| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| Studies in our laboratory have shown that baculovirus generated mac 25 binds IGF 1 and 2 in a specific manner , leading to the renaming of mac 25 as IGFBP 7 ( 3 ) . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| On the other hand , IGFBP rP 1 binds insulin with very high affinity ( 500 fold higher compared to other IGFBPs ) , but has low affinity for IGF 1 and 2 proteins ( k ( d ) = 3 10 10 ( 8 ) M ) . ^^^ |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| Insulin sensitivity ( insulin sensitivity index [ S ( 1 ) ] from intravenous glucose tolerance tests in nondiabetic subjects , or the rate constant for disappearance of glucose [ K ( ITT ) ] from insulin tolerance tests in type 2 diabetic subjects ) , circulating IGFBP rP 1 ( from enzyme linked immunosorbent assay ) , adiponectin ( from radioimmunoassay ) , C reactive protein ( CRP ; from immunoturbidimetry ) , soluble tumor necrosis factor receptor 2 ( sTNFR 2 ; from enzyme amplified sensitivity immunoassay ) , and IGF system parameters ( IGF 1 , free IGF 1 , and IGFBP 1 from immunoradiometric assay ) were assessed in all subjects . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| The inverse correlation between mac25 / IGFBP 7 expression and myogenic differentiation was further examined by treating myogenic cultures with transforming growth factor beta ( TGF beta ) or insulin like growth factor 1 ( IGF 1 ) . ^^^ IGF 1 stimulated differentiation by 50 % and inhibited mac25 / IGFBP 7 expression 2 to 3 fold . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBPs 1 6 bind IGF 1 and IGF 2 with high affinity whereas IGFBP 7 , a newly identified IGFBP , binds IGFs with lower affinity and constitutes a low affinity member of the IGFBP family . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP related protein 1 ( IGFBP rP 1 ) , the product of the mac 25 gene , binds IGF 1 , IGF 2 , and insulin , and we postulated that glucocorticoids regulate IGFBP rP 1 synthesis in osteoblasts . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP related protein 1 ( IGFBP RP 1 ) , the product of the mac 25 gene , binds IGF 1 , IGF 2 , and insulin . ^^^ GH , insulin , bone morphogenetic protein 2 , fibroblast growth factor 2 , platelet derived growth factor BB , IGF 1 , and IGF 2 did not modify mac25 / IGFBP RP 1 expression , whereas transforming growth factor beta 1 was modestly stimulatory . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| In marked contrast , AM precursor peripheral blood monocytes , do not express neuropeptides or IGF 1 and only express IGFBP 1 , 4 and 6 and IGFBP rP 1 . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| In random normal human adult sera ( n = 37 ) , the median IGFBP rP 1 was 21 . 0 micro g / liter , and values did not correlate with levels of IGF 1 ( r = 0 . 085 , P = 0 . 61 ) , IGF 2 ( r = 0 . 051 , P = 0 . 75 ) , or IGFBP 3 ( r = 0 . 061 , P = 0 . 74 ) . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| This is the first study that suggests inactivation of IGFBP rP 1 is through CpG methylation , and tumor suppressive activity of IGFBP rP 1 is through induction of apoptosis in an IGF 1 independent manner in prostate cancer . . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| Recombinant human mac 25 protein specifically binds IGF 1 and 2 . ^^^ Binding of 125I IGF 1 and 125I IGF 2 to rh mac 25 was demonstrated by Western ligand blotting after nondenaturing polyacrylamide gel electrophoresis and by affinity cross linking with as little as 2 nM rh mac 25 . ^^^ In comparison with IGFBP 3 , rh mac 25 has at least a 5 6 fold lower affinity for IGF 1 and 20 25 fold lower affinity for IGF 2 . mac 25 mRNA was detectable in a wide range of normal human tissues , with decreased expression in breast , prostate , colon , and lung cancer cell lines . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| Binding of insulin to IGFBP 3 and its N and C terminal fragments and of IGF 1 and IGF 2 to the structurally related proteins mac 25 and connective tissue growth factor was also investigated . ^^^ Weak insulin binding to full length IGFBP 3 could be demonstrated in a few experiments , but we found that binding of IGF 1 , IGF 2 , and insulin to mac 25 or connective tissue growth factor was below the detection limit of the biosensor instrument . . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| PSF activity is not due to insulin , insulin like growth factor 1 ( IGF 1 ) , growth hormone , glucocorticoids , or combinations of these hormones . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| Functional analysis of these mutants by transfection experiments in a cell line overexpressing the IGF 1 receptor ( NWTb 3 ) found that an inability to bind PSF significantly increased the IGFRE transcriptional activity , while retaining responsiveness to IGF 1 . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| An insulin like growth factor 1 ( IGF 1 ) response element ( IGFRE ) in the porcine P 450 cholesterol side chain cleavage gene ( P450scc ) binds two transcription factors , Sp 1 and polypyrimidine tract binding protein associated splicing factor ( PSF ) . ^^^ Western blot analysis showed a greater expression of PSF in Y 1 cells when compared with a mouse fibroblast cell line ( NWTb 3 ) in which IGF 1 stimulates the P450scc IGFRE . ^^^ Moreover , a mutant oligonucleotide IGFRE reporter construct that lacks PSF binding was responsive to IGF 1 . ^^^ In conclusion , Y 1 adrenal cells are a physiological example of PSF repression of growth factor stimulated ( IGF 1 ) gene expression ( P450scc ) . ^^^ |
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| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| PSF represses transcription of the insulin like growth factor 1 ( IGF 1 ) inducible gene P450scc by binding to an insulin like growth factor response element ( IGFRE ) motif in the gene . ^^^ |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16270 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|