| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :1.0591576 |
| However , Val44Met IGF 1 binds IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and IGFBP 6 with equal affinity to IGF 1 , suggesting the maintenance of overall structure , particularly in the IGFBP binding domain . 1.0591576^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Enzymatic deglycosylation did not alter the high affinity of IGF binding protein 6 for IGF 2 ( Ka 4 . 4 + / 2 . 2 10 10 ( 11 ) M 1 ) or its preference for IGF 2 over IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we determined that IGF binding protein 6 ( IGFBP 6 ) , which sequesters IGF 1 , was a substrate for MMP processing . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Sheep fibroblasts produced seven IGFBPs : a 36 . 5 41 kDa protein induced in vitro by IGF 1 , likely representing oIGFBP 3 ; a 28 . 5 kDa protein that reacted with antisera to human IGFBP 2 , likely representing oIGFBP 2 ; 25 and 27 kDa proteins induced in fetal fibroblasts by IGF 1 ; a 22 kDa protein that was inhibited by IGF 1 , likely representing oIGFBP 4 ; and 21 and 23 kDa proteins labelled only by IGF 2 , suggesting their similarities to IGFBP 6 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The binding proteins IGFBP 1 and IGFBP 3 did not cross react , but both IGF 1 and IGF 2 markedly inhibited IGFBP 6 tracer binding to antiserum . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Treatment with IGF 1 , [ QAYL ] IGF 1 , or alpha IR 3 had no effect on steady state levels of the 2 . 5 kb IGFBP 3 or the 1 . 3 kb IGFBP 6 mRNA transcripts as measured by Northern blotting and quantitative autoradiography . ^^^ IGF 1 , forskolin , and the candidate growth factors had no effect on either IGFBP 3 or IGFBP 6 mRNA . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 6 ( IGFBP 6 ) is an O linked glycoprotein that binds insulin like growth factor 2 ( IGF 2 ) with marked preferential affinity over IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| In view of the relative abundance of IGFBP 4 , its relatively high affinity for IGF 1 and the low affinity of IGFBP 6 for IGF 1 , it is likely that the inhibitory effect of rat skeletal muscle derived IGFBPs on IGF 1 induced myogenesis is mediated principally by IGFBP 4 . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The levels of IGFBP mRNAs were not regulated by androgens or IGF 1 , but the level of IGFBP 6 mRNA was sharply increased by 1 , 25 dihydroxyvitamin D 3 ( 1 , 25 ( OH ) D 3 ) . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGF 1 , IGFBP 1 , and IGFBP 6 mRNAs were expressed at low levels . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 2 expression did not vary with IGF 1 or dexamethasone treatment in these cells ; IGFBP 1 mRNA was not detectable , and IGFBP 6 mRNA was low and inconsistent . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 3 and IGFBP 6 were used to study whether both IGF 1 and IGF 2 play a role in auto / paracrine stimulation of rat osteoblast growth . ^^^ Consistent with their IGF binding affinities , IGFBP 3 blocked both IGF 1 and IGF 2 stimulated DNA synthesis , whereas IGFBP 6 preferentially blocked IGF 2 stimulated DNA synthesis . ^^^ Because IGFBP 6 preferentially binds IGF 2 and is much less potent than IGFBP 3 in decreasing basal DNA synthesis in calvaria cells , IGF 1 but not IGF 2 appears to be an important auto / paracrine stimulator of DNA synthesis . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Enzymatic deglycosylation does not alter the high affinity of IGFBP 6 for IGF 2 ( Ka 4 . 4 + / 2 . 2 10 10 ( 11 ) M 1 ) or its preference for IGF 2 over IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 1 and IGFBP 6 are most sensitive to changes in IGF 2 structure , although IGFBP 1 binds IGF 1 and IGF 2 with equal affinity , whereas IGFBP 6 has a marked preferential binding affinity for IGF 2 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| RA enhanced IGFBP 6 gene expression by threefold in MDA MB 231 cells , whereas IGF 1 had no effect on IGFBP 6 gene expression in either cell line . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Competitive binding studies of rat and human IGFBP 6 with [ 125I ] IGF 2 and unlabeled IGF 2 or IGF 1 demonstrated that both IGFBPs bound IGF 2 with similar affinities ( Ka , 1 . 5 1 . 8 10 10 ( 11 ) M 1 ) and bound IGF 1 with approximately 25 to 35 fold lower affinity than IGF 2 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| To gain insight into the role of the insulin like growth factors ( IGFs ) in regulating lung development , we have used in situ hybridization histochemistry ( ISHH ) to examine the ontogeny and sites of expression of IGF 1 and IGF 2 , IGF binding proteins ( IGFBP 1 to IGFBP 6 ) , and IGF cell surface receptors in fetal rat lung from 15 to 21 days of gestation . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Previous work indicate that glucocorticoids inhibit the synthesis of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IG FBP 3 ) , 4 , and 5 , but not IGFBP 6 , in osteoblast cultures . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| To localize mRNAs for insulin like growth factor ( IGF ) 1 , IGF 1 receptor ( IGF IR ) , and IGF binding protein ( BP ) 1 to IGFBP 6 in the rat eye . ^^^ METHODS . cDNA sequences for IGF 1 , IGF IR , and IGFBP 1 to IGFBP 6 were used to synthesize 35S CTP labeled antisense and sense probes for in situ hybridization on 5 microns sections of the rat eye , including the retina , choroid , sclera , ciliary body , and cornea . ^^^ Using in situ hybridization , we have demonstrated mRNAs for IGF 1 , IGF IR , and IGFBP 2 to IGFBP 6 in specific histologic layers of the retina , choroid , ciliary body , and cornea in the rat . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| We now present evidence that PC 3 cells also produce IGFBP 6 in abundant quantity ; in the previous study this was apparently not detected in the IGF 1 bound fraction with the PEG precipitation and Western ligand blot assays . ^^^ In studies on IGFBP 6 actions , IGFBP 6 completely inhibited IGF 2 induced [ 3H ] thymidine incorporation in MC3T3 E 1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF 1 induced [ 3H ] thymidine incorporation . ^^^ This differential effect is associated with the fact that IGFBP 6 has greater affinity for IGF 2 than IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Northern blots showed readily detectable transcripts for IGF 1 ( 6 . 7 and 0 . 9 kb ) , IGFBP 2 ( 1 . 8 kb ) , IGFBP 3 ( 2 . 8 kb ) , IGFBP 4 ( 2 . 6 kb ) , and IGFBP 5 ( 6 . 0 kb ) , but not for IGFBP 6 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGF 1 , size decrease when treated with O glycanase , but not N glycanase , reactivity with IGFBP 6 antiserum , presence of a 1 . 3 kilobase pair mRNA that hybridized to IGFBP 6 specific complementary DNA , and N terminal amino acid sequence corresponding to IGFBP 6 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Both treatments increase serum ALS , IGFBP 3 , and IGF 1 levels , and decrease IGFBP 2 , whereas IGFBP 6 is unaffected . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| At high cell density and with high concentrations of IGF 1 , des ( 1 3 ) IGF 1 ( 40 ng / ml ) or IGF 2 ( 80 ng / ml ) , the mitogenic activities of the IGFs diminished concomitantly with the appearance in the culture medium of an additional IGFBP identified as IGFBP 6 , whose production depended on activation of the type 1 IGF receptor . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : These results indicate a coordinate gene expression of the IGF system in microembolised porcine myocardium , compatible with a role of IGF 1 , IGFBP 3 , IGFBP 5 , and IGFBP 6 in inflammation linked angiogenesis and / or repair processes . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Fasting levels of IGF 1 , free IGF 1 , IGF 2 , IGFBP 3 , and insulin increased significantly within 3 months of GH treatment , whereas IGFBP 1 , IGFBP 2 , and IGFBP 6 showed no change . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| However , the rather limited data have been inconclusive so far and plasma concentrations of both insulin like growth factor 1 ( IGF 1 ) and binding proteins ( IGFBP 1 to IGFBP 6 ) have not been measured simultaneously in depressed patients . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGF 1 had similar effects on IGFBP 6 protein and mRNA levels . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Analyses of the cell cycle ( flux cytofluorometry ) , mitogenic activity ( radiolabeled thymidine incorporation ) , and the number of viable cells ( 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide test ) showed that the mitogenic effects of serum , IGF 1 , IGF 2 , and des ( 1 3 ) IGF 1 , a truncated IGF 1 analogue with no affinity for IGFBP 6 , were depressed in both transfected cell lines . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The combination of retinoic acid plus IGF 1 had no additional effect on IGFBP 6 or 2 above that observed with retinoic acid alone , whereas IGF 1 together with retinoic acid attenuated the decrease in IGFBP 3 observed with retinoic acid alone . ^^^ The decrease in IGFBP 6 was less marked when cells were treated with a combination of IGF 1 and forskolin . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Whereas 6 6 5 , like IGFBP 6 , had a marked preference for binary complex formation with IGF 2 rather than IGF 1 , it formed ternary complexes more efficiently with IGF 1 , like IGF BP 5 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : Lower serum concentrations of IGFBP 6 , 3 and 1 , but higher PSA concentrations were seen in the breast cancer group , and collectively these would suggest that there is an increase in bioavailable IGF 1 in breast cancer . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 6 ( IGFBP 6 ) differs from IGFBPs 1 5 in that it binds IGF 2 with marked preferential affinity over IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 6 differs from other IGFBPs in having the highest affinity for IGF 2 and in binding IGF 1 with 20 100 fold lower affinity . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| To enhance our understanding of the actions of growth factors on the IGF 2 axis in bone , we tested the effects of TGF beta 1 , basic FGF , PDGF BB , IGF 1 , and IGF 2 on the expression of IGFBP 6 in cultures of osteoblast enriched cells from 22 day fetal rat calvariae ( Ob cells ) . ^^^ In contrast , basic FGF , PDGF BB , IGF 1 , and IGF 2 did not change IGFBP 6 mRNA levels in Ob cells . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| A distinctive property of IGFBP 6 is its preferential affinity for IGF 2 relative to IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Patient serum contained low levels of IGF 1 , IGFBP 3 and ALS , while the concentrations of IGFBP 2 and IGFBP 6 were markedly elevated . ^^^ Northern blot analysis of total mRNA isolated from the tumour demonstrated high expression of the IGF 2 gene and abundant 1 . 1 kb IGFBP 6 transcript , while the genes encoding IGFBP 3 , 4 and 5 were only weakly expressed and mRNA of IGFBP 1 , 2 and IGF 1 could not be detected . mRNAs for the IGF type 2 receptor could be easily demonstrated , whereas those for the insulin and IGF type 1 receptor were hardly detectable . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Upon withdrawal of serum , 30 nM IGFBP 6 also decreased apoptosis ( within 4 h ) and increased protein content and sodium dependent phosphate uptake ( within 24 h ) , but less potently than IGF 1 . ( 125 ) 1 labeled rhIGFBP 6 did not bind to the cells , and cold IGFBP 6 did not affect ( 125 ) 1 labeled IGF 1 binding . ^^^ Production of IGF 1 , IGF 2 , and IGFBP 6 by the cells or significant degradation of rhIGFBP 6 could not be detected within 24 h of incubation . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Because the anatomical distribution of IGFBPs is likely to dictate IGF bioavailability , we determined the cellular distribution and expression of IGF 1 , IGF 2 , and IGFBP 1 to IGFBP 6 in epiphyseal growth plates of the fetal sheep , using immunocytochemistry and in situ hybridization . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| In addition , 12 pairs of significantly coexpressed IGF system components were detected ( IGF 1 < > IGF 1R , IGF 1 < > IGF 2R , IGF 1 < > IGFBP 3 , IGF 1 < > IGFBP 6 , IGFBP 3 < > IGF 1R , IGFBP 6 < > IGF 1R , IGFBP 1 < > IGF 2R , IGFBP 3 < > IGF 2R , IGFBP 5 < > IGF 2R , IGFBP 3 < > IGFBP 5 , IGFBP 3 < > IGFBP 6 , IGFBP 5 < > IGFBP 6 ) . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| This study evaluated the regulation of IGFBPs in human aorta smooth muscle cells by cyclic AMP , dexamethasone and IGF 1 . cAMP decreased IGFBP 3 , increased IGFBP 4 and increased IGFBP 6 . ^^^ IGF 1 increased IGFBP 3 and IGFBP 6 while decreasing IGFBP 4 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The insulin like growth factor ( IGF ) system includes IGF 1 and IGF 2 , the type 1 and type 2 IGF receptors , and specific IGF binding proteins ( IGFBP 1 to IGFBP 6 ) . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| RESULTS : IGF 1 , ICGF 2 , IGF 1R , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 , and IGFBP 6 were expressed by LAM cells . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : : Our findings are consistent with the hypothesis that IGFBP 6 inhibits cell growth by binding to endogenously produced IGF 2 , thereby preventing IGF 2 from interacting with the IGF 1 receptor to stimulate cellular proliferation by an autocrine mechanism . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGF 1 bioactivity is regulated in part by its six binding proteins ( IGFBP 1 to IGFBP 6 ) ; insulin inhibits the production of IGFBP 1 and perhaps IGFBP 2 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 6 ( IGFBP 6 ) is a high affinity IGFBP with substantially greater affinity for insulin like growth factor 2 ( IGF 2 ) than IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Intact IGFBP 1 , IGFBP 2 and IGFBP 6 inhibited in equimolar concentration the IGF 1 and IGF 2 stimulated DNA synthesis and cell proliferation , whereas the biological activity of IGFBP 3 was complex . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Compared with controls , immunoreactivity in the spinal motor neurons of patients with amyotrophic lateral sclerosis was 64 % higher for IGFBP 2 , 46 % higher for IGFBP 5 , and 33 % higher for IGFBP 6 , with upregulation of IGF 1 receptors . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^ When VA deficient rats received a single injection of retinoic acid ( 2 mg / rat ) , tissue IGF 1 and IGF IR gene expression did not change after 4 or 8 h , while the expression of IGF 2 , IGFBP 4 , and IGFBP 6 mRNAs in some tissues increased rapidly . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| After binding with IGFBP 1 , IGFBP 2 and IGFBP 6 , IGF 1 passes through epithelium and reaches the target cells . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 6 were examined by specific assays . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| When added exogenously , IGFBP 6 reduced O2A cell survival in the absence of IGF 1 and inhibited IGF 1 stimulated survival in a partially IGF 1 dependent and partially IGF 1 independent fashion . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The genes HOXC 4 , SP 1 and IGFBP 6 were localized to band q 21 , COL2A1 was localized to bands q 21 q23 , IGF 1 was localized to band q 26 , MB to band q 31 and the gene CYP2D6 was localized to band q 35 . ^^^ The cytogenetic assignment of SP 1 , IGFBP 6 , COL2A1 , IGF 1 , MB and CYP2D6 is first reported here and the assignment of HOXC 4 refines the previous assignment of this gene . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 6 had virtually no inhibitory effects on IGF 1 , but was moderately effective against IGF 2 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Similar to IGF 1 , IGFBP 6 mRNA expression showed little variation with follicle size . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Within the family of six binding proteins , IGFBP 6 is unique in having a 20 100 fold higher affinity for IGF 2 over IGF 1 and appears to act primarily as an inhibitor of IGF 2 actions . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 6 is unique in its 20 100 fold IGF 2 binding specificity over IGF 1 . ^^^ The N domain has rapid association kinetics , similar to full length IGFBP 6 , but both IGF 1 and 2 dissociate rapidly from this domain , thereby reducing its binding affinity for IGF 2 approximately 50 fold . ^^^ However , the N domain binds IGF 1 and 2 with similar affinities and it has a similar IGF 1 binding affinity to full length IGFBP 6 . ^^^ This suggests that the C domain confers the IGF 2 binding preference of IGFBP 6 ; indeed , IGF 1 bound inconsistently with very low affinity to the C domain . ^^^ The results of the binding studies are supported by the effects of the IGFBP 6 domains on IGF induced colon cancer cell proliferation ; the N domain inhibited IGF 2 induced proliferation with approximately 20 fold lower potency than IGFBP 6 and it was equipotent in inhibiting IGF 1 and IGF 2 induced proliferation . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| In contrast , initiated progenitors did not exit the cell cycle when stimulated with PDGF and / or FGF 2 . cDNA oligoarray analysis and RT PCR verification showed an early upregulation / induction of growth factor / receptors , PDGF A , PDGFR beta , IGFR 1 , IGF 1 and 2 , IL 6 , MEGF 5 , FRAG 1 , IRS 2 , HSPG , and FGFR 1 , followed by a late increase in the expression IGFBP 6 , PDGF alpha , FGFR 4A , c / ERB A , and FGFR 4 , 2 , and 1 during the tumorigenic progression . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| However , ES did not significantly alter the transcription of igf 1 , igfbp 2 , igfbp 5 and igfbp 6 genes . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| In all subjects we assessed plasma levels of ghrelin , leptin , soluble leptin receptor , insulin , IGF 1 , IGFBP 1 , IGFBP 3 and IGFBP 6 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| On the other hand , exogenous IGF 1 induced 8 11 % ( p < 0 . 05 ) increases in the proliferation , but cyclosporin A induced 30 80 % ( p < 0 . 05 0 . 01 ) reductions in the mRNA expression levels for endogenous IGF 1 , IGFR 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Clear cell RCC were characterized by significant increases in the mRNA expression of IGF 1 , IGFBP 3 and IGFBP 6 while papillary RCC exhibited down regulated expression of IGF 1 , IGFBP 4 and IGFBP 5 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Igfbp 6 is a secreted factor that specifically binds insulin like growth factor 2 ( IGF 2 ) , prevents its binding to the IGF 1 receptor , and is thought to regulate the activity of IGF 2 in growth and differentiation . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| IGFBP 6 mRNA was detected by Northern blot analysis in both cell types but was not regulated by IGF 1 . ^^^ In BME cells , IGFBP 6 protein levels were readily detectable under basal conditions and were increased by IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| RESULTS : IGF 1 and IGFBP 4 concentrations were lower whereas the concentration of IGFBP 6 was higher in synovial fluids from patients with prosthesis loosening than in synovial fluid from patients with osteoarthrosis . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The IGF system is a complex network comprised of two growth factors ( IGF 1 and 2 ) , cell surface receptors ( IGF IR and IIR ) , six specific high affinity binding proteins ( IGFBP 1 to IGFBP 6 ) , IGFBP proteases as well as several other IGFBP interacting molecules , which regulate and propagate IGF actions in several tissues . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| Plasma concentrations of GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 did not demonstrate any significant differences between treatment groups and controls . ^^^ Changes in the systemic osteotropic growth factors GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 do not seem to be of importance during distraction osteogenesis . . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| The 31 , 000 Mr protein was identified as IGF BP 2 by specific immunoreactivity with two polyclonal antibodies , and the 24 , 000 21 , 500 Mr doublet was identified as IGF BP 6 by reactivity with a specific polyclonal antibody and by marked preferential affinity for IGF 2 over IGF 1 by ligand blotting . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| This probably reflected the differential affinities of the endogenous IGF BPs ( IGF BP 6 and 2 ) for IGF II / IGF 1 . ^^^ |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24592 and P05019 |
Pubmed |
SVM Score :0.0 |
| NA |
|