Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.71244273
In contrast , addition of IGFBP 4 ( 500 ng / ml ) to human fibroblast cultures with IGF 1 ( 20 ng / ml ) almost completely inhibited the stimulatory effect of IGF 1 on DNA synthesis and no increase in fragment was detected in the CM . 0.71244273^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Dihydroxyvitamin D 3 differentially regulates the production of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 4 in mouse osteoblasts . 1 , 25 Dihydroxyvitamin D 3 [ 1 , 25 ( OH ) 2D3 ] induces differentiation and inhibits proliferation in many cell types including bone cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In this study we have exploited the high affinity and specificity of IGF binding protein 4 ( IGF BP 4 ) and IGF BP 5 for IGF 1 and IGF 2 to determine whether these growth factors are involved in the nerve sprouting reaction in paralyzed skeletal muscle . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In the present study we have investigated the effect of TNF alpha on the secretion of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 4 ( IGFBP 4 ) by clonal mouse osteoblasts ( MC3T3 E 1 cells ) using subconfluent in vitro cultures and serum free conditions . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) enhanced proteolysis of IGF binding protein 4 in conditioned medium from primary cultures of human decidua : independence from IGF receptor binding . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : Xanthine / xanthine oxidase and H2O2 stimulated increases in IGF 1 mRNA and protein levels and reduced IGF binding protein 4 levels in conditioned medium . ^^^ CONCLUSION : Reactive oxygen species increased vascular smooth muscle cell synthesis of IGF 1 and reduced levels of the inhibitory IGF binding protein 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Mucosal mRNA expression of IGF 1 and IGF binding protein 4 ( IGFBP 4 ) was evaluated by northern blot analysis using rat cDNA probes . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The stimulatory effect of activin was also blocked in a dose dependent manner by added IGF binding protein 4 or 5 , and the effects were reversed by IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In a previous study , insulin like growth factor 1 ( IGF 1 ) and a potential inhibitory binding protein , IGF binding protein 4 ( IGFBP 4 ) , were found to be expressed in a cell specific and temporally dynamic manner in the mouse uterus during the periimplantation period . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF binding protein 4 ( IGFBP 4 ) inhibits IGF 1 action in vitro and is the most abundant IGFBP in the rodent arterial wall . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Total ribonucleic acid ( RNA ) was isolated from muscle biopsy samples , and ribonuclease protection assays were performed using human complementary DNA clones for IGF 1 , IGF binding protein 4 , myosin , and actin . ^^^ Ten weeks after Lupron treatment , messenger RNA ( mRNA ) concentrations of IGF 1 decreased significantly , whereas there was a trend toward higher IGF binding protein 4 concentrations , with no change in myosin or actin mRNA concentrations . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In the current study , we examined the expression of IGF binding protein 4 ( IGFBP 4 ) , IGF 1 receptor ( IGF IR ) , and IGF 1 in the mouse uterus throughout the estrous cycle . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Northern blot analysis , in situ hybridization , and immunohistochemical studies of these `` early ' ' lesions demonstrated increased expressions of IGF 1 and IGF binding protein 4 ( IGFBP 4 ) in altered parenchymal cells , and a decreased expression of IGFBP 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The 24 kDa IGF binding protein representing IGF binding protein 4 was down regulated in all IGF 1 treated animals , but the decrease was more marked in UN animals . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Intact IGF binding protein 4 and 5 and their respective fragments isolated from chronic renal failure serum differentially modulate IGF 1 actions in cultured growth plate chondrocytes . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Plasma GH levels were unchanged during the entire course of the study , but a decrease in serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and IGF binding protein 4 ( IGFBP 4 ) occurred after 10 , 30 , and 48 days . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Pregnancy associated plasma protein A ( PAPP A ) is an IGF binding protein 4 ( IGFBP 4 ) metalloproteinase that cleaves inhibitory IGFBP 4 to amplify local IGF 1 bioavailability in vitro . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
A single RE bout resulted in increased levels of mRNA for IGF binding protein 4 ( 84 % ) , MyoD ( 83 % ) , myogenin ( approximately 3 fold ) , cyclin D 1 ( 50 % ) , and p 21 Waf1 ( 16 fold ) , and a transient decrease in IGF 1 mRNA ( 46 % ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Both GH and IGF 1 stimulated the accumulation of IGF 1 receptor mRNA in the most immature proliferative zone cells but did not alter the accumulation of IGF binding protein 4 mRNA in any fraction . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Pregnancy associated plasma protein A ( PAPP A ) is an IGF binding protein 4 protease that can function to increase local IGF 1 bioavailability . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In this study , we sought to determine by Western ligand blotting the effects of growth hormone , IGF 1 and IGF 2 on the production of IGFBP 3 and IGFBP 4 in TE 89 human osteosarcoma cells and in untransformed normal human bone cells derived from rib . ^^^ IGF 1 and IGF 2 , which increased human bone cell proliferation , decreased the level of IGFBP 4 ( 30 % of control at 100 micrograms / l IGF 1 and IGF 2 ) but increased the level of IGFBP 3 ( 3 10 fold at 100 micrograms / l IGF 1 and IGF 2 ) after 48 h of treatment in the conditioned medium of both low and high density TE 89 cell cultures . ^^^ Similar changes in IGFBP 3 and IGFBP 4 levels were also seen in the conditioned medium of human bone cells derived from rib after treatment with IGF 1 and IGF 2 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , IGFBP 4 levels were diminished 54 % and 73 % by 100 ng / ml IGF 1 and IGF 2 , respectively , with no response to insulin . ^^^ IGF 1 , IGF 2 , and insulin all stimulated an approximately 50 % increase in IGFBP 4 concentrations . ^^^ To test the hypothesis that IGF induced alterations in IGFBP 3 and IGFBP 4 concentrations were regulated via the type 1 IGF receptor , we attempted to block IGFBP changes with type 1 IGF receptor antibody alpha IR 3 and to induce IGFBP changes with an IGF 2 analog , [ Leu 27 ] IGF 2 , with little affinity for the type 1 receptor . alpha IR 3 failed to block either the IGF induced rise in IGFBP 3 in each cell line or the decline in IGFBP 4 in N 3652 CM . [ Leu 27 ] IGF 2 was as potent as IGF 2 or IGF 1 in inducing changes in IGFBP 3 and IGFBP 4 concentrations . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The levels of IGFBP 4 and IGFBP 5 were increased in MCF 7 cells by estradiol and IGF 1 , respectively , indicating that these BPs may contribute to the growth stimulatory response to these mitogens . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 and 2 caused a minimal ( less than 43 % ) increase in IGFBP 5 mRNA abundance and had no effect on IGFBP 4 mRNA abundance . ^^^ IGF 1 and 2 ( 100 ng / ml ) stimulated 6 8 fold increases in IGFBP 5 levels , whereas IGFBP 4 was inhibited . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Perfused IGFBP 1 , 2 , 3 , and IGF 1 also crossed the capillary boundary but in contrast to IGFBP 4 , preferentially localized in cardiac muscle with a connective tissue / muscle ratio of approximately 1 : 3 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
By inhibiting IGF 1 production and specifically inducing biosynthesis of the inhibitory binding protein IGFBP 4 , PDGF may set in motion mechanisms to limit the final magnitude of the mitogenic response . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To determine whether the effects of IGFs on IGFBP 4 might be exerted through alterations in IGFBP 4 degradation , we incubated cell free , fibroblast conditioned media from either sheep or human dermal fibroblasts with or without IGF 1 , IGF 2 ( each 1 microgram / ml ) , or insulin ( 10 micrograms / ml ) for 72 h at 37 C . ^^^ Similarly , incubation of cell free conditioned media containing recombinant human IGFBP 4 with IGF 1 caused a reduction in detectable levels of the 28K protein . ^^^ Alternatively , binding of IGF 1 or 2 to IGFBP 4 may enhance the susceptibility of IGFBP 4 to proteolytic degradation . ^^^ The demonstration that IGF 1 and IGF 2 can promote directly the proteolytic degradation of IGFBP 4 into fragments that do not bind IGFs provides a novel mechanism by which the IGFs may increase their own availability and / or activity in biological fluids . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Previous work indicated that hIGFBP 4 is the predominant IGFBP expressed by human osteoblast like cells , and that IGFBP 4 binds and inhibits the mitogenic activities of IGF 1 and IGF 2 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Purified IGFBP 4 inhibited the binding of [ 125I ] IGF 1 by its receptor and blunted stimulation of [ 3H ] thymidine incorporation by IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 and 3 enhanced IGF 1 stimulation of DNA synthesis in MCF 7 cells while IGFBP 4 and 5 had no effect . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The decreased synthesis of the autocrine / paracrine growth factor IGF 1 together with decreased levels of a positive modulator IGFBP 3 , and the increased levels of a negative modulator IGFBP 4 in the extracellular milieu , may be responsible for the reduced proliferative capacity in cells expressing abundant connexin43 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and its binding protein IGFBP 4 in human prostatic hyperplastic tissue : gene expression and its cellular localization . ^^^ To add information on the mechanism of IGF 1 action in the human prostate , we studied the expression and cellular localization of mRNA encoding IGF 1 and IGFBP 4 in human prostatic hyperplastic ( BPH ) tissue . ^^^ Northern analysis of total RNA extracted from BPH tissues with cDNA probes containing the entire coding regions for IGF 1 and IGFBP 4 documented the presence of multiple IGF 1 mRNA transcripts with lengths of 7 . 5 , 1 . 7 , 1 . 3 , and 1 . 1 kilobases and a single 2 . 1 kilobase transcript of IGFBP 4 mRNA . ^^^ In situ hybridization with the cDNA probes used for Northern analysis and with cRNA probes synthesized from the respective cDNA demonstrated that IGF 1 mRNA was only localized in the stromal cells , whereas IGFBP 4 mRNA was predominantly expressed by epithelial cells . ^^^ Our results suggest that in the human prostate , the locally secreted IGF 1 exerts its principal biological effects with a paracrine mode of action and demonstrate that IGFBP 4 is mainly expressed by IGF 1 target cells . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 and IGF 2 but not insulin evoked a marked decrease of IGFBP 4 as early as 4 hours after treatment . ^^^ IGFBP 4 mRNA abundance , however , was entirely unaffected by IGF 1 for up to 48 hours . ^^^ IGF 1 analogues with high affinity for the IGF 1 receptor and weak affinity for IGFBP paradoxically evoked a small increase in IGFBP 4 , probably through a general increase in protein synthesis . ^^^ IGF 1 only minimally decreased IGFBP 4 content in medium of sparse cultures , whereas it completely abolished IGFBP 4 content in conditioned medium of superconfluent SMCs . ^^^ Addition of IGF 1 to cell free medium conditioned by confluent , but not by sparsely cultured , SMCs led to rapid degradation of IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Both IGF 1 and IGF 2 were capable of inducing a decrease in IGFBP 4 ; however , IGF 2 was more effective . ^^^ To assess the biologic consequences of IGF 2 induced IGFBP 4 proteolysis , we treated hOB with IGF 2 for 5 h , which decreased medium IGF BP 4 by 70 % , and then measured IGF 1 and insulin stimulation of [ 3H ] thymidine incorporation . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Although the proteolytically modified IGFBP 4 retained the ability to bind IGFs , the affinities were approximately 13 and 20 fold lower for IGF 1 and IGF 2 , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Basic FGF enhanced IGFBP 2 mRNA whether alone or in combination with IGF 1 and enhanced only IGFBP 4 mRNA when given alone . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 4 ( IGFBP 4 ) is an important regulator of insulin like growth factor 1 ( IGF 1 ) anabolic activity in bone . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , liver IGFBP 2 mRNA was much lower in amount than IGF 1 mRNA and IGFBP 4 mRNA and was sharply elevated by fasting , and decreased by refeeding . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Both IGFBP 4 and BP 6 accumulate during differentiation and increase further in response to IGF 1 or des ( 1 3 ) IGF 1 treatment . ^^^ In view of the relative abundance of IGFBP 4 , its relatively high affinity for IGF 1 and the low affinity of IGFBP 6 for IGF 1 , it is likely that the inhibitory effect of rat skeletal muscle derived IGFBPs on IGF 1 induced myogenesis is mediated principally by IGFBP 4 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
An IGFBP 4 / maltose binding protein fusion peptide expression in the pMal p 2 vector produced a fusion protein exhibiting both IGFBP immunoreactivity and IGF 1 binding activity with specificity characteristic of IGFBPs . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Western ligand blot analysis of conditioned medium showed that treatment of stromal cells with IGF 1 or IGF 2 induced a dose dependent reduction of detectable IGFBP 4 levels . ^^^ LR 3 IGF 1 , which has 1000 fold lower binding affinity than IGF 1 , increased detectable IGFBP 4 at all concentrations tested . ^^^ Des ( 1 3 ) IGF 1 , whose affinity for IGFBP 4 is 30 fold lower than that of IGF 1 , increased detectable IGFBP 4 at low concentrations ( 0 . 1 10 ng / mL ) , but reduced its levels at 100 ng / mL , consistent with the significant binding to IGFBP 4 at this concentration . ^^^ The addition of IGF 1 or IGF 2 to cell free conditioned medium from stromal cells cultured in the absence of IGFs resulted in the reduction of detectable endogenous IGFBP 4 levels . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Primary preadipocyte cultures expressed IGF 1 , IGFBP 2 , and IGFBP 4 messenger RNAs ( mRNAs ) , and conditioned medium ( CM ) contained IGFBP 3 [ approximately 46 , 000 mol wt ( M ( r ) ) ] , IGFBP 4 ( 24 , 000 M ( r ) ) , and a 30 , 000 M ( r ) IGFBP identified by immunoblot as IGFBP 2 . ^^^ Like primary cultures , undifferentiated 3T3 L 1 cells expressed IGFBP 4 mRNA , but insignificant levels of IGF 1 and IGFBP 2 mRNAs . 3T3 L 1 cell CM contained IGFBP 3 and IGFBP 4 , and with the addition of IGF 1 , a 30 , 000 M ( r ) IGFBP was also present . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The basal ( non stimulated ) and the IGF 1 stimulated growth of the S P cells was not significantly different from that of the C P cells , suggesting that overexpression of IGFBP 4 was not inhibitory to the growth of the HT 29 cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The effects of IGF 1 are modulated by several IGF binding proteins including IGFBP 3 , the main circulating carrier of IGF 1 , and IGFBP 4 , the main IGF binding protein produced by vascular smooth muscle cells in vitro . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In bovine fibroblasts , IGF 1 treatment induced IGFBP 3 and IGFBP 5 mRNA and protein secretion , and had a moderate effect on IGFBP 4 expression . ^^^ Basal IGFBP 4 expression was inhibited by dexamethasone , and this effect was counteracted by IGF 1 . ^^^ In human fibroblasts , IGF 1 treatment increased levels of IGFBP 3 and decreased levels of IGFBP 4 without influencing mRNA expression . ^^^ Dexamethasone alone decreased IGFBP 3 , IGFBP 4 , and IGFBP 5 mRNA , but it had no significant effect on IGFBP 3 , 4 , or 5 expression in the presence of IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 3 was stimulated by IGF 1 , IGFBP 4 by forskolin , and IGFBP 5 by IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 3 , and IGFBP 4 increased under r hGH and , in contrast to GH BP activity , remained high throughout the study . ^^^ In conclusion , in girls with Turner ' s syndrome , GH BP activity , IGF 1 , IGFBP 3 , and IGFBP 4 are induced by r hGH . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 4 ( IGFBP 4 ) is a 24 kDa protein that binds insulin like growth factor 1 ( IGF 1 ) and IGF 2 with high affinity and inhibits IGF action in vitro . ^^^ The 16 kDa IGFBP 4 fragment no longer inhibited IGF 1 stimulated thymidine uptake in vitro , suggesting that proteolytic processing of IGFBP 4 may have important functional consequences in vivo . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Minimal activity ( e . g . < 20 % of IGFBP 4 degraded in 24 h at 37 C ) was present in conditioned medium unless IGF 1 or IGF 2 was added . ^^^ As IGFBP 4 is a potent inhibitor of IGF action , and the activity of this protease is regulated by IGF exposure , the protease represents a novel system for regulating the actions of IGF 1 in this cell type . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
CONCLUSIONS : Experimental colitis in rats causes an increase in IGF 1 binding to the muscularis propria , which represents increased levels of IGFBP 4 and IGFBP 5 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
When added with IGF 2 , the protease resistant mutant IG FBPs 4 , but not wild type IGFBP 4 , suppressed IGF 2 enhancement of IGF 1 stimulated DNA synthesis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Recently , we showed that IGFBP 4 inhibited IGF 1 action and that the cells produced a protease that cleaved IGFBP 4 into non IGF binding fragments . ^^^ After the cleavage of IGFBP 4 , the cellular DNA synthesis response to IGF 1 was enhanced . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Treatment with IGF 1 or 2 consistently decreased IGFBP 4 levels in CM but tended to increase their mRNA levels under all situations . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Circulating IGF 1 is positively correlated with fetal weight ( r = 0 . 66 , P = 0 . 03 ) , circulating IGFBP 3 ( r = 0 . 54 , P = 0 . 01 ) and IGFBP 4 ( r = 0 . 52 , P = 0 . 01 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Studies on the molecular mechanisms for IGFBP 4 actions revealed that coincubation of bone cells with IGFBP 4 and 125I IGF 1 or 125I IGF 2 decreased the binding of both of these ligands in a dose dependent manner . ^^^ In addition , IGFBP 4 decreased the binding of IGF 1 tracer to purified type 1 IGF receptor . ^^^ These data in conjunction with data showing that IGFBP 4 had no effect on cell proliferation induced by analogs of IGF 1 or IGF 2 , which exhibited > 100 fold reduced affinity for binding to IGFBP 4 suggest that IGFBP 4 may inhibit IGF action by preventing the binding of ligand to its membrane receptor . ^^^ In contrast to IGFBP 4 , IGFBP 5 treatment increased the binding of IGF tracer to bone cells but did not increase the binding of 125I IGF 1 to type 1 IGF receptor . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In this study , we describe the mRNA expression of IGF 1 , IGF 2 , type 1 IGF receptor , IGFBP 2 , IGFBP 4 and IGFBP 5 during mouse lung development as studied by in situ hybridization techniques . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Messenger RNA for IGFBP 2 , 3 , and 5 were present , while IGF 1 and IGFBP 4 mRNAs were not detected . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Treatment of TM 3 cells with IGF 1 increased the levels of the 24 kDa IGFBP 4 , as well as 25 , 28 , 40 , and 44 kDa IGFBPs . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In the mature animal , serum IGF 1 levels were partially corrected and IGFBP 3 levels were normalized by GH , but no change could be induced in the reduced serum IGFBP 4 levels . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To determine if growth factors produced by MC3T3 E 1 cells have autocrine actions on these cells , the effects of IGF 1 , IGF 2 , TGF beta 1 , and IGFBP 4 on MC3T3 E 1 cell proliferation were determined . ^^^ Exogenous addition of IGF 1 and IGF 2 stimulated MC3T3 E 1 cell proliferation , but TGF beta 1 and IGFBP 4 inhibited MC3T3 E 1 cell proliferation . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Posttranslational regulation of IGFBP 4 may provide a means for cooperative control of local cell growth by IGF 1 and IGF II . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Pelvic leaflets were incubated in serum free medium for 18 h with effectors ( BSA , IGF 1 , IGF 2 , IGFBP 4 , or a combination of IGF and IGFBP 4 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 had a lesser effect , while IGFBP 2 had no effect on IGF 1 plus hCG induced testosterone formation . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 has approximately 1000 fold higher affinity than LR3IGF 1 towards IGFBP 3 , IGFBP 4 , total rat plasma IGFBPs and L 6 myoblast BP . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Messenger RNAs for IGF 1 , IGF 2 , and IGFBP 4 were detected by Northern analysis . ^^^ AGS cells expressed messenger ( mRNA ) RNAs for IGF 2 and IGFBP 4 but not for IGF 1 , whereas another gastric carcinoma cell line ( SIIA ) , whose growth is stimulated by IGF 1 , expressed mRNA IGF 2 but did not express mRNA for IGF 1 or IGFBP 4 : CONCLUSIONS : The relative absence of growth response of AGS cells to IGF 1 is due to the endogenously produced IGFBPs sequestering IGF 1 and preventing receptor interaction . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Interestingly , attachment of IGF 1 or 2 to IGFBP 4 results in enhancement of proteolysis , whereas attachment of either growth factor to IGFBP 5 results in inhibition of proteolytic cleavage . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Enhanced cell responsiveness to IGF 1 appears to be due to IGF 2 induced changes in pericellular IGFBP 3 and IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) is the local regulator of osteoblasts and one of its binding protein , insulin like growth factor binding protein 4 ( IGFBP 4 ) , binds to IGF 1 and suppresses its biological activity . ^^^ The stimulated DNA and collagen synthesis by IGF 1 or 17 beta estradiol were inhibited by IGFBP 4 in a concentration dependent manner . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , IGF 1 ( 10 ng / ml ) increased secretion of IGFBP 3 and 5 , whereas FSH ( 200 ng / ml ) decreased IGFBP 3 secretion and increased IGFBP 4 secretion ( p < 0 . 05 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Circulating levels of insulin like growth factor 1 ( IGF 1 ) , which is considered to be the local regulator of osteoblast activity and one of its binding protein , insulin like growth factor binding protein 4 ( IGFBP 4 ) which binds to IGF 1 and suppress its biological activity , were also measured . ^^^ The simplest explanation is that whereas the loss of bone depends upon the decreased level of IGF 1 after natural menopause , after surgical menopause it depends upon the increased level of IGFBP 4 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
An important role for IGF 1 in colonic adaptation after massive intestinal resection is indicated by 1 ) significantly enhanced colonic mucosal growth and water absorption with IGF 1 treatment and 2 ) postresection upregulation of colonic IGF 1 mRNA and alteration of IGFBP 3 and IGFBP 4 mRNA expression . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 changes were inversely correlated with IGF 1 variations . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Northern blotting confirmed that transcripts for IGFBP 4 as well as IGF 1 are expressed in thymic macrophages . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 4 ( IGFBP 4 ) , like the five other IGFBPs present in human serum , acts as a transport protein for insulin like growth factor 1 ( IGF 1 ) and IGF 2 and modulates their biological effects . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In hepatocytes , synthesis of IGFBP 1 , 2 , and 4 was observed ; insulin and IGF 1 decreased that of IGFBP 1 , and 2 while increasing that of IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , IGFBP 4 levels were diminished by increasing concentrations of IGFs in the CM of the NSCLCs A 549 , NCI H 157 , and U 1752 , with no response to insulin or the IGF 1 analog , whereas IGFBP 4 specific mRNA was not changed by IGF 1 or IGF 2 , as determined by Northern analysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Low concentrations of IGF 1 induced a marked loss of IGFBP 4 by Western ligand blotting ( WLB ) . ^^^ This effect was confirmed by the ability of media collected from cells exposed to increasing concentrations of IGF 1 to fragment recombinant IGFBP 4 , an effect blocked by EDTA . ^^^ This was confirmed by the ability of excess recombinant IGFBP 3 to inhibit the IGF 1 and Des [ 1 3 ] induced proteolysis of IGFBP 4 . ^^^ Addition of IGF 1 to media from cells unexposed to IGF induced IGFBP 4 proteolysis but this was not seen with LR 3 which does not bind to IGFBP 3 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased the abundance of IGFBP 3 , 4 and 5 in TC 1 conditioned medium ; this correlated with induction of IGFBP 3 mRNA , but not with that of IGFBP 4 or 5 mRNAs . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RNase protection assays using total RNA demonstrated that MC express all of the IGFBPs . [ 125I ] IGF 1 Western ligand blot of conditioned medium demonstrated that MC release IGFBPs of 24 , 29 , 32 kDa , and a doublet at 46 kDa , consistent with IGFBP 4 , 5 , 2 and 3 , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Treatment of cells with IGF 1 and IGF 2 resulted in a dose dependent increase of IGFBP 5 and a small increase in IGFBP 4 in conditioned media ( CM ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We previously found , using an IGF 1 affinity column for purification and a polyethylene glycol ( PEG ) precipitation assay for IGFBP detection , that PC 3 cells in culture produced a single predominant IGFBP , IGFBP 4 , which inhibits IGF activities . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 , itself , had little effect on the production of IGFBPs , but when added together with bFGF increased the levels of IGFBP 3 and IGFBP 4 by 12 . 4 + / 5 . 1 fold ( P < 0 . 05 ) and 27 . 4 + / 5 . 3 fold ( P < 0 . 02 ) , respectively . ^^^ The addition of purified rat IGFBP 4 to the cultures in the absence of added growth factors had no effect on the survival of dopaminergic neurons , but when added with IGF 1 potentiated the effect of IGF 1 on neuronal survival . ^^^ We propose that the up regulation of IGFBP 4 by IGF 1 and bFGF may serve to localize IGF 1 to sites of action in the nervous system and thereby potentiate the neurotrophic actions of IGF I . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Chondrocytes from osteoarthritic cartilage have increased expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) and 5 , but not IGF 2 or IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Northern blots showed readily detectable transcripts for IGF 1 ( 6 . 7 and 0 . 9 kb ) , IGFBP 2 ( 1 . 8 kb ) , IGFBP 3 ( 2 . 8 kb ) , IGFBP 4 ( 2 . 6 kb ) , and IGFBP 5 ( 6 . 0 kb ) , but not for IGFBP 6 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We investigated the expression of the mRNAs encoding IGF 1 , IGF 2 , the type 1 IGF receptor ( IGF T1R ) and two IGF binding proteins ( IGFBP 2 and IGFBP 4 ) in rat lung during the perinatum . ^^^ At day 20 of gestation IGF 1 , IGF T1R and IGFBP 4 mRNA levels were higher in lung than liver ( all P < 0 . 01 ) , whereas IGF 2 and IGFBP 2 mRNA levels were higher in liver than lung ( each P < 0 . 02 ) . ^^^ The expression of IGF 1 , IGFBP 2 and IGFBP 4 in lung was high before birth ( days 17 20f ) but decreased to low levels at days 21f , 22f or at birth ( 1d ) but increased in the neonatal lung . ^^^ The patterns of expression of IGF 1 , IGF T1R , IGFBP 2 and IGFBP 4 , but not IGF 2 , in developing lung correspond to previously described phasic changes in lung cell proliferation rates . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Levels of IGFBP 1 were increased and IGFBP 3 , IGFBP 4 , IGF 1 , glucose and insulin were reduced at day 115 after undernutrition . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Recent studies demonstrate that IGF 2 in FFe is higher than in FFa ' whereas IGF 1 , IGFBP 3 and IGFBP 1 levels do not differ , underscoring the importance of local IGF 2 production by the granulosa and the importance of IGFBP 4 and IGFBP 2 in regulation of IGF 2 action within the follicle during its developmental pathway as an E 2 or A dominant follicle . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In subjects from 23 87 years , serum IGFBP 4 concentrations showed a significant positive correlation with serum PTH while serum IGFBP 5 concentrations showed a significant positive correlation with IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We also showed that IGFBP 4 inhibited IGF 1 induced estradiol release by GCs while proteolyzed IGFBP 4 did not . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) is the local regulator of osteoblasts and one of its binding proteins , insulin like growth factor binding protein 4 ( IGFBP 4 ) , binds to IGF 1 and suppresses biological activity . ^^^ IGFBP 4 proteolytic activity was stimulated by IGF 1 or IGF 2 added exogenously and was inhibited by EDTA or protease inhibitors . ^^^ IGFBP 4 proteolyzed in the conditioned medium from cells treated with PTH and 17 beta estradiol was less effective at inhibiting IGF 1 stimulated [ 3H ] thymidine incorporation into DNA compared with that proteolyzed in the conditioned medium from PTH treated cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In light of the high affinity of IGFBP 4 for IGF 1 , the abundant expression of IGFBP 4 in granulosa and theca cells of preantral and antral follicles of PCOS ovaries may lead to decreases in the bioavailability of IGF 1 in those follicles . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In summary , there is selective expression of IGF 1 , IGF 1 receptor , and IGFBP 2 and 4 mRNAs during the process of follicular selection in the gilt ovary , with IGFBP 4 expression being closely associated with follicular selection and luteinization . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In the human cells , IGF 1 increased media levels of IGFBP 3 through stimulation of IGFBP 3 mRNA and dissociation of cell bound IGFBP 3 , and decreased IGFBP 4 via potentiation of IGFBP 4 proteolysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 receptor , and five IGF binding proteins ( IGFBPs ) , IGFBP 1 , IGFBP 2 , IGFBP 3 , IGFBP 4 , and IGFBP 5 in smooth muscle cells ( SMCs ) using colloidal gold immunocytochemistry . ^^^ Quantitative reverse transcription polymerase chain reaction ( QRT PCR ) performed on de novo atherectomy specimens identified mRNA for IGF 1 , IGF 1 receptor , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 levels and detected mRNA for IGFBP 3 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In conclusion , we report that EGF treatment for 4 weeks in Goettingen minipigs reduces circulating IGF 1 and IGFBP 3 , increases circulating IGFBP 1 , IGFBP 2 and IGFBP 4 , and induces a slight hyperthyroidism as judged from increased circulating levels of T3 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In conclusion , significant correlations were observed between local changes in kidney IGF 1 and serum IGFBP 4 levels and the degree of renal ablation , suggesting a role for IGF 1 as a renotropic factor and , further , that IGFBP 4 is removed to a major extent through the kidney . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Thyroid hormone affects rat uterine expression of IGF 1 and IGFBP 4 . ^^^ In this study IGF 1 and IGFBP 4 mRNAs in uterus , oviduct and cervix from euthyroid , hypothyroid and T 3 treated rats were quantified by Northern blot analysis . ^^^ Our results demonstrate : 1 ) a marked decrease in IGF 1 and IGFBP 4 mRNA levels in the uterus but an increase in the oviduct of hypothyroid rats ; 2 ) a marked increase in IGF 1 and IGFBP 4 mRNA levels in the uterus but a net decrease in the cervix of T 3 treated rats . ^^^ The uterine changes in IGF 1 and IGFBP 4 mRNA levels associated with hypothyroid status were in agreement with those observed in liver . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
DNA synthesis appears to be in reciprocal relationship with hepatic expression of IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 4 , suggesting that IGF I / IGFBPs system is not involved in liver growth . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 , and IGFBP 4 and 5 mRNAs showed site specific tubular changes whilst remaining unchanged in other parts of the kidney normally expressing the genes : IGF 1 and IGFBP 4 mRNAs were reduced in TALs and proximal tubules respectively ; IGFBP 5 mRNA was reduced in most distal tubular cells but strongly expressed in a few of these cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Under basal conditions , SH SY5Y cells in serum free medium secreted IGF 2 , and traces of IGF 1 , IGFBP 2 and IGFBP 4 . ^^^ After 48 h , when IGFBP 2 and IGFBP 4 concentrations in the culture media had increased , des ( 1 3 ) IGF 1 remained the most active , but the activity of insulin now equalled or exceeded that of IGF 1 and IGF 2 . ^^^ This suggests a negative feedback mechanism involving partial sequestration of IGF 1 and IGF 2 by IGFBP 2 and IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
MK 801 inhibits the cortical increase in IGF 1 , IGFBP 2 and IGFBP 4 expression following trauma . ^^^ Cerebral contusions increase cortical expression of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 , mRNA levels increase at the contusion site ( IGF 1 , IGFBP 2 and 4 ) and along the ipsilateral cortex ( IGFBP 2 and 4 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Messenger RNA for IGF 1 , IGFBP 2 , and IGFBP 4 was determined by solution hybridization . ^^^ The IGF 1 mRNA did not change during the first 7 days and then decreased , and IGFBP 4 mRNA was increased transiently on day 7 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We determined whether the eicosanoid could regulate IGFBP 4 , a major form expressed by chondrocytes and , as such , act as a modifier of IGF 1 action at another level . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Later in the wounding response ( 7 14 dpl ) , the expression of IGFBP 4 and IGFBP 5 peaked within astrocytes and neurons , with IGFBP 5 mRNA being specifically localized to the glia limitans within the wound , suggesting an inhibitory role for these proteins , down regulating the effects of IGF 1 chronically . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Immunostaining of sections of normal and cancer tissue was negative for IGF 1 and IGF 2 ; IGFBP 2 was positive in 2 out of 10 cancer tissues and 7 out of 10 normal tissues ; IGFBP 3 was positive in 7 out of 10 cancer tissues and 7 out of 10 normal tissues ; and IGFBP 4 was positive in 5 out of 10 cancer tissues and 6 out of 10 normal tissues . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
CONCLUSION ( S ) : Follicles developing under the influence of exogenous androgens in ovaries in female to male trans sexuals appear to be similar to androgen dominant follicles in normo ovulatory women with regard to IGF 1 and IGF 2 levels , IGFBP profile , and the absence of IGFBP 4 protease activity . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 did not modulate the activity of the IGFBP 4 protease in solution but was able to prevent the inhibition seen with IGFBP 3 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
However , IGF 1 proved inhibitory to the accumulation of the 24 kDa IGFBP ( presumptive nonglycosylated IGFBP 4 ) ; no consistent effect was reported for the rat model . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Release of IGF 2 from IGF 2 IGFBP complexes after IGFBP 4 proteolysis by cell bound Pm was indicated by the observation that approximately 20 % of the 125I IGF 2 initially associated with endogenous IGFBP in reconstituted complexes was transferred to HT 29 D4 cell surface IGF 1 receptors . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
OP 1 alone or together with IGF 1 increased the steady state IGFBP 3 mRNA level and reduced the steady state mRNA levels of IGFBP 4 , 5 , and 6 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated mainly IGFBP 3 production , while EGF stimulated predominantly IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The results suggest that high glucose acts on pSMC to induce a change in IGFBP 4 proteolytic activity , which results in increased IGF 1 availability to its receptors thereby enhancing the SMC proliferative response . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The influence of IGF 1 on IGFBP 5 expression was specific because we found no evidence of changes in IGFBP 2 , IGFBP 4 , or cyclophilin expression . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 protein levels decreased in response to IGF 1 . ^^^ IGF 1 and TGF beta differentially regulate IGFBP 3 , IGFBP 4 , and IGFBP 5 expression , which , in turn , may modulate the in vitro and in vivo action of IGF I . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The rHGH independent rise in serum levels of the inhibitory binding protein IGFBP 4 suggests a mechanism by which improved bone formation is prevented despite successful elevation of IGF 1 and IGFBP 3 in the burned child . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
These IGFBPs , except the 24 kd form ( IGFBP 4 ) , were modulated by both IGF 1 and IGF 2 , with a maximum effect at 100 and 10 nmol / L , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We have previously shown that secretion of IGFBP 4 and IGFBP 5 by L 6 and BC3H 1 muscle cells was stimulated by treatment with either insulin , IGF 1 , or IGF 2 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
FSH stimulation of Inh alpha was attenuated by IGFBP 4 or 5 in a dose dependent fashion , and the effects were reversed by IGF 1 . ^^^ Anti IGF 1 antibody mimicked the inhibitory effects of IGFBP 4 and 5 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
C 2 BP4 myoblasts expressed sixfold higher levels of IGFBP 4 protein than C 2 LNL6 myoblasts . 125I IGF 1 cross linking indicated that IGFBP 4 overexpression reduced IGF access to the type 1 IGF receptor tenfold . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Since insulin like growth factor 1 ( IGF 1 ) is an important hormone regulator of intestinal proliferation , this study examined the effects of dietary protein content and tumor necrosis factor ( TNF ) on mRNA levels of IGF 1 , IGF 1 receptor , IGF binding protein 3 ( IGFBP 3 ) , and IGFBP 4 and on the histology of the colon , jejunum , and ileum in protein malnourished rats . ^^^ Simultaneous refeeding with the 20 % casein diet and administration of TNF led to a modest increase in IGF 1 and IGFBP 4 mRNA abundance in the colon . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Thus , although IGF 1 R mediates the autocrine / paracrine effects of the IGFs , IGF 1 R mRNA expression is most probably not involved in the differential IGFBP 2 / IGFBP 4 expression in leukemic cells . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 which is known to inhibit IGF 1 was negatively correlated with peak VO 2 leading , possibly , to increased IGF 1 bioactivity . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF binding proteins ( IGFBPs ) were detected in conditioned media from HSCs by 125I IGF 1 ligand blotting at apparent molecular masses of 24 and 41 to 45 kd that were immunologically identified as IGFBP 4 and 3 , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The mean serum IGFBP 4 level was increased by 25 % within 3 months after initiation of GH therapy and did not correlate with serum IGF 1 levels . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The degradation of IGFBP 4 was not stimulated when cells were incubated with the IGF 1 analogue , LR 3 IGF 1 , and insulin which binds to IGF 1 receptor but has little or no affinity for IGFBPs . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Previously , we have reported that porcine VSMCs synthesize and secrete IGF 1 and several forms of IGFBPs , including IGFBP 2 , IGFBP 4 , and IGFBP 5 . ^^^ In this study , we examined the role of autocrine / paracrine secreted IGF 1 in controlling the expression of IGFBP 4 and IGFBP 5 as well as the effects of these IGFBPs in modulating the cellular replication response to IGF 1 . ^^^ This increase was associated with a decrease in the activity of an IGF 1 regulated IGFBP 4 protease . ^^^ When added together with IGF 1 , exogenous IGFBP 4 inhibited IGF 1 induced DNA synthesis in a concentration dependent manner . ^^^ Therefore , IGFBP 4 and IGFBP 5 appear to be differentially regulated by autocrine / paracrine IGF 1 through distinct mechanisms . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Resection increased IGF 1 mRNA in both jejunum ( 183 % ) and ileum ( 249 % ) and up regulated IGFBP 4 mRNA levels in both tissues . ^^^ Increased levels of intestinal IGF 1 and IGFBP 4 mRNA suggest roles for IGF 1 and IGFBP 4 in mediating small bowel adaptation . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Large increases in serum levels of IGF 1 and IGFBP 5 and a transient increase in serum IGFBP 4 were found . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Anti TGF beta increased concentrations of IGF 1 5 . 8 fold and IGFBP 2 and IGFBP 3 by 8 and 7 fold in conditioned media whereas IGFBP 4 decreased 5 fold . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Estradiol increased serum IGF 1 , IGFBP 3 , and IGFBP 4 throughout the treatment period , but it did not influence other IGFBPs in serum . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Colony formation in soft agar was significantly inhibited up to 14 days after plating in the IGFBP 4 transfected cells when compared with the M 12 controls ( P < or = 0 . 01 ) : however , in the presence of des ( 1 3 ) IGF 1 , there was no significant difference between the control and IGFBP 4 transfectants in colony formation in soft agar . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The present results show that ovarian hormones and dietary protein content affect the plasma concentrations of IGF 1 , IGFBP 3 and IGFBP 4 and hepatic mRNA of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 4 in different manners . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 2 and 5 are found in the apical ectodermal ridge ( AER ) , and IGF 1 and IGFBP 4 in the region of the zone of polarizing activity ( ZPA ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Rats were treated with intravenous injections of 125I labeled IGF 1 , 125I IGFBP 3 , or 125I IGFBP 4 alone or with complexes of 125I IGF 1 and IGFBP 3 or IGFBP 4 . ^^^ The cellular localization of IGF and the IGFBP within the kidney was then determined . 125I IGF 1 , 125I IGFBP 4 , and 125I IGF I / IGFBP 4 complexes were found almost exclusively in vacuolar structures ( endosomes ) of proximal renal tubules . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Blocking the activity of IGFBP 4 did not significantly modulate the effect of IGF 1 induction of VEGF mRNA in HT 29 cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
As has been shown for other cell types , we found that IGFBP 4 had only inhibitory actions , inhibiting IGF 1 and IGF 2 stimulated proliferation and differentiation . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
When IGFBP 4 is added to monolayer cultures of cell types that do not secrete this protease , IGF 1 stimulation of DNA synthesis is significantly inhibited . ^^^ These studies were conducted to determine whether proteolysis of IGFBP 4 accounted for its reduced capacity to inhibit IGF 1 stimulated DNA synthesis . ^^^ The results show that cleavage of IGFBP 4 at lysine 120 , histidine 121 results in inactivation of the ability of IGFBP 4 to bind to IGF 1 . ^^^ Creation of a mutant form of IGFBP 4 that was not cleaved by the protease resulted in inhibition of IGF 1 stimulated actions . ^^^ The results suggest that IGFBP 4 can act as a potent inhibitor of the anabolic effects of IGF 1 and that the variables that regulate protease activity may indirectly regulate IGF 1 actions . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The injured sciatic nerve expressed IGF 1 and IGF 2 as well as IGFBP 4 and IGFBP 5 , whereas central nervous system ( CNS ) scar tissue expressed IGF 1 , IGFBP 2 , and IGFBP 5 . ^^^ In line with the in vivo findings , cultured Schwann cells expressed IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 5 mRNAs , whereas cultured astrocytes expressed IGF 1 , IGFBP 2 , and IGFBP 5 mRNAs . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Chronic LPS administration ( 250 microg / kg daily for 8 days ) significantly decreased body weight , serum levels of IGF 1 and pituitary GH content , whereas it increased circulating IGFBP 3 ( 47 kDa band ) , IGFBP 1 and IGFBP 2 ( 33 kDa band ) and the 24 kDa band ( which possibly corresponds to IGFBP 4 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To address this question , we performed a cross sectional study measuring serum levels of IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , IGFBP 4 and IGFBP 5 by specific immunoassays in 52 adults with Type 1 ( n=27 ) and Type 2 ( n=25 ) diabetes mellitus and 100 age and sex matched healthy blood donors . ^^^ IGF 1 correlated positively with IGFBP 3 and IGFBP 5 and negatively with IGFBP 1 and IGFBP 4 in all subjects . ^^^ Furthermore , biochemical markers indicating bone loss ( ICTP ) and increased bone turnover ( PTH , OSC ) correlated positively with IGFBP 1 and IGFBP 4 but negatively with IGF 1 , IGFBP 3 and IGFBP 5 , while the opposite was observed with bone formation markers ( PICP , B ALP ) and vitamin D 3 metabolites . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Cryostat sections of colon from TNB treated and control rats were hybridized with 35S labeled antisense probes for IGF 1 , IGFBP 3 , IGFBP 4 and IGFBP 5 . ^^^ Altered expression of IGFBP 3 , IGFBP 4 and IGFBP 5 in specific bowel layers during colitis suggests that they play a role in modulating IGF 1 action . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The mitogenic activity of exogenously added IGFs was reduced by the presence of IGF binding proteins of 24 , 30 , 34 , 41 and 45 kDa in supernatants of PLC cells identified as IGFBP 4 , 1 , 2 and 3 , respectively , by [ 125I ] IGF 1 ligand , immuno and northern blotting . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
C 6 cells produce IGFBP 3 , IGFBP 4 , a negligible amount of IGFBP 2 , and IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Release of insulin like growth factor binding protein 4 ( IGFBP 4 ) by tumour cells adjacent to bone may inhibit IGF 1 stimulated osteoblast growth and contribute to decreased bone formation . ^^^ IGFBP 4 may also be regulated post transcriptionally by a protease that is activated by IGF 1 or 2 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In AN , the levels of GH binding protein , free and total IGF 1 , free IGF 2 , and IGFBP 3 were significantly reduced ; total IGF 2 , IGFBP 2 , and IGFBP 4 levels were unchanged ; and IGFBP 1 was increased . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
This study represents the most comprehensive and detailed analysis of the physiology and anatomy of the mouse ovarian IGF system , and shows that 1 ) IGFBP 5 is linked to the survival of the slow growing and immature preantral follicles ; 2 ) IGF 1 is associated with the growth and survival of the rapidly growing large preantral and antral follicles ; 3 ) IGFBP 4 is an atretogenic candidate for mouse ovarian follicles ; 4 ) ovulatory doses of PMSG / hCG up regulate IGFBP 2 mRNA expression in the ovarian interstitium ; and 5 ) transcripts of IGF type 1 receptor and IGFBP 2 through 5 , but not those of IGF 1 are highly expressed in the mouse corpus luteum . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We conclude that the expression and time dependent production of IGFBP 3 , IGFBP 4 , and IGFBP 5 and their regulation by endogenous TGF beta 1 represent mechanisms by which human intestinal muscle cells regulate autocrine IGF 1 mediated growth . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The different effects of rhIGF 1 and its analogs on BMDM differentiation suggest that the accumulation of IGFBP 4 in BMDM development might have an inhibitory effect on IGF 1 actions by sequestering free IGF 1 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
CONCLUSION : GH significantly increased serum IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 4 and decreased serum IGFBP 1 and IGFBP 2 in post menopausal women . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated gene expression of IGFBP 2 and IGFBP 4 . ^^^ ANG 2 decreased IGF 1 , IGFBP 2 , and IGFBP 4 transcripts but increased the IGF 1 receptor transcript . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The addition of an excess of IGF 1 enhanced IGFBP 4 proteolytic degradation , whereas addition of IGFBP 2 or 3 or monoclonal antibodies against IGF 1 and 2 dose dependently inhibited IGFBP 4 proteolytic degradation . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased IGFBP 3 , IGFBP 2 and decreased IGFBP 4 , while TGF beta 1 decreased IGFBP 3 and apparently increased IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Regulation of IGF 1 , IGFBP 4 and IGFBP 5 gene expression by loading in mouse skeletal muscle . ^^^ Gene expression of IGF 1 , IGFBP 4 and IGFBP 5 was studied in hindhimb skeletal muscle of mice , which were either overloaded or unloaded for 8 days . ^^^ Overloading induced a 15 % hypertrophy in soleus muscle associated with a 60 % increase of IGF 1 transcript levels and a doubling of IGFBP 4 mRNA levels . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In addition , 5alpha DHT decreased IGFBP 4 mRNA and protein levels by 2 and 4 fold , respectively , and increased IGFBP 2 and 3 mRNA and protein levels by 6 and 7 fold ( for mRNA ) and 3 and 5 fold ( for protein ) , respectively . hFOB / AR 6 cells expressed the type 1 IGF receptor , but this was not regulated by 5alphaDHT . 5alphaDHT and IGFBP 3 specifically increased hFOB / AR 6 cell proliferation , and a monoclonal antibody specific for IGF 1 blocked this effect . ^^^ Thus , androgens increase the expression of IGF 1 , IGFBP 2 , and IGFBP 3 , but decrease levels of the inhibitory IGFBP 4 in an androgen responsive human osteoblastic cell line . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , IGF 1 or IGF 2 ( 6 . 5 nM ) preferentially induced the accumulation of IGFBP 3 ( 1 . 9 fold ) and to a lesser extent of IGFBP 4 , but did not show any effect on IGFBP 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 1 and IGFBP 4 acted in an inhibitory manner on IGF 1 induced DNA synthesis with calculated IC 50 values of 1 . 6 nM and 6 . 2 nM respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 is the major IGFBP produced by these cells when cultured in complete growth medium or in the presence of either IGF 1 or des ( 1 3 ) IGF 1 alone . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Circulating concentrations of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 , IGFBP 5 , 25 hydroxycholecalciferol ( 25 ( OH ) D 3 ) , and intact parathyroid hormone ( PTH ) were measured in 50 elderly women after sustaining a hip fracture and in 50 healthy age matched controls . ^^^ No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To test the hypothesis that impairment in bone formation in renal osteodystrophy in adults with chronic renal failure ( CRF ) might be mediated in part by alterations in circulating levels of the insulin like growth factor ( IGF ) system components , we compared serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 and IGFBP 5 in adults with CRF ( CRF patients with parathyroid hormone ( PTH ) < 100 pg / ml , PTH > 300 pg / ml and end stage renal failure ( ESRF ) patients ) versus age matched controls . ^^^ We found severalfold increased serum levels of IGFBP 3 ( 2 fold ) , IGFBP 4 ( 5 fold ) and slightly increased IGF 2 levels in ESRF patients as well as a 2 . 6 fold increase in free IGF 1 in CRF patients with PTH < 100 pg / ml . ^^^ This was most pronounced in CRF patients with PTH < 100 pg / ml showing also a significant increase in free IGF 1 and the lowest levels of the IGF inhibitory IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Local administration of IGF 1 over the right parietal bone significantly increased bone extract alkaline phosphatase activity ; this was completely blocked by an equimolar dose of intact IGFBP 4 , but not IGFBP 4 fragment . ^^^ A single sc administration of IGF 1 ( 2 microg / g BW ) significantly increased bone formation markers in both serum and skeletal extracts ; surprisingly , so did intact IGFBP 4 , but not fragment IGFBP 4 . ^^^ Subcutaneous administration of an equimolar dose of IGFBP 4 along with IGF 1 did not significantly block the IGF 1 effect . ^^^ Administration of intact IGFBP 4 significantly increased the serum 50 kDa IGF pool and decreased the 150 kDa IGF pool without significantly changing total IGF 1 . ^^^ This study demonstrates for the first time that a single local administration of IGFBP 4 inhibits IGF 1 induced increases in bone formation , whereas systemic administration of IGFBP 4 alone increases serum levels of bone formation markers . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In all subjects , free and total IGF 1 levels showed significant negative correlations with IGFBP 1 , IGFBP 2 , and IGFBP 4 ( that is , inhibitory IGF system components ) and significant positive correlations with IGFBP 3 and IGFBP 5 ( that is , stimulatory IGF system components ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Western ligand blotting showed induction of the IGFBP 3 doublet as well as IGFBPs with molecular masses of 24 kDa , most probably IGFBP 4 , by human GH , IGF 1 alone , and IGF 1 in combination with IGFBP 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The effects of IGF 1 are modulated by several IGF 1 binding proteins ( IGFBPs ) , including IGFBP 4 , the main IGFBP produced by vascular smooth muscle cells ( VSMCs ) . ^^^ In addition , we have demonstrated that IGF 1 and IGFBP 4 mRNA levels are upregulated in the hypertensive aorta of abdominally coarcted rats , a high renin hypertension model . ^^^ To obtain further insight into the IGF 1 system and to specifically study changes in IGFBP 4 , a known inhibitor of IGF 1 action , VSMCs were incubated with Ang 2 or thrombin . ^^^ These data suggest that downregulation of IGFBP 4 expression in VSMCs may play a critical role in vascular growth response to Ang 2 and thrombin in normal and diseased states , by increasing the bioavailability of IGF 1 for its cell surface receptor . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : Diabetes was associated with increased renal expression of IGFBP 1 mRNA ( diabetes 824+ / 236 vs control 264+ / 76 arbitrary units , P < 0 . 01 ) and decreased expression of mRNAs for IGF 1 ( diabetes 39+ / 7 vs control 185+ / 23 arbitrary units , P < 0 . 001 ) and IGFBP 4 ( diabetes 139+ / 25 vs control 383+ / 54 arbitrary units , P < 0 . 001 ) . ^^^ Aminoguanidine treatment inhibited the effects of diabetes on renal expression of mRNA for IGF 1 , IGFBP 1 and IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We examined the effects of IGF 1 on primary uterine myometrial cell proliferation , and on IGFBP 3 and IGFBP 4 gene expression . ^^^ A low dose of IGF 1 increased IGFBP 4 accumulation , and a high dose caused IGFBP 4 to disappear . ^^^ In cell free conditioned media IGF 1 protected IGFBP 3 and enhanced IGFBP 4 proteolysis . ^^^ Northern blot analysis indicated that IGF 1 increased IGFBP 4 mRNA accumulation by stabilizing the mRNA while IGFBP 3 gene expression was slightly decreased . ^^^ The results demonstrate that IGF 1 regulates IGFBP 4 post trancriptionally and post translationally , whereas IGFBP 3 is only affected post translationally . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Intact IGFBP 4 was decreased by IGF 1 , and the combination resulted in a similar reduction . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Effects of aging and caloric restriction on IGF 1 , IGF 1 receptor , IGFBP 3 and IGFBP 4 gene expression in the rat stomach and colon . ^^^ The purpose of this study was to determine the effects of aging and caloric restriction ( CR ) on insulin like growth factor 1 ( IGF 1 ) , IGF 1 receptor ( IGF IR ) , IGF binding protein 3 ( IGFBP 3 ) and IGFBP 4 expression in the stomach and colon of male Fischer 344 rats . ^^^ Stomach IGF 1 , IGFBP 3 and IGFBP 4 mRNA levels increased significantly ( P < / =0 . 05 ) , while colonic IGF 1 mRNA levels were unchanged in aged AL rats . ^^^ Increased expression of stomach IGF 1 , IGFBP 3 and IGFBP 4 in aged AL rats suggests that the stomach attempts to preserve IGF activity by increasing local expression of IGF 1 and IGFBPs . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In conclusion , the effect of stimulated endogenous GH with KP 102 administration increased plasma IGF 1 , 38 43 kDa IGFBP 3 and 24 kDa IGFBP 4 levels in the HI group of growing Holstein steers , but not in the LI one . ^^^ Thus , we strongly believe that the plasma IGF 1 and IGFBPs response to KP 102 treatment is modulated by the nutritional status of growing Holstein steers and the increased plasma IGF 1 concentration with KP 102 treatment may be regulated by plasma 38 43 kDa IGFBP 3 and 24 kDa IGFBP 4 in Holstein steers . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Human intestinal smooth muscle in culture produces insulin like growth factor ( IGF ) 1 and IGF binding protein ( IGFBP ) 3 , IGFBP 4 , and IGFBP 5 , which modulate the effects of IGF 1 . ^^^ R 3 IGF 1 , an agonist unaffected by IGFBPs , elicited concentration dependent increase in growth , measured by [ ( 3 ) H ] thymidine incorporation , and production of IGFBP 3 , IGFBP 4 , and IGFBP 5 , measured by Western blot . ^^^ Antagonists of the IGF 1 receptor , IGF 1 Analog or monoclonal antibody 1H7 , elicited concentration dependent inhibition of growth and decrease in IGFBP 3 , IGFBP 4 , and IGFBP 5 production , implying that endogenous IGF 1 stimulated growth and IGFBP production . ^^^ R 3 IGF 1 induced increase in IGFBP 3 , IGFBP 4 , and IGFBP 5 production was partially inhibited by a mitogen activated protein ( MAP ) kinase or a phosphatidylinositol 3 kinase ( PI 3 kinase ) inhibitor and abolished by the combination . ^^^ We conclude that endogenous IGF 1 stimulates growth and IGFBP 3 , IGFBP 4 , and IGFBP 5 production in human intestinal smooth muscle cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Plasmon resonance spectroscopy , ligand blot analysis , and saturation and displacement studies demonstrated a very low affinity of the C terminal IGFBP 4 fragment for the IGFs ( IGF 2 , K ( d ) = 690 nM ; IGF 1 , K ( d ) > 60 nM ) , whereas the N terminal fragment retained significant IGF binding properties ( IGF 2 , K ( d ) = 17 nM ; IGF 1 , K ( d ) = 5 nM ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 inhibited IGFBP 3 ( P < 0 . 01 ) , IGFBP 4 ( P < 0 . 01 ) , and IGF 1 mRNA expression , whereas at the protein level only IGFBP 3 was significantly decreased . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The expression of IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 , which serve as negative modulators of IGF 1 , was enhanced in CMA . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Serum IGF 1 , and its binding proteins ( IGFBP 3 , IGFBP 4 , and IGFBP 5 ) , as well as bone mineral density ( BMD ) at the lumbar spine , femoral neck , and radius midshaft were measured before and 1 year after antithyroid ( methimazole ) treatment . ^^^ Serum free thyroxine showed a positive correlation with serum levels of IGF 1 ( r = 0 . 73 , p < 0 . 05 ) , IGFBP 3 ( r = 0 . 59 , p < 0 . 05 ) , and IGFBP 4 ( r = 0 . 67 , p < 0 . 05 ) but not IGFBP 5 . ^^^ BMD at the radius midshaft was significantly lower in hyperthyroid patients at the start of the study and showed a positive correlation with serum IGF 1 ( r = 0 . 58 ; p < 0 . 001 ) and a negative correlation with IGFBP 4 ( r = 0 . 61 ; p < 0 . 05 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
An excess of IGF 1 enhanced IGFBP 4 degradation . ^^^ In contrast , IGFBP 2 or 3 or monoclonal antibodies against IGF 1 or 2 dose dependently inhibited IGFBP 4 degradation , and IGF 1 or 2 reversed this inhibition in a dose dependent manner . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
This study evaluated the regulation of IGFBPs in human aorta smooth muscle cells by cyclic AMP , dexamethasone and IGF 1 . cAMP decreased IGFBP 3 , increased IGFBP 4 and increased IGFBP 6 . ^^^ IGF 1 increased IGFBP 3 and IGFBP 6 while decreasing IGFBP 4 . ^^^ In cells incubated with cAMP and IGF 1 , media IGFBP 4 was decreased , despite increased IGFBP 4 mRNA , in this case secondary to the dominant effect of IGF 1 stimulated IGFBP 4 protease . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Compared with pair fed rats , zinc deficiency produced attenuated weight gain ( 43 % , P < 0 . 001 ) , lower serum IGF 1 and liver IGF 1 mRNA ( 52 % , P < 0 . 001 and 44 % , P < 0 . 05 ) , lower serum IGFBPs ( IGFBP 3 66 % , IGFBP 4 48 % , 34 29 kDa IGFBP cluster 53 % , P < 0 . 05 ) , lower liver GHR and its mRNA ( 20 and 34 % , P < 0 . 05 ) and lower serum growth hormone binding protein ( GHBP ) and its mRNA ( 56 and 48 % , P < 0 . 05 ; all comparisons vs PF rats ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Moreover , diabetic HREC cultures showed significantly less immunoreactivity for IGF 1 and for IGFBP 4 as compared to non diabetic HREC cultures . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
These include reductions in growth hormone , IGF 1 , IGFBP 3 , and IGFBP 5 and an increase in IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Treatment of confluent C 6 cells with 200 microg / ml Poly ( IC ) for 24 h decreased levels of immunoreactive IGF 1 in conditioned medium ( CM ) to 55 % of the control value , decreased IGF 1 receptor beta subunit levels to 28 % of the control value , and decreased levels of IGFBP 3 , IGFBP 4 , and IGFBP 5 protein in CM to 45 % , 50 % , and 30 % of the control values , respectively . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To eliminate possible actions of IGF 2 , if any was produced by osteoblasts derived from IGF 1 knockout mice , the IGFBP 5 effect was studied in the presence of exogenously added IGFBP 4 , a potent inhibitor of IGF 2 actions in bone cells . ^^^ Addition of IGFBP 4 blocked IGF 1 but not IGFBP 5 induced cell proliferation in osteoblasts derived from IGF 1 knockout mice . ^^^ In contrast to IGFBP 5 , local administration of IGFBP 4 had no significant effect on bone formation in C3H and IGF 1 KO mice . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Sepsis and bacterial lipopolysaccharide ( LPS ) injection decrease circulating concentrations of insulin like growth factor ( IGF ) 1 and induce an increase in IGFBP 1 and IGFBP 4 that may have impact upon IGF 1 anabolic actions . ^^^ In conclusion , our results show that IL 6 stimulates hepatic IGFBP 4 gene expression and production in vitro and in vivo , thereby suggesting another mechanism by which cytokines could control IGF 1 action . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : IGF 1 , ICGF 2 , IGF 1R , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 , and IGFBP 6 were expressed by LAM cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated thymidine incorporation and modified the pattern of IGFBPs , decreasing the inhibitory IGFBP 4 through down regulation of its mRNA , and increasing IGFBP 5 which , per se , was able to modulate HREC growth , exerting post transcriptional control . ^^^ The dose of IGF 1 that significantly decreased the IGFBP 4 / IGFBP 5 ratio was the same that stimulated HREC growth . ^^^ These results extend our previous observations in endothelial cells and suggest that the IGFBP 4 / IGFBP 5 ratio regulates IGF 1 induced growth of HRECs , whereas a general decrease in IGFBPs ( except for IGFBP 4 ) was the anti proliferative effect of chronic exposure to high glucose concentrations . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased the concentration of IGFBP 2 and 3 and decreased the concentration of IGFBP 4 in the medium of A 549 cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
No influence of T ( 3 ) on IGF 1 , IGF 2 , IGFBP 3 , or IGFBP 4 mRNA levels in hOB was observed . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Regulation of IGFBP 4 levels in human intestinal muscle by an IGF 1 activated , confluence dependent protease . ^^^ Human intestinal smooth muscle cells in culture produce insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 4 , and IGFBP 5 , which can modulate the effects of IGF 1 on growth . ^^^ This study examined the role of IGFBP 4 on IGF 1 induced growth and the mechanisms regulating IGFBP 4 levels . ^^^ IGFBP 4 inhibited IGF 1 induced [ ( 3 ) H ] thymidine incorporation . ^^^ IGFBP 4 mRNA levels were not altered by IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
GH treatment normalized serum IGF 1 in a majority of the patients and increased IGFBP 3 and 5 as well as IGFBP 4 and IGF 2 to values within normal range . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 proteolysis was inhibited by EDTA and 1 , 10 phenanthroline and was accentuated by the addition of IGF 1 and IGF 2 and , to a lesser extent , by des ( 1 3 ) IGF 1 . ^^^ Exposure of cells to PDGF BB for 48 hours resulted in an inhibition of IGFBP 4 proteolysis that was associated with a decrease in the concentration of IGF 1 in CM . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , CM from cells exposed to IL 1beta or TNF alpha incubated at 37 degrees C for 24 h did not show a significant decrease in IGFBP 4 abundance unless IGF 1 was present during the cell free incubation . ^^^ Addition of IGFBP 3 to aliquots of SFM CM reversed the IGF 1 mediated acceleration of IGFBP 4 proteolysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The effect of depolarization and N methyl D aspartate ( NMDA ) receptor blockade on insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) and IGFBP 4 expression was analysed in vivo . ^^^ No alterations in IGF 1 or IGFBP 4 mRNA levels were noted . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The insulin like growth factor binding protein 4 ( IGFBP 4 ) , the most abundant IGF binding protein produced by rodent smooth muscle cells ( SMC ) , is degraded by specific protease ( s ) potentially releasing IGF 1 for local bioactivity . ^^^ Myc tagged native and IGFBP 4 . 7A retained equivalent IGF 1 binding affinity . ^^^ Thus , IGFBP 4 . 7A results in greater growth inhibition than equivalent levels of native IGFBP 4 in vivo , demonstrating a role for IGFBP 4 proteolysis in the regulation of IGF 1 action . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Accordingly , successful antral follicle development , ovulation , and corpus luteum formation may be contingent on an IGFBP 4 deplete / PAPP A replete circumstance , hence resulting in an IGF 1 replete intrafollicular microenvironment . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We have previously shown that TGF beta 1 increased IGF 1 and IGF binding protein ( IGFBP ) 3 production in human bone marrow stromal ( hMS ) osteoblast progenitors and calcitriol stimulated IGFBP 3 and IGFBP 4 production . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : Thirty two tumors were positive for IGF 1 , 39 for IGF 2 , 48 for IGF IR , and 35 for IGFBP 4 mRNA . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Under IGF 1 supplemented ( 10 nM ) culture conditions , IGF binding proteins ( IGFBPs ) 2 , 4 and 5 were secreted by the growth plate chondrocytes and IGFBP 2 protein and mRNA levels were decreased by the DXM treatment , whereas IGFBP 4 and 5 were not affected . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To test whether two major IGF 1 binding proteins , IGFBP 4 and IGFBP 5 , are related to bone mineral density ( BMD ) , we studied a sample of the Framingham Offspring Cohort participants ( 99 men and 101 women , ages 60 87 ) . ^^^ Serum levels of IGF 1 , IGFBP 4 , and IGFBP 5 were measured by previously validated radioimmunoassays ( CVs approximately 10 % ) . ^^^ In males , but not females , IGF 1 and IGFBP 5 were inversely associated with age ( r = 0 . 34 and r = 0 . 28 , respectively ; P < 0 . 01 ) , while IGFBP 4 levels were positively associated with age ( P < 0 . 01 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Levels of IGFBP 2 , IGFBP 4 , IGF 1 and IGF 2 in media conditioned by 75 day fetal S 5 cultures were not influenced by late DEX . ^^^ Early DEX treatment increased ( P < 0 . 05 ) IGFBP 4 levels in 75 day S 5 media but had little to no influence on levels of IGF 1 , IGF 2 and other IGFBPs . ^^^ These studies indicate that IGFBP 4 may regulate local metabolism during preadipocyte differentiation , whereas IGFBP 3 may antagonize preadipocyte differentiation by targeting IGF 1 away from differentiating cells and towards growing cells . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Human pregnancy associated plasma protein A ( PAPP A ) cleaves insulin like growth factor ( IGF ) binding protein 4 ( IGFBP 4 ) , causing a dramatic reduction in its affinity for IGF 1 and 2 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Eleven weeks of physical training resulted in decreased levels of total IGF 1 , free IGF 1 , and IGFBP 4 in both the UT and WT groups . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To study the roles of insulin like growth factors ( IGFs ) and their binding proteins ( IGFBPs ) in the differentiation of tongue myoblasts , we established a mouse tongue organ culture system and examined the effects of exogenous IGF 1 , exogenous IGFBP 4 , 5 , 6 , and des ( 1 3 ) IGF 1 , an IGF 1 analogue with reduced affinity for IGFBPs , on the differentiation of tongue myoblasts . ^^^ The exogenous IGF 1 stimulated differentiation of tongue myoblasts and induced the expressions of endogenous IGFBP 4 , 5 , and 6 , suggesting that these IGFBPs were involved in the regulation of tongue myoblast differentiation by the IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In late pregnancy , placental weight correlated positively with both maternal plasma IGF 1 and IGFBP 4 , while the surface area of syncytiotrophoblast and weight of trophoblast correlated positively , and mean syncytiotrophoblast thickness negatively , with maternal plasma IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Serum level of insulin like growth factor 1 ( IGF 1 ) rose by 10 % in the milk group ( P < . 001 ) , and the level of insulin like growth factor binding protein 4 ( IGFBP 4 ) fell slightly ( 1 . 9 % ) in the milk group and rose significantly ( 7 . 9 % ) in the control group ( P < . 05 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 bound with high affinity to recombinant IGFBP 3 and IGFBP 4 with K ( D ) s of < 0 . 25 nmol . ^^^ As reported for native IGFBPs , IGF 2 bound with affinity higher than IGF 1 to recombinant IGFBP 3 and IGFBP 4 ( K ( D ) of < 0 . 05 nmol ) . ^^^ Recombinant IGFBP 3 and IGFBP 4 were found to inhibit the IGF induced proliferation of an NIH3T3 cell line engineered to overexpress the IGF 1 receptor . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 levels were also decreased at day 7 in the presence of IGF 1 , potentially by proteolysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Acidosis increased the expression of IGF 1 binding protein 4 ( IGFBP 4 , an inhibitor of IGF 1 activity ) , whereas coincubation with PTH during acidic conditions abrogated the up regulation of IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In normal testes a clear expression of IGF 1 , IGF 2 , IGF IR , IGFBP 2 , IGFBP 4 and IGFBP 5 was found . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
We attribute these effects to the sequestration of IGF 1 by IGFBP 4 and consequent impairment of IGF 1 action in skeletal tissue . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^ When VA deficient rats received a single injection of retinoic acid ( 2 mg / rat ) , tissue IGF 1 and IGF IR gene expression did not change after 4 or 8 h , while the expression of IGF 2 , IGFBP 4 , and IGFBP 6 mRNAs in some tissues increased rapidly . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 , IGFBP 5 , and nonglycosylated IGFBP 3 were shown to significantly enhance binding of IGF 1 to VN , whereas IGFBP 2 and glycosylated IGFBP 3 had a smaller effect . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The four largest follicles ( F 1 F4 ) were dissected and concentrations of steroids , IGFBPs and free IGF 1 and levels of proteolytic activity for IGFBP 4 and 5 in the follicular fluid were determined . ^^^ However , in group 2 the largest follicle ( F 1 ) had higher estradiol , free IGF 1 , and IGFBP 4 and 5 proteolytic activity than F 2 F4 , whereas only slight ( dissected ) or no ( ultrasound ) differences in diameters could be detected . ^^^ Differences between F 1 and F 2 F4 in diameter , estradiol , free IGF 1 , and IGFBP 4 and 5 proteolytic activity were even greater in group 3 . ^^^ The results suggest that follicular selection is a progression of changes starting with acquisition of an FSH inducible IGFBP 4 / 5 proteolytic activity , leading to an increase in intrafollicular concentration of free IGF 1 that , in turn , synergizes with FSH to promote greater estradiol production by the follicle destined for dominance . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 ( 1 nM ) enhanced release of IGFBP 3 while dexamethasone ( 1 nM ) diminished release of IGFBP 4 . ^^^ Furthermore , the specific stimulatory and inhibitory effects of IGF 1 and dexamethasone , respectively , on release of the predominant species of IGFBP 3 and IGFBP 4 , suggested that IGFBP production may be selectively modulated in a positive and negative manner . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 binds IGF 1 and IGF 2 with similar affinities and inhibits their actions under almost all in vitro and in vivo conditions . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
When the effects of individual IGFBPs were tested , IGFBP 2 and IGFBP 4 were found to inhibit IGF 1 stimulated DNA synthesis and migration . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
These results suggest that the insulin deficiency and chronic hyperinsulinemia in diabetes upregulate the IGF 1 receptor and downregulate IGFBP 4 and IGFBP 5 in the aorta . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated [ ( 3 ) H ] thymidine incorporation was significantly enhanced by pretreatment with 1 nm TNFalpha , and this enhancement was blocked in the presence of protease resistant IGFBP 4 . ^^^ In conclusion , PAPP A expression is regulated by inflammatory cytokines in adult human fibroblasts , with functional consequences on IGFBP 4 protease activity and IGF 1 bioavailability . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Soluble IGFBP 5 and IGFBP 4 depressed the binding of ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 to the IGF 1R , but did not affect binding of ( 125 ) 1 R ( 3 ) IGF 1 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 induced IGFBP 4 proteolysis by PAPP A may enhance cell growth and invasion through IGF 1 dependent Akt and ERK1 / 2 activation and subsequently upregulation of uPA . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGFBP 4 is an inhibitor of IGF 1 in bone . ^^^ We show that TGF beta regulates IGFBP 4 and enhances IGF 1 stimulated growth of cultured human bone cells through increased expression of an IGFBP 4 protease , PAPP A . ^^^ IGF 1 stimulation of hOB cell proliferation was markedly enhanced by pretreatment with TGF beta and [ Leu 27 ] IGF 2 , and this enhancement was prevented with protease resistant IGFBP 4 . ^^^ These data support a model whereby local TGF beta and IGF 2 in the bone microenvironment coordinately amplify IGF 1 bioavailability through controlled IGFBP 4 proteolysis , which may be a means to promote bone formation . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
E 2 , P 4 , leptin and IGF 1 levels in serum or follicular fluid ( FF ) were measured with radioimmunoassay ; cyclin D 2 , EGF receptor ( EGF R ) , and inhibin alpha mRNA levels in luteinized granulosa cells were measured with RT PCR ; and IGFBP 4 and PAPP A levels in FF were measured with Western blot analysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , levels and / or sites of expression of IGF 1 , IGF 2 , IGFBP 4 , IGFBP 5 and type 2 receptor in follicular cells strongly differ between mammalian species , suggesting that these phenomena might play species specific or secondary roles in ovarian folliculogenesis . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
T 3 stimulates Sertoli cell lactate secretion as well as mRNA expression of inhibin alpha , androgen receptor , IGF 1 , and IGFBP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Neither tissue microdialysate nor serum concentrations of IGF 1 and IGF binding proteins ( IGFBP 3 and IGFBP 4 ) or procollagen type 1 N terminal propeptide changed from resting values . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 increased IGFBP 4 mRNA levels by 2 fold in both cell types . ^^^ IGFBP 4 protein was only detectable in media conditioned by BME cells when stimulated by IGF 1 . ^^^ In contrast , IGFBP 4 was present in media conditioned by untreated BMFs but was not consistently increased by IGF 1 treatment . ^^^ This was explained by the finding that IGF 1 stimulated proteolysis of IGFBP 4 , as evidenced by the appearance of two immuno responsive fragments of 18 and 14 kDa . ^^^ In vitro immuno neutralization experiments showed that blocking PAPP A prevented the ability of IGF 1 to stimulate IGFBP 4 proteolysis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : ISH revealed a homogeneous distribution of IGFBP 1 , IGFBP 4 and IGF 1 mRNA over hepatic parenchyma in normal and cirrhotic liver . ^^^ Fibrous septa of cirrhotic liver were IGFBP 1 mRNA negative , whereas IGFBP 4 and IGF 1 transcripts were detected in single cells . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Ligand blotting revealed that both IGF 1 and 2 increased IGFBP 2 and 5 ( protein ) and had no effect on IGFBP 3 ( protein ) , whereas IGF 1 ( but not IGF 2 ) increased IGFBP 4 ( protein ) , suggesting IGFBP 2 , 4 , and 5 are post transcriptionally regulated . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Here , we report an 10 ray structure of the binary complex of IGF 1 and the N terminal domain of IGFBP 4 ( NBP 4 , residues 3 82 ) and a model of the ternary complex of IGF 1 , NBP 4 , and the C terminal domain ( CBP 4 , residues 151 232 ) derived from diffraction data with weak definition of the C terminal domain . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : Clear cell RCC were characterized by significant increases in the mRNA expression of IGF 1 , IGFBP 3 and IGFBP 6 while papillary RCC exhibited down regulated expression of IGF 1 , IGFBP 4 and IGFBP 5 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In contrast , bone IGFBP 4 abundance was relatively unchanged by either age or IGF 1 overexpression . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Unlike IGFBP 4 KO mice , however , the triple KO mice became significantly smaller by adulthood ( 78 % wild type ) and had significant reductions in fat pad accumulation ( P < 0 . 05 ) , circulating levels of total IGF 1 ( 45 % of wild type ; P < 0 . 05 ) and IGF 1 bioactivity ( 37 % of wild type ; P < 0 . 05 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Of these , IGFBP 4 and 5 could potentially influence the effect of IGF 1 in the tendon because they both are produced in fibroblast ; however , the response of IGFBP 4 and 5 to mechanical loading and their role in IGF 1 regulation in tendinous tissue are unknown . ^^^ In conclusion , loading of tendon tissue results in an upregulation of IGF 1 , IGFBP 4 , and procollagen and is associated with an increase in tendon mass . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
There was no correlation of plasma IGFBP 4 level with age , sex , immunophenotype , or ALL risk group , and there was no correlation of IGFBP 4 level with plasma IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 levels . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
RESULTS : IGF 1 and IGFBP 4 concentrations were lower whereas the concentration of IGFBP 6 was higher in synovial fluids from patients with prosthesis loosening than in synovial fluid from patients with osteoarthrosis . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the roles of IGFBP 4 and 5 in age dependent bone loss and vertebral fracture risk in postmenopausal Japanese women and to compare them with those of IGF 1 and IGFBP 3 . ^^^ Serum levels of IGFBP 4 and 5 as well as IGF 1 and IGFBP 3 were measured by radioimmunoassay . ^^^ Serum levels of IGF 1 , IGFBP 3 , and IGFBP 5 declined with age , while serum IGFBP 4 increased with age . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Since neither U87MG nor HBEC cells secreted detectable levels of IGF 1 , and there are no known cellular IGFBP 4 receptors , the anti angiogenic effect of IGFBP 4 was likely IGF 1 independent and indirect . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Comparison of follicular fluid IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and PAPP A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF embryo transfer patients . ^^^ This is the first study that has compared follicular fluid ( FF ) IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and pregnancy associated plasma protein ( PAPP ) A concentrations , and their ratios , to investigate whether there was any difference in the intrafollicular microenvironment between the GnRH agonist ( GnRHa ) and antagonist ( GnRHant ) protocols for controlled ovarian stimulation ( COS ) . ^^^ IGF 1 , IGF 2 and IGFBP 3 concentrations were measured by radioimmunoassay and IGFBP 4 and PAPP A by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In addition , plasma GH profiles and plasma concentrations of IGF 1 , IGF 2 , and insulin like growth factor binding protein 4 and 6 ( IGFBP 4 and 6 ) were measured to assess their potential systemic role during bone formation . ^^^ Plasma concentrations of GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 did not demonstrate any significant differences between treatment groups and controls . ^^^ Changes in the systemic osteotropic growth factors GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 do not seem to be of importance during distraction osteogenesis . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
To analyze the effects of N and C terminal domain interactions , we determined several 10 ray structures : first , of a ternary complex of N and C terminal domain fragments of IGFBP 4 and IGF 1 and second , of a `` hybrid ' ' ternary complex using the C terminal domain fragment of IGFBP 1 instead of IGFBP 4 . ^^^ We also solved the binary complex of the N terminal domains of IGFBP 4 and IGF 1 , again to analyze C and N terminal domain interactions by comparison with the ternary complexes . ^^^ Although the C terminal domains do not form stable binary complexes with either IGF 1 or the N terminal domain of IGFBP 4 , in the ternary complex , the C terminal domain contacts both and contributes to blocking of the IGF 1 receptor binding region of IGF1 . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
In animals insulin like growth factor 1 ( IGF 1 ) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 ( IGFBP 4 ) . ^^^ However , the presence of IGF 1 and IGFBP 4 in human tendon tissue is not described . ^^^ Tissue IGF 1 content was examined by immunoflourometric assay , real time PCR , and immunohistochemistry used to localize and determine expression of IGF 1 and IGFBP 4 in 6 postmortem human Achilles tendons . ^^^ Furthermore , we demonstrated that IGF 1 and IGFBP 4 are localized around the tendon fibroblasts and that mRNA for IGF 1 and IGFBP 4 can be determined in human tendon tissue . ^^^ The present study adds support for the roles of IGF 1 and IGFBP 4 in the regulation of tendon adaptive responses to mechanical loading . . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Ribonuclease protection assays done on total RNA from muscle showed that testosterone administration increased mRNA concentrations of IGF 1 and decreased mRNA concentrations of insulin like growth factor binding protein 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated osteocalcin containing Gla secretion was inhibited by one of its binding proteins ( insulin like growth factor binding protein 4 ) in a concentration dependent manner . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Signaling by insulin like growth factors in paralyzed skeletal muscle : rapid induction of IGF 1 expression in muscle fibers and prevention of interstitial cell proliferation by IGF BP 5 and IGF BP 4 . ^^^ To determine whether IGFs may participate in the initiation of restorative reactions in inactivated muscle we analyzed the expression of IGF 1 and IGF 2 mRNA in botulinum toxin paralyzed and in denervated rat skeletal muscle , and interfered with the activity of IGFs by locally applying the IGF binding proteins IGF BP 5 and IGF BP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
The affinity of HT 29 IGFBP for insulin like growth factor ( IGF ) 2 ( approximately 3 . 4 10 10 ( 10 ) M 1 ) is slightly greater than its affinity for IGF 1 ( approximately 1 . 4 10 10 ( 10 ) M 1 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
IGF 1 stimulated mainly IGF BP 3 production , but epidermal growth factor ( EGF ) and transforming growth factor alpha ( TGF alpha ) stimulated predominantly IGF BP 4 secretion . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Undifferentiated P 19 cells synthesized IGF 1 and 2 , the type 1 and 2 IGF receptors , and IGF binding proteins ( IGF BP 2 , IGF BP 3 , and IGF BP 4 ) . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
Serum concentrations of IGF 1 , IGF 2 , BP 1 , and BP 3 were determined by immunoradiometric assays , and Western ligand blots provided a semiquantitative measurement of BP 2 , BP 3 , and BP 4 . ^^^
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA
Interacting proteins: P22692 and P05019	Pubmed	SVM Score :0.0
NA