( PGI 2 ) formation in humans . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Differences from normal controls were observed for t PA , PAI 1 , protein C , and protein S ; however , it is not certain that the thrombosis in patients with PAD is due to these factors . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antigen levels of tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitors type 1 ( PAI 1 ) and PAI 2 in GCF were determined with ELISAs and 17 beta oestradiol and progesterone in serum with radioimmunoassays . ^^^ For the total group of women , the concentrations of PAI 2 , PAI 1 and t PA were significantly higher during than after pregnancy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These stimulatory effects on the release of t PA and PAI 1 , but not of u PA , were enhanced by the concomitant addition of progesterone . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and PAI 1 were localized in arterial biopsies of healthy and atherosclerotic vessels by immunohistochemistry . ^^^ Increased expression of t PA , u PA and PAI 1 was found in atherosclerotic vessels . t PA , u PA , PAI 1 and macrophages were co localized in plaques . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Neither tissue plasminogen activator ( t PA ) activity , tissue plasminogen activator ( t PA ) antigen nor plasminogen activator inhibitor ( PAI ) antigen changed during the Mg infusion period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The endothelial cells produced and secreted large amounts of u PA and low levels of tissue type PA ( t PA ) and of PA inhibitor 1 ( PAI 1 ) , which were identified by immunohistochemical study and electrophoretic enzymography . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of thrombin antithrombin ( TAT ) complexes , tissue plasminogen activator ( t PA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) activity were determined in femoral vein blood from both limbs during and after surgery . ^^^ On the operated side , TAT increased during bone traumatization followed by a substantial rise in t PA activity and a gradual decline in PAI 1 activity . ^^^ In an additional study with eight animals undergoing cement anchored hip prosthesis operations the levels of TAT , t PA and PAI 1 were analysed in femoral vein blood , mixed venous blood and arterial blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the present study was to elucidate whether marked job stress affects the parameters of blood coagulation and fibrinolysis , such as plasma fibrinogen concentration , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) activities , in 213 middleaged male workers in a computer producing factory . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The resulting cell line acquired an almost infinite lifespan , was morphologically unchanged , expressed SV 40 antigen , and coexpressed von Willebrand factor ( vWF ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , angiotensin conversion enzyme ( ACE ) , and endothelin converting enzyme ( ECE ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined the interference of endogenous inhibitors such as plasminogen activator inhibitor 1 ( PAI 1 ) and alpha 2 antiplasmin , and that of an endogenous activator namely single chain urokinase type plasminogen activator ( scu PA ) on the Coaset t PA assay . ^^^ The increase in the t PA activity by acidification was found to correlate inversely with PAI 1 ( r = 0 . 66 ; n = 12 ; P = 0 . 019 ) but not with alpha 2 antiplasmin , demonstrating that the extra acidification step released t PA from the t PA PAI 1 complex , but had no influence on the endogenous alpha 2 antiplasmin in the assay system . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The pharmacokinetic and pharmacodynamic data of 100 and 200 mg were analyzed after intravenous injection and of 50 , 100 and 200 mg after subcutaneous injection on tissue factor pathway inhibitor ( TFPI ) antigen and activity , heparin cofactor ( HC ) 2 activity , HeptestTM coagulation value , chromogenic S 2222 anti factor Xa ( aXa ) assay , activated partial thromboplastin time ( APTT ) , thrombin clotting time ( TCT ) , plasminogen , tissue plasminogen activator activity ( t PA ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It has been shown recently in epidemiological studies , that increased concentration of several factors , mainly fibrinogen , factor 7 , von Willebrand factor ( vWF ) , and the fibrinolytic variables plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) , can be considered as risk factors for CHD . ^^^ In fact , it has been shown that the fibrinolytic parameters PAI 1 and t PA antigen are strongly related to the metabolic disorder of insulin resistance , whereas the link with fibrinogen , factor 7 , and vWF remains weak . ^^^ Many cross sectional studies conducted in different populations have shown that PAI 1 and t PA antigen ( which represents t PA / PAI 1 complexes ) are strongly correlated with insulin , triglyceride , high density lipoprotein ( HDL ) cholesterol , body mass index , walst to hip ratio and blood pressure , and that the improvement of insulin resistance improves in parallel the metabolic abnormalities and the concentration of the fibrinolytic parameters . ^^^ Attempts at explaining the elevated PAI 1 and t PA antigen levels in the insulin resistance syndrome have involved many clinical and in vitro studies , in which the role of insulin , insulin propeptides , very low density lipoprotein ( VLDL ) triglyceride , insulin resistance per se , glucose , and adipose tissue have successively been analysed and the main results of these studies are presented in this review . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activation of fibrinolysis by t PA through generation of plasmin is complemented with multifactorial desactivation of platelets by PGI 2 and a selective inhibition of release of plasminogen activator inhibitor 1 ( PAI 1 ) from platelets by NO . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The series consists of 12 subjects ( 6 males , 6 females , mean age 52 + / 7 ) , suffering from acute myocardial infarction ; just after the first symptoms and instrumental signs ( before the thrombolysis ) and after two weeks a venous blood withdrawal was done ; on the plasma of each sample the determination of fibrinogen ( F ) ( coagulative method ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , D dimer ( D D ) , fibrinopeptide A ( FPA ) and betathromboglobulin ( BTG ) ( ELISA methods ) was performed . ^^^ The values of t PA , FPA and BTG did not show remarkable variations ; after two weeks from the myocardial infarction compared to the basal values a significant reduction of PAI 1 ( 4 . 6 + / 0 . 28 UI / ml vs 5 . 4 + / 0 . 33 UI / ml , p < 0 . 01 ) , D D ( 215 + / 10 ng / ml , vs 253 + / 12 ng / ml , p < 0 . 05 ) and a significant increase of F ( 294 + / 28 , vs 218 + / 16 mg % , p < 0 . 05 ) were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On the other hand when fibrin treated sections were incubated with t PA , followed by challenging with Rh PAI 1 , no red fluorescence was observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At the end of each treatment , blood samples for assessment of platelet aggregation and platelet release of platelet factor 4 ( PF 4 ) and for assessment of fibrinolysis , estimated by tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and euglobulin clot lysis time ( ECLT ) , were taken , first at rest , then immediately after five to ten minutes of vigorous exercise , and finally after the subsequent hour of recovery rest . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lipoprotein ( a ) , plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( t PA ) and the PAI 1 / t PA ratio were significantly lower in controls than in coronary , epiaortic and peripheral artery disease patients . ^^^ These results confirm the association between lipoprotein ( a ) , PAI 1 and t PA levels and the presence of atherosclerotic disease independently of the arterial districts considered , while they do not appear to be directly linked to the severity of the morphological disease . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endothelial cells play a central role in fibrinolysis due to their production of both tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ The purpose of this study was to test the hypothesis that the production of t PA and PAI 1 from human umbilical vein endothelial cells ( HUVEC ) and from human adult vein endothelial cells ( HAVEC ) adopt the same pathways for the regulation of fibrinolysis , and that differences in PAI 1 and t PA production are only quantitative . ^^^ The conditioned medium was collected after 16 h and analyzed for t PA and PAI 1 antigens . ^^^ The basal production of both t PA and PAI 1 was significantly higher from HUVEC than from HAVEC . ^^^ The results indicate that there are not only quantitative but also qualitative differences in the production of t PA and PAI 1 from HUVEC and HAVEC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Sonographic IAD correlated positively with plasma fibrinogen ( r = 0 . 33 ; P < 0 . 01 ) , PAI 1 activity ( r = 0 . 52 ; P < 0 . 0001 ) and antigen ( r = 0 . 32 ; P < 0 . 05 ) , and negatively with t PA activity , at baseline and after stasis ( r = 0 . 28 and r = 0 . 31 ; P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 activity , PAI 1 antigen and tissue plasminogen activator ( t PA ) antigen levels decreased during the GH treatment period ( p < 0 . 05 ) . ^^^ In conclusion , PAI 1 activity , PAI 1 antigen and t PA antigen decreased during long term GH treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Alterations in the plasma levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) as observed in various liver disorders are attributed to deranged liver function . ^^^ Three patients each showed mildy increased levels of t PA ( 3 / 27 ) and PAI 1 ( 3 / 27 ) . ^^^ The levels of t PA and PAI 1 however did not show any correlation with liver function parameters . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study , u PA , t PA and PA activator 1 ( PAI 1 ) antigen and activity were measured in plasma and pleural fluid samples from patients with MPM , lung cancer and benign effusion . ^^^ These findings were in contrast to the lung cancer group , in which both activity and immunologic measurement of u PA and t PA were higher , but PAI 1 antigen was similar as compared to the control group . ^^^ It is concluded that excess t PA and u PA are balanced in complexes with PAI 1 in MPM , whereas the amount of PAI 1 in plasma is insufficient to overcome the elevated t PA and u PA , in lung cancer . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lp ( a ) modulated the thromboresistant cell surface by reduction of t PA and u PA , but PAI 1 remained unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Localization of t PA , u PA , PAI 1 , PI and TGF beta within tumors was examined immunohistologically in 31 patients with squamous cell carcinoma ( SCC ) of the head and neck , and correlations between the localization of these factors and local cancer infiltration , tumor size or cervical lymph node metastasis were investigated . ^^^ The results revealed that u PA , PAI 1 and PI tend to stain more intensely in infiltrating tumors than in peripheral connective tissue or normal epithelium , whereas neither t PA nor TGF beta showed any such tendency . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Pearson and Spearman correlation coefficients were calculated to determine associations between the variables of interest and tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activities in the basal state and after stimulation with maximal exercise . ^^^ Maximal exercise produced significant increases in t PA activity and decreases in PAI 1 activity . ^^^ Postexercise t PA activity was inversely related to basal PAI 1 activity ( r = . 34 ) . ^^^ Vo2max was positively correlated with t PA activity ( basal , r = . 39 ; postexercise , r = . 67 ) and inversely related to PAI 1 activity ( basal , r = . 41 ; postexercise , r = . 42 ) . ^^^ Body fat was correlated with postexercise t PA activity ( r = . 60 ) and both basal and postexercise PAI 1 activity ( r = . 42 ) , but the correlation with basal t PA activity was not significant ( P = . 058 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma fibrinolytic potential is reduced in NIDDM and it is known that glucose and insulin can modulate plasminogen activator inhibitor ( PAI 1 ) and tissue plasminogen activator ( t PA ) secretion and can therefore regulate local fibrinolysis . ^^^ Vascular smooth muscle cells ( vSMC ) play an important role in the development of atherosclerotic lesions ; however , the role of insulin and glucose in regulating PAI 1 and t PA production in vSMC is presently not known . ^^^ After 24 h , PAI 1 and t PA antigens and activity were evaluated in the culture medium ; in cells exposed to 20 mmol / l glucose and to 0 . 5 nmol / l insulin PAI 1 gene expression was also evaluated . ^^^ An increase in PAI 1 antigen was observed at each glucose concentration ( by 138 , 169 , 251 and 357 % as compared to 5 mmol / l glucose ) which was paralleled by an increase in PAI 1 activity . t PA concentration was also increased by glucose but its activity was sharply reduced . ^^^ PAI 1 activity was slightly increased at the lowest insulin concentrations but markedly increased by 10 nmol / l insulin . t PA antigen was also increased by insulin ; however , its activity was markedly reduced at each concentration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( T PA ) and tissue plasminogen activator inhibitor ( PAI 1 ) in patients after head injury ] . ^^^ The following factors of fibrinolysis : tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor 1 ( PAI 1 ) play an important role in patients after trauma . ^^^ We measured changes of the level of t PA antigen and PAI 1 activity in days 0 3 , 4 6 and later . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coagulation was explored by standard clotting tests and by measurement of prothrombin complex factors , factor 8 ( 8 : C ) and antithrombin 3 ( ATIII ) , protein C ( PC ) and protein S ( PS ) activities , while fibrinolytic potential was evaluated using D dimer , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) assays . ^^^ In all SCD patients , baseline levels of t PA antigen were comparable to those in controls , whereas concentrations of PAI 1 antigen were significantly increased , either in the steady state ( 89 . 7 + / 26 . 3 ng / ml , p < 0 . 0001 ) or in crisis ( 75 . 0 + / 24 . 8 ng / ml , p < 0 . 0001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Different parameters of fibrinolytic systems like t PA , PAI , D dimer , and inhibitors of blood coagulation , i . e . , protein C ( PC ) , protein S ( PS ) , and antithrombin 3 ( AT 3 ) , have been studied in cases of acute malaria due to Plasmodium falciparum and plasmodium vivax infection , and these patients were followed up . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Mean baseline plasma PAI activity ( P < 0 . 01 ) and t PA antigen ( P < 0 . 01 ) levels were higher in the patient group than they were in the control group . ^^^ At the conclusion of EPA administration , significant reductions in PAI activity ( P < 0 . 01 ) , t PA antigen ( P < 0 . 01 ) and serum levels of triglyceride ( P < 0 . 01 ) , total ( P < 0 . 05 ) and low density lipoprotein ( P < 0 . 05 ) cholesterols were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This seemed to be due to increased synthesis of its inhibitor , plasminogen activator inhibitor 1 , under these conditions rather than to decreased expression of the t PA enzyme . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : The purpose of the examination was evaluation of tissue plasminogen activator ( t PA ) concentration and plasminogen activator inhibitor ( PAI ) activity in aqueous humor of the eye and plasma of blood in patients operated on cataract . ^^^ MATERIAL AND METHODS : In aqueous humor and citrate plasma of 24 patients undergoing cataract surgery the concentration of t PA ( Imulyse Biopool ) and the activity of PAI 1 ( Spectrolyse Biopool ) were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Post mortem concentration of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) in the vitreous body of the human eye ] . ^^^ Earlier we observed after sudden death high concentrations of t PA Ag in plasma with strong activation of fibrinolysis , consumption of PAI 1 , plasminogen , fibrinogen , alfa 2 antiplasmin and a big increase of FDP . ^^^ Fibrinolysis is mainly regulated by t PA , u PA and PAI 1 . ^^^ PURPOSE : The aim of our study was the evaluation of t PA and PAI 1 in corpus vitreous and plasma of patients after sudden ( 26 ) and late death ( 12 ) . ^^^ Material and methods : The concentration of t PA Ag was measured with COA SET of Kabi Vitrum and the activity of PAI 1 with reagents of Biopool . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic system was investigated through the plasma levels of antigenic and functional t PA and PAI 1 . ^^^ RESULTS : The antigenic levels of t PA and PAI 1 , were significantly higher in the DCM group than in the control group ( p < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Quantitative measurement of plasma concentrations of tissue t PA , t PA PAI 1 complex , alpha 2 antiplasmin plasmin complex ( alpha 2 APP ) , fibrinogen degradation products ( FbDP ) , D dimers ( D D ) , thrombin antithrombin complex ( TAT ) and prothrombin fragment ( F 1 + 2 ) complexes , using double antibody sandwich enzyme linked immunosorbent assays and grading of the degree of hepatic insufficiency according to the Child Pugh classification , were also carried out . ^^^ RESULTS : The progressive deterioration of liver function in schistosomal patients , which matched the severity of the disease , led to simultaneous defects in profibrinolytic ( decreased Plg and increased PA and t PA ) and antifibrinolytic ( decreased alpha 2 AP and PAI 1 ) factors the latter defects being the most prominent resulting in significant generation of plasmin ( increased APP complexes ) and therefore enhanced fibrinolysis ( increased FbDP and D dimer ) . ^^^ CONCLUSION : These findings suggest that the amount of PAI 1 available to bind and neutralise circulating t PA may be a critical factor in the progress of hyperfibrinolysis observed in hepatosplenic schistosomiasis , and that the pronounced reduction in its plasma concentration may be regarded as a potential warning indicator of haemostatic imbalance in decompensated schistosomal patients at high risk of variceal bleeding . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effects of nicotine treatment ( 4 . 5 mg / kg of nicotine free base / day administered s . c . by osmotic minipumps for 14 days ) on focal ischemic stroke and expression of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in cerebral microvessels were studied in rats in vivo using a reversible ( 1 h ) middle cerebral artery occlusion model . ^^^ A free pool of brain microvascular t PA antigen was completely depleted by nicotine , while the expression of the PAI 1 antigen and / or PAI 1 t PA complexes remained unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here , we report negative feedback regulation of t PA expression , exerted by t PA or complexes of t PA with its physiological inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ PAI 1 complexes and reversed by monoclonal antibody directed against the active site of t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator antigen ( t PA : Ag ) and plasminogen activator inhibitor 1 antigen ( PAI 1 : Ag ) released were determined by enzyme immunoassay . ^^^ It was found that lead decreased the release of both t PA : Ag and PAI 1 : Ag from vascular smooth muscle cells . ^^^ On the other hand , in fibroblasts , the release of t PA : Ag was markedly decreased whereas that of PAI 1 : Ag was markedly increased by the metal . ^^^ However , the effects of lead on the release of t PA : Ag and PAI 1 : Ag were different from those of calcium ionophore A 23187 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Inflammation or abrasion of the mesothelium during surgery is likely to cause a depletion of the local t PA source and expose the potentially PAI 1 containing submesothelial tissue , thus promoting persistence of fibrin and formation of adhesions . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These in vivo data suggest that infusion of bradykinin results in an increase in circulating t PA levels without an effect on PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Differences were also observed for tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) with MVEC supernatants or cell lysates . ^^^ The fibrinolytic potential , expressed as t PA / PAI 1 ratio , was lower in ARDS than in lung MVEC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PGI ( 2 ) , von Willebrand Factor ( vWF ) , t PA and PAI 1 were measured to evaluate changes in cell function induced by modified fibrin structure . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The 6 hours conditioned medium obtained from the coculture system between monocytes and either ECs or paraformaldehyde fixed ECs had almost the same effect on the other ECs to produce PAI 1 and t PA as monocytes that were direct contact with ECs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All patients had serial coagulation studies , including prothrombin split products ( F1 / F2 ) , fibrinopeptide A ( FPA ) , protein C ( PC ) , thrombomodulin ( TM ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , fibrinopeptide B beta 15 42 ( FPB beta 15 42 ) , haemoglobin and platelet counts determined intra and postoperatively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor ( PAI ) , using COA SET , t PA and COA TEST PAI ( Chromogenix ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Progressively aggressive neoplastic cells evidence high expression of u PA and u PAR activities , variable expression of t PA , and enhanced PAI 1 and PAI 2 activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Modification of the RAS via ACE inhibition may protect against thrombosis by limiting vascular expression of PAI 1 and augmenting bradykinin induced production of t PA . ^^^ Survivors of myocardial infarction treated with an ACE inhibitor have demonstrated a reduction in PAI 1 activity and preservation of the normal ratio of PAI 1 to t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Baseline plasma levels of t PA , plasminogen activator inhibitor 1 ( PAI 1 ) , plasminogen , alpha 2 antiplasmin , fibrinogen , lipoprotein ( a ) , thrombomodulin and von Willebrand factor as well as platelet and leukocyte count and clot retraction were also determined in each donor . rt PA induced clot lysis varied over a wide range ( 28 75 % ) and was significantly related to endogenous t PA , PAI 1 , plasminogen ( p < 0 . 001 ) and age ( p < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , shear stresses of 6 approximately 24 dynes / cm2 decreased the expression of TNF alpha induced TF in a shear intensity and exposure time dependent manner Tissue plasminogen activator ( t PA ) , which converts plasminogen to plasmin to degrade fibrin clot , and plasminogen activator inhibitor 1 ( PAI 1 ) , which inhibits t PA function , play central roles in fibrinolysis in the endothelium . ^^^ Treatment of the cells with IL 1 beta or TNF alpha under static conditions had no effect on t PA secretion , while release of PAI 1 increased . ^^^ When cells were exposed to increasing shear stress up to 24 dynes / cm2 , levels of t PA significantly increased relative to shear stress , while PAI 1 secretion decreased gradually . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine whether the ACE inhibitor captopril reduces plasma PAI inhibitor activity , we measured changes in plasma PAI activity ( IU / ml ) , tissue plasminogen activator ( t PA ) antigen ( ng / ml ) , and serum ACE activity ( IU / L ) in 14 survivors of myocardial infarction receiving captopril therapy ( 37 . 5 mg daily ) and compared them with the values in 15 placebo treated patients chosen at random . ^^^ In the captopril treated group , serum ACE activity decreased significantly , from 14 . 0 + / 0 . 8 to 11 . 5 + / 1 . 2 IU / L 24 h after captopril therapy ( p < 0 . 01 ) , and those of PAI activity and t PA antigen also decreased significantly from 11 . 9 + / 2 . 8 to 5 . 5 + / 2 . 2 IU / ml ( p < 0 . 02 ) and from 9 . 9 + / 1 . 0 to 7 . 5 + / 0 . 9 ng / ml ( p < 0 . 05 ) , respectively 48 h after captopril therapy . ^^^ However , the levels of ACE activity , PAI activity , and t PA antigen remained unchanged during the study period in the placebo group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , thrombomodulin ( TM ) , von Willebrand factor ( vWF ) , factor 7 , fibrinogen ( FBG ) , FDP , D dimer ( D D ) , plasminogen ( PLG ) , thrombin antithrombin 3 ( TAT ) , alpha 2 plasmin inhibitor / plasmin complex ( PIC ) , antithrombin 3 ( AT 3 ) , protein C , N terminal propeptide for type 3 procollagen ( P 3 P ) , were measured weekly from pre BMT to day 28 after BMT . ^^^ In VOD patients , t PA and PAI 1 significantly increased ( P < 0 . 05 ) and FBG significantly fell during the post transplant period ( P < 0 . 05 ) . ^^^ There were no changes in other hemostatic parameters . t PA , PAI 1 and FBG would thus appear useful markers for the diagnosis of VOD , and AT 3 and PLG , predictive markers for VOD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of work was to study whether activity and antigen level of tissue plasminogen activator ( t PA ) and activity of plasminogen activator inhibitor ( PAI 1 ) were related to severity and extensiveness of vascular lesions in peripheral obliterative atherosclerotic disease ( POAD ) . ^^^ In the blood t PA activity and antigen and activity of PAI 1 were determined . ^^^ In the patients with more severe POAD higher t PA antigen and increased t PA complexed with PAI 1 were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serial levels of fibrinopeptide A ( FPA ) , fibrinopeptide B beta 15 42 ( FPB beta 15 42 ) , D dimer , tissue plasminogen activator antigen concentration ( t PA antigen ) , t PA activity , plasminogen activator inhibitor 1 antigen concentration ( PAI 1 antigen ) , and PAI 1 activity were determined in 63 patients with out of hospital cardiopulmonary arrest . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our data show that ( 1 ) a pneumatic compression device that exerts its compression on the plantar venous plexus only induced an increase of venous blood peak velocity and flow in the common femoral vein that is very similar to that induced by the classical whole leg boots compression system ; ( 2 ) the venous stasis induced by an external pressure mimicking the conditions of laparoscopic surgery further increased the absolute velocity and flow increase , with the two intermittent compression systems tested ; ( 3 ) no changes of t PA or plasminogen activator inhibitor 1 antigens were observed with either pneumatic compression device . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Citrated blood samples were taken from each patient the day before surgery and on postoperative days 1 , 3 , and 7 , before and after venous occlusion , in order to evaluate plasma levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
STUDY DESIGN : One hundred and seventeen normal pregnant women were investigated in a longitudinal study involving five measurements : blood samples were collected at 10 , 20 , 30 , 36 weeks and on the second day puerperium and were assayed for prothrombin time ( PT expressed in INR ) , activated partial thromboplastin time ( PTT ) , fibrinogen ( FBG ) , antithrombin 3 activity ( AT 3 ) , protein C activity ( PC ) , protein S activity ( PS ) , prothrombin fragments 1+2 ( F1+2 ) , type 1 plasminogen activator inhibitor activity ( PAI ) and tissue plasminogen activator antigen ( t PA ) . ^^^ With regard to fibrinolysis , there was an increase both for t PA ( P < 0 . 0001 ) and PAI 1 ( P < 0 . 0001 ) during pregnancy . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The principal effect of IL 6 is a more rapid normalization of overall coagulation tests , due to normalization of fibrinogen , and an increased t PA release response which is partially counteracted by increased PAI 1 concentrations . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We report the effect of IL 1 and transforming growth factor beta ( TGF beta ) and a combination of the two cytokines on the secretion of tissue type PA ( t PA ) and PAI 1 from human mesangial cells . ^^^ IL 1 significantly downregulates PAI 1 production and upregulates t PA over a range of concentrations ( 1 10 U / ml ) , while TGF beta ( 0 . 5 10 ng / ml ) has the opposite effect . ^^^ The combined effect of these factors is that TGF beta attenuates the increase in t PA and decrease in PAI 1 expression caused by IL 1 , and has a dominant effect upon their secretion , thus decreasing t PA and increasing PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Some coagulative and fibrinolytic parameters ( PT ; aPTT ; fibrinogen ; euglobulin lysis time ; t PA ; PAI 1 ; ATIII ; alpha 2 antiplasmin ; D Dimer and platelets count ) were assayed at the beginning and at the end of the study . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Studies on the effects of flavonoids isolated from the roots of Scutellaria baicalensis on the fibrinolytic system induced by trypsin in cultured human umbilical vein endothelial cells ( HUVECs ) showed that baicalein ( 1 ) strongly inhibited the reduction of t PA production and the elevation of PAI 1 production induced by trypsin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : For this reason we have studied plasma lipid concentrations ( C , TG , HDL C ) , Lp ( a ) and some parameters of haemostasis ( PAI 1 , t PA , D dimer ) in 41 non diabetic obese patients ( 38 females and 3 males ) and in 36 healthy normal weight subjects . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MEASUREMENTS : ( 1 ) plasma concentrations of plasminogen activator inhibitor 1 antigen ( PAI 1 Ag ) , plasminogen activator inhibitor 1 activity ( PAI 1 activity ) , fibrinogen , von Willebrand factor antigen ( vWF Ag ) , von Willebrand factor activity ( vWF activity ) , and factor 7 activity as pro thrombotic factors ; ( 2 ) plasma concentrations of tissue plasminogen activator antigen ( t PA Ag ) , protein C , and antithrombin 3 as anti thrombotic factors ; ( 3 ) fasting plasma insulin and glucose concentrations , and the lipid pattern ( triglycerides , total and HDL cholesterol ) as metabolic parameters . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , von Willebrand Factor ( vWF ) , and fibrin D dimer were determined by enzyme linked immunosorbent assays . ^^^ Patients with transplant coronary artery disease had higher circulating t PA ( 8 . 6 + / 0 . 8 vs . 5 . 4 + / 0 . 6 ng / ml , p = 0 . 021 ) and PAI 1 antigen concentrations ( 38 . 0 + / 3 . 4 vs 25 . 8 + / 2 . 2 ng / ml , p = 0 . 037 ) . t PA and PAI 1 antigen concentrations correlated with the severity of angiographic disease ( R = 0 . 34 ; p = 0 . 014 for t PA , and R = 0 . 45 ; p = 0 . 001 for PAI 1 ) . ^^^ We conclude that cardiac transplant patients with evidence of transplant coronary artery disease on coronary angiography have altered hemostatic function which is reflected by elevated levels of circulating t PA and PAI 1 antigens . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In twenty patients with primitive venous hypertension and in ten healthy subjects we have determined the plasmatic levels of : Thrombomodulin ( TM ) , beta Thromboglobulin ( beta TG ) , D Dimer ( DD ) , the tissue activator of the plasminogen ( t PA ) and the inhibitor of the activator of the plasminogen ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared with healthy brothers or sisters and a healthy age matched control group , thrombin generation , D dimer , PAI 1 activity , and t PA antigen were found clearly elevated in children with APCR . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All subjects were tested on the following parameters : fibrinogen , factor 7 , prothrombin fragment 1 + 2 ( F 1 + 2 ) , thrombin antithrombin 3 complexes ( TAT ) , tissue plasminogen activator ( t PA ) antigen ( Ag ) before and after venous occlusion ( VO ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) activity pre and post VO . ^^^ At baseline , obese nondiabetic subjects , lean NIDDM patients , and especially obese NIDDM patients displayed significantly ( P < . 01 ) higher levels of fibrinogen , factor 7 , F 1 + 2 , TAT , t PA ( Ag ) pre VO , and PAI 1 pre and post VO and significantly ( P < . 01 ) lower levels of t PA ( Ag ) post VO . ^^^ In obese NIDDM patients treated with heparin fibrinogen , factor 7 , F 1 + 2 , TAT , t PA ( Ag ) pre VO , and PAI 1 pre and post VO levels significantly ( P < . 01 ) decreased and t PA ( Ag ) post VO levels significantly ( P < . 01 ) increased at the end of treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In a controlled intervention study among elderly subjects ( aged 60 80 years ) we observed a moderate decrease in PAI 1 antigen ( 4 % , 2 . 1 + / 2 . 4 ng / ml ) , a significant increase in t PA activity ( 11 % , 0 . 07 + / 0 . 04 IU / ml ) and an unexpected significant increase in fibrinogen ( 6 % , 0 . 18 + / 0 . 07 g / l ) in subjects following a 6 month intensive training program as compared to controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Quantitative reverse transcription polymerase chain reaction and in situ hybridization were employed to investigate the expression of tissue type and urokinase type plasminogen activators ( t PA and u PA , respectively ) , of their specific inhibitor ( PAI 1 ) , and of the procoagulant molecule tissue factor ( TF ) in tissues from mice that develop autoimmune disease ( MRL lpr / lpr ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The trandolapril group did not differ significantly from the placebo group with respect to AUC 1 12 t PA antigen [ 11 . 67 ( 3 . 95 26 . 45 ) versus 10 . 34 ng / ml ( 3 . 71 19 . 62 ) , P = 0 . 19 ] and AUC 1 12 plasminogen activator inhibitor type 1 ( PAI 1 ) antigen [ 27 . 57 ( 8 . 38 89 . 49 ) versus 24 . 40 ng / ml ( 7 . 94 90 . 62 ) , P = 0 . 92 ] . ^^^ Trandolapril treatment was associated with a significant ( P < 0 . 04 ) increase in the AUC 1 12 of t PA antigen in the ID group compared with that of the placebo treated ID group , whereas PAI 1 antigen concentration did not differ between the groups . ^^^ CONCLUSIONS : Chronic ACE inhibitor treatment with trandolapril was not associated with any significant difference in the blood concentrations of t PA and PAI 1 compared with placebo . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Aortic SMC were isolated from transgenic mice showing single inactivations of the t PA , u PA , plasminogen activator inhibitor 1 , or urokinase type plasminogen activator receptor ( u PAR ) genes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of study was the evaluation of tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) in the 2 type diabetes on the base of the common fibrinolytic system tests as euglobulin lysis time ( ELT ) and the concentration of fibrin / fibrinogen products ( FDP ) . ^^^ In the blood the following determinations were performed : antigen of t PA and PAI 1 , ELT and FDP . ^^^ In the blood of patients with 2 type diabetes increased . t PA Ag and PAI 1 Ag concentrations were observed . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although initially tissue plasminogen activator ( t PA ) activity was lower in the aprotinin than placebo group , as surgery proceeded this difference was reversed due to less plasminogen activator inhibitor 1 release in the aprotinin group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A significantly lower PAI 1 antigen decrease along with a lower t PA antigen release was found in the APC resistant children compared with the controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study we report the effects of angiotensin 2 on the production of PA inhibitor 1 ( PAI 1 ) and tissue type PA ( t PA ) by glomerular mesangial cells in culture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma PAI 1 levels appeared to depend on the genotype ( P = . 014 ) as much as on age ( P = . 044 ) , t PA ( P = . 0001 ) , or triglyceride levels ( P = . 005 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The major physiologic inhibitor of t PA and u PA is plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In all individuals , the euglobulin clot lysis time and plasma antigen levels of t Pa and PAI 1 were measured before and after venous occlusion ( v . o ) for 10 min . ^^^ Among the latter , 22 patients had significantly increased baseline levels of PAI 1 Ag ( 82 . 6 + / 27 . 5 ng / ml , p < 0 . 001 ) and a normal release of t Pa Ag after v . o . ^^^ In contrast , 11 patients had basal values of PAI 1 Ag comparable to those in controls with a defective release of t Pa Ag after v . o ( 11 . 4 + / 5 . 2 ng / ml , p < 0 . 01 ) . ^^^ These data provide evidence for reduced fibrinolytic capacity resulting from either increased basal levels of PAI 1 or defective release of t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The oral estrogen group was associated with a significant decrease in both mean tissue type plasminogen ( t PA ) concentration and plasminogen activator inhibitor ( PAI 1 ) activity and a significant rise in global fibrinolytic capacity ( GFC ) compared with the two other groups . ^^^ A transdermal estrogen regimen had no significant effect on PAI 1 , t PA , and GFC levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A fourfold molar excess in PAI 1 : t PA was seen in conditioned media from samples with any evidence of atherosclerosis compared with normal specimens ( normal 21 + / 4 , diseased 82 + / 21 , P < or = . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Subjects in the highest LDL 6 apoB tertile had higher PAI 1 ( 24 . 7 vs . 13 . 1 AU / ml , p < 0 . 001 ) and lower t PA ( 0 . 26 vs . 0 . 54 IU / ml , p < 0 . 001 ) activities than men in the lowest tertile . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study we have analyzed the relationship between coagulation inhibitors ( antithrombin 3 , protein C and S , thrombomodulin ) , liver function impairment , and plasma activity of the endothelium derived proteins plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) in 27 alcoholic cirrhotic patients and 25 controls . ^^^ Cirrhotics showed decreased values of all the mentioned parameters except for thrombomodulin , PAI 1 , and t PA . ^^^ No relationship was observed between thrombomodulin and t PA or PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coagulative and fibrinolytic parameters ( PT , aPTT , euglobulin lysis time , fibrinogen , D dimer , t PA , PAI 1 ) and signs and symptoms ( cramps , paresthesia , itch , edema , local pain , skin trophism ) were assessed at enrollment , 15 days later after the pharmacological washout period and after 1 , 2 , 3 months of treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following factors were measured : tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasmin alpha 2 plasmin inhibitor complex ( PIC ) , stabilized fibrin degradation product ( D dimer ) , and von Willebrand factor antigen determined by enzyme immunoassay , and endothelin 1 by radioimmunoassay with two antibodies . ^^^ There was no correlation between percent and absolute changes during the occlusion in endothelin 1 and those in t PA , PAI 1 , PIC , D dimer , or von Willebrand factor . ^^^ The mechanism by which endothelin 1 is released in response to the occlusion appears to differ from that for t PA , PAI 1 , and von Willebrand factor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All doses of r hirudin prevented decreases in tissue plasminogen activator ( t PA ) and reduced the increase in plasminogen activator inhibitor 1 ( PAI 1 ) activity observed at 2 hours after endotoxin administration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) play important role in regulation of fibrinolysis . ^^^ In the blood of patients we measured the concentration of t PA : Ag , fibrinogen and FDP levels , PAI 1 activity , and ELT . ^^^ The increase of t PA and FDP concentrations , decrease of PAI 1 activity and fibrinogen level with simultaneous ELT shortening indicate on the fibrinolysis activation during the laryngectomy . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue and urokinase plasminogen activators ( t PA , u PA ) and their inhibitor PAI 1 in blood of patients with laryngeal neoplasms ] . ^^^ In this study we measured the concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , activity of plasminogen activator inhibitor type 1 ( PAI 1 ) and euglobulin lysis time ( ELT ) in the blood of 20 men aged 42 75 years old with planoepitheliale larynx carcinoma , and 10 healthy persons in similar age . ^^^ In this study the mean values of t PA , u PA concentrations , PAI 1 activity and ELT in the blood of patients with larynx carcinoma were similar to the examination results of healthy persons , but 40 % of our cancer patients have increased activity of PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate the relationship between coronary artery disease , activated factor 12 and other circulating factors , we studied levels of FXIIa , cholesterol , triglycerides , fasting insulin , fibrinogen , FVII : C , t PA antigen and PAI 1 antigen . ^^^ In patients , FXIIa levels positively correlated with FVII : C , BMI , cholesterol , insulin , PAI 1 antigen , t PA antigen and triglycerides . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The quantities of t PA and plasminogen activator inhibitor ( PAI ) were determined using enzyme linked immunosorbent assays . ^^^ We determined t PA and PAI in the aqueous humor of 64 patients between 59 and 82 years of age . ^^^ RESULTS : The t PA levels ranged from 0 . 65 to 1 . 75 ng / ml and PAI activity from 3 . 24 to 5 . 1 AU / ml . ^^^ Association between t PA levels and PAI activity and accompanying diseases or metabolic disorders was noted . ^^^ The highest concentration of t PA and the lowest activity of PAI has been observed in aqueous humor of patients with senile cataract . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombin antithrombin 3 complex ( TAT ) , D dimer , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) 1 antigens ( Ag ) , t PA activity , and plasmin alpha 2 antiplasmin complex ( PAP ) , as well as plasma catecholamines , were measured before and after a treadmill exercise test . ^^^ At rest , fibrinogen ( 3 . 3+ / 0 . 5 versus 2 . 9+ / 0 . 5 g / L [ mean+ / SD ] ; P < . 05 ) , D dimer ( 392+ / 128 versus 271+ / 113 ng / mL ; P < . 05 ) , t PA Ag ( 9 . 1+ / 5 . 1 versus 5 . 5+ / 1 . 2 ng / mL ; P < . 02 ) , and PAI 1 Ag ( 14 . 9+ / 7 . 1 versus 7 . 6+ / 3 . 8 ng / mL ; P < . 002 ) levels in plasma were markedly higher in the patient group than in the control group . ^^^ Exercise caused a parallel increase in D dimer , t PA Ag , t PA activity , PAP , and catecholamines in both groups , whereas PAI 1 Ag remained stable . ^^^ Compared with healthy control subjects , patients with PAOD showed higher t PA Ag , PAI 1 Ag , and D dimer levels both at rest and after exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The two fucosylated FG 2 Fc chimeras were compared with each other , with non fucosylated FG and with intact t PA with regard to their effect on the clearance of t PA and t PA 10 plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ In rat hepatoma cells and in mouse embryonic fibroblasts ( MEF 1 ) the clearance of t PA and of t PA 10 PAI 1 was inhibited more than 95 % by receptor associated protein , an inhibitor of LRP mediated clearance , whereas no t PA or t PA 10 PAI 1 clearance was observed in LRP deficient PEA 13 mouse embryonic fibroblasts . ^^^ Their efficacies in inhibiting t PA and t PA 10 PAI 1 degradation ( IC 50 750 nM and 890 nM , respectively ) were similar to those of non fucosylated FG ( IC 50 1950 nM and 1560 nM ) and 75 fold lower than that of intact t PA ( IC 50 9 . 9 nM and 21 . 1 nM ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Enhanced invasion is associated with increased u PA , UPAR , PAI 1 , MT MMP 1 , MMP 9 and TIMP 1 expression ; with reduced t PA , MMP 1 and MMP 3 expression ; and with the induction of membrane MMP 9 association . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the acute phase , intense plasminogen activator inhibitor 1 ( PAI 1 ) gene expression was apparent in areas interfacing the dissecting hematoma , but no tissue type PA ( t PA ) , urokinase type PA ( u PA ) , or MMP 9 mRNAs were detected . ^^^ Although PAI 1 mRNA was still present in the subacute phase , t PA , u PA , and MMP 9 mRNAs were now obvious , with PA gene expression co localizing with areas of PAI 1 gene expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Both on day 0 and day 10 , patients with an acute coronary syndrome had a marked elevation of t PA mass concentration ( mean value + / SEM 14 . 4 + / 1 . 6 [ day 0 ] , 18 . 9 + / 2 . 5 ng / ml [ day 10 ] ) and of plasminogen activator inhibitor ( PAI ) ( 9 . 4 + / 2 . 2 [ day 0 ] , 11 . 3 + / 2 . 6 AU / liter [ day 10 ] , p < 0 . 05 vs . patients with stable angina ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antithrombin 3 ( ATIII ) , thrombin antithrombin 3 complexes ( TAT ) , heparin cofactor 2 ( HCII ) , protein C ( PC ) , protein S ( PS ) , tissue plasminogen activator ( t PA ) , D dimer , and plasminogen activator inhibitors ( PAI 1 and PAI 2 ) levels in uncomplicated pregnancies and in pregnancies complicated by intrauterine growth retardation ( IUGR ) have been determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recent evidence is accumulating that some clotting factors ( fibrinogen , factor 7 , von Willebrand factor ) and fibrinolytic factors ( t PA and PAI 1 ) are associated with an increased risk of coronary artery disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Baseline levels of PAI 1 antigen and activity and tissue plasminogen activator ( t PA ) antigen were determined in fasting morning plasma samples . ^^^ On multiple regression analysis , the ACE genotypes did not appear to be significant predictors for plasma PAI 1 levels and t PA antigen after adjustment with age , sex , body mass index , plasma triglyceride , cholesterol , and glucose . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : We studied the coagulation cascade , natural clotting inhibitors , fibrinolytic proteins and resistance to activated protein C in 20 patients with advanced gastrointestinal cancer and 84 volunteers by measuring PT , APTT , fibrinogen , AT 3 , PC , PS , APC resistance , fibrinolytic system ( PLG , ANPL , PAI 1 , and t PA ) and activation peptides ( D Dimers , prothrombin 0 fragment 1 + 2 / F1 + 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Specifically , t PA antigen , plasminogen activator inhibitor 1 ( PAI 1 ) , and D dimer were measured preoperatively ( preop ) and on Postoperative Days ( POD ) 1 and 7 by the ELISA method . ^^^ While PAI 1 and t PA work in parallel , the marked elevation of PAI 1 on POD 1 ( although only slightly above reference values ) and continuing into POD 7 for subjects using compression devices requires further inquiry . ^^^ The elevation of PAI 1 in the face of elevated t PA and D dimer has been reported , but the comparison between patients using sequential compression devices and mini dose heparin has not been reported . ^^^ The reason for the elevation requires additional study into other influences on the synthesis , secretion , and / or function of PAI 1 that do not affect t PA . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MEASUREMENT AND RESULT : t PA , PAI 1 , t PA PAI 1 complexes , plasminogen , fibrinogen and plasmin alpha 2 antiplasmin complexes were measured serially by ELISA and free u PA by SDS PAGE with zymography . ^^^ The onset of symptoms was accompanied by a rise of t PA antigen followed rapidly by PAI 1 antigen . ^^^ CONCLUSION : Despite the sustained presence of active u PA in the circulation and of t PA antigen at the onset of symptoms , plasmin alpha 2 antiplasmin generation was largely suppressed by high levels of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Basal secretion of t PA and PAI 1 activity were higher in macrovascular endothelial cells . ^^^ Incubation of n LDL in concentrations ranging from 3 to 100 microM / ml LDL protein did not change t PA secretion , PAI 1 activity or procoagulant activity in both cell types . ^^^ Ox LDL ( 3 to 100 microM / ml LDL protein ) decreased t PA secretion in a concentration dependent manner from 30 . 9+ / 1 . 7 to 13 . 7+ / 30 ng / ml per 24 h per 10 ( 6 ) cells ( P < 0 . 01 ) , increased PAI 1 antigen from 2772+ / 587 to 4441+ / 766 ng / ml per 24 h per 10 ( 6 ) cells ( P < 0 . 05 ) as well as PAI 1 activity from 34+ / 6 to 55+ / 9 AU / ml per 24 h per 10 ( 6 ) cells ( P < 0 . 05 ) in macrovascular endothelial cells but had only minor effects on microvascular endothelial cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The results were as follows : In intensive infection with MSOF , PT and APTT increased significantly ; activity of F 2 , F 7 , F 10 and F 12 decreased significantly platelet count and Fn decreased markedly ; concentration of Fg and D Dimer increased significantly ; activity of PAI increased markedly ; activity of t PA and alpha ( 2 ) AP decreased slightly . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
So far , a variety of markers of a procoagulatory tendency e . g . elevated fibrinogen , coagulation factor 7 , factor 8 and von Willebrand factor , platelet hyperaggregation , increased plasma levels of D dimer , and decreased fibrinolytic capacity , e . g . characterized by increased levels of PAI 1 activity and decreased t PA concentrations have been identified prospectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
IL 1 ( 2 ng / mL ) specifically increased the accumulation of PAI 1 ( 4 . 4 + / 0 . 6 fold ; mean + / SD ; n = 9 ) without affecting tissue plasminogen activator ( t PA ) or urokinase plasminogen activator ( u PA ) levels , which remained unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Our results indicate that patients with more severe disease , as revealed by silent myocardial ischaemia , had lower levels of free t PA as a result of the excess of PAI 1 . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : The vascular endothelium , through the production of t PA and plasminogen activator inhibitor ( PAI 1 ) , is an important regulator of fibrinolysis . ^^^ PAI 1 and t PA antigen levels were measured by enzyme linked immunosorbent assay , and the net release across the forearm was calculated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Leukaemic and normal bone marrow samples were compared in terms of their content of the fibrinolytic agents , tissue plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and their inhibitors . plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of the specific activators ( u PA and t PA ) and the specific inhibitors ( PAI 1 and PAI 2 ) of the fibrinolytic system were analyzed in the peritoneal fluid in women suffering from intra abdominal adhesions , endometriosis or pelvic inflammatory diseases ( PID ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
D dimers , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , tumour necrosis factor alpha ( TNF alpha ) and full blood counts were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To investigate this question , we overexpressed plasminogen activator inhibitor 1 ( PAI 1 ) , an inhibitor of t PA and u PA , in a rat model of aortic aneurysm . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Abnormal expression of fibrinolytic genes [ e . g . , tissue type and urokinase type plasminogen activators ( t PA and u PA ) and their specific inhibitor ( PAI 1 ) ] and of the procoagulant molecule tissue factor ( TF ) , has been reported in various types of renal diseases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
By using suspended human umbilical vein endothelial cells ( HUVEC ) , which are free from the formation of extracellular matrix ( ECM ) where type 1 plasminogen activator inhibitor ( PAI 1 ) preferably accumulates and interacts with t PA with high affinity we demonstrated a relatively low affinity binding site for t PA on the cells and identified a novel t PAR . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In particular , we describe tissue factor , tissue factor pathway inhibitor , prothrombin fragment 1 . 2 , thrombin antithrombin complex , fibrinopeptide A , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , t PA PAI complex , Bbeta 15 42 related peptides , fibrinogen degradation products , fibrin degradation products , D dimer , platelet factor 4 , beta thromboglobulin , 5 hydroxytryptamine , thromboxane B 2 , prostacyclin , endothelin , angiotensin converting enzyme , soluble thrombomodulin , C 1 esterase inhibitor , anaphylotoxins C3a , C4a , and C5a , bradykinin , tumor necrosis factor , leukotriene C 4 , platelet activating factor , anti phospholipid antibody , and von Willebrand factor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : Substance P caused dose dependent increases in blood flow ( P < 0 . 001 ) and plasma t PA antigen ( P = 0 . 04 ) and activity ( P < 0 . 001 ) concentrations confined to the infused forearm , but had no effect on plasminogen activator inhibitor type 1 ( PAI 1 ) or von Willebrand factor concentrations . ^^^ L NMMA alone significantly reduced blood flow in the infused arm , but had no measurable effect on t PA or PAI 1 concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( T PA ) and plasminogen activator inhibitor ( PAI 1 ) levels in the conditioned medium of cultured porcine brain microvascular endothelial cells . ^^^ Vascular endothelial cells are known to produce tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ The supernatant obtained at respective time points was subjected to ELISA for t PA , PAI 1 and t PA PAI 1 complex . ^^^ It also proved that t PA and PAI 1 production by BMEC increases in a parallel fashion . ^^^ The present study suggests that t PA production by BMEC cultured under given stimulation increases , however , it is accompanied by concomitant PAI 1 production . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following haemostatic parameters were determined ; thrombelastography , fibrinogen , antithrombin 3 ( ATIII ) , thrombin antithrombin ( TAT ) complex , beta thromboglobulin ( beta TG ) , plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) , and plasminogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CONCLUSIONS : Our results indicate that factors in plasma from patients with decompensated cirrhosis directly stimulate t PA release from the vascular endothelial cells , while any increased PAI 1 release observed in comparable in vivo situations is probably an indirect response to an increase of t PA or a result of impaired hepatic clearance . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood was collected before and after each exercise test and was analyzed for the fibrinolytic variables , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activity , and lactate . ^^^ Maximal oxygen uptake , heart rate , and ventilation were greater ( P < 0 . 05 ) on the treadmill than during cycle ergometry , however , blood lactate was similar between modes . t PA activity significantly increased with exercise ( P < 0 . 05 ) and there was a trend toward a reduction in PAI 1 activity with exercise , but this did not reach statistical significance . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Thrombin can regulate the fibrinolytic system by increasing the endothelial production of both tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ The purpose of the present study was to examine the roles of Galpha 1 protein and tyrosine kinases in the thrombin signal transduction of t PA and PAI 1 production from human adult vein endothelial cells ( HAVEC ) . t PA and PAI 1 antigen were analysed in conditioned medium from cultured HAVEC after 16 h incubation . ^^^ Thrombin increased t PA and PAI 1 production ( 234 + / 42 % and 211 + / 42 % , respectively ) . ^^^ Pertussis toxin ( PTX ) ( inhibiting Galpha 1 pathway ) reduced basal PAI 1 ( 66 + / 8 % ) , but had only a weak influence on basal t PA production . ^^^ Pertussis toxin and genistein ( inhibiting tyrosine kinase ) significantly reduced the thrombin induction of both t PA and PAI 1 ( PTX : 142 + / 23 % and 146 + / 19 % , respectively , genistein : 156 + / 42 % and 76 + / 24 % , respectively ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 ovalbumin , PAI 1 antithrombin 3 and PAI 1 P 13 ( Val > Glu ) revealed specific activities of 86+ / 15 % , 77+ / 11 % , and 100+ / 30 % respectively , towards t PA and similar inhibitory properties towards u PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , plasma high density lipoprotein cholesterol ( HDL C ) , HDL 2 C , HDL C / TC , insulin , fibrinogen , fibrin D dimer , PAI antigen , tissue plasminogen activator ( t PA ) activity , and t PA antigen levels all were less favorable ( P < . 05 ) in swimmers versus runners . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endothelial cells express fibrinolytic proteins including : urokinase ( u PA ) and tissue type ( t PA ) plasminogen activators , type 1 ( PAI 1 ) and 2 ( PAI 2 ) plasminogen activator inhibitors , and u PA receptor ( u PAR ) . ^^^ Antigen levels for t PA , PAI 1 , PAI 2 , and u PAR were reduced greatly in apoptosis ( p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Elevated plasminogen activator inhibitor 1 ( PAI 1 ) is a risk factor for thrombosis , and inhibitors of the interaction between PAI 1 and tissue plasminogen activator ( t PA ) have antithrombotic and prothrombolytic activity in animals . ^^^ The activity of MA33H1 against rat PAI 1 was confirmed using two chain t PA and a chromogenic substrate . ^^^ Thus , a monoclonal antibody which transforms PAI 1 to a t PA substrate prevents thrombus formation in the rat with no effect on bleeding time at a higher dose . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Antithrombin 3 , thrombin antithrombin 3 complexes , heparin cofactor 2 , protein C , protein S , tissue plasminogen activator , ( t PA ) D dimer and plasminogen activator inhibitor ( PAI 1 and PAI 2 ) activities in normal and gestational diabetes pregnancies were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Moreover the oxidized LDL and AGE up regulate the expression of tissue factor , and t PA inhibitor , PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fasting samples were analysed for serum lipids ( total , HDL and LDL cholesterol and triglyceride ) and plasma fibrinogen , factor 7 coagulant ( FVII : C ) activity , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The levels of t PA activity , t PA antigen and t PA inhibitor 1 ( PAI 1 ) antigen were estimated in the supernatants of cultured human fetal mesangial cells incubated for 72 h with thrombin , IL Ibeta , IL 6 , IL 10 , and transforming growth factor beta ( TGF beta ) . ^^^ The t PA activity was measured by an amidolytic assay , and the levels of t PA antigen and PAI 1 antigen were also measured by enzyme linked immunosorbent assay . ^^^ Thrombin increased t PA activity and TGF beta decreased it in parallel with t PA antigen level , although these agents did not affect the synthesis of PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To evaluate the effect of the uremic environment on the fibrinolytic activity of endothelial cells , we studied plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) production by human umbilical vein endothelial cells ( HUVEC ) in culture , exposed either to uremic or normal sera , before and after cytokine stimulation . ^^^ Before cytokine stimulation , no difference in the HUVEC supernatant concentration of t PA and PAI 1 was found among the groups studied . ^^^ After stimulation with interleukin ( IL ) 1 and tumor necrosis factor ( TNF ) alpha , the HUVEC supernatant levels of PAI 1 in the uremics were higher than in the controls , whereas the supernatant levels of t PA did not differ . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It has been proved by many authors that fibrinolytic activity is depressed in patients with vascular thrombosis , due to increased levels of fibrinolytic inhibitors mainly plasminogen activator inhibitor ( PAI 1 ) , and / or decreased levels of tissue plasmogen activator ( t PA ) . ^^^ Low levels of t PA and increased levels of PAI 1 have been found in states of special risk of thrombosis : obesity , diabetes mellitus , postoperative states , rheumatoid arthritis , malignancies and other diseases such as Behcet ' s syndrome . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Subsequent fibrin autography patterns indicated the presence of u PA , PAI 1 , and t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of plasminogen activator inhibitor 1 ( PAI 1 ) activity and tissue PA ( t PA ) antigen were measured in Type 2 diabetes subjects with ( n=40 ) and without ( r=80 ) acute stroke compared to non diabetic subjects with ( n=80 ) and without ( n=80 ) acute ischaemic stroke . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
During preeclampsia t PA and PAI 1 levels are markedly increased in plasma , and in cases of intrauterine growth retardation , u PA and PAI 2 levels are decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Both AIIt and the p 11 subunit protected t PA and plasmin from inactivation by PAI 1 and alpha 2 antiplasmin , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These results underline the involvement of serine proteases and extracellular matrix components such as the PAI 1 / t PA axis in the excitotoxic cascade . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In both groups the activated partial thromboplastin time ( APTT ) , prothrombin ratio ( INR ) , fibrinogen , euglobulin lysis time ( ELT ) , platelet number and platelet adhesion and aggregation , tissue plasminogen activator antigen ( t PA Ag ) and activity of plasminogen activator inhibitor ( PAI 1 ) were tested and compared . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endothelial cells have four key functions that alter in the process of inflammation : 1 a ) Regulation and control of leukocyte traffic through the expression of adhesion molecules ( selectins E and P , molecules of immunoglobulin superfamily ICAM 1 , ICAM 2 , VCAM ) ; 1 b ) They are also able to amplify leukocyte activation through the production of proinflammatory cytokines like IL 1 , IL 6 and chemokines like IL 8 and RANTES molecules ; 2 ) Regulation of vascular tone by production of PGI 2 , EDRF / NO and elements of local renin angiotensin system ; 3 ) Regulation of local coagulation by controlling the production of t PA and PAI 1 ; 4 ) Regulation of the vascular permeability . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of smoking on SOD , MDA , t PA , PAI 1 were studied in 20 healthy men ( 10 smokers and 10 nonsmokers ) . ^^^ After acute smoking , SOD decreased and MDA increased significantly in all the subjects , t PA and PAI 1 activities were increased immediately after acute smoking . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Venous occlusion for euglobulin lysis time , t PA antigen and activity , and PAI 1 antigen and activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Euglobulin clot lysis time ( ECLT ) , tissue plasminogen activator ( t PA ) activity , and plasminogen activator inhibitor ( PAI ) activity were measured before and after VOT in a group of 26 consecutive patients presenting with retinal vein occlusion and in 15 healthy age and sex matched controls . ^^^ Patients had non significantly lower t PA and higher PAI activities compared with controls . ^^^ Patients had significantly lower t PA activity ( p = 0 . 0232 ) and significantly higher PAI activity ( p = 0 . 0292 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate von Willebrand factor ( vWF ) , tissue plasminogen activator antigen ( t PA ) and its activity before and after venous occlusion , plasminogen activator inhibitor ( PAI 1 ) , dehydroepian drosterone sulphate ( DHEAS ) , and established clinical risk factors as long term predictors for reinfarction and mortality . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasma factors studied were the following : 1 ) plasma fibronectin and thrombomodulin as markers of endothelial cell damage ; 2 ) beta thromboglobulin as platelet activity markers ; 3 ) 8 : C factor and fibrinopeptide A as coagulation activity markers ; 4 ) coagulation inhibitors such as protein C and protein S activity ; 5 ) tissue plasminogen activator ( t PA ) , plasminogen level and plasminogen activator inhibitors ( PAI ) as fibrinolytic activity markers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were processed for the determination of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Our results thus show that short term treatment with the ACE inhibitor captopril , at least in healthy subjects on an unrestricted NaCl intake , does not affect the fibrinolytic balance between t PA and PAI 1 or the von Willebrand factor . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) , another two endothelium derived proteins and modulators of blood fibrinolytic activity , have not been reported in hyperthyroidism . ^^^ METHODS : The release of t PA by vascular endothelial cells after venous occlusion , basal blood concentrations of t PA , PAI 1 and vWF were studied in 33 patients with hyperthyroidism , with most of them followed up 30 days after iodine radiotherapy . ^^^ RESULTS : Compared with control subjects , both the basal t PA levels in plasma and the increase in t PA levels with venous occlusion were significantly decreased in patients ( P < 0 . 001 ) , whereas plasma PAI 1 and vWF levels were increased greatly ( P < 0 . 001 ) . ^^^ At follow up , patients with normal plasma free thyroxine ( T 4 ) and total tri iodothyronine ( T 3 ) showed similar plasma levels of t PA , PAI 1 , vWF and release of t PA from endothelial cells as the control subjects ( P > 0 . 05 ) . ^^^ In those with persistent elevated T 4 or T 3 levels , however , the basal plasma t PA level and the released t PA from endothelial cells during venous occlusion were significantly lower than in the control subjects ( P < 0 . 001 and P < 0 . 005 ) , and blood concentrations of PAI 1 and vWF did not return to the normal range ( P < 0 . 001 and P < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Human mesothelial cells ( HMCs ) have an important role in maintaining an adequately functioning fibrinolytic system in the peritoneal cavity by secreting the fibrinolytic enzymes tissue type and urokinase type plasminogen activator ( t PA and u PA ) , as well as a specific PA inhibitor , PA inhibitor type 1 ( PAI 1 ) . ^^^ Tumor necrosis factor alpha ( TNFalpha ) diminished the fibrinolytic activity of HMCs by decreasing t PA and increasing PAI 1 synthesis . ^^^ Interestingly , chloroquine also quenched the TNFalpha induced changes in t PA and PAI 1 mRNA levels . ^^^ CONCLUSIONS : Receptor mediated endocytosis plays a crucial role in regulating the fibrinolytic capacity of HMCs by its participation in the degradation of t PA and u PA , and in the TNFalpha induced decrease in t PA and the increase in PAI 1 expression . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
TNK is a mutant of tissue plasminogen activator ( t PA ) with high fibrin specificity , resistance to plasminogen activator inhibitor 1 and slow plasma clearance , which is administered in a single intravenous bolus . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We , therefore , studied the associations between fasting serum oestradiol , plasma cholesterol and triglyceride , and Factor 7 activity , PAI activity , t PA antigen , fibrin D dimer , and vWF antigen in 10 women , each sampled on 6 occasions between 10 weeks and 35 weeks during normal pregnancy . ^^^ Strong and similar individual correlations were observed between increases in FVII , PAI , t PA and D dimer ( but not vWF ) and increases in both oestradiol and triglyceride . ^^^ We , therefore , suggest that oestradiol induced hypertriglyceridaemia may be a cause of elevations in plasma Factor 7 activity , PAI and t PA , and fibrin turnover ( D dimer ) during normal pregnancy , but is poorly related to the increase in vWF antigen . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NaPPS and CaPPS stimulated the release of tissue plasminogen activator ( t PA ) , superoxide dismutase , and lipases from vascular endothelium while concomitantly decreasing plasma levels of the endogenous plasminogen activator inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activity of both plasminogen tissue activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) were also determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Although treatment with ethanol alone did not affect t PA gene transcription or mRNA expression , it augmented isoproterenol ( ISO ) stimulated t PA gene transcription and mRNA levels by 3 . 4 and 2 . 8 fold , respectively , and decreased plasminogen activator inhibitor 1 mRNA levels by 65 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , we propose a role for HGF / SF in wound repair in the skin : HGF / SF produced by activated fibroblasts increases in keratinocytes the expression of PAI 1 , which leads to increased matrix stability during the repair process and which could also limit activation of HGF / SF by proteases such as urokinase type PA ( u PA ) or tissue type PA ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Forearm blood flow was assessed by venous plethysmography ; t PA and plasminogen activator inhibitor 1 ( PAI 1 ) antigen values were expressed as flow dependent ( net release , the product of venoarterial concentration gradient and forearm blood flow ) or independent ( absolute and fractional concentration gradients ) indices . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following coagulation tests were performed before and after near maximum exercise : prothrombin time ( PT ) , partial activated thromboplastin time ( PTT ) , fibrinogen ( FBG ) , antithrombin 3 ( ATIII ) , protein C ( PC ) , protein S ( PS ) , prothrombin fragment 1 + 2 ( F 1 + 2 ) , tissue activator of plasminogen ( t PA ) and its inhibitor ( PAI ) . ^^^ RESULTS : The most significant results showed a low basal PC in the rowers which decreased further after near maximum exercise ; significantly higher basal activities of ATIII , PC and PS in the marathon runners compared to the rowers ; a high proportion of weightlifters showed a reduction in t PA after exercise and an increase of PAI ; the controls were the only group in which fibrinolytic activity and all the circulating anticoagulants increased after near maximum exercise . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This treatment reduced the consumption of platelets and protein C and attenuated the increase of PAI 1 more efficiently than r hirudin or t PA alone . r Hirudin plus t PA also resulted in the lowest formation of fibrin deposits in the kidneys . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAOD patients had significantly higher plasma levels of t PA antigen ( 12 . 0+ / 4 . 9 vs . 9 . 2+ / 5 . 5 ng / ml ) , PAI 1 antigen ( 34 . 8+ / 22 . 1 vs . 27 . 2+ / 23 . 6 ng / ml ) , PAI 1 activity ( 10 . 0+ / 6 . 5 vs . 8 . 0+ / 8 . 0 U / ml ) , PCI ( 188 . 2+ / 55 . 6 vs . 134 . 1+ / 75 . 5 % as compared with normal human plasma ) , and fibrinogen ( 420 . 2+ / 92 . 6 vs . 261 . 9+ / 32 . 7 mg / dl ) as compared with controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
W . of the lower limbs , ankle / brachial index , dosage of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , hematocrit , hematic filtration , and viscosity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this study the effect of a high dose of dexamethasone on plasminogen activator activity ( PAA ) , t PA antigen level , plasminogen activator inhibition ( PAI ) and plasmin inhibition ( Pl ) in rat heart , brain , liver , lungs and kidneys was investigated in both sexes . ^^^ CONCLUSION : These changes of PAA , PAI and t PA antigen level in heart , brain and liver induced by dexamethasone might be of importance regarding the involvement of glucocorticoids and plasminogen activators / plasmin in many pathophysiological conditions . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A group of 58 patients who had survived to a first infarction episode were studied ; the cardiovascular risk factors were determined and blood concentrations of fibrinogen , t PA , PAI and FRW measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Endothelial cell fibrinolysis : transcriptional regulation of fibrinolytic protein gene expression ( t PA , u PA , and PAI 1 ) by low alcohol . ^^^ Endothelial cells ( ECs ) synthesize fibrinolytic proteins , t PA , u PA , and PAs inhibitor , PAI 1 . ^^^ Further nuclear transcription run on assays and transient transfection experiments , using pPAs / luc and pPAI 1 / luc promoter constructs , demonstrated that low ethanol transcriptionally upregulates t PA and u PA gene expression and downregulates PAI 1 gene expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The effects of endothelin 1 ( ET 1 ) on the production of plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) by human brain derived endothelial cells in culture were studied . ^^^ At 100 nmol / L , ET 1 increased PAI 1 production by 88+ / 6 % within 72 hours , and increased PAI 1 mRNA expression within 1 hour of stimulation ; there was no significant effect on t PA production . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To determine the ability of radiation to modulate mesangial cell expression of various molecules involved in promoting extracellular matrix ( ECM ) accumulation [ fibronectin , plasminogen activator inhibitor 1 ( Pai 1 ) , and tissue inhibitor of metalloproteinase 2 ( Timp 2 ) ] and degradation ( Tgfb , plasminogen activators u PA or t PA , matrix metalloproteinases Mmp 2 and Mmp 9 ) , primary cultures of rat mesangial cells ( passage number 6 11 ) were placed in serum free medium 24 h prior to irradiation with single doses of 0 . 5 20 Gy ( 137 ) Cs gamma rays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic parameters : euglobulin clot lysis time , t PA activity ( amidolytic assay ) and antigen ( ELISA ) and t PA inhibitor ( PAI ) activity ( amidolytic assay ) were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the citrate plasma of patients studied , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , plasmin alpha ( 2 ) antiplasmin ( PAP ) complexes , fibrin fibrinogen degradation products ( FDP ) and euglobulin lysis time ( ELT ) were measured . ^^^ In the group studied intraoperative values of t PA Ag , PAP and FDP were significantly higher , PAI 1 Ag concentration decreased and ELT was reduced . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It would be suggested that regular aerobic training may decrease in fibrinogen , platelet aggregation and PAI 1 antigen , and increase in t PA activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Recombinant adenoviral vectors expressing u PA , t PA , PAI 1 and PAI 2 were employed to correlate the expression of components of the fibrinolytic system with the invasiveness of HT 1080 tumor cells . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes in t PA , PAI 1 gene expression of coronary arteries with preconditioning in acute myocardial infarction rat ] . ^^^ The changes of t PA , PAI 1 gene expression and t PA activity were investigated in coronary arteries of Acute Myocardial Infarction ( AMI ) rat by nucleic acid molecular hybridization and spectrophotometric assay . ^^^ The effect of preconditioning on t PA and PAI 1 was observed in this model . ^^^ The results indicated that : ( 1 ) Tissue t PA activity in ischemia was significantly decreased than that in control ( P < 0 . 01 ) . t PA activity was obviously increased in preconditioning group ( P < 0 . 01 compared with ischemia group ) . ( 2 ) t PA and PAI 1 mRNA expression in ischemia were higher than that in control , especially PAI 1 4 . 6 times higher than that in control ) , while PAI 1 expression lower in preconditioning rat . ( 3 ) In situ hybridization showed t PA and PAI 1 were primarily expressed by endothelial cells in vessels of rat heart . ^^^ These data suggested that abnormal expression of PAI 1 and t PA was involved in AMI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CONCLUSIONS : This study showed that the PAI 1 level increase is more pronounced after SK treatment than after t PA treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following parameters were determined : plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , D dimer , beta thromboglobulin ( beta TG ) , thrombin antithrombin ( TAT ) complex , fibrinogen , Factor 7 , platelets , haematocrit and haemoglobin levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood levels of lipoproteins , glucose , iron and transferrin , fibrinogen , tissue plasminogen activator ( t PA ) antigen level and activity and plasminogen activator inhibitor ( PAI 1 ) antigen level and activity were also determined . ^^^ Other risk factors displaying statistically significant correlations to mean carotid IMT were t PA antigen level and activity , PAI 1 antigen level and level of high density lipoprotein cholesterol . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It is known that angiotensin 2 ( Ang 2 ) exerts an antifibrinolytic effect by stimulating synthesis of plasminogen activator inhibitor type 1 ( PAI 1 ) , a specific inhibitor of tissue plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have tried to answer this question by measuring DNA polymorphisms of selected cardiovascular risk indicators [ factor 7 , FVII ( R / Q353 , intron 7 ( 37bp ) n , and 323ins10 ) , beta fibrinogen ( 455G / A ) , plasminogen activator inhibitor type 1 , PAI 1 ( 675 ( 4G / 5G ) ) , tissue plasminogen activator , t PA ( intron 8 ins 311 ) , platelet receptor glycoprotein IIb / IIIa , GPIIb / IIIa ( L / P33 ) , prothrombin ( 20210G / A ) , methylene tetrahydrofolate reductase , MTHFR ( A / V114 ) , angiotensin converting enzyme , ACE ( intron 16 ins 287 ) , and angiotensinogen ( M / T235 ) ] . ^^^ The frequencies of the high risk alleles in centenarians were : for FVII R / Q353 0 . 91 ; for FVII intron 7 ( 37bp ) n 0 . 67 ; for FVII 323 ins 10 0 . 90 ; for fibrinogen 0 . 16 ; for PAI 1 0 . 52 ; for t PA 0 . 59 ; for GPIIb / IIIa 0 . 16 ; for prothrombin 0 . 008 ; for MTHFR 0 . 33 ; for ACE 0 . 52 ; and for angiotensinogen 0 . 36 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Mean levels of PAI activity and t PA antigen were significantly lower in OC users than in non users . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present morphological study was therefore designed to investigate the occurrence and distribution of the tissue and urokinase plasminogen activators ( t PA and u PA ) , the u PA receptor ( u PAR ) and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) in normal human testis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI ) activity , t PA activity , and prothrombin fragment ( PF ) 1+2 were measured before induction of anesthesia , immediately before aortic clamp release , and 5 minutes and 24 hours after aortic clamp release . ^^^ The differences in PAI activity , t PA activity , and PF 1+2 persisted throughout the course of surgery , but there was no significant difference between the groups at 24 hours . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In all patients levels of total cholesterol , triglyceride , HDL cholesterol , LDL cholesterol , lipoprotein ( a ) ( Lpa ) C reactive protein ( CRP ) , fibrinogen , thrombin / antithrombin 3 complex ( TAT ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and von Willebrand antigen were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For the patients with ' medium baseline values ' , total plasminogen activator inhibitor 1 ( PAI 1 ) and t PA PAI 1 complex levels decreased , while the changes in lipids and lipoproteins were not significant after amlodipine treatment . ^^^ For the patients with ' high baseline values ' , the mean triglyceride and very low density lipoprotein cholesterol ( VLDLC ) levels were reduced while the reductions in total and free PAI 1 and the increase in tissue plasminogen ( t PA ) levels were not significant after amlodipine treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We have examined the serum levels of the plasminogen activator inhibitor type 1 ( PAI 1 ) antigen and activity , tissue plasminogen activator ( t PA ) antigen and activity , soluble thrombomodulin ( sTM ) , and tissue factor pathway inhibitor ( TFPI ) before and after reaching the target blood pressure ( < 140 / 90 mm Hg ) in 13 hypertensive patients receiving perindopril ( mean age 40+ / 11 years , 6 women , 7 men ) and in 12 patients receiving losartan ( mean age 38+ / 9 years , 6 women , 6 men ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Whereas wild type PAI 1 ( wtPAI 1 ) exhibits inhibitory properties towards t PA and u PA to an extent of 60 80 % of the theoretical maximum , PAI 1 delhF did not exert any detectable inhibitory properties , but behaved as a stable substrate . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolytic factors such as tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and t PA PAI 1 complex ( PAI C ) of specimens were measured by enzyme linked immunoassay . ^^^ In the control group , concentrations of t PA as well as PAI 1 in amniotic fluid remained at similar levels from 15 to 18 weeks , although PAI 1 levels were more than 10 times higher compared with t PA levels . ^^^ Levels of t PA and PAI 1 in the severe preeclamptic group were not different from those of the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The remaining tissue plasminogen activator ( t PA ) of the sample consisting of beta 2 GPI , two chain recombinant t PA , and plasminogen activator inhibitor ( PAI ) 1 was measured by a chromogenic assay using the synthetic substrate S 2251 , Glu plasminogen , and soluble fibrin monomer . beta 2 GPI protected t PA activity from inhibition by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied 428 patients ( 44 % men ) and measured blood levels of fibrinogen , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator antigen ( t PA ) , plasma viscosity , von Willebrand factor , D dimer and platelet count , both at baseline and after 90 days of daily immersion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Cholesterol and triacylglycerol ( TG ) correlated positively with t PA antigen ( t PAag ) ( r = 0 . 36 and r = 0 . 38 ) , PAI 1 antigen ( PAI 1ag ) ( r = 0 . 35 and r = 0 . 50 ) , P selectin ( r = 0 . 26 and r = 0 . 27 ) ( all P < 0 . 0001 ) and WBC ( r = 0 . 17 , P < 0 . 007 and r = 0 . 18 , P < 0 . 004 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : To measure the tissue plasminogen activator antigen ( t PA Ag ) content and plasminogen activator inhibitor type 1 ( PAI 1 ) activity of SRF and blood . ^^^ T PA Ag concentration and PAI 1 activity in subretinal fluid and citrated plasma and their relation to patients ' age and sex , the duration and extent of retinal detachment , and degenerative changes of the retina were assessed . ^^^ RESULTS : The median t PA Ag concentration in 22 samples of SRF was 6 . 7 ng / ml ( interquartile range 3 . 6 ng / ml ) and PAI 1 activity 14 . 0 IU / ml ( interquartile range 7 . 5 IU / ml ) . ^^^ The median levels of t PA Ag and PAI in plasma were respectively 10 . 7 ng / ml ( interquartile range 8 . 6 ng / ml ) and 15 . 7 IU / ml ( interquartile range 12 . 2 IU / ml ) . ^^^ There were no differences between the t PA Ag concentration and PAI 1 activity in SRF and blood . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DEVELOPMENT : The conditions related to increased risk of thrombosis are : the congenital thrombophilias due to deficiency of protein C , protein S or antithrombin 3 , resistance to protein C activated by Leiden ' s factor 5 cofactor of protein C with genetic mutation ; the primary antiphospholipid syndrome with anticardiolipin antibodies and lupus inhibitor ; platelet disorders , deficit of heparin cofactor 2 , deficit of plasminogen and plasminogen tissue activator ( t PA ) and increase in the inhibitor of plasminogen tissue activator ( PAI 1 ) ; alterations in factors of coagulation such as deficits of factor 7 and factor 13 , mutation of prothrombin 20210 > A , increase in factor 8 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
These patients had elevated PAI activity and antigen and t PA antigen levels at rest and after venous occlusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activators u PA ( urokinase ) and t PA ( tissue ) as well as the inhibitors PAI 1 and PAI 2 are present in gingival crevicular fluid in concentrations significantly greater than in plasma . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Parameters monitored included blood loss , prothrombin F1 . 2 , thrombin antithrombin complexes , t PA , PAI 1 , plasminogen , fibrin D dimer , and plasma factor 13 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We studied tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) levels in plasma and peritoneal effluent in 10 children on continuous ambulatory peritoneal dialysis ( CAPD ) before , and 8 and 12 weeks after , treatment with recombinant human erythropoietin ( rHuEPO ) . ^^^ Plasma t PA and PAI 1 levels were lower in patients than in controls during the study . ^^^ No correlation was observed between levels of t PA and PAI 1 in plasma and in peritoneal effluent . ^^^ These results suggest that plasma t PA levels can be increased by rHuEPO treatment , while plasma PAI 1 levels are associated with hemoglobin levels . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes of serum TNF alpha level , t PA activivty and PAI activity in patients with silent myocardial ischemia or silent cerebral ischemia ] . ^^^ Tumor necrosis factor alpha ( TNF alpha ) level , tissue typed plasminogen activator ( t PA ) activity and PA inhibitor ( PAI ) activity were determined in three groups : ( 1 ) 25 NIDDM patients with silent myocardial ischemia ( SMI ) or silent cerebral ischemia ( SCI ) ; ( 2 ) 18NIDDM patients without SMI or SCI ; ( 3 ) 20 age matched normal controls . ^^^ The result showed that the TNF alpha level and PAI activity in the ischemia group were the highest and the t PA activity in the ischemia group was the lowest , as compared with those in the other two groups respectively . ^^^ It suggests that in NIDDM patients who have high TNF alpha , high PAI activity , low t PA , and even no symptoms and signs of MI or CI , anticoagulant therapy might be useful to prevent the progression of diabetic macroangiopathies . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To better understand the regulation of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) during liver transplantation , we used a computer model of the human circulatory system to simultaneously evaluate the effect of t PA secretion , t PA inhibition by PAI 1 , hepatic clearance of t PA , blood loss , transfusion and hemodynamics on t PA and PAI 1 levels during liver transplantation in three patients that differed in severity of liver disease , blood loss and anhepatic changes in t PA . ^^^ During the anhepatic stage , when hepatic t PA clearance was eliminated : ( 1 ) the expected rise in t PA was modulated by the extent of bleeding , which acted as an alternate t PA clearance mechanism ; and ( 2 ) the ratio of t PA : PAI 1 was increased due both to lower t PA clearance and reduced PAI 1 secretion . ^^^ Recirculation of the new liver was associated with renewed clearance of t PA , an acute phase increase in PAI 1 and a drop in the t PA : PAI 1 ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The majority of studies focussed on markers of thrombin activation , fibrinogen , fibrin degradation products and t PA and its main inhibitor PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
FGF 2 isoforms of 18 and 22 . 5 kDa differentially modulate t PA and PAI 1 expressions on the pancreatic carcinoma cells AR 4 2J : consequences on cell spreading and invasion . ^^^ The 22 . 5 kDa FGF 2 reduced t PA and PAI 1 synthesis 2 fold . ^^^ Addition of recombinant 18 kDa FGF 2 ( rFGF 2 ) to cell cultures resulted in increased t PA and decreased PAI 1 expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study was performed to analyze the relationship between portal hypertension and alterations of the endothelium derived proteins thrombomodulin , plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) , which were determined in plasma samples of 28 alcoholic cirrhotic patients and 46 controls . ^^^ Although cirrhotics showed lower levels of PAI 1 , but higher thrombomodulin and t PA levels than controls , no relationship was observed between thrombomodulin , t PA or PAI 1 and portal pressure . ^^^ Therefore , the hypothesis that splachnic endothelial damage secondary to portal hypertension leads to altered thrombomodulin , t PA and PAI 1 levels in alcoholic cirrhosis is not supported by the results of this study . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in cell conditioned medium were determined by ELISA and the level of PAI 1 mRNA was determined using northern hybridisation . ^^^ RESULTS : Under basal conditions ( 5 mM glucose ) , HREC produced PAI 1 , t PA , and trace amounts of u PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and PAI 1 of choledochal bile were measured by enzyme linked immunosorbent assay in patients with cholangitis due to acalculous bile duct obstructions ( n = 9 ) , choledocholithiasis with cholangitis ( n = 20 ) , and normal bile ( n = 7 ) . ^^^ Human choledochal bile possesses t PA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : To determine the ability of radiation to modulate kidney tubule epithelial cell expression of various molecules involved in regulating extracellular matrix accumulation ( collagen types 1 and 3 , fibronectin , plasminogen activator inhibitor 1 ( PAI 1 ) , TGF beta and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ) and degradation ( plasminogen activators u PA or t PA , MMP 2 and MMP 9 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of fibrinogen , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were measured on admission and discharge . ^^^ PAI 1 and t PA were measured by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The preliminary results of other studies on the effects of 4 HPR on tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and urokinase plasminogen activator ( uk PA ) and on the relevance of circulating p 53 antibodies with relapse will be also presented . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activity of plasminogen activator inhibitor ( PAI 1 ) , platelet adhesion and aggregation , antigens of tissue and urokinase plasminogen activators ( respectively , t PA Ag and u PA Ag ) , euglobulin lysis time ( ELT ) , complexes of plasmin antiplasmin ( PAP ) and fibrin degradation products ( FDP ) were tested before and after fourteen days administration of 20 30 mg / d prednisone . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In HTG , basal fibrinolytic activity , ie tissue plasminogen activator ( t PA ) activity , was impaired as a result of high plasminogen activator inhibitor type 1 ( PAI 1 ) antigen and activity . ^^^ The observation that both insulin and triglycerides correlate with PAI 1 , whereas triglycerides were involved only in the increased secretion of t PA , suggests that these two proteins are regulated by different mechanisms . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The remaining tissue plasminogen activator ( t PA ) of the sample consisting of beta 2 GPI , two chain recombinant t PA , plasminogen activator inhibitor ( PAI ) 1 was measured by a chromogenic assay using synthetic substrate S 2251 , Glu plasminogen , and soluble fibrin monomer . ^^^ Without PAI 1 , beta 2 GPI did not affect t PA activity . ^^^ When 14 . 3 ng / ml PAI 1 was added to 3 . 6 U / ml t PA , the remaining t PA activity was increased from 48 . 9 % to 60 . 4 % by the addition of beta 2 GPI ( 190 microg / ml ) . ^^^ The beta 2 GPI seems to protect t PA activity from the inhibition by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Monoclonal aCL in the presence of beta 2GPI also increased the activity of plasminogen activator inhibitor ( PAI ) 1 in the mixture of tissue plasminogen activator ( t PA ) and PAI 1 by inhibiting the function of beta 2GPI , which increased the remaining t PA activity in the mixture . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
It was found that cadmium promotes the synthesis of plasminogen activator inhibitor 1 ( PAI 1 ) whereas lead inhibits the synthesis of tissue plasminogen activator ( t PA ) in vascular endothelial cells ; consequently , both heavy metals reduced fibrinolytic activity in the liquid phase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The obese subjects presented significantly elevated values for insulin ( P < . 001 ) , tissue plasminogen activator ( [ t PA ] P < . 001 ) , plasminogen activator inhibitor 1 ( [ PAI 1 ] P < . 001 ) , and fibrinogen ( P < . 001 ) with respect to the control group . ^^^ On the other hand , t PA was correlated with insulin and PAI 1 but not with the BMI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin lysis time ( ELT ) , tissue plasminogen activator activity ( t PA : act ) and antigen ( t PA : Ag ) , PAI 1 activity ( PAI 1 : act ) , and antigen ( PAI 1 : Ag ) were assayed on blood samples drawn before and 20 min after the venous occlusion test ( VO ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood t PA antigen differed as well , yet basal endogenous fibrinolytic activity was decreased because of the high PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , thrombi in wt mice , t PA ( / ) and u PA ( / ) mice were comparable , substantiating efficient inhibition of fibrinolysis by the combined PAI 1 / alpha ( 2 ) AP action . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition to analyses of t PA binding to plasminogen activator inhibitor 1 ( PAI 1 ) in the extracellular matrix ( ECM ) and to the t PAR , we further evaluated the binding of three t PA mutants , deltaFE1X t PA lacking finger ( F ) , epidermal growth factor like ( E ) domains and one sugar chain at Asn 177 thus comprising two kringles ( K 1 and K 2 ) and protease ( P ) domains , deltaFE3X t PA with three glycosylation sites deleted at Asn 117 , 184 , and 448 , and deltaFEK 1 t PA comprising K 2 and P domains without glycosylation . ^^^ Wild type t PA bound to ECM with high affinity , which was completely blocked by anti PAI 1 IgG . ^^^ These results suggested that the high affinity binding of t PA mainly occurred with PAI 1 on ECM while the low affinity binding was with t PAR . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinopeptide A ( FP A ) , D Dimer , plasminogen activators ( PA ) of the urokinase ( u PA ) or tissue type ( t PA ) , PA inhibitor 1 ( PAI 1 ) and alpha 2 antiplasmin ( alpha 2 AP ) were determined by ELISA technique . ^^^ U PA , but not t PA levels were significantly reduced in all ILD groups . alpha 2 AP was markedly elevated throughout , whereas PAI 1 levels were lowered . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator antigen ( t PA Ag ) and tissue plasminogen activator inhibitor ( PAI 1 ) in the course of hemodialysis in patients with chronic renal failure ] . ^^^ In 10 patients with chronic renal insufficiency we are tissue plasminogen activator antigen ( t PA Ag ) , tissue plasminogen activator inhibitor ( PAI 1 ) and euglobulin lysis time ( ELT ) designed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen and activity were measured at baseline and 6 hours after the dose . ^^^ Acute administration of losartan but not of enalapril reduced plasma t PA ( 11 % ; P=0 . 003 ) and PAI 1 ( 38 % ; P < 0 . 001 ) antigen concentrations , which was associated with increases in t PA ( 29 % ; P=0 . 03 ) and decreases in PAI 1 ( 48 % ; P=0 . 01 ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of thrombin antithrombin ( TAT ) complexes , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) activities were measured using commercially available kits . ^^^ Plasma t PA activity levels peaked at 2 h ( nine fold increase ) , whereas the peak of PAI 1 activity was delayed ( eight fold increase at 4 h ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The role of the t PA I / D and PAI 1 4G / 5G polymorphisms in African American adults with a diagnosis of myocardial infarction or venous thromboembolism . ^^^ To determine whether or not the PAI 1 4G / 5G and t PA I / D polymorphisms in African Americans were linked to cardiovascular disease , the association of these polymorphisms to disease expression was analyzed in a recently completed case control study of myocardial infarction or venous thromboembolism among African Americans . ^^^ It was also found that genotype frequencies for both PAI 1 4G / 5G and t PA I / D polymorphisms in African American adults were different from those reported for both U . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Epidemiological studies have shown that the haemostatic parameters Fibrinogen ( Fg ) , Factor 7 ( F 7 ) , Factor 8 ( F 8 ) , von Willebrand factor ( vWF ) , Tissue Plasminogen Activator ( t PA ) , Plasminogen Activator Inhibitors ( PAI ) are risk factors / markers of ischemic cardiovascular disease . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of plasma PAI 1 , tissue plasminogen activator ( t PA ) , and t PA 10 PAI 1 complex were similar in the CAD ( ) and CAD ( + ) groups , but were greater on day 1 in the AMI group compared with the 2 CAD groups . ^^^ The serial changes of the plasma t PA and t PA PAI 1 complex during the course of AMI were similar to those of the plasma PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue factor ( TF ) , thrombin antithrombin 3 complex , plasminogen activator inhibitor ( PAI ) 1 , tissue plasminogen activator ( t PA ) , beta thromboglobulin , and platelet factor 4 were measured by enzyme linked immunosorbent assay . ^^^ PAI 1 showed significant increases 24 hours after PTCA and t PA showed significant increases 4 and 24 hours after PTCA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of u PA , t PA , PAI 1 and PAI 2 antigen as well as functional u PA were assessed in tissue homogenates from 20 chronic venous ulcers , six actively healing venous ulcers and five traumatic wounds . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Neither the morphology of the cells nor the secretion of prostacyclin ( PGI 2 ) , von Willebrand factor ( vWF ) , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) was affected by the crosslink density of the collagen substrate . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Since little is known about the evaluation of markers of haemostatic and fibrinolytic systems in other adrenal disorders , we studied plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( t PA ) , fibrinogen and von Willebrand factor antigen ( vWF Ag ) levels in 11 patients with Cushing ' s syndrome and in 12 patients with adrenal incidentaloma . ^^^ In patients with Cushing ' s syndrome mean PAI 1 , t PA and vWF Ag levels did not significantly differ from those found in 50 age and sex matched controls , while mean fibrinogen levels were significantly higher in patients ( 337 . 0+ / 39 . 1 mg / dl ) than in normal subjects ( 278 . 9+ / 8 . 4 mg / dl ) . ^^^ Patients with adrenal incidentaloma showed PAI 1 , t PA and vWF Ag mean levels superimposable to those in controls , while fibrinogen ( 319 . 7+ / 27 . 9 mg / dl ) was slightly , although not significantly , higher than in normals . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After five cycles of DNA shuffling and selection for t PA binding , analysis of 27 randomly picked clones revealed that PAI 1 mutants contained an average of 9 . 1 amino acid substitutions distributed over 114 different positions , which were preferentially located at the surface of the protein . ^^^ From the position of these amino acid residues in the 3D structure of PAI 1 and the effects of the corresponding monoclonal antibodies on t PA PAI 1 interaction , conclusions can be drawn with respect to this serpin serine proteinase interaction . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aims of the study were to determine the acute effect of angiotensin 2 and bradykinin on the local vascular release of tissue plasminogen activator ( t PA ) and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , and the endothelium derived haemostatic factor , von Willebrand factor ( vWf ) from the forearm . ^^^ RESULTS : Despite substantial rises in plasma angiotensin 2 concentrations ( P < 0 . 001 ) which caused pressor effects ( P < 0 . 003 ) at the highest dose , angiotensin 2 infusion did not affect local plasma t PA , PAI 1 or vWf concentrations . ^^^ In contrast , bradykinin caused substantial dose dependent increases in blood flow and t PA release ( > 100 ng / 100 ml of tissue / min ) in the infused forearm ( P < 0 . 001 for both ) without affecting plasma PAI 1 or vWf concentrations . ^^^ CONCLUSIONS : Despite high local concentrations with breakthrough of significant systemic effects , angiotensin 2 did not affect acute endothelial cell t PA , PAI 1 or vWf release in healthy men . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate the changes in tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and D dimer ( DD ) antigen plasma levels in acute myocardial infarction ( AMI ) patients after thrombolytic therapy with two different thrombolytic agents , rt PA or acetyl streptokinase and to find out any correlation between the plasma t PA , PAI 1 and DD levels with the infarct size as it is estimated from the peak of serum CPK levels . ^^^ The plasma antigen levels of t PA , PAI 1 and DD were measured by the enzyme immunoassay method ( Stago ) , in 57 consecutive patients ( M = 46 , F = 11 , mean age 55 . 6 + / 8 . 8 years ) and in 25 normal subjects ( M = 18 , F = 7 , mean age 54 . 0 + / 5 . 5 years ) . ^^^ During the acute phase of myocardial infarction the t PA , PAI 1 and DD antigen plasma levels were significantly higher than in healthy people . ^^^ There were no significant changes in the t PA , PAI 1 and DD plasma levels of the patients who were not treated with a thrombolytic agent . ^^^ We found a significant elevation of t PA ( p < 0 . 001 ) , PAI 1 ( p < 0 . 05 ) and DD ( p < 0 . 001 ) after 4 hours in comparison with the baseline ( at presentation , before therapy ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of protein C , protein S , antithrombin 3 , vWF , t PA and PAI 1 were determined before preparative chemotherapy , on the day of marrow infusion , and on days 7 , 14 and 21 . ^^^ Plasma levels of t PA , PAI 1 and vWF increased significantly in the early post transplant period compared to the baseline levels . ^^^ The mean plasma levels of t PA on day 7 ( P = 0 . 016 ) and PAI 1 on days 0 and 7 ( P = 0 . 016 , 0 . 032 ) were higher in the patients with VOD . ^^^ Levels of t PA and PAI 1 were significantly higher in the patients with VOD after BMT . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
This article describes these specific characteristics focussing on clot selectivity and PAI 1 resistance of the new mutants of wild type t PA and their possible clinical efficiency . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients with SIRS presented significantly higher values ( p < 0 . 001 ) for factors related to endothelial damage [ von Willebrand factor ( vWF ) , thrombomodulin , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen ] , hypercoagulability [ prothrombin fragment 1+2 ( F1+2 ) and thrombin antithrombin complexes ( TAT ) ] , and fibrinolysis ( D dimer and PAI activity ) with respect to the control group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total cholesterol ( TC ) , high density lipoprotein cholesterol ( HDL C ) , triglycerides ( TG ) , lipoprotein ( a ) ( Lp ( a ) ) , fibrinogen , plasminogen activator type 1 antigen ( PAI 1 ) , tissue plasminogen antigen ( t PA ) , von Willebrand factor ( vWF ) , and plasma total homocysteine ( tHcy ) were measured before and 12 weeks after the start of the study in both groups . ^^^ PAI 1 and t PA concentrations were also reduced by 21 % ( P : < 0 . 01 ) and 9 % ( P : < 0 . 05 ) respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In sixty eight peri and postmenopausal women qualified for hormone replacement therapy under study some coagulation and fibrinolysis parameters were measured in serum platelet count , prothrombin time , prothrombin ratio , INR , APTT , thrombin time ( TT ) , fibrinogen , D dimers , TAT , factor 7 , activity of antithrombin 3 , activity of protein C and protein S , PAI 1 and t PA concentration . ^^^ Impaired fibrinolysis ( low t PA , high PAI 1 ) together with the rise in activity of factor 7 can contribute to the development of ischaemic heart disease in menopausal women . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Total cholesterol and tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and t PA PAI 1 complex were reduced . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The enhancement by fucoidan 6 AH or by CNBr Fbg of the in vitro activation of Glu Plg by t PA was reversed by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
When patients with hyperhomocysteinemia were compared to patients without hyperhomocysteinemia , no significant differences in t PA ( antigen 9 . 2+ / 5 . 5 microg / L and 9 . 7+ / 4 . 7 microg / L , respectively ; activity 1 . 3+ / 0 . 5 IU / mL and 1 . 3+ / 0 . 7 IU / mL , respectively ) and PAI 1 ( antigen 19 . 3+ / 17 . 5 microg / L and 22 . 6+ / 20 . 4 microg / L , respectively ; activity 15 . 0+ / 12 . 6 and 15 . 8+ / 13 . 3 IU / mL , respectively ) were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
They produce antithrombotic and thrombotic factors such as t PA and PAI 1 and respond to TNFalpha , an important factor correlated with the inflammatory process by modifying growth characteristics by producing cytokines such as GM CSF by expressing ICAM 1 on the surface and by producing large amounts of nitric oxide and endothelin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of tissue and urokinase plasminogen activator ( t PA and uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and type 2 ( PAI 2 ) mRNA and protein were assessed by quantitative reverse transcriptase polymerase chain reaction ( Q RT PCR ) and ELISA , respectively . ^^^ TGF beta 1 downregulated , dose dependently t PA mRNA and protein to about 50 % of control values ( p = 0 . 0010 ) , and doubled PAI 1 protein production ( p = 0 . 0008 ) compared to untreated controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A 5 microg / kg bolus of tissue plasminogen activator ( t PA ) was infused into 11 healthy subjects followed by measurement of t PA activity and antigen , PAI 1 activity and antigen , t PA / PAI 1 complex , plasmin / antiplasmin ( PAP ) complex and D dimer over 4 h . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Tissue plasminogen activator ( t PA ) , its endothelial inhibitor ( PAI 1 ) , plasmin : antiplasmin complexes ( PAPc ) , modified antithrombin 3 ( ATM ) , D dimer ( D D ) and fibrinogen ( FIB ) were measured in 36 patients with chronic atrial fibrillation . ^^^ RESULTS : At baseline , patients with atrial fibrillation showed significantly higher plasma levels of PAI 1 , ATM , D D and FIB levels ( p < 0 . 05 ) than controls , and no differences in t PA and PAPc concentrations . ^^^ Levels of t PA , PAI 1 , ATM and D D decreased significantly under anticoagulant therapy , whereas FIB levels were not significantly modified . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In these subjects , the following parameters were established : euglobulin lysis time ( ELT ) , the concentration of tissue plasminogen activator antigen ( t PA Ag ) , the concentration of urokinase plasminogen activator antigen ( u PA Ag ) , the activity of plasminogen activator inhibitor type 1 ( PAI 1 ) , the concentration of plasmin antiplasmin complex ( PAP ) and fibrinogen / fibrin degradation products ( FDP ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activators ( t PA and u PA ) and plasminogen activators inhibitors ( PAI 1 and PAI 2 ) in some myeloproliferative syndromes . ^^^ In the study we aimed to evaluate fibrinolysis system in blood plasma of the patients with selected myeloproliferative syndromes on the basis of examinations of plasminogen activators ( tissue t PA and urokinase type u PA ) and type 1 and type 2 plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^ In citrate venous blood , the following parameters were determined : concentrations of antigen t PA , u PA , PAI 1 , PAI 2 , concentration of plasmin alpha 2 antiplasmin complexes ( PAP ) determined with the use of ELISA technique , PAI 1 activity with amidolytic method , euglobulin lysis time ( ELT ) according to Kowarzyk Buluk and fibrinogen / fibrin degradation products ( FDP ) concentration with the use of Merskey ' s method . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In addition , several plasma parameters , like PAI 1 , t PA , D dimers , prothrombin fragment F 1 + 2 , fibrinogen , ATIII activity , troponin 1 and CK MB , as well as platelet count were determined at each time point . ^^^ In these patients , fibrinolysis parameters tended to be depressed ( with increased PAI 1 dominating over elevated t PA ) on the first day after the operation , and no significant differences with regard to fibrinogen , prothrombin fragment F 1 + 2 , troponin 1 and platelet count . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Kaolin partial thromboplastin time ( KPTT ) , prothrombin time ( PT ) , fibrinogen content , vWF content were analyzed , and tissue plasminogen activator ( t PA ) activity , plasminogen activator inhibitor ( PAI ) activity , D dimer level were assayed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
At admission , patients showed significantly higher plasma levels of t PA , PAI 1 , and F 1 + 2 than controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Protein C was inversely associated with PAD prevalence ( odds ratio 0 . 33 , 95 % CI 0 . 18 0 . 61 , P=0 . 0004 ) . sTM was inversely associated with plasminogen , but no associations with t PA , PAI 1 , or D dimer were seen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
INTRODUCTION : Given that nonvalvular atrial fibrillation ( AF ) associated stroke can be either cardioembolic or atherothrombotic , we investigated the relationships between nonvalvular AF and hemostatic factors reflecting intrinsic thrombogenic and atherogenic potentials ( tissue plasminogen activator [ t PA ] antigen , plasminogen activator inhibitor 1 , and factor 8 activity ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Definition of t PA and PAI 1 activities can be used as prognostic markers of the fibrinolytic chain haemostasis system disfunction . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Different effects of oral and transdermal hormone replacement therapies on factor 9 , APC resistance , t PA , PAI and C reactive protein a cross sectional population survey . ^^^ Oral HRT use was associated with increased plasma levels of Factor 9 , activated protein C ( APC ) resistance , and CRP ; and with decreased levels of tissue plasminogen activator ( t PA ) antigen and plasminogen activator inhibitor ( PAI ) activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of t PA , PAI 1 , collagenase ( MMP 1 ) , tissue inhibitor of MMP ( TIMP 1 ) , plasmin antiplasmin ( PAP ) complexes and alpha 2 macroglobulin ( alpha 2 M ) were measured in plasma samples . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To further improve the efficacy of thrombolytic therapy , a PAI 1 resistant variant of t PA , TNK t PA , has been developed and is now available for acute myocardial infarction . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : In the present study , tissue plasminogen activator ( t PA ) and its main antagonist plasminogen activator inhibitor 1 ( PAI 1 ) were determined in 12 subjects with normal renal function ( group A ) [ serum creatinine ( Cr ) < 1 . 3 mg / dl ] , 24 patients with impaired renal function ( Cr 1 . 3 6 . 5 mg / dl ) ( group B ) and 22 patients with endstage renal disease ( ESRD ) on hemodialysis ( Cr > 6 . 5 mg / dl ) ( group C ) . ^^^ RESULTS : Plasma concentrations of PAI 1 and t PA antigen as well as the PAI 1 : t PA molar ratio were unchanged in group B as compared to group A . ^^^ However , in ESRD patients ( group C ) , t PA concentrations markedly decreased [ 13 . 7 + / 2 . 9 ng / ml vs . 32 . 8 + / 4 . 7 ng / ml ( group B , p < 0 . 01 ) and 35 . 4 + / 8 . 4 ng / ml ( group A , p < 0 . 01 ) ] while PAI 1 antigen concentrations remained in the control range . ^^^ Thus , the PAI 1 : t PA molar ratio significantly increased in group C patients [ 12 . 4 + / 4 . 0 vs . 6 . 0 + / 2 . 5 ( group B ; p < 0 . 01 ) and 4 . 5 + / 1 . 7 ( group A ; p < 0 . 01 ] . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
After Rg 1 infusion , the activity of t PA was found to be increased , so did the percentage of active type t PA , but the activity of PA 1 decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
On both treatment days , substance P caused dose dependent increases in blood flow and plasma t PA antigen and activity concentrations ( P < 0 . 001 for all ) but had no effect on plasma plasminogen activator inhibitor type 1 ( PAI 1 ) concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All subjects were investigated for IHD risk factors ( blood glucose , serum lipids , lipoprotein a ) with concurrent assays of coagulation fibrinolysis factors ( fibrinogen , antithrombin 3 , PAI 1 and t PA ) . ^^^ Compared to Groups A ( controls ) and B ( CABG with good course ) , these patients ( Group C ) had significant increases in Lp ( a ) , fibrinogen , LDL ch , PAI 1 and t PA and decreased HDL ch and AT 3 . 2 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concerning the fibrinolytic parameters , both OCs increased plasminogen activity , whereas no changes in PAI 1 , t PA , alpha 2 antiplasmin or fibrin degradation products were observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Endothelial derived ET 1 , t PA , PAI 1 , and platelet derived beta TG , PDGF , TGF beta were measured in 36 patients with primary RP , 14 patients with RP secondary to SSc and 30 age and sex matched controls . ^^^ RESULTS : A significative increase of ET 1 , t PA , PAI 1 , TGF beta , and beta TG were the most relevant changes in patients with RP secondary to SSc with respect to the controls . ^^^ Less relevant increases of t PA , PAI 1 , PDGF , and beta TG levels were observed in patients with primary RP vs controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this present study , we examined the expression of fibrinolytic factors , tissue type PA ( t PA ) and Type 1 PA inhibitor ( PAI 1 ) , in acute liver injury . ^^^ Plasma aspartate aminotransferase ( AST ) and alanine aminotransferase ( ALT ) activities were measured to verify the hepatocellular damage . t PA and PAI 1 gene expressions were measured by Northern blotting , and the cell type ( s ) expressing these genes was identified by in situ hybridization . t PA and PAI 1 levels were measured by enzyme linked immunosorbent assay ( ELISA ) . ^^^ Both t PA and PAI 1 gene expressions were induced by the acute liver injury , and plasma t PA and PAI 1 concentrations were also increased . ^^^ In situ hybridization studies demonstrated that the hepatocytes were the cells expressing t PA and PAI 1 genes during the acute liver injury . t PA was also augmented in the bile , whereas PAI 1 was decreased there . ^^^ CONCLUSIONS : t PA and PAI 1 gene expressions are induced in the hepatocytes of rats with acute liver injury . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Using an invasive technique , we studied the mean transit time , the net quantitative turnover rate , and the sites of synthesis and catabolism of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in healthy young volunteers in the fasting , steady state . ^^^ In conclusion , we demonstrated that active t PA and t PA antigen are catabolized and active PAI 1 produced in the splanchnic circulation in young healthy subjects during steady state . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activators ( tissue type ; t PA and urokinase type ; u PA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) are found in high concentrations in gingival crevicular fluid ( GCF ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of tissue plasminogen activator ( t PA ) antigen and activity , crosslinked fibrin degradation products ( D dimer ) , alpha 2 antiplasmin plasmin complex , and plasminogen activator inhibitor 1 ( PAI 1 ) antigen were measured . ^^^ No differences in the t PA antigen , PAI 1 antigen release , and plasmin inhibition by alpha 2 antiplasmin were apparent between the two groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CONCLUSIONS : The tumour itself is the origin of hypercoagulability ( TAT ) and fibrinolytic system inhibition ( PAI 1 ) ; the surgical procedure elicits an evident though moderate activation of the fibrinolytic system ( t PA and DD elevation ) . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To observe whether hyperglycemia and hyperinsulinemia , common findings in type 2 diabetes , acutely affect plasma fibrinolysis in vivo , we evaluated plasma fibrinolysis ( lysis of fibrin plates , free PAI 1 activity and t PA activity ) in the rat after a hyperglycemic euinsulinemic clamp ( n=8 ) , an euglycemic hyperinsulinemic clamp ( n=7 ) or a saline infusion ( n=15 ) . ^^^ These data show that in vivo both acute hyperglycemia and acute hyperinsulinemia can decrease plasma fibrinolytic potential and that this is due to increased plasma PAI 1 and decreased free t PA activities . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Components investigated were the fibrinolytic stimulator tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) and the antagonist . plasminogen activator inhibitor type one ( PAI 1 ) . ^^^ RESULTS : Immediately after 8 h of total anoxia and reoxygenation with HBO 2 ( for 1 . 5 h ) , the mean ( SEM ) concentrations of t PA , PAI 1 and uPA were significantly increased compared to the other groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : The children in whom the hemolytic uremic syndrome subsequently developed had significantly higher median plasma concentrations of prothrombin fragment 1+2 , tissue plasminogen activator ( t PA ) antigen , t PA plasminogen activator inhibitor type 1 ( PAI 1 ) complex , and D dimer than children with uncomplicated infection . ^^^ When the hemolytic uremic syndrome developed , the urinary concentrations of beta 2 microglobulin and N acetyl beta glucosaminidase rose significantly ( P=0 . 03 for both increases ) ; the plasma concentrations of t PA antigen , t PA PAI 1 complex , D dimer , and plasmin antiplasmin complex also increased significantly . ^^^ The concentration of t PA antigen correlated with that of the t PA PAI 1 complex in a linear regression model ( squared correlation coefficient , 0 . 80 ; P < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the present study , we used immunohistochemistry and in situ hybridization techniques to determine the localization of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators , type 1 plasminogen activator inhibitor ( PAI 1 ) and membrane receptor for u PA ( uPA R ) antigen and their sites of synthesis in renal thrombotic microangiopathy ( N = 10 ) as compared to acute tubular necrosis ( N = 5 ) and normal human kidneys ( N = 7 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Statins decreased mRNA levels for PAI 1 in endothelial and smooth muscle cells and increased mRNA levels for t PA in smooth muscle cells . ^^^ If the effects on the fibrinolytic system of vascular cells in vitro shown in this study are also operative in vivo one could speculate that by increasing t PA and decreasing PAI 1 at sites of vascular lesions statins might reduce fibrin formation and thrombus development . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin time ( PT ) , partial thromboplastin time ( PTT ) , fibrinogen ( FBG ) , platelets ( PLT ) , thrombin antithrombin complexes ( TAT ) , t PA Ag , t PA activity , plasminogen activator inhibitor 1 ( PAI 1 ) , and alpha 2 antiplasmin ( AP ) activities were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Gemfibrozil inhibited basal PAI 1 expression by 23 % ( p = ns ) and TGF beta induced PAI 1 expression by 52 % ( p = 0 . 017 ) whereas t PA and total protein synthesis was not affected . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor ( PAI 1 ) activities were measured . ^^^ Valsartan increased ( P < 0 . 05 ) acetylcholine induced relaxations , D dimer concentration and t PA activity , and reduced intimal thickening and PAI 1 activity in cholesterol fed rabbits . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of both tissue and urokinase type plasminogen activators ( t PA , u PA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of both tissue and urokinase type plasminogen activators ( t PA , u PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , fibrinogen , and D dimer were measured before and 20 minutes after the procedures . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Concentration of : tissue plasminogen activator antigen ( t PA : Ag ) , urokinase plasminogen activator antigen ( u PA : Ag ) , plasminogen activator inhibitor type 1 antigen ( PAI 1 : Ag ) ( ELISA ) , PAI 1 activity ( PAI 1 act . ) , euglobulin lysis time ( ELT ) ( Kowarzyk Buluk method ) , fibrinogen , fibrin degradation products ( FDP ) ( Merskey method ) in blood plasma were evaluated . ^^^ The results shows statistically higher concentrations of : t PA : Ag , PAI 1 : Ag , fibrinogen , and lower concentrations of u PA : Ag and elongated ELT in blood plasma patients with AO and DM in compare with healthy volunteers . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Subjects with an ultrasound confirmed deep venous thrombosis had prothrombin fragment 1+2 ( F 1+2 ) , tissue plasminogen activator ( t PA ) activity , plasminogen activator inhibitor ( PAI 1 ) activity , and t PA antigen levels determined before anticoagulation therapy . ^^^ However , the mean t PA antigen ( 10 . 7 plus minus 10 . 5 to 13 . 6 plus minus 13 . 5 ng / mL ; P = . 04 ) and PAI 1 ( 9 . 0 plus minus 8 . 1 to 13 . 2 plus minus 17 . 3 U / mL ; P = . 05 ) levels increased between the time of presentation and day 14 . ^^^ Percent recanalization was directly associated with initial t PA activity levels ( R = . 4 ; P = . 006 ) and inversely related to F 1+2 ( R = minus sign . 5 ; P = . 004 ) , t PA antigen ( R = minus sign . 5 , P = . 002 ) , and PAI 1 ( r = minus sign . 5 , P = . 001 ) levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the non diabetic women , changes in the haemostatic system included an increase in the procoagulant factors fibrinogen and Factor VIIc ; the concentration of active t PA increased , mainly because of decreased inhibition by PAI 1 . ^^^ We found no indications that insulin resistance is involved in the regulation of t PA and PAI 1 antigen levels , neither before nor during intake of OCs . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits plasminogen activators ( u PA and t PA ) by forming stable complexes endocytosed via a low density lipoprotein receptor superfamily member dependent mechanism . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits plasminogen activators ( u PA and t PA ) by forming stable complexes endocytosed via a low density lipoprotein receptor superfamily member dependent mechanism . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The hypertrophy observed in this model is , however , not related to changes in fibrinolytic parameters , as suggested by our finding that levels of t PA , u PA and PAI 1 antigen as well as t PA and u PA activity were not different in SC or GON adipose tissue extracts of both genotypes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In nonpregnant women s DD showed a good correlation with fibrinolytic parameters [ euglobulin lysis time ( ELT ) and type 1 inhibitor of tissue plasminogen activator ( PAI 1 ) act : P < . 01 ; tissue plasminogen activator ( t PA ) ag and PAI 1 ag : P < . 05 ] , confirming previous data , whereas in pregnant women no correlation was observed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Studies on the plasminogen activating system in gingival crevicular fluid ( GCF ) as well as gingival tissue are reviewed . t PA , u PA , PAI 1 and PAI 2 have all been detected in GCF . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelet count , fibrinogen level , prothrombin time , activated partial thromboplastin time , tissue plasminogen activator ( t PA ) activity , plasminogen activator inhibitor ( PAI ) activity , prothrombin fragment ( PF ) 1+2 level , and D dimer level were measured before surgery . ^^^ RESULTS : When compared with ruptured AAAs ( n = 37 ) , acutely symptomatic nonruptured AAAs ( n = 7 ) were associated with increased fibrinogen level ( P = . 033 ) , reduced activated partial thromboplastin time ( P = . 043 ) , increased t PA activity ( P = . 023 ) , reduced PAI activity ( P = . 005 ) , reduced PF 1+2 level ( P = . 001 ) , and reduced D dimer level ( P = . 005 ; all P values determined with Mann Whitney test ) . ^^^ The differences in t PA activity ( P = . 01 ) , PAI activity ( P = . 004 ) , and PF 1+2 level ( P = . 01 ) persisted in patients whose conditions were normotensive . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Sixty six acute cerebral infarction patients with blood stasis Syndrome , various vascular active factors such as tissue plasminogen activator ( t PA ) , the activity of plasminogen activator inhibitor ( PAI ) , the concentration of prostaglandin F 1 alpha ( 6 keto PGF 1 alpha ) etc . were determined . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinogen , factor 13 , ELT , FDP , Ag t PA levels , antigen concentration and PAI 1 activity were determined . ^^^ The patients demonstrated 3 fold higher t PA antigen level ( 13 . 1 ng / ml , a < 0 . 0001 ) and over 3 fold higher PAI 1 activity ( 26 . 7 AU / ml , a < 0 . 001 ) than healthy controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Covalent complexes of these derivatized PAI 1 species were made with the proteinases trypsin , LMW u PA , HMW u PA , and t PA . ^^^ Structural similarity of the covalent complexes formed between the serpin plasminogen activator inhibitor 1 and the arginine specific proteinases trypsin , LMW u PA , HMW u PA , and t PA : use of site specific fluorescent probes of local environment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma samples were evaluated for hypercoagulation markers ( FVIIa , F1+2 , TAT , D dimer ) , fibrinolysis proteins ( t PA , PAI 1 ) , and coagulation inhibitors ( protein C , AT ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin time ( PT ) , activated partial thromboplastin time ( aPTT ) , fibrinogen , factors 5 , 7 , 8 , 9 and 10 activities , vWF , antithrombin 3 ( AT 3 ) , protein C , protein S , tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor 1 ( PAI 1 ) , as well as common lipid variables , were measured . ^^^ Compared with control subjects , fibrinogen , factor 9 , vWF , AT 3 and PAI 1 were significantly increased in patients ( p < 0 . 05 , p < 0 . 0001 , p < 0 . 05 , p < 0 . 01 and p < 0 . 0001 ; respectively ) , whereas factor 10 and t PA were decreased ( p < 0 . 05 ) . ^^^ FT 4 , FT 3 and TSH did not correlate with fibrinogen , vWF , AT 3 , t PA , or PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) antigen , t PA activity , plasminogen activator inhibitor 1 ( PAI 1 ) activity and plasma renin activity ( PRA ) were measured at the end of a 2 week run in period on the control diet and after each treatment period . ^^^ Losartan significantly lowered t PA antigen levels ( 1 . 8 ng / mL , P = 0 . 045 ) , but had no effect on t PA or PAI 1 activities . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Swapping the 37 loop of TSV PA for either that of t PA or that of u PA also increased dramatically the rate of inactivation by PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Ang 2 increased PAI 1 and tissue plasminogen activator ( t PA ) release , while its metabolite angiotensin ( 1 7 ) ( Ang ( 1 7 ) ) amino acid fragment decreased them . ^^^ Angiotensin Type 1 ( AT 1 ) receptor antagonist , L 158 , 809 ( L 1 ) , and Ang ( 1 7 ) receptor antagonist , ( D Ala ( 7 ) ) angiotensin I / II ( 1 7 ) ( D Ala ) , decreased PAI 1 and t PA release ; angiotensin Type 2 ( AT 2 ) antagonist , PD 123 , 319 ( PD ) , however , did not have any effects on the release of PAI 1 and t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Thirteen age and gender matched sibling pairs of Type 2 ( non insulin dependent ) diabetic patients , one hyperglycaemic , one normoglycaemic ( fasting plasma glucose at screening 6 . 0 7 . 7 mmol . l ( 1 ) and < 6 . 0 mmol . l ( 1 ) , respectively ) were assessed for plasminogen activator inhibitor antigen ( PAI 1 ) , tissue plasminogen activator antigen ( t PA ) , fibrinogen , Factor 7 and Factor VIII / von Willebrand factor antigen . ^^^ Hyperglycaemic siblings had increased PAI 1 antigen , geometric mean ( i . q . r . ) : 26 . 3 ( 15 . 1 45 . 6 ) vs 11 . 1 ( 2 . 1 23 . 3 ) ng / ml , p=0 . 0002 , similar t PA antigen , mean ( SD ) 9 . 5 ( 4 . 3 ) vs 7 . 4 ( 2 . 5 ) ng / ml , p=0 . 2 and fibrinogen 2 . 2 ( 0 . 3 ) vs 2 . 3 ( 0 . 6 ) g / l , p=0 . 5 , and reduced % S 66 . 3 ( 30 . 5 ) vs 82 . 9 ( 25 ) , p=0 . 04 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESEARCH DESIGN AND METHODS : Von Willebrand factor ( vWF ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and thrombomodulin were studied as markers of endothelial dysfunction in a population based study of elderly subjects with normal glucose tolerance ( NGT ) or IGT and type 2 diabetes . ^^^ Increased levels of vWF antigen , t PA antigen , and PAI 1 activity were seen in the IGT and diabetic group compared with the NGT group . ^^^ Fasting blood glucose levels correlated with vWF antigen , t PA antigen , and PAI 1 activity , whereas urine albumin excretion correlated with TFPI activity , vWF antigen , and PAI 1 activity . ^^^ Serum insulin levels correlated strongly not only with vWF antigen and t PA antigen but also with PAI 1 activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PURPOSE : The serine proteases tissue plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) and their inhibitor , plasminogen activator inhibitor ( PAI ) 1 , regulate a variety of processes involved in tissue morphogenesis and differentiation . ^^^ The authors investigated whether expression of t PA , u PA , and PAI 1 in human retinal glial cells ( HRGCs ) is influenced by exposure to transforming growth factor ( TGF ) beta , a cytokine that regulates the proliferation and differentiation of cells . ^^^ METHODS : The extracellular release of t PA , u PA , and PAI 1 was measured by enzyme linked immunosorbent assay ( ELISA ) in the supernatant of HRGC cultures , under basal conditions and after stimulation with TGF beta at various concentrations ( 2 , 5 , 10 , or 20 ng / mL ) . ^^^ RESULTS : Under basal conditions , HRGCs secreted considerable amounts of t PA and PAI 1 . ^^^ Stimulation with TGF beta resulted in increased synthesis of t PA and PAI 1 protein in a time and dose dependent manner . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : Data about the effects of smoking on thrombo hemostatic factors ( tissue factor [ TF ] and tissue factor pathway inhibitor [ TFPI 1 ] ) are limited and on fibrinolytic factors ( tissue plasminogen activator [ t PA ] and plasminogen activator inhibitor 1 [ PAI 1 ] ) are debatable . ^^^ After the incubation , basal NO , t PA , PAI 1 , TF , TFPI 1 production , and substance P ( SP ) stimulated NO , t PA , and PAI 1 production were determined . ^^^ HUVECs treated with smokers ' serum showed lower basal ( P < 0 . 02 ) and SP stimulated ( P=0 . 059 ) t PA production but similar basal and stimulated PAI 1 production ( P=0 . 9 and P=0 . 6 ) compared with nonsmokers . ^^^ Basal t PA / PAI 1 molar ratio was significantly reduced in smokers ( P < 0 . 005 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma plasminogen activator inhibitor 1 ( PAI 1 ) levels were higher in mice on the HFD as compared to the SFD ; they were comparable in extracts of s . c . or GON adipose tissue , whereas at different time points tissue type ( t PA ) and urokinase type ( u PA ) plasminogen activator activity was somewhat lower in the adipose tissues of mice on HFD . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The concentrations of t PA , u PA , PAI 1 and PAI 2 were measured by ELISA . ^^^ Mean concentrations of t PA , u PA , PAI 1 were lower in Group 2 ( 5 . 69 ng / ml vs 15 . 7 ; 0 . 46 ng / ml vs 0 . 7 ; 16 . 82 ng / ml vs 26 . 16 ng / ml ) or nearly equal for PAI 2 ( 343 . 53 ng / ml vs 341 . 02 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To assess the effects of pharmacological interruption of the renin angiotensin system on the fibrinolysis , tissue plasminogen activator antigen ( t PA ) , plasminogen activator inhibitor 1 antigens ( PAI 1 ) and neurohormones , such as plasma renin activity , norepinephrine , angiotensin 2 ( AII ) and 4 ( AIV ) concentrations , were measured in 60 hypertensives . ^^^ In the quinapril group , there was a significant change in t PA ( p < 0 . 001 ) without changes in PAI 1 . ^^^ In the losartan group , free PAI 1 and total PAI 1 ( p < 0 . 05 for free PAI 1 and p < 0 . 04 for total PAI 1 ) were significantly increased without a change in t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) have opposing effects on thrombi . ^^^ Hence , the extent of the clot , the size of the infarct and outcome of patients could depend on t PA and PAI 1 levels . ^^^ In an effort to elucidate the pathophysiologic basis of circadian variation of AMI , we investigated the presence of a possible corresponding circadian variation in the levels of endogenous t PA and PAI 1 in patients diagnosed to have AMI and the effects of hypertension , diabetes and site of the infarct on these levels . ^^^ We estimated the levels of t PA and PAI 1 in platelet poor plasma of 42 patients with AMI on admission , using the enzyme linked immunosorbant assay . ^^^ Although not statistically significant , patients having an AMI in the morning hours had the highest t PA : PAI 1 ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) , fibrinogen and insulin were measured in 43 patients 3 years after they had undergone the Magenstrasse and Mill ( MM ) procedure and in 43 morbidly obese ( MO ) patients . ^^^ Mean plasma PAI 1 was 61 ng / ml in the MO group compared to 30 ng / ml in the MM group ( p < 0 . 0001 ) ; mean plasma t PA was 10 ng / ml in the MO group compared to 7 ng / ml in the MM group ( p < 0 . 001 ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) , fibrinogen and insulin were measured in 43 patients 3 years after they had undergone the Magenstrasse and Mill ( MM ) procedure and in 43 morbidly obese ( MO ) patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the fibrinolytic system , the serpin PAI 1 negatively regulates the enzymatic activity of both single chain and two chain t PA ( sct PA and tct PA ) . ^^^ The interactions between t PA and NSP contrast with the formation of long lived , physiologically irreversible acyl enzyme complexes between t PA and PAI 1 , suggesting that the physiologic effect of t PA NSP interactions may be more complex than previously thought . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We conclude that the secretion of t PA antigen from arterial endothelium of healthy subjects is substantial , whereas the arterial wall is not an important source of PAI 1 in vivo . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We assessed lipids , body mass index ( BMI ) and new risk factors : lipoprotein ( a ) Lp ( a ) , apolipoprotein A 1 ( apo A 1 ) and apolipoprotein B ( apo B ) , homocysteine ( Hcy ) , fibrinogen ( FB ) , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Compared to lean subjects , obese subjects exhibited higher plasma levels of all the studied parameters ( except for TGFbeta 1 ) whereas in adipose tissue only PAI 1 , t PA and TGFbeta antigen levels differed . ^^^ In the obese population , plasma PAI 1 levels were weakly associated with circulating TNF , and this relationship disappeared after adjustment for plasma t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The reduced inhibitory activity of PAI 1 against t PA but not u PA suggested that the mechanism of loop insertion is sensitive to the intramolecular interactions of one or more tryptophan residues . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma fibrinogen , red cell aggregation ( RCA ) , viscosity , factor 7 activity , plasminogen activator inhibitor ( PAI ) activity , tissue plasminogen activator antigen ( t PA ) , plasma cholesterol , triglyceride and insulin . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples for plasminogen activator inhibitor 1 ( PAI 1 ) antigen and tissue plasminogen activator ( t PA ) antigen were obtained at baseline in all patients and control subjects . ^^^ RESULTS : The mean basal plasma level of PAI 1 of hypertensive patients was higher than those of the normotensive control group ( 60 . 98 + / 4 . 2 vs . 24 . 09 + / 1 . 61 ng / ml , p < 0 . 01 ) The mean basal t PA level was similar in the hypertensive and control subjects ( 7 . 49 + / 0 . 65 vs . 8 . 78 + / 0 . 92 ng / ml , p > 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We examined the diurnal pattern in Plasminogen Activator Inhibitor type 1 ( PAI 1 ) activity and Plasminogen activator ( t PA ) in relation to the 4G / 5G polymorphism in the promoter of the PAI 1 gene . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 antigen and activity levels , t PA antigen and activity levels and plasmin antiplasmin ( PAP ) complexes were measured on days 1 , 3 , 8 , 15 , 17 and 22 of the experiment period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Measurement of platelet number , plasma levels of fibrinogen , tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) activity , and fibrin fragment D dimer . ^^^ Comparison of the values in the third trimesters of normal and pre eclamptic pregnancies showed similar values for the fibrinogen and platelet counts , and higher values of t PA ( almost twice normal median value ; P < 0 . 0001 ) , PAI 1 and D dimer in the pre eclamptic women . t PA correlated positively and significantly with the degree of proteinuria in pre eclamptic women ( r = 0 . 575 , P = 0 . 0002 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Finally , we showed that TGF alpha , by an ERK dependent mechanism , stimulates the astrocytic expression of PAI 1 , a t PA inhibitor , which mediates the neuroprotective activity of TGF alpha against NMDA mediated excitotoxic neuronal death . ^^^ Taken together , we indicate that TGF alpha rescues neurons from NMDA induced excitotoxicity in mixed cultures through inhibition of t PA activity , involving PAI 1 overexpression by an ERK dependent pathway in astrocytes . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The present study has dealt with the role of the t PA inhibitor , PAI 1 , in the neuroprotective activity of the cytokine TGF beta 1 and focused on the transduction pathway involved in this effect . ^^^ This t PA inhibitor , PAI 1 , protected neurons against NMDA induced neuronal death by modulating the NMDA evoked calcium influx . ^^^ Overall , this study underlines the critical role of the t PA / PAI 1 axis in the regulation of glutamatergic neurotransmission . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We could demonstrate that HACM , isolated from pieces of myocardial tissue by mechanical dispersion and characterized by positive immunostaining for the cardiac markers troponin 1 , tropomyosin , cardiotin and myocardial muscle actin , in vitro express PAI 1 and tissue type PA ( t PA ) whereas u PA was not detectable in these cells . ^^^ Similar changes were observed in PAI 1 transcript levels after cytokine treatment . t PA production in HACM was not affected by these agonists . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Von Willebrand factor , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and thrombomodulin were also measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Age and sex adjusted HOMA was correlated to waist hip ratio ( r = 0 . 38 , p = 0 . 0001 ) , triglycerides ( r = 0 . 22 , p = 0 . 03 ) , systolic blood pressure ( r = 0 . 21 , p = 0 . 04 ) , tissue plasminogen activator ( t PA ) ( r = 0 . 22 , p = 0 . 04 ) ; plasminogen activator inhibitor 1 ( PAI 1 ) ( r = 0 . 26 , p = 0 . 02 ) ; fibrinogen ( r = 0 . 25 , p = 0 . 02 ) ; and factor 7 antigen ( r = 0 . 21 , p = 0 . 06 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum leptin , glucose , insulin , lipid profile , sex hormone binding globulin ( SHBG ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and fibrinogen were all measured . ^^^ In the obese group , leptin showed a positive correlation with BMI ( P < 0 . 001 ) , insulin ( P < 0 . 001 ) , triglycerides ( P < 0 . 05 ) , PAI 1 ( P < 0 . 05 ) and t PA ( P < 0 . 05 ) , and correlated negatively with SHBG ( P < 0 . 01 ) , apolipoprotein A 1 ( P < 0 . 05 ) and high density lipoproteins cholesterol ( HDL C ) ( P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
PAI 1 and tissue plasminogen activator ( t PA ) showed significant genetic correlation with sBP and with each other . ^^^ Pleiotropy was demonstrated between fibrinogen and PAI 1 , t PA and FVII . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : When different concentration of 17 beta estrodial including 10 ( 10 ) mol / L ( the group E 1 ) , 10 ( 8 ) mol / L ( the group E 2 ) , 10 ( 6 ) mol / L ( the group E 3 ) were treated in original cultured HUVEC , the changes of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 1 ) were measured by spectrophotometric assay , and the level of message RNA were examined by in situ hybridization . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of t PA were normal and PAI 1 was mildly elevated in group A . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Fifty seven cases of AICVD were grouped and treated with heparin and Xueshuantong ( XST ) respectively , and the levels of prothrombin time ( PT ) , activated partial thromboplastin time ( APTT ) , fibrinogen ( FIB ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) and activated protein C ( APC ) were determined before and after treatment . ^^^ In the heparin group , level of PT and APTT prolonged , FIB decreased , t PA activity elevated and PAI activity lowered , and APC unchanged . ^^^ In the XST group , same changes in FIB , t PA and PAI were shown but with APC increased , and PT and APTT unchanged . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Changes of tissue plasminogen activator ( t pA ) and plasminogen activator inhibitor activity ( PAI ) as well as the intestinal adhesion were investigated . ^^^ RESULTS : Different dosage of CTOL produced protective effects on the postoperative intestinal adhesion accompanied by significant enhancement of t PA activity and pronounced decreasement of PAI activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) may be involved in the pathogenesis of peptic ulcers through suppression of fibrinolysis . ^^^ This study was designed to investigate associations of t PA and PAI 1 genes with clinical features of the patients with bleeding gastric ulcers . ^^^ We used polymerase chain reaction and endonuclease digestion to genotype for 4G / 5G polymorphism in the promoter region of the PAI 1 gene and the Alurepeat insertion / deletion ( I / D ) polymorphism in intron h of the t PA gene . ^^^ The genotype distributions of both the t PA and PAI 1 genes did not differ between the patient and control groups . ^^^ Our data suggest that t PA gene polymorphism is associated with duodenal ulcers , and that the PAI 1 gene may be a risk factor leading to recurrent bleeding after initial hemostasis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The fibrinolytic activity and protein levels of tetranectin , and t PA and its endogenous inhibitor PAI 1 , were subsequently shown to change significantly in both skeletal muscle and serum in response to exercise training . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Plasminogen activators ( t PA and u PA ) and their inhibitors ( PAI 1 and PAI 2 ) secretions were assayed in cultures of epithelial , stromal , and trophoblast cells . ^^^ Estradiol ( E 2 ) enhanced t PA production in stromal cells and PAI 1 production in epithelial cells . ^^^ Trophoblasts produced PAI 1 , PAI 2 , and small quantities of t PA and u PA , none of which were notably influenced by E 2 or P 4 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Euglobulin lysis time , t PA antigen ( t PA : Ag ) and activity , PAI 1 antigen and activity plasma levels were assayed before and 20 minutes after VO ; the Alu repeat I / D polymorphism was determined by PCR . ^^^ Defective fibrinolysis was due to reduced t PA release in 40 patients ( t PA group ) and to PAI 1 excess in 42 patients ( PAI group ) . ^^^ The t PA : Ag increase after VO ( 20 / 0 minute levels ratio adjusted for hematocrit ) was considerably higher both in controls and in PAI group patients carrying the 1 allele than in the DD genotype carriers ( 2 , ID , DD : 3 . 77+ / 0 . 62 , 3 . 43+ / 0 . 44 , 2 . 06+ / 0 . 32 in controls , and 3 . 67+ / 0 . 23 , 2 . 80+ / 0 . 50 , 1 . 62+ / 0 . 29 in PAI group , respectively ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
For PAI 1 Ag and t PA Ag they were 20 % and 26 % . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Interestingly , IL 6 was also positively associated with t PA / PAI 1 complexes after adjustment for BMI and other anthropometric variables . ^^^ Leptin was positively correlated with PAI 1 activity and antigen ( r = 0 . 32 , p = 0 . 006 and r = 0 . 37 , p < 0 . 001 , respectively ) and negatively with t PA activity ( r = 0 . 27 , p = 0 . 03 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Free T 4 , FT 3 and TSH did not correlate with fibrinogen , vWF , AT 3 , t PA , or PAI 1 . aPTT correlated inversely with t PA activity and positively with protein C activity . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As `` CAD risk factors ' ' were determined the levels of fibrinogen , acute phase proteins orosomucoid and C reactive protein , t PA Ag , PAI 1 Ag and soluble adhesion molecules E selectin , P selectin and ICAM 1 in the peripheral blood serum or plasma . ^^^ RESULTS : Renal transplant recipients showed higher BMI ( p < 0 . 001 ) and levels of fibrinogen ( p < 0 . 001 ) , t PA Ag ( p = 0 . 007 ) and PAI 1 Ag ( p < 0 . 001 ) , acute phase protein orosomucoid ( p < 0 . 001 ) and higher level of soluble P selectin ( p = 0 . 038 ) . ^^^ CONCLUSION : Our study has demonstrated renal graft recipients with good kidney function already show significantly raised levels of `` CAD risk factors ' ' fibrinogen , acute phase reactant orosomucoid , t PA Ag , PAI 1 Ag and sP selectin . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinolysis was evaluated before and after venous occlusion , together with euglobulin lysis time , t PA , and PAI 1 titers . ^^^ However , the more specific fibrinolytic markers t PA and PAI 1 did not differ significantly between the two study groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Correlation of repeated measurements of t PA and PAI 1 concentrations and activities from the same sample using the same ELISA kit was high ( r = 0 . 93 0 . 99 , p < 0 . 01 ) . t PA activities and concentrations between the groups correlated poorly ( r= 0 . 60 and 0 . 66 , p < 0 . 01 ) while no correlation at all was seen for PAI 1 concentration and activity between the groups ( r = 0 . 6 and 0 . 1 , p = 0 . 2 and 0 . 9 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BMI was calculated , and the following measurements were taken : International Normalized Ratio , antithrombin 3 , tissue plasminogen activator ( t PA ) activity , t PA antigen , plasma fibrinogen level , factor 7 , Plasminogen activator inhibitor ( PAI ) 1 activity and antigen and metabolic parameters : fasting insulin and glucose , total cholesterol , HDL cholesterol , LDL cholesterol and triglycerides . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) is the primary inhibitor of both tissue and urokinase type plasminogen activators ( t PA , u PA ) and is thus a primary regulator of plasminogen activation and possibly of extracellular proteolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To measure the concentration of D dimer ( DD ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen ( PLG ) activity in plasma and cerebrospinal fluid in patients with acute cerebral infarction and to investigate their clinical significance . ^^^ METHODS : The concentrations of D dimer , t PA , and PAI 1 in plasma and cerebrospinal fluid in patients were measured by enzyme linked immunosorbent assay ( ELISA ) . ^^^ RESULTS : The concentrations of D dimer , t PA and PAI 1 in cerebrospinal fluid and plasma in patients with acute cerebral infarction were much higher than those of normal subjects ( P < 0 . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Real time PCR was used to demonstrate quantitatively that the levels of t PA transcripts were higher than those of its primary regulator [ ie , plasminogen activator inhibitor 1 ( PAI 1 ) ] in the Pa samples . ^^^ Immunologic and functional assays extended these observations by demonstrating that levels of t PA antigen were higher than PAI 1 antigen , which resulted in the detection of free t PA activity . ^^^ This contrasted with the fibrinolytic balance of matched samples of aorta ( n = 6 ) in which PAI 1 transcripts and antigen values were higher than the corresponding t PA values , and only M ( r ) 110 kDa t PA PAI 1 complexes could be detected in functional assays . ^^^ Pa endothelial cell conditioned media revealed increased t PA / PAI 1 antigen ratios . ^^^ Taken together , our data indicate that the balance between t PA and PAI 1 is shifted in human main Pa to favor net PA activity . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the study was to evaluate levels of several new atherosclerosis risk factors ( lipoprotein ( a ) ( Lp ( a ) ) , apolipoprotein A 1 ( Apo A 1 ) , apolipoprotein B ( Apo B ) , homocysteine ( Hcy ) , fibrinogen ( FB ) , tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor type 1 ( PAI 1 ) ) in children and adolescents with traditional risk factors ( obesity , hypertension , diabetes ) . ^^^ Lp ( a ) , Apo A 1 and Apo B levels were estimated by use of immunoturbidimetric methods ; total Hcy , FB , t PA and PAI 1 were estimated by use of immunoenzymatic methods . ^^^ Significantly higher t PA level was found in groups A ( 9 ng / ml ) and B ( 9 . 7 ng / ml ) versus 7 . 3 ng / ml in controls , and PAI 1 level was significantly higher in the total study group ( 62 . 3 ng / ml ) and in groups A ( 73 . 8 ng / ml ) , B ( 78 ng / ml ) and C ( 73 ng / ml ) versus 42 . 4 ng / ml in the control group . ^^^ Correlation analysis showed significant relationship between body mass index ( BMI ) and Apo B , Hcy , FB , t PA and PAI 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Mean fasting insulin levels were higher among PCOS women ( p < 0 . 01 ) and were strongly correlated with PAI 1 antigen ( Ag ) ( r = 0 . 46 ) , PAI 1 activity ( r = 0 . 43 ) , and tissue plasminogen activator ( t PA ) ( r = 0 . 5 ) . ^^^ Mean PAI 1 Ag , PAI 1 activity , and t PA levels were significantly elevated ( p = 0 . 003 , 0 . 001 , and 0 . 001 , respectively ) in PCOS . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood factors were D dimer , prothrombin 1 . 2 , tissue plasminogen activator ( t PA ) antigen , plasminogen activator inhibitor 1 ( PAI 1 ) , and inflammatory markers ( high sensitivity C reactive protein [ CRP ] , fibrinogen , and serum amyloid A [ SAA ] ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Fibrinolysis was assessed by measurement of tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor ( PAI 1 ) concentrations , whereas activation of thrombinogenesis was detected by F 1 + 2 prothrombin fragment concentration . ^^^ RESULTS : In children treated with cyclosporine A increased concentrations of t PA and PAI 1 were found ( p < 0 . 01 ) . ^^^ CONCLUSION : Children with remission of idiopathic nephrotic syndrome treated with cyclosporine A show increased concentration of t PA and PAI 1 and thrombinogenesis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coagulation parameters studied concerned prothrombin time ( PT ) , platelet count , the procoagulant system ( factors ( F ) 2 , FV , FVII , FVIII and fibrinogen ) , anticoagulant system ( AT 3 ) , fibrinolytic system ( t PA , PAI 1 ) as well as markers of coagulation factor activation ( TAT complexes , prothrombin fragment F 1 + 2 ) . ^^^ Levels of t PA and PAI 1 were not influenced by cell loaded BAL treatment . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of the study was to evaluate levels of selected new atherosclerosis risk factors ( Lp ( a ) , apo A 1 , apo B , homocysteine , fibrinogen ) and markers of fibrinolysis ( t PA and PAI 1 ) in children and adolescents with traditional risk factors ( obesity , hypertension , diabetes ) . ^^^ Lp ( a ) , apo A 1 and apo B levels were estimated by use of immunoturbidimetric methods , total homocysteine , fibrinogen , t PA and PAI 1 were estimated by use of immunoenzymatic methods . ^^^ Significantly higher t PA level was found in the obese group ( 9 micrograms / L ) and obesity with hypertension group ( 9 . 7 micrograms / L ) vs 7 . 3 mg / L in controls , and PAI 1 level was significantly higher in total study group ( 62 . 3 micrograms / L ) and groups of obese children ( 73 . 8 micrograms / L ) , obese and hypertensive ( 78 micrograms / L ) and hypertensive ( 73 micrograms / L ) vs 42 . 4 micrograms / L in control group . 28 % of study children had positive family history of cardiovascular diseases . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
To understand the mechanism of its beneficial effect , we studied the impact of DF on the expression of tissue factor ( TF ) and fibrinolytic proteins ( PAI 1 and t PA ) on endothelial cells . ^^^ Specifically , it dose dependently counteracted the LPS induced increase in PAI 1 levels and decrease in t PA activity expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In vitro , TGF beta 1 protects neurons against excitotoxicity by inhibiting the t PA potentiated NMDA induced neuronal death through a mechanism involving the up regulation of the type 1 plasminogen activator inhibitor ( PAI 1 ) in astrocytes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The biochemical markers ' levels in plasma for platelet activity [ soluble P selectin ( GMP 140 ) ] , states of thrombin generation [ antithrombin 3 ( AT 3 ) and protein C ( PC ) ] , fibrinolysis [ D dimer ( DD ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and FDP ] and von Willebrand factor ( vWF ) were determined from blood specimens obtained from the femoral veins and arteries and the right and left atria of 43 consecutive patients ( 20 with atrial fibrillation and 23 with sinus rhythm ) with mitral stenosis ( MS ) , undergoing percutaneous mitral valvuloplasty . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
DESIGN AND METHODS : We studied the effects of ATRA on procoagulant ( i . e . tissue factor , TF ) , fibrinolytic ( i . e . tissue plasminogen activator and inhibitor , t PA and PAI 1 ) and anticoagulant ( i . e . thrombomodulin , TM ) properties of HMEC 1 , compared to HUVEC . ^^^ ATRA increased t PA antigen without significantly affecting PAI 1 expression , and counteracted the TNFalpha induced t PA decrease in both types of EC . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : The mean depot specific expression of PAI 1 and t PA mRNA ( n = 42 ) in subcutaneous abdominal ( n = 21 ) , omental ( n = 10 ) and thigh ( n = 11 ) adipose tissue depots was examined . ^^^ Conditioned media were collected and enzyme linked immunosorbent assays performed for PAI 1 and t PA ( n = 12 ) antigen . ^^^ PAI 1 and t PA mRNA levels were also assessed . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In vitro , TGF beta 1 protects neurons against excitotoxicity by inhibiting the t PA potentiated NMDA induced neuronal death through a mechanism involving the up regulation of the type 1 plasminogen activator inhibitor ( PAI 1 ) in astrocytes 4 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In this paper , we have studied the contribution of the plasminogen activation system in the development of atherosclerosis by cross breeding apoE 3 Leiden mice , which have a human like lipid profile , with mice deficient in PAI 1 ( plasminogen activator inhibitor 1 ) , u PA ( urokinase plasminogen activator ) , and t PA ( tissue plasminogen activator ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured the hemostatic factors , fibrinogen , factor VIIc , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) , as well as glucose and insulin to calculate insulin resistance . ^^^ After adjustment for body mass index , site , and ethnicity , SHBG was correlated with PAI 1 ( partial r = 0 . 30 ) and t PA ( partial r = 0 . 12 ) . ^^^ Although testosterone was associated with t PA ( partial r = 0 . 13 ) and PAI 1 ( partial r = 0 . 07 ) , free androgen index was strongly correlated with t PA ( partial r = 0 . 18 ) and PAI 1 ( partial r = 0 . 26 ) . ^^^ SHBG , which influences the amount of bioavailable hormone , significantly modified the association of PAI 1 and t PA with insulin resistance . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : To elucidate the role of some haemostatic gene polymorphisms and environmental factors , we studied fibrinogen ( Fb ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue plasminogen activator ( t PA ) levels with respect to Fb G455A and PAI 1 4G / 5G gene polymorphisms in smokers and nonsmokers with essential hypertension . ^^^ Comparison of 4G allele carriers ( 4G / 4G homozygotes ) revealed significantly higher Fb ( p=0 . 045 ) , PAI 1 ( p=0 . 009 ) , and t PA levels ( p=0 . 007 ) in HT patients than controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Coronary net fluxes of total t PA antigen , active t PA and total PAI 1 antigen were determined at baseline and at 3 , 5 , 7 and 10 minutes of IPR infusion . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma t PA antigen was correlated with plasminogen activator inhibitor type 1 ( PAI 1 ) activity when the participants consumed all three diets ( Rs = 0 . 78 , P < 0 . 01 ; Rs = 0 . 76 , P < 0 . 01 ; Rs = 0 . 66 , P = 0 . 03 ; on the HSAFA , the LSAFA and the HUFA diet , respectively ) , although the diets did not affect the PAI 1 levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We hypothesized that elevated plasminogen activator inhibitor 1 ( PAI 1 ) activity could neutralize infused t PA or urokinase , resulting in lysis failure . ^^^ Active PAI 1 , active t PA and total t PA antigen were measured in 20 patients receiving t PA , and active PAI 1 was measured in four patients receiving urokinase for acute peripheral arterial thrombosis . ^^^ In the patients that failed to lysed their thrombi , > 95 % of infused t PA was neutralized by PAI 1 . ^^^ We conclude that elevated PAI 1 during acute peripheral arterial thrombolysis is associated with an increased risk of lysis failure due to reduced levels of circulating active t PA or urokinase . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Laboratory evaluation indicated excessive fibrinolysis with low plasminogen , low alpha 2 antiplasmin , undetectable plasminogen activator inhibitor type 1 ( PAI 1 ) activity , high tissue plasminogen activator ( t PA ) activity and high t PA antigen . ^^^ Circulating PAI 1 activity , t PA activity and antigen normalized by 3 months post transplant . ^^^ In short , the profound bleeding diathesis , as well as the imbalance in t PA and PAI 1 levels , corrected after liver transplantation . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prolonged euglobulin clot lysis time , elevated t PA antigen , PAI 1 antigen and activity , which all correlated with parameters of the plurimetabolic syndrome , were found in women with idiopathic VT , who were also clearly obese but not in women in whom oral contraceptives were the triggering factor for VT . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the impact of 30 minute walking exercise at 70 % VO2max on tissue plasminogen activator ( t PA ) Ag and plasminogen activator inhibitor type 1 ( PAI 1 ) Ag in obese sedentary males . ^^^ RESEARCH METHODS AND PROCEDURES : A controlled observational study of the effect of a 30 minute acute exercise bout at 70 % VO2max on plasma t PA antigen and PAI 1 antigen in 10 obese sedentary males matched for age , ethnic origin , and smoking status with 10 nonobese sedentary male controls . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The type 1 plasminogen activator inhibitor ( PAI 1 ) , the primary inhibitor of both tissue type and urokinase type plasminogen activators ( t PA , u PA ) , is the primary regulator of plasminogen activation and possibly of extracellular proteolysis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following fibrinolytic parameters were measured : tissue type plasminogen ( t PA ) antigen and activity , plasminogen activator inhibitor 1 ( PAI 1 ) antigen and activity , and euglobulin clot lysis time ( ECLT ) . ^^^ No difference was found between the samples obtained from the right atrium and the left ventricle with respect to t PA antigen : 8 . 1 ( 6 . 7 11 . 3 ) vs 8 . 4 ( 5 . 9 11 . 0 ) ng / ml ; t PA activity : 92 ( 5 680 ) vs 62 ( 32 696 ) IU / ml ; PAI 1 antigen : 8 . 4 ( 5 . 5 14 . 3 ) vs 8 . 7 ( 6 . 2 13 . 1 ) ng / ml ; and ECLT : 5 . 5 ( 4 . 1 9 . 1 ) vs 5 . 6 ( 4 . 1 8 . 5 ) 1 000 / min . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Several variables of the fibrinolytic system such as plasminogen activator inhibitor 1 ( PAI 1 ) antigen and activity , tissue plasminogen activator ( t PA ) antigen and activity as well as circulating t PA / PAI 1 complexes were determined . ^^^ RESULTS : At baseline , the body mass index but not blood pressure was positively associated with PAI 1 antigen ( r=0 . 314 , p < 0 . 01 ) and PAI 1 activity ( r=0 . 425 , p < 0 . 01 ) but negatively with t PA activity ( r= 0 . 187 , p < 0 . 05 ) . ^^^ However , there was no significant effect of candesartan on all parameters of the fibrinolytic system under investigation , i . e . circulating PAI 1 antigen , PAI 1 activity , t PA antigen , t PA activity and t PA / PAI 1 complexes . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma levels of the thrombin activation system ( thrombin antithrombin complex [ TAT ] , tissue factor pathway inhibitor [ TFPI ] , and prothrombin fragments 1+2 [ F1+2 ] ) and plasmin activation system ( tissue and urokinase type plasminogen activator [ t PA and u PA ] , plasminogen activator inhibitor 1 [ PAI 1 ] , and D dimer ) were measured with enzyme linked immunosorbent assay kits before angiography . ^^^ RESULTS : Elevated levels of TAT ( 7 . 2 + / 6 . 0 microg / L ) , F1+2 ( 1 . 8 + / 1 . 0 nmol / L ) , TFPI ( 179 . 1 + / 131 . 0 ng / mL ) , PAI 1 ( 95 . 4 + / 54 . 6 ng / mL ) , t PA ( 10 . 6 + / 8 . 8 ng / mL ) , and u PA ( 2 . 6 + / 0 . 9 ng / mL ) were found in patients with UA / NSTEMI . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
INTERVENTIONS : We characterized the expression of tissue type and urokinase type plasminogen activator ( t PA and u PA ) as well as plasminogen activator inhibitor ( PAI ) 1 and PAI 2 in human endothelial cells ( EC ) from the microvascular pulmonary circulation ( HMVEC L ) and compared it with that of EC from pulmonary artery ( HPAEC ) and umbilical vein ( HUVEC ) under baseline conditions and upon stimulation with either tumor necrosis factor alpha or lipopolysaccharide . ^^^ HPAEC were characterized by significantly higher baseline expression of t PA and PAI 2 compared with HUVEC and HMVEC L . ^^^ In all EC , stimulation with tumor necrosis factor alpha and lipopolysaccharide increased the expression of PAI 1 , PAI 2 , and u PA and decreased t PA expression . ^^^ Microbial and inflammatory challenge provokes up regulation of PAI 1 and PAI 2 and down regulation of t PA in both macro and microvascular pulmonary EC , which may favor local fibrin deposition . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the concentration of selected haemostatic factors ( HFs ) : thrombin antithrombin complexes ( TAT ) , antithrombin ( AT ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and D dimers in carotid bifurcation plaques and to compare plaque composition in different subgroups of patients ( mainly those with symptomatic and asymptomatic carotid stenosis ) . ^^^ No significant differences were found between symptomatic and asymptomatic carotid plaques with respect to t PA , PAI 1 and D dimers concentration . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Because vascular tissue is a source of the endogenous fibrinolytic markers , and ACE inhibition in vascular tissue favorably influences the fibrinolytic system , the authors hypothesized that a high tissue penetrating ACE inhibitor would provide a more favorable reduction in plasminogen activator inhibitor 1 ( PAI 1 ) and an increase in tissue plasminogen activator ( t PA ) after AMI compared to a low tissue penetrating ACE inhibitor . ^^^ PAI 1 and t PA antigen ( ng / mL ) were measured at baseline and through 14 days of treatment . ^^^ There was no difference in baseline PAI 1 or t PA antigen between treatments . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
INTRODUCTION : Cardiopulmonary bypass ( CPB ) is associated with elevated tissue plasminogen activator ( t PA ) levels during CPB and increased plasminogen activator inhibitor 1 ( PAI 1 ) levels post operatively . ^^^ The goal of this study was to estimate the rate of t PA and PAI 1 secretion in vivo , before , during and after CPB . ^^^ MATERIALS AND METHODS : Estimated rates of t PA and PAI 1 secretion were based on measured levels of active and total t PA , and active and total PAI 1 , obtained before , during and after CPB from nine males , combined with a computer model of each patient ' s vascular system that continuously accounted for secretion , clearance , hemodilution , blood loss and transfusion . ^^^ RESULTS AND CONCLUSIONS : At baseline , the average t PA and PAI 1 secretion rates were 0 . 74+ / 0 . 33 and 1 . 28+ / 0 . 74 pmol / s , respectively . ^^^ Within 5 min of CPB initiation , t PA secretion increased six fold to 4 . 41+ / 2 . 58 pmol / s , while PAI 1 secretion was unchanged , resulting in a six fold increase in active t PA levels . t PA secretion remained elevated throughout CPB and into the early post operative period . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Components of the plasmin system including tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , and plasminogen activator inhibitors PAI 1 and PAI 2 are synthesised by airway cells , and inflammatory mediators affect their expression . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Treatment with all four OCs caused an increase in levels of fibrinogen , prothrombin fragment 1+2 , D dimer , plasminogen , plasmin antiplasmin complex and an increase in protein C activity , a decrease in antithrombin activity , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) , and a slight decrease in the sensitivity to activated protein C , but no significant change in that of the thrombin antithrombin complex . ^^^ In users of the DNG containing OCs , the reduction in total and free protein S , and in t PA and PAI was dependent on the EE dose , while factor 7 activity was elevated , but not significantly different from EE / LNG . ^^^ The effects of EE / EV / DNG on total and free protein S and on t PA and PAI were lower than those of 20EE / DNG , suggesting that the impact of 2 mg EV on several hemostasis variables is less than that of 10 microg EE . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In contrast , in PAI 1 stab substitution at either P 18 , P 19 or P 20 resulted in a u PA specificity and a significantly increased substrate behaviour towards t PA and u PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
BACKGROUND : The aim of this study was to evaluate the effect of the amlodipine atorvastatin combination on plasma tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) activity in hypercholesterolemic , hypertensive patients with insulin resistance . ^^^ At the end of the placebo wash out and of each treatment period , office BP , total cholesterol , PAI 1 , and t PA activity were evaluated . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Consequently , PAI 1 plays an important role in cardiovascular diseases ( mainly through inhibition of t PA ) and in cell migration and tumor development ( mainly through inhibition of u PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Red wine supplementation , in both diets , resulted in decreased plasma fibrinogen ( p=0 . 001 ) and factor VIIc ( p=0 . 05 ) , and in increased t PA ( p=0 . 01 ) and PAI 1 ( p=0 . 0003 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of a dietary intervention to reduce body weight on red cell aggregation ( RCA ) , factor 7 activity , plasminogen activator inhibitor ( PAI ) activity , tissue plasminogen activator ( t PA ) antigen , fibrinogen , whole blood and plasma viscosity , haematocrit and lipids . ^^^ MEASUREMENTS : Body weight and waist and hip circumferences , dietary intake by seven day weighed inventory , RCA , factor 7 activity , PAI activity , t PA antigen , fibrinogen , whole blood and plasma viscosity , haematocrit and lipids . ^^^ Factor 7 activity ( P = 0 . 0043 ) , RCA ( P = 0 . 01 ) and t PA antigen ( P = 0 . 016 ) were reduced in females after weight reduction but no differences were found in PAI activity , whole blood , plasma viscosity or haematocrit . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinogen , thrombocytes , factor 7 , factor 8 , von Willebrand factor ( vWF ) , thrombomodulin ( TM ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and other haemostatic factors , were described as more or less helpful in estimation of risk of occurrence of coronary artery disease and its cardiovascular complications . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fibrinogen , thrombocytes , factor 8 , von Willebrand factor ( vWF ) , thrombomodulin ( TM ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) and other haemostatic factors , play more or less essential role in unstable angina pectoris . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
We measured the ankle brachial index ( ABI ) and levels of D dimer , C reactive protein ( CRP ) , fibrinogen , serum amyloid A ( SAA ) , prothrombin 1 . 2 , t PA antigen , PAI 1 , and the t PA antigen / PAI 1 ratio . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Platelet count , fibrinogen , clotting times , prothrombin fragment ( PF ) 1+2 , and tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI ) activities were measured preoperatively , immediately before , and 5 min and 24 hr after aortic declamping . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Fasting serum lipids , homocysteine , total and free tissue factor pathway inhibitor ( TFPI ) , plasminogen activator inhibitor ( PAI 1 ) and tissue plasminogen activator ( t PA ) antigen and activity , C reactive protein ( CRP ) , interleukin 6 ( IL 6 ) and tumor necrosis factor alpha ( TNF alpha ) were measured . ^^^ Neither drug affected ( t PA ) antigen and activity , while fenofibrate increased PAI 1 antigen ( p < 0 . 05 ) and activity ( p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Therefore , the consequences of transverse aortic banding ( TAB ) were analyzed in mice lacking tissue type PA ( t PA ( / ) ) , urokinase type PA ( u PA ( / ) ) , or gelatinase B ( MMP 9 ( / ) ) , and in wild type ( WT ) mice after adenoviral gene transfer of the PA inhibitor PAI 1 or the MMP inhibitor TIMP 1 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Adopting the prospective , randomized controlled method to observe the coagulant fibrinolysis related indexes , including tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , von Willebrand factor ( vWF ) , antithrombin 2 ( AT 3 ) and fibrinogen ( FIB ) , in CHD patients after PCI , there were 26 patients in the treated group treated by TGC with Western medicine and 26 in the control group treated by Western medicine alone . ^^^ As comparison between the two groups , AT 3 and t PA were higher in the treated group than those in the control group ; PAI 1 levels showed insignificant difference at 1 month after treatment , but at 3 months after treatment , it was significantly lower in the treated group ( P < 0 . 05 ) ; vWF showed no significant difference either at 1 or at 3 months after treatment ; and FIB was lower in the treated group both at 1 and 3 months after treatment ( P < 0 . 01 ) . ^^^ CONCLUSION : TGC could improve the hypercoagulant status and adjust the balance between coagulant fibrinolysis system of CHD patients after PCI by increasing AT 3 and t PA levels , and lowering FIB and PAI 1 levels in the body . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were taken at baseline and up to 7 days after PCI and PAI 1 active antigen and tissue plasminogen activator ( t PA ) antigen were determined . ^^^ RESULTS : PCI increased both t PA and PAI 1 levels ( P < 0 . 001 ) , with a significant prolonged and pronounced increase in stent vs . balloon only patients ( P < 0 . 05 ) . ^^^ CONCLUSIONS : Coronary stent implantation significantly increases t PA and PAI 1 plasma levels up to 1 week compared with balloon angioplasty alone . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
All of the patients were treated with perindopril ( 4 mg / day ) and , after one and six months of therapy , their levels of plasma fibrinogen ( Fb ) , t PA antigen , PAI 1 antigen , von Willebrand factor ( vWF ) , ACE activity and blood pressure were measured . ^^^ Untreated patients had significantly higher levels of Fb , PAI 1 ( p < 0 . 01 ) and t PA ( p < 0 . 05 ) regardless of their ACE or PAI 1 genotypes . ^^^ Analysis of the group as a whole revealed an increase in t PA antigen levels after six months of treatment , regardless of ACE or PAI 1 genotype ( p < 0 . 01 ) . ^^^ It improved fibrinolysis by increasing t PA levels regardless of ACE and PAI 1 genotype . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
A . ) , plasminogen activity ( Berichrom Plasminogen Behring ) , tissue plasminogen activator antigen level ( t PA ) ( Imulyse t PA Biopool ) , plasminogen activator inhibitor 1 level ( PAI 1 ) ( Imulyse PAI 1 Biopool ) , plasmin alpha 2 antiplasmin complex level ( PAP ) ( Enzygnost PAP micro ) oraz D dimer level ( Vidas D Dimer bioMrieux S . ^^^ RESULTS : In children with chronic viral hepatitis statistically significant lower fibrinogen level ( p < 0 . 03 ) and PAI 1 level ( p < 0 . 0001 ) and higher t PA level ( p < 0 . 0001 ) in comparison to control group were found . ^^^ In children with chronic C hepatitis statistically significant lower PAI 1 level ( p < 0 . 0001 ) and fibrinogen level ( p < 0 . 005 ) and higher PAP level ( p < 0 . 0001 ) and t PA level ( p < 0 . 03 ) were found . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However , when treated with the anaphylotoxin C5a , mast cells express the PA inhibitor 1 ( PAI 1 ) in excess over t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Red wine supplementation in both diets , resulted in lower plasma fibrinogen ( p=0 . 001 ) and factor VIIc ( p=0 . 05 ) , and in increased t PA ( p=0 . 01 ) and PAI 1 ( p=0 . 0003 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The type and amount of dietary fat has been shown to alter thromboxane A 2 production and platelet aggregation , bleeding time , factor 7 , fibrinogen , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of plasminogen ( PLG ) , tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and D dimer were determined and compared . ^^^ RESULTS : The activity of t PA in NAFL patients was significantly lower than that in the control group ( P < 0 . 05 ) , and PLG and PAI 1 were significantly higher than those in the control group ( P < 0 . 05 ) . ^^^ In respect to the TCM Syndrome typing , in patients of PSS , t PA was significantly lower and PLG , PAI 1 were significantly higher than those in patients of non PSS ( P < 0 . 05 or P < 0 . 01 ) , while D dimer was insignificantly different between patients of the two Syndrome types ( P > 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There was a tendency for the prevalence of poorly controlled and masked hypertension to improve to the well controlled category in response to intensive therapy ( p=0 . 0625 , by Wilcoxon ' s signed rank test ) . 3 ) The plasma concentration of plasminogen activator inhibitor ( PAI 1 ) / tissue plasminogen activator ( t PA ) complex ( total PAI 1 complex ) was significantly decreased after intensive therapy ( 17 . 3+ / 7 . 8 ng / ml vs . 13 . 5+ / 4 . 6 ng / ml , n=28 , p < 0 . 01 by paired t test ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Additionally ; fibrinogen , plasminogen , plasminogen activator inhibitor ( PAI 1 ) , tissue plasminogen activator ( t PA ) and von Willebrand factor ( vWF ) levels were measured to determine the procoagulant activity . ^^^ Additionally ; procoagulant activity assessed by fibrinogen , plasminogen , PAI 1 , t PA and vWF levels was similar in both groups . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the blood plasma obtained in the couple of time points in the perioperative period the determinations were done as followed : the concentrations of von Willebrand factor antigen ( vWf Ag ) , tissue plasminogen activator antigen ( t PA Ag ) , antigen of plasminogen activator inhibitor type 1 ( PAI 1 Ag ) and fibrinogen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
CEE decreased the antigen levels of tissue plasminogen activator ( t PA ) from 8 . 8 to 6 . 8 U / ml ( p < 0 . 001 ) , and of plasminogen activator inhibitor ( PAI 1 ) from 30 . 8 to 21 . 6 U / ml ( p < 0 . 010 ) , whereas raloxifene had no significant effect on either t PA , from 9 . 6 to 9 . 2 U / ml ( p = 0 . 235 ) or PAI 1 antigen levels , from 32 . 1 to 30 . 4 U / ml ( p = 0 . 538 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
GCF levels of tissue type PA ( t PA ) , urokinase type PA ( u PA ) , PA inhibitor 1 ( PAI 1 ) and PA inhibitor 2 ( PAI 2 ) and serum concentrations of cotinine , u PA and PAI 1 were analysed by enzyme linked immunosorbent assay . ^^^ The ratio of u PA : PAI 1 and t PA : PAI 1 were significantly higher in GCF of smokers with periodontitis compared with `` healthy ' ' smokers , whereas the ratio of t PA : PAI 2 was significantly lower in smokers with periodontal disease ( p < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant change was observed in any group and under any treatment in regards to homocysteinemia , PAI 1 , or t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The 6 children with VOD ( 9 . 84 % ) had an early and significant increase in PAI 1 antigen and activity ( p < 0 . 0001 ) , t PA antigen ( p < 0 . 0001 ) and D dimer ( p < 0 . 01 ) levels , and a decrease in plasminogen , alpha 2 antiplasmin and PT emerged 2 ( + / 1 ) days before the clinical diagnosis of VOD by comparison with mean post HSCT values in the non VOD patients . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Troponin l , soluble P selectin , von Willebrand factor ( vWF ) , fibrinogen , plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) concentrations of 202 consecutive patients with non ST elevation acute coronary syndrome ( NSTE ACS ) were measured at the time of admission . ^^^ In these patients fibrinogen ( p < 0 . 001 ) , plasminogen ( p < 0 . 001 ) and PAI 1 ( p < 0 . 026 ) concentrations were higher and t PA concentrations were lower ( p < 0 . 013 ) and all these parameters correlated with P selectin concentrations ( p < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Lp ( a ) , fibrinogen , plasminogen , PAI 1 and t PA levels were measured and C reactive protein ( CRP ) assays were performed on admission for all patients , and venous blood samples were drawn 12 and 24 h later for cTn 1 measurements . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the main physiological inhibitor of tissue type ( t PA ) and urokinase type ( u PA ) plasminogen activator . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the main physiological inhibitor of tissue type ( t PA ) and urokinase type ( u PA ) plasminogen activator . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Increased plasminogen activator inhibitor 1 ( PAI 1 ) and decreased tissue plasminogen activator ( t PA ) activities lead to impaired fibrinolysis , which is critical for cardiovascular disease . ^^^ Plasma PAI 1 , t PA , and glucose as well as serum C peptide , insulin , total cholesterol , triglyceride , and HDL and LDL cholesterol levels were measured at 0 , 2 , 4 , and 6 h after the fat load . ^^^ The PAI 1 activities decreased significantly during the loading tests ( p < 0 . 0001 ) , whereas the t PA activities did not change . ^^^ We conclude that elevated PAI 1 and diminished t PA activities , suggestive of suppressed fibrinolysis , are present in our adolescents with type 2 diabetes ; adding another risk factor for cardiovascular disease and acute high fat load does not further negatively affect this suppressed fibrinolysis . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
AIM : The aim of this study was to observe the effect of a Chinese medicine compound Changtong oral liquid ( CT ) on tissue plasminogen activity ( t PA ) , plasminogen activator inhibitor ( PAI ) , TGF beta 1 and hydroxyproline ( OHP ) . ^^^ Forty New Zealand rabbits and 48 Sprague Dawley ( SD ) rats were assigned randomly to one of the five groups : sham adhesion , adhesion with saline , adhesion with low dosage of the CT , adhesion with middle dosage of the CT and adhesion with high dosage of the CT . t PA and PAI activity in plasma , OHP and TGF beta 1 expression in adhesion were investigated . ^^^ CONCLUSION : CT treatment significantly enhanced t PA activity in rabbits , but decreased TGF beta 1 content in rats , OHP content in cecum of rabbits , and failed to affect the activity of PAI and OHP content in abdominal wall in rabbits , compared with saline group . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No significant change was observed in any group and under any treatment in regards to homocysteinemia , PAI 1 , or t PA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Here , the effect of plasminogen activator inhibitor 1 ( PAI 1 ) , an endogenous inhibitor of t PA , on ischemic infarct volume was studied . ^^^ METHODS : After either permanent ligation or thrombotic occlusion of the middle cerebral artery ( MCA ) , infarct volume , spontaneous reperfusion of thrombosed MCA , t PA / PAI 1 complex level , and blood brain barrier ( BBB ) permeability in the ischemic region was studied in transgenic mice with overexpression of PAI 1 and wild type littermate controls and in mice with intracerebroventricular injection of human PAI 1 . ^^^ In the ligation model , t PA / PAI 1 complex levels were higher and BBB disruption was more pronounced in the ischemic region . ^^^ Human PAI 1 injection reduced infarct volume by about 50 % in wild type mice but not in t PA gene deficient mice . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We analyzed the impact of levothyroxine treatment on lipid parameters , fibrinogen , platelet count , D dimers , alpha 2 antiplasmin activity , plasminogen activity , tissue plasminogen activator antigen ( t PA Ag ) , plasminogen activator inhibitor type 1 antigen ( PAI 1 Ag ) and coagulation factors ( factor 8 coagulant , von Willebrand factor antigen , von Willebrand factor and factor 9 ) in 23 patients with severe hypothyroidism ( TSH level > 50 mU / 1 ) . ^^^ RESULTS : Mean fibrinogen levels increased by 14 . 2 % while t PA Ag and PAI 1 Ag increased by 42 . 6 and 69 % , respectively , after correction of hypothyroidism . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Before and after plateletpheresis , blood samples were taken and markers of fibrinolysis ( PAP , t PA , PAI 1 ) as well as factor 12 activity have been measured . ^^^ There were no significant changes in concentrations of t PA , PAI 1 and PAP as well as PAI 1 activity after plateletpheresis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tumor associated prognostic factors of the plasminogen activator family : determination and clinical value of u PA , t PA , PAI 1 , and PAI 2 ] . ^^^ Proteolytic factors belonging t the plasminogen activator family ( plasmin , u PA , t PA , u PAR , PAI 1 , and PAI 2 ) , which usually are involved in blood clotting and degradation of blood clots , are also present in healthy and diseased tissue of the kidney , lung , liver , gastro intestinal tract , breast , prostate , ovary , and brain . ^^^ Plasminogen activators u PA and t PA , their inhibitors PAI 1 and PAI 2 , and the u PA receptor ( u PAR , CD 87 ) are often elevated in solid malignant tumour tissues compared to their normal counterparts . ^^^ In breast cancer patients , an elevated tumour tissue extract antigen content of u PA , PAI 1 , and u PAR is associated with increased tumour aggressiveness and poor prognosis ; in contrary , an elevated content of t PA and PAI 2 indicates a favourable prognosis . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Seventeen men and 28 women , 50 75 years old , who were generally overweight to obese , were assessed for plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) activity , t PA antigen and plasma lipoprotein lipid levels , and body composition before and after 6 months of endurance exercise training while on a low fat diet . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In a multivariable regression model adjusting for t PA ( tissue plasminogen activator ) / PAI 1 ratio , SLEDAI , age at visit , family history of cardiovascular disease , SLE disease duration and body mass index , every 1 ng / mL increase in PAI 1 was associated with a reduction of 0 . 07 units FMD ( P = 0 . 039 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The aim of work was the evaluation of alterations within fibrinolysis system ( estimation of t PA and PAI 1 levels ) , fibrinogen concentration ( Fb ) and ACE activity with regard to co existent dyslipidemia and features of left ventricle hypertrophy ( LVH ) . ^^^ In a whole HT group significantly higher levels of PAI 1 , t PA and Fb were noted in comparison to NT group , considerably more pronounced within DLP rather than NLP subgroups . ^^^ It has been proved that 4G / 4G homozygous subjects of 4G / 5G PAI 1 gene polymorphism from HT group tend to present higher levels of PAI 1 and t PA if contrasted to 4G / 4G genotype of NT group , with more distinct effect within DLP subgroup . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
RESULTS : At the end of the red wine intake period , LDL / HDL , fibrinogen , factor 7 , plasma C reactive protein and oxidized LDL Ab were significantly decreased , while HDL C , Apo A 1 , TGFbeta 1 , t PA , PAI and total plasma antioxidant capacity were significantly increased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Prior to administration of thrombolytic therapy , venous blood was collected from 110 patients presenting with acute ST segment elevation myocardial infarction and plasma assayed for tissue plasminogen activator ( t PA ) antigen and activity , plasminogen activator inhibitor type 1 antigen ( PAI 1 ) , TAFI antigen and activity , C reactive protein ( CRP ) and sCD40L concentrations . ^^^ There were no significant associations between coronary reperfusion and plasma concentrations of t PA , PAI 1 , TAFI , CRP or sCD40L . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Experimental studies demonstrate a direct impact of viral components ( gp 120 , TAT ) on the endothelium , as they lead to expression of adhesion molecules ( intercellular adhesion molecule [ ICAM ] , E selectin ) , a prothrombotic state ( increase of von Willebrand factor , plasminogen activator inhibitor [ PAI ] 1 , tissue plasminogen activator [ t PA ] , tissue factor ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate urokinase plasminogen activator ( u PA ) , urokinase plasminogen activator soluble receptor ( su PAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) plasma levels in SSc patients ( pts ) versus healthy controls and their modulation by intravenous alphacyclodestrine ( Alprostadil ) . ^^^ METHODS : Plasma levels of u PA , su PAR , PAI 1 and t PA were measured in 40 SSc ( 34 lSSc and 6 dSSc ) pts and in 30 healthy controls . ^^^ Infusions of Alprostadil modulate u PA , su PAR , PAI 1 and t PA , restoring near normal levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
No difference between the groups was observed in plasma thrombus precursor protein , prothrombin fragment 1+2 , D dimer , t PA , PAI 1 , TM and thrombin activatable fibrinolysis inhibitor . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following measurements were taken : prothrombin index , prothrombin time , thrombin time , fibrinogen , factor 7 , ATIII , D dimers , INR , APTT , protein C and PAI 1 activity , t PA activity , PAI 1 antigen , t PA antigen , glucose level , insulin level , E 2 and total fat . ^^^ RESULTS : In the AN group the INR , prothrombin time , PAI 1 activity , t PA activity , protein C level were increased , whereas PAI 1 antigen , t PA antigen , prothrombin index , fasting insulin level and fasting glucose level were decreased . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In preterm preeclampsia , significantly reduced antithrombin 3 ( ATIII ) and PAI 2 levels with further elevated tissue type PA ( t PA ) antigen and plasminogen activator inhibitor 1 ( PAI 1 ) antigen were seen compared to normal pregnancy . ^^^ Neonates born to mothers of either normal pregnancy or preeclampsia at term showed similar hemostatic changes with reduced fibrinogen , ATIII , t PA , u PA antigen , PAI 1 levels , and coagulation activation compared to their respective maternal plasma levels . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
There were no significant differences in neither fasting levels or in circadian variation of t PA antigen , PAI 1 activity , PAI 1 antigen , factor 7 coagulant activity or fibrinogen between the three diets . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Serum CRP , plasma t PA and PAI 1 ( plasminogen activator inhibitor type 1 ) concentrations were determined in 95 patients with stable coronary heart disease . ^^^ Serum CRP concentrations correlated with plasma t PA concentrations ( r=0 . 37 , P < 0 . 001 ) and t PA / PAI 1 ratio ( r= 0 . 21 , P < 0 . 05 ) . ^^^ Intra arterial TNF alpha caused a rise in t PA concentrations ( P < 0 . 001 ) without affecting blood flow or PAI 1 concentrations . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Levels of t PA and PAI 2 in gingival crevicular fluid during orthodontic tooth movement in adults . ^^^ OBJECTIVE : The aim of this study was to investigate gingival crevicular fluid ( GCF ) levels of tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor ( PAI 2 ) during orthodontic tooth movement in adults . ^^^ GCF levels of t PA and PAI 2 were determined by commercially available ELISA kits . ^^^ RESULTS : After 24 hours of tooth movement the levels of t PA and PAI 2 in the GCF were significantly higher from the experimental canines compared with the control teeth . ^^^ CONCLUSIONS : These results indicate that the amounts of t PA and PAI 2 in the GCF increase with orthodontic tooth movement , and suggest that such increases may be involved in extracellular matrix degradation in response to mechanical stress . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Blood samples were obtained before t PA administration to measure fibrinogen , PAI 1 , Factors 7 and 10 . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasma global fibrinolytic capacity ( GFC ) , tissue plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) levels were measured . ^^^ Diabetic patients had lower GFC and t PA / PAI 1 levels than control subjects ( p < 0 . 05 , p < 0 . 05 ) . ^^^ After rosiglitazone treatment , there was no difference in mean plasma levels of GFC , t PA , PAI 1 and t PA / PAI 1 in diabetics . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The following coagulation tests were performed before and after maximal exercise : prothrombin time ( PT ) , partial activated thromboplastin time ( PTT ) , fibrinogen ( FBG ) , antithrombin 3 ( ATIII ) , protein C ( PC ) , protein S ( PS ) , prothrombin fragment 1+2 ( F1+2 ) , tissue activator of plasminogen ( t PA ) and its inhibitor ( PAI ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
As gene variants predisposing to thrombophilia and influencing the increased risk of vein thrombosis might influence response to local thrombolysis , the aim of the work was to characterize the pharmacogenetic conditions for local streptokinase treatment in patients with a deep vein thrombosis ( DVT ) of lower extremities based on the following polymorphism analyses : G1691A polymorphism of factor 5 ( FV ) , G20210A polymorphism of prothrombin ( PT ) , A4250G ( Thr312Ala ) polymorphism of fibrinogen alpha ( FGA ) , G ( 455 ) A polymorphism of fibrinogen beta ( FGB ) , 4G / 5G polymorphism of plasminogen activator inhibitor type 1 ( PAI 1 ) and insertion / deletion ( I / D ) polymorphism of tissue plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Prothrombin time ( PT ) , activated partial thromboplastin time ( aPTT ) , fibrinogen , factors 5 , 7 , 8 , 9 and 10 activities , vWF , antithrombin 3 ( AT 3 ) , protein C , protein S , tissue plasminogen activator ( t PA ) and tissue plasminogen activator inhibitor 1 ( PAI 1 ) , as well as common lipid variables , were measured . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) , von Willebrand factor ( vWF ) , and soluble thrombomodulin ( sTM ) are haemostatic markers of endothelial injury related to hypertension . ^^^ Haemostatic parameters ( t PA , PAI 1 , Pbetathromboglobulin ( PbetaTG ) , vWF , Fb ) and ACE activity were determined using ELISA , ELFA , chronometric and spectrophotometric methods . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Activities and concentrations of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and t PA / PAI complex were determined in all specimen by ELISA kits . ^^^ There were no differences between the groups regarding t PA concentrations , PAI 1 activity and t PA / PAI complex . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In the absence of TGF beta 1 , the expression of thrombomodulin , the plasminogen activators u PA and t PA , and their inhibitor PAI 1 was significantly increased in the S / G2 compared to the G 1 phase . ^^^ Treatment of endothelial cells with TGF beta 1 , however , resulted in elevated expression of PAI 1 specifically in the S / G2 phase , while t PA and u PA increased to the same extent in both the G 1 and S / G2 phase . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
However significant improvement of fibrinolysis via a decrease in PAI 1 activity level from 16 . 3 ( 3 . 7 ) to 14 . 8 ( 6 . 3 ) AU / ml ( p < 0 . 024 ) and an increase in t PA activity from 2 . 3 ( 0 . 8 ) to 2 . 7 ( 0 . 5 ) IU / ml and t PA antigen from 7 . 5 ( 2 . 9 ) to 9 . 2 ( 2 . 7 ) ng / ml ( p < 0 . 01 ) in experimental group were observed when compared between pre and post acute submaximum exercise ( 65 % VO ( 2 peak ) ) at the end of the program . ^^^ The results showed that no significant differences in tissue plasminogen activator levels ( t PA ) ( both antigen and activity ) , plasminogen activator inhibitor 1 levels ( PAl 1 ) ( both antigen and activity ) were observed in control and experimental groups after exercise training for 8 weeks as compared to the baseline . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Three months after delivery we investigated the plasma levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) , fibrinogen and von Willebrand factor ( vWF ) in 74 women with p GDM and 20 healthy females with normal glucose tolerance during and after pregnancy , as well as the relation of fibrinolytic parameters to insulin resistance and glycaemic control . ^^^ Mean PAI 1 and t PA as well as fasting and stimulated plasma concentrations of proinsulin , C peptide and insulin were elevated , whereas the disposition index was lower in women with p GDM and IIS compared with women with p GDM and NIS and the controls ( P < 0 . 001 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Patients with CHD were older , had a higher body mass index , and higher levels of total cholesterol , glucose , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( t PA ) antigen , and fibrinogen ; 47 % were treated for hypertension . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Studies provide that NO also participate in fibrynolysis regulation by the influence on the PAI 1 and t PA expression , what may have important clinical implications . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
METHODS : Including 21 patients , the following markers were measured preoperatively and on postoperative day 1 , 2 , 3 , 7 , 14 , and 21 : Activation products of coagulation : thrombin antithrombin complex ( TAT ) , thrombus precursor protein ( TPP ) , and prothrombin fragment F1+2 ( F1+2 ) , and markers of fibrinolysis : plasmin alpha ( 2 ) antiplasmin complexes ( PAP ) , D Dimer , tissue plasminogen activator ( t PA ) antigen , and plasminogen activator inhibitor 1 ( PAI 1 ) antigen . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Effect of troglitazone on serum gamma glutamyltransferase activity and plasma t PA / PAI 1 complex in type 2 diabetic patients . ^^^ Plasma tissue plasminogen activator ( t PA ) / plasminogen activator inhibitor 1 ( PAI 1 ) complex was decreased by treatment from 21 . 7 + 8 . 6 ng / ml to 19 . 0 + / 6 . 6 ng / ml ( F = 8 . 784 , p < 0 . 001 ) . ^^^ CONCLUSION : Decreases in serum gamma GT activity and plasma t PA / PAI 1 complex were closely related with antioxidant action of troglitazone . . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The plasminogen activator inhibitor ( PAI ) 1 , tissue plasminogen activator ( t PA ) and PAI I / t PA ratio were assessed before and after treatment . ^^^ RESULTS : Treatment with HCT spironolactone caused an increase in PAI 1 ( p < 0 . 001 ) and t PA ( p < 0 . 001 ) , while no changes were observed in PAI I / t PA ( P > 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
In 15 patients who had been treated with chronic haemodialysis , we measured and compared platelet aggregation ( induced by adenosine diphosphate , collagen and epinephrine ) , the markers of coagulation and fibrinolysis activation ( thrombin antithrombin complexes , thrombin fragments F1+2 , D dimer ) , and fibrinolytic parameters , i . e . fibrinogen , plasminogen , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 antigen and activity , before and immediately after the regular haemodialysis sessions . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in children with ulcerative colitis ] . ^^^ The aim of the study was the assessment of tissue plasminogen activator ( t PA ) and its inhibitor ( PAI 1 ) in children with ulcerative colitis . ^^^ The levels of t PA and PAI 1 were measured with ELISA . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) is an important component of the plasminogen / plasmin system as it is the main inhibitor of tissue type ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^ Consequently , PAI 1 plays an important role in cardiovascular diseases ( mainly through inhibition of t PA ) and in cell migration and tumor development ( mainly through inhibition of u PA and interaction with vitronectin ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The increased plasma t PA ag in persons with carotid stenosis was not associated with increased plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Subjects had tissue plasminogen activator ( t PA ) antigen levels and activity and plasminogen activator inhibitor 1 ( PAI 1 ) activity measured before and after 6 mo of endurance exercise training . ^^^ Subject ' s DNA was typed for the PAI 1 4 G / 5 G and t PA I / D variants . ^^^ Baseline PAI 1 activity , t PA activity , and t PA antigen levels were not different among PAI 1 or t PA genotype groups . ^^^ Overall , exercise training did not change PAI 1 activity ( 0 . 43 + / 0 . 81 IU / mL , p = NS ) , increased t PA activity ( 0 . 37 + / 0 . 16 IU / mL , p = 0 . 02 ) , and decreased t PA antigen levels ( 0 . 88 + / 0 . 20 ng / mL , p < 0 . 001 ) . ^^^ Although the differences in changes with training were not significant among genotype groups , significant t PA antigen level improvements were evident only in PAI 1 4 G allele carriers and significant t PA activity increases only in PAI 1 4 G homozygotes . t PA genotype affected the training induced t PA antigen level improvements ( p = 0 . 033 ) after covarying for gender and baseline t PA antigen levels , with the smallest and largest reductions in the D homozygotes and I / D heterozygotes , respectively . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
During the next 24 hours when the cells were cultured in standard medium synthesis of interleukin 6 ( IL 6 ) , tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were studied . ^^^ When changes in the PAI 1 / t PA ratio were calculated , a strong tendency for increase of that value was seen in cells pretreated with . 9 % NaCl or Earles salts solution but not with PDF . ^^^ However , in cases of Hanks solution , a significant increase in the PAI 1 / t PA ratio was observed ( +104 % , P < . 01 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
Notably , hearts from t PA null mice released much less NE upon EFS than their wild type ( WT ) controls ( i . e . , a 76 . 5 % decrease ; P < 0 . 01 ) , whereas hearts from plasminogen activator inhibitor 1 ( PAI 1 ) null mice released much more NE ( i . e . , a 275 % increase ; P < 0 . 05 ) . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
The gender specific role of polymorphisms from the fibrinolytic , renin angiotensin , and bradykinin systems in determining plasma t PA and PAI 1 levels . ^^^ Tissue plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) directly influence thrombus formation and degradation and thus risk for arterial thrombosis . ^^^ We report here results from a genetic analysis of plasma t PA and PAI 1 levels in a large population based sample from the PREVEND study in Groningen , the Netherlands ( n = 2 , 527 ) . ^^^ We found that males had higher levels of natural log transformed t PA , and PAI 1 ( P < 0 . 01 ) compared to females . ^^^ Furthermore , we found that age , body mass index , and waist to hip ratio were significant predictors of t PA and PAI 1 in both females and males , and that the regression relationships between these factors and plasma t PA and PAI 1 were dependent on gender . ^^^
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
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Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00750 and P05121	Pubmed	SVM Score :0.0
NA