Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.50724824
To begin to investigate the interaction between IGF 1 and IGFBP 5 in brain , we asked whether IGF 1 influences the brain expression of IGFBP 5 . 0.50724824^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.56219546
These data indicate that separate , specific regions of IGFBP 5 are responsible for interactive effects with IGF 1 as well as direct effects on MC activity . . 0.56219546^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.79581376
To delineate the interaction between IGF 1 and IGFBP 5 on wv granule neurons , we examined neuronal survival after treating with IGF 1 , des ( 1 3 ) IGF 1 , or IGFBP 5 antibody . 0.79581376^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The mRNAs for mechano growth factor , IGF binding protein 5 , and the IGF 1 receptor were unchanged by RE . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The protein was evident on 125I IGF 1 ligand blots and was immunostained with IGFBP 5 antibodies . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 mRNA was not affected by bFGF alone , but its enhancement by IGF 1 was attenuated by bFGF . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Like GH , IGF 1 normalized IGFBP 3 mRNA levels in liver , but , in contrast to GH , had no effect on IGFBP 5 mRNA in WAT . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 alone increased serum level of IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 but not IGFBP 3 stimulated thymidine incorporation into DNA both in the absence and presence of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The major cell associated binding site for [ 125I ] IGF 1 is IGFBP 3 and for [ 125I ] IGF 2 are IGFBP 3 and IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Large increases in serum levels of IGF 1 and IGFBP 5 and a transient increase in serum IGFBP 4 were found . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In contrast to the latter , IGFBP 5 ( 1 169 ) preferentially formed ternary complexes with IGF 2 rather than IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
At the protein level , IGF 1 clearly increased IGFBP 5 levels in conditioned medium . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These include reductions in growth hormone , IGF 1 , IGFBP 3 , and IGFBP 5 and an increase in IGFBP 4 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
RESULTS : IGF 1 , ICGF 2 , IGF 1R , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 , and IGFBP 6 were expressed by LAM cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 2 , and IGFBP 5 were present in equine seminal plasma . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 mRNA was increased in SMP 8 IGF 1 TG jejunum and ileum and was specifically upregulated in ileal lamina propria . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In normal testes a clear expression of IGF 1 , IGF 2 , IGF IR , IGFBP 2 , IGFBP 4 and IGFBP 5 was found . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
However , ES did not significantly alter the transcription of igf 1 , igfbp 2 , igfbp 5 and igfbp 6 genes . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Serum levels of IGF 1 , IGFBP 3 , and IGFBP 5 declined with age , while serum IGFBP 4 increased with age . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The levels of IGFBP 4 and IGFBP 5 were increased in MCF 7 cells by estradiol and IGF 1 , respectively , indicating that these BPs may contribute to the growth stimulatory response to these mitogens . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 and 2 caused a minimal ( less than 43 % ) increase in IGFBP 5 mRNA abundance and had no effect on IGFBP 4 mRNA abundance . ^^^ IGF 1 and 2 ( 100 ng / ml ) stimulated 6 8 fold increases in IGFBP 5 levels , whereas IGFBP 4 was inhibited . ^^^ Immunoblotting for IGFBP 5 revealed an M ( r ) 23 , 000 ( non IGF 1 binding ) fragment . ^^^ The amount of fragment formation was attenuated by the presence of IGF 1 and 2 , but not insulin , suggesting that this is a mechanism by which the IGFs act to modulate IGFBP 5 concentration . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Expression of IGF IA / KDEL in FRTL 5 cells , however , neither augmented TSH stimulated DNA synthesis nor stimulated IGF binding protein 5 expression , as does IGF IA expression in transfected FRTL 5 cells and the addition of exogenous IGF 1 to wild type FRTL 5 cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In this study we show that an IGF 1 analog that binds minimally to IGFBPs potently enhances the differentiation of the stringently controlled inducible C 2 myoblast ( C2l ) cell line and identify IGFBP 5 as the sole IGFBP secreted during C2l differentiation . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 , IGF 2 , and IGF binding protein 5 ( IGFBP 5 ) mRNAs were measured with a ribonuclease protection assay in the gastrocnemius of specific pathogen free Fischer 344 rats . ^^^ We hypothesized that IGF 1 , IGF 2 , and IGFBP 5 mRNA concentration ( normalized to 18S RNA ) in the gastrocnemius muscle of growing animals ( 3 mo ) would be downregulated in a coordinated manner with muscle size during aging associated atrophy . ^^^ We conclude that the aging associated atrophy of skeletal muscle is not caused by altered pretranslational regulation of IGF 1 , IGF 2 , or IGFBP 5 in skeletal muscle . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The present study shows that IGFBP 5 gene expression is also highly abundant during brain development and also demonstrates significant spatiotemporal correlation with IGF 1 , but exhibits a neuroanatomical distribution that is entirely distinct from IGFBP 2 . ^^^ IGFBP 5 and IGF 1 mRNAs are synchronously coexpressed in principal neurons of sensory relay systems , including the olfactory bulb , medial and dorsal lateral geniculate bodies , and ventral tier , cochlear , lemniscal , and vestibular nuclei . ^^^ They are also transiently coexpressed in principal neurons of the anterodorsal nucleus , but IGF 1 mRNA disappears from this structure shortly after birth , while IGFBP 5 mRNA remains highly abundant here in the adult . ^^^ IGFBP 5 and IGF 1 gene expression demonstrate a temporally coordinated laminar association in the developing cerebellar cortex and hippocampal formation . ^^^ IGF 1 mRNA is concentrated in Purkinje cells , while IGFBP 5 mRNA is localized in the external germinal zone in the developing cerebellar cortex . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF binding protein 5 and IGF 1 receptor regulation in hypophysectomized rat kidneys . ^^^ Ligand blotting with IGF 1 demonstrated a 31 kDa protein in both membranes , identified by immunostaining as IGF binding protein 5 ( IGFBP 5 ) , not IGFBP 1 or IGFBP 2 . ^^^ Adaptations in the kidney after HYPX include an upregulation of the IGF 1 receptor as well as IGFBP 5 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The concentrations of both IGFBPs were increased by the addition of IGF 1 , IGF 2 , or insulin to the cell cultures , with IGFBP 5 demonstrating the greatest hormonal stimulation . ^^^ The effects of IGF 1 on IGFBP 5 expression were both time and dose dependent , with IGF 1 being more potent than IGF 2 , and IGF 2 more potent than insulin . ^^^ Northern blotting demonstrated that IGF 1 increased the expression of the mRNA for IGFBP 5 , whereas dexamethasone decreased it . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment of these MDGCs with IGF 1 dramatically stimulated the production ( to a detectable level ) of a 30 kDa IGFBP that was identified by immunoblotting with antiserum to IGFBP 5 but not antisera to IGFBP 1 , 2 , 3 , 4 , or 6 . ^^^ IGFBP 5 mRNA in these cultures was correspondingly stimulated by IGF 1 but unaffected by FSH . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Signaling by insulin like growth factors in paralyzed skeletal muscle : rapid induction of IGF 1 expression in muscle fibers and prevention of interstitial cell proliferation by IGF BP 5 and IGF BP 4 . ^^^ To determine whether IGFs may participate in the initiation of restorative reactions in inactivated muscle we analyzed the expression of IGF 1 and IGF 2 mRNA in botulinum toxin paralyzed and in denervated rat skeletal muscle , and interfered with the activity of IGFs by locally applying the IGF binding proteins IGF BP 5 and IGF BP 4 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In this study we have exploited the high affinity and specificity of IGF binding protein 4 ( IGF BP 4 ) and IGF BP 5 for IGF 1 and IGF 2 to determine whether these growth factors are involved in the nerve sprouting reaction in paralyzed skeletal muscle . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment with IGF 1 ( 100 ng / ml ) resulted in a substantial ( P < 0 . 05 ) increase in the accumulation of IGFBP 5 , the major 28 29 kDa IGFBP species . ^^^ Des ( 1 3 ) IGF 1 , a type 1 receptor selective ligand with markedly reduced avidity for IGFBPs , proved substantially more potent ( as a promoter of IGFBP 5 accumulation ) than its native counterpart . ^^^ In contrast , treatment with IGF 2 or [ Leu 27 ] IGF 2 , type 2 IGF receptor selective ligands , yielded a more limited effect on IGFBP 5 accumulation in keeping with an overall rank order of potency of des ( 1 3 ) IGF 1 > IGF 1 > IGF 2 > or = [ Leu 27 ] IGF II . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ IGF 1 stimulates granulosa cell derived insulin like growth factor binding protein 5 : evidence for medication via type 1 IGF receptors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 and IGF 2 had no effect on IGFBP 2 steady state transcript levels but enhanced the level of IGFBP 5 transcripts ( approximately fourfold ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Heparin exposure was associated with a 17 fold decrease in the affinity of IGFBP 5 for IGF 1 . ^^^ It did not directly compete with IGF 1 for binding to IGFBP 5 , suggesting that heparin binding to this region of IGFBP 5 resulted in a conformational change in IGFBP 5 which lowered its affinity for IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
This effect was most pronounced for IGFBP 5 and was also elicited by an IGF 1 analog that retains affinity for IGFBPs but not by insulin or IGF analogs that have decreased affinity for IGFBPs . ^^^ Additionally , this effect was not associated with a change in IGFBP 5 messenger RNA ( mRNA ) levels ; however , the appearance of IGFBP 5 in the conditioned medium was inhibited by an anti IGF 1 receptor antibody ( alpha IR 3 ) . ^^^ RA decreased IGFBP 5 mRNA levels and cell associated IGFBP 5 in both the presence and absence of IGF 1 and inhibited the IGF 1 stimulated secretion of IGFBP 5 into T47D cell conditioned medium . ^^^ These results suggest that IGF 1 increases IGFBP 5 levels in the T47D cell line both through direct interaction with IGFBP 5 as well as through a receptor mediated process that does not require direct interaction with IGFBPs . ^^^ Thus , IGFBP 5 accumulation appears to be positively regulated by IGF 1 , potentially at the level of susceptibility to proteolysis , and negatively regulated at the level of gene expression by RA . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We have previously reported the presence of proteolytic activity in conditioned medium from human fibroblast cultures that cleaves insulin like growth factor binding protein 5 ( IGFBP 5 ) into non IGF 1 binding fragments . ^^^ Coincubation of IGF 1 or IGF 2 and IGFBP 5 with fibroblast cultures decreased proteolysis . ^^^ The purified protease cleaved IGFBP 5 into 22 , 20 , and 17 kilodalton non IGF 1 binding fragments . ^^^ This suggests that the IGF 1 induced increase in IGFBP 5 in fibroblast medium is only partially due to direct protease inhibition . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These findings support the view that the IGF system plays a fundamental role in endometrial biology , acting via autocrine and / or paracrine mechanisms , with IGF 1 and IGFBP 5 being dominant in the proliferative phase , and IGF 2 and the other IGFBPs predominant in the secretory phase of the menstrual cycle . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These findings suggest that loss of IGFBP 5 in SV 40 transformed fibroblasts facilitates interaction of endogenously produced IGF 1 with the IGF 1 receptor and increases their sensitivity to autocrine stimulation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
CONCLUSIONS : Experimental colitis in rats causes an increase in IGF 1 binding to the muscularis propria , which represents increased levels of IGFBP 4 and IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In bovine fibroblasts , IGF 1 treatment induced IGFBP 3 and IGFBP 5 mRNA and protein secretion , and had a moderate effect on IGFBP 4 expression . ^^^ IGF 1 also increased steady state levels of IGFBP 5 mRNA . ^^^ Dexamethasone alone decreased IGFBP 3 , IGFBP 4 , and IGFBP 5 mRNA , but it had no significant effect on IGFBP 3 , 4 , or 5 expression in the presence of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 3 was stimulated by IGF 1 , IGFBP 4 by forskolin , and IGFBP 5 by IGF 1 . ^^^ IGFBP 2 , 3 , and 4 are expressed under basal conditions whereas IGFBP 5 is only detectable after IGF 1 induction . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To better understand the role of insulin related growth factors in neural development , we have characterized by in situ hybridization in chicken embryonic retina the patterns of gene expression for insulin , insulin like growth factor 1 ( IGF 1 ) , their respective receptors and the IGF binding protein 5 ( IGFBP 5 ) from early stages ( E 6 ) until late stages ( E 18 ) an analysis not performed yet in any species . ^^^ These findings suggest a near constitutive expression of insulin receptor and IGF 1 receptor genes , while IGF 1 and IGFBP 5 showed a highly focal spatiotemporal regulation of gene expression . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment of Ob cells with either IGF 1 or all trans retinoic acid ( RA ) caused a time and dose dependent increase in IGFBP 5 messenger RNA ( mRNA ) levels , as determined by Northern blot analysis . ^^^ Stimulation of IGFBP 5 mRNA was obtained at 100 nM IGF 1 between 6 and 16 h ( 2 to 2 . 5 fold ) and 100 nM RA between 16 and 24 h ( 3 to 4 fold ) . ^^^ Concomitant treatment of Ob cells with IGF 1 and RA revealed an additive effect and a 5 to 7 fold increase in IGFBP 5 mRNA levels after 16 24 h . ^^^ The effect of IGF 1 and RA and their combination on IGFBP 5 transcripts was similar in confluent and subconfluent cultures of Ob cells . ^^^ IGF 1 and RA did not change IGFBP 5 mRNA stability in Ob cells after transcription arrest with the RNA polymerase 2 inhibitor 5 , 6 dichloro 1 beta D ribofuranosyl benzimidazole . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 and IGF 2 treatment elevated IGFBP 5 in conditioned media , but longR3IGF 1 and insulin , which do not bind to IGFBPs , had smaller effects . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In contrast to IGFBP 4 , IGFBP 5 treatment increased the binding of IGF tracer to bone cells but did not increase the binding of 125I IGF 1 to type 1 IGF receptor . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In this study , we describe the mRNA expression of IGF 1 , IGF 2 , type 1 IGF receptor , IGFBP 2 , IGFBP 4 and IGFBP 5 during mouse lung development as studied by in situ hybridization techniques . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The validity of the IGFBP 5 RIA was established from the following data : 1 ) recombinant human IGFBP 5 and purified human bone derived IGFBP 5 inhibited the binding of tracer to the antisera in an identical manner ; 2 ) the immunoreactive IGFBP 5 in serum co migrated with recombinant human IGFBP 5 in both sodium dodecyl sulfate polyacrylamide gel electrophoresis and high performance gel filtration chromatography ; 3 ) none of the other known IGFBPs ( IGFBP 1 , 2 , 3 , 4 , and 6 ) exhibited significant cross reactivity ; 4 ) the addition of IGF 1 or IGF 2 to the samples did not affect the recovery of IGFBP 5 ; and 5 ) the recovery of the exogenously added IGFBP 5 to serum was greater than 90 % . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 , type 1 IGF receptor ( IGFR 1 ) , IGFBP 2 and IGFBP 5 mRNA are localized in distinct cell populations , and all are expressed at the highest levels at the peak of Purkinje cell growth , active synaptogenesis and dendritic formation . ^^^ The altered IGFBP 5 gene expression in granule cell precursors may modulate the interaction of IGF 1 with IGFR 1 in ways that contribute to their massive death occurring in the development of wv / wv cerebellum . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Conditioned media IGFBP 5 increased in response to IGF 1 , IGF 2 , and insulin in a dose dependent fashion , compared with unstimulated FRTL 5 cells . ^^^ The addition of a monoclonal antibody ( Sm 1 . 2 ) to IGF 1 completely inhibited IGF 1 stimulation of the IGFBP 5 6 kilobase transcript and the appearance of IGFBP 5 in FRTL 5 conditioned media . ^^^ TSH , on the other hand , inhibited basal levels of IGFBP 5 mRNA and attenuated the increase in IGFBP 5 mRNA stimulated by IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Since extracellular matrix ( ECM ) has been shown to be a reservoir for IGF 1 and IGF 2 , we examined the ECM of cultured human fetal fibroblasts and found that IGFBP 5 was incorporated intact into ECM , while mostly inert proteolytic fragments were found in the medium . ^^^ ECM associated IGFBP 5 had a sevenfold decrease in affinity for IGF 1 compared to IGFBP 5 in solution . ^^^ Furthermore , when IGFBP 5 was present in cell culture substrata , it potentiated the growth stimulatory effects of IGF 1 on fibroblasts . ^^^ When IGFBP 5 was present only in the medium , it was degraded to a 22 kD fragment and had no effect on IGF 1 stimulated growth . ^^^ We conclude that IGFBP 5 is present in fibroblast ECM , where it is protected from degradation and can potentiate the biologic actions of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 increased the levels of IGFBP 2 and IGFBP 5 in conditioned media by greater than tenfold but had no effect on IGFBP 2 and IGFBP 5 mRNA levels , again suggesting the involvement of posttranscriptional controls . ^^^ Pretreatment of MDA MB 468 and MDA MB 231 cells with IGF 1 receptor antibody ( alpha IR 3 ) blocked the IGF 1 effect on IGFBP 3 levels in the media in both cell lines and IGFBP 2 and IGFBP 5 secreted levels in MDA MB 468 cell conditioned media . ^^^ The addition of RA also blocked IGF 1 stimulation of IGFBP 2 and IGFBP 5 levels . ^^^ Cycloheximide treatment completely blocked the RA and / or IGF 1 mediated modulation of these binding proteins , suggesting that these agents enhance IGFBP 3 , IGFBP 2 , and IGFBP 5 synthesis and consequent secretion . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment of U 2 cells with IGF 1 markedly increased medium levels of IGFBP 5 in a concentration and time dependent manner ; other skeletal regulatory factors ( GH , insulin , PTH , dexamethasone , beta estradiol , 1 , 25 dihydroxyvitamin D 3 , transforming growth factor beta ) had no effect . ^^^ IGF 1 increased IGFBP 5 levels in the culture medium 10 fold without influencing IGFBP 5 messenger RNA abundance . ^^^ IGF 1 , IGF 2 , and the IGF 1 analog [ 1 27Gly ( 4 ) 38 70 ] IGF 1 bound IGFBP 5 with high affinity and , when added to U 2 cultures , effectively promoted IGFBP 5 accumulation in the medium . ^^^ On the other hand , des ( 1 3 ) IGF 1 and [ QAYL ] IGF 1 , IGF 1 analogs that did not bind IGFBP 5 , failed to elicit an increase in medium IGFBP 5 . ^^^ Cell free incubation of recombinant human ( rh ) IGFBP 5 in U 2 conditioned medium resulted in a marked reduction of detectable rhIGFBP 5 ; the presence of IGF 1 or IGF 2 peptide partially prevented this decrease . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In addition , a monoclonal antibody against IGF 1 ( Sm 1 . 2 ) inhibited the PTH induced increase in medium IGFBP 5 without influencing IGFBP 5 transcript levels . ^^^ Direct addition of IGF 1 to UMR cell cultures increased medium IGFBP 5 levels approximately 14 fold , with a modest effect on IGFBP 5 mRNA levels . ^^^ Studies comparing IGF 1 , IGF 2 , different IGF 1 analogs , and insulin indicated that the predominant IGF effect on IGFBP 5 accumulation was type 1 IGF receptor independent . ^^^ Thus , in UMR 106 01 cells , PTH and IGF 1 increase extracellular concentrations of IGFBP 5 via distinct but coordinate mechanisms ; PTH acts primarily to induce IGFBP 5 mRNA expression through a cAMP mediated mechanism , and IGF 1 appears to interact directly with IGFBP 5 protein to promote its accumulation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
When GC conditioned medium ( GC CM ) was assessed by Western Ligand Blotting ( WLB ) , FSH appeared to decrease IGFBP 5 , whereas IGF 1 appeared to increase IGFBP 5 and to partially block the effects of FSH treatment . ^^^ The ability of FSH treated GC CM to proteolyze IGFBP 5 was reduced by the addition of IGF 1 to the reaction mixture . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Recently we demonstrated that a 23 kDa form of IGFBP 5 , derived from osteoblast like cells , stimulates osteoblast mitogenesis and enhances IGF 1 action . ^^^ Because osteoblast derived IGFBP 5 is smaller than recombinant intact IGFBP 5 ( 23 vs 30 kDa ) and has decreased binding affinity for IGF 1 , we proposed that the native 23 kDa form of IGFBP 5 was truncated at a carboxy terminal position . ^^^ We now show that a recombinant form of carboxy truncated IGFBP 5 binds IGF 1 with reduced affinity and stimulates mitogenesis in mouse osteoblasts . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Interestingly , attachment of IGF 1 or 2 to IGFBP 4 results in enhancement of proteolysis , whereas attachment of either growth factor to IGFBP 5 results in inhibition of proteolytic cleavage . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 affinity labeling studies demonstrated three proteins in both glomerular membranes and proximal tubule BLM ; molecular weight approximately 140 , 000 d , the alpha subunit of the IGF 1 receptor , a protein > 200 , 000 d , and a protein approximately 31 , 000 d that was immunostained with IGF binding protein 5 antibodies . ^^^ These studies suggest that cytosolic hypertrophy of proximal nephron structures is accompanied by membrane hypertrophy with a fixed density of IGF 1 receptor and IGF binding protein 5 . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 mRNA and IGFBP 5 mRNA are localized in both adipocytes and stromal vascular cells , whereas IGFBP 2 and 3 expression is restricted to stromal vascular tissue . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Because of the physiologic importance of IGF 1 and its potential therapeutic properties , the renal sites of mRNA synthesis for IGF 1 , IGF IR , and IGFBP 1 through IGFBP 5 were characterized in rat kidney by in situ hybridization . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
After treatment with parathyroid hormone ( PTH , 10 ( 8 ) M ) or prostaglandin E 2 ( PGE 2 , 10 ( 6 ) M ) , immunoreactive IGFBP 5 accumulated in the conditioned medium in a 21 kDa form which did not bind IGF 1 on Western ligand blots . ^^^ Addition of IGF 1 at 10 ( 8 ) M into the culture resulted in accumulation of native 31 kDa IGFBP 5 . ^^^ However , even in the presence of IGF 1 , the native IGFBP 5 was degraded and the 21 kDa product accumulated in the culture medium . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Schwann cells ( SCs ) were the main source of IGF 1 and IGFBP 5 immunoreactivity until 7 days after nerve transection , when invading macrophages in the distal nerve stumps were strongly IGF 1 positive . ^^^ Northern analysis revealed that SCs expressed IGF 1 receptor and IGFBP 5 . ^^^ IGF 1 treatment increased the intensity of IGFBP 5 without affecting gene expression . ^^^ Incubation of recombinant human IGFBP 5 with SC conditioned media revealed IGF 1 protection of IGFBP 5 from proteolysis , implying the presence of an IGFBP 5 protease in SC conditioned media . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Coordinate IGF 1 and IGFBP 5 gene expression in perinatal rat brain after hypoxia ischemia . ^^^ To understand the regulation of the action of IGF 1 in response to such a metabolic insult , we investigated the gene expression of IGF 1 , type 1 IGF receptor , IGF binding protein ( IGFBP ) 2 , and IGFBP 5 during the first 72 h after hypoxia ischemia in the immature rat . ^^^ At 72 h of recovery , although IGFBP 2 and type 1 IGF receptor mRNA levels remain suppressed , gene expression of both IGF 1 and IGFBP 5 was activated in reactive astrocytes . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
During regeneration following folic acid induced acute renal failure , IGF 1 , IGFBP 3 , and IGFBP 5 mRNAs declined in abundance approximately two to threefold . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In addition , prolonged , but not short term , stimulation with EGF resulted in autophosphorylation of the IGF 1 receptor , and coincubations with both EGF and IGFBP 5 attenuated this effect . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Heparin binding to insulin like growth factor ( IGF ) binding protein 5 ( IGFBP 5 ) leads to a 17 fold decrease in its affinity for IGF 1 , and a region that contains several basic amino acids ( Arg 201 Arg218 ) may be involved in this affinity shift . ^^^ Their association constants ( Ka ) for IGF 1 were similar to native IGFBP 5 . ^^^ Affinity cross linking studies showed that heparin was equipotent in inhibiting the formation of 125I IGF 1 K134A / Rl36A mutant complexes compared to native IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) regulates IGF binding protein 5 synthesis through transcriptional activation of the gene in aortic smooth muscle cells . ^^^ In this cell type , the biosynthesis of IGFBP 5 is up regulated by IGF 1 . ^^^ The induction of IGFBP 5 mRNA by IGF 1 is time and dose dependent and requires de novo protein synthesis . ^^^ The IGF 1 induced IGFBP 5 gene expression is cell type specific because IGF 1 had no such effect in other cell types examined . ^^^ Nuclear run on assays revealed that IGF 1 increased transcription rate of the IGFBP 5 gene , while IGF 1 did not change the IGFBP 5 mRNA stability . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 , the most conserved of six IGFBPs characterized to date , uniquely potentiates the anabolic actions of IGF 1 for skeletal cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The inhibition of myogenic differentiation by high level expression of IGFBP 5 could be overcome by exogenous IGFs , with des ( 1 3 ) IGF 1 , an analogue with decreased affinity for IGFBP 5 but normal affinity for the IGF 1 receptor , showing the highest potency . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment of cells with IGF 1 and IGF 2 resulted in a dose dependent increase of IGFBP 5 and a small increase in IGFBP 4 in conditioned media ( CM ) . ^^^ Des ( 1 3 ) IGF 1 and [ Gln 6 , Ala 7 , Tyr 18 , Leu 19 ] IGF 2 ( [ QAYL ] IGF 2 ) , which bind to the type 1 IGF receptor but not to IGFBPs , also induced IGFBP 5 peptide , although the increase was less than with IGF 1 or IGF 2 treatment of RAC . [ Leu 27 ] IGF 2 , which does not bind to the type 1 IGF receptor but binds to IGFBPs , resulted in little induction of IGFBP 5 , while [ QAYL Leu 27 ] IGF 2 , which has reduced affinity for both the type 1 IGF receptor and IGFBPs , did not increase IGFBP 5 . ^^^ Northern blotting analysis showed that IGF 1 , IGF 2 and des ( 1 3 ) IGF 1 treatment of RAC increased steady state levels of IGFBP 5 mRNA , suggesting that the IGF mediated increase in IGFBP 5 is transcriptionally modulated . ^^^ IGFBP 5 protease activity was detectable in untreated CM , whereas treatment with IGF 1 and IGF 2 partially protected IGFBP 5 from proteolysis . ^^^ In summary , treatment of RAC with IGF 1 and IGF 2 results in dose dependent increases in both IGFBP 5 peptide in the CM and mRNA levels . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Previous studies have characterized IGFBP 5 and IGF 1 gene expression in the developing nervous system . ^^^ The current results , coupled with the known profile of IGF 1 expression during nervous system development demonstrates the colocalization of IGF 1 and IGFBP 5 in PNS , cerebellum , and brain stem . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In osteoarthritic chondrocytes , IGF 1 message was increased 3 . 5 fold , IGFBP 3 was increased 24 fold , and IGFBP 5 was increased 16 fold over normal chondrocytes . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Northern blots showed readily detectable transcripts for IGF 1 ( 6 . 7 and 0 . 9 kb ) , IGFBP 2 ( 1 . 8 kb ) , IGFBP 3 ( 2 . 8 kb ) , IGFBP 4 ( 2 . 6 kb ) , and IGFBP 5 ( 6 . 0 kb ) , but not for IGFBP 6 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 increases IGFBP 5 mRNA levels in both cell types , whereas retinoic acid stimulates IGFBP 5 mRNA expression in calvaria but suppresses it in Saos 2 cells . ^^^ IGFBP 5 mRNA expression is stimulated in normal bone cells by parathyroid hormone . 30 nM IGFBP 5 stimulates 3H thymidine incorporation in calvaria ( which produce IGF 1 contributing to basal proliferation in serum free medium ) but not in Saos 2 cells which produce little IGF 1 and IGF 2 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Mutation of the lysine 211 resulted in no change in the affinity of IGFBP 5 for ECM or heparin Sepharose ; however , it resulted in a major reduction in affinity for IGF 1 following heparin binding . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In subjects from 23 87 years , serum IGFBP 4 concentrations showed a significant positive correlation with serum PTH while serum IGFBP 5 concentrations showed a significant positive correlation with IGF 1 . ^^^ These age related changes in the serum levels of IGF system components are consistent with our previous findings of age related decreases in the femoral cortical contents of IGF 1 , IGF 2 and IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We also examined the effect of IGFs on IGFBP 5 protease activity , and found that IGF 1 and 2 inhibited the proteolysis in cell free conditioned medium , while des ( 1 3 ) IGF 1 was less effective . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The prospective training study was associated with 1 ) increases in circulating osteocalcin ( 39 + / 14 % ; P < 0 . 007 ) , and 2 ) decreases in IGF 1 ( 14 + / 5 % ; P < 0 . 05 ) and IGFBP 5 ( 10 + / 4 % ; P < 0 . 04 ) . ^^^ In fact , training may , in the short term , have led to a catabolic state hormonally expressed by reductions in IGF 1 and IGFBP 5 . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To investigate the contribution of the insulin like growth factors ( IGFs ) and their binding proteins ( IGFBPs ) to the regulation of bone growth in 10 GH deficient Japanese children receiving recombinant GH therapy , we determined the percent increase from pretreatment levels of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , and bone specific alkaline phosphatase isoenzyme ( B ALP ) . ^^^ The elevation of IGF 1 induced by GH was significantly correlated with the increases in IGFBP 3 ( r = 0 . 735 ; P < 0 . 0001 ) and IGFBP 5 ( r = 0 . 795 ; P < 0 . 0001 ) , and the elevation of B ALP was also significantly positively correlated with the increases in IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 ( r = 0 . 544 , P < 0 . 0001 ; r = 0 . 268 , P = 0 . 0399 ; r = 0 . 414 , P = 0 . 0010 ; and r = 0 . 500 , P < 0 . 0001 , respectively ) . ^^^ Our data are consistent with the anabolic effect on bone growth of GH treatment being mediated by IGF 2 , IGFBP 3 , and IGFBP 5 as well as by IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
GHRs identified by chemical crosslinking of 125I hGH to cell monolayers increased after treatment with IGFBP 5 and decreased in response to insulin like growth factor 1 ( IGF 1 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 receptor , and five IGF binding proteins ( IGFBPs ) , IGFBP 1 , IGFBP 2 , IGFBP 3 , IGFBP 4 , and IGFBP 5 in smooth muscle cells ( SMCs ) using colloidal gold immunocytochemistry . ^^^ Quantitative reverse transcription polymerase chain reaction ( QRT PCR ) performed on de novo atherectomy specimens identified mRNA for IGF 1 , IGF 1 receptor , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 levels and detected mRNA for IGFBP 3 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 , and IGFBP 4 and 5 mRNAs showed site specific tubular changes whilst remaining unchanged in other parts of the kidney normally expressing the genes : IGF 1 and IGFBP 4 mRNAs were reduced in TALs and proximal tubules respectively ; IGFBP 5 mRNA was reduced in most distal tubular cells but strongly expressed in a few of these cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
CONCLUSION : These results indicate a coordinate gene expression of the IGF system in microembolised porcine myocardium , compatible with a role of IGF 1 , IGFBP 3 , IGFBP 5 , and IGFBP 6 in inflammation linked angiogenesis and / or repair processes . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 5 in Schwann cell differentiation . ^^^ Cultured SCs also express IGF binding protein 5 ( IGFBP 5 ) , which can modulate the actions of IGF 1 . ^^^ The concomitant up regulation of IGFBP 5 may play a role in targeting IGF 1 to SCs and thus increase local IGF 1 bioavailability . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Later in the wounding response ( 7 14 dpl ) , the expression of IGFBP 4 and IGFBP 5 peaked within astrocytes and neurons , with IGFBP 5 mRNA being specifically localized to the glia limitans within the wound , suggesting an inhibitory role for these proteins , down regulating the effects of IGF 1 chronically . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Dibutyryl cAMP , forskolin , and hPTH ( 1 34 ) significantly stimulated mRNA expression of both IGF 1 and IGF binding protein 5 ( IGFBP 5 ) in these cultures , but neither 10 ( 7 ) M PMA , a PKC activator , nor 10 ( 7 ) M A 23187 did . ^^^ Moreover , anti IGF 1 antibody significantly blocked osteoclastlike cell formation stimulated by the conditioned medium from MC3T3 E 1 cells pretreated with 10 ( 8 ) PTH ( 1 34 ) , which induced IGF 1 and IGFBP 5 mRNA expression in these cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Concomitant with a decrease in IGFBP 3 and IGFBP 5 release , La3+ caused an increase in [ 125I ] IGF 1 binding to cell associated IGFBPs and type 1 IGF receptors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In muscle GH receptor and IGF 1 gene expression , IGF 1 peptide and IGF binding protein 5 ( IGFBP ) levels were decreased . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 alone did not change the steady state mRNA levels of IGFBP 3 , 4 , and 6 , but elevated that of IGFBP 5 . ^^^ Exogenous IGFBP 5 inhibited the OP 1 induced alkaline phosphatase activity and reduced the synergistic stimulatory effect of IGF 1 and OP 1 . ^^^ These findings strongly suggest that the OP 1 induced down regulation of IGFBPs , especially that of IGFBP 5 , is an important mechanism by which OP 1 and IGF 1 synergize to stimulate FRC cell differentiation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 induces collagen and IGFBP 5 mRNA in rat intestinal smooth muscle . ^^^ IGF 1 ( 100 ng / ml ) increased IGFBP 5 mRNA ( 1 . 9 + / 0 . 1 fold ) and collagen type alpha 1 ( 1 ) mRNA ( 1 . 6 + / 0 . 2 fold ) in cultured smooth muscle cells . ^^^ IGF 1 induced a dose and time dependent increase in IGFBP 5 in conditioned medium by Western ligand blot and by immunoblot . ^^^ IGF 1 did not affect the IGFBP 5 mRNA decay rate after transcriptional blockade . ^^^ IGF 1 increases IGFBP 5 and collagen mRNAs in intestinal smooth muscle cells . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Arg 138 Arg139 > Asn 138 Asn139 mutations were introduced to create protease resistant IGFBP 5 , which has the same affinity for IGF 1 as the native protein . ^^^ This mutant IGFBP 5 remained intact even after 24 h of incubation and it inhibited several IGF 1 actions when added to pSMC culture medium . ^^^ The mutant IGFBP 5 ( 500 ng / ml ) decreased IGF 1 stimulated cellular DNA synthesis by 84 % , protein synthesis by 77 % , and it inhibited IGF 1 stimulated migration of pSMC by 77 % . ^^^ In contrast , the same amount of native IGFBP 5 did not inhibit IGF 1 actions . ^^^ The mutant IGFBP 5 accumulated in culture medium of transfected cells , while native IGFBP 5 was degraded into fragments , PSMC overexpressing the mutant IGFBP 5 also responded poorly to IGF 1 compared with mock transfected cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Synthetic IGFBP 5 ( 201 218 ) peptide increased the binding of IGFBP 5 to HA as well as the binding of IGF 1 to HA bound IGFBP 5 . ^^^ IGFBP 5 ( 201 218 ) was found to bind directly to IGFBP 5 and cause a threefold enhancement of the IGF 1 binding affinity for IGFBP 5 , whether IGFBP 5 was bound to HA or was in a matrix free fluid phase . ^^^ Heparin inhibited the binding of IGFBP 5 to HA and blocked the interaction of IGFBP 5 with IGFBP 5 ( 201 218 ) in the fluid phase , suggesting that the primary heparin binding domain of IGFBP 5 specifically enhances the binding of IGFBP 5 to HA and increases IGF 1 binding to IGFBP 5 . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The large increase in IGFBP 5 concentrations in milk from involuting mammary glands was inhibited by 90 % if the dams received concurrent PRL injections for 2 days , but was unaffected by GH , progesterone , corticosterone , or an antiserum to insulin like growth factor 1 ( IGF 1 ) . ^^^ We , therefore , propose that IGFBP 5 serves to inhibit IGF 1 mediated cell survival , but that it is normally suppressed by PRL and milk removal . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 stimulated jejunal IGFBP 5 mRNA , but GH had no effect on IGFBP 5 mRNA . ^^^ These results suggest that the ability of exogenous IGF 1 , but not GH , to induce IGFBP 5 mRNA in jejunal mucosa may lead to the selective growth promoting effect of IGF 1 . ^^^ We postulate that IGFBP 5 positively modulates the anabolic effects induced by exogenous IGF 1 in the jejunum . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 induced IGFBP 3 and IGFBP 5 proteins in a time dependent manner without an increase in the corresponding mRNAs . ^^^ IGF 1 and TGF beta differentially regulate IGFBP 3 , IGFBP 4 , and IGFBP 5 expression , which , in turn , may modulate the in vitro and in vivo action of IGF I . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Because the insulin like growth factor ( IGF ) autocrine paracrine system may provide an important signaling mechanism between TM cells and the outflow pathway , the expression of IGFBP 5 and IGF 1 receptor in the TM was characterized . ^^^ Western immunoblots and confocal immunohistochemistry were used to evaluate the protein expression patterns of IGFBP 5 and the IGF 1 receptor . ^^^ CONCLUSIONS : IGFBP 5 and IGF 1 receptor were expressed at significant levels by TM cells and may serve an important role in trabecular function . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
GH also increased the expression of both IGF 1 and IGF binding protein 5 mRNA as well as the release of IGF 1 from these cells . ^^^ The addition of IGF 1 or recombinant IGFBP 5 alone significantly increased ALP activity and type 1 procollagen mRNA expression . ^^^ These findings indicate that GH acts directly on osteoblasts to stimulate bone formation and that IGF 1 and IGFBP 5 are involved in GH stimulated bone formation . ^^^ In conclusion , GH stimulates osteoclastic bone resorption as well as osteoblastic bone formation in vitro , and locally produced IGF 1 and / or IGFBP 5 are involved in the stimulation of bone remodelling by GH . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Administration of estradiol to ovariectomized rats increases uterine weight and uterine IGF 1 gene expression , while immunostaining of IGFBP 5 in the luminal epithelial cells and longidinal smooth muscle cells is almost extinguished . ^^^ In vitro studies using primary uterine cells reveal that IGF 1 increases thymidine incorporation and inhibits IGFBP 5 gene expression , whereas estradiol has no effect suggesting that in vivo estradiol induced IGFBP 5 suppression is mediated through increased IGF 1 production . ^^^ Our results suggest that in vivo , the uterotrophic effects of estradiol are mediated at least in part by estradiol stimulated uterine IGF 1 expression which in turn inhibits IGFBP 5 expression , an action which would be expected to increase IGF bioactivity . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We have previously shown that secretion of IGFBP 4 and IGFBP 5 by L 6 and BC3H 1 muscle cells was stimulated by treatment with either insulin , IGF 1 , or IGF 2 . ^^^ IGFBP 5 secretion by BC3H 1 cells was increased by either insulin or IGF 1 . ^^^ IGFBP secretion by L 6 cells is stimulated by both insulin / IGF 1 and cAMP dependent pathways , whereas IGFBP 5 secretion by BC3H 1 cells is stimulated only by the insulin / IGF pathway . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To investigate the role of IGF 1 in the mechanisms of FSH stimulated inhibin alpha subunit ( Inh alpha ) production , rat GC were cultured with FSH , IGF 1 , insulin like growth factor binding protein ( IGFBP ) 4 , IGFBP 5 , and / or anti IGF 1 antibody . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Osteogenic protein 1 regulates insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 5 ( IGFBP 5 ) gene expression in fetal rat calvaria cells by different mechanisms . ^^^ Previous studies from our laboratory revealed that OP 1 led to a two to threefold increase in steady state insulin like growth factor 1 ( IGF 1 ) and IGF 2 mRNA levels and a fivefold decrease in IGF binding protein 5 ( IGFBP 5 ) mRNA levels in primary cultures of fetal rat calvaria ( FRC ) cells . ^^^ In conclusion , OP 1 regulates IGF 1 gene expression at the posttranscriptional level , but regulates IGF 2 and IGFBP 5 gene expression at the transcriptional level . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The ability of FSH to induce IGFBP 5 endopeptidase activity proved relatively specific in that other granulosa cell agonists such as activin A , IGF 1 , GnRH , interleukin 1beta , TNF alpha , TGF beta 1 , EGF , or endothelin 1 failed to do so . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We now demonstrate using three independent methods that human IGFBP 5 , when occupied by IGF 1 or IGF 2 , forms ternary complexes of approximately 130 kDa with the acid labile subunit . ^^^ As observed for IGFBP 3 , ternary complexes containing IGFBP 5 form preferentially in the presence of IGF 1 , even though IGFBP 5 has a preferential affinity for IGF 2 over IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Human alpha thrombin ( 0 . 0008 NIH U / ml ) cleaved IGFBP 5 into 24 , 23 , and 20 kDa non IGF 1 binding fragments . ^^^ The binding of IGF 1 to IGFBP 5 partially inhibited IGFBP 5 degradation by thrombin , and an IGF analog that does not bind to IGFBP 5 had no effect . ^^^ To determine the physiological significance of IGFBP 5 cleavage , the effect of an IGFBP 5 mutant that is resistant to cleavage by the pSMC protease and has been shown to inhibit IGF 1 actions in pSMC was determined . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Competitive binding studies , using either [ 125I ] IGF 1 or [ 125I ] IGF 2 , indicated that La3+ and Cr3+ did not affect [ 125I ] IGF 1 or [ 125I ] IGF 2 binding to cell associated IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The aim of this study was to determine if cultured porcine vascular smooth muscle cells ( pSMCs ) that had been maintained on different extracellular matrix proteins had an alteration in their expression of insulin like growth factor binding protein 5 ( IGFBP 5 ) and their responsiveness to insulin like growth factor 1 ( IGF 1 ) . ^^^ IGF 1 increased IGFBP 5 gene expression 3 fold in the cells plated on fibronectin . ^^^ The addition of IGFBP 5 to cultures plated on laminin and type 4 collagen significantly increased their response to IGF 1 . ^^^ These results indicate that fibronectin , laminin , and type 4 collagen have major effects on IGFBP 5 expression and on IGF 1 stimulated pSMC responses and that these effects are mediated by their respective integrins . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
CONCLUSIONS : These results suggest that the action of IGF binding protein 5 in CIS cells may modulate the activity of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Exposure of the cells to IGF 1 for 48 h resulted in the appearance of IGFBP 5 in the medium . ^^^ Des ( 1 3 ) IGF 1 , an IGF 1 analog with reduced affinity for IGFBPs , also increased the level of IGFBP 5 , but to a lesser extent than IGF 1 , whereas LR3IGF 1 , which has virtually no affinity for IGFBPs , had no effect on IGFBP 5 . ^^^ The degradation of IGFBP 5 was significantly inhibited by IGF 1 , but not by des ( 1 3 ) IGF 1 or LR3IGF 1 . ^^^ Furthermore , IL 1alpha , but not IGF 1 , induced IGFBP 5 messenger RNA expression , as assessed by Northern blot analysis . ^^^ Coincubation of IGF 1 with IL 1alpha resulted in a substantially increased IGFBP 5 protein level , suggesting a synergism between the mechanisms of action of these two factors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The mean serum IGFBP 5 level increased by about 2 fold within 3 months after the initiation of GH therapy and was correlated with serum IGF 1 ( r = 0 . 719 , 0 . 801 , and 0 . 722 before and after 18 and 36 months , respectively , p < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Previously , we have reported that porcine VSMCs synthesize and secrete IGF 1 and several forms of IGFBPs , including IGFBP 2 , IGFBP 4 , and IGFBP 5 . ^^^ In this study , we examined the role of autocrine / paracrine secreted IGF 1 in controlling the expression of IGFBP 4 and IGFBP 5 as well as the effects of these IGFBPs in modulating the cellular replication response to IGF 1 . ^^^ This culture density dependent change in IGFBP 5 mRNA correlated closely with endogenous IGF 1 levels . ^^^ Since treatment of VSMC with exogenous IGF 1 increased IGFBP 5 mRNA levels , we neutralized the effect of endogenously secreted IGF 1 with an anti IGF 1 antibody to determine if it would alter IGFBP 5 mRNA abundance . ^^^ IGFBP 5 , on the other hand , potentiated the effect of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Recently , we demonstrated that IGF 1 induces IGF binding protein 5 ( IGFBP 5 ) expression in cultures of osteoblast enriched cells from 22 day fetal rat calvariae ( Ob cells ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Extracellular matrix binding of IGFBP 5 leads to a decrease in its affinity for insulin like growth factor 1 ( IGF 1 ) , which allows IGF 1 to better equilibrate with IGF receptors . ^^^ When the amount of IGFBP 5 that is bound to ECM is increased by exogenous addition , IGF 1 ' s effect on fibroblast growth is enhanced . ^^^ Smooth muscle cells that constitutively express the mutants that bind weakly to ECM are less responsive to IGF 1 , suggesting that ECM binding of IGFBP 5 is an important variable that determines cellular responsiveness . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
When added in the presence of 30 ng / ml IGF 1 , IGFBP 5 ( 250 ng / ml ) inhibited all markers of the early proliferative response : the tyrosine phosphorylation of the cytoplasmic signaling molecules IRS 1 and Shc , the activation of the MAP kinases , ERK 1 and 2 , the elevation of c fos mRNA , the early inhibition of the elevation in myogenin mRNA , and the increase in cell number . ^^^ In contrast , IGFBP 5 stimulated all aspects of the myogenic response to IGF 1 : the later rise in myogenin mRNA , the elevation of creatine kinase activity , and the fusion of myoblasts into myotubes . ^^^ This dual response to IGFBP 5 was greatest when it was added at a molar ratio of IGFBP 5 to IGF 1 of 2 : 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The injured sciatic nerve expressed IGF 1 and IGF 2 as well as IGFBP 4 and IGFBP 5 , whereas central nervous system ( CNS ) scar tissue expressed IGF 1 , IGFBP 2 , and IGFBP 5 . ^^^ In line with the in vivo findings , cultured Schwann cells expressed IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 5 mRNAs , whereas cultured astrocytes expressed IGF 1 , IGFBP 2 , and IGFBP 5 mRNAs . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Whereas 6 6 5 , like IGFBP 6 , had a marked preference for binary complex formation with IGF 2 rather than IGF 1 , it formed ternary complexes more efficiently with IGF 1 , like IGF BP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To address this question , we performed a cross sectional study measuring serum levels of IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , IGFBP 4 and IGFBP 5 by specific immunoassays in 52 adults with Type 1 ( n=27 ) and Type 2 ( n=25 ) diabetes mellitus and 100 age and sex matched healthy blood donors . ^^^ IGF 1 correlated positively with IGFBP 3 and IGFBP 5 and negatively with IGFBP 1 and IGFBP 4 in all subjects . ^^^ Furthermore , biochemical markers indicating bone loss ( ICTP ) and increased bone turnover ( PTH , OSC ) correlated positively with IGFBP 1 and IGFBP 4 but negatively with IGF 1 , IGFBP 3 and IGFBP 5 , while the opposite was observed with bone formation markers ( PICP , B ALP ) and vitamin D 3 metabolites . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Structure of the IGF binding domain of the insulin like growth factor binding protein 5 ( IGFBP 5 ) : implications for IGF and IGF 1 receptor interactions . ^^^ Remarkably , the IGF 1 receptor binding assays of IGFBP 5 showed that IGFBP 5 inhibits the binding of IGFs to the IGF 1 receptor , resulting in reduction of receptor stimulation and autophosphorylation . ^^^ Compared with the full length IGFBP 5 , the smaller N terminal fragments were less efficient inhibitors of the IGF 1 receptor binding of IGFs . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Inhibition of IGFBP 5 binding to extracellular matrix and IGF 1 stimulated DNA synthesis by a peptide fragment of IGFBP 5 . ^^^ IGFBP 5 synthesis is stimulated five to sixfold by IGF 1 , and IGFBP 5 has been shown to augment IGF 1 stimulated DNA synthesis in this cell type . ^^^ The ability of IGFBP 5 to augment the SMC response to IGF 1 is dependent upon its binding to extracellular matrix . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
This study represents the most comprehensive and detailed analysis of the physiology and anatomy of the mouse ovarian IGF system , and shows that 1 ) IGFBP 5 is linked to the survival of the slow growing and immature preantral follicles ; 2 ) IGF 1 is associated with the growth and survival of the rapidly growing large preantral and antral follicles ; 3 ) IGFBP 4 is an atretogenic candidate for mouse ovarian follicles ; 4 ) ovulatory doses of PMSG / hCG up regulate IGFBP 2 mRNA expression in the ovarian interstitium ; and 5 ) transcripts of IGF type 1 receptor and IGFBP 2 through 5 , but not those of IGF 1 are highly expressed in the mouse corpus luteum . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Furthermore , insulin like growth factor 1 ( IGF 1 ) but not its structural analogues with reduced affinity for IGFBP 5 , was also able to partially protect IGFBP 5 from degradation indicating that the association of IGF with the binding protein was required for the inhibition of the proteolytic activity . ^^^ In conclusion , we show that IGFBP 5 is specifically cleaved by a serine protease released by primary cultures of ovine articular chondrocytes and also demonstrate the ability of IGF 1 to inhibit the proteolytic activity both in cell culture and in cell free conditions . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 3 inhibited and IGFBP 5 augmented IGF 1 induced proliferation . ^^^ We conclude that the expression and time dependent production of IGFBP 3 , IGFBP 4 , and IGFBP 5 and their regulation by endogenous TGF beta 1 represent mechanisms by which human intestinal muscle cells regulate autocrine IGF 1 mediated growth . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Cryostat sections of colon from TNB treated and control rats were hybridized with 35S labeled antisense probes for IGF 1 , IGFBP 3 , IGFBP 4 and IGFBP 5 . ^^^ Altered expression of IGFBP 3 , IGFBP 4 and IGFBP 5 in specific bowel layers during colitis suggests that they play a role in modulating IGF 1 action . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Identification of IGFBP 5 peptides in the fractions of our peptide bank generated from hemofiltrate was performed by their immunoreactivity and their capacity to bind IGF 1 . ^^^ Fractions containing C terminal IGFBP 5 fragments revealed significant IGF 1 binding properties . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 5 , and total protein were determined in extracts obtained after ethylenediamine tetraacetate and guanidine hydrochloride extraction . ^^^ Cortical bone contents of IGF 1 , IGF 2 , and IGFBP 5 were significantly elevated in the acromegalic patients compared with control values [ 91 % ( P < 0 . 001 ) , 44 % ( P < 0 . 04 ) , and 115 % ( P < 0 . 004 ) , respectively ] . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Growth of the slow growing clone , C6BP2 1 , could not be overcome by the addition of exogenous IGF 1 , suggesting that the cell associated IGFBP 5 was the dominant regulator of IGF action . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 protein in tumor extracts bound 125I labeled IGF 1 in ligand blot assays and the amounts of IGFBP 5 measured by immunoblotting paralleled the levels of IGFBP 5 mRNA . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The difference between IGF 1 and RA was an enhanced IGFBP 5 binding to the extracellular matrix ( ECM ) by RA , whereas IGF 1 reduced binding to the ECM . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Here we describe two highly conserved amino acids in the C terminal domain of rat IGFBP 5 that are involved in binding IGF 1 . ^^^ This shows that Gly 203 and Gln 209 in IGFBP 5 are important determinants in binding IGF 1 , and due to their complete conservation in all IGFBP sequences , we suggest that they are likely to be involved in binding IGF 1 in all six binding proteins . ^^^ In addition , these two non basic residues lie within the ECM binding region ( 201 218 ) of IGFBP 5 , demonstrating that the C terminus contains partially overlapping IGF 1 and ECM binding sites . ^^^ We therefore propose that heparin binding to basic amino acids in IGFBP 5 between 201 218 may physically occlude subsequent interaction between IGF 1 and Gly203 / Gln209 , and that this may explain previous work of others showing reduced affinity of ECM bound IGFBP 5 for IGF I . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We have previously demonstrated that VDRCs antagonize the growth promoting activity of IGF 1 by stimulating autocrine production of IGFBP 5 in MCF 7 cells , but the effect of VDRCs on IGF 1 receptor ( IGF IR ) intracellular signaling has not been elucidated . ^^^ Furthermore , we demonstrate that an antisense IGFBP 5 oligodeoxynucleotide attenuates EB 1089 induced inhibition of IGF 1 stimulated tyrosine phosphorylation of IRS 1 and EB 1089 induced IGFBP 5 accumulation . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To determine how cell death and changes in IGF 1 gene expression relate to the extent of hypoxia ischemia , we evaluated the time course of apoptosis in a neonatal hypoxia ischemia model in relation to the cellular distribution of IGF 1 and IGFBP 5 mRNA . ^^^ Severe hypoxia ischemia results in an immediate decrease in neuronal IGF 1 and IGFBP 5 mRNA . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 promotes SC survival and protects IGFBP 5 in SC conditioned medium from proteolysis . ^^^ In the current study we examined the roles of IGF 1 and IGFBP 5 in primary SC . ^^^ IGF 1 enhances primary SC differentiation and gene and protein expression of IGFBP 5 and the myelinating protein , P 0 . ^^^ The phosphatidylinositol 3 kinase inhibitor ( LY 294002 ) , but not the mitogen activated protein kinase kinase inhibitor ( PD 98059 ) , blocks IGF 1 enhancement of IGFBP 5 gene and protein expression . ^^^ Collectively , these results suggest that IGF 1 promotes SC differentiation , and this may occur in part by enhancing IGFBP 5 expression via phosphatidylinositol 3 kinase activation . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 stimulates the synthesis of IGF binding protein 5 by these cells . ^^^ Preincubation with the four most active compounds also resulted in significant inhibition of the ability of IGF 1 to stimulate IGF binding protein 5 synthesis . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Down regulation or inhibition of PKC activity abolished the IGF 1 induced DNA synthesis , migration and IGFBP 5 gene expression . ^^^ Because the actions of IGF 1 on DNA synthesis and IGFBP 5 gene expression in VSMCs have been shown to be mediated through the phosphatidylinositol 3 kinase ( PI 3 kinase ) signaling pathway in porcine VSMCs , the potential role of PKC in IGF 1 induced activation of PI 3 kinase and PKB / Akt were examined . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
A quantitative PCR measurement of messenger RNA expression of IGF 1 , IGF 2 and IGFBP 5 in human skeletal muscle . ^^^ We determined the presence of IGF 1 , 2 , and binding protein 5 ( IGFBP 5 ) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin . ^^^ IGF 1 , IGF 2 and IGFBP 5 mRNA are present in adult human skeletal muscle , but no significant changes between meal groups were observed for IGF 1 , IGF 2 or IGFBP 5 mRNA levels , indicating that the expression of these genes are not altered acutely by nutrients and insulin . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Serum IGF 1 ( 25 microg / l ) , IGF 2 ( 194 microg / l ) , IGFBP 3 ( 0 . 4 mg / l ) , and IGFBP 5 ( 87 microg / l ) levels were low compared to age matched prepubertal children . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 has been suggested to induce apoptosis by sequestering IGF 1 to casein micelles , thereby inhibiting its survival function . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The genes for IGF 1 and 2 , type 1 IGF receptor , IGFBP 2 , and IGFBP 5 are expressed in both granulosa and theca cells of the chicken ovary . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Regulation of IGF 1 , IGFBP 4 and IGFBP 5 gene expression by loading in mouse skeletal muscle . ^^^ Gene expression of IGF 1 , IGFBP 4 and IGFBP 5 was studied in hindhimb skeletal muscle of mice , which were either overloaded or unloaded for 8 days . ^^^ Unloading by hindlimb suspension resulted in atrophy of soleus muscle ( 20 % ) and phenotype change towards the fast type associated with a transient decrease of IGF 1 mRNA ( 30 % ) and a sustained increase ( x 2 ) of IGFBP 5 transcript . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 production by GT 1 7 cells was stimulated by both IGF 1 and IGF 2 in a dose dependent manner with approximately equal potency , whereas insulin caused no significant effect . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To determine whether IGF 1 action was altered , IGF binding protein 5 , which is synthesized in response to IGF 1 , was analyzed . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Expression of the insulin like growth factor binding protein 5 ( IGFBP 5 ) gene in vascular smooth muscle cells is up regulated by IGF 1 through an IGF 1 receptor mediated mechanism . ^^^ In this study , we studied the possible involvement of the mitogen activated protein kinase ( MAPK ) and PI 3 kinase signaling pathways in mediating IGF 1 regulated IGFBP 5 gene expression . ^^^ Inhibition of the MAPK activation by PD 98059 , however , did not affect IGF 1 stimulated IGFBP 5 expression . ^^^ When LY 294002 and wortmannin , two specific inhibitors of PI 3 kinase , were added with Des ( 1 3 ) IGF 1 , the IGF 1 regulated IGFBP 5 expression was negated . ^^^ The addition of rapamycin , which inhibits IGF 1 induced p 70 ( s6k ) activation , significantly inhibited IGF 1 regulated IGFBP 5 gene expression . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Down regulation of the serum stimulatory components of the insulin like growth factor ( IGF ) system ( IGF 1 , IGF 2 , IGF binding protein [ BP ] 3 , and IGFBP 5 ) in age related ( type 2 ) femoral neck osteoporosis . ^^^ Circulating concentrations of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 , IGFBP 5 , 25 hydroxycholecalciferol ( 25 ( OH ) D 3 ) , and intact parathyroid hormone ( PTH ) were measured in 50 elderly women after sustaining a hip fracture and in 50 healthy age matched controls . ^^^ No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^ In pooled data from the normal and osteoporotic populations , age adjusted multiple regression models based on IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 were found to be highly predictive of serum OC ( R 2 = 19 % , p < 0 . 001 ) and BMD of femoral neck ( R 2 = 49 % , p < 0 . 0001 ) , consistent with an effect of the anabolic IGF components on overall bone formation rate . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To test the hypothesis that impairment in bone formation in renal osteodystrophy in adults with chronic renal failure ( CRF ) might be mediated in part by alterations in circulating levels of the insulin like growth factor ( IGF ) system components , we compared serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 and IGFBP 5 in adults with CRF ( CRF patients with parathyroid hormone ( PTH ) < 100 pg / ml , PTH > 300 pg / ml and end stage renal failure ( ESRF ) patients ) versus age matched controls . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
When the smooth muscle cell growth response to these components was assessed , IGF 1 plus IGFBP 5 or the combination of TSP 1 or OPN with IGF 1 potentiated the IGF 1 effect . ^^^ These interactions may be important for concentrating intact IGFBP 5 in extracellular matrix and for modulating the cooperative interaction between the IGF 1 receptor and integrin receptor signaling pathways . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In all subjects , free and total IGF 1 levels showed significant negative correlations with IGFBP 1 , IGFBP 2 , and IGFBP 4 ( that is , inhibitory IGF system components ) and significant positive correlations with IGFBP 3 and IGFBP 5 ( that is , stimulatory IGF system components ) . ^^^ Histologic parameters of bone formation showed significant positive correlations with serum levels of IGF 1 , IGF 2 , and IGFBP 5 . ^^^ CONCLUSION : Patients with primary and secondary hyperparathyroidism showed lower levels of the putative stimulatory IGFBP 5 but higher levels of IGFBP 1 , 2 , 3 , and 6 , whereas total IGF 1 and IGF 2 levels were not or only moderately increased . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Substitutions for residues 68 , 69 , 70 , 73 , and 74 in IGFBP 5 ( changing one charged residue , Lys ( 68 ) , to a neutral one and the four hydrophobic residues to nonhydrophobic residues ) resulted in an approximately 1000 fold reduction in the affinity of IGFBP 5 for IGF 1 . ^^^ Likewise , the ability of IGFBP 5 to inhibit IGF 1 stimulated receptor phosphorylation was attenuated . ^^^ In summary , residues 68 , 69 , 70 , 73 , and 74 in IGFBP 5 appear to be critical for high affinity binding to IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Following its secretion , IGFBP 5 was degraded , but the effect of IGF 1 on IGFBP 5 peptide abundance in conditioned media did not seem to be due to inhibition of proteolysis . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In this article we propose a role for IGFBP 5 in the mammary gland involving the initiation of apoptosis induced by sequestration of IGF 1 , an important survival factor for the mammary gland . ^^^ These observations make it likely that IGFBP 5 is capable of preventing interaction of IGF 1 with its receptor on the epithelial cells synthesizing milk . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
BACKGROUND : We have previously shown that insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 5 ( IGFBP 5 ) induce mesangial cell migration using separate stimulatory and effector pathways . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Human intestinal smooth muscle in culture produces insulin like growth factor ( IGF ) 1 and IGF binding protein ( IGFBP ) 3 , IGFBP 4 , and IGFBP 5 , which modulate the effects of IGF 1 . ^^^ R 3 IGF 1 , an agonist unaffected by IGFBPs , elicited concentration dependent increase in growth , measured by [ ( 3 ) H ] thymidine incorporation , and production of IGFBP 3 , IGFBP 4 , and IGFBP 5 , measured by Western blot . ^^^ Antagonists of the IGF 1 receptor , IGF 1 Analog or monoclonal antibody 1H7 , elicited concentration dependent inhibition of growth and decrease in IGFBP 3 , IGFBP 4 , and IGFBP 5 production , implying that endogenous IGF 1 stimulated growth and IGFBP production . ^^^ R 3 IGF 1 induced increase in IGFBP 3 , IGFBP 4 , and IGFBP 5 production was partially inhibited by a mitogen activated protein ( MAP ) kinase or a phosphatidylinositol 3 kinase ( PI 3 kinase ) inhibitor and abolished by the combination . ^^^ We conclude that endogenous IGF 1 stimulates growth and IGFBP 3 , IGFBP 4 , and IGFBP 5 production in human intestinal smooth muscle cells . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 5 ( IGFBP 5 ) stimulates osteoblast proliferation directly or indirectly through IGF 1 action , but its effects on osteoclast formation and osteoclastic activity are unknown . ^^^ IGFBP 5 significantly stimulated pit formation by pre existent osteoclasts in mouse bone cell cultures and its stimulatory effect was completely blocked by IGF 1 antibody ( Ab ) . ^^^ When IGFBP 5 was added to unfractionated bone cells after degeneration of pre existent osteoclasts , IGFBP 5 ( 77 pM 7 . 7 nM ) dose dependently stimulated osteoclast like cell formation , irrespective of the presence of IGF 1 Ab . ^^^ IGFBP 5 dose dependently stimulated osteoclast like cell formation from osteoclast precursors , irrespective of the presence of IGF 1 Ab . ^^^ The present data indicate that IGFBP 5 stimulates bone resorption both by stimulation of osteoclast formation in an IGF 1 independent fashion and by IGF 1 dependent activation of mature osteoclasts , possibly via osteoblasts , in vitro . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
An IGF 1 analog with reduced affinity for IGFBPs , Long R 3 IGF 1 , also induced IGFBP 5 , while insulin was less effective ( 2 . 2 fold stimulation at 10 nM ) . ^^^ IGF 1 protected IGFBP 5 against proteolytic degradation by conditioned medium . ^^^ IGF 1 also enhanced the level of IGFBP 5 mRNA . ^^^ LY 294002 , a specific inhibitor of the intracellular signaling molecule phosphatidylinositol 3 kinase , inhibited stimulation of IGFBP 5 by IGF 1 . ^^^ IGFBP 5 has been shown in several cell types to enhance the mitogenic activity of IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To analyze the functional significance of IGFBP 5 overexpression in IGF 1 mediated mitogenesis and progression to androgen independence , IGFBP 5 overexpressing human androgen dependent LNCaP prostate cancer cells were generated by stable transfection . ^^^ IGFBP 5 induced increases in LNCaP cell proliferation occurs through both IGF 1 dependent and independent pathways , with corresponding increases in the cyclin D 1 messenger RNA expression and the fraction of cells in S + G2 / M phases of the cell cycle . ^^^ Although treatment with a PI3K inhibitor induced apoptosis in both control and IGFBP 5 overexpressing LNCaP cells , this PI3K inhibitor induced apoptosis was prevented by exogenous IGF 1 treatment only in IGFBP 5 transfectants , suggesting that IGFBP 5 overexpression can potentiate the antiapoptotic effects of IGF 1 . ^^^ Collectively , these data suggest that IGFBP 5 overexpression in prostate cancer cells after castration is an adaptive cell survival mechanism that helps potentiate the antiapoptotic and mitogenic effects of IGF 1 , thereby accelerating progression to androgen independence through activation of the PI3K Akt / protein kinase B signaling pathway . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Growth of Shionogi tumor cells was inhibited by antisense IGFBP 5 ODN treatment in a time and dose dependent manner , which could be reversed by exogenous IGF 1 . ^^^ However , antisense IGFBP 5 ODN treatment had no additive inhibitory effect on Shionogi tumor cell growth when IGF 1 activity was neutralized by anti IGF 1 antibody . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Differential effects of IGF binding proteins , IGFBP 3 and IGFBP 5 , on IGF 1 action and binding to cell membranes of immortalized human chondrocytes . ^^^ The stimulatory effect of IGF 1 was inhibited significantly by addition of IGFBP 3 but enhanced slightly by IGFBP 5 . ^^^ Binding of ( 125 ) 1 labeled IGF 1 to the cells was displaced by both IGFBP 3 and IGFBP 5 , although higher concentrations of unlabeled IGFBP 5 were required to displace IGF 1 to the same extent as IGFBP 3 . ^^^ Treatment of the cells with IGF 1 increased the levels of IGFBP 5 protein measured by Western ligand blotting , and stimulated a corresponding increase in IGFBP 5 mRNA while increasing type 2 collagen mRNA . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Serum IGF 1 , and its binding proteins ( IGFBP 3 , IGFBP 4 , and IGFBP 5 ) , as well as bone mineral density ( BMD ) at the lumbar spine , femoral neck , and radius midshaft were measured before and 1 year after antithyroid ( methimazole ) treatment . ^^^ Serum free thyroxine showed a positive correlation with serum levels of IGF 1 ( r = 0 . 73 , p < 0 . 05 ) , IGFBP 3 ( r = 0 . 59 , p < 0 . 05 ) , and IGFBP 4 ( r = 0 . 67 , p < 0 . 05 ) but not IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Prolactin plays an essential role since it suppresses the secretion of IGFBP 5 which would otherwise inhibit IGF 1 action and lead to the induction of cell death . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We have used the mouse mutant Hypodactyly ( Hd ) as a tool to determine the role of IGF 1 and two key IGF binding proteins ( IGFBP 2 and IGFBP 5 ) during embryonic development . ^^^ We show that all three genes , IGF 1 , IGFBP 2 and IGFBP 5 , display restricted expression patterns during limb development . ^^^ IGF 1 expression is downregulated in Hd limb buds in regions undergoing high levels of cell death , consistent with its proposed role as an anti apoptotic factor , while IGFBP 5 is found at higher levels in regions of cell death , consistent with reports of its association with apoptosis in adult tissues . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
GH acts by increasing IGF 1 whilst prolactin acts by inhibiting the expression of IGFBP 5 from the mammary epithelium . ^^^ During mammary involution , when serum prolactin levels decline , IGFBP 5 expression is dramatically upregulated and it binds with high affinity to IGF 1 preventing IGF 1 interaction with the IGF receptor and thus leading to epithelial cell apoptosis . ^^^ We thus hypothesized that IGFBP 5 could activate cell death by sequestration of IGF 1 and activate plasminogen cleavage by sequestering PAI 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In addition , 12 pairs of significantly coexpressed IGF system components were detected ( IGF 1 < > IGF 1R , IGF 1 < > IGF 2R , IGF 1 < > IGFBP 3 , IGF 1 < > IGFBP 6 , IGFBP 3 < > IGF 1R , IGFBP 6 < > IGF 1R , IGFBP 1 < > IGF 2R , IGFBP 3 < > IGF 2R , IGFBP 5 < > IGF 2R , IGFBP 3 < > IGFBP 5 , IGFBP 3 < > IGFBP 6 , IGFBP 5 < > IGFBP 6 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 increases abundance of IGFBP 5 mRNA in rat intestinal smooth muscle cells ( RISM ) , and IGFBP 5 protein in RISM conditioned media . ^^^ We studied the mechanism of IGF 1 ' s effect on IGFBP 5 mRNA , and the role of cytoplasmic proteins in modulating IGFBP 5 mRNA abundance . ^^^ IGF 1 increased IGFBP 5 nuclear transcripts by reverse transcription polymerase chain reaction ( RT PCR ) , suggesting that IGF 1 acts at least partially by increasing IGFBP 5 mRNA transcription . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Because IGFBP 5 has been shown to regulate IGF 1 actions , understanding the chemical identity and regulation of this protease is important for understanding how IGF 1 stimulates anabolic functions . ^^^ The findings define a mechanism for cleaving IGFBP 5 in the culture medium , thus allowing release of IGF 1 to cell surface receptors . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Our aim was to characterize the basis for this disparity in enterotrophic action by assessing the relationships between stimulation of intestinal growth , nutritional adequacy , and localization of expression of IGF 1 , insulin like growth factor binding protein ( IGFBP ) 3 and IGFBP 5 mRNAs in jejunum . ^^^ Localization of expression of IGF 1 , IGFBP 3 , and IGFBP 5 mRNAs in jejunum was accomplished by in situ hybridization . ^^^ IGF 1 strongly stimulated IGFBP 5 expression in the lamina propria and the muscularis and induced a twofold increase in IGFBP 5 mRNA based on RNase protection assay of intact jejunum total RNA . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment of confluent C 6 cells with 200 microg / ml Poly ( IC ) for 24 h decreased levels of immunoreactive IGF 1 in conditioned medium ( CM ) to 55 % of the control value , decreased IGF 1 receptor beta subunit levels to 28 % of the control value , and decreased levels of IGFBP 3 , IGFBP 4 , and IGFBP 5 protein in CM to 45 % , 50 % , and 30 % of the control values , respectively . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Differential regulation by FSH and IGF 1 of extracellular matrix IGFBP 5 in bovine granulosa cells : effect of association with the oocyte . ^^^ Treatment of OCCs and not MGCs with IGF 1 and FSH together increased extracellular matrix IGFBP 5 by 2 . 5 fold . ^^^ The differential regulation of extracellular matrix IGFBP 5 in OCCs compared to MGCs suggest involvement of changes in the extracellular matrix brought about by IGF 1 and FSH in overall regulation of IGF 1 in the ovarian follicle . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Previous studies have established the ability of IGF 1 to promote the elaboration of granulosa cell derived IGFBP 5 and to suppress the activity of granulosa cell derived IGFBP 5 directed endopeptidase . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Human intestinal smooth muscle cells in culture produce insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 4 , and IGFBP 5 , which can modulate the effects of IGF 1 on growth . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
GH increases insulin like growth factor 1 ( IGF 1 ) synthesis whereas PRL suppresses the production of insulin like growth factor binding protein 5 ( IGFBP 5 ) in the epithelial cells , which would otherwise inhibit IGF mediated cell survival . ^^^ IGFBP 5 was present in milk from involuting glands at high concentrations ( approximately 60 microg / ml ) and had a high affinity ( 8 . 03 10 10 ( 10 ) M ) for IGF 1 , suggesting an inhibitory effect of IGFBP 5 in the mammary gland . ^^^ We propose that GH and PRL inhibit apoptosis and ECM remodelling by a process that involves the control of IGF 1 and PAI 1 availability by IGFBP 5 , thus allowing these processes to be tightly coordinated . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 and / or IGFBP 5 seemed to be involved in the estrogen induced modulation of PTH action on osteoblast proliferation and function . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To evaluate this hypothesis further , we evaluated in vitro and in vivo effects of IGFBP 5 on bone formation parameters using the IGF 1 knockout ( KO ) mouse . ^^^ Treatment of serum free cultures of osteoblast clones derived from IGF 1 KO mice with recombinant human IGFBP 5 increased both proliferation and alkaline phosphatase ( ALP ) activity in a dose dependent manner , an effect comparable to that seen with IGF 1 . ^^^ To eliminate possible actions of IGF 2 , if any was produced by osteoblasts derived from IGF 1 knockout mice , the IGFBP 5 effect was studied in the presence of exogenously added IGFBP 4 , a potent inhibitor of IGF 2 actions in bone cells . ^^^ Addition of IGFBP 4 blocked IGF 1 but not IGFBP 5 induced cell proliferation in osteoblasts derived from IGF 1 knockout mice . ^^^ Consistent with in vitro results , a single local injection of IGFBP 5 to the outer periosteum of the parietal bone of IGF 1 KO mice increased ALP activity and osteocalcin levels of calvarial bone extracts . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We also show that IGF 1 increases IGFBP 5 expression in CGCs and that the downregulation of IGFBP 5 mRNA can be suppressed by inhibiting mRNA synthesis with actinomycin D . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In rat colonic smooth muscle cells , IGF 1 increased collagen 1 and IGFBP 5 expression . ^^^ In colonic smooth muscle cells , we found an upregulation of collagen 1 and IGFBP 5 following IGF 1 incubation . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
A similar result was obtained with long [ R 3 ] IGF 1 which has a low affinity for all IGFBPs , suggesting that the inhibitory effect of IGFBP 5 is only partially IGF dependent . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We measured concentrations of immunoreactive IGF 1 in plasma , jejunum , and colon , IGF 1 receptor binding , and levels of IGF receptor , IGF 1 , IGF binding protein ( IGFBP ) 3 and IGFBP 5 mRNA in residual jejunum and colon 7 days after resection and / or IGF 1 treatment . ^^^ Resection induced colonic growth in association with significantly greater colonic IGFBP 5 mRNA and significantly lower colonic immunoreactive IGF 1 . ^^^ IGF 1 treatment significantly increased IGFBP 5 mRNA in the jejunum , which also correlated with jejunal growth . ^^^ Thus resection upregulated IGF 1 receptor number and IGF 1 mRNA in residual jejunum and colon , but differential adaptation of these segments correlated with differential regulation of IGFBP 5 mRNA . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In contrast , intact IGFBP 5 was stimulatory in the absence or presence of exogenous IGF 1 , whereas the amino terminal fragment IGFBP 5 ( 1 169 ) was inhibitory . ^^^ Studies on the mechanism by which IGFBP 4 and IGFBP 5 exert opposite effects on chondrocyte proliferation demonstrated that intact IGFBP 4 prevented the binding of ( 125 ) 1 IGF 1 to chondrocytes , whereas intact IGFBP 5 enhanced ligand binding and was able to bind specifically to the cell membrane . ^^^ IGFBP 5 , however , can either stimulate ( if it remains intact ) or inhibit ( if amino terminal forms predominate ) IGF 1 stimulated chondrocyte proliferation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 stimulated thymidine incorporation and modified the pattern of IGFBPs , decreasing the inhibitory IGFBP 4 through down regulation of its mRNA , and increasing IGFBP 5 which , per se , was able to modulate HREC growth , exerting post transcriptional control . ^^^ Studies using an antibody ( alpha IR 3 ) against the IGF 1 receptor , and compounds with low affinity for IGFBPs , such as insulin and des ( 1 3 ) IGF 1 , showed that an interaction between IGF 1 and IGFBP 5 was necessary to detach this IGFBP from its binding sites . ^^^ The dose of IGF 1 that significantly decreased the IGFBP 4 / IGFBP 5 ratio was the same that stimulated HREC growth . ^^^ These results extend our previous observations in endothelial cells and suggest that the IGFBP 4 / IGFBP 5 ratio regulates IGF 1 induced growth of HRECs , whereas a general decrease in IGFBPs ( except for IGFBP 4 ) was the anti proliferative effect of chronic exposure to high glucose concentrations . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We conclude that IGF 1R and IGFBP 5 are thyroid hormone target genes in human osteoblasts , whereas IGF 1 mRNA expression itself appears not to be regulated by T ( 3 ) in hOB . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In parallel , clenbuterol administration induced in soleus muscle a fivefold increase in IGF 1 mRNA levels associated with an eightfold increase in IGF binding protein ( IGFBP ) 4 and a fivefold increase of IGFBP 5 mRNA levels on day 3 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These results suggest that the loss of IGFBP 5 and possibly IGF R 2 , both of which can sequester IGF 1 from IGF R 1 , permits unhindered proliferation of the premalignant EVT in an IGF 1 rich environment of the fetal maternal interface . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Vitronectin binding to IGF binding protein 5 ( IGFBP 5 ) alters IGFBP 5 modulation of IGF 1 actions . ^^^ IGF binding protein 5 ( IGFBP 5 ) and vitronectin are matricellular proteins that are produced by smooth muscle cells and modulate their responsiveness to IGF 1 . ^^^ IGF binding protein 5 ( IGFBP 5 ) and vitronectin are matricellular proteins that are produced by smooth muscle cells and modulate their responsiveness to IGF 1 . ^^^ These studies were conducted to determine if vitronectin bound IGFBP 5 with high affinity and if this altered the ability of either protein to modify cellular responsiveness to IGF 1 . ^^^ When the combination of IGFBP 5 and IGF 1 was added to smooth muscle cells that had been plated on a vitronectin enriched matrix , the smooth muscle cell DNA synthesis and migration responses to IGF 1 plus vitronectin were enhanced . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
When 20 ng / mL of IGF 1 was added to the media , IGFBP 3 was increased approximately 65 % ( P < 0 . 05 ) and IGFBP 5 was increased approximately twofold ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Conversely , the gene expression of IGFBP 5 was upregulated in stretched cells , and neutralizing antibodies to IGF 1 blocked this activation . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
RESULTS : The expression of IGF 1 , IGF 2 , IGF binding protein 5 ( IGFBP 5 ) , and CTGF ( IGFBP rP 2 ) was seen in a higher proportion of mononuclear cells of patients with ALL than in controls . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Roles of insulin like growth factor 1 ( IGF 1 ) and IGF 1 binding protein 2 ( IGFBP 2 ) and 5 ( IGFBP 5 ) in developing chick limbs . ^^^ We have examined the distribution of IGF 1 , IGFBP 2 and IGFBP 5 in developing chick limb buds and have investigated their functional roles and relationships during chick limb development . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Since C1s degrades insulin like growth factor binding protein 5 ( IGFBP 5 ) , we undertook to determine whether inhibiting C1s in joint fluid would result in an increase in the amount of intact IGFBP 5 and IGF 1 in cartilage and joint fluid , and whether C1s inhibition would be associated with a reduction in cartilage destruction during the development of osteoarthritis ( OA ) . ^^^ This increase in concentrations of intact IGFBP 3 and IGFBP 5 leads to an increase in IGF 1 which is associated with an improvement in joint architecture during the development of OA . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 5 ( IGFBP 5 ) stimulates growth and IGF 1 secretion in human intestinal smooth muscle by Ras dependent activation of p 38 MAP kinase and Erk1 / 2 pathways . ^^^ Insulin like growth factor binding protein 5 ( IGFBP 5 ) and insulin like growth factor 1 ( IGF 1 ) are produced by human intestinal smooth muscle cells . ^^^ Endogenous IGF 1 stimulates growth and increases IGFBP 5 secretion . ^^^ IGFBP 5 augments the effects of IGF 1 by facilitating interaction of IGF 1 with the IGF 1 receptor tyrosine kinase . ^^^ K . ( 2000 ) Kidney Int . 57 , 1991 2003 ) have shown that in osteoblasts and kidney mesangial cells , IGFBP 5 stimulates proliferation and filopodia formation independently of IGF 1 , presumably by activating a distinct IGFBP 5 receptor serine kinase . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The purpose of our investigation was to determine the possible role of PRL , GH , and IGF 1 on cell survival and on IGFBP 5 expression in the bovine mammary gland . ^^^ The results show a high level of DNA laddering and an increase in IGFBP 5 mRNA content in mammary explants cultured in the absence of PRL , GH , or IGF 1 with respect to explants treated with all hormones . ^^^ Moreover , explants cultured in presence of PRL , GH , or IGF 1 show a low level of DNA laddering and IGFBP 5 expression with respect to explants cultured without any hormones . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Upon conditional expression of c Myb in a NB cell line , we detected up regulation of IGF 1 , IGF 1 receptor , and insulin like growth factor binding protein 5 ( IGFBP 5 ) expression . ^^^ Together , these data suggest that the Myb binding independent transactivation of the IGFBP 5 promoter was due to the activation of the phosphatidylinositol 3 kinase / AKT pathway likely mediated by IGF 1 receptor dependent signals . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These results indicate that IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 5 are produced by equine granulosa cells and that insulin , FSH , and estradiol play a role in the regulation of steroidogenesis and the IGF system of equine granulosa cells . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 5 , osteocalcin , OPG , RANKL , and total protein were determined in extracts obtained after EDTA and guanidine hydrochloride extraction . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In the presence of mutant IGFBP 5 , however , IGF 1 no longer conferred survival and in the presence of wild type IGFBP 5 , long R 3 IGF 1 was also unable to confer survival . ^^^ In MCF 7 cells , IGF 1 induced survival could be facilitated by IGFBP 5 , but also blocked by IGFBP 5 if association with IGFBP 5 was prevented . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
A period of 20 min of H / I that resulted in histopathology in cortex , striatum , and thalamus was correlated with induction of mRNA for IGF 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and glial fibrillary acidic protein ( GFAP ) by 4 days of recovery . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Treatment with DXM ( 100 nM ) resulted in a 2 fold increase in mRNA levels of both IGFBP 5 and the type 1 IGF receptor , whereas IGFBP 2 mRNA levels decreased by 55 % , in concert with the decrease in protein level observed under IGF 1 supplemented culture conditions . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To test whether two major IGF 1 binding proteins , IGFBP 4 and IGFBP 5 , are related to bone mineral density ( BMD ) , we studied a sample of the Framingham Offspring Cohort participants ( 99 men and 101 women , ages 60 87 ) . ^^^ Serum levels of IGF 1 , IGFBP 4 , and IGFBP 5 were measured by previously validated radioimmunoassays ( CVs approximately 10 % ) . ^^^ In males , but not females , IGF 1 and IGFBP 5 were inversely associated with age ( r = 0 . 34 and r = 0 . 28 , respectively ; P < 0 . 01 ) , while IGFBP 4 levels were positively associated with age ( P < 0 . 01 ) . ^^^ In women , but not men , IGFBP 5 was positively associated with femoral neck BMD ( P = 0 . 03 ) , however , after statistical adjustment for IGF 1 , this association was no longer significant . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We found transcriptional upregulation of both IGF 1 and IGF 2 in dystrophic muscle , however the possible beneficial effects of the growth factors appear offset by transcriptional upregulation of inhibitory IGF binding proteins and regulators ( IGFBP 2 , 4 , 6 and 7 ; and PRSS 11 [ IGFBP 5 protease ] ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In order to examine whether IGFBP 5 acts by inhibiting the survival effect of IGF 1 we examined IGF receptor phosphorylation and Akt phosphorylation and showed that both were inhibited . ^^^ We attempted to `` rescue ' ' the transgenic phenotype by using growth hormone to increase endogenous IGF 1 concentrations or by implanting minipumps delivering an IGF 1 analogue , R ( 3 ) IGF 1 , which binds weakly to IGFBP 5 . ^^^ Growth hormone treatment failed to affect mammary development suggesting that increased concentrations of endogenous IGF 1 are insufficient to overcome the high concentrations of IGFBP 5 produced by these transgenic animals . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Thus , we propose that a reduction in the expression of the PRLR may lead to increased IGFBP 5 expression ( proapoptotic ) and that GH may rescue mammary development by increasing IGF 1 , an important mitogen and survival factor for the mammary epithelium . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Circulating IGF 1 and IGFBP 5 showed similar age dependent responses to refeeding , rising significantly faster in juveniles . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In silico and NMR identification of inhibitors of the IGF 1 and IGF binding protein 5 interaction . ^^^ Recently we have determined the crystal structure of the insulin like growth factor 1 ( IGF 1 ) in complex with the N terminal domain of the IGF binding protein 5 ( IGFBP 5 ) . ^^^ Small molecular weight compounds ( FMOC derivatives ) bind to the IGF 1 binding site on the IGFBP 5 with micromolar affinities and thus serve as potential starting compounds for the design of more potent inhibitors and therapeutic agents for diseases that are associated with abnormal IGF 1 regulation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Addition of exogenous IGFBP 5 and IGFBP 3 to MECs suppressed IGF 1 mediated survival , resulting in threefold greater apoptosis in cells incubated with IGF 1 and IGFBP 5 compared with IGF 1 alone . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
To evaluate whether changes in these proteins may to some extent explain the decrease in bone mass in postmenopausal or senile osteoporosis , we measured bone contents of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 5 , and OPG in combined extracts obtained after EDTA and guanidine hydrochloride extraction in 60 postmenopausal women aged 47 74 ( mean , 63 ) yr with a previous distal forearm fracture and a hip or spine Z score less than 0 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Compared with controls , immunoreactivity in the spinal motor neurons of patients with amyotrophic lateral sclerosis was 64 % higher for IGFBP 2 , 46 % higher for IGFBP 5 , and 33 % higher for IGFBP 6 , with upregulation of IGF 1 receptors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Ribonuclease protection assays were used to measure steady state semimembranosus muscle and / or hepatic levels of IGF 1 , IGFBP 3 , IGFBP 5 , hepatocyte growth factor ( HGF ) , and myostatin messenger RNA ( mRNA ) in steers implanted from 32 to 38 d with Revalor S , a combined trenbolone acetate and estradiol implant . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Interestingly , levels of IGFBP 5 were decreased in the OC BP 4 mice , possibly because of a compensatory alteration in IGF 1 action . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Expression of IGF 1 , IGFBP 3 and IGFBP 5 mRNA did not differ between CFP and MFP , suggesting that expression of these factors was not influenced by interactions between stroma and developing epithelium . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We report that these genes fall into two distinct groups with respect to expression pattern , with IGFBP 2 displaying broad overlap of mRNA expression with IGFR and IGF 1 during early development , whereas the expression profile of IGFBP 5 most closely resembled that of IGF 2 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF binding protein 5 ( IGFBP 5 ) is an important trophic factor for controlling the actions of IGF 1 in human dermal fibroblasts and porcine aortic smooth muscle cells . ^^^ IGF binding protein 5 ( IGFBP 5 ) is an important trophic factor for controlling the actions of IGF 1 in human dermal fibroblasts and porcine aortic smooth muscle cells . ^^^ When IGFBP 5 is associated with extracellular matrix , it acts to enhance the cell growth response to IGF 1 . ^^^ This increase results in a net increase in IGFBP 5 proteolysis , which has the potential to modify IGF 1 and IGFBP 5 actions . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 4 , IGFBP 5 , and nonglycosylated IGFBP 3 were shown to significantly enhance binding of IGF 1 to VN , whereas IGFBP 2 and glycosylated IGFBP 3 had a smaller effect . ^^^ To examine the functional significance of IGFs binding to VN , cell migration in MCF 7 cells was measured and found to be enhanced when VN was prebound to IGF 1 in the presence of IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In contrast , levels and / or sites of expression of IGF 1 , IGF 2 , IGFBP 4 , IGFBP 5 and type 2 receptor in follicular cells strongly differ between mammalian species , suggesting that these phenomena might play species specific or secondary roles in ovarian folliculogenesis . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Interestingly , the peptide uses the same binding site as detergent and a fragment of IGFBP 5 identified in other IGF 1 complexes . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 had an inhibitory effect on IGF 1 stimulated DNA synthesis , but it strongly potentiated IGF 1 induced VSMC migration . ^^^ By using a non IGF binding IGFBP 5 mutant and an IGF 1 neutralizing antibody , it was demonstrated that IGFBP 5 also stimulates VSMC migration in an IGF independent manner . ^^^ These findings suggest that local IGFBPs are important determinants of cellular responses to IGF 1 stimulation , and a key player in this paradigm is IGFBP 5 . ^^^ IGFBP 5 not only modulates IGF 1 actions , but it also stimulates cell migration by interacting with cell surface heparan sulfate proteoglycans . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These results suggest that the insulin deficiency and chronic hyperinsulinemia in diabetes upregulate the IGF 1 receptor and downregulate IGFBP 4 and IGFBP 5 in the aorta . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The transcript level of upstream signaling molecules , such as IGF binding protein 5 ( IGFBP 5 ) , IGF 1 receptor ( IGF 1R ) , and phosphoinositide 3 kinase ( PI3K ) , was studied using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Furthermore , the role of IGFBP 5 in IGF 1 signaling was verified by annexin 5 staining using flow cytometric analysis . ^^^ In the presence of exogenous IGFBP 5 , the annexin 5 positive cells were significantly decreased in GF after IGF 1 stimulation . ^^^ In addition , the anti apoptotic effect of IGF 1 may be facilitated by the upregulation of IGFBP 5 in PDLF . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The transcripts of IGF 1 , IGF 2 , IGF receptor ( IGF R ) , two IGF binding proteins ( IGFBP 2 and IGFBP 5 ) , GH receptor ( GH R ) and insulin receptor ( 1 R ) were measured by RT PCR at 4 , 8 , 12 and 16 weeks of age in the ovaries of ad libitum fed and feed restricted hens . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In the current study , the soluble IGFBP 5 secreted by BC ( 3 ) H 1 cells is shown to bind approximately 50 % more [ ( 125 ) 1 ] IGF 2 than [ ( 125 ) 1 ] IGF 1 at pH 7 . 4 . ^^^ Zn ( 2+ ) is shown to depress the binding of both IGF 1 and IGF 2 to soluble secreted IGFBP 5 ; [ ( 125 ) 1 ] IGF 1 binding is affected more so than [ ( 125 ) 1 ] IGF 2 binding . ^^^ By depressing the association of the IGFs with soluble IGFBPs , Zn ( 2+ ) is shown to repartition either [ ( 125 ) 1 ] IGF 1 or [ ( 125 ) 1 ] IGF 2 from soluble IGFBP 5 onto cell surface IGF receptors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Thus , IGF 1 , IGF 2 , IGFR 2 , and IGFBP 5 seem to play a role in the atrophy of mouse masseter muscle in response to the change from a hard to a soft diet in an autocrine and / or paracrine manner . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 potentiated IGF 1 induced cell migration in FN null , but not in wild type , mouse embryonic cells . ^^^ When FN was reintroduced either as an adhesive substrate or in solution to the FN null cells , the potentiating effect of IGFBP 5 on IGF 1 induced cell migration was abolished . ^^^ Binding of IGFBP 5 to FN had no effect on the ability of IGFBP 5 to bind IGF 1 , but it increased the proteolytic degradation of IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Zinc ( Zn ( 2+ ) ) , a multifunctional micronutrient , was recently shown to lower the affinity of cell associated insulin like growth factor ( IGF ) binding protein ( IGFBP ) 3 and IGFBP 5 for both IGF 1 and IGF 2 , but to increase the affinity of the cell surface type 1 IGF receptor ( IGF 1R ) for the same two ligands . ^^^ Zn ( 2+ ) , at physiological levels , depressed binding of both IGF 1 and IGF 2 to IGFBP 5 , affecting ( 125 ) 1 IGF 1 more than ( 125 ) 1 IGF 2 . ^^^ Both ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 bound to high and low affinity sites on IGFBP 5 . ^^^ An IGF 1 analog , ( 125 ) 1 R ( 3 ) IGF 1 , did not bind to the soluble murine IGFBP 5 . ^^^ Soluble IGFBP 5 and IGFBP 4 depressed the binding of ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 to the IGF 1R , but did not affect binding of ( 125 ) 1 R ( 3 ) IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
This pro apoptotic action of exogenous IGFBP 5 was abolished when IGF 1 was added in excess , suggesting that exogenous IGFBP 5 increases apoptosis by binding to and inhibiting the activities of insulin like growth factors . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These changes may be mediated by insulin like growth factors ( IGF 1 and IGF 2 ) , but the matrix bound binding protein , IGFBP 5 has not been investigated . ^^^ OBJECTIVES : To measure IGF 1 , IGF 2 , and IGFBP 5 in femoral head bone from non OA controls and patients with OA , and to relate these to apparent density ( rhoA ) and elastic modulus ( Ec ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Although the hIGF 2 transgene is expressed in neuronal structures similar to IGF 1 and IGFBP 5 , it is not able to regulate IGFBP 5 expression , as has previously been reported for IGF 1 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The actions of IGF 1 are modulated by a family of binding proteins ( IGFBPs ) and we have shown that IGFBP 5 is associated with cell death in the mammary gland and more recently provided the first evidence that it is causally related to apoptosis of the mammary gland . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We analyzed the histology by optic light microscopy , activities of antioxidant enzymes ( CAT , SOD , GPx and G 6 PDH ) , expression of iNOS and COX 2 by Western blot , expression of MT 1 , MT 2 , IGF 1 , IGF BP 5 and rtert by RT PCR . ^^^ MT 1 and IGF BP 5 mRNA increased while MT 2 , IGF 1 and rtert did not show variations . ^^^ The histology suggests an involution state of the gland , confirmed by the expression of IGF 1 , IGF BP 5 and rtert . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
MEASUREMENTS : We analysed the strength and independence of associations of angiographically assessed presence of CHD with BMI , systolic and diastolic blood pressure , total , high density lipoprotein ( HDL ) and LDL cholesterol , triglycerides , lipoprotein ( a ) , apolipoproteins A 1 and B , total and free IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 5 , acid labile subunit ( ALS ) , insulin , C peptide , testosterone , DHEAS and sex hormone binding globulin . ^^^ RESULTS : Using multivariate statistical analysis , the presence of CHD had significant positive associations with total IGF 1 , IGFBP 5 , ALS and IGFBP 3 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Inhibition of the activity of the protease following its secretion by B 104 cells resulted in inhibition of IGFBP 5 proteolysis and IGF 1 stimulation of protein synthesis . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In large follicle ( > 7 . 9 mm ; Experiment 2 ) granulosa cells , 100 ng / ml of IGF 1 increased ( P < 0 . 05 ) IGFBP 2 mRNA levels above controls and 3 ng / ml of IGF 1 ; 100 ng / ml of IGF 1 also decreased ( P < 0 . 10 ) IGFBP 5 mRNA levels compared to 3 ng / ml of IGF 1 or FSH or 100 ng / ml leptin , while 100 ng / ml of IGF 2 had no effect ( P > 0 . 10 ) on IGFBP 2 , 3 , 4 , and 5 mRNA levels ( Experiment 2 ) . ^^^ In contrast , IGF 1 but not IGF 2 increased ( P < 0 . 01 ) thecal IGFBP 5 mRNA levels . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
On the other hand , exogenous IGF 1 induced 8 11 % ( p < 0 . 05 ) increases in the proliferation , but cyclosporin A induced 30 80 % ( p < 0 . 05 0 . 01 ) reductions in the mRNA expression levels for endogenous IGF 1 , IGFR 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
RESULTS : Clear cell RCC were characterized by significant increases in the mRNA expression of IGF 1 , IGFBP 3 and IGFBP 6 while papillary RCC exhibited down regulated expression of IGF 1 , IGFBP 4 and IGFBP 5 . ^^^ Semiquantitative assessment of immunohistochemical staining demonstrated significant increases in epithelial associated IGF 1 and IGFBP 3 in clear cell RCC , increased IGFBP 5 protein in papillary RCC and no significant changes in IGF / IGFBP protein expression in oncocytoma . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
There is little detail on the nature of the molecular complex formed between ECM components , IGFBPs and IGFs although the glycosaminoglycan ( GAG ) heparin has been reported to reduce the affinity of IGFBP 5 for IGF 1 . ^^^ The potential biological significance of our data is highlighted by the demonstration that IGF 1 and IGF 2 can displace endogenous IGFBP 5 from monolayer cultures of the mouse mammary epithelial cell line HC11 . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
While serum IGF 1 levels were not changed by DHT treatment , IGF 1 gene expression levels increased and the mRNA levels of IGFBP 3 and IGFBP 5 were suppressed in the LA muscle after DHT replacement in castrated rats . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 5 ( IGFBP 5 ) interacts with thrombospondin 1 to induce negative regulatory effects on IGF 1 actions . ^^^ The purpose of these studies was to determine how the interaction between IGFBP 5 and TS 1 modulates IGF 1 actions in porcine aortic smooth muscle cells ( pSMC ) in culture . ^^^ The addition of increasing concentrations of TS 1 to pSMC cultures enhanced the protein synthesis and cell migration responses to IGF 1 ; whereas the addition of IGFBP 5 alone resulted in minimal changes . ^^^ In contrast , the addition of IGFBP 5 to cultures that were also exposed to IGF 1 and TS 1 resulted in inhibition of protein synthesis . ^^^ When the cell migration response was assessed , the response to IGF 1 plus TS 1 was also significantly inhibited by the addition of IGFBP 5 , whereas 1 . 0 microg / ml of IGFBP 5 alone had no effect on the response to IGF I . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Furthermore , the addition of EGF and / or bFGF to IGF 1 : IGFBP 5 : VN complexes significantly enhances both [ ( 3 ) H ] leucine incorporation and migration of HaCAT cells above that induced by IGF : IGFBP 5 : VN complexes alone . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Bone IGFBP 5 and IGF 1 mRNA abundance was determined using real time reverse transcription ( RT ) PCR . ^^^ The elevated IGFBP 5 protein levels were associated with a similar increase in IGF 1 mRNA abundance ( 4 fold , P < 0 . 01 ) and a significant increase in IGFBP 5 mRNA abundance ( 1 . 5 fold ) . ^^^ These studies demonstrate a distinctive developmental pattern of IGFBP 5 content in mouse bone and show that osteoblast derived IGF 1 determines skeletal IGFBP 5 abundance , at least in part by inducing its synthesis . ^^^ In that IGFBP 5 is thought to stimulate bone formation , directly or via IGF 1 action , such changes in bone IGFBP 5 may be important to ensure robust bone acquisition in the early postnatal period . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Production of recombinant porcine IGF binding protein 5 and its effect on proliferation of porcine embryonic myoblast cultures in the presence and absence of IGF 1 and Long R 3 IGF 1 . ^^^ The goal of this study was to produce recombinant porcine IGFBP 5 ( rpIGFBP 5 ) and assess its IGF 1 dependent and IGF 1 independent actions on the proliferation of PEMCs . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In growth plate chondrocytes in primary culture , incubation with IGF 1 increased the concentrations of IGFBP 3 and IGFBP 5 in conditioned cell culture medium in a dose and time dependent manner . ^^^ Coincubation of IGF 1 with specific inhibitors of the p42 / 44 MAPK pathway ( PD 098059 or U 0126 ) completely abolished the stimulatory effect of IGF 1 on IGFBP 3 mRNA expression but did not affect increased IGFBP 5 mRNA levels . ^^^ The IGF 1 induced IGFBP 5 gene expression required de novo mRNA transcription and de novo protein synthesis . ^^^ These data suggest that IGF 1 modulates its activity in cultured rat growth plate chondrocytes by the synthesis of both inhibitory ( IGFBP 3 ) and stimulatory ( IGFBP 5 ) binding proteins . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Because IGFBP 5 has the capacity to block IGF 1 activity , we hypothesized that reduced IGF 1 availability resulting from excess IGFBP 5 accelerates the apoptosis of weaver granule neurons . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Expression of IGF 1 , IGF 2 , and IGF binding protein 5 mRNA was assessed by real time reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Comparison of our results with the crystal structure of the complex between IGF 1 and an N terminal fragment of IGFBP 5 allowed identification of those residues perturbed by the C domain of IGFBP 2 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 protein expression was evaluated by Western blot analysis . mRNA expression in microarray and real time RT PCR showed similar tendencies : IGF 1 , IGF R 1 , IGF R 2 , IR A , and IGFBP 2 were not different in both groups . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We proposed that growth hormone ( GH ) increases the survival factor IGF 1 , whilst prolactin ( PRL ) enhances the effects of GH by decreasing the concentration of IGFBP 5 , which would otherwise inhibit the actions of IGFs . ^^^ In order to examine whether IGFBP 5 acts by inhibiting the survival effect of IGF 1 , we examined IGF receptor and Akt phoshorylation and showed that both were inhibited . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGF 1 induced large dose dependent increases in IGFBP 3 mRNA and protein levels in BME cells , while IGFBP 5 protein was barely detectable and mRNA levels were detectable only by qRT PCR . ^^^ In BMFs , IGF 1 induced large increases in IGFBP 5 mRNA and protein while IGFBP 3 mRNA was only slightly increased by IGF 1 treatment and the protein was difficult to detect . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
No treatment effect was found for muscle IGF 1 ( P = 0 . 36 ) , IGF 2 ( P = 0 . 51 ) , IGFBP 3 ( P = 0 . 70 ) , or IGFBP 5 ( P = 0 . 51 ) mRNA abundance . ^^^ Uterine IGF 1 ( P = 0 . 46 ) , IGF 2 ( P = 0 . 40 ) , IGFBP 3 ( P = 0 . 29 ) , and IGFBP 5 ( P = 0 . 35 ) mRNA abundance did not differ between treatments . ^^^ Placental IGF 1 ( P = 0 . 30 ) , IGF 2 ( P = 0 . 18 ) , IGFBP 3 ( P = 0 . 94 ) , and IGFBP 5 ( P = 0 . 42 ) mRNA abundance did not differ between treatments . ^^^ There was no treatment difference for IGF 1 ( P = 0 . 31 ) or IGFBP 5 ( P = 0 . 13 ) in PEM . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The aim of this work was to analyze the effect of adjuvant induced arthritis on the muscle specific ubiquitin ligases muscle ring finger 1 ( MuRF 1 ) and muscle atrophy F box ( MAFbx ) as well as on IGF 1 and IGF binding protein 5 ( IGFBP 5 ) gene expression in the skeletal muscle . ^^^ Arthritis decreased the serum IGF 1 and its gene expression in the liver ( P < 0 . 01 ) , whereas it increased IGF 1 and IGFBP 5 gene expression in the skeletal muscle ( P < 0 . 01 ) . ^^^ GHRP 2 treatment increased the serum concentrations of IGF 1 and the IGF 1 mRNA in the liver and in the cardiac muscle and decreased muscular IGFBP 5 mRNA both in control and in arthritic rats ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In addition , IGF 1 mRNA was present in the cornea , IGFBP 1 mRNA was observed in the cornea and iris , and IGFBP 5 and 6 mRNAs were identified in the ciliary body , iris , and cornea . mRNAs for IGFBP 2 , 3 , and 4 were not found in the eyes . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Immunoreactivity of presumably endogenous IGFBP 5 was identified within proliferating rat L 6 myoblast nuclei using fluorescent and confocal microscopy in separate experiments and was reduced by 100 ng / ml IGF 1 in a time dependent manner . ^^^ Western blotting of nuclear and cytosolic protein identified a single anti IGFBP 5 immunoreactive protein of approximately 200 kDa , primarily in nuclear fractions , that was downregulated in cells treated with IGF 1 for 12 h . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We examined the ability of mutants to inhibit IGF mediated survival of MCF 7 cells and were able to demonstrate that this depended not only upon the affinity for IGF 1 , but also the kinetics of this interaction , because IGFBP 5 mutants with similar affinity constants ( K ( D ) ) values , but with different association ( Ka ) and dissociation ( Kd ) rate values , had markedly different inhibitory properties . ^^^ Instead , these C Term mutants appeared to enhance the actions of IGF 1 by a combination of increased dissociation of IGF IGFBP complexes from the substratum , together with dissociation of IGF 1 from IGFBP 5 bound to the substratum . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Although IGFBP 5 overexpression did not have an IGF independent effect on RCJ cell differentiation , it promoted IGF 1 enhanced differentiation of these cells . ^^^ A potential mechanism for this effect is the specific increase of Akt phosphorylation in IGFBP 5 overexpressing cells in the presence of IGF 1 , indicating an increased activity of the phosphatidylinositol ( PI ) 3 kinase pathway . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
SOM 230 did not reduce IGF 1 mRNA abundance in mammary gland but did stimulate IGF binding protein 5 ( IGFBP 5 ) . ^^^ We conclude that 1 ) SAs inhibit IGF 1 action in the mammary gland through a novel nonpituitary mechanism ; 2 ) IGFBP 5 , here shown to inhibit pubertal mammary development , might mediate the effect ; and 3 ) Measurement of available sstr receptors in the mammary gland suggests that sstr ( 3 ) mediates the SA activity , but sstr ( 5 ) is also a possible mediator . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Jejunal atrophy due to TPN and growth due to IGF 1 were directly associated with expression of IGFBP 5 mRNA . ^^^ TPN decreased IGFBP 5 mRNA by 60 % and IGF 1 increased IGFBP 5 mRNA by 200 % with no change in IGF 1 mRNA compared with oral feeding . ^^^ In summary , TPN induces significant 25 % atrophy of the mouse small intestine that is attenuated by IGF 1 in association with increased expression of IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The aim of the present paper was to evaluate the expression of growth factor IGF 1 , and growth factor binding proteins IGFBP 3 and IGFBP 5 genes and induction of NOS family members ( nNOS , eNOS and iNOS ) and TNF alpha genes together with glia activation , in the SN at 5 and 48 h after severe hypoxia in the 7 day old rat , a model for the term human fetus . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In a rat osteoblast cell culture that we studied , IGF 2 and IGFBP 2 were the most abundant peptides of the IGF system released in cell medium , IGFBP 5 was abundantly expressed but bound to cell surface and cell matrix and IGF 1 and IGFBP 3 were found at very low concentrations . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
A 24 h treatment with a single dosage of ionic products of sol gel 58S dissolution induced the differential expression of a number of genes , including IL 6 signal transducer / gp130 , ISGF 3 / STAT1 , HIF 1 responsive RTP 801 , ERK 1 p44 MAPK ( MAPK 3 ) , MAPKAPK 2 , IGF 1 and IGFBP 5 . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
In contrast , exogenous administration of IGF 1 as part of a binary complex composed of IGF I / IGFBP 3 produced comparable increases in IGFBP 5 mRNA in both control and septic muscle . ^^^ Concomitant determinations of IGF 1 mRNA content revealed a positive linear relationship between IGF 1 and IGFBP 5 mRNA in the same muscle in response to LPS , sepsis , TNF alpha , and GH treatment . ^^^ Finally , IGF 1 appears to be a dominant positive regulator of IGFBP 5 gene expression in muscle under both normal and catabolic conditions . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
We investigated the actions of DHT , T , DHEA , and estradiol ( E 2 ) on insulin like growth factor ( IGF ) 1 , IGF 2 , IGF 1 receptor ( R ) , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 5 in primary cultures of human prostatic stromal cells by assessing cell proliferation , mRNA expression , and protein secretion by MTT growth assay , quantitative real time PCR , and ELISA , respectively . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
These cells were seeded on tissue culture plates and porous scaffolds of type 1 collagen sponges and polyglycolic acid ( PGA ) , which had been coated with VN + / IGFBP 5 + / IGF 1 . ^^^ However , coating the scaffolds with complexes composed of VN + IGF 1 or VN + IGFBP 5 + IGF 1 enhanced cell attachment on PGA . ^^^ Moreover , the presence of VN + IGFBP 5 + IGF 1 resulted in significantly greater osteoblast migration into deep pore areas as compared with untreated scaffolds or scaffolds treated with fetal calf serum . ^^^ These results demonstrated that complexes of VN + IGFBP 5 + IGF 1 can be used to expand osteoblasts in vitro under serum free conditions and enhance the attachment and migration of human osteoblasts in three dimensional culture . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Experiment 1 revealed that IGF 1 ( P < 0 . 001 ) , type 1 ( P=0 . 008 ) and 2 ( P=0 . 005 ) IGF Rs and IGFBP 5 ( P < 0 . 05 ) mRNA levels were significantly elevated in early regressing CL . ^^^ Abrogation of LH action following GnRH antagonist administration ( Experiment 2 ) resulted in a significant increase in expression for IGF 1 ( P < 0 . 001 ) , type 2 IGF R ( P=0 . 004 ) and IGFBP 5 ( P < 0 . 05 ) after only 12h , but these increases were transient . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
IGFBP 5 mutants with reduced affinity for IGF 1 ( N term ) or deficient in heparin binding ( HEP and C term E and F ) were also effective . ^^^ Thus IGFBP 5 can induce cell death by both sequestering IGF 1 and enhancing plasmin generation . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
The increased IGF binding protein 5 mRNA levels in fat and muscle suggest that IGF 1 signaling is increased in these tissues in C3H mice . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
Parallel application of IGFBP 3 had borderline inhibitory effects while the addition of 40ng / mL of IGFBP 5 enhanced the chemotactic effect of IGF 1 on MPC . ^^^ In conclusion , our results show that IGF 1 and 2 are chemotactic factors for MPC and indicate that IGFBP 5 both modulates the IGF 1 effect and directly stimulates migration of human mesenchymal progenitor cells . . ^^^
Interacting proteins: P05019 and P24593	Pubmed	SVM Score :0.0
CONCLUSIONS : IGF 1 used in conjunction with IGFBP 5 , EGF , and vitronectin provides a superior alternative to serum for the rapid expansion and transplantation of cultured keratinocytes within the first week of treatment . ^^^