| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| We proposed that a cell selective regulatory protein coordinately regulates the expression of three enzymes that are required for the biosynthesis of corticosteroids : cholesterol side chain cleavage enzyme , steroid 21 hydroxylase , and the aldosterone synthase isozyme of steroid 11 beta hydroxylase . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| We detected mRNAs for adrenodoxin , P450scc ( cholesterol side chain cleavage enzyme ) and P450c11 beta ( 11 beta hydroxylase ) but not for P450c17 ( 17 alpha hydroxylase / 17 , 20 lyase ) or P450c11AS ( aldosterone synthase ) in rat brains and cultures of rat glial cells . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| In the ZG , administration of ACTH for 9 days provoked an increase in the level of StAR mRNA ( 210 270 % ) and a decrease in the levels of 3betaHSD , cytochrome P 450 aldosterone synthase ( P450aldo ) , and AT 1 receptor mRNA ( by 40 % , 70 % , and 90 % , respectively ) , whereas the levels of P450scc and P450C21 mRNA did not differ significantly from the control values . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| These results suggest that testosterone inhibits the basal and ANG 2 and ACTH stimulated release of aldosterone , via inhibition of aldosterone synthase activity and cytochrome P 450 side chain cleavage ( P450scc ) activity , and ACTH stimulated cAMP accumulation in rat ZG cells . . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| The effects of aging on the activity of aldosterone synthase and the expression of cytochrome P 450 side chain cleavage enzyme ( P450scc ) in ZG cells were determined by thin layer chromatography and Western blot analysis , respectively . ^^^ The conversions of steroidogenic precursors to aldosterone and the activity of aldosterone synthase as well as the expression of P450scc in ZG cells were lower in the old group than in the young group . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| RNA was reverse transcribed and probed with specific primers for side chain cleavage enzyme ( CYP11A ) , 3beta hydroxysteroid dehydrogenase , aldosterone synthase ( CYP11B2 ) , 11beta hydroxylase ( CYP11B1 ) , steroidogenic factor 1 , and steroid acute regulatory protein . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| To examine the effects of LRH on steroidogenic capacity we used reporter constructs prepared with the 5 ' flanking region of steroidogenic acute regulatory protein ( StAR ) , cholesterol side chain cleavage ( CYP11A1 ) , 3beta hydroxysteroid dehydrogenase type 2 ( HSD3B2 ) , 17alpha hydroxylase , 17 , 20 lyase ( CYP 17 ) , 11beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , real time reverse transcription polymerase chain reaction ( RT RTPCR ) assays were developed that quantify mRNA levels for steroidogenic acute regulatory protein ( StAR ) , cholesterol side chain cleavage ( CYP11A ) , 3beta hydroxysteroid dehydrogenase types 1 and 2 ( HSD3B1 and HSD3B2 ) , 17alpha hydroxylase ( CYP 17 ) , 21 hydroxylase ( CYP 21 ) , 11beta hydroxylase ( CYP11B1 ) , aldosterone synthase ( CYP11B2 ) and aromatase ( CYP 19 ) . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Uniquely , aldosterone ' s production is regulated at two critical enzyme steps : ( 1 ) early in its biosynthetic pathway ( the conversion of cholesterol to pregnenolone cholesterol side chain cleavage enzyme ) and ( 2 ) late ( the conversion of corticosterone to aldosterone by aldosterone synthase ) . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| The interactions of CYP11B1 ( cytochrome P 45011beta ) , CYP11B2 ( cytochrome P 450aldo ) and CYP11A1 ( cytochrome P 450scc ) were investigated by cotransfection of their cDNA into COS 1 cells . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| CYP11A and CYP11B have an identical intron exon organization , and CYP 17 and CYP21B have similar gene structures , but the two pairs of genes encoding mitochondrial and microsomal P 450 enzymes resemble each other poorly . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Contribution of Ad4BP , a steroidogenic cell specific transcription factor , to regulation of the human CYP11A and bovine CYP11B genes through their distal promoters . ^^^ We analyzed the upstream regions of the human CYP11A and bovine CYP11B genes , and identified a distal promoter in each gene . ^^^ The distal promoters are located at 1 . 8 to 1 . 5 kb in the upstream region of the CYP11A gene and 1 . 5 to 1 . 1 kb in the upstream region of the CYP11B gene . ^^^ On structural analyses of the regions , we identified two Ad 4 sites and a cAMP responsive element in the distal promoter of CYP11A , and two Ad 4 sites and one NF IL 6 binding site in the distal promoter of CYP11B . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Activation of CYP11A and CYP11B gene promoters by the steroidogenic cell specific transcription factor , Ad4BP . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Adrenodoxin ( Adx ) , a [ 2Fe 2S ] vertebrate type ferredoxin , transfers electrons from the NADPH dependent flavoprotein Adx reductase ( AdR ) to mitochondrial cytochrome P 450 enzymes of the CYP11A and CYP11B families , which catalyze key reactions in steroid hormone biosynthesis . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Competitive reverse transcription polymerase chain reaction ( RT PCR ) showed that CYP11A ( P450scc ) mRNA regulating the first step of Aldo synthesis was significantly reduced from week 9 of age by amlodipine , and that CYP11B2 ( P450aldo ) mRNA regulating the last step of Aldo synthesis was potently suppressed from 9 weeks of age by TCV 116 . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Promoter analyses suggested that SIK repressed gene expressions not only of CYP11A and StAR but also of CYP11B1 , CYP11B2 and SIK itself . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Real time RT PCR showed that APAs produced higher levels of HSD3B2 , CYP 21 ( 21 hydroxylase ) , and CYP11B2 mRNA , whereas CPAs produced higher levels of CYP11A ( cholesterol side chain cleavage ) , CYP 17 ( 17alpha hydroxylase / 17 20 lyase ) , HSD3B2 , and CYP11B1 ( 11beta hydroxylase ) mRNA compared with normal adrenal . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Mesangial cells expressed the mRNA of the LDL receptor and steroidogenic enzymes , such as P450scc , 3beta hydroxysteroid dehydrogenase ( 3beta HSD ) , 21 hydroxylase and CYP11B2 . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| After the chemical treatments , changes in mRNA expression of 11 steroidogenic genes ( CYP11A , CYP11B1 , CYP11B2 , CYP 17 , CYP 19 , CYP 21 , 3beta HSD 1 , 3beta HSD 2 , 17beta HSD 1 , StAR and HMGR ) were quantified using molecular beacon based real time RT PCR . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| Activities of P450scc , P 45011 beta and P450aldo , and P450C21 and P 45017 alpha were assayed in a reconstituted enzyme system using 20 alpha hydroxycholesterol , DOC , and progesterone , respectively , and the substrate and the extracted products were analyzed by HPLC . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| There are two main regulatory sites of aldosterone biosynthesis , the early rate limiting step by the P450scc and the final steps by the P450aldo . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| The effects of the extracellular potassium concentration on the expression of four steroidogenic cytochromes P 450 ( CYP11B2 , CYP11B1 , CYP11A1 , and CYP21A1 ) in cultured rat adrenal zona glomerulosa cells were studied by Northern blot analysis . ^^^ In zona fasciculata cells , CYP11B2 expression was not inducible by potassium ; CYP11B1 , CYP11A1 , and CYP21A1 mRNA levels increased in response to ACTH , but not in response to a high potassium concentration . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| The effects of a calcium channel blocker ( nifedipine ) and a protein synthesis inhibitor ( cycloheximide ) on the induction of four steroidogenic cytochromes P 450 ( CYP11B2 , CYP11B1 , CYP11A1 , and CYP21A1 ) by an elevated extracellular potassium concentration were studied in cultured rat zona glomerulosa cells . ^^^ |
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| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19099 and P05108 |
Pubmed |
SVM Score :0.0 |
| NA |
|