Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.55559003
Serum IGF 1 was highly correlated with IGFBP 3 before any treatment ( r = 0 . 433 , P < 0 . 001 ) . 0.55559003^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.51503186
IGFBP 3 showed a significant correlation with IGF 1 , IGF 2 , and free and total PGH at K 28 and K 36 . 0.51503186^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.81401364
The interaction between the IGF 1 and its receptor is mainly regulated by a binding protein , IGFBP 3 . 0.81401364^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.62461175
Structural analogs of recombinant human insulin like growth factor 1 ( IGF 1 ) , with alterations to each of the B , C , A , and D domains , have been tested for their ability to form binary complexes with IGF binding protein 3 ( IGFBP 3 ) and ternary complexes with IGFBP 3 and the acid labile subunit ( alpha subunit ) . 0.62461175^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.57502918
Furthermore , they suggest that processing of cell associated IGFBP 3 to forms with altered affinity for IGF 1 peptide may underly the potentiating effect of IGFBP 3 on IGF 1 action . . 0.57502918^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.59353761
Multivariate regression analyses between IGF 1 or IGFBP 3 with age , sex , and estradiol or testosterone show significative correlation in prepubertal children for IGF 1 ( r = 0 . 545 , p = 0 . 0001 and r = 0 . 574 , p = 0 . 0001 for girls and boys , respectively ) and only in prepubertal boys for IGFBP 3 ( r = 0 . 336 , p = 0 . 0012 ) . 0.59353761^^^ The linear correlation between IGF 1 and IGFBP 3 was significant in both prepubertal ( r = 0 . 25 , p < 0 . 0001 ) and pubertal ( r = 0 . 40 , p < 0 . 0001 ) girls , but only in prepubertal boys ( r = 0 . 30 , p < 0 . 0001 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . 0.52674283^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.55418324
We report the relationship between serum IGF 1 , IGF 2 , their binding proteins IGFBP 1 and IGFBP 3 , and GH binding protein in 600 normal Spanish children who were divided into 5 groups according to Tanner stage : 1 , 150 males and 102 females ; 2 , 40 males and 42 females ; 3 , 45 males and 45 females ; 4 , 42 males and 55 females ; and 5 , 23 males and 56 females . 0.55418324^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.54581039
The aim of this work was to investigate interactions between IGF 1 and the PA / PAI system and their influence on IGFBP 3 production and proteolysis in this cell model . 0.54581039^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.86774192
IGF 1 delivered as a complex with glycosylated IGFBP 3 was found to circulate as a 140 kD complex at 4 and 8 h post injection . ( ABSTRACT TRUNCATED AT 250 WORDS ) . 0.86774192^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.53978149
After purification by immunoaffinity chromatography , non pregnancy and pregnancy IGFBP 3 formed ternary complexes with IGF 1 equally well , although the pregnancy proteolysed protein appeared degraded to approximately 30 kDa . 0.53978149^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.86645715
RESULTS : African Pygmies did not differ from Europeans or Bantus in mean serum IGF 1 concentrations , nor in the relationship between serum IGF 1 and its major binding protein ( IGFBP 3 ) . 0.86645715^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.53438352
In contrast , des ( 1 3 ) IGF 1 manifested a remarkably higher potency as compared with IGF 1 in inducing short and middle term cellular responses in IGF IR transfected Ishikawa endometrial cancer cells possessing a high number of both the receptor and the cell membrane bound IGFBP 3 . 0.53438352^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.55926842
As no relationship was found between IGF 1 , IGFBP 3 and Hb levels at the second examination , the same association could not be demonstrated at two months of age . . 0.55926842^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.73989657
Although IGF 1 may enhance remyelination , these results indicate that administration of IGF 1 associated with IGFBP 3 may also accentuate autoimmune demyelinating disease . . 0.73989657^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.6375442
A linear correlation was found between IGF 1 and IGFBP 3 which was positive for controls ( r=0 . 946 , p < 0 . 001 ) and patient group A and B ( r=0 . 839 , p = 0 . 005 ) but tended to be negative for patient group C ( r=0 . 65 , n . s . ) . 0.6375442^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.51089321
Plasma IGFBP 3 showed no association with lung cancer risk unless adjusted for IGF 1 level ; when both of these variables were analyzed together , high plasma levels of IGFBP 3 were associated with reduced risk of lung cancer ( OR = 0 . 48 ; 95 % CI = 0 . 25 0 . 92 ; P = . 03 ) . 0.51089321^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.65771916
OBJECTIVE : To determine the effects of recombinant human insulin like growth factor 1 ( IGF 1 ) complexed with its principal binding protein , IGFBP 3 , on skeletal muscle metabolism in severely burned children . 0.65771916^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.56530543
Relationships between IGF 1 , IGFBP 3 and GH after glucose load were assessed , as well as differences between IGF 1 and IGFBP 3 levels in active and inactive acromegaly . 0.56530543^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.74552602
However , the normal inverse relationship between free IGF 1 and IGFBP 1 was absent , and free IGF 1 changed in parallel with total IGF 1 and thus ALS and IGFBP 3 . 0.74552602^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.82711836
CONCLUSIONS : These results are consistent with the idea that PSA can modulate in vitro interactions between IGF 1 and IGFBP 3 and suggest that PSA may play a role in the regulation of human prostatic fibromuscular cell growth . . 0.82711836^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.53144887
The proportion of IGF 1 associated with IGFBP 3 remained constant throughout life , but was significantly lower in GHD due to an increase in IGF I / IGFBP 2 complexes . 0.53144887^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.55853763
IGF 1 circulates as a ternary complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . 0.55853763^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.64097342
A linear correlation was found between IGF 1 and IGFBP 3 which was positive for controls ( r = 0 . 946 , p < 0 . 001 ) and patient group A and B ( r = 0 . 839 , p = 0 . 005 ) but tended to be negative for patient group C ( r = 0 . 65 , n . s . ) . 0.64097342^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.67798769
Thus , IGFBP 3 might be initially expressed as a cell associated form and then released in the interstitial fluid after a direct interaction with IGF 1 . 0.67798769^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.55077873
IGF 1 circulates predominantly as a ternary complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . 0.55077873^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.90701271
Most serum IGF 1 associates with IGF binding protein 3 ( IGFBP 3 ) and another protein , the acid labile subunit ( ALS ) . 0.90701271^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :1.0323191
The administration of recombinant human IGF 1 complexed with its predominant binding protein IGF binding protein 3 ( rhIGF I / IGFBP 3 ) may allow the safe administration of higher doses of IGF 1 than can be accomplished with rhIGF 1 alone . 1.0323191^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.60739059
AIMS : To examine the relationship between IGF 1 and its major plasma binding protein , IGF binding protein 3 ( IGFBP 3 ) , and the risk of colorectal cancer . 0.60739059^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.53722386
The association between IGF 1 or IGFBP 3 and prostate cancer risk was assessed using conditional logistic regression , and paired t tests were used to evaluate case control differences in change in serum analytes over time . 0.53722386^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.69210207
We examined the association between IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 3 protease activity and breast cancer risk in postmenopausal women , taking ER status of the breast cancer into consideration . 0.69210207^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :1.0591576
However , Val44Met IGF 1 binds IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and IGFBP 6 with equal affinity to IGF 1 , suggesting the maintenance of overall structure , particularly in the IGFBP binding domain . 1.0591576^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.59890915
The majority of circulating IGF 1 is bound in a complex with IGF binding protein 3 ( IGFBP 3 ) , which in turn limits IGF 1 bioavailability . 0.59890915^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.61054578
In addition , the serum half life of unbound IGF 1 is shorter when administered to patients with GHIS , who have low serum concentrations of its primary binding protein IGFBP 3 than when administered to healthy individuals or to patients with an IGF 1 gene deletion ( who have normal levels of IGFBP 3 ) . 0.61054578^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :1.1419621
Some prospective studies support a direct association between IGF 1 and prostate cancer , in particular advanced disease , whereas both inverse and direct associations with prostate cancer have been reported for insulin like growth factor binding protein 3 ( IGFBP 3 ) , the major IGF 1 binding protein in circulation . 1.1419621^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.93387549
The association between IGF 1 and breast cancer was determined using conditional logistic regression , adjusting for IGFBP 3 . 0.93387549^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.58798239
Hypoglycaemia has been reported with administration of unbound IGF 1 and , in addition , the serum half life of unbound IGF 1 is shorter when administered to patients with GHIS ( who have low serum concentrations of its binding protein IGFBP 3 ) than when administered to normal volunteers or to patients with an IGF 1 gene deletion ( but normal IGFBP 3 levels ) . 0.58798239^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.64443
We tested the association between IGF 1 and IGFBP 3 and prostate cancer risk by measuring the two peptides in plasma samples collected at baseline in a prospective cohort study of 17 , 049 men . 0.64443^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF binding protein 3 is related to IGF 1 , but not to spontaneous GH release , in healthy old men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 concentrations were significantly correlated ( Spearman ' s r = 0 . 43 , p less than 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Cleavage of IGFBP 3 by PSA resulted in a marked reduction in the binding affinity of the fragments to IGF 1 , but not IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both , reduced IGF 1 production and increased binding of IGF to IGFBP 3 result in decreased IGF bioactivity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Administration of IGF 1 slightly increased IGFBP 3 and a smaller 32 kDa IGFBP at days 17 and 20 of pregnancy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously shown that IGF 1 administration increases IGFBP 1 and 2 and reduces IGFBP 3 in normal subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , the simultaneous presence of both EGF and IGF 1 reduces the level of IGFBP 3 more efficiently than EGF alone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The intensity of the IGFBP 3 bands changed in parallel with serum IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The hepatic expressions of mRNA encoding IGF 1 and IGFBP 3 were reduced and IGFBP 1 mRNA was elevated in the SDR . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Interestingly , only 4 IU resulted in a significantly greater increase in IGF 1 than in IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that production of predominantly IGFBP 3 by the HaCaT cell line modulates its sensitivity to IGF 1 stimulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Like GH , IGF 1 normalized IGFBP 3 mRNA levels in liver , but , in contrast to GH , had no effect on IGFBP 5 mRNA in WAT . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Proteolysis of IGFBP 3 results in an increase in IGF 1 clearance and possibly in IGF bioavailability . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 was stimulated by IGF 1 , IGFBP 4 by forskolin , and IGFBP 5 by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both prednisolone and GH alleviated the hypoglycemia , concomitantly with increases in IGF 1 , IGFBP 3 , and ALS levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neutralizing antibodies directed against either IGF 1 or IGF 2 or IGF binding protein 3 blocked protein accumulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Primary antisera were directed against human IGF 1 , human IGF BP 1 , bovine IGF BP 2 , and human IGF BP 3 respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , IGF 1 and 2 can attenuate inhibitory effect of IGFBP 3 by forming IGF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Nocturnal levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 remained stable during both studies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma immunoreactive IGF 1 and IGF 2 , and serum IGFBP 1 levels were normal , but serum levels of IGFBP 3 were increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , IGF 1 does not appear to mediate the GH effect on IGFBP 3 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Most of the circulating IGF 1 is bound to three IGF binding proteins ( BP ) , mostly IGFBP 3 , BP 2 and BP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Enhanced cell responsiveness to IGF 1 appears to be due to IGF 2 induced changes in pericellular IGFBP 3 and IGFBP 4 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF binding protein 3 levels paralleled the rise in IGF 1 consistent with a common regulatory mechanism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In cocultures , however , synthesis of IGFBP 3 was stimulated by insulin and IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In summary , TBA+E2 increased serum concentrations of both IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 were measured by radioimmunoassay while all IGFBP were evaluated by Western ligand blotting . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and / or GH increased serum concentrations of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 rose , IGF 2 decreased , and IGFBP 3 remained constant . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , the ternary complex and its components , IGF 1 , IGFBP 3 and ALS increased with gestation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , short term refeeding induced an increase in the serum level of IGFBP 3 , the major binding protein for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH measurement in serum may be accompanied by IGF 1 and IGFBP 3 measurements , and in some patients by GHBP measurement . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 was able to bind to all IGF 1 forms . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Fasting measurements of glucose , insulin , IGF 1 , IGF 2 , IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both treatments increase serum ALS , IGFBP 3 , and IGF 1 levels , and decrease IGFBP 2 , whereas IGFBP 6 is unaffected . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The 22 to 25 kD fragment had severely reduced affinity for IGF 1 , compared with intact IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Values of IGF 1 , IGF 2 , and IGF BP 3 were low . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Parallel to the increase in GH secretion at puberty , circulating IGF 1 and IGF binding protein 3 also increase . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGFBP 3 was increased by 4 h of IGF 1 treatment in fasted but not in fed lambs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 5 but not IGFBP 3 stimulated thymidine incorporation into DNA both in the absence and presence of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Testosterone levels were measured hourly and insulin , SHBG , IGF 1 , and IGFBP 3 levels at 0800 h . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of HbA1c , IGF 1 , and IGFBP 3 were determined at 0700 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total IGF 1 exhibited minor changes only during the clamp ( P < 0 . 05 ) , and IGFBP 3 was unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGFBP 1 , and IGFBP 2 levels were measured by radioimmunoassay , and IGFBP 3 by immunoradiometric assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 stimulated mainly IGFBP 3 production , while EGF stimulated predominantly IGFBP 4 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In jejunal mucosa , IGF 1 and GH treatment independently and synergistically stimulated IGFBP 3 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 and IGF BP 3 were below normal levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 and IGFBP 3 concentrations were determined by means of radioimmunoassay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Human IGFBP 3 and rIGFBP 3 were able to compete for tracer binding in the presence , but not in the absence , of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGFBP 3 and GHBP were all decreased or at the lower limit of the normal range . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentration of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 increased during treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum immunoreactive IGFBP 3 levels fell and IGFBP 2 and 3 levels rose during IGF 1 therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , blood samples for hormonal analysis , IGF 1 , IGFBP 3 , GHBP , and leptin were drawn . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were measured in blood samples collected in 1989 90 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGFBP 3 but not IGF 1 were significantly lower than those in normal controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 markedly increased IGFBP 3 mRNA levels in jejunum after both transection and resection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Comment on dried blood spot assay of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neither IGF 1 nor IGF binding protein 3 concentrations changed significantly during the short period of GH stimulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concomitant administration of IGF binding protein 3 blocked IGF 1 positive inotropic action in ferret papillary muscles . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Gene expression of IGF 1 and IGFBP 3 was demonstrated by reverse transcription polymerase chain reaction in BAL cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 and IGF BP 3 were below normal levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Phosphorylation of human IGFBP 3 , however , does not affect its affinity for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , peritoneal clearance of IGF 1 and 2 was similar to that of the larger and more abundant IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased levels of IGF 1 and IGFBP 3 in synovial fluids of patients with rheumatoid arthritis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurements of ALS and IGFBP 3 added no useful extra information to IGF 1 in managing the dose titration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , Nx did not alter the levels of IGF 1 , IGFBP 2 , and IGFBP 3 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
He had elevated GH levels , normal levels of IGFBP 3 , undetectable levels of IGFI , and showed no response to GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No correlation was observed between leptin and IGF 1 or IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
C 6 cells produce IGFBP 3 , IGFBP 4 , a negligible amount of IGFBP 2 , and IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Influence of peritoneal loss of GHBP , IGF 1 and IGFBP 3 on serum levels in children with ESRD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 and IGFBP 3 levels are low in children with chronic liver failure despite increased GH secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The low concentrations of serum IGF 1 and IGFBP 3 became normal during GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Liver RNA was analyzed for mRNAs specific for the GH receptor , IGF 1 , IGF 2 , and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared to normal subjects , free IGF 1 and IGFBP 3 were significantly elevated , and IGFBP 1 was decreased upon AMI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Finally , we showed that atRA induced IGFBP 3 is functionally active in modulating the growth promoting effect of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 levels were also studied . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 increased more than IGFBP 3 whereby its biological activity obviously improved . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 , and acid labile subunit rose in response to GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 was the predominant binding protein reflecting IGF 1 concentrations and body size . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The assessment of IGFBP 3 is the most spread among all IGFBPs in the studies of GH IGF 1 axis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone ( GH ) therapy raises both IGF 1 and IGFBP 3 levels in serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 levels were elevated with infusion of IGF I / BP 3 compared with IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline serum IGF 1 and IGFBP 3 were low , while IGFBP 1 and IGFBP 2 were raised . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Exposure to IGF 1 or IGF 2 ( 13 or 26 nM ) caused an increase in expression and release of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The liver is the major source of IGF 1 and at least two IGFBPs ( IGFBP 1 and IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum ALS levels were significantly correlated with serum IGF 1 levels and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
SF analysis showed that IGF 1 and IGFBP 3 levels were increased in post exercise SF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The metabolic activity of IGF 1 is regulated by 6 IGF binding proteins . the most important being IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 and prostate cancer risk : epidemiological studies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 1 and IGFBP 3 were low , while insulin , C peptide and GH were undetectable . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These include reductions in growth hormone , IGF 1 , IGFBP 3 , and IGFBP 5 and an increase in IGFBP 4 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examine the effect of insulin like binding protein 3 ( IGFBP 3 ) both in the presence and absence of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
EGF decreased IGF binding protein 2 ( IGFBP 2 ) and IGFBP 3 by more than 50 % in the presence of IGF 1 on PL or collagen 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The intensity of IGF 1 binding to the 30 kDa IGFBP was much higher than that of IGFBP 3 , found in control rat serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The highest diagnostic efficiencies for IGF 1 and IGFBP 3 were observed during placebo ( 75 % and 91 % , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH BP , IGF 1 and IGFBP 3 serum levels were not changed significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF binding protein 3 behavior paralleled that of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increase of IGF 1 mRNA and IGF binding protein 3 ( IGFBP 3 ) accumulation occur without any effect on prostate weight . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No influence of T ( 3 ) on IGF 1 , IGF 2 , IGFBP 3 , or IGFBP 4 mRNA levels in hOB was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 and IGF binding protein 3 increased with GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of raloxifene on IGF 1 and IGFBP 3 in postmenopausal women with breast cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 1 , and IGFBP 3 showed lower adjusted means for both Hispanics and Asians compared with Caucasians . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 treatment increased the serum levels of IGF 1 and IGFBP 3 in both control and LID mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition of IGFBP 3 to aliquots of SFM CM reversed the IGF 1 mediated acceleration of IGFBP 4 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similarly , TNFalpha values did not correlate with either IGF 1 or IGFBP 3 serum concentrations . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting lipid profile , IGF 1 , IGFBP 3 , overnight GH profiles and free insulin concentrations were also assessed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant differences were found in trabecular bone content of IGF 1 , IGFBP 3 , or osteocalcin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total IGF 1 , IGFBP 3 and ALS possess a higher PV ( neg ) for the clinical disease activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Inappropriate serum levels of IGF 1 and IGFBP 3 in patients with rheumatoid arthritis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ( 30 % ) , IGF binding protein 3 ( 18 % ) , testosterone ( 50 % ) , and SHBG ( 40 % ) were reduced in TS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 were normal . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These actions of IGFBP 3 in the MCF 10A cells were independent of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Re : Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 as predictors of advanced stage prostate cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting serum samples were analyzed for total and free IGF 1 and 2 , for IGFBP 1 to 4 , as well as for IGFBP 3 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For younger subjects , IGF 1 was inversely associated with lung cancer risk in the models that also controlled for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurements of IGF 1 , IGF 2 and IGF binding protein 3 concentrations were performed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased duration of anovulation was associated with decreased concentrations of IGF 1 and IGFBP 3 in FFL . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 but not IGFBP 3 levels decreased in all groups ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH increased IGF binding protein 3 ( IGFBP 3 ) , IGF 1 receptor protein and its phosphorylation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 , PICP , ICTP ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 levels did not decline during DEXA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Captopril had no effect on IGF 1 mRNA but further increased IGFBP 3 mRNA and protein in the border zone , ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 and IGFBP 3 were not related to any of the physical characteristics . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The increment was significantly greater in group 2 as was the increment in serum IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Diagnostic value of IGF 1 and IGFBP 3 for growth hormone deficiency in children ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 6 were examined by specific assays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The r hGH treatment groups had similar serum IGF 1 and IGFBP 3 responses , both significantly different from placebo . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Risk was unrelated to levels of IGF 1 , IGFBP 2 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
FFM was positively and significantly correlated with concentrations of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP protease activity was present in thyroid tissue and resulted in a decrease in IGFBP 3 affinity for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 normalised in 1 ( 12 . 5 % ) and 3 ( 38 % ) patients respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The decline of free IGF 1 correlated with decreasing levels of IGFBP 3 and increasing levels of IGFBP 1 / 4 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Normal hormone concentrations included IGF 1 , IGF BP 3 , insulin , parathyroid hormone , leptin , and testosterone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant changes were observed in serum levels of testosterone , IGF 1 , IGFBP 3 , or 5 OHmdU . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 , and cancer risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF binding protein 3 and breast cancer risk : comparison of 3 meta analyses . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The plasma IGF 1 and IGFBP 3 levels were drastically reduced . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Endometrial mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 increased in bST C , but not in bST P cows . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF binding protein 3 , and lipid concentrations were also measured centrally . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH exposure , IGF 1 and IGFBP 3 concentrations ) was assessed using regression techniques . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GHRA treatment equally reduced circulating IGF 1 and IGFBP 3 levels , while apoptosis was increased in the treatment group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lactoferrin and vitamin A did not interact with respect to the IGF 1 and IGFBP 3 status . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURE : Measures of IGF 1 , IGF binding protein 3 , and the molar ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma samples of IGF 1 , IGF 2 , IGF binding protein 3 , and C peptide were collected before initiation of chemotherapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Circulating IGF 1 , IGFBP 3 and IGF I / IGFBP 3 molar ratio were significantly reduced by CEE . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of Hcy , IGF 1 and IGFBP 3 were measured at baseline , and at 1 month and 3 months after treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effect of IGFBP 3 was independent of the blockade of the IGF 1 receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No gender differences were observed with the IGF 1 and IGFBP 3 response . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The mean peak GH was 1 . 25 + / 0 . 75 ng / ml , IGF 1 and IGFBP 3 below 2 SDS with no response to GHRH in those tested . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting blood samples were obtained for measurement of glucose , insulin , IGF 1 , IGFBP 1 , IGFBP 3 and lipid levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This treatment significantly increased GH , IGF 1 , and IGFBP 3 , whereas serum cortisol decreased ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Multiple regression analysis showed no significant effect of age , body mass index and years since menopause on IGFBP 3 levels ; however , considering the IGF I / IGFBP 3 ratio as a possible parameter of circulating free somatomedin C , an inverse correlation was found with years since menopause ( n = 43 , r = 0 . 499 , p < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The alteration seen in IGF 1 distribution after surgery did not appear to be a direct result of the IGFBP 3 proteolytic activity or an effect of the addition of heparin to the circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
FSH , which stimulates cAMP in the Sertoli cell , markedly lowers the concentration of insulin like growth factor binding protein 3 ( IGFBP 3 ) in Sertoli cell conditioned medium ; in contrast , insulin like growth factor 1 ( IGF 1 ) increases BP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PGE 2 rapidly increases IGF 1 and IGFBP 3 mRNA expression in calvaria cells , with a time course clearly different from that observed in response to growth hormone . ^^^ Thus , PGE 2 modifies not only the synthesis of IGF 1 but also that of IGFBP 3 in skeletal tissue . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) and insulin like growth factor 1 ( IGF 1 ) levels were measured by specific radioimmunoassays in children with all degrees of chronic renal failure ( CRF ) . ^^^ Study group 1 comprised 29 children ( 10 on dialysis ) who had been studied one to four times over 2 years to determine whether IGF 1 and IGFBP 3 levels differed from those in age matched healthy children and to examine the relationship between these levels and heights . ^^^ IGF 1 and IGFBP 3 levels did not differ from those in normal children . ^^^ IGF 1 and IGFBP 3 were significantly correlated , increased with pubertal stage in all children and with age in non dialysis patients . ^^^ IGF 1 , but not IGFBP 3 , correlated with age in dialysis patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before treatment , most patients had increased basal serum concentrations of growth hormone ( 2 . 4 208 mU / l ) and low serum concentrations of IGF 1 ( < 20 69 micrograms / l ) , IGF 2 ( 69 295 micrograms / l ) and IGF binding protein 3 ( 0 . 16 1 . 59 mg / l ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , we sought to determine by Western ligand blotting the effects of growth hormone , IGF 1 and IGF 2 on the production of IGFBP 3 and IGFBP 4 in TE 89 human osteosarcoma cells and in untransformed normal human bone cells derived from rib . ^^^ IGF 1 and IGF 2 , which increased human bone cell proliferation , decreased the level of IGFBP 4 ( 30 % of control at 100 micrograms / l IGF 1 and IGF 2 ) but increased the level of IGFBP 3 ( 3 10 fold at 100 micrograms / l IGF 1 and IGF 2 ) after 48 h of treatment in the conditioned medium of both low and high density TE 89 cell cultures . ^^^ Similar changes in IGFBP 3 and IGFBP 4 levels were also seen in the conditioned medium of human bone cells derived from rib after treatment with IGF 1 and IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGF binding proteins ( IGFBP 1 , IGFBP 3 ) , GH and insulin were measured by radioimmunoassay . ^^^ Mean levels of IGFBP 1 and IGFBP 3 , IGF 2 and GH were unaffected by IGF 1 administration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a third group of 40 patients we measured insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGF BP 3 ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both IGFBP 1 and IGFBP 3 produced a dose related inhibition of IGF 1 stimulated oestradiol accumulation in granulosa cell conditioned medium with complete reversal of the effects of IGF 1 in the presence of a molar excess of binding protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Paradoxically , the liver does not appear to be stimulated over control levels by high serum growth hormone levels , since neither IGF 1 peptide , IGF 1 mRNA , nor IGF BP 3 levels are increased in livers of tumor bearing rats . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Administration of IGF 1 , but not GH , resulted in increased levels of IGFBP 3 , the predominant IGFBP of the adult rat . ^^^ While normalization of serum IGF 1 levels does result in modest skeletal growth , selective organ growth and increased serum IGFBP 3 , growth stimulation does not equal that seen with GH ( body weight ) or thyroid hormone ( skeletal growth ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study demonstrates the expression of the rat IGFBP 3 gene in a large number of tissues and coexpression , but not necessarily equal expression , with IGF 1 mRNA . ^^^ These data suggest that IGFBP 3 mRNA abundance is regulated differently in different tissues , and in at least some tissues is less sensitive to regulation than is IGF 1 mRNA . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Binding of [ 125I ] IGF 1 to IGFBP 3 , IGFBP 2 , and IGFBP 1 , measured by ligand blotting , was not statistically different in the 2 groups , but binding intensities for the serum 24 / 25 kDa IGFBP were approximately 2 . 5 times greater in fracture women than control women ( P less than 0 . 0005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These data suggests that the synergistic effects of 1 , 25 ( OH ) 2D3 on the action of IGF 1 on osteoblastic cells , which we reported previously , may be modulated by locally produced IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recent studies have provided a consensus that insulin like growth factor 1 ( IGF 1 ) stimulates IGF binding protein 3 ( IGFBP 3 ) in vivo and in vitro . ^^^ As determined by ligand blot of CM from confluent cells 72 h after the addition of peptides to serum free medium , IGF 1 and IGF 2 potently stimulated IGFBP 3 in both cell lines , but otherwise IGFBP regulation in the two cells diverged . ^^^ In N 3652 cells , IGFBP 3 concentrations in CM increased to 700 % and 800 % of basal levels in the presence of IGF 1 and IGF 2 ( at 100 ng / ml ; n = 5 experiments ) , respectively . ^^^ In SCL 1 cells , IGF 1 and IGF 2 were virtually identical in stimulating a mean 200 % increase in IGFBP 3 ( n = 5 experiments ) . ^^^ To test the hypothesis that IGF induced alterations in IGFBP 3 and IGFBP 4 concentrations were regulated via the type 1 IGF receptor , we attempted to block IGFBP changes with type 1 IGF receptor antibody alpha IR 3 and to induce IGFBP changes with an IGF 2 analog , [ Leu 27 ] IGF 2 , with little affinity for the type 1 receptor . alpha IR 3 failed to block either the IGF induced rise in IGFBP 3 in each cell line or the decline in IGFBP 4 in N 3652 CM . [ Leu 27 ] IGF 2 was as potent as IGF 2 or IGF 1 in inducing changes in IGFBP 3 and IGFBP 4 concentrations . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The increased potencies of the IGF 1 variants may relate to the substantially increased plasma levels of IGF binding proteins , particularly IGFBP 3 , produced by the combined treatment of dexamethasone with IGF 1 or the variants . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Divergent ileal IGF 1 and IGFBP 3 gene expression after small bowel resection : a novel mechanism to amplify IGF action . ^^^ Changes in the levels of ileal insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) mRNA in the rat following massive small bowel resection ( MSBR ) have been investigated with a sensitive S 1 nuclease assay . ^^^ IGF 1 mRNA levels vary little over 7 days ; in contrast IGFBP 3 mRNA levels decreased to one third 7 h post MSBR , and remained suppressed for the length of this study . ^^^ We postulate that decreased ileal synthesis of IGFBP 3 enhances the ability of IGF 1 to stimulate the adaptive response . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine whether chronic hypoxemia results in alterations in endocrine function that may contribute to growth failure , we measured growth hormone ( GH ) , somatomedins ( insulin like growth factors 1 and 2 , IGF 1 and IGF 2 ) , hepatic growth hormone receptors , and circulating IGF binding proteins IGFBP 3 and IGFBP 2 in 12 newborn lambs with surgically created pulmonic stenosis and atrial septal defect , and in 10 controls . ^^^ The increase in IGF 1 was associated with a decrease in IGFBP 3 ( hypoxemic , 5 . 09 + / 1 . 25 vs . control , 11 . 2 + / 1 . 08 arbitrary absorbency units per mm ( Au . mm ) , P less than 0 . 01 ) , and increase in IGFBP 2 ( 0 . 47 + / 0 . 03 vs . 0 . 19 + / 0 . 13 Au . mm , P less than 0 . 05 ) , but no significant downregulation of hepatic GH receptors ( hypoxemic , 106 . 1 + / 20 . 1 vs . control , 147 . 3 + / 25 . 9 fmol / mg , NS ) . ^^^ Thus , chronic hypoxemia in the newborn is associated with a decrease in IGF 1 and IGFBP 3 in the face of normal GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of the study was to investigate the serum levels of IGFBP 3 , the major IGF 1 binding protein , in patients with Laron type dwarfism ( LTD ) before and after IGF 1 treatment . ^^^ Serum IGFBP 3 was abnormally low ( 0 . 70 + / 0 . 37 mg / l ) and decreased significantly further during IGF 1 treatment ( to 0 . 48 + / 0 . 28 mg / l ) ( P less than 0 . 065 ) . ^^^ On the other hand the decrease during IGF 1 administration and concomitant suppression of GH secretion may denote either that GH activity is not completely blocked in this syndrome or that there are additional mechanisms regulating IGFBP 3 synthesis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF infusion ( IGF 1 greater than des IGF 1 ) increased the levels of circulating IGFBP 3 and IGFBP 2 , which may act to modulate the biological effectiveness of the infused peptides . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study we investigated the mechanism ( s ) by which insulin like growth factor binding protein 3 ( IGFBP 3 ) potentiates IGF 1 action in cultured bovine fibroblasts . ^^^ Preincubation of cells with glycosylated or nonglycosylated recombinant human IGFBP 3 enhanced responsiveness to IGF 1 in a time dependent manner . ^^^ Pretreatment with IGFBP 3 for 72 h resulted in a 2 to 4 fold augmentation of IGF 1 stimulated [ 3H ] aminoisobutyric acid uptake ; IGFBP 3 had no effect on basal [ 3H ] aminoisobutyric acid uptake . ^^^ During the preincubation period , exogenous IGFBP 3 associated with the fibroblast surface and exhibited time dependent processing to lower mol wt forms that retained the ability to bind radiolabeled IGF 1 . ^^^ After 72 h of exposure to bovine fibroblasts , cell associated IGFBP 3 had a 10 fold lower affinity for IGF 1 compared to intact IGFBP 3 in solution . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of MECs with either IGF 1 or IGF 2 increased the amount of both the 29K IGFBP and IGFBP 3 in the CM , with relative potencies similar to those of the lactogenic hormones . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of hOB and HOBIT cells with IGF 1 resulted in a 2 fold increase in medium levels of IGFBP 3 ; IGF 1 decreased levels of 24 kilodalton ( kDa ) IGFBP in hOB cell conditioned medium . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin , but not IGF 2 , mimicked IGF 1 ' s action on IGFBP 3 and 2 production , but was less potent . ^^^ The synthetic IGF , long R 3 IGF 1 , which has very low affinity for IGFBPs and only slightly reduced affinity for the IGF 1 ( type 1 ) receptor , had significantly greater potency in stimulating IGFBP 3 and 2 production compared to IGF 1 . ^^^ By Northern analysis the expression of IGFBP 3 mRNA was found to be significantly stimulated by IGF 1 . ^^^ In summary , insulin stimulated IGFBP 3 and 2 production in a manner that mimics that of IGF 1 and the more potent long R 3 IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study we examined the expression of messenger RNA ( mRNA ) encoding IGFBP 3 in cultured bovine and human fibroblasts , two cell lines that secrete IGFBP 3 under IGF 1 control . ^^^ Northern analysis of bovine fibroblast RNA hybridized with a specific bovine IGFBP 3 complementary DNA probe indicated a single 2 . 8 kilobase ( kb ) transcript readily detectable within 2 h in IGF 1 or insulin treated , but not in untreated , cells . ^^^ By Western ligand blotting , IGFBP 3 was not detected in medium from bovine fibroblasts incubated with IGF 1 for 2 , 4 , or 6h , but was apparent after 24 h IGF 1 treatment . ^^^ Furthermore , IGFBP 3 mRNA and protein was induced by different IGF 1 analogs in direct relation to the ability of the peptides to bind to the type 1 IGF receptor , indicating a receptor mediated process . ^^^ In contrast to its effect in bovine fibroblasts , IGF 1 had no significant effect on steady state levels of IGFBP 3 mRNA in cultured human fibroblasts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The affinity of each of the IGFs for individual BPs and the regions of the IGF 1 molecule that are required for this high affinity binding have been defined only for IGFBP 1 and IGFBP 3 . ^^^ In contrast , mutations in the A chain region have minimal effect on binding to IGFBP 3 , whereas substitutions at the 3 , 4 , 15 , 16 positions of the B chain reduce IGF 1 affinity by at least 50 fold . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We speculate that proteolysis of IGFBP 3 may alter tissue uptake of IGF 1 and thereby help to counteract the catabolic state caused by surgery . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
By ligand blotting , both IGF 1 and GH caused a modest increase in IGFBP 3 band intensity . ^^^ The change in IGFBP 3 , while significant , is quantitatively less than that in experimental animals that have been given IGF 1 while undergoing dietary restriction . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Expression of the cDNA clones in mammalian tissue culture cells results in the secretion into the culture medium of ALS activity that can form the expected complex with IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The binding proteins IGFBP 1 and IGFBP 3 did not cross react , but both IGF 1 and IGF 2 markedly inhibited IGFBP 6 tracer binding to antiserum . ^^^ This inhibition , greater for IGF 2 than for IGF 1 , was fully reversed by the addition of IGFBP 3 to sequester the IGFs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When analyzed by SDS PAGE and [ 125I ] IGF 2 ligand blotting , human neonatal fibroblasts secrete IGFBP 3 , an IGFBP of 29 31 kDa , and a 22 24 kDa IGFBP after treatment with 50 ng / ml IGF 1 . ^^^ When IGF 1 treatment was carried out in the presence of increasing concentrations ( 50 1000 ng / ml ) of pure human serum derived IGFBP 3 , there was a dose dependent decrease in the 29 31 kDa protein . ^^^ In the presence of excess ( 250 ng / ml ) IGF 1 , IGFBP 3 had approximately 20 fold reduced potency in inhibiting 29 31 kDa IGFBP . ^^^ When endogenous production of IGFBP 3 was increased by treatment with transforming growth factor beta 1 ( TGF beta 1 ) , there was complete inhibition of 29 31 kDa IGFBP , while at high IGF 1 concentrations TGF beta 1 had 2 to 3 fold reduced potency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the relationship of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , the IGF binding proteins IGFBP 1 , IGFBP 2 , and IGFBP 3 , and GH binding protein ( GHBP ; which is postulated to be derived from the extracellular portion of the GH receptor ) in normal volunteers and patients with anorexia nervosa before and after a refeeding program . ^^^ Serum GHBP , IGF 1 , and IGFBP 3 were all significantly decreased in low weight patients with anorexia nervosa and returned to nearly normal levels with refeeding . ^^^ Both serum IGF 1 and IGF 2 were positively correlated with serum IGFBP 3 and negatively correlated with serum IGFBP 1 and IGFBP 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
On gel filtration chromatography , the majority of circulating IGF 1 in non pregnant and pregnant women was present in the large molecular mass ( 150 kDa ) binding proteins ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein ( IGFBP 3 ) , an inhibitor of serum growth factors other than IGF 1 and 2 . ^^^ Our data show that inhibition by IGFBP 3 of serum stimulation was not simply the result of its inhibition of IGF present in the serum : 1 ) While anti IGF 1 IgG was able to completely inhibit stimulation induced by added IGF 1 , it did not decrease stimulation induced by 1 % human serum . ^^^ The percent inhibition was not significantly increased when the concentration of serum was decreased to 0 . 2 % , which induced 140 % stimulation of DNA synthesis ; 2 ) stimulation by 0 . 2 % serum was much more inhibited by IGFBP 3 than by IgG anti IGF 2 ; 3 ) after separation of IGF 1 and IGF 2 from serum by chromatography of acidified serum proteins on BioGel P 150 , the remaining serum proteins ( with a molecular mass greater than 45 kDa ) which were depleted in IGF 1 and 2 ( verified by RIA determination ) still stimulated DNA synthesis , and this stimulation was 80 % inhibited by IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and transforming growth factor beta 1 release IGF binding protein 3 from human fibroblasts by different mechanisms . ^^^ To examine whether an increase in extracellular concentrations of IGFBPs in response to IGF 1 is due to the release of cell associated IGFBPs , we measured secreted and cell associated IGFBP 3 immunologically in fibroblast monolayers treated with IGF 1 and IGF analogs with altered affinities for the IGF receptors and IGFBPs . ^^^ IGFBP 3 in medium conditioned by fibroblasts treated with IGF 1 was significantly increased ( P < 0 . 05 ) compared with that in medium from untreated cultures ; concomitantly , cell associated IGFBP 3 was significantly decreased ( P < 0 . 05 ) . [ Ser 24 ] IGF 1 ( reduced affinity for IGF receptors ) also increased secreted IGFBP 3 and decreased cell associated IGFBP 3 . ^^^ In contrast , IGFBP 3 concentrations in medium conditioned by fibroblasts treated with B chain IGF 1 ( reduced affinity for IGFBPs ) were not significantly increased , and cell associated IGFBP 3 was unchanged . ^^^ An IGFBP of 29 31 kilodaltons ( kDa ) showed a pattern of regulation similar to that of IGFBP 3 , while a third IGFBP , of 24 kDa , was decreased in IGF 1 and [ Ser 24 ] IGF 1 conditioned medium and unchanged by B chain IGF 1 and heparin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The combination of RA and IGF 1 , which resulted in decreased cellular proliferation , was associated with the appearance of IGFBP 3 in the CM at levels far exceeding those seen with RA alone . ^^^ The effect of IGF 1 on IGFBP 2 levels and the synergistic action of IGF 1 and RA on IGFBP 3 levels in CM were blocked by alpha IR 3 , a monoclonal antibody to the human IGF 1 receptor , indicating that these effects required signal transduction through the IGF 1 receptor . ^^^ The increase in IGFBP 3 protein in CM in response to the combination of RA and IGF 1 was much greater than the increase in IGFBP 3 mRNA . ^^^ These results suggest that the action of RA and IGF 1 in combination to increase IGFBP 3 protein in CM is principally translational or posttranslational . ^^^ We speculate that RA inhibition of IGF 1 stimulated MCF 7 cell proliferation may be due to IGFBP 3 , or that increased levels of IGFBP 3 in response to growth inhibition represent a compensatory response . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In animals given both bromocriptine and anti rGH , concurrent treatment with recombinant bovine GH maintained milk yield at 50 % of control values and normalized serum IGF 1 , IGF 2 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The insulin like growth factors , IGF 1 and IGF 2 , circulate in both humans and rats as part of a 125 150 kDa complex comprising IGFs , the IGF binding protein IGFBP 3 , and an acid labile subunit . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGFs ) circulate in human adult serum predominantly as a high mol wt complex of 150 kilodaltons ( kDa ) , which is made up of three subunits : alpha , the acid labile subunit , a glycoprotein of around 85 100 kDa ; beta , the acid stable IGF binding protein subunit , which is the 40 kDa GH dependent glycoprotein IGF binding protein 3 ( IGFBP 3 ) ; and gamma , the 7 . 5 IGF 1 or 2 peptide subunit . ^^^ When the IGFBPs from the high mol wt complex were cross linked to [ 125I ] IGF 1 or 2 , bands of 48 , 34 , 26 , and 21 kDa , which were more intense with [ 125I ] IGF 2 , were observed , and all were immunoprecipitable by anti IGFBP 3 antibody . ^^^ These results suggest that 1 ) functional alpha subunit is present in TP serum ; 2 ) intact as well as smaller fragments of IGFBP 3 are present in the big complex of TP serum and are able to bind IGF and complex with alpha subunit ; and 3 ) IGFBP 3 in TP serum has reduced binding to [ 125I ] IGF 1 , but not to [ 125I ] IGF II . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This is mainly due to a decreased production of hepatic insulin like growth factor ( IGF ) 1 and an accumulation of its binding protein ( IGFBP 3 ) , both resulting in a low concentration of free active IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There is evidence that hepatic IGF 1 production is low , whereas the serum concentration of IGF binding protein 3 ( IGF BP 3 ) and other IGFBPs is increased because of the reduced renal clearance of the low molecular weight fragment of IGF BP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Evidence of clinical correlation exists regarding GH status , IGF 1 levels , and the IGFBPs particularly IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both of these BPs were precipitated by anti BP 53 antiserum , but not by anti BP 25 antisera , and both BPs were deglycosylated by endoglycosidase F to a single 31 kD protein which retained the ability to bind IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A RIA is described for the acid labile ( alpha ) subunit of the high mol wt insulin like growth factor ( IGF ) binding protein complex , a glycoprotein of approximately 85 , 000 daltons ( approximately 85K ) which combines with the GH dependent binding protein ( BP 53 or IGFBP 3 ) and IGF 1 or IGF 2 to form the complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Decreased serum IGF 1 concentrations during pregnancy , therefore , may result from reduced IGFBP 3 concentrations causing increased IGF 1 clearance . ^^^ The faster serum elimination half life may result from diminished IGFBP 3 , leading to greater IGF 1 availability to tissues in pregnancy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Like GH , IGF 1 administration raises IGF 1 and two glycosylation variants of IGFBP 3 in the large complex , but unlike GH drastically reduces IGF 2 . ^^^ During IGF 1 infusion , IGFBP 3 appears in the small complex whose IGFBP 2 and IGF 1 increase three to fivefold and fivefold , respectively . ^^^ Conclusions : low GH and / or insulin during IGF 1 infusion and in extrapancreatic tumor hypoglycemia enhance expression of IGFBP 2 and favor partition of IGFBP 3 into the small complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Hypophysectomy significantly decreased IGFBP 3 , IGF 1 , and IGF 2 in nonpregnant mouse serum , increased the amount of IGFBP 2 found in pregnant mouse serum , and had no effect on IGF 1 or 2 concentration in day 14 pregnant mouse serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The 140 kDa insulin like growth factor ( IGF ) binding protein complex in human serum consists of three subunits : an acid labile , non IGF binding glycoprotein ( alpha subunit ) , an IGF binding glycoprotein known as BP 53 or IGFBP 3 ( beta subunit ) , and IGF 1 or IGF 2 ( gamma subunit ) . ^^^ This study investigates the regulation , by salt and glycosaminoglycans , of ternary ( alpha beta gamma ) complex formation , measured by incubating radioiodinated alpha subunit with a mixture of IGF 1 and IGFBP 3 and precipitating bound radioactivity with an anti IGFBP 3 antiserum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , IGF 1 increases the concentrations of IGFBP 3 with the concentration required for half maximal stimulation , approximately 20 ng / ml . ^^^ The opposing effects of FSH and IGF 1 on Sertoli cell IGFBP 3 expression suggests a mechanism by which the IGF 1 biological activity on Sertoli cell might be influenced . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ligand blotting using [ 125I ] IGF 1 revealed a major IGFBP of 40 , 000 mol wt , and treatment of the cells with tunicamycin reduced the mol wt of this protein to about 32 , 000 . mRNA from Swiss 3T3 cells hybridized to a 32P labeled oligonucleotide ( 50 mer ) complementary to rat IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Virtually all circulating insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) are bound to specific binding proteins ( IGFBP ) , of which IGFBP 3 is the quantitatively most important . ^^^ We therefore evaluated the temporal association of serum IGF 1 , IGF 2 , and IGFBP 3 measured by RIAs after well defined short term GH exposure in GH deficient patients . ^^^ The molar ratio of serum IGF 1 plus IGF 2 : serum IGFBP 3 remained constant with values between 0 . 8 0 . 9 except in the constant infusion experiment , in which the ratio increased significantly with time reaching a mean peak value , which exceeded 1 . 0 , after 24 h . ^^^ The earlier increase in serum IGF 1 compared to IGF 2 and IGFBP 3 suggests that IGF 1 may be the main regulator of IGFBP 3 production . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Coincubation of fibroblasts with TGF beta and either IGF 1 or IGF 2 at 50 ng / ml enhanced IGFBP 3 production 1 . 5 to 2 fold compared to TGF beta alone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have examined the developmental pattern of IGF binding protein 1 ( IGFBP 1 ) , 2 , and 3 in serum by Western ligand blotting , the levels of IGFBP 3 , IGF 1 , and IGF 2 in serum by RIA , and the IGFBP mRNA levels of IGFBP 1 , 2 , and 3 in the livers of rhesus monkeys from fetal life through adulthood by Northern analysis . ^^^ The serum levels of IGF 1 and IGF 2 were strongly correlated with the level of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The possibility that sodium dodecyl sulfate ( SDS ) stable complexes of insulin like growth factor 1 ( IGF 1 ) and its binding proteins ( IGF BP ) exist in rat serum has been examined by using SDS polyacrylamide gel electrophoresis ( PAGE ) followed by both [ 125I ] IGF 1 ligand blotting and immunoblotting with antisera directed against either IGF BP 3 or IGF 1 . ^^^ While ligand blotting of rat serum only revealed free IGF BP subunits ( Mr approximately 50 , 35 , and 30 kDa ) , immunoblotting with either the IGF BP 3 antiserum or IGF 1 antiserum revealed major immunoreactive bands with higher molecular weights ( greater than 110 , approximately 100 , and approximately 84 kDa ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 tracer bound to pure rat IGFBP 3 was converted from approx . 60 kDa to 150 kDa by serum alpha subunit , whole rat serum or rat hepatocyte culture medium ; this converting activity was destroyed by transient acidification . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of B 104 cells with IGF 1 increased the expression of both the 24K and 28K IGFBPs and also resulted in the appearance of IGFBP 3 and an unknown IGFBP at 29K . ^^^ Similar to IGF 1 , IGF 2 treatment increased cell number and resulted in the appearance of IGFBP 3 and the 29K IGFBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the effect of purified bovine IGFBP 3 on IGF 1 binding and IGF 1 stimulation of amino acid uptake and DNA synthesis in cultured bovine fibroblasts , a cell culture system highly suitable for these types of studies . ^^^ Preincubation with the IGF 1 analog [ QAYL ] IGF 1 ( 4 nM ) or with 100 nM insulin , growth factors that bind and activate type 1 IGF receptor signalling but have little or no affinity for IGFBP 3 , had effects comparable to IGF 1 , decreasing both IGF 1 binding and action 50 95 % . ^^^ The addition of IGFBP 3 during the preincubation period with IGF 1 blocked the decrease in receptor availability and prevented the cells from becoming desensitized . ^^^ IGFBP 3 did not prevent the [ QAYL ] IGF 1 or insulin induced receptor loss and cellular resistance to IGF 1 . ^^^ These data indicate that IGFBP 3 can prevent IGF 1 induced receptor down regulation , a process that renders cells refractory to further stimulation by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The addition of increasing concentrations of unlabeled IGF 1 in the presence of each IGFBP showed that either IGFBP 1 or IGFBP 3 , but not IGFBP 2 , resulted in a paradoxical increase in [ 125I ] IGF 1 binding to the cell surface . ^^^ In a physiological salt solution , the order of affinity of the IGFBPs for IGF 1 was IGFBP 3 greater than IGFBP 1 greater than IGFBP 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH deficient mice showed a 15 . 7 fold decrease in serum IGF 1 and a 5 . 5 fold decrease in serum IGFBP 3 , but no change in a serum doublet band of 29 , 000 to 34 , 000 Mr , as assessed by ligand blotting . ^^^ When IGF 1 was expressed in the GH deficient mice , serum levels of IGF 1 and IGFBP 3 were 69 % and 64 % of those in normal sera , respectively . ^^^ In mice with IGF 1 overexpression , serum IGFBP 3 was increased 2 . 1 fold and the sum of the 29 , 000 to 34 , 000 doublet bands was increased 2 . 9 fold . ^^^ The results show that IGF 1 can induce IGFBP 3 and IGFBP 2 independently of GH and that IGF 1 is a major controller of these binding proteins . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
B chain IGF 1 ( decreased affinity for IGFBP ) increased cell number and enhanced forskolin ' s effects on IGFBP 3 secretion and mRNA abundance to the same extent as insulin , whereas [ Leu 24 , 1 62 ] IGF 1 ( decreased affinity for the type 1 IGF receptor ) did not . ^^^ These results are in direct contrast to studies with human fibroblasts in which IGF 1 and [ Leu 24 , 1 62 ] IGF 1 stimulate IGFBP 3 secretion , whereas B chain IGF 1 has only a minimal effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 stimulated the secretion of IGFBP 3 from both cell types and IGFBP 2 from HEC 1B cells but either decreased or caused no change in secretion of IGFBP 1 and a 24 , 000 Mr form . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The pretreatment elevation of IGF binding protein 1 decreased by almost 50 % during GH therapy , while IGF binding protein 3 increased significantly in concentration , although this increase was significantly smaller than the GH induced increase in IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Intact but not truncated insulin like growth factor binding protein 3 ( IGFBP 3 ) blocks IGF 1 induced stimulation of osteoblasts : control of IGF signalling to bone cells by IGFBP 3 specific proteolysis . ^^^ Intact but not truncated IGFBP 3 blocked IGF 1 stimulated DNA and glycogen synthesis . ^^^ Inhibition was dose dependent and found whenever the concentration of intact IGFBP 3 exceeded the concentration of IGF 1 . ^^^ The loss of inhibition by IGFBP 3 may be regulated at the site of IGF target cells and thus be essential for IGF 1 induced osteoblast growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although low by RIA , alpha subunit activity in a ternary complex formation assay was normal , indicating that the ability of free alpha subunit in the patients ' circulation to combine with exogenous IGFBP 3 plus IGF 1 was not impaired . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with IGF 1 markedly stimulated the appearance of IGFBP 3 ( 42 / 38 kD doublet ) , a 36 kD IGFBP , and 28 32 kD IGFBPs in the medium of these cells , as assessed by Western ligand blotting ; IGF 1 decreased levels of 24 kD IGFBP in normal HF cultures . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The fetal expression pattern of high IGF 2 and IGFBP 2 is replaced by the adult pattern of low levels of IGF 2 and IGFBP 2 and high levels of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Regression analyses demonstrated that serum IGF 1 was positively correlated with serum IGFBP 3 ( r 2 = 0 . 73 ; P = 0 . 001 ) , whereas serum IGFBP 3 ( r 2 = 0 . 85 ; P = 0 . 001 ) and serum IGF 1 ( r 2 = 0 . 78 ; P = 0 . 001 ) were negatively correlated with serum protease activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since IGFBP 3 must bind IGF 1 or IGF 2 to form a complex with the acid labile subunit ( alpha subunit ) , we have used ternary complex formation from [ 125I ] alpha subunit as a measure of IGF binding to serum IGFBP 3 . ^^^ High pressure liquid chromatography fractions containing IGFBP 3 from pregnancy serum did not bind [ 125I ] IGF 1 , although the equivalent fractions from nonpregnancy serum showed dose dependent binding . ^^^ In contrast , IGFBP 3 fractions from nonpregnancy and pregnancy sera both bound [ 125I ] alpha subunit in the presence of either exogenous IGF 1 or endogenous serum IGFs , implying that non iodinated IGFs could bind to the IGFBP 3 . ^^^ Substitution of nonradioactive iodo IGF 1 for native IGF 1 in the complex formation assay confirmed that the pregnancy induced alteration in IGFBP 3 , probably resulting from proteolysis , prevents it from binding iodo IGF 1 while having little effect on its binding of the native peptide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study was undertaken to investigate the presence of IGF 1 and IGF 2 , as well as that of their binding proteins ( BP ) , IGFBP 1 and IGFBP 3 in human serum and follicular fluid ( FF ) . ^^^ The IGFBP 1 level was found to be significantly higher ( P less than 0 . 01 ) in FF than in serum , whereas IGF 1 and IGFBP 3 values remained markedly lower ( P less than 0 . 01 ) in FF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The two glycosylated forms of IGFBP 3 correlated with increased levels of IGF 1 ( 40 . 5 kD , r = 0 . 468 , p < or = 0 . 01 and 36 . 5 kD , r = 0 . 809 , p < or = 0 . 001 ) , but did not relate to mean GH levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that growth hormone ( GH ) insensitivity is responsible , amongst other mechanisms , for impaired growth in uraemic children , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 were measured by radioimmunoassay in normal control children , in patients with end stage renal failure ( n = 51 ) and in patients with preterminal chronic renal failure ( n = 11 ) and the production rate of IGF was calculated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF binding protein 3 ( IGFBP 3 ) , the principal carrier of IGF 1 in the serum at this age , is decreased by 34 % in protein restricted animals and restored to normal by IGF 1 infusion . ^^^ Contrary to the selective effects of IGF 1 on growth of protein restricted rats , well nourished hypophysectomized rats infused with 150 micrograms / day recombinant human IGF 1 showed a significant growth response , including carcass and organ growth and normalization of IGFBP 3 values . ^^^ We conclude that : 1 ) dietary protein restriction causes organ specific resistance to the growth promoting properties of exogenous IGF 1 ; 2 ) IGF 1 mediates the stimulatory effects of growth hormone on IGFBP 3 synthesis ; and 3 ) the absence of carcass growth in the presence of normal serum IGF 1 concentrations during infusion of IGF 1 suggests that the growth arrest that accompanies protein restriction is mediated in part by resistance to IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that the 28K IGF binding protein from amniotic fluid , like the previously purified 53K binding protein , has high affinity for both IGF 1 and IGF 2 , that it coexists in amniotic fluid with BP 53 or a related protein , and that the levels of both proteins decline with increasing fetal maturity . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
ALS activity was quantitated by the conversion of covalent BP 53 [ 125I ] IGF 1 tracer from about 60K to about 150K . ^^^ In an assay involving competition between test substances and BP 53 IGF 1 tracer in the reaction with partially purified ALS , serum samples , acidified to inactivate endogenous ALS , reacted with potencies proportional to their immunoreactive BP 53 content . ^^^ Pure BP 53 alone was inactive , but after preincubation with IGF 1 or IGF 2 , competed with a potency identical to that of BP 53 in acidified serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The proteins tested in reconstitution mixtures were an acid labile Mr 84 , 000 86 , 000 glycoprotein doublet ( alpha subunit ) , an acid stable Mr 47 , 000 53 , 000 glycoprotein doublet with IGF binding activity ( BP 53 or beta subunit ) , and IGF 1 or IGF 2 ( gamma subunit ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Comparison of IGF 1 and IGF 2 binding by fibroblast BP 53 revealed slightly higher IGF 2 than IGF 1 binding , and association constants of 3 4 10 10 ( 10 ) liter / mol for both IGFs , similar to BP 53 from human plasma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study investigates the effects of BP 53 , the acid stable IGF binding subunit of the circulating 150 kDa IGF binding protein complex , on IGF 1 stimulated thymidine incorporation by neonatal human skin fibroblasts . ^^^ Co incubation of IGF 1 with increasing concentrations of pure BP 53 caused dose dependent inhibition of IGF 1 stimulated thymidine incorporation , which was complete when IGF 1 and BP 53 were equimolar . ^^^ In contrast , preincubation of cells with BP 53 for 8 48 h before adding IGF 1 resulted in a potentiation of the subsequent IGF 1 effect . ^^^ The potentiation was maximal ( 2 fold ) at a BP 53 concentration of 150 ng / ml , and appeared to act by increasing the maximal rate of thymidine incorporation rather than the sensitivity of this process to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have investigated the relative diagnostic merits of measuring ( 1 ) peak GH response to insulin induced hypoglycemia ( ITT ) , ( 2 ) mean 24 hour GH concentration derived from 20 minute sampling ( IGHC ) , ( 3 ) serum IGF 1 levels , and ( 4 ) serum insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) levels . ^^^ There was a significant overlap of IGF 1 and IGFBP 3 values , with only a third of GH deficient subjects having low IGF 1 values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum from Zn deficient animals labeled in vitro with [ 125I ] IGF 1 displayed three peaks of tracer activity : 150 kd ( IGFBP 3 ) , 37 kd ( IGFBP 2 and 1 ) , and 8 kd ( free [ 125I ] IGF 1 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For this reason we have studied the effect of long term theophylline administration on 16 laboratory parameters concerning the metabolism of proteins , glucose , lipids , hormones and the glomerular function ( blood : hemoglobin , glucose , albumin , prealbumin , urea nitrogen , creatinine , cholesterol , triglycerides , apolipoproteins A 1 and B 100 , IGF 1 , IGFBP 3 ; urine : urea nitrogen , creatinine , C peptide , GH ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition of rhIGFBP 3 to conditioned media inhibited 125I rhIGFBP 4 proteolysis by 90 % , while IGF 1 and IGF 2 reversed the inhibitory effects of rhIGFBP 3 in a dose dependent manner . 125I rhIGFBP 4 proteolysis was not inhibited by N terminal rhIGFBP 3 fragments that bind IGFs , but was inhibited by two synthetic peptides corresponding to sequences contained in the mid region or C terminal region of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 tracer , monoiodinated on Tyr 31 , discriminated less than other tracers between intact and proteolysed IGFBP 3 . ^^^ In competitive binding curves using this tracer , intact IGFBP 3 showed comparable reactivity towards IGF 1 and analogs with substitutions at Tyr 24 , Tyr 31 or Tyr 60 . ^^^ In contrast , proteolysed IGFBP 3 reacted equally with IGF 1 and [ Ala 31 ] IGF 1 ( approximately 10 fold lower potency than with intact IGFBP 3 ) , but showed a marked selectivity against [ Ser 24 ] IGF 1 and [ Leu 60 ] IGF 1 ( approximately 100 fold lower potency than with intact IGFBP 3 ) . ^^^ The affinity of IGF 1 binding to proteolysed , but not intact , IGFBP 3 was increased by the addition of the acid labile subunit of the IGFBP 3 complex . ^^^ This study defines more fully the lesion in IGFBP 3 caused by serum proteolysis during pregnancy and demonstrates that tyrosine substituted IGF 1 derivatives are valuable tools in studying IGFBP 3 proteolysis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study compared levels of the IGF 1 and IGF 2 , and of the IGF binding protein 2 ( IGFBP 2 ) and IGFBP 3 in vitreous from three groups with different degrees of retinal ischemia , as judged by the extent of neovascularization : a control group without new vessel formation , retinal neovascularization in patients with proliferative diabetic retinopathy , and massive ischemia of various causes resulting in rubeosis . ^^^ IGF 1 and IGFBP 3 were increased 10 and 13 fold in rubeosis ( P < < 0 . 01 ) compared with no ischemia ( n = 10 ) , while IGF 2 and IGFBP 2 were elevated 2 . 7 and 4 . 3 fold ( P < 0 . 01 ) . ^^^ Vitreous from patients with proliferative diabetic retinopathy but without rubeosis ( n = 16 ) contained 2 . 5 and 2 . 2 fold elevated levels of IGF 1 and of IGFBP 2 ( P < 0 . 05 ) , while IGF 2 and IGFBP 3 were increased 1 . 4 and 1 . 6 fold , which was not significant . ^^^ We conclude that : ( a ) ischemia appears to be a strong stimulus for the local production of IGF 1 and 2 and of IGFBP 2 and 3 in the eye . ( b ) Changes in IGF 1 and IGFBP 2 in proliferative diabetic retinopathy may be secondary to local ischemia rather than being specific for diabetic retinopathy . ( c ) IGF 1 and IGFBP 3 may play a role in mediating angioneogenesis in the eye . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The mammogenic growth factors , IGF 1 ( 13 . 3 nM ) and epidermal growth factor ( EGF ; 1 . 7 nM ) , both stimulated DNA synthesis ( P < 0 . 05 ) ; however , their effects on IGFBP 3 secretion differed . ^^^ IGF 1 stimulated IGFBP 3 secretion whereas EGF was inhibitory . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with IGF 1 , [ QAYL ] IGF 1 , or alpha IR 3 had no effect on steady state levels of the 2 . 5 kb IGFBP 3 or the 1 . 3 kb IGFBP 6 mRNA transcripts as measured by Northern blotting and quantitative autoradiography . ^^^ IGF 1 , forskolin , and the candidate growth factors had no effect on either IGFBP 3 or IGFBP 6 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously demonstrated that retinoic acid ( RA ) inhibition of IGF 1 stimulated MCF 7 cell proliferation is associated with increased IGFBP 3 levels in the conditioned media . ^^^ We therefore investigated the effect of recombinant IGFBP 3 as well as IGFBP 2 , 4 and 5 on IGF 1 stimulation of DNA synthesis and IGF 1 binding in the MCF 7 human breast carcinoma cell line . ^^^ Transfection of MCF 7 cells with an IGFBP 3 expression vector resulted in the enhanced secretion of IGFBP 3 with an accompanying increase in IGF 1 binding as well as increased cell proliferation upon treatment of the cells with IGF 1 . ^^^ IGF 1 preincubation of MCF 7 cells transfected with control pSVneo plasmids results in cells refractory to further IGF 1 stimulation of thymidine incorporation while IGF 1 continues to stimulate [ 3H ] thymidine incorporation in IGFBP 3 transfected MCF 7 cells , suggesting that IGFBP 3 protects the cells from IGF 1 mediated down regulation of its receptor . ^^^ Therefore , IGFBP 3 secreted by MCF 7 cells can enhance IGF 1 stimulation of DNA synthesis , increase IGF 1 binding to these cells , and prevent IGF 1 induced desensitization of its own receptor , suggesting that IGFBP 3 plays a significant role in IGF 1 mediated breast carcinoma proliferation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We speculate that an increase in IGF 1 concentrations , normalization of IGFBP levels , and a decrease in IGFBP 3 proteolytic activity in serum may all be involved in the improved recovery and catch up growth observed with the high protein diet . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously reported that adult GH receptor deficient ( GHRD ) patients treated subcutaneously with recombinant human insulin like growth factor ( IGF ) 1 have increased serum IGF 1 levels and decreased IGF 2 levels , whereas IGF binding protein 3 ( IGFBP 3 ) levels were unchanged . ^^^ The composition and distribution of insulin like growth factors ( IGFs ) and IGF binding proteins ( IGFBPs ) in the serum of growth hormone receptor deficient patients : effects of IGF 1 therapy on IGFBP 3 . ^^^ To further investigate the effects of IGF 1 administration upon the IGF IGFBP axis in GHRD , we have examined : 1 ) the molecular distribution of IGF 1 and IGF 2 among the IGFBPs ; 2 ) the composition and distribution of the IGFBPs , in particular IGFBP 3 ; and 3 ) the acid labile subunit ( ALS ) . ^^^ Administration of IGF 1 to GHRD patients was unable to increase concentrations of the molecular forms of IGFBP 3 , correct the aberrant distribution of IGFs among the IGFBPs , or increase serum concentrations of ALS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Most insulin like growth factor 1 ( IGF 1 ) in blood is found in complex with IGF binding protein 3 ( IGFBP 3 ) . ^^^ However , it is not clear whether IGFBP 3 which is not complexed to acid labile subunit can gain access to tissues and contribute to the transcapillary transport of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 exhibited significant correlations with BW , PI , and PW , similar to those seen with IGF 1 . ^^^ IGF 1 levels correlated strongly with IGFBP 3 levels ( r = 0 . 646 , P = 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The decreased synthesis of the autocrine / paracrine growth factor IGF 1 together with decreased levels of a positive modulator IGFBP 3 , and the increased levels of a negative modulator IGFBP 4 in the extracellular milieu , may be responsible for the reduced proliferative capacity in cells expressing abundant connexin43 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Synthesis of IGFBP 3 is stimulated by IGF 1 and attenuated by gonadotropin . ^^^ Additionally , IGF 1 attenuation of IGFBP 3 degradation was not inhibited by antagonism of receptor action with a tyrosine kinase inhibitor . ^^^ However , long R 3 IGF 1 attenuated IGFBP 3 degradation even though it has low affinity for IGFBPs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of high protein feed supplements on concentrations of growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in plasma and on the amounts of GH and messenger RNA for GH in the pituitary glands of adult rams . ^^^ Serial blood samples were taken at 15 min intervals for 12 h for the determination of GH and IGF 1 content by radioimmunoassay and for IGF binding protein 3 ( IGFBP 3 ) levels by Western blotting . ^^^ In conclusion , a high protein diet decreases plasma GH levels and increases IGF 1 without changing plasma IGFBP 3 levels in rams . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors 1 ( IGF 1 ) and 2 ( IGF 2 ) and their binding proteins ( IGFBP 1 , IGFBP 2 and IGFBP 3 ) were measured by radioimmunoassay in plasma and milk samples collected throughout the study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of the GH dependent peptides , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) were also measured . ^^^ Despite higher GH levels in the CFDM patients , their IGF 1 and IGFBP 3 concentrations were low . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE We assessed the relationship between serum IGFBP 3 levels with IGF 1 and quantitative GH secretory status in poorly growing children . ^^^ DESIGN We studied the relationship between 24 hour integrated concentration of GH , peak GH to paired sequential stimulation tests , IGF 1 and the IGFBP 3 serum levels . ^^^ GH , IGFBP 3 and IGF 1 were measured by radioimmunoassay . ^^^ RESULTS IGFBP 3 SDS was strongly correlated with IGF 1 SDS ( r = 0 . 64 , P < 0 . 0001 ) , and weakly with peak GH ( r = 0 . 28 , P < 0 . 0004 ) , but not with the integrated GH concentration ( r = 0 . 07 , P < 0 . 46 ) . ^^^ CONCLUSION In short children , IGFBP 3 levels increase with puberty , are strongly correlated with IGF 1 levels , weakly correlated with peak response to GH stimulation tests , but not correlated with integrated GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These patients also underwent arginine infusion and L dopa stimulation tests and had measurements of morning baseline GH binding protein ( GHBP ) , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) plasma concentrations . ^^^ In spite of these findings , plasma concentrations of IGF 1 , IGFBP 3 , and GHBP were within the normal range in these patients . ^^^ In these patients , plasma IGF 1 and GHBP remained normal , whereas IGFBP 3 decreased significantly , albeit within the normal range . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) , when occupied by IGF 1 or IGF 2 , combines with an acid labile glycoprotein subunit ( ALS ) to form a high molecular weight complex . ^^^ Purification involved ion exchange chromatography , and affinity chromatography on an IGF 1 IGFBP 3 column , yielding almost 1 mg ALS from 100 ml serum . ^^^ Rat ALS was almost as potent as human ALS in binding to human IGF 1 IGFBP 3 complex ( association constant , 2 . 3 nM 1 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Critically ill , septic patients have high basal levels of GH , low levels of IGF 1 and its carrier binding protein IGFBP 3 , high levels of an inhibitory binding protein , IGFBP 1 , and increased serum protease activity which reduces the affinity of IGFBP 3 for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 binding activity of 38 41 kDa species ( corresponding to IGFBP 3 ) was not different between extracts of cancer tissue and those of normal tissue , whereas that of 28 32 kDa species was significantly higher in cancer tissue extracts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Repeated administration of IGF 1 decreased IGF binding protein 3 levels , producing lower intrainfusion levels of IGF 1 and limiting its therapeutic efficacy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This occurs without disrupting the 150 kilodalton ( kDa ) complexes in which IGFBP 3 carries of most of the IGF 1 and IGF 2 in serum . ^^^ Nevertheless , competitive binding experiments using [ 125I ] IGF 1 and 2 and IGFBP 3 preparations from each of the plasma pools showed that the competitive potencies of IGF 2 and especially IGF 1 were lower in pregnancy IGFBP 3 than in normal IGFBP 3 . ^^^ Scatchard analysis revealed a 2 fold loss of affinity for IGF 2 and a 10 fold loss for IGF 1 compared with that for normal plasma IGFBP 3 . ^^^ In studies at 37 C of the kinetics of dissociation of [ 125I ] IGF 1 and 2 bound to IGFBP 3 , IGF 2 was dissociated 6 times faster , and IGF 1 10 times faster from pregnancy plasma IGFBP 3 than from normal plasma IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These results show that despite low GH secretion , genetically obese Zucker rats have 1 ) normal linear ( nasoanal ) growth , 2 ) normal or increased circulating levels of IGF 1 and IGFBP 3 , and 3 ) increased plasma IGF 1 responses to exogenous GH . ^^^ These results suggest that the GH independent growth in this model could result from direct effects of hyperinsulinism on circulating IGF 1 and IGFBP 3 levels and / or indirect effects through increased GH receptor function . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Six months after irradiation and during chemotherapy there was a transient decline in IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and GH BP values ( respective mean values of 56 . 1 + / 9 . 0 ng / mL , 1 . 1 + / 0 . 2 microgram / mL , and 7 . 6 + / 3 . 3 % of radioactivity as compared to time 0 values : 13 % % o / 15 ng / mL , 2 . 2 + / 0 . 2 micrograms / mL , and 20 . 0 + / 4 . 0 % , P < 0 . 001 ) , although provoked GH secretion was normal at this time . ^^^ The IGF 1 , IGFBP 3 , and GH BP returned to pretreatment ranges by 12 36 months after initiation of the study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Other effects caused specifically by IGF 1 , but not testosterone , included increases in serum IGFBP 3 , body weight and spleen weight . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 also evoked a concentration dependent increase in the abundance of IGFBP 3 in confluent , but not sparse , SMCs without affecting IGFBP 3 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The impact of obesity , fat distribution , and energy restriction on insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , insulin , and growth hormone . ^^^ In 51 females and nine males having a body mass index ( BMI ) between 27 and 39 kg / m2 , relationships between serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , insulin , growth hormone ( GH ) , blood glucose , and anthropometric measurements of body fat were examined . ^^^ Both serum IGF 1 and insulin levels were positively correlated with serum IGFBP 3 levels ( r = . 34 , P = . 009 and r = . 34 , P = . 008 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 and IGFBP 3 ( determined by ligand blot analysis ) in FFL from both DOM and SUB were lower ( p < 0 . 05 ) in GRFi than in HSAi cows . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Expression and secretion of IGFBP 3 were hormonally responsive and strongly correlated ( r = 0 . 79 ; P < 0 . 001 ) , with 2 to 3 fold stimulation by added insulin or IGF 1 ( both P < 0 . 05 ) , but not by added GH alone . ^^^ Our findings suggest that GH may act indirectly to promote IGFBP 3 generation in vivo via increasing both the secretion of insulin and the hepatic production of IGF 1 ; in patients with diabetes mellitus , reduced circulating levels of IGFBP 3 despite high levels of GH may result from both insulin deficiency and inadequate hepatic production of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Administration of growth hormone ( GH ) , but not insulin like growth factor 1 ( IGF 1 ) , by continuous infusion can induce the formation of the 150 kilodalton IGF binding protein 3 complex in GH deficient rats . ^^^ Serum IGF 1 and IGFBP 3 levels were less than 10 % of those in normal rats . ^^^ Administration of GH twice daily did not increase serum IGF 1 or IGFBP 3 concentrations , and the molecular distribution of IGFBP 3 remained unchanged . ^^^ In contrast , continuous infusion of GH resulted in 5 fold increases in serum IGF 1 and increases in IGFBP 3 levels . ^^^ Thus , GH infusion was able to induce formation of the 150 kDa ternary complex by increasing circulating levels of IGF 1 , IGFBP 3 , and presumably ALS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study we have 1 ) assessed how differences in insulin and GH status between obese patients with noninsulin dependent diabetes mellitus ( NIDDM ) and healthy obese ( OB ) and nonobese ( NOB ) subjects are associated with different responses of insulin like growth factors ( IGFs ) and IGF binding proteins ( IGFBPs ) to fasting , and 2 ) determined whether the IGF 1 response to fasting in healthy subjects is secondary to changes in IGFBP 3 . ^^^ IGFBP 3 , the major determinant of the IGF 1 turnover rate in serum , was unchanged by fasting , as determined by RIA and Western ligand blot analysis . ^^^ In conclusion , 1 ) differences in GH status and modulation of GH induction of IGF 1 by insulin resistance could contribute to low basal IGF 1 levels and lack of a IGF 1 response to fasting in patients with NIDDM ; and 2 ) the turnover rate of IGF 1 in serum , which is largely determined by IGFBP 3 , is not likely to be altered by short term fasting , suggesting that the decrease in serum IGF 1 concentrations is a result of decreased IGF 1 production . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We tested the hypothesis that bioactive GH ( B GH ) concentrations would correlate better with the GH dependent peptides , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) than would GH determined by conventional RIA ( RIA GH ) . ^^^ For each girl , an equal aliquot of each overnight sample was pooled for determination of B GH , RIA GH , IGF 1 , IGFBP 3 , LH , FSH , and estradiol . ^^^ Measurable estradiol concentrations were present in six girls and were sufficient to suppress gonadotropin concentrations in two girls , but they did not alter B GH , RIA GH , IGF 1 , and IGFBP 3 concentrations compared with the age matched girls without measurable estradiol concentrations . ^^^ RIA GH did not correlate significantly with B GH , IGF 1 , or IGFBP 3 . ^^^ B GH exhibited a significant correlation with IGF 1 ( r = 0 . 407 , p < 0 . 05 ) , and the correlation with IGFBP 3 was better than that for RIA GH ( r = 0 . 355 versus 0 . 064 , B GH and RIA GH , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When milk production had ceased , as determined by litter weight loss and the absence of milk in the stomachs of pups , attempts were made to reinitiate lactation using prolactin , GH , insulin like growth factor 1 ( IGF 1 ) precomplexed to recombinant human IGF binding protein 3 ( hIGFBP 3 ) or IGF 1 plus IGF 2 precomplexed to hIGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) in serum is predominantly bound in a ternary complex , consisting of IGF peptide , IGF binding protein 3 ( IGFBP 3 ) , and an acid labile subunit , or a binary complex , consisting of IGF peptide and any of the six IGFBPs . ^^^ Proteolysis of IGFBP 3 may alter the distribution of IGF 1 between these complexes by reducing IGFBP 3 affinity for IGF 1 and / or acid labile subunit and may offer an additional mechanism for regulation of IGF availability . ^^^ Further studies are required to determine whether the avidity of ternary complex formation with the 29 kDa IGFBP 3 fragment is reduced and whether the resulting increased IGF turnover can explain the reduced IGF 1 levels ( z scores ) observed in NIDDM patients compared to healthy subjects ( 0 . 81 + / 0 . 32 SD vs . +0 . 26 + / 0 . 17 SD ; P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report ( 1 ) an increase in serum IGF 2 concentrations ( about 2 fold ) , whereas IGF 1 concentrations are regarded as normal when aging is taken into account ; ( 2 ) an apparent increase in serum IGFBP 3 levels when compared to those of healthy elderly subjects , IGFBP 3 only being detected in the 150 kDa IGFBP ternary complex as in normal serum ; ( 3 ) abnormally elevated serum IGFBP 2 levels taking into account the apparent concentrations of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Notwithstanding these differences , the two IGF 1 variants showed only slight between species binding differences when tested with purified rat , sheep and human IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In order to assess if the anabolic action of PTH is related to changes in circulating levels of insulin like growth factor 1 and 2 ( IGF 1 and 2 ) , and IGF binding protein 3 ( IGFBP 3 ) , 24 h of PTH infusion was performed in healthy women and in patients with rheumatoid arthritis ( RA ) , a state where both bone metabolism and PTH secretion is influenced by the inflammatory activity . ^^^ IGFBP 3 was not affected by PTH infusion in patients with RA when the disease had high activity , but when inflammation had subsided they responded with a similar increase in IGFBP 3 as the control group and basal IGF 1 and 2 levels were normalised . ^^^ Since IGFBP 3 can enhance the anabolic action of IGF 1 , increased IGFBP 3 levels after PTH infusion may reflect a mechanism by which PTH is anabolic for bone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After each treatment period ( 4 weeks ) , serum profiles of GH , IGF 1 , IGF binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) , glucose , insulin , non esterified fatty acids ( NEFA ) , glycerol , 3 hydroxybutyrate , alanine , lactate and glucagon were measured for 37 hours after GH injection ( 3 IU / m2 at 1900 hour ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( BP 3 ) are two products involved in mediating the growth promoting actions of GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During each treatment period , concentrations of androgens , lipids , apolipoproteins , IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , insulin sensitivity , percent body fat , libido , and sense of well being were measured . ^^^ Although mean 24 h GH and IGFBP 3 levels were unchanged , serum IGF 1 levels increased significantly , and IGFBP 1 decreased significantly for both genders , suggesting an increased bioavailability of IGF 1 to target tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The data reveal a negative correlation between uterine weight and uterine IGFBP 3 gene expression as well as reciprocal regulation by estradiol of expression in uterus of the genes encoding IGF 1 and IGFBP 3 . ^^^ Together , these observations provide a novel mechanism by which estradiol and antiestrogens modulate uterine IGF 1 physiology that is consistent with the view that the mitogenic activity of IGF 1 is reduced in the presence of IGFBP 3 . ^^^ The uterotrophic toxicity of chronic estradiol or tamoxifen treatment may be causally related to both the inhibition of uterine IGFBP 3 expression and the stimulation of uterine IGF 1 expression by these compounds . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have studied the physiology and tissue expression of IGF 1 and IGF BP 3 in pregnant and lactating rats . ^^^ IGF 1 and IGF BP 3 expression levels were determined in mammary gland and liver by slot blotting . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Further , the quality of embryos , development of the endometrium , rate of clinical pregnancy , and serum and follicular fluid ( FF ) concentrations of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) , and IGFBP 3 were estimated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the relation between normal human fetal growth and the levels of insulin like growth factors ( IGF 1 , IGF 2 ) , their receptors , and IGF binding protein 3 ( IGFBP 3 ) in both the maternal and fetal compartments . ^^^ Aliquots of amniotic fluid ( AF ) and serum samples were analyzed for levels of IGF 1 , IGF 2 , and IGFBP 3 . ^^^ There were no significant correlations between newborn weights and IGFBP 3 , or maternal serum levels of IGF 1 and IGF 2 . ^^^ CONCLUSION : These data demonstrate the presence of type 1 receptors and the bioavailability of IGF 1 , IGF 2 , and IGFBP 3 throughout pregnancy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and especially IGF 2 and their major growth hormone dependent binding protein ( IGFBP 3 ) were significantly reduced , although growth hormone secretion after a pharmacological stimulus was normal . ^^^ In vitro studies of fibroblasts derived from hypertrophied tissue showed normal IGF 1 production and somewhat reduced IGF 2 and IGFBP 3 production as compared to normal human skin fibroblasts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Rat Sertoli cells in culture produce insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) is the predominant IGFBP . ^^^ Recombinant nonglycosylated human IGFBP 3 was added to cultured Sertoli cells derived from 15 day old rats , and the change in concentration over time in the medium was measured by [ 125I ] IGF 1 ligand blot analysis and Western blot analysis using an antiserum specific for human IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Response of insulin like growth factor ( IGF ) binding protein 1 ( IGFBP 1 ) and IGFBP 3 to IGF 1 treatment in severe insulin resistance . ^^^ IGFBP 1 concentrations did not significantly change during the infusion but those of IGFBP 3 increased from 1655 + / 127 to 2197 + / 334 micrograms / l ( P = 0 . 002 ) , despite a significant fall in GH concentrations from 10 . 7 + / 2 . 6 to 4 . 1 + / 1 . 1 mU / l ( P = 0 . 007 ) , suggesting that IGFBP 3 regulation is also IGF 1 dependent . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 were compared between 40 postmenopausal breast cancer patients receiving long term tamoxifen therapy and 39 breast cancer patients receiving no hormonal treatment . ^^^ There were no significant differences in the mean concentrations ( + / standard error of the mean ) of IGF 1 ( 10 . 0 + / 0 . 4 nmol / l and 11 . 2 + / 0 . 5 nmol / l , respectively ) and IGFBP 3 ( 3 . 2 + / 0 . 1 mg / l and 3 . 1 + / 0 . 1 mg / l , respectively ) , whereas the mean value of IGFBP 1 was significantly higher in the tamoxifen group ( 6 . 0 + / 0 . 6 micrograms / l versus 2 . 8 + / 0 . 3 micrograms / L , P = 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During food restriction , plasma IGF binding protein 3 ( IGFBP 3 ) and to a lesser extent IGFBP 1 were decreased , and IGFBP 2 was increased ; after switching to ad libitum feeding , plasma levels of IGFBP 2 normalized , but levels of IGFBP 1 and IGFBP 3 remained low in the face of normalized plasma IGF 1 levels . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For partial GHD , among those with low IGF 1 ( N = 19 ) 68 % were also low for IGFBP 3 , while 80 % of those with normal IGF 1 ( N = 30 ) were also normal for IGFBP 3 . ^^^ For these groups , among those with low IGF 1 ( N = 22 ) 73 % also were low for IGFBP 3 , while 80 % of those with normal IGF 1 ( N = 81 ) also were normal for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 area under the curve on days 1 and 7 was correlated to the IGFBP 3 levels . ^^^ Only the patient with the highest IGFBP 3 level obtained IGF 1 levels above 100 micrograms / l for 24 h . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) increased significantly from 100 to 279 micrograms / l and IGF binding protein 3 ( IGFBP 3 ) from 1930 to 3355 micrograms / l after 4 months of GH treatment ( p < 0 . 0001 ) . ^^^ In addition , the molar ratio between IGF 1 and IGFBP 3 increased significantly from 0 . 22 to 0 . 33 after GH treatment ( p < 0 . 0001 ) . ^^^ The GH induced increase in bone alkaline phosphatase correlated significantly with the increase in serum IGFBP 3 ( r = 0 . 46 , p = 0 . 04 ) but not with the increase in serum IGF 1 ( p = 0 . 16 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These results suggest that RA may act to inhibit cervical cell growth by increasing IGFBP 3 levels and reducing the extracellular concentration of free insulin like growth factor 1 ( IGFI ) and / or alternatively , IGFBP 3 may inhibit cell growth by direct effects on the cell , independent of IGFI . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor 1 ( IGF 1 ) levels were similar at baseline ( rhGH , 133 + / 40 . 4 micrograms / L ; placebo , 128 + / 13 ) . rhGH increased IGF 1 and IGF 1 binding protein 3 ( IGFBP 3 ) in all subjects [ 6 month IGF 1 in plus estrogen women , 230 + / 25 . 4 micrograms / L ; in those not receiving estrogen ( minus estrogen ) , 308 + / 21 . 3 ] . ^^^ No changes in IGF 1 or IGFBP 3 occurred with placebo ( IGF 1 , 144 + / 21 . 3 micrograms / L ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of GH , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 were reduced in Ssa females , particularly after menarche . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In man , the 24 hour serum levels of IGF 1 and IGFBP 3 remain constant , but short term changes in the IGF I / IGFBP 3 ratio have been described following GH administration . ^^^ Serum levels of IGF 1 and IGFBP 3 decrease with age in normal adults and are elevated in active acromegaly due to excessive GH secretion . ^^^ However , the individual ratios between serum levels of IGF 1 and IGFBP 3 in acromegalic and healthy adults have not been described previously . ^^^ In all subjects a single blood sample was drawn for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and GH measurements by specific RIAs . ^^^ RESULTS : In healthy adults serum levels of IGF 1 and IGFBP 3 decreased with increasing age ( r = 0 . 52 and r = 0 . 34 , respectively , P < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with IGF 2 increased the level of IGFBP 3 , as did IGF 1 or GH treatment , as shown by Western ligand blotting . ^^^ In conclusion , IGF 1 and IGF 2 as well as GH have a stimulatory effect on general body growth and are effective in the stimulation of serum IGFBP 3 , sulfate incorporation into cartilage , as well as the growth of specific organs in Snell dwarf mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 mRNA is increased in osteoblasts exposed to PTH or to GH but not in response to 5 10 10 ( 9 ) mol / l IGF 1 . ^^^ PTH exerts a rapid ( within 2 h ) stimulatory effect on IGF 1 and IGFBP 3 production , both at the message and peptide levels , whereas GH increases only IGFBP 3 , both at the message and peptide levels ( after 24 h ) . ^^^ We conclude that IGF 1 does not mediate increased IGFBP 3 production by rat osteoblasts in response to GH and PTH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that a dysfunctional growth hormone ( GH ) insulin like growth factor ( IGF ) axis may play a role in the pathogenesis of osteoporosis , we compared the levels of IGF 1 , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) in 15 women with spinal osteoporosis ( i . e . at least one non traumatic vertebral fracture ) and 15 normal age matched women . ^^^ The basal levels of IGF 1 , IGF 2 and IGFBP 3 were similar in patients and controls ( mean + / SEM ) : IGF 1 , 16 . 5 + / 1 . 3 versus 16 . 0 + / 1 . 3 nmol / l ( NS ) ; IGF 2 , 79 . 9 + / 3 . 6 versus 72 . 5 + / 4 . 1 nmol / l ( NS ) ; and IGFBP 3 , 125 . 7 + / 6 . 5 versus 130 . 3 + / 7 . 8 nmol / l ( NS ) . ^^^ Stimulation with r hGH elicited increased levels of IGF 1 , IGF 2 and IGFBP 3 within both groups ( p < 0 . 001 ) . ^^^ The maximal values expressed as a percentage of baseline were : IGF 1 , 341 + / 26 % versus 369 + / 22 % , IGF 2 , 125 + / 4 % versus 119 + / 5 % , IGFBP 3 , 141 + / 5 % versus 147 + / 7 % in osteoporotic patients and controls , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated IGF 1 , IGF 2 , and IGFBP 3 levels by specific RIAs in CSF from patients with central nervous system ( CNS ) tumor or leukemia and compared them with values in patients with meningitis . ^^^ The CSF from 38 children who received lumbar punctures to exclude meningitis was used to define the normal range for IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 protease activity in CSF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Use of insulin like growth factor binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and IGF 1 for assessing growth hormone status in short children . ^^^ IGFBP 2 is regulated differently from IGF 1 and IGFBP 3 , because its serum concentrations are elevated in some adults with GH deficiency ( GHD ) , whereas IGF 1 and IGFBP 3 concentrations are usually decreased . ^^^ The purposes of this study were to define the normal range of IGFBP 2 concentrations in children , to determine its efficacy in the diagnosis of GHD , and to compare the diagnostic value of measurements of the serum GH response to provocative testing with results of measurements of IGFBP 2 , IGFBP 3 , and IGF 1 . ^^^ Of the 7 , all had an increased IGFBP 2 / IGF 1 ratio and a low IGFBP 3 level . ^^^ Of the remaining 16 children with normal IGF 1 , 13 had a normal IGFBP 2 / IGF 1 ratio and normal IGFBP 3 values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of insulin on the hepatic production of insulin like growth factor binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and IGF 1 in insulin dependent diabetes . ^^^ The main source of circulating IGF 1 and IGFBP 1 is considered to be the liver , but that of circulating IGFBP 3 is not known . ^^^ IGF 1 and IGFBP 3 are GH dependent , whereas IGFBP 1 is insulin regulated . ^^^ The aim of the present study was to examine the effect of insulin on the hepatic secretion of IGFBP 1 , IGFBP 3 , and IGF 1 . ^^^ There was a significant correlation in the hepatic vein between fasting IGF 1 and IGFBP 3 levels ( r = 0 . 928 ; P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To establish the major determinants of bone mass , we assessed relationships between bone mineral density ( BMD ) and height , weight , body mass index ( BMI ) , muscle strength , physical capacity ( VO2max ) , body composition , serum concentrations of insulin like growth factor 1 ( IGF 1 ) , growth hormone ( GH ) , the GH dependent IGF binding protein ( IGFBP 3 ) , testosterone , sex hormone binding globulin ( SHBG ) , osteocalcin , and parathyroid hormone ( PTH ) in 38 healthy men between 25 and 59 years of age . ^^^ Values of BMD at all sites ( total body , lumbar spine , and hip ) were strongly correlated with IGFBP 3 ( r = 0 . 51 0 . 64 , p < 0 . 001 at all sites ) , and total body BMD was also significantly correlated with IGF 1 ( r = 0 . 43 , p = 0 . 01 ) . ^^^ IGF 1 and IGFBP 3 were both positively related to muscle strength and VO2max . ^^^ In a stepwise forward multiple regression analysis , the best model was obtained for the femoral neck , where IGFBP 3 , GH , PTH , age , IGF 1 , and BMI explained 77 % of the variation in BMD . ^^^ The partial regression coefficients of IGFBP 3 , PTH , and BMI were all positive , whereas age , GH , and IGF 1 were negatively correlated with BMD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Developmental changes in serum IGF 1 and IGFBP levels and liver IGFBP 3 mRNA expression in intrauterine growth retarded and control swine . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To more clearly document these relations , we designed a randomized , double blind , placebo controlled study of the effects of tamoxifen and continuous estrogen on circulating levels of Lp ( a ) , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) in healthy postmenopausal women . ^^^ Both estrogen and tamoxifen decreased serum levels of IGF 1 to 30 % below baseline during the 3 months of treatment , while IGFBP 3 levels were unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 induced IGFBP 3 potentiates the mitogenic actions of IGF 1 in mammary epithelial MD IGF 1 cells . ^^^ Levels of IGFBP 3 mRNA and secretion of IGFBP 3 by MD IGF 1 cells were stimulated by IGF 1 , insulin ( INS ) , and IGF 1 analogs but not prolactin ( PRL ) . ^^^ Secretion of recombinant IGF 1 caused a 26 . 5 fold increase in secretion of IGFBP 3 , as measured by densitometric analysis of ligand blots , which was associated with a 1 . 7 fold increase in total DNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Enhanced expression of dihydrofolate reductase by bovine kidney epithelial cells results in altered cell morphology , IGF 1 responsiveness , and IGF binding protein 3 expression . ^^^ To determine if cells which were secreting IGFBP 3 had altered growth responses to IGF 1 , cells were grown in serum free media in the presence of IGF 1 ( 0 to 100 ng / ml ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , energy balance appeared to be a major regulator of concentrations of IGF binding protein 3 and responsiveness of IGF 1 to exogenous somatotropin before parturition . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have therefore examined the effects of recombinant human IGF 1 ( rhIGF 1 ) administration on the levels of IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 and IGF bioactivity in a group of 9 late pubertal adolescents with IDDM . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To investigate the pathophysiology of growth retardation in adolescents with anorexia nervosa , we measured basal growth hormone ( GH ) , growth hormone binding protein ( GHBP ) , IGF 1 , and insulin like growth factor binding protein 3 ( IGFBP 3 ) in three groups of patients : ( 1 ) 28 recently hospitalized female adolescents with anorexia nervosa , ( 2 ) 23 of the same patients after partial weight restoration , and ( 3 ) 28 healthy control subjects matched for age , sex , and pubertal stage . ^^^ In contrast , serum GHBP ( p < 0 . 001 ) , IGF 1 ( p < 0 . 001 ) , and IGFBP 3 ( p < 0 . 01 ) were significantly lower in group 1 than in group 3 . ^^^ In group 2 ( after refeeding ) the serum IGF 1 concentration increased significantly and GHBP and IGFBP 3 returned to normal . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Of the total circulating IGF 1 and IGF 2 , 70 % was carried by the 150 kDa complex in normal subjects , while in patients with GHRD , 50 % of serum IGF 1 , but only 30 35 % of serum IGF 2 , was measured within the 150 kDa IGFBP 3 region . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have recently suggested that the bioavailability of circulating IGF 1 is increased in the human fetus due to the molar excess of IGF 1 plus IGF 2 relative to IGFBP 3 as well as the increased concentrations of IGFBP 2 , which does not form a long lived ternary complex . ^^^ We have presently studied ternary complex formation between IGF , IGFBP 3 , and acid labile subunit ( ALS ) to further assess if IGF 1 bioavailability is increased in human fetal serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Comparison between insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) measurement in the diagnosis of growth hormone deficiency . ^^^ To analyze the utility of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) radioimmunoassay in the diagnosis of growth hormone deficiency ( GHD ) we measured IGF 1 and IGFBP 3 in sera from normal children ( n = 309 ) , short children ( n = 99 ) and patients with GHD ( n = 73 ) . ^^^ In 80 % and 93 % of classical GHD ( cGHD ) , IGF 1 and IGFBP 3 levels , respectively , were below the age related cutoff levels ( lower limit ) . ^^^ In 81 % and 88 % of normal short children ( NS ) , IGF 1 and IGFBP 3 levels , respectively , were above the age related cutoff levels . ^^^ Thus , both IGF 1 and IGFBP 3 were good parameters for screening GHD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The GH response to hypoglycaemia was impaired and plasma levels of both IGF 1 and the GH dependent IGF binding protein ( IGFBP 3 ) were low . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study was undertaken to investigate the effects of porcine IGFBP 3 on IGF 1 stimulated DNA synthesis in neonatal porcine skin fibroblasts . ^^^ Co incubation of IGFBP 3 with a maximally effective dose of IGF 1 ( 10 nM ) did not inhibit the stimulatory effects of IGF 1 on DNA synthesis . ^^^ In contrast , when IGFBP 3 at concentrations of 0 to 20 nM was co incubated with 1 nM IGF 1 , a bi phasic dose response was observed with IGFBP 3 being inhibitory only at a 10 to 20 fold molar excess to IGF 1 . ^^^ Based on the approximately equal molar ratio of IGFBP 3 : IGF 1 present in the circulation of control and pST treated pigs our results suggest that IGFBP 3 does not inhibit the mitogenic effects of IGF 1 . ^^^ In summary , these results indicate that the combination of IGFBP 3 with IGF 1 optimizes mitogenic signalling via the type 1 IGF receptor and suggest that IGFBP 3 does not inhibit the effects of ST that are mediated by IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , the regulation of follicle apoptosis by gonadotropins , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) was examined using a serum free culture of preovulatory follicles . ^^^ Cotreatment with IGFBP 3 and hCG dose dependently reversed the suppressive effect of hCG on apoptosis by 42 % , suggesting a mediatory role of endogenously produced IGF 1 . ^^^ The addition of IGFBP 3 also blocked the suppressive action of IGF 1 by 49 % , whereas it did not affect the suppressive action of an IGF 1 agonist or insulin . ^^^ Furthermore , IGFBP 3 did not affect IGF 1 action on steroid production . ^^^ To further substantiate the hypothesis that IGFBP 3 blocks the suppressive effect of hCG on apoptosis by neutralizing endogenously produced IGF 1 , solution hybridization analysis was performed , and hCG treatment was shown to increase IGF 1 messenger RNA levels in cultured follicles by 1 . 9 fold . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Primary preadipocyte cultures expressed IGF 1 , IGFBP 2 , and IGFBP 4 messenger RNAs ( mRNAs ) , and conditioned medium ( CM ) contained IGFBP 3 [ approximately 46 , 000 mol wt ( M ( r ) ) ] , IGFBP 4 ( 24 , 000 M ( r ) ) , and a 30 , 000 M ( r ) IGFBP identified by immunoblot as IGFBP 2 . ^^^ Like primary cultures , undifferentiated 3T3 L 1 cells expressed IGFBP 4 mRNA , but insignificant levels of IGF 1 and IGFBP 2 mRNAs . 3T3 L 1 cell CM contained IGFBP 3 and IGFBP 4 , and with the addition of IGF 1 , a 30 , 000 M ( r ) IGFBP was also present . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Spontaneous overnight and arginine stimulated GH secretion , insulin , IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 levels were measured before , during , and after daily 10 h sc infusions of saline or IGF 1 ( 20 micrograms / kg . h ) . ^^^ IGFBP 1 levels increased dramatically in diabetics and modestly in normal subjects in response to IGF 1 infusion , but IGFBP 3 levels were not significantly altered . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , measurements of early metabolic changes in GHD adults during the first 15 days after the start of GH replacement indicate that IGF 1 values reach maximal levels only after 8 12 days , that the measurements of changes in IGFBP 1 and IGFBP 3 levels probably do not contribute to a determination of the optimal GH replacement dose , that maximal N retaining effects occur within 2 5 days , after which adaptation occurs , that massive sodium retention occurs during this period , which spontaneously levels off , and that cumulative sodium retention closely correlates during this period with changes in BIA . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The patients had lower plasma insulin like growth factor 1 ( IGF 1 ) and serum IGF binding protein 3 ( IGFBP 3 ) concentrations than the controls . ^^^ Radiated subjects had decreased IGF 1 and IGFBP 3 concentrations compared to nonradiated subjects . ^^^ There was a positive correlation between nocturnal GH secretion , plasma IGF 1 , and serum IGFBP 3 levels , on the one hand , and pituitary height , on the other . ^^^ The finding of reduced IGF 1 levels in both radiated and nonradiated patients combined with decreased IGFBP 3 concentrations in radiated patients , indicates that cytotoxic chemotherapy may induce hepatic damage resulting in decreased IGF 1 synthesis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Sequential measurements were made of muscle strength ( one repetition maximum ) , body composition ( dual energy 10 ray absorptiometry ) , and circulating levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Low mol wt fragments of IGFBP 3 , which did not bind to IGF 1 or IGF 2 on Western ligand blot and did not cross link to IGF 2 , were demonstrated on Western immunoblot and were measurable by two different RIAs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with bST increased milk yield and concentrations of serum IGF 1 , hepatic IGF 1 mRNA , and serum IGF binding protein 3 , decreased concentration of serum IGF binding protein 2 , and did not alter abundance of mammary IGF 1 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recombinant human IGFBP 3 was cleaved by PSA , then incubated with 125I IGF 1 or 2 in the presence of varying concentrations of unlabelled peptides , and then cross linking electrophoresis and densitometric analysis were performed . ^^^ While the affinity of IGF 2 for the PSA generated IGFBP 3 fragments fell slightly compared to intact IGFBP 3 , the affinity of the PSA generated IGFBP 3 fragments for IGF 1 fell by ten fold . ^^^ When PSA was added to PC E cultures to which both IGF 1 or 2 and IGFBP 3 were added , the inhibitory effects of IGFBP 3 on IGF mitogenesis were reversed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , we examined the effects of 8 weeks of endurance training on changes in IGF 1 , IGF 1 binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and maximal aerobic power ( VO2max ) in 18 older individuals ( aged 66 . 1 + / 1 . 4 years , 10 men and eight women ) . ^^^ Although no mean group change in IGFBP 1 or IGFBP 3 was noted , the individual changes between IGF 1 and IGFBP 3 showed a tendency to be related in men ( r = . 48 , P = . 15 ) , but not in women ( . 21 , NS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 stimulated mainly IGF BP 3 production , but epidermal growth factor ( EGF ) and transforming growth factor alpha ( TGF alpha ) stimulated predominantly IGF BP 4 secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) is the principal carrier protein of IGF 1 in the circulation . ^^^ Regardless of the exact mechanism of action of IGF 1 on ovarian steroidogenesis , alterations in the level of IGFBP 3 may play a significant role in regulating the concentration of IGF 1 in hyperandrogenism . ^^^ We have postulated that androgens reduce the circulating IGFBP 3 concentration through a mechanism similar to its suppression of the hepatic production of sex hormone binding globulin ( SHBG ) , thereby increasing bioavailable IGF 1 and amplifying its impact on ovarian steroidogenesis . ^^^ Serum was assayed for total T , GH , and SHBG , and plasma was assessed for IGF 1 , insulin ( INS ) , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors 1 and 2 ( IGF 1 and 2 ) are present in serum primarily within a ternary complex consisting of IGF , IGF binding protein 3 ( IGF 3 ) and acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of IGF 1 are modulated by several IGF binding proteins including IGFBP 3 , the main circulating carrier of IGF 1 , and IGFBP 4 , the main IGF binding protein produced by vascular smooth muscle cells in vitro . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) has been shown to be elevated in ESRD , possibly resulting in inhibition of the bioactivity of IGF 1 . ^^^ Potentially , the removal of IGFBP 3 may improve the bioactivity of IGF 1 , with resulting improvement in growth . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) has been shown to be elevated in ESRD , possibly resulting in inhibition of the bioactivity of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both doses of pST increased ( P < . 01 ) serum and follicular fluid ( FFL ) concentrations of IGF 1 and activity of IGF binding protein ( IGFBP ) 3 and 36 kDa IGFBP in all three lines ; IGFBP 3 was the predominant IGFBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The formation in serum of binary complexes between IGF 1 or 2 and either of six IGF binding proteins ( IGFBP 1 to 6 ) and , in particular , of long lived ternary complexes between IGF 1 or 2 , IGFBP 3 , and acid labile subunit is thought to regulate IGF 1 bioavailability by increasing its serum half life . ^^^ The present study assesses the bioavailability of circulating IGF 1 in 19 to 35 wk gestation human fetuses in utero 1 ) by quantitative RIA measurements of IGF and IGFBP in serum and 2 ) by examining whether serum proteolysis of IGFBP 3 may further increase IGF 1 bioavailability . ^^^ Fetal serum concentrations of IGFBP 3 , IGF 1 , and IGF 2 were low with marked or only modest increases with gestational age ( p < 0 . 001 , p < 0 . 005 , and p < 0 . 05 , respectively ) . ^^^ The mean molar ratio between IGF 1 plus 2 and IGFBP 3 demonstrated a molar excess of IGF ( 50 % ) similar to that in adolescents but in contrast to the 1 : 1 molar ratio in adults . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) , the carboxyterminal propeptide of type 1 collagen ( PICP ) , the carboxyterminal pyridinoline crosslinked telopeptide of type 1 collagen ( ICTP ) , and the aminoterminal propeptide of type 3 procollagen ( PIIINP ) were studied in 10 prepubertal children with asthma ( mean age 9 . 0 years ) . ^^^ No statistically significant effects on serum concentrations of IGF 1 and IGFBP 3 were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Mutant S111A / S113A , despite being non phosphorylated and non glycosylated , is functionally similar to the wild type IGFBP 3 in terms of IGF 1 binding . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes in insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , growth hormone ( GH ) binding protein , erythrocyte IGF 1 receptors , and growth rate during GH treatment . ^^^ To assess the relative determinants of growth rate , we measured serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and GH binding protein ( GHBP ) as well as IGF 1 erythrocyte receptor specific binding ( SB ) in 14 prepubertal GH deficient children before and during the first year of treatment with 0 . 043 mg / kg . day GH . ^^^ Serum IGF 1 and IGFBP 3 levels , measured by RIA , were significantly increased by 2 weeks and showed progressive increases throughout the year of GH therapy . ^^^ Growth rate ( height velocity SD score adjusted for bone age ) correlated best with the 12 month changes in IGFBP 3 ( r = 0 . 81 ; P < 0 . 001 ) and IGF 1 ( r = 0 . 72 ; P = 0 . 005 ) , and to a lesser extent with the 12 month absolute IGFBP 3 ( r = 0 . 58 ; P = 0 . 04 ) and the 6 month change in IGFBP 3 ( r = 0 . 55 ; P = 0 . 05 ) . ^^^ IGF 1 and IGFBP 3 show progressive increases , whereas the erythrocyte IGF 1 receptor binding capacity decreases by 6 months , and GHBP shows little change during the first year of GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 and IGF 2 were measured by RIA , and IGFBP 3 was measured by both RIA and immunoradiometric assay . ^^^ In the growth study , circulating maternal IGF 1 and IGFBP 3 levels were not different in the three groups , but fetal IGF 1 and IGFBP 3 levels were significantly lower in the UPI fetuses ( IGF 1 , 6 . 9 + / 0 . 5 micrograms / L ; IGFBP 3 , 547 + / 70 micrograms / L ) than in either the nonstarved SGA fetuses ( IGF 1 , 27 . 8 + / 6 . 3 ; IGFBP 3 , 769 + / 41 micrograms / L ; P < 0 . 01 ) or the AGA fetuses ( IGF 1 , 39 . 4 + / 3 . 4 ; IGFBP 3 , 872 + / 91 micrograms / L ; P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since the liver is the major source of IGF and IGFBP production , we have therefore examined whether levels of IGFs ( IGF 1 and IGF 11 ) and IGFBPs ( IGFBP 1 and IGFBP 3 ) differ when insulin is infused into the portal or peripheral vascular system . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that the 150 kDa fraction of adult rat serum includes 1 ) ternary complexes of intact IGFBP 3 ( which binds IGF 1 and IGF 2 with similar affinity ) , endogenous IGF 1 , and the ALS ; and 2 ) binary complexes of proteolytically nicked IGFBP 3 and ALS that bind IGF 2 preferentially . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Of interest , the level of IGF 1 protein in these samples was also markedly reduced following severe burn injury similar to IGFBP 3 . ^^^ The results of this study suggest that a marked reduction of serum IGF 1 seen in burn patients is associated with a significant reduction of IGFBP 3 , a major IGF 1 binding protein in human serum . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with human IGFBP 3 and oIGFBP 3 , the two variants of oIGFBP 4 exhibited lower relative binding to amino terminally modified IGF 1 analogues in a competitive IGF binding assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Production of insulin like growth factor binding protein ( IGFBP ) , IGFBP 3 protease , and expression of IGF 1 receptors by cells of the sheep immune system . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the responses of these cells to exogenously added IGF 1 , IGF 2 , or IGFBP 3 . ^^^ When IGFBP 3 was administered together with IGF 1 or IGF 2 , it further increased the mitogenic response observed in PC 3 ARCA cells , but no effect on PC 3 Neo cells was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 induced by CSF 1 was biologically active because a natural ligand of IGF 1 , IGF binding protein 3 , caused significant down regulation of cellular proliferation , and this could be reversed by the addition of exogenous IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased IGFBP 3 and 6 production in response to RA + forskolin was accompanied by a decrease in IGF stimulated thymidine incorporation into DNA ; by contrast , the bioactivity of an IGF analog that does not bind with IGFBPs , [ Gln 3 , Ala 4 , Tyr 15 , Leu 16 ] IGF 1 , was unchanged under these conditions . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 , the major circulating IGFBP , is unique in combining with a glycoprotein , the acid labile subunit ( ALS ) , to form a ternary complex with IGF 1 or IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We aimed to assess the direct effects of liver dysfunction on serum levels of IGF 1 and its major binding proteins by measuring fasting levels of growth hormone , IGF 1 , IGFBP 1 , IGFBP 3 , insulin , C peptide , and glucose in 35 patients with cirrhosis and during an oral glucose tolerance test in 16 of those patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , basal levels of serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , IGF binding protein 3 ( IGFBP 3 ) , insulin , pro insulin , and blood glucose were measured during habitual energy intake as well as during the hypocaloric diet . ^^^ No differences between obese subjects and normal controls were present regarding IGF 1 , IGFBP 3 , or IGF I / IGFBP 3 molar ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After rhIGF 1 treatment , plasma IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 2 all increased significantly , whereas IGFBP 3 did not change . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) is a GH dependent protein that binds insulin like growth factor 1 ( IGF 1 ) in the circulation and modulates its action at the tissue level . ^^^ IGFBP 3 levels were compared to IGF 1 and random GH levels before and after transsphenoidal surgery . ^^^ Concordance among IGFBP 3 , IGF 1 , and GH suppressibility by glucose was also determined . ^^^ IGFBP 3 and IGF 1 levels were correlated in patients with untreated acromegaly ( r = 0 . 650 ; P = 0 . 0162 ) and after transsphenoidal surgery ( r = 0 . 644 ; P = 0 . 0001 ) . ^^^ Neither IGF 1 nor IGFBP 3 correlated with random GH levels before surgery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Jejunal mRNA content for IGF 1 , IGF 1 receptor , and IGF binding proteins 3 and 4 ( IGFBP 3 , 4 ) were determined by Northern blotting . ^^^ IGF 1 treatment significantly increased IGFBP 3 tissue mRNA levels in both transected and transplanted animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
While recombinant human IGF binding protein ( IGFBP 3 ) had no effect by itself of basal [ 3H ] thymidine incorporation , it inhibited the effect of IGF 1 in a dose dependent manner ; however , it had no effect on the stimulation by ( QAYL ) IGF F . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
While insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were correlated directly to log GH ( r = 0 . 77 and r = 0 . 66 , p < 0 . 001 ) , an inverse and weaker relationship was evident between GHBP measured by RIA and IGF 1 or IGFBP 3 ( r = 0 . 61 and r = 0 . 57 , p < 0 . 01 ) . ^^^ In contrast , no correlation could be detected between data of the functional GHBP assay and IGF 1 or IGFBP 3 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURES : Fasting insulin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , T , androstenedione ( A ) , and LH . ^^^ Pretreatment IGF 1 and IGFBP 3 levels were similar in PCOS and controls . ^^^ CONCLUSIONS : These data suggest that women with PCOS may be more sensitive to the effects of octreotide in decreasing IGF 1 and increasing IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURES : Seminal plasma and serum levels of IGF 1 , IGFBP 1 , and IGFBP 3 were determined by commercially available assays , furthermore , samples were subjected to Western ligand blotting . ^^^ CONCLUSIONS : A considerable amount of seminal plasma IGF 1 and IGFBP 3 may be of testicular origin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes induced by non enzymatic glycosylation of IGF binding protein 3 : effects on its binding properties and on its modulatory effect on IGF 1 mitogenic action . ^^^ The aim of this study was to demonstrate the feasibility of in vitro non enzymatic glycosylation of IGF binding protein 3 ( IGFBP 3 ) and whether this process affects its binding properties and its modulatory effect on IGF 1 mitogenic activity . ^^^ Glycated IGFBP 3 bound larger amounts of 125I labelled IGF 1 than its non glycated form . ^^^ Preincubation of fibroblasts with CM containing IGFBP 3 for 16 h before the addition of IGF 1 enhanced the stimulatory effect of the hormone on thymidine incorporation into cell DNA . ^^^ These results provide further evidence of the in vitro glycation of IGFBP 3 and demonstrate that this process affects both its binding properties and its enhancing effect on IGF 1 mitogenic activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The processing of insulin like growth factor 1 ( IGF 1 ) by a cultured kidney cell line is altered by IGF binding protein 3 . ^^^ When OK cells were exposed to [ 125I ] IGF 1 in the presence of IGF binding protein 3 ( IGFBP 3 ) cell association ( binding and internalization ) was reduced significantly . ^^^ This study also shows that IGFBP 3 inhibits the binding and uptake of [ 125I ] IGF 1 by these kidney cells . ^^^ However , once IGF 1 is internalized , IGFBP 3 enhances degradation . ^^^ Although the mechanism of this paradoxical action requires further study , analysis of the products of degradation suggests that the same enzymes are involved in IGF 1 degradation regardless of whether IGFBP 3 is present . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In bovine fibroblasts , IGF 1 treatment induced IGFBP 3 and IGFBP 5 mRNA and protein secretion , and had a moderate effect on IGFBP 4 expression . ^^^ In human fibroblasts , IGF 1 treatment increased levels of IGFBP 3 and decreased levels of IGFBP 4 without influencing mRNA expression . ^^^ Dexamethasone alone decreased IGFBP 3 , IGFBP 4 , and IGFBP 5 mRNA , but it had no significant effect on IGFBP 3 , 4 , or 5 expression in the presence of IGF 1 . ^^^ Bovine fibroblasts may provide a useful model system to probe the molecular mechanisms of IGFBP 3 , 4 , and 5 gene expression and regulation by IGF 1 and glucocorticoid , whereas human fibroblasts may be suitable for studying posttranscriptional interactions . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In nephrotic rat serum , binding to IGFBP 3 is reduced and Western immune analysis demonstrates an approximately 27K fragment that does not bind IGF 1 , suggesting in vivo proteolysis of IGFBP 3 . ^^^ Because the 150K ternary complex in serum but not the smaller binding protein complex is restricted to the intravascular space , the shift of binding from IGFBP 3 ( ternary complex ) to IGFBP 2 ( binary complex ) in nephrotic rat serum may help to maintain tissue availability despite the reduction in serum IGF 1 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the serum concentrations of IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and testosterone in relation to blood Hb in 60 healthy prepubertal or early pubertal boys twice , with a 9 mo interval . ^^^ Serum IGF 1 and testosterone levels were measured by RIA , and serum IGFBP 3 was measured by monoclonal immunofluorometric assay . ^^^ Positive correlations were observed between the concentrations of blood Hb and serum IGF 1 at the first examination ( r = 0 . 36 , p = 0 . 008 ) and Hb and IGFBP 3 at both examinations ( r = 0 . 53 , p < 0 . 001 , and r = 0 . 39 , p = 0 . 003 ) . ^^^ Our results show that blood Hb is positively correlated to serum IGF 1 and IGFBP 3 levels , indicating indirectly the involvement of growth hormone in the regulation of physiologic Hb concentration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The purposes of this study were to explore whether serum levels of insulin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) and IGFBP 3 in both maternal and fetal compartments were affected by the stress of labor , and to investigate the relationship between the fetal birthweight and serum levels of insulin , IGF 1 and IGFBPs . ^^^ Serum concentrations of insulin , IGF 1 , IGFBP 1 and IGFBP 3 were determined by radioimmunoassays ( insulin , IGFBP 1 and IGFBP 3 ) and immunoradiometric assay ( IGF 1 ) . ^^^ As for all measurements ( insulin , IGF 1 , IGFBP 1 and IGFBP 3 ) , serum levels in pregnant women ( from both NSD and CS ) were strikingly higher than those in the fetus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 , and IGFBP 4 increased under r hGH and , in contrast to GH BP activity , remained high throughout the study . ^^^ In conclusion , in girls with Turner ' s syndrome , GH BP activity , IGF 1 , IGFBP 3 , and IGFBP 4 are induced by r hGH . ^^^ However , the increase of IGF 1 and IGFBP 3 does not require an increased level of the cellular growth hormone receptors , as suggested by the unchanged +360 d values of plasma GH BP activity compared with baseline . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 were measured at regular intervals throughout the study . ^^^ RESULTS : Serum IGF 1 and IGFBP 3 levels were significantly lower while serum IGFBP 1 levels were significantly higher in the diabetic subjects . ^^^ Pirenzepine had no effect on serum IGF 1 , IGFBP 1 or IGFBP 3 in diabetic subjects but caused a significant increase in serum IGF 1 and IGFBP 3 levels in normal subjects . ^^^ Pyridostigmine had no effect on IGF 1 , IGFBP 1 or IGFBP 3 in either diabetic or normal subjects . ^^^ IGFBP 1 levels were significantly correlated with fasting plasma glucose but no correlation was demonstrated between measures of diabetic control and serum IGF 1 or IGFBP 3 levels in diabetic subjects , nor was there any correlation between GH responses to GHRH alone or after pirenzepine or pyridostigmine pretreatment and serum levels of IGF 1 , IGFBP 1 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : The circulating concentrations of GH , IGF 1 , IGF 2 and IGFBP 3 were measured in acromegalic patients before and after 3 , 6 , 9 , and 12 months of treatment with octreotide ( group 1 : n = 5 ) , and compared with those found in a group of patients ( group 2 ) treated with bromocriptine ( n = 3 ) , cabergoline ( n = 7 ) radiotherapy ( n = 3 ) or surgical therapy ( n = 2 ) . ^^^ In pools of serum obtained from patients treated with octreotide , dopaminergic drugs , surgery and radiation , before and after therapy , immunoreactive IGF 1 and IGFBP 3 were also evaluated after Superdex 200 gel filtration in neutral conditions . ^^^ RESULTS : Before treatment , the concentration of IGF 1 and IGFBP 3 were above the normal range in all patients , while IGF 2 levels were slightly reduced . ^^^ After treatment with octreotide , IGF 1 ( P = 0 . 004 ) , IGF 2 ( P = 0 . 02 ) and IGFBP 3 ( P < 0 . 001 ) were significantly reduced as compared to basal levels . ^^^ After gel filtration immunoreactive IGF 1 and IGFBP 3 were found in the 150 kDa mol . wt . region in serum obtained from untreated patients and from treated patients of group 2 , while in the serum of octreotide treated patients the IGF 1 and IGFBP 3 peaks were shifted to the 60 kDa mol . wt . region , thus suggesting that the acid labile subunit of the 150 kDa complex was drastically reduced . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 1 is antagonistic to the insulin like and growth promoting effects of IGF 1 , and IGFBP 3 holds IGFs in the circulation by associating with IGFs and an acid labile subunit to form a ternary complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Previous studies have shown that FSH lowers the abundance of IGFBP 3 protein in Sertoli cell culture medium , suggesting that this binding protein has a physiological role in modulating insulin like growth factor 1 ( IGF 1 ) activity in the testis . ^^^ We then examined whether or not IGFBP 3 could alter the effects of IGF 1 on cultured Sertoli cells . ^^^ To determine whether or not IGFBP 3 modulates IGF 1 activity in the Sertoli cell , recombinant human non glycosylated rIGFBP 3 was added to Sertoli cell cultures in the presence or absence of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined excretion of urinary insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) and their major binding protein IGFBP 3 in comparison to their respective serum concentration in nine healthy female volunteers ( median age 25 years , range 22 27 ) under baseline conditions and after stimulation with recombinant human growth hormone ( rhGH ) , 4 . 5 IU twice daily subcutaneously for a period of 3 days . ^^^ The mean ( + / SD ) urinary concentrations of IGF 1 ( 0 . 08 + / 0 . 07 micrograms / l ) , IGF 2 ( 1 . 02 + / 0 . 47 micrograms / l ) and IGFBP 3 ( 19 . 1 + / 6 . 9 micrograms / l ) were two to three orders of magnitude lower than in serum . ^^^ The ratio of IGF 2 over IGF 1 concentration in urine ( 13 : 1 ) was five times higher than in serum ( 2 . 5 : 1 ) , and the ratio of IGFBP 3 over the sum of IGF 1 and IGF 2 in urine ( 17 : 1 ) was four times higher than in serum ( 4 : 1 ) . ^^^ Urinary excretion was 63 . 3 + / 46 . 6 ng . m 2 . 24h 1 for IGF 1 , 1002 + / 598 ng . m 2 . 24h 1 for IGF 2 and 18039 + / 4983 ng . m 2 . 24h 1 for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The liver is thought to be the major source of circulating insulin like growth factor ( IGF 1 ) and IGF binding protein 1 ( IGFBP 1 ) , whereas the primary production site of circulating IGFBP 3 remains unknown . ^^^ As other tissues may contribute to the circulating pool of IGF 1 and IGFBP , the aim of the present study was to assess the hepatic and renal arterio venous difference and production rates of IGF 1 , IGFBP 1 , IGFBP 3 , and GH in cirrhotic patients ( n = 22 ) and matched control subjects ( n = 27 ) . ^^^ No significant differences were found in the values obtained simultaneously from hepatic , renal , and brachial veins or femoral artery , which suggests that no major net production or release of IGFBP 3 or IGF 1 occurs in these tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the treated diabetics IGF 1 and IGF 2 were reduced ; IGFBP 2 ( only in prepubertal subjects ) and IGFBP 3 were increased . ^^^ Furthermore , age adjusted values of IGF 1 , IGF 2 , and IGFBP 3 were lower in prepubertal than in pubertal patients . ^^^ Strong correlations were observed between height SDS and IGF 1 SDS , IGF 2 SDS , and IGFBP 3 SDS in prepubertal subjects who had had IDDM for at least 2 yr , but not in adolescents . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before insulin therapy , serum IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , and GH binding protein ( GHBP ) levels were significantly decreased , whereas IGFBP 1 and cortisol were significantly increased in diabetic children compared to those in an age , sex , and stage of puberty matched control group . ^^^ Improvement in glycemic control , as determined by a change in hemoglobin A1c , correlated positively with improvement in IGF 1 , IGF 2 , IGFBP 3 , GHBP , and weight gain after 1 month of insulin therapy . ^^^ The decreases in IGF 1 , IGF 2 , and IGFBP 3 may in part be due to a decrease in the GHBP / receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As demonstrated by gel permeation chromatography on fast performance liquid chromatography , incubation for 2 h at 20 C of this preparation with [ 125I ] IGF 1 and recombinant IGFBP 3 ( rIGFBP 3 ) allows the reconstitution of a complex of about 150 kilodaltons . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , we 1 ) determined whether the impaired spontaneous 24 h GH secretion as well as the blunted GH response to provocative testing in obese subjects are persistent disorders or transient defects reversed with weight loss and 2 ) investigated 24 h urinary GH excretion and basal levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , as well as insulin in obese subjects before and after a massive weight loss . ^^^ In the obese subjects , 24 h spontaneous GH release profiles and the GH responses to insulin induced hypoglycemia and L arginine as well as basal IGF 1 levels and the IGF I / IGFBP 3 molar ratio were decreased , whereas insulin levels were elevated compared to those in normal subjects . ^^^ The decreased 24 h spontaneous GH release profiles , the decreased GH responses to insulin induced hypoglycemia and L arginine , the decreased basal IGF 1 levels and IGF I / IGFBP 3 molar ratio , as well as the elevated insulin levels were returned to normal after a massive weight loss in the obese subjects . ^^^ In conclusion , the present study has shown reversible defects in 24 h spontaneous GH release profiles , basal IGF 1 levels , and the IGF I / IGFBP 3 molar ratio in obese subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a double blind , placebo controlled trial , the effects of recombinant human growth hormone were studied on cerebrospinal fluid concentrations of growth hormone , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , monoamine metabolites , neuropeptides and endogenous opioid peptides . ^^^ The cerebrospinal fluid IGF 1 concentration increased from 0 . 67 + / 0 . 04 to 0 . 99 + / 0 . 10 micrograms / l ( p = 0 . 005 ) and the IGFBP 3 concentration rose from 13 . 4 + / 1 . 25 to 17 . 5 + / 1 . 83 micrograms / l ( p = 0 . 002 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During refeeding , jejunal IGF binding protein 3 ( IGFBP 3 ) mRNA abundance was reduced relative to that of IGF 1 , possibly amplifying enterotrophic actions of IGF 1 . ^^^ Localization of IGFBP 3 to subepithelial cells in lamina propria of jejunum indicates that IGFBP 3 derived from lamina propria may modulate IGF 1 action on adjacent epithelium . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood , collected from a cohort ( n = 3 ) of these patients within 24 h of their discharge from the hospital , indicated that IGF 1 and IGF 2 were still reduced ( 30 % ) and that the decrease in IGFBP 3 and the elevation in IGFBP 1 were still evident at this time . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To study the role of insulin like growth factor ( IGF ) molecules in IPF we evaluated spontaneous release of IGF 1 and IGFBP 3 in bronchoalveolar lavage cells from control subjects and from patients with IPF . ^^^ These data are compatible with the hypothesis that IGFBP 3 increases the bioactivity of IGF 1 derived from a variety of lung tissues contributing to the fibrosis and remodeling seen in IPF . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We hypothesized that levels of both hormones , their dominant BPs and liver IGF 1 mRNA would fall , and that part of the mechanism for decreasing serum IGF 1 would be enhanced IGFBP 3 protease activity . ^^^ By day 30 , caloric deprivation to 40 % lowered serum GH , GHBP , IGF 1 and IGFBP 3 , and liver IGF 1 mRNA . ^^^ Protein deprivation lowered serum GH , IGF 1 and IGFBP 3 , and liver IGF 1 mRNA , while GHBP levels were normal . ^^^ The reduced total IGF 1 under these dietary conditions could not be explained by an increase in IGFBP 3 protease activity , or a decrease in the association of IGF 1 with IGFBP 3 and the acid labile subunit . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In normal term FCS , the following levels ( + / SE ) were present : IGF 1 , 76 + / 16 ; IGF 2 , 401 + / 38 ; IGFBP 3 , 700 + / 112 ; IGFBP 1 , 77 + / 10 ng / mL ; and insulin , 3 . 8 + / 1 . 6 microU / mL . ^^^ In IUGR FCS , IGF 1 , IGF 2 , and IGFBP 3 were significantly reduced , and IGFBP 1 was 7 fold higher than in FCS from normal weight fetuses . ^^^ In LGA FCS , IGF 1 , insulin , and IGFBP 3 were significantly increased , whereas IGFBP 1 was significantly decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Administration of r hGH evoked a significant increase in plasma concentrations of GH , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) levels became significantly higher in the GH group ( p = 0 . 001 and p = 0 . 014 , respectively ) . ^^^ After two weeks , IGF 1 and IGFBP 3 levels stabilized . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of this investigation was to study the effect of relatively high dose IGF 1 therapy given for several months , on serum levels of IGF 1 , IGF 2 and IGFBP 3 , and on IGF 1 pharmacokinetics in patients with growth hormone insensitivity due to GH receptor dysfunction . ^^^ MEASUREMENTS : Serum was sampled at baseline and at 3 8 months , for determination of IGF 1 , IGF 2 and IGFBP 3 by radioimmunoassay , and for evaluation of IGFBPs and IGFBP 3 protease activity by Western ligand blot and protease assay , respectively . ^^^ Serum IGFBP 3 levels failed to rise with prolonged IGF 1 treatment . ^^^ CONCLUSIONS : IGF 1 administration does not increase serum levels of IGFBP 3 or significantly alter IGF 1 pharmacokinetics . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the cyclic changes of serum insulin like growth factor ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 levels during ovulatory menstrual cycle . ^^^ MAIN OUTCOME MEASURES : Serum concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 from women with regular menstrual cycles were assayed . ^^^ By contrast , there were no cyclic changes of serum IGF 1 and IGFBP 3 levels in women with regular menstrual cycles , including both normal nad inadequate luteal functions . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have examined the expression of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , and IGF binding protein 3 ( IGFBP 3 ) in the rat distal nephron during increased cell work load and hypertrophy , induced by the diuretic , furosemide . ^^^ Whereas transcripts of IGF 1 and IGFBP 3 mRNA showed little or no increase in extracts from furosemide treated kidney cortices , IGFBP 1 mRNA was increased threefold 18 hours after furosemide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since previous studies suggested that IGFBPs can inhibit DNA synthesis stimulated by IGF 1 , we wondered whether the mitogenic effect of bFGF was altered by exogenous IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All groups experienced a significant increase in serum IGF 1 and IGFBP 3 levels over the treatment period , accompanied by significant decreases in IGF 2 ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have asked whether the IGFBP 3 has an effect on cell growth , which is independent of its ability to bind IGF 1 and thus inhibit the activation of the IGF 1 receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have studied fetal growth and serum levels of GH , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) during pregnancy , as well as birth weight and hormone levels after delivery in a 25 year old woman with idiopathic , isolated GH deficiency diagnosed at the age of 7 years . ^^^ Serum levels of GH , IGF 1 and IGFBP 3 were measured monthly during pregnancy after 3 days off GH therapy . ^^^ Serum GH and IGF 1 levels increased during the second half of pregnancy ; serum IGFBP 3 remained constant throughout pregnancy at a normal level . ^^^ Serum levels of GH fell within 1 h after delivery , and levels of IGF 1 and IGFBP 3 decreased into the range of GH deficient women 4 days after . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of growth hormone dependent insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) , the carboxy terminal propeptide of type 1 procollagen ( PICP ) , the carboxy terminal pyridinoline cross linked telopeptide of type 1 collagen ( ICTP ) and the amino terminal propeptide of type 3 procollagen ( PIIINP ) were studied in 14 prepubertal children with asthma ( mean age 9 . 7 years ) during treatment with inhaled budesonide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were determined by radioimmunoassay in term infants ( n = 51 ) at 0 , 2 , 4 , 6 , and 12 mo and preterm infants ( n = 51 ) at 3 ( 28 . 4 wk postconceptional age ) , 2 , 1 . 5 , 1 , 0 , 2 , 4 , 6 , 9 , and 12 mo from expected term . ^^^ In preterm infants , IGF 1 increased between 3 and 2 mo from 0 . 75 to 10 . 4 nM , decreased between 2 and 9 mo to 7 . 3 nM , and increased again between 9 and 12 mo to 10 . 1 nM ( p < 0 . 0001 ) , whereas IGFBP 3 increased relatively little from 3 to 0 mo ( 14 . 2 to 30 . 2 nM , p < 0 . 05 ) and plateaued from 2 to 12 mo ( 49 . 8 to 62 . 3 nM ) . ^^^ At 0 mo , IGF 1 and IGFBP 3 were the same in term and preterm infants , but preterm infants had higher IGF 1 from 2 through 12 mo ( p < 0 . 05 ) , higher IGFBP 3 at 2 and 4 mo ( p < 0 . 05 ) , and lower IGFBP 3 at 12 mo ( p < 0 . 05 ) . ^^^ IGF 1 and IGFBP 3 were correlated at most ages . ^^^ First year IGF 1 and IGFBP 3 are influenced by study age and by gestational age , even after adjusting for early birth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the IGF 1 stimulated increase in [ 3H ] AIB uptake was completely prevented by concurrent treatment with IGFBP 3 . ^^^ Preincubation of MDGp 1 cells with IGFBP 3 dose dependently inhibited FSH and IGF 1 stimulated P 4 secretion . ^^^ Recombinant human insulin like growth factor binding protein 3 ( rhIGFBP 3 ) effects on basal , insulin like growth factor 1 ( IGF 1 ) , and follicle stimulating hormone ( FSH ) stimulated progesterone ( P 4 ) secretion and [ 3H ] aminoisobutyric acid ( AIB ) uptake by primary porcine granulosa cells ( MDGs ) and MDGs that have been passaged once ( MDGp 1 ) were assessed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 and IGFBP 6 were used to study whether both IGF 1 and IGF 2 play a role in auto / paracrine stimulation of rat osteoblast growth . ^^^ Consistent with their IGF binding affinities , IGFBP 3 blocked both IGF 1 and IGF 2 stimulated DNA synthesis , whereas IGFBP 6 preferentially blocked IGF 2 stimulated DNA synthesis . ^^^ Because IGFBP 6 preferentially binds IGF 2 and is much less potent than IGFBP 3 in decreasing basal DNA synthesis in calvaria cells , IGF 1 but not IGF 2 appears to be an important auto / paracrine stimulator of DNA synthesis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 was in the normal range and increased significantly during GH treatment , although relatively less than IGF 1 , such that the molar ratio between IGF 1 and IGFBP 3 increased with GH treatment , whereas the ratio between IGF 1 + IGF 2 and IGFBP 3 remained unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In circulation , most of IGF 1 is bound to IGF binding protein 3 ( IGFBP 3 ) . ^^^ To assess the availability of IGF 1 for receptor binding , we determined serum levels of IGF 1 and IGFBP 3 and IGF I / IGFBP 3 ratios . ^^^ Premenopausal cases showed elevated IGF 1 serum concentrations , decreased IGFBP 3 levels and increased IGF I / IGFBP 3 ratios . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that continuous sc infusion of GH induced serum IGF 1 and IGFBP 3 levels more effectively than daily sc injections . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) in healthy infants , children , and adolescents : the relation to IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , age , sex , body mass index , and pubertal maturation . ^^^ Circulating IGF 1 and 2 are bound to specific insulin like growth factor ( IGF ) binding proteins ( IGFBPs ) , of which IGFBP 3 binds the majority of the IGFs . ^^^ IGFBP 3 levels are regulated by GH and have been suggested to provide additional information on GH secretory capacity compared to IGF 1 . ^^^ Therefore , we measured IGFBP 3 , IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 2 levels by RIA in 907 healthy children to establish well characterized normative data on IGFBP 3 according to age , sex , and pubertal stage and to study the complex relationship between IGFs and their BPs in puberty . ^^^ Age , sex , height , BMI , and pubertal maturation were all important factors in determining the circulating levels of IGFBP 3 , whereas IGF 1 levels were unaffected by BMI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 is positively correlated with fetal weight ( r = 0 . 66 , P = 0 . 03 ) , circulating IGFBP 3 ( r = 0 . 54 , P = 0 . 01 ) and IGFBP 4 ( r = 0 . 52 , P = 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lactating dams ( day 14 postpartum ) were separated from their pups and injected intraperitoneally with 0 . 45 microCi 125I IGF 1 or 125I IGFBP 3 . ^^^ Sephacryl S 200 gel filtration chromatography demonstrated that the 125I IGF 1 in both serum and milk was primarily in the 150 kDa IGF binding complex , whereas the distribution of 125I IGFBP 3 differed between serum and milk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We tested the sensitivity and specificity of the IGFBP 3 assessment in comparison with the measurement of IGF 1 . ^^^ DESIGN : We assessed the IGFBP 3 and IGF 1 circulating levels in normal subjects and patients with GHD or idiopathic short stature ( ISS ) . ^^^ IGFBP 3 and IGF 1 circulating levels were measured by radioimmunoassay . ^^^ RESULTS : IGFBP 3 and IGF 1 levels were closely related ( r = 0 . 51 , P < 0 . 0001 ) and IGFBP 3 was less age dependent than IGF 1 ( r = 0 . 57 , P < 0 . 02 vs r = 0 . 64 , P = 0 . 0001 ) . ^^^ Sensitivity ( true positive ratio ) and specificity ( true negative ratio ) of IGFBP 3 measurement were 50 and 92 % respectively , whereas sensitivity and specificity of IGF 1 assessment were 75 and 90 % respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In order to determine whether this excess IGFBP 3 accumulation plays a role in the observed attenuation of DNA synthesis in senescent HDF , we examined the response of these cells to IGF 1 and two IGF 1 functional analogs , [ QAYL ] IGF 1 and insulin , both of which have extremely low binding affinity for IGFBP 3 but which exert their mitogenic effect via the IGF 1 plasma membrane receptor . ^^^ Addition of recombinant IGFBP 3 to young cells at a constant molar ratio of 1 : 1 with respect to IGF 1 significantly attenuated the response to IGF 1 and abolished the response at a 2 : 1 molar ratio . ^^^ Overexpression of insulin like growth factor binding protein 3 by senescent human fibroblasts : attenuation of the mitogenic response to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) increased significantly after the start of IGF 1 treatment , compared with mean postoperative levels . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) increased significantly after the start of IGF 1 treatment , compared with mean postoperative levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IL 1 beta in concentrations of 0 . 1 , 1 , and 10 ng / ml caused a 1 . 5 , 4 , and 6 . 5 fold induction of IGFBP 3 expression , respectively , whereas IGF 1 mRNA levels were decreased in a dose dependent manner . ^^^ In conclusion , IL 1 beta inhibits IGF 1 but increases IGFBP 3 expression in Leydig cells , and this may contribute to the inhibitory effects of IL 1 beta on Leydig cell steroidogenesis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lastly , IGFBP 2 and IGFBP 3 are mitogenic for UMR 106 . 01 rat osteosarcoma cells , inducing an increase in cell number to 125 % and 142 % of control cell counts after 48 h of incubation with 1000 ng / ml IGFBP 2 and 3 , whereas IGF 1 , IGF 2 and Long R 3 IGF 1 did not stimulate proliferation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the early catabolic flow phase of severe injury , when plasma levels of GH and IGF 1 are low versus uninjured levels , the role of IGFBP 3 has not been investigated . ^^^ In the catabolic flow phase of injury , plasma levels of GH , IGF 1 , and IGFBP 3 were significantly reduced by 50 % , 46 % , and 45 % , respectively . ^^^ There was a significant linear inverse relationship between IGFBP 3 and age and also a positive correlation between IGFBP 3 and IGF 1 in both control and injured subjects . ^^^ The ratio of IGFBP 3 to IGF 1 was not changed in trauma victims . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes in serum levels of IGF 1 and IGFBP 3 in children with chronic renal failure . ^^^ The significance of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels in uremia is still controversial . ^^^ In this study we measured serum IGF 1 by immunoradiometric assay ( IRMA ) and IGFBP 3 levels by specific radioimmunoassay ( RIA ) in 28 children ( aged 3 16 years ) with end stage ( n = 14 , on hemodialysis ) and pre terminal renal failure ( n = 14 ) and in 15 age matched healthy children . ^^^ Neither IGF 1 nor IGFBP 3 levels significantly correlated with mean height SDS for bone age or for chronological age in either non dialysis patients or pubertal ( n = 10 ) or prepubertal patients ( n = 18 ) . ^^^ These data was consistent with the concept that growth in CRF was not related to abnormalities in serum IGF 1 or IGFBP 3 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of patients with Ullrich Turner syndrome with conventional doses of growth hormone and the combination with testosterone or oxandrolone : effect on growth , IGF 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 94 children with chronic renal failure ( CRF ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The metabolic half life of circulating IGF 1 is prolonged by its binding to IGFBP 3 . ^^^ Adjuvant rhGH significantly increases plasma levels of GH , IGF 1 , IGFBP 3 , and insulin . ^^^ The bioavailability of IGF 1 is increased , as indicated by the decrease in IGFBP 3 : IGF 1 ratio . ^^^ A significant correlation between IGF 1 and IGFBP 3 levels is present in the trauma patients who received TPN and rhGH . ^^^ A GH / IGF 1 / IGFBP 3 axis that closely regulates the metabolic status of the patient is established in trauma . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF studies indicated significant reductions in IGF 1 and elevated immunoreactive levels of IGFBP 3 in infected fetuses during the third trimester . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
They were also compared according to serum concentration of insulin like growth factor ( IGF ) 1 and IGF binding protein 3 , and age adjusted serum IGF 1 standard deviation score ( SDS ) , at entry into the study and by change in these measurements after 6 months of GH replacement . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the two groups of patients the age , height , weight , body mass index , serum insulin like growth factor ( IGF ) 1 , IGF binding protein ( IGFBP ) 3 and IGF 1 age matched standard deviation score ( SDS ) were compared at entry into the study , and changes in IGF 1 , IGFBP 3 and IGF 1 SDS were compared after 6 months of GH replacement . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , there was a highly significant correlation between IGFBP 3 and IGF 1 in pregnancy serum . ^^^ We conclude that , during pregnancy , IGFBP 3 appears to be the principal binding protein for circulating IGF 1 and may be involved in the regulation of fetal growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 , IGF 2 , and IGFBP 3 levels measured by RIA were 18 % , 14 % , and 16 % of those in serum , respectively . ^^^ Fast protein liquid chromoatography showed that both IGF 1 and IGFBP 3 in 150 and 50 kilodalton complexes were approximately 13 % and 37 % , respectively , of the corresponding peaks found in matched serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Twenty four hour blood samples were obtained at 10 min intervals and were used to determine GH pulsatility ( 20 min samples ) , IGFBP 3 levels ( 0800 0900 h ) , and fluctuations of insulin , IGFBP 1 , and IGF 1 ( hourly samples ) during feeding and fasting phases of the day . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels are increased in central precocious puberty : effects of two different treatment regimens with gonadotropin releasing hormone agonists , without or in combination with an antiandrogen ( cyproterone acetate ) . ^^^ It is generally believed that gonadal sex steroids stimulate pulsatile GH secretion , which , in turn , stimulates insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) production . ^^^ However , little is known about GH , IGF 1 , and IGFBP 3 serum levels in children with precocious puberty . ^^^ Treatment with GnRH analog in combination with cyproterone acetate significantly decreased height velocity and serum IGF 1 and IGFBP 3 levels to normal levels after 2 yr of treatment ( P < 0 . 0001 ) . ^^^ Serum IGF 1 levels remained unchanged during monthly im treatment with long acting GnRH analog , whereas IGFBP 3 levels significantly increased during the first year of this treatment despite unmeasurable estradiol levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The human keratinocyte cell line HaCaT has a basal phenotype and secretes an insulin like growth factor ( IGF ) binding protein , IGFBP 3 , which modulates its IGF 1 response . ^^^ We investigated the effects of IGF 1 and EGF , as well as three other skin growth regulators , retinoic acid , basic fibroblast growth factor , and dexamethasone , on mitogenic stimulation and IGFBP 3 production in HaCaT cells . ^^^ IGF 1 increased the level of IGFBP 3 in cell conditioned medium by up to twofold , whereas EGF caused a twentyfold reduction in IGFBP 3 . ^^^ IGF 1 stimulation of IGFBP 3 did not involve increases in IGFBP 3 mRNA ; however , EGF , consistent with its effect on IGFBP 3 protein , caused a fivefold reduction in IGFBP 3 mRNA . ^^^ In summary , EGF profoundly inhibited IGFBP 3 synthesis in basal keratinocytes , whereas IGF 1 increased IGFBP 3 levels by a posttranscriptional mechanism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that human bone cells ( HBCs ) , which produce abundant amounts of IGF 2 and IGFBP 3 , also produce ALS , we measured the IGF 1 , IGF 2 , IGFBP 3 , and ALS levels using specific radioimmunoassays ( RIAs ) in the conditioned medium ( CM ) of untransformed normal HBCs and SaOS 2 osteosarcoma cells treated with various effectors ( IGF 2 , osteogenic protein 1 [ OP 1 , bone morphogenetic protein 7 ] and human GH ) for 48 h . ^^^ In the IGF 2 ( 3 , 30 ng / ml ) treated HBC CM , the IGFBP 3 level was increased in a dose dependent manner but neither IGF 1 nor ALS could be detected . ^^^ In the SaOS 2 cell culture , OP 1 ( 1 , 100 ng / ml ) increased both IGF 2 and IGFBP 3 secretion but neither ALS nor IGF 1 secretion . ^^^ Treatment of HBCs with GH ( 1 , 10 , 100 ng / ml ) had no significant effect on the secretion of either IGF 1 , IGF 2 , IGFBP 3 , or ALS . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Elevated serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels have been found in patients with active acromegaly . ^^^ We have studied the relative diagnostic merits of measurements of IGFBP 3 compared with IGF 1 as a parameter of disease activity in these patients . ^^^ MEASUREMENTS : Twenty four hour sampling for serum GH in patients with acromegaly , serum IGF 1 and IGFBP 3 . ^^^ There was a considerable overlap for IGFBP 3 levels but not for IGF 1 levels , between normals and acromegalics . ^^^ There was a significant correlation between the mean 24 hour GH and IGFBP 3 levels ( P = 0 . 036 ) and between the IGF 1 and IGFBP 3 levels ( P < 0 . 002 ) in acromegaly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study investigates the regulation of IGFBP 3 phosphorylation by IGF 1 in human neonatal skin fibroblasts . ^^^ IGF 1 ( 50 ng / ml ) increased levels of P IGFBP 3 and 1 IGFBP 3 in conditioned medium to 205 + / 9 % and 198 + / 10 % of control , respectively ( n = 5 ) . ^^^ Stimulation of 1 IGFBP 3 was consistent with IGF mediated release of cell associated IGFBP 3 , since treatment with an IGF 1 analogue with reduced affinity for IGFBPs did not increase 1 IGFBP 3 levels , whereas treatment with an analogue with reduced affinity for receptor but normal affinity for binding proteins did . ^^^ In contrast , stimulation of P IGFBP 3 occurred independently of IGF binding to IGFBP , instead requiring interaction of IGF 1 with its receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : Baseline IGF 1 , insulin like growth factor binding protein 1 ( IGFBP 1 ) , IGFBP 3 , GH response to the exercise propranolol test , and insulin responses to a standard oral glucose tolerance test were compared among patients with functional ovarian hyperandrogenism , hyperandrogenic nonfunctional ovarian hyperandrogenism patients , and age matched controls . ^^^ RESULTS : Serum IGF 1 and poststimulated GH levels were similar among groups , whereas serum IGFBP 3 levels were significantly lower in group A than in controls . ^^^ Hyperinsulinemia and decreased IGFBP 3 levels may enhance IGF 1 bioavailability , which in turn may both decrease SHBG levels and increase androgen production . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGFBP 2 was elevated and IGF 1 was reduced in the nondiabetic Ax rats compared with sham Ax controls , but plasma IGFBP 3 and 4 were not significantly changed . ^^^ In the 0 STZ Ax rats , plasma IGF 1 and IGFBP 3 increased in proportion to the corticosterone implant dose , but IGFBP 1 was unaffected . ^^^ By contrast , IGF 1 and IGFBP 3 were unaltered by corticosterone in the 30 STZ Ax rats , and IGFBP 1 increased in proportion with the dose of corticosterone . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of serum insulin like growth factor 1 ( IGF 1 ) , IGF 1 binding protein 1 ( IGFBP 1 ) , and IGFBP 3 were related to specific measures of basal or pulsatile GH release . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Proteolysis of IGFBP 3 may result in the free form of IGF 1 ( fIGF 1 ) being liberated from or weakly bound to IGFBP 3 fragments . ^^^ We also measured plasma total IGF 1 ( free plus complexed forms of IGF 1 ) using immunoradiometric assay , and IGFBP 3 proteolytic activity using a IGFBP 3 protease assay . fIGF 1 levels at 6 8 weeks gestation ( 4 . 11 + / 0 . 23 ng / mL ) and at 28 30 weeks gestation ( 3 . 67 + / 0 . 21 ng / mL ) were significantly higher than that in nonpregnant women ( 2 . 48 + / 0 . 12 ng / mL ; P < 0 . 001 in both ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the ovary IGFBP 3 appeared to neutralize the actions of gonadotropin and IGF 1 , probably via its ability to sequester IGF 1 , in the process of follicular growth , oocyte maturation , and ovulation . ^^^ IGFBP 3 blocked the stimulatory effects of gonadotropin in the ovulatory process by neutralizing endogenously produced IGF 1 , resulting in reduced intrafollicular PA activities . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : GH response to GHRH in HA men was exaggerated compared to SL men , and IGF 1 concentration was also significantly increased in the presence of normal levels of IGFBP 3 . ^^^ CONCLUSIONS : This study is the first description of exaggerated GH response to the administration of GHRH in HA men and also of a significant increase in IGF 1 concentration in the same subjects in the presence of normal levels of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition to IGFBP 2 , a 35 45 kDa IGFBP identified by immunoprecipitation as IGFBP 3 was variably present on ligand blots of control conditioned medium and was consistently found in medium from cells treated with IGF 1 or IGF 2 ( ED 50 3 ng / mL ) but not with insulin at 2 mg / mL . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the mature animal , serum IGF 1 levels were partially corrected and IGFBP 3 levels were normalized by GH , but no change could be induced in the reduced serum IGFBP 4 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In lit / lit serum , overall binding of 125I labelled IGF 1 to the four IGFBPs was reduced , solely in response to a reduced amount of IGFBP 3 . ^^^ However , adult levels of IGF 1 were maintained in lit / + mice to at least 52 weeks of age , as were levels of IGFBP 1 and 4 , while IGFBP 3 and 2 declined with age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 ( adults ) and IGF binding protein 3 ( children and adults ) correlated inversely with statural deviation from normal ( P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This observation was associated with increased concentrations of IGF binding protein 3 and acid labile subunit on GH / IGF 1 treatment and decreased concentrations on IGF 1 alone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Interestingly , modifications in the A domain of IGF 1 , which is believed to contain residues critical for binding to IGF 1 and insulin receptors , show differential effects on binding affinity to BPs . [ Thr 49 , Ser 50 , Ile 51 ] IGF 1 , which has normal affinity for the type 1 IGF receptor , shows at least a 500 fold decreased affinity for hIGFBP 1 and recombinant hIGFBP 3 , in contrast to 50 to 100 fold reduced affinity for hIGFBP 2 and rIGFBP 4 , and 5 to 10 fold reduced affinity for purified human serum IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study examines responses of IGFBP 1 and the IGFBP 3 complex to recombinant human IGF 1 . ^^^ IGFBP 3 showed little response , decreasing slightly over the 48 h period following IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the major carrier of insulin like growth factor 1 ( IGF 1 ) in serum , and its production is growth hormone ( GH ) dependent . ^^^ It is unclear whether in humans IGFBP 3 production is directly regulated by GH or mediated via IGF 1 . ^^^ This proof of GH independent induction of IGFBP 3 by IGF 1 may be a major advantage in the therapeutic use of biosynthetic IGF 1 in several types of short stature children . . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the major carrier of insulin like growth factor 1 ( IGF 1 ) in serum , and its production is growth hormone ( GH ) dependent . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 levels determined by WLB and serum IGF 1 and IGF 2 levels measured by RIA after acid chromatography were not different among the subjects with cancer and the normal controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have confirmed that IR IGF 1 levels are low in hypothyroidism and have demonstrated a reduction in IGF bioactivity and IGF 2 and IGFBP 3 levels , and low GH binding activity , which may reflect a reduction in the processing of GH receptors . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The inhibitory effects of IGFBP 3 were greater for IGF 2 than for IGF 1 when IGFBP 3 was coincubated with either of the IGFs , at both IGF concentrations of comparable activity and equivalent molar concentrations . ^^^ Interestingly , when cells were pretreated with IGFBP 3 , prior to the simultaneous addition of IGFs and IGFBP 3 , the inhibitory effect was higher for IGF 1 than for IGF 2 . ^^^ IGFBP 3 exhibited an ED 50 low enough to suppress IGF 2 and possibly IGF 1 action when cells were pretreated with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , isolated GH deficiency results in : ( 1 ) reduced maternal weight gain and correspondingly smaller litter sizes ; ( 2 ) low and unchanged maternal serum IGF 1 levels ; ( 3 ) low , but declining , serum IGFBP 3 levels ; and ( 4 ) activity of IGFBP protease ( s ) detectable in early and late pregnancy , which may modulate the bioavailability and bioactivity of IGFs by regulating IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 ( 0 . 1 , 1 , and 2 . 5 pmol / ml ) caused a dose dependent inhibition of IGF 1 plus hCG induced testosterone formation . ^^^ IGFBP 3 in a concentration of 2 . 5 pmol / ml completely neutralized the effects of IGF 1 on Leydig cell steroidogenesis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All subjects had basal IGF 1 and IGFBP 3 levels prior to testing with GH releasing hormone [ 1 microgram / kg 4 bolus ; responses expressed as maximum percentage increase above baseline ( PERGH ) ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also studied the effects of hGH on osteoblastic production of insulin like growth factor 1 ( IGF 1 ) and IGF 2 as well as the production of GH dependent , insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Coincubation with equimolar amounts of IGF 1 and exogenous bovine IGFBP 3 potentiated the growth response observed with IGF 1 alone . ^^^ Cells expressing IGFBP 3 under basal conditions grew slower in serum , but were more responsive to 100 ng / ml of IGF 1 in serum free media compared to wild type MDBK cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 has approximately 1000 fold higher affinity than LR3IGF 1 towards IGFBP 3 , IGFBP 4 , total rat plasma IGFBPs and L 6 myoblast BP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , we examined whether IGF 1 induces hypertrophy in cultured neonatal rat cardiomyocytes through its specific receptor and whether IGF binding protein 3 ( IGFBP 3 ) , which is a major circulating carrier protein for IGF 1 , inhibits IGF 1 induced cardiac hypertrophy in vitro . ^^^ IGFBP 3 induced a dose dependent inhibition of protein synthesis stimulated by IGF 1 ; IGFBP 3 ( 10 ( 7 ) M ) completely inhibited the [ 3H ] leucine uptake stimulated by IGF 1 ( 10 ( 8 ) M ) . ^^^ IGFBP 3 similarly inhibited the IGF 1 stimulated gene expressions for MLC 2 and troponin 1 . ^^^ These results suggest that IGF 1 directly causes cardiac hypertrophy and that its effect can be blocked by IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 also increased the accumulated levels of IGFBP 3 in the conditioned media of both cell lines . ^^^ Both cell lines expressed high basal levels of IGFBP 3 mRNA ; the addition of RA increased IGFBP 3 mRNA levels by 1 . 5 fold , whereas the addition of IGF 1 had no effect on IGFBP 3 mRNA levels in either cell line . ^^^ Pretreatment of MDA MB 468 and MDA MB 231 cells with IGF 1 receptor antibody ( alpha IR 3 ) blocked the IGF 1 effect on IGFBP 3 levels in the media in both cell lines and IGFBP 2 and IGFBP 5 secreted levels in MDA MB 468 cell conditioned media . ^^^ Cycloheximide treatment completely blocked the RA and / or IGF 1 mediated modulation of these binding proteins , suggesting that these agents enhance IGFBP 3 , IGFBP 2 , and IGFBP 5 synthesis and consequent secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have quantified the effects of two cytokines , transforming growth factor beta 1 ( TGF beta 1 ) and tumour necrosis factor alpha ( TNF alpha ) which have opposing actions on fibroblast IGFBP 3 production , and examined their subsequent role in IGF 1 mitogenesis . ^^^ The IGF 1 analogue Long R 3 IGF 1 which has little affinity for the IGFBPs was approximately 20 fold more potent in this assay , and was unaffected by exogenous IGFBP 3 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Northern blots of total cellular RNA were probed with oligonucleotides for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and a human IGFBP 1 cDNA . ^^^ The data showed that in fibroids compared to myometrium , 1 ) the relative abundance of IGF 1 mRNA was not different , but there was an increase in the relative abundance of IGF 2 mRNA ( P < 0 . 001 ) ; 2 ) IGFBP 1 mRNA was undetectable in fibroids and detectable in only 1 specimen of myometrium ; 3 ) there was no difference in the relative abundance of IGFBP 2 mRNA , but there was an increase in the relative abundance of IGFBP 3 mRNA in myometrium ( P < 0 . 05 ) . ^^^ Our data show that the mRNAs encoding IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 are expressed in both fibroids and myometrium and that fibroids express more IGF 2 and less IGFBP 3 mRNA than myometrium . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 reflect spontaneous growth hormone secretion . ^^^ A significant correlation was found between the spontaneous GH secretion expressed as the area under the curve above baseline ( AUCb ) or the calculated 24 h GH secretion rate ( GHb ) and IGF 1 ( n = 147 , r = 0 . 65 , or 0 . 78 , respectively , P < 0 . 001 ) or IGFBP 3 levels ( r = 0 . 48 or 0 . 62 , P < 0 . 001 ) . ^^^ In prepubertal children , a significant correlation was found between height ( compared to Swedish reference values ) standard deviation scores ( SDS ) and IGF 1 SDS ( n = 75 , r = 0 . 66 , P < 0 . 001 ) or IGFBP 3 SDS ( r = 0 . 61 , P < 0 . 001 ) . ^^^ The reproducibility during repeated testing ( 19 individuals , 6 prepubertal ) was best with IGFBP 3 as compared to AUCb , GHb , or IGF 1 which showed considerable variability . ^^^ The data suggest : 1 ) that IGF 1 and IGFBP 3 serum levels reflect spontaneous GH secretion in healthy individuals ; 2 ) IGF 1 levels are more sensitive to GH regulation than IGFBP 3 ; 3 ) in puberty , there may be increased sensitivity of IGF 1 regulation by GH as compared to the prepubertal stage ; 4 ) short children have low IGF 1 and IGFBP 3 levels , whereas tall children have high levels , a fact which is likely to contribute to the understanding of height variability in a healthy population ; 5 ) the good reproducibility of IGFBP 3 on repeated testing makes it an interesting parameter for the evaluation of the GH IGF axis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to assess urinary IGF 1 , IGF 2 , and IGFBP 3 in a cross sectional study of healthy subjects , as well as characterize urinary IGFBPs ( uIGFBps ) in patients with GH deficiency ( GHD ) and renal disease , such as , Alport syndrome , immunoglobulin A nephropathy , focal segmental glomerulosclerosis , and systemic lupus erythematosus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All NHM cells and mesothelioma cell lines expressed IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and IGF 1 receptor mRNA by either Northern blot or reverse transcription polymerase chain reaction analysis . ^^^ IGF 1 ( 0 . 136 + / 0 . 024 ng / ml , mean + / SEM ) and IGFBP 3 ( 18 . 5 + / 3 . 2 ng / ml ) proteins were readily detected in the conditioned medium of mesothelioma cell lines but were not greater than corresponding measurements in that of NHM cells ( IGF 1 , 0 . 120 + / 0 . 080 ng / ml ; IGFBP 3 , 15 . 9 + / 1 . 3 ng / ml ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Immunoreactive IGFBP 3 was increased in proportion to IGF 1 and the molar ratio [ IGFBP 3 ] : [ IGF 1 ] + [ IGF 2 ] was close to unity ( 1 . 04 + / 0 . 14 ) , as that of healthy subjects . ^^^ Neutral size chromatography of serum from patients with Cushing ' s syndrome showed that IGF 1 and IGFBP 3 immunoreactivity were predominantly found at the elution volume of the ternary 150 kDa IGF I / IGFBP 3 / acid labile subunit complex and a similar pattern was displayed by normal serum . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels were low during fetal life and increased during postnatal development . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The mean fasting levels of glucose and insulin were higher , whereas the mean basal IGF 1 , IGF 2 , and IGFBP 3 levels were lower in the endometrial cancer patients than in the healthy control subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fetal growth plate chondrocytes primarily showed increases in IGFBP 3 and the 24 kDa form ( 4 . 7 and 2 . 7 fold , respectively ) in response to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the adult rat , expression of the liver GH receptor , insulin like growth factor 1 ( IGF 1 ) , and IGF 1 binding protein 3 ( IGFBP 3 ) genes has been shown to be under GH control . ^^^ To further elucidate the time of appearance and the extent of GH control of liver GH receptor , IGF 1 , and IGFBP 3 gene expression , we studied the effect of hypophysectomy and GH and IGF 1 treatment in juvenile rats . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The failure to maintain high serum IGF 1 levels overnight , presumably due to a persisting defect in serum IGFBP 3 levels , was associated with an early return of GH secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Pretreatment plasma / serum levels of GH , IGF 1 , binding proteins for GH ( GHBP ) and IGF 1 ( IGFBP 3 ) were used as a basis for comparison of the levels found after each regimen . ^^^ MEASUREMENTS : Plasma levels of GHBP by standardized binding assay ; GH , IGF 1 , and IGFBP 3 serum / plasma levels by radioimmunoassay . ^^^ There was a significant increase in levels of IGF 1 and IGFBP 3 , irrespective of regimen . ^^^ The IGF 1 to IGFBP 3 ratio , a possible indicator of the growth response , rose significantly and comparably with both regimens . ^^^ There was no consistent diurnal variation with either regimen in GHBP , IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine 1 ) the time course of the decrease in insulin sensitivity in relationship to Tanner stage of genital development , and 2 ) how this change relates to changes in GH secretion , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and gonadal steroid secretion , we studied 58 healthy children and adolescents ( 34 males and 24 females ; age 7 15 yr ) using overnight GH sampling and frequently sampled 4 glucose tolerance tests . ^^^ IGF 1 and IGF binding protein 3 followed opposite patterns to ISI , with lower levels in Tanner stage 1 than in stages 2 5 ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Cleavage of IGFBP 3 results in the appearance of a 31 kDa fragment that is 50 fold reduced in its affinity for the IGF 1 or IGF 2 . ^^^ Specifically , the binding of IGFBP 3 to cell surfaces is associated with its ability to enhance IGF action and with a ten to 12 fold reduction in its affinity for IGF 1 and IGF II . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After 24 h of treatment , IGF 1 , IGF 2 , and high concentrations of insulin increased IGFBP 2 and IGFBP 3 levels . ^^^ Time course studies demonstrated that IGF 1 rapidly induced the mRNA levels of both IGFBP 2 and IGFBP 3 within 7 . 5 12 h . ^^^ In situ hybridization studies demonstrated an increase in the abundance per cell of IGFBP 2 and IGFBP 3 mRNAs in either one or both types of astroglial cells after IGF 1 or IGF 2 treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) forms a 140 kilodalton ( kDa ) complex with an acid labile glycoprotein after complexing with IGF 1 or IGF 2 . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) forms a 140 kilodalton ( kDa ) complex with an acid labile glycoprotein after complexing with IGF 1 or IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The somatotropin somatomedin axis in adult patients with Turner syndrome : measurement of stimulated GH , GH BP , IGF 1 , IGF 2 and IGFBP 3 in 25 patients . ^^^ No significant differences were present when the integrated GH response to stimulation with arginine and the serum levels of GH binding protein ( GH BP ) , IGF 1 , IGF 2 and binding protein 3 for IGFs ( IGFBP 3 ) were compared between the two groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 6 patients with GH deficiency and in 5 patients with GHIS , the pharmacokinetic profile of IGF 1 after a single injection was strongly dependent on the IGFBP 3 concentration . ^^^ A slight but significant increase in IGFBP 3 was observed coincident with the IGF 1 peak , whereas IGFBP 2 increased after a delay of about 10 hours . ^^^ In the patients with GHIS , chronic IGF 1 treatment , with twice daily injections for 6 months , caused a significant steady decline of IGF 2 and an increase in IGFBP 2 , but had no effect on IGFBP 1 and IGFBP 3 levels . ^^^ The data suggest that total IGF 1 and IGF 2 serum levels are determined mainly by IGFBP 3 , even in extreme situations such as GHIS , while other IGFBPs are less important . ^^^ The IGFBP 3 concentration seems to be a major regulator of the pharmacokinetics of exogenous IGF 1 , which , in turn , influences IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition to old and young cultures of recombinant human IGFBP 3 and IGF 1 at 1 : 1 or 5 : 1 molar ratios inhibited IGF 1 mediated DNA synthesis by approximately 70 80 % . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our findings indicate that low levels of circulating IGF 1 in Perthes ' disease , as we have reported previously , are caused neither by altered concentrations of the principal IGF binding protein , IGFBP 3 , nor by an underlying growth hormone deficiency . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH every 20 min for 24 h , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and estradiol ( E 2 ) were measured in a 7 . 3 yr old girl with precocious puberty due to McCune Albright syndrome ( MAS ) who developed stigmata of early acromegaly and in 9 other MAS patients who had no signs of acromegaly . ^^^ We found no differences in mean GH , GH pulse frequency , pulse height , or pulse area between MAS patients and controls except in patient 1 , who had an elevated mean + / SD GH compared with controls ( 15 . 4 + / 2 vs . 4 . 8 + / 2 . 3 micrograms / L ; P < 0 . 01 ) and an elevated IGF 1 ( 908 micrograms / L ) and IGF binding protein 3 ( 5 . 6 mg / L ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When the cells were incubated with IGF 1 , IGFBP 3 and 4 concentrations increased , but IGFBP 2 production was much less stimulated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the 150 kilodalton IGFBP complex were decreased before and restored to normal during treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURES : Follicle number and diameter , as well as endometrial thickness were all assessed by vaginal ultrasound and blood was obtained for serum FSH , LH , E 2 , P , GH , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have evaluated the relative diagnostic merits of measuring peak GH response to insulin induced hypoglycaemia ( insulin tolerance test ) , mean 24 h GH concentration derived from 20 min sampling , serum insulin like growth factor 1 ( IGF 1 ) concentrations , and serum IGF binding protein 3 ( IGFBP 3 ) concentrations . ^^^ Hypopituitary subjects had significantly lower stimulated peak GH , mean 24 h GH , IGF 1 , and IGFBP 3 concentrations than normal subjects . ^^^ The ranges of stimulated peak GH responses were clearly separated between the hypopituitary ( < 0 . 2 3 . 1 ng / mL ) and normal ( 5 . 3 42 . 5 ng / mL ) groups , but mean 24 h GH , IGF 1 , and IGFBP 3 concentrations overlapped . ^^^ Mean 24 h GH concentrations were below assay sensitivity in 80 % of hypopituitary subjects and 16 % of normal subjects . 70 % and 72 % , respectively , of the IGF 1 and IGFBP 3 values in hypopituitary subjects were within the range for normal subjects . ^^^ We conclude that GH deficiency in adults is most reliably identified by stimulatory testing , and that IGF 1 and IGFBP 3 are poor diagnostic tests of adult GH deficiency . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The new criteria were : height below 3 SDS , basal GH 4 mU / l or above , GH binding below 10 % , basal IGF 1 and basal IGF binding protein 3 ( IGFBP 3 ) below the 0 . 1 centile for age , an increase of IGF 1 in the IGF generation test less than 15 micrograms / l , and the increase of IGFBP 3 less than 0 . 4 mg / l . ^^^ IGFBP 3 was included to give the GH dependent parameters of the IGF system more weight and because the accuracy of IGFBP 3 in the IGF generation tests was greater than the accuracy of IGF 1 , when the group of patients with GHIS was compared with a group of patients with GH deficiency . ^^^ In the GHIS positive group , a significant correlation was found between basal IGF 1 or IGFBP 3 levels corrected for age ( SDS ) and height SDS ( r = 0 . 49 , p < 0 . 002 and r = 0 . 61 , p < 0 . 0001 , respectively ) . ^^^ There was also a significant correlation between the changes of IGF 1 or IGFBP 3 in the IGF generation test and height SDS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It has previously been demonstrated that 17 parents heterozygous for the Ecuadorean mutation of the GH receptor differed little in biochemical measures ( GHBP , IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 ) from Ecuadorean controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a subgroup of nine patients with peak GH levels of 63 . 7 + / 3 . 7 mU / l during a glucagon tolerance test , the response to the IGF 1 generation test was no different from that for the group as a whole ( peak GH , 43 . 3 + / 4 . 5 mU / l ) , despite the fact that this subgroup exhibited a negative relationship between height SDS and peak GH and a positive relationship between height SDS and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We attributed these results to the large increase in IGFBPs that occurred with maturation , as well as an induction of IGFBP 3 ( 34 68 % ) and a larger increase in the 30 34 kDa IGFBP ( BP 2 ; 136 235 % ) following IGF 1 treatment in the older ( 16 24 months ) animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied plasma gonadotropins , sex steroids , adrenal androgens , and insulin like growth factors ( IGF 1 and IGF 2 ) and their binding protein 3 ( IGFBP 3 ) in a large number of WBS patients from infancy to adulthood ( n = 23 females , n = 33 males ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Therapy with rhGH ( up to 0 . 5 mg / kg . week ) did not improve growth and failed to increase the levels of circulating IGF 1 and IGF binding protein 3 over a 14 month period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Though IGF 1 mRNA levels do not increase after MSBR , the precipitous and early fall in ileal IGF binding protein 3 ( IGFBP 3 ) mRNA levels suggests a fall in IGFBP 3 levels may increase local IGF 1 bioactivity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The different affinities of des ( 1 3 ) IGF 1 for IGFBP 3 , 2 , and 1 lead us to suggest that IGFBP 2 and / or 1 are present in the deep stroma and glands , whereas IGFBP 3 may be responsible for the additional binding sites in the myometrium and blood vessel walls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of serum IGF 1 , IGFBP 3 , insulin and blood glucose were measured to study the short term metabolic effects of growth hormone . ^^^ Similar levels of serum IGF 1 , IGFBP 3 , insulin and blood glucose were obtained . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Human seminal plasma contains IGF 1 and 2 , IGFBP 2 and 4 , as well as IGFBP 3 fragments and IGFBP 3 protease activity . ^^^ This IGFBP 3 protease activity can remove the inhibitory effects of IGFBP 3 on IGF 1 induced PC 3 growth . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE We examined the effects of different doses of GH on insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , body composition , energy expenditure , and various metabolites in GH deficient adults , in order to approach a metabolically appropriate GH dosage in young GH deficient adults . ^^^ CONCLUSION Based primarily on IGF 1 and IGFBP 3 levels our data suggest that a GH replacement dose in young GH deficient adults in the order of 1 2 IU / m2 per day is adequate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal serum levels of hGH were high and IGF 1 low , and in contradistinction to the classical form of the disease serum GHBP and IGFBP 3 were normal in these patients . ^^^ It is concluded that the findings described are compatible with a normal GH receptor and normal signal transmission for IGFBP 3 synthesis but a defect exists in the post GH receptor mechanism for the generation of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To further our understanding of the growth failure in metabolic acidosis , we examined the insulin like growth factor ( IGF 1 and IGF 2 ) , the IGF binding protein 3 ( IGFBP 3 ) , and the hepatic IGF mRNA and growth hormone receptor mRNA in control , pair fed and acidotic rats . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Undifferentiated P 19 cells synthesized IGF 1 and 2 , the type 1 and 2 IGF receptors , and IGF binding proteins ( IGF BP 2 , IGF BP 3 , and IGF BP 4 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In healthy individuals , spontaneous and stimulated GH secretion , as well as circulating IGF 1 and IGFBP 3 levels , are significantly decreased with advancing age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As expected , IGF 1 and IGF 2 concentrations in serum correlated positively with the serum concentration of IGFBP 3 , the major IGF binding protein in serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The increase in IGF 1 levels was accompanied by a significant increase in IGF binding protein 2 levels , a slight reduction in IGF binding protein 3 levels , and an increase in levels of IGF binding protein 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A continuous decline in the concentrations of IGF 1 , IGFBP 3 and serum GH binding activity ( GHBP ) was observed during progression of cirrhosis and the data correlated significantly with choline esterase , total serum protein and the Child score . ^^^ The excellent correlation between the serum levels of IGF 1 ( r = 0 . 64 , p < 0 . 001 ) or IGFBP 3 ( r = 0 . 67 , p < 0 . 001 ) and the Child score index suggests that they reflect the hepatic functions just as conventional indicators . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report that while TNF alpha had no consistent effect on the levels of IGF 1 , IGF 2 , or on IGF 1 receptor ( protein and messenger RNA ( mRNA ) ] , it stimulated IGFBP activity and particularly IGFBP 3 . ^^^ Such an increase in IGFBP 3 in TNF alpha treated Sertoli cells results probably in a decrease in IGF 1 bioavailability for its receptors and thus in a decrease in IGF 1 action . ^^^ Indeed , addition of recombinant human IGFBP 3 ( 10 nM ) suppressed completely the stimulatory addition of IGF 1 ( 3 nM ) on FSH binding to cultured porcine Sertoli cells . ^^^ Together , the present findings indicate that , in Sertoli cells , TNF alpha antagonizes IGF 1 action through the modulation of IGFBPs and particularly through an increase in IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During pregnancy in the baboon , this ratio is perturbed ; serum IGFBP 3 concentrations increase 10 fold , yet IGF 1 levels are unaltered and IGF 2 is increased only 2 fold ( Giudice et al . 1993 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study we measured the concentrations of IGF 1 , IGF 2 , and IGFBP 3 in normal human synovial fluid by RIA and characterized the IGFBPs by Western ligand blot ( WLB ) , Western immunoblot , and immunoprecipitation . ^^^ The concentrations of IGF 1 , IGF 2 , and IGFBP 3 in normal synovial fluid were 19 + / 3 ( mean + / SE ) , 194 + / 14 , and 349 + / 65 ng / mL , respectively . ^^^ In synovial fluid of patients with OA , IGF 1 levels were elevated , whereas IGF 2 was decreased , and the IGFBP 3 level was similar to the control value . ^^^ In patients with RA , both IGF 1 and IGFBP 3 were elevated , whereas IGF 2 remained unchanged . ^^^ In pathological states , the increased IGF 1 concentrations were accompanied by an increase in IGFBP 1 concentrations were accompanied by an increase in IGFBP 3 levels in synovial fluid . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The bulk of circulating insulin like growth factor 1 ( IGF 1 ) and its major circulating binding protein , IGF binding protein 3 ( IGFBP 3 ) , is derived from the liver . ^^^ Serum IGF 1 , IGFBP 1 , and insulin were measured by RIA , and IGFBP 3 was determined by immunoradiometric assay in 26 children with ESLD ( mean of 3 . 7 samples pre OLT and 4 . 2 samples post OLT per patient ) and 30 age matched controls . ^^^ Pre OLT , IGF 1 ( 32 . 7 + / 4 . 8 micrograms / L ) , and IGFBP 3 ( 1 . 11 + / 0 . 10 mg / L ) were significantly lower than control values [ IGF 1 , 168 . 3 + / 16 . 5 micrograms / L ( P = 0 . 0001 ) ; IGFBP 3 , 2 . 57 + / 0 . 17 mg / L ( P = 0 . 0001 ) ] . ^^^ Post OLT , IGF 1 ( 179 . 2 + / 19 . 7 micrograms / L ; P = NS ) rose to control levels , whereas IGFBP 3 ( 3 . 49 + / 0 . 14 mg / L ; P = 0 . 002 ) became significantly greater than the control value . ^^^ In conclusion , IGF 1 and IGFBP 3 are reduced , and IGFBP 1 and IGFBP 2 are increased in children with ESLD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone ( GH ) secretory dynamics in a case of acromegalic gigantism associated with hyperprolactinemia : nonpulsatile secretion of GH may induce elevated insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels . ^^^ We describe a case of pituitary gigantism with low levels of growth hormone ( GH ) , elevated insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGF BP 3 ) . ^^^ Serum prolactin , IGF 1 and IGF binding protein 3 levels were elevated . ^^^ In the present case , nonpulsatile GH secretion and enhanced tissue sensitivity to GH may induce hypersecretion of IGF 1 and IGF BP 3 and cause clinical acromegalic gigantism . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patients were compared to normal men by investigating GH sampling every 10 min for 24 h and serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and GH binding protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fetal blood samples ( FBS ) were collected for complete blood counts ( CBCs ) , hematopoietic progenitor assay , circulating insulin like growth factors ( IGF 1 , IGF 2 ) and binding proteins ( IGFBP 3 ) ( GD 120 , 140 , 153 + / 1 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , IGF 1 ( 10 ng / ml ) increased secretion of IGFBP 3 and 5 , whereas FSH ( 200 ng / ml ) decreased IGFBP 3 secretion and increased IGFBP 4 secretion ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
An important role for IGF 1 in colonic adaptation after massive intestinal resection is indicated by 1 ) significantly enhanced colonic mucosal growth and water absorption with IGF 1 treatment and 2 ) postresection upregulation of colonic IGF 1 mRNA and alteration of IGFBP 3 and IGFBP 4 mRNA expression . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : FSH enhances the action of IGF 1 in human granulosa cells by inhibiting the secretion of IGFBP 1 and the binding activity of IGFBP 3 by stimulating the proteolysis of IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGF 2 in Hyp mice were not significantly different from those in normal mice , but IGFBP 3 levels were significantly lower ( 70 % of normal , p < 0 . 05 ) in the mutant strain . ^^^ Phosphate supplementation normalized serum phosphate levels in Hyp mice and elicited a significant decrease in serum IGF 1 levels ( 23 % , p < 0 . 05 ) and a further deduction in IGFBP 3 ( 22 % , p < 0 . 02 ) . ^^^ Based on the documented effects of parathyroid hormone ( PTH ) on IGF 1 and IGFBP 3 , we propose that the secondary hyperparathyroidism displayed by Hyp mice and its exacerbation by phosphate supplementation may contribute to low IGFBP 3 levels in control Hyp mice and to the decreases in serum IGF 1 and IGFBP 3 in phosphate supplemented Hyp mice . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These effects of IGF 1 on follicular growth and oocyte maturation were significantly inhibited by IGFBP 3 ( 100 ng / ml ) . ^^^ Furthermore , IGFBP 3 significantly inhibited the IGF 1 stimulated production of E 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During regeneration following folic acid induced acute renal failure , IGF 1 , IGFBP 3 , and IGFBP 5 mRNAs declined in abundance approximately two to threefold . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both hormone infusions increased the amounts of IGF 1 in serum , IGF 1 mRNA in liver , and IGF binding protein 3 in serum and decreased IGF binding protein 2 in serum , but these effects were less for cows given releasing factor than for those given bST . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At the end of the 7 week period , the animals were killed . rhGH and rhIGF 1 increased serum GH and IGF 1 levels , respectively ; rhGH and rhIGF 1 in combination induced the highest serum IGF 1 levels ( 906 + / 261 ng / ml vs . control , 185 + / 36 ng / ml at death ) . rhGH and rhIGF 1 also increased IGFBP 3 levels . rhGH infusion resulted in the most marked changes in lymph node and splenic reactivity , as determined by histological assessment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fetal urinary IGF 1 and IGFBP 3 are increased in severe fetal bilateral obstructive uropathy , possibly reflecting tubular dysfunction or / and increased synthesis consequent upon fetal kidney injury . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the ovine fetus , insulin like growth factor 1 ( IGF 1 ) is bound mainly to the low molecular weight insulin like growth factor binding proteins ( IGFBP ) , IGFBP 1 and IGFBP 2 , with little binding to IGFBP 3 until near term at 147 days gestation . ^^^ To determine if there was any difference in plasma IGF 1 and IGFBP 3 concentrations in growth retarded fetal sheep with altered renal status , concentrations were measured by specific radioimmunoassay from bilaterally nephrectomized fetal sheep between Days 113 and 135 gestation . ^^^ In nephrectomized fetal sheep , increased IGFBP 3 concentrations , and therefore increased IGF binding capacity in fetal plasma , may have contributed to a decrease in free IGF in plasma and decreased IGF 1 bioactivity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In PRL and GH deficient rats , PRL treatment could prevent all of these effects , GH was partially effective , whereas putative mediators of GH action , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 3 , were ineffective . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Previous work indicate that glucocorticoids inhibit the synthesis of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IG FBP 3 ) , 4 , and 5 , but not IGFBP 6 , in osteoblast cultures . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) circulates in plasma predominantly in a 150 kDa complex together with IGF binding protein 3 ( IGFBP 3 ) , an approximately 40 kDa glycoprotein that binds IGF 1 with high affinity , and an 85 kDa acid labile subunit that does not bind IGFs . ^^^ Proteolysis of insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) in 150 kilodalton IGFBP complexes by a cation dependent protease activity in adult rat serum promotes the release of bound IGF 1 . ^^^ The present study demonstrates that proteolysis of IGFBP 3 in the 150 kDa complex occurs in vitro and results in the release of IGF 1 . ^^^ Proteolysis of human IGFBP 3 ( hIGFBP 3 ) also occurred within 150 kDa complexes when ternary complexes that had been reconstituted from recombinant hIGFBP 3 , rat acid labile subunit , and IGF 1 were incubated with rat serum . ^^^ Similar proteolysis of IGFBP 3 within 150 kDa complexes in vivo would provide a mechanism for mobilizing IGF 1 from the circulating reservoir in plasma as well as for the turnover of IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
One possible mechanism of action of rhGH in uremia is the restoration of circulating IGF bioactivity , which results from the differential regulatory effect of rhGH on circulating IGF 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The liver is the major source of IGF 1 and its binding protein , IGFBP 3 . ^^^ Circulating concentrations of IGF 1 and IGFBP 3 are decreased in patients with chronic liver disease and correlate with the severity . ^^^ The aim of this study was to assess the additional prognostic value of IGF 1 and IGFBP 3 in patients entered in a large multicenter study ( EMALD ) . ^^^ When IGF 1 or IGFBP 3 were added into this model , a Cox regression analysis showed that either had a significant independent prognostic value . ^^^ Because IGF 1 and IGFBP 3 were closely correlated , they contained almost the same prognostic information . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , the suppressive effect of FSH on apoptosis was partially reversed by coincubation with IGF binding protein 3 , suggesting a potential mediatory role of endogenous IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 levels were not correlated with primary renal diseases or the amount of proteinuria . ^^^ We speculate that low serum IGF 1 and IGFBP 3 levels would be partially due to the increased urinary losses of serum IGF IGFBP complexes , especially that of 150 kDa , and these changes may contribute to growth failure in persistent nephrotic syndrome . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 concentrations compared to stimulated and night growth hormone in the evaluation of short children a clinical research center study . ^^^ To evaluate the relative usefulness of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in screening for GH status , GH stimulation ( arginine insulin / L DOPA ) tests and overnight GH studies ( every 20 min sampling ) were performed in 104 healthy short children ( 32 girls ) , aged 3 16 yr ( height , 1 . 8 or more SD ) . ^^^ IGFBP 3 had no advantage over IGF 1 in screening sensitivity or specificity . ^^^ Both IGF 1 and IGFBP 3 correlated with peak stimulated GH . ^^^ To identify more than 90 % of children with GH deficiency ( GHD ) and borderline GHD , the mean values for age for IGF 1 and IGFBP 3 were required as the cut off criterion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
On the other hand , approximately 90 % of patients with normal IGF binding protein 3 levels among partial GH deficiency and normal short children had free and total IGF 1 levels above the 5th percentile for age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Positive correlations were found between follicular fluid IGF 1 and IGF binding protein 3 on the one hand and androgens on the other . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Erythropoiesis was investigated in 32 children wih short stature and in eight children with skeletal dysplasia by studying blood hemoglobin in relation to growth and to serum concentrations of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and erythropoietin ( EPO ) before , during , and after 12 months of recombinant human growth hormone ( GH ) treatment . ^^^ Blood hemoglobin concentration was positively correlated with relative body height and with serum IGF 1 and IGFBP 3 levels ( P = . 001 to . 02 ) , but not with the concentrations of EPO . ^^^ Treatment with GH accelerated growth and elevated the concentrations of hemoglobin , IGF 1 , and IGFBP 3 . ^^^ In this group , the correlations between hemoglobin , IGF 1 , and IGFBP 3 were extremely close ( r = 0 . 80 to 0 . 85 , P = . 031 to . 008 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No detectable IGFBP 3 was observed by WLB with [ 125I ] IGF 1 as the ligand , but with IGF 2 as the ligand , IGFBP 3 fragments at 16 17 kDa were noted . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) were measured in 46 GHD men participating in a 1 yr , double blind , and placebo controlled dose response study . ^^^ Significant increases in IGF 1 , IGFBP 3 , and ALS levels were observed with a dose as low as 0 . 33 IU / M ( 2 ) day . ^^^ The minimal GH dose required for normalization of the serum IGF 1 concentration was 0 . 66 IU / M ( 2 ) day , and it was 1 . 0 IU / M ( 2 ) day for ALS and IGFBP 3 . ^^^ In patients receiving 2 . 0 IU / M ( 2 ) day , the mean serum IGF 1 concentration rose to an abnormally high level , whereas at this dose , the mean IGFBP 3 and ALS levels were not different from normal . ^^^ The number of patients who developed IGFBP 3 or ALS levels that exceeded the upper normal limit was substantially smaller than the number of patients with elevated IGF 1 concentrations ( 2 , 8 , and 19 of 46 patients , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine the role of the insulin like growth factors ( IGFs ) in this process we developed a transgenic model using the whey acidic protein ( WAP ) gene to direct expression of rat IGF 1 and human IGF binding protein 3 ( IGFBP 3 ) to mammary tissue during late pregnancy and throughout lactation . ^^^ High levels of expression of transgenic IGF 1 and IGFBP 3 were seen in lobular alveolar cells by in situ hybridization . ^^^ We also evaluated the role of IGF 1 and IGFBP 3 in the remodeling of mammary tissue during involution . ^^^ These results demonstrate that IGF 1 and IGFBP 3 may modulate the involutionary process of the lactating mammary gland . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When cells were preincubated for 48 h with 50 nM recombinant human ( rh ) IGFBP 3 , IGF 1 stimulated [ 3H ] aminoisobutyric acid ( [ 125H ] AIB ) uptake was enhanced 2 to 3 fold . ^^^ The addition of cytoskeletal disrupting agents during the preincubation with rhIGFBP 3 did not affect IGFBP 3 potentiation of IGF 1 action , nor did a variety of serine , aspartate , and metalloproteinase inhibitors . ^^^ On the other hand , ammonium chloride and chloroquine , weak bases that neutralize the pH of acidic cell compartments , blocked IGFBP 3 potentiation of IGF 1 stimulated [ 3H ] AIB uptake . ^^^ Bafilomycin A , a specific inhibitor of ATP dependent hydrogen ion pumps , also inhibited IGFBP 3 potentiation of IGF 1 stimulated [ 3H ] AIB uptake . ^^^ Competitive [ 125I ] IGF 1 binding and affinity cross linking experiments suggested structure / function changes in cell bound IGFBP 3 that were altered in the presence of chloroquine and bafilomycin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Angiotensin 2 markedly reduced plasma IGF 1 levels ( 56 and 41 % decrease at 1 and 2 wk , respectively ) and IGF binding protein 3 levels , and increased IGF binding protein 2 levels , a pattern suggestive of dietary restriction . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At randomization and after 12 weeks body weight , body composition , thyroid hormones , catecholamines , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 were measured . ^^^ No differences were found for thyroid hormones , catecholamines , IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURES : Serum E 2 , FSH , GH , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 concentrations . ^^^ Serum IGFBP 3 levels increased 2 days after IGF 1 levels in the 12 IU human GH group only . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone treatment increased GH , IGF 1 , the 28 kDa IGFBP , and the 40 to 43 kDa IGFBP ( IGFBP 3 ) ( P < . 004 or P < . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with insulin or des ( 1 3 ) IGF 1 resulted in the appearance of IGFBP 3 in conditioned media and on the cell surface . ^^^ Thus , RA is a potent but transient inhibitor of bovine mammary epithelial cell proliferation , and this growth inhibition is correlated with increased IGFBP 2 accumulation and inhibition of IGF 1 stimulated IGFBP 3 protein secretion . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 levels are increased fivefold by retinoic acid , eightfold by dexamethasone , and tenfold by IGF 1 . ^^^ DP cells are known to produce IGF 1 , and so the observed stimulation of DP cell IGFBP 3 production by IGF 1 is consistent with the idea that DP cells possess the IGF transmembrane receptor kinase and are autoregulated by IGFs . ^^^ The IGFBP 3 , in turn , forms a complex with free IGF 1 to reduce the concentration of IGF 1 available to stimulate hair elongation and maintenance of anagen phase . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 screening for disorders of growth hormone secretion . ^^^ To assess the value of plasma assays of insulin like growth factor ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in the diagnosis of growth hormone disorders in children and adults . ^^^ Plasma IGF 1 and IGFBP 3 were measured in 47 children referred for the assessment of short stature , 26 adult subjects with hypopituitarism and in 10 adult subjects with acromegaly . ^^^ Six of 47 short children had growth hormone deficiency and in these cases both IGF 1 and IGFBP 3 were close to the lower limit or below the normal reference range . ^^^ In young children ( < 10 yr ) IGFBP 3 was more informative than IGF 1 , distinguishing normal short children from those with growth hormone deficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Swapping of the disulfide bridges in IGF 1 and the C region mutants decreased the affinity dramatically for IGFBP 3 , primarily by decreasing the association rate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the control group , puberty occurred at about 120 days of age and was associated with an increase in concentrations of testosterone , IGF 1 and IGFBP 3 and a decrease in concentration of IGFBP 2 . ^^^ In conclusion , concentrations of testosterone , IGF 1 and IGFBP 3 increase together , but probably independently , during the onset of puberty in male calves . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Proteoglycan synthesis under insulin like growth factor 1 ( IGF 1 ) stimulation was studied after a pulse of 35SO4 ( 2 ) in the presence or absence of added IGFBP 3 . ^^^ IGF 1 induced a 20 fold increase and IL 1 a 2 fold increase in IGFBP 3 release . ^^^ Co incubation of IGF 1 with recombinant ( r ) IGFBP 3 did not affect the rate of PG synthesis . ^^^ This study demonstrates that the ability of IGF 1 to enhance proteoglycan synthesis varied among the OA samples and may in part be dependent on the local level of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentrations of IGF 1 and IGFBP 3 in media obtained from the culture of embryos alone , cells alone or cells plus embryos were determined by radioimmunoassays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and its binding protein IGFBP 3 , both of which are induced by hGH , were also elevated for four weeks by the rhGH containing microspheres to a level greater than that induced by the same amount of rhGH administered by daily injections . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Obese Zucker rats maintain normal rates of linear growth and circulating concentrations of insulin like growth factor 1 ( IGF 1 ) and of IGF binding protein 3 ( IGFBP 3 ) in spite of low GH secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Estradiol and triiodothyronine increase production of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) by GH4C1 rat pituitary tumor cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the effects of growth hormone ( GH ) , prolactin ( PRL ) and insulin like growth factor 1 ( IGF 1 ) on DNA synthesis , and insulin like growth factor binding protein 3 ( IGFBP 3 ) gene expression , in a rat beta cell line INS 1 . ^^^ IGF 1 also stimulated DNA synthesis , but its effect on IGFBP 3 mRNA accumulation was moderate . ^^^ The GH activities in this cell line were not mediated by IGF 1 production : ( 1 ) no IGF 1 mRNA could be detected in poly A RNA preparation , and IGF 1 level was very low ; ( 2 ) neither IGF 1 mRNA nor the protein , were increased upon GH treatment ; ( 3 ) DNA synthesis stimulatory activities of GH and IGF 1 were additive ; ( 4 ) the GH induced IGFBP 3 mRNA increase was not inhibited by cycloheximide treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 , but not insulin , led to a much higher concentration of IGFBP 3 in the CM of all tested NSCLC cell lines , whereas the level of immunologically detected membrane associated IGFBP 3 was decreased . ^^^ Furthermore , Northern analysis of mRNA isolated from A 549 revealed that IGFBP 3 specific mRNA was not changed by IGF 1 or IGF 2 , suggesting that the IGF induced effects on IGFBP 3 depend on the release of cell associated IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To analyse which of them is the main cause of GH IGF 1 axis alterations , serum levels of growth hormone ( GH ) , growth hormone releasing factor ( GHRH ) , IGF 1 and its binding protein IGFBP 3 were measured in 85 hospitalized alcoholics ( 51 without cirrhosis , 15 with compensated cirrhosis and 19 with cirrhosis with ascites ) and in 25 healthy controls . ^^^ Serum IGF 1 and IGFBP 3 levels were lower in alcoholics , particularly in those with liver cirrhosis . ^^^ However , there was a significant positive correlation between serum GH concentrations and impaired liver function and a significant negative correlation between serum IGF 1 and IGFBP 3 and impaired liver function . ^^^ This suggests that , in this population , serum IGF 1 and IGFBP 3 levels reflect liver dysfunction rather than malnutrition . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Serum insulin like growth factor 1 ( IGF 1 ) and serum IGF binding protein 3 ( IGFBP 3 ) levels increased significantly during GH treatment , as did seminal IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and the analogs of IGF 1 ( LR 3 and Des [ 1 3 ] ) induced a dose dependent increase in both proliferation and IGFBP 3 production . ^^^ This was confirmed by the ability of excess recombinant IGFBP 3 to inhibit the IGF 1 and Des [ 1 3 ] induced proteolysis of IGFBP 4 . ^^^ Addition of IGF 1 to media from cells unexposed to IGF induced IGFBP 4 proteolysis but this was not seen with LR 3 which does not bind to IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 increased the abundance of IGFBP 3 , 4 and 5 in TC 1 conditioned medium ; this correlated with induction of IGFBP 3 mRNA , but not with that of IGFBP 4 or 5 mRNAs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and TGF beta were potent inducers of the release of IGFBP 3 protein ; however , TGF beta , but not IGF 1 , increased IGFBP 3 mRNA levels . ^^^ Recombinant IGFBP 3 was tested for its effect on IGF 1 induced mitogenesis . ^^^ IGFBP 3 inhibited IGF 1 stimulated DNA synthesis in a dose dependent manner with a peak effect observed at 50 nM IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Maternal milk production and plasma concentrations of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and growth hormone ( GH ) were determined before starting treatment and 1 day after stopping therapy . ^^^ Plasma concentrations of IGF 1 and IGFBP 3 increased in hGH treated mothers but not placebo treated mothers ; there was no change in plasma GH levels in either group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Developmentally regulated expression of IGF binding protein 3 ( IGFBP 3 ) in human placental fibroblasts : effect of exogenous IGFBP 3 on IGF 1 action . ^^^ The ability of IGFBP 3 to influence IGF 1 stimulated DNA synthesis was studied in 10 week placental fibroblasts as measured by [ 3H ] thymidine incorporation . ^^^ IGFBP 3 inhibited IGF 1 ( 3 . 3 nM ) stimulated DNA synthesis in a dose dependent manner when added simultaneously with IGF 1 or preincubated with the cells for 48 h prior to the addition of IGF 1 . ^^^ By contrast , maximum effective concentrations of IGFBP 3 ( 52 nM ) potentiated the effect of IGF 1 50 200 % when preincubated with bovine fibroblasts for 48 h prior to the addition of IGF 1 . ^^^ These data suggest that IGFBP 3 production is developmentally regulated in human placental fibroblasts and inhibits their mitogenic response to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , insulin and C peptide were measured by radioimmunoassay . ^^^ There was a close correlation between IGFBP 3 levels and the sum of IGF 1 and IGF 2 levels before surgery ( r = 0 . 9 , P < 0 . 01 ) and this was maintained throughout the post operative period ( mean correlation coefficient of 0 . 86 + / 0 . 02 , P < 0 . 05 ) . ^^^ On days 2 and 3 there was a small but significant increase in the ratio between serum IGF 1 and IGFBP 3 levels compared with the preop ratio ( P < 0 . 05 and < 0 . 005 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH , IGF 1 , and IGFBP 3 levels were now similar in the two groups ; IGFBP 1 levels decreased in the cirrhotic patients but remained fivefold higher than the comparison value ( 10 . 6 + / 3 . 7 vs . 2 . 1 + / 0 . 4 , P < . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have studied steady state levels of mRNA for the genes in the IGF 1 axis in normal and cirrhotic human liver , localised the most highly expressed gene , IGFBP 1 , and measured circulating IGFBP 3 by radioimmunoassay ( RIA ) , IGFBP 2 and IGFBP 3 by Western ligand blot ( WLB ) , and protease activity for IGFBP 3 in cirrhotic patients . ^^^ Messenger RNA for IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 was detectable by Northern blotting in normal and cirrhotic liver although there was considerable variation in expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The binding activity was inhibited by unlabelled IGFBP 3 at high doses ( 10 micrograms / ml ) and reduced but not abolished by preincubation of 125I IGFBP 3 with excess IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 in plasma of GH deficient rats . ^^^ The majority of IGF 1 circulates in a large ( 150 kDa ) ternary complex with IGF binding protein 3 ( IGFBP 3 ) and a non IGF binding acid labile subunit . ^^^ This study provides the first evidence in nondiabetic animals that insulin regulates hepatic IGF 1 mRNA expression , plasma IGF 1 and plasma IGFBP 3 levels in the GH deficient state without changes in hepatic GH receptors . ^^^ The divergent response of plasma IGF 1 and IGFBP 3 levels to insulin treatment in the present study may indicate an effect of insulin on the clearance of IGF 1 from the circulation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At our clinic we follow the development of MSA of specific binding proteins ( BP ) , trying to introduce or modify the described techniques f . e . the analyses of BP of GH ( GHBP ) , BP for IGF 1 ( IGFBP 3 , IGFBP 1 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Regulation of endothelial IGFBP 3 synthesis and secretion by IGF 1 and TGF beta . ^^^ We have examined the regulation of endothelial IGFBP 3 production by IGF 1 and TGF beta , two growth factors thought to play a major roles in the complications of diabetes mellitus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ The addition of 0 . 1 mol / l NaCl to 50 mmol / l phosphate buffer increased the affinity of IGF 2 for IGFBP 3 by 2 fold , and of IGF 1 by 4 fold . ^^^ Under ' physiological ' conditions , the affinities of ALS binding to IGF 1 IGFBP 3 and IGF 2 IGFBP 3 complexes ( 2 . 5 10 10 ( 8 ) l / mol and 5 . 8 10 10 ( 7 ) l / mol , respectively ) are 300 fold and 2000 fold lower than the constants for the formation of the corresponding binary complexes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , serum total IGF 1 and IGFBP 3 decreased dose dependently : total IGF 1 averaged 1470 + / 100 micrograms / l ( controls ; mean + / SEM ) , 1030 + / 60 micrograms / l ( low dose group ; P < 0 . 005 ) and 760 + / 40 micrograms / l ( high dose group ; P < 0 . 005 ) , whereas differences between IGFBP 3 levels reached significance ( P < 0 . 05 ) between controls and high dose rats , only . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated height velocity , bone age , urinary GH , serum IGF 1 and IGFBP 3 levels throughout the study ; plasma GHBP levels were determined only in the first 12 months of Gn RHa treatment . ^^^ Serum IGF 1 and IGFBP 3 levels decreased significantly at 12 months of Gn RHa therapy and increased significantly after Gn RHa plus hGH treatment . ^^^ During Gn RHa plus hGH therapy there was a significant increase in height velocity , in urinary GH levels , in serum IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 generation were correlated in all children ( delta SDS IGF 1 versus delta SDS IGFBP 3 , r = 0 . 49 , p = 0 . 03 ) . ^^^ Neither basal GHBP levels or the increment in GHBP were predictive of the IGF 1 or IGFBP 3 response to GH . ^^^ The GHI group had a significantly reduced IGFBP 3 response to stimulation with 4 d of GH ( median percent increment in IGFBP 3 , 26 % , versus 72 % in the GHD group , P = 0 . 03 ) ; their IGF 1 response to GH was also reduced ( median % increment in IGF 1 75 % versus 144 % in the GH deficient group ) , but this did not achieve significance , p = 0 . 06 . ^^^ In all children , the percentage rise or delta SDS in both IGF 1 and IGFBP 3 inversely correlated with the GH peak obtained on provocation testing , the latter being the most significant determinant of GH peak . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Radioimmunoassays were used to determine serum concentrations of IGF 1 and its binding protein , ( IGFBP 3 ) . ^^^ No significant differences were observed among any of the 3 patient groups and control subjects in the mean concentration of either IGF 1 or IGFBP 3 . ^^^ Likewise , the proportion of subjects with values above or below the laboratory ' s reference range did not differ for IGF 1 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In support of the first possibility , IGF binding protein 3 significantly inhibits the proliferation of CSF 1 treated bone marrow cells and this inhibition can be reversed by addition of exogenous IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased IGF BP 3 gene expression was only observed in the overlying cortex at 10 days post lesion , and corresponded with an increase in 125I IGF 1 binding at the injured surface of the cortex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , and IGFBP 3 were studied in the rhesus ( Macaca mulatta ) and long tailed ( Macaca fascicularis ) monkey fetus and dam during the second and third trimesters of pregnancy . ^^^ Thus , although IGF 1 , IGF 2 , and IGFBP 3 remained relatively unaffected in maternal sera during this period of gestation , fetal concentrations of IGF 1 , IGF 2 , and IGFBP 3 increased in a developmental profile similar to the human fetus . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal GH was 3 . 8 + / 0 . 7 ng / ml , insulin like growth factor 1 ( IGF 1 ) was 225 + / 38 ng / ml and IGF binding protein 3 was 3 . 0 + / 0 . 2 mg / l in 16 children studied with 10 linked hypophosphatemia . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Scatchard analysis of the data indicated a 20 fold loss of affinity for IGF 2 and an 50 fold loss for IGF 1 compared with that of recombinant human IGFBP 3 . ^^^ In a chick embryo fibroblast assay in which DNA synthesis was stimulated both by IGF 1 and by insulin ( at 100 fold concentrations , so that interaction with the Type 1 IGF receptor would occur ) , IGFBP 3 was found to inhibit IGF 1 induced stimulation almost totally . ^^^ Unexpectedly , with the fraction containing the 16 kDa fragment ( despite the total lack of affinity for IGF 1 ) , IGF 1 induced stimulation was inhibited to nearly the same extent as with intact IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , itself , had little effect on the production of IGFBPs , but when added together with bFGF increased the levels of IGFBP 3 and IGFBP 4 by 12 . 4 + / 5 . 1 fold ( P < 0 . 05 ) and 27 . 4 + / 5 . 3 fold ( P < 0 . 02 ) , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concomitant addition of IGFBP 3 to the perfusate significantly reduced the IGF 1 stimulated PA activity in the preovulatory follicles at 4 , 6 , and 8 h after exposure to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : AIDS wasting is associated with a GH resistant state that results in low levels of serum IGF 1 , IGF 2 and IGFBP 3 , elevated levels of phosphorylated IGFBP 1 , and a reduced ability to form the IGFBP 3 ternary complex . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Circulating IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels both increase in puberty where growth velocity is high . ^^^ Furthermore , IGFBP 3 proteolysis regulates the bioavailability of IGF 1 . ^^^ However , the concentration of free IGF 1 and possible IGFBP 3 proteolytic activity in puberty has not previously been studied . ^^^ In addition , total serum IGF 1 , IGFBP 1 and IGFBP 3 levels as well as IGFBP 3 protease activity were determined . ^^^ RESULTS : Free ( dissociable and ultrafiltrated ) IGF 1 concentrations were significantly higher in pubertal boys than in prepubertal children and correlated significantly with the molar ratio between IGF 1 and IGFBP 3 ( r = 0 . 69 , P < 0 . 0001 and r = 0 . 54 , P = 0 . 0008 , respectively ) and inversely with IGFBP 1 ( r = 0 . 47 , P < 0 . 0001 and r = 0 . 43 , P = 0 . 0003 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and GHBP levels in serum samples of 36 patients with adult onset GH deficiency . ^^^ The GHBP levels were measured by FPLC size exclusion chromatography ; IGF 1 and IGFBP 3 levels were measured by RIA . ^^^ Serum GHBP levels were elevated above the upper limit of the 95 % confidence interval in 26 patients , whereas IGF 1 and IGFBP 3 levels were low in 10 patients and in 16 patients , respectively . ^^^ The combination of low serum IGF 1 and low IGFBP 3 levels was found in 10 patients . ^^^ Low serum IGF 1 , low IGFBP 3 and elevated GHBP levels were found in five patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , a significant reduction in tumour size was accompanied by normalization of mean 24 h in GH insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Even intensified subcutaneous insulin therapy does not normalize GH , IGF 1 , GHBP and IGFBP 3 plasma levels . ^^^ Intraperitoneal insulin therapy administered to Type 1 diabetic patients by implantable pumps ( without modification of glycaemic control ) can improve GHBP activity , practically normalize plasma IGF 1 and normalize IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At the end of each period , plasma levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were measured at 0745 h , and 24 h profiles of plasma GH and cortisol were obtained at 15 min intervals together with the 24 h urinary excretion of free cortisol . ^^^ Plasma IGF 1 levels were increased in a dose dependent manner , whereas IGFBP 3 levels were increased only with the highest dosage . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To compare the effects of oral vs . transdermal estrogens on GH secretion and levels of circulating insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in younger vs . older postmenopausal women , we conducted a placebo controlled , cross over trial of 6 weeks of oral conjugated estrogen ( 1 . 25 mg daily ) or transdermal estradiol ( 100 micrograms / day ) administered in random order and separated by an 8 week , treatment free interval . ^^^ Spontaneous GH secretion , as assessed by 12 h overnight blood sampling at 20 min intervals ; GH responsiveness to i . v . bolus injection of GHRH ; and levels of serum IGF 1 and IGFBP 3 , before and after GHRH stimulation , were measured at enrollment and after 6 weeks of each estrogen treatment . ^^^ Before estrogen treatment , spontaneous nocturnal GH secretion and morning IGF 1 levels tended to be lower , IGFBP 3 levels did not differ , and GHRH stimulated GH levels were significantly reduced in older vs . younger postmenopausal women . ^^^ Oral estrogens increased spontaneous GH secretion , decreased serum IGF 1 levels , and did not alter IGFBP 3 levels , whereas transdermal estrogens did not alter nocturnal GH secretion or morning IGF 1 levels and decreased IGFBP 3 levels only in the older women . ^^^ In contrast , after , GHRH administration , IGF 1 levels were decreased only with oral estrogens , whereas IGFBP 3 levels were decreased with both oral ( younger women only ) and transdermal ( younger and older women ) estrogens . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Remarkable differences in the somatotropic axis were also observed ; although 24 h GH pulse frequency and levels of IGF 1 and IGFBP 3 were unaltered by either PCOS or obesity , the 24 h mean GH pulse amplitude was increased by 30 % ( P < 0 . 01 ) in lean PCOS in the presence of normal levels of high affinity GHBP and normal GH response to GHRH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 proteolysis was negatively correlated with IGF 1 ( r = 0 . 78 ) and IGFBP 3 levels ( r = 0 . 70 ) . ^^^ These results indicate that low levels of IGF 1 , IGFBP 3 , and acid labile subunit are associated with a failure to thrive in HIV infected children . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH and IGF binding protein 3 levels did not change ; however , IGF 1 levels were higher in the treatment group at 6 and 12 weeks . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have described the changes over time in WF IGF 1 concentrations , characterized the IGFBP profile , and demonstrated the presence of IGFBP 3 proteolytic activity in WF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH and IGF 1 exerted distinct effects on the relative weights of liver , kidney , and spleen and on the circulating levels of IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These include GH stimulation tests , 24 hour GH profiles , IGF 1 , and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ High quality determinations of IGF 1 and IGFBP 3 can be used to evaluate the GH IGF axis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH caused a significant increase in IGF 1 ( P < 0 . 0001 ) , which was far greater than the insignificant increase in serum IGFBP 3 levels ( P = 0 . 16 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Chondrocytes from osteoarthritic cartilage have increased expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) and 5 , but not IGF 2 or IGFBP 4 . ^^^ In osteoarthritic chondrocytes , IGF 1 message was increased 3 . 5 fold , IGFBP 3 was increased 24 fold , and IGFBP 5 was increased 16 fold over normal chondrocytes . ^^^ The observation that IGFBP 3 and 5 expression and production are elevated in osteoarthritic cartilage suggests that they may be acting as a competitor for IGF 1 in osteoarthritic cartilage , thus reducing the anabolic stimulation of this tissue and contributing to the net loss of cartilage in this disease . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
On the initial evaluation , GH levels failed to rise in response to hypoglycemia , IGF 1 levels were low , IGF binding protein 3 ( IGFBP 3 ) levels were suppressed , and IGFBP 2 levels were elevated . ^^^ GH therapy resulted in a normalization of the levels of blood sugars , IGFBP 3 , ALS , IGFBP 2 , and IGF 1 , as well as the IGF / IGFBP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the current study we performed daily IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 measurements in 9 healthy regularly cycling volunteers throughout the menstrual cycle . ^^^ In addition , we investigated IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 levels in 13 samples of androgen dominant follicular fluid [ FFa androstenedione to estradiol ( AD : E 2 ) ratio , > 4 ] and 19 samples of estrogen dominant follicular fluid ( FFe ; AD : E 2 ratio , 4 ) obtained from 21 regularly cycling subjects and in 18 samples of fluid from luteinizing follicles obtained from patients undergoing in vitro fertilization ( IVF ) treatment ( FFivf ) . ^^^ IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 were measured using two site immunoradiometric assays . ^^^ No significant day to day differences were observed in IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 levels across the menstrual cycle . ^^^ In contrast , significantly lower IGFBP 1 levels were found in FFe compared to FFivf ( 79 ng / mL ; range , 57 234 ; P = 0 . 002 ) , whereas there was no significant difference between FFe and FFivfe IGF 1 , IGF 2 , or IGFBP 3 levels , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In order to investigate the IGF regulatory pathway in leukaemia serum levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in 28 leukaemic children . ^^^ Whereas serum levels of IGF 1 ( mean / range : 2 . 7 / 0 . 1 to 6 . 7 SDS ) , IGF 2 ( 3 . 6 SDS / 1 . 3 to 8 . 7 ) and IGFBP 3 ( 2 . 0 / +2 . 2 to 7 . 1 SDS ) were significantly decreased comparable to levels in growth hormone deficiency , IGFBP 2 levels ( +4 . 0 / 0 . 45 to +7 . 4 SDS ) were found to be markedly elevated and inversely correlated to IGF 1 ( r = 0 . 51 , P = 0 . 013 ) . ^^^ CONCLUSION : This study demonstrates that the proliferation of malignant lymphoblasts ( at diagnosis vs treatment ) occurs in the presence of decreased serum levels of IGF 1 , IGF 2 and IGFBP 3 and that diminished production of these peptides may contribute to impaired growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin dependent diabetes mellitus is associated with decreased levels of circulating insulin like growth factor binding protein 3 ( IGFBP 3 ) , which are restored toward normal by treatment with insulin and / or infusion of insulin like growth factor 1 ( IGF 1 ) . ^^^ Addition of IGF 1 at 2 . 6 10 10 ( 8 ) M and 5 . 3 10 10 ( 8 ) M increased IGFBP 3 release by 5 . 2 and 8 . 2 fold ( both P < 0 . 05 vs . no IGF 1 ) , with concomitant increase in IGFBP 3 mRNA expression by 14 and 29 fold ( both P < 0 . 05 vs . no IGF 1 ) , suggesting regulation at a pretranslational level . ^^^ Further studies showed that IGF 1 did not have a significant effect on transcription initiation rates but prolonged the apparent half life of IGFBP 3 mRNA about 2 fold . ^^^ Stimulation of IGFBP 3 via type 1 IGF 1 receptors was evaluated by studies with [ QAYL ] IGF 1 ; the analog increased IGFBP 3 mRNA expression 220 + / 27 % above the level obtained without IGF 1 ( vs . 133 + / 9 % with wild type IGF 1 , P < 0 . 05 ) , suggesting involvement of receptor mediated synthesis . ^^^ CONCLUSION : Insulin stimulates IGFBP 3 gene transcription but provides proportionally greater increases in IGFBP 3 release , consistent with regulation at both transcriptional and posttranslational levels ; in contrast , IGF 1 alters IGFBP 3 expression by decreasing IGFBP 3 mRNA degradation , consistent with regulation at pretranslational and posttranscriptional levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Northern blots showed readily detectable transcripts for IGF 1 ( 6 . 7 and 0 . 9 kb ) , IGFBP 2 ( 1 . 8 kb ) , IGFBP 3 ( 2 . 8 kb ) , IGFBP 4 ( 2 . 6 kb ) , and IGFBP 5 ( 6 . 0 kb ) , but not for IGFBP 6 . ^^^ Dot blot analyses for IGF 1 and IGFBP 3 mRNAs were performed on total RNA from small and large luteal cells ; blots were counter probed with 3 phosphoglyceraldehyde dehydrogenase ( p GAD ) cDNA to assess RNA quantity and quality . ^^^ We conclude that porcine CL express mRNAs for IGF 1 and IGFBP 2 , 3 , 4 , and 5 , and that while small luteal cells are the major sources of IGF 1 and IGFBP 3 and 5 , IGFBP 2 and 4 appear to be expressed to approximately the same extent in small and large luteal cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of sex and age on patterns of circulating somatotropin ( ST ) concentration and plasma IGF 1 , IGF 2 , insulin , and IGF binding protein 3 ( IGFBP 3 ) were studied in ram , wether , and ewe lambs ( n = 7 or 8 ) sampled at mean ages of 81 ( I 1 ) and 158 d ( I 2 ) . ^^^ The IGF 1 and IGFBP 3 concentrations were greater in rams than in ewes at both sampling times . ^^^ Sex and age significantly affected patterns of circulating ST and concentrations of IGF 1 and IGFBP 3 in prepubertal growing lambs , under conditions for which growth rates and composition were also sexually dimorphic . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The normal range for serum IGF 2 in women was 490 1056 micrograms / L , and IGF 2 levels were positively correlated with serum concentrations of insulin like growth factor binding protein 3 ( IGFBP 3 ) but not with IGF 1 levels . ^^^ Mean serum concentrations of IGF 2 were reduced below normal in a number of hypopituitary patients and children with short stature and IGF 2 concentrations in these subjects correlated positively with IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 54 children with end stage renal disease ( ESRD ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was a close correlation ( r = 0 . 726 ) between serum IGF 1 and IGFBP 3 . ^^^ GH treatment increased serum IGF 1 and IGF BP 3 levels in all the patients examined although the responses were partly influenced by the order of GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to examine changes in serum IGF 1 and its major binding protein IGFBP 3 after pharmacological arrest of puberty . ^^^ Serum IGF 1 was measured by immunoradiometric assay ( IRMA ) and IGFBP 3 by both IRMA and Western ligand blotting ( WLB ) . ^^^ CONCLUSIONS : The lack of suppression of both IGF 1 and IGFBP 3 , despite therapy which halts puberty and slows growth velocity , suggests that steroids may be involved in the induction of changes in the GH / IGF 1 axis but not in their subsequent maintenance . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have therefore measured IGF 2 , as well as IGF 1 , and IGFBP 3 , levels in a large cohort of subjects with IDDM and in age / sex matched controls . ^^^ MEASUREMENTS : Random blood samples were obtained from each subject for the measurement of IGF 2 , IGF 1 and IGFBP 3 levels . ^^^ Multiple regression analysis revealed significant correlations between IGF 2 and IGFBP 3 ( F = 20 . 1 , P = < 0 . 001 ) and reaffirmed previously observed associations for IGF 1 and IGFBP 3 . ^^^ The sum of IGF 1 and IGF 2 ( expressed as nmol / l ) correlated with IGFBP 3 ; r = 0 . 47 in controls and 0 . 60 in diabetics . ^^^ The binding of IGFBP 3 for both IGF 1 and IGF 2 is unaltered by insulin dependent diabetes mellitus . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGFBP 3 bone gla protein ( BGP ) , procollagen 3 ( PIIINP ) , PTH and vitamin D , and bone mineral density ( BMD ) at proximal ( Prox ) and ultradistal ( Dist ) sites of the radius were measured . ^^^ RESULTS : Before treatment , IGF 1 , IGFBP 3 , BGP and PIIINP levels , as well as both Prox and Dist BMD , were significantly lower than in controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The expression and synthesis of IGF 1 and IGFBP 3 have been shown to be regulated by hormones and nutrition . ^^^ DESIGN : We measured serum IGF 1 and IGFBP 3 levels in a group of Malaysian aborigine children from three jungle settlements ; Sinderut and Pos Lanai are known for iodine deficiency and endemic goitre , and Gombak is an iodine replete area with better socioeconomic status . ^^^ IGF 1 and IGFBP 3 were determined by radioimmunoassay , and T 4 and TSH by immunoradiometric assay . ^^^ IGF 1 and IGFBP 3 correlated significantly with the height SDS of the children , In both the 4 10 ( r = 0 . 400 , P = 0 . 0001 and r = 0 . 365 , P = 0 . 0001 respectively ) and 11 15 years age groups ( r = 0 . 324 , P = 0 . 002 and r = 0 . 533 , P = 0 . 0001 respectively ) . ^^^ CONCLUSIONS : Varying duration and degree of exposure to malnutrition and iodine deficiency resulted in different mean levels of T 4 , TSH , IGF 1 and IGFBP 3 in the three areas . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGFBP 1 and IGFBP 3 were measured before and during treatment with tamoxifen up to 19+ months in 34 post menopausal patients with advanced breast cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The normally grown group showed a progressive decline in serum GH levels toward term ( r = 0 . 42 , p = 0 . 0001 ) , whereas serum IGF 1 was increased ( r = 0 . 55 , p = 0 . 0001 ) , as were serum IGF 2 ( r = 0 . 21 , p = 0 . 008 ) and IGFBP 3 levels ( r = 0 . 19 , p = 0 . 02 ) , although less markedly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At birth , when analyzed according to gestational age , serum GH levels were increased ( p = 0 . 0001 ) and serum IGF 1 and IGFBP 3 levels were decreased ( p = 0 . 0001 ) in IUGR as compared with normal neonates . ^^^ When two cohorts were established at birth as a function of the ponderal index ( PI ) ( < or = or > 3rd percentile ) , serum IGF 1 and IGFBP 3 levels were found to be significantly reduced in the case of low PI . ^^^ In conclusion , low serum IGF 1 and IGFBP 3 levels at birth were related to fetal malnutrition and were not predictive parameters for later growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ten children with cerebral palsy ( CP ) and growth failure underwent assessment of the growth hormone ( GH ) axis , including spontaneous GH secretion , GH secretion in response to pharmacological stimulation , and circulating levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : There was a significant increase in synovial fluid levels of both IGF 1 and IGFBP 3 in both RA and OA . ^^^ There were significant correlations between the CRP level and levels of IGF 1 , IGF 2 , and IGFBP 3 in the RA patients ( r = 0 . 62 0 . 898 , P = 0 . 04 0 . 0007 ) . ^^^ Levels of IGF 1 IGF 2 , and IGFBP 3 all correlated with the CRP level in patients with RA , which indicates the possibility that the IGF system is involved in the disease process . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the circulation , most of the IGFs are bound to a high molecular weight binding protein complex of 150 kDa that consists of IGF 1 ( or IGF 2 ) , IGFBP 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Periconceptual undernutrition resets plasma IGFBP levels and alters the response of IGFBP 1 , IGFBP 3 and IGF 1 to subsequent maternal undernutrition in fetal sheep . ^^^ Levels of IGFBP 1 were increased and IGFBP 3 , IGFBP 4 , IGF 1 , glucose and insulin were reduced at day 115 after undernutrition . ^^^ The degree of change in IGFBP 1 , IGFBP 3 and IGF 1 between day 105 and day 115 was greater in fetuses receiving low periconceptual nutrition . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 [ 1 88 ] , [ 1 184 ] , and [ delta 89 184 ] , none bound IGF 1 tracer by ligand blotting , although all were detectable by immunoblotting . ^^^ Normal sequence IGFBP 3 associated with the CHO cells and was partly displaceable by IGF 1 . ^^^ Whereas IGFBP 3 [ 1 88 ] and [ 1 184 ] failed to cell associate , the non IGF binding central deletion variant [ delta 89 184 ] did associate with CHO cells but was not displaced by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Adult rat serum contains two types of 150 kDa IGFBP complexes : ternary complexes containing bound IGF 1 , intact IGFBP 3 and the acid labile subunit ( ALS ) , and binary complexes that contain ALS and proteolytically nicked IGFBP 3 but which lack bound IGF . ^^^ We present evidence that the binary complexes containing proteolytically nicked IGFBP 3 can be formed in two ways : by direct association of IGFBP 3 with ALS in the absence of IGF , and by proteolysis of IGFBP 3 within 150 kDa ternary complexes , resulting in increased dissociation of IGF 1 . ^^^ Proteolysis of IGFBP 3 in the 150 kDa ternary complex provides a regulatable mechanism by which IGF 1 may be mobilized from the circulating reservoir of 150 kDa complexes to the tissues . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 exists primarily as part of a ternary 150 kDa complex comprising equimolar amounts of IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) . ^^^ It is also known that in contrast to IGF 1 and IGFBP 3 there exists a substantial quantity of unbound ALS in the circulation . ^^^ As part of our preclinical development program , we have investigated the pharmacokinetic properties of a complex of human recombinant IGF 1 and IGFBP 3 . ^^^ In contrast to the administration of free IGF 1 , IGF I / IGFBP 3 dosing leads to increased systemic IGF 1 exposure ( i . e . increased area under the time vs . serum concentration curve or AUC ) and decreased clearance ( CL ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Co administration of IGF binding protein 3 differentially inhibits the IGF 1 induced total body and organ growth of Snell dwarf mice . ^^^ In mammals IGF 1 is part of a 150 kDa binding protein complex , which also contains a glycosylated acid labile protein ( ALS ) and a glycosylated acid stable IGF binding subunit IGFBP 3 . ^^^ Since co injection with IGFBP 3 only partially blocked the hypoglycemic response of free IGF 1 in hypophysectomized rats , we were interested in the growth stimulating activity of the IGFI IGFBP 3 complex in pituitary deficient mice compared to that obtained by IGF 1 alone . ^^^ Therefore , the effects of subcutaneously administered IGF 1 , IGFBP 3 and the IGF 1 IGFBP 3 complex on somatic growth and organ growth of pituitary deficient Snell dwarf mice were studied after 4 weeks of treatment . ^^^ However , the stimulating effects of IGF 1 alone on body length and weight , as well as on the weight of the kidneys , were fully neutralized by co injection with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Intravenous bolus administration of IGFs to 55 kg pigs depressed blood glucose with the potency greatest for analogues such as des ( 1 3 ) IGF 1 , R3IGF 1 and Long R3IGF 1 that showed the weakest binding to pig IGFBP 3 , a similar efficacy pattern to that reported in the rat . ^^^ Chronic subcutaneous administration of Long R3IGF 1 , however , reduced growth rates , led to a depression in food intake and lowered concentrations of IGF 1 , IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recent studies demonstrate that IGF 2 in FFe is higher than in FFa ' whereas IGF 1 , IGFBP 3 and IGFBP 1 levels do not differ , underscoring the importance of local IGF 2 production by the granulosa and the importance of IGFBP 4 and IGFBP 2 in regulation of IGF 2 action within the follicle during its developmental pathway as an E 2 or A dominant follicle . ^^^ In the androgen treated female to male transsexual ( TSX ) model for PCOS , IGF 1 , IGF 2 , IGFBP 3 and IGFBP 1 levels do not differ . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of GH are believed to be mediated via IGF 1 , however , we were unable to mimic the effects of GH with IGF 1 , IGF 2 or a combination of IGF 1 , IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Transgenic mice expressing rat IGF 1 and human IGFBP 3 specifically in the mammary gland tissue , were created using the whey acidic protein gene . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this review paper , three pieces of clinical information in childhood are presented : ( 1 ) IGFBP 3 may replace GH provocation tests in the diagnosis of GH deficiency ( GHD ) ; ( 2 ) IGFBP 3 levels are regulated by IGF 1 levels in a short period , and ( 3 ) ratio of free IGF 1 to total IGF 1 is high in serum of early infancy , similarly to serum of pregnancy , only partially owing to the presence of IGFBP 3 proteolytic activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This paper reviews actions of antiestrogens on IGF physiology , and discusses the potential significance of the recent observations that ( 1 ) effects of antiestrogens on the uterus are correlated with their effects on uterine IGF 1 and IGFBP 3 gene expression , and that ( 2 ) the potent antiestrogen and growth inhibitor ICI 182 , 780 induces autocrine production of IGFBP 3 by estrogen receptor positive breast cancer cells , while the growth stimulatory action of estradiol is associated with suppression of IGFBP 3 expression . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 , an abundant IGFBP that binds IGF 1 and IGF 2 with high affinity , can form soluble inhibitory complexes with the IGFs that prevent them from binding to IGF 1 receptors . ^^^ These results suggest that direct interaction of heparin or IGF 1 with IGFBP 3 inhibits its ability to bind to the surface of GM 10 fibroblasts and C 6 glioma cells . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neither insulin like growth factor 1 ( IGF 1 ) nor IGF binding protein 3 ( IGFBP 3 ) alone could be used as a marker of GH insufficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH induced a significant increase in IGF 1 and IGF BP 3 levels ; IGF 1 peaked after 6 months ( when growth velocity was optimal ) and IGF BP 3 peaked after 12 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The objective of this study was to measure serum IGF 1 , IGFBP 3 , copper and zinc levels and to analyze their relationship to perinatal growth . ^^^ Serum IGF 1 ( RIA after acid ethanol extraction ) , IGFBP 3 ( RIA ) , copper and zinc ( atomic absorption spectrophotometry ) levels were measured in cord blood ( n = 78 ) and in newborn children 1 ( n = 110 ) and 3 ( n = 42 ) weeks after birth . ^^^ However , IGF 1 and IGFBP 3 levels increased significantly ( IGF 1 : 13 . 2 + / 15 . 5 vs 34 . 5 + / 27 . 3 ng / mL , p < 0 . 01 ; IGFBP 3 : 364 . 3 + / 185 . 1 vs 634 . 1 + / 306 . 9 ng / mL , p < 0 . 01 ) . ^^^ A positive relationship was found between IGF 1 and IGFBP 3 . ^^^ We conclude that intrauterine over nutrition is related to higher IGF 1 levels , whereas prematurity and intrauterine growth retardation are associated with lower zinc , IGF 1 and IGFBP 3 levels and with a lack of increase in copper serum levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As it has been hypothesized that IGF binding proteins ( IGFBPs ) may have a role as autocrine / paracrine factors in regulating the local actions of the insulin like growth factors ( IGFs ) in the ovary , we studied the production of the IGFBPs by human granulosa cells ( GC ) in culture and the role of IGFBP 3 in the modulation of ovarian cell responsiveness to IGF 1 and FSH . ^^^ Preincubation for 2 h at 22 C with IGFBP 3 , to allow the formation of the IGF IGFBP complex , drastically reduced the stimulatory effect of IGF 1 but not that of des ( 1 3 ) IGF 1 . ^^^ These data show that : 1 ) the IGF peptide is less active when bound to IGFBP 3 ; 2 ) as IGFBP 3 does not affect the potency of des ( 1 3 ) IGF 1 , its inhibitory action is exerted upstream of the membrane receptor binding ; 3 ) as the action of IGFBP 3 is exerted by binding the IGF peptide , its inhibitory effect on FSH points out the role of the locally produced IGF 2 in potentiating the FSH action on human GC . . ^^^ To this purpose , human luteinizing GC were cultured in serum free conditions for 24 h and subsequently submitted to increasing concentrations ( 2 8 nmol / l ) of recombinant non glycosylated or partially glycosylated IGF BP 3 for 48 h , in the presence or absence of IGF 1 , des ( 1 3 ) IGF 1 a truncated analog of human IGF 1 with markedly reduced binding ability to IGFBPs and FSH ( 5 20 mIU / ml ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were assayed by RIA for GH , IGF 1 and 2 , IGF binding protein 3 ( IGFBP 3 ) , and IGFBP 3 proteolytic activity . ^^^ After 10 min of low intensity exercise , IGF 1 and IGFBP 3 had increased over preexercise baseline by 7 . 7 + / 2 . 7 % ( P < 0 . 05 ) and 12 . 5 + / 3 . 3 % ( P < 0 . 004 ) , respectively . ^^^ After 10 min of high intensity exercise , all measured components of the IGF system were increased : IGF 1 by 13 . 3 + / 3 . 2 % ( P < 0 . 002 ) , IGF 2 by 15 . 7 + / 3 . 1 ( P < 0 . 01 ) , and IGFBP 3 by 23 + / 6 % ( P < 0 . 001 ) . ^^^ In summary : 1 ) brief exercise leads to small but significant increases in circulating IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 proteolysis ; and 2 ) these responses may be influenced by exercise intensity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Incubation of SMC cultures with IGF 1 and the IGF 1 analogs long R3IGF 1 and des ( 1 3 ) IGF 1 , which have decreased affinity for IGFBPs , revealed a facilitating effect of IGFBPs on the growth stimulating activity of a high concentration of IGF 1 in cell lines with high IGFBP 3 levels . ^^^ This suggests that a negative modulating role of IGFBP 3 due to sequestration of IGF 1 , as occurs in culture medium , is less relevant in vivo . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline serum concentrations of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were low ( 8 . 4 + / 2 ng / ml and 0 . 2 + / 0 . 1 mg / l respectively ) . ^^^ In the four children who completed the study , serum IGF 1 and IGFBP 3 levels did not change after treatment with rhGH ( 0 . 1 mg / kg / day ) for 4 days . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MATERIALS AND METHODS : Serum samples were collected from 60 normal full term neonates on day 5 and at 1 and 4 months of age to evaluate the GH concentrations by using both an immunofluorometric assay and Nb 2 cell bioassay , as well as the GH binding protein , IGF 1 , and IGF binding protein 3 values by radioimmunoassay . ^^^ In 5 day old neonates , we observed significantly lower ( P < . 001 ) serum GH binding protein ( 9 . 73 % + / 0 . 42 % ) , IGF 1 ( 67 . 63 + / 5 . 20 ng / mL ) , and IGF binding protein 3 ( 1 . 46 + / 0 . 17 mg / L ) concentrations compared with those in the prepubertal children ( 30 . 74 % + / 2 . 01 % , 210 + / 25 ng / mL , and 3 . 08 + / 0 . 22 mg / L , respectively ) . ^^^ At 1 month of age , serum GH binding protein ( 16 . 00 % + / 0 . 70 % ) and IGF binding protein 3 ( 2 . 96 + / 0 . 30 mg / L ) values were increased significantly ( P < . 001 ) , while IGF 1 levels ( 72 . 55 + / 7 . 6 ng / mL , P = . 09 ) were not increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To evaluate whether treatment with topical budesonide has any adverse effects on growth hormone dependent serum levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) , and on the serum markers of bone and collagen turnover osteocalcin , the carboxy terminal propeptide of type 1 collagen ( PICP ) , the carboxy terminal pyridinoline cross linked telopeptide of type 1 collagen ( ICTP ) and the amino terminal propeptide of type 3 procollagen ( PIIINP ) . ^^^ No statistically significant effects on serum levels of IGF 1 , IGFBP 3 , osteocalcin or ICTP were observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 , and IGFBPs 1 4 , were readily identified by specific immunoassays and / or 125iodine IGF ligand blotting of simultaneously obtained PD and serum samples from seven CRF children ; IGFBP 3 was a major IGFBP in PD as in serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , radioimmuno assays for insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) and IGFBP 1 were performed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and a range of growth and nutritional variables were investigated in 62 infants with congenital heart disease and healthy controls . ^^^ Serum IGF 1 and IGFBP 3 concentrations were significantly reduced . ^^^ Decreased IGF 1 and IGFBP 3 levels are observed in nutritional deficiency ; similar findings in congenital heart disease suggest that undernutrition contributes to the poor growth of these infants . ^^^ Serial measurements of serum IGF 1 and IGFBP 3 may be helpful in monitoring the effect of nutritional treatment in congenital heart disease . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , we have measured PSA , IGF 1 , IGF 2 , IGFBP 1 and IGFBP 3 in tumour tissue cytosols from 200 women with primary breast cancer , and have examined relationships between IGFs or IGFBPs and PSA along with other markers , including p 53 protein , steroid hormone receptors ( oestrogen and progesterone ) , cathepsin D , epidermal growth factor receptor , Her 2 / neu protein , S phase fraction and DNA ploidy . ^^^ Correlations or associations between PSA and IGF 1 , IGF 2 , IGFBP 1 or IGFBP 3 were not observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The circulating insulin like growth factors ( IGF 1 and 2 ) occur largely as components of a 140 kDa protein complex with IGF binding protein 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured levels of maternal and cord serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 in 8 White Class B insulin dependent DP and 8 non diabetic pregnancies ( NP ) . ^^^ No significant differences were found between DP and NP with respect to cord serum IGF 2 , maternal serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 , and the concentration and affinity of TR for IN , IGF 1 and IGF 2 . ^^^ Analysis of variance revealed an interaction between infant weight and the weight of the placenta ( p < 0 . 01 ) , cord IGF 1 ( p < 0 . 02 ) , cord C peptide ( p < 0 . 01 ) and cord IGFBP 3 ( p < 0 . 01 ) . ^^^ Regression analysis revealed significant correlations of cord IGF 1 with cord values of IGFBP 2 ( r = 0 . 52 , p = 0 . 04 ) and IGFBP 3 ( r = 0 . 66 , p < 0 . 005 ) . ^^^ Maternal serum IGF 1 significantly correlated only with maternal IGFBP 3 ( r = 0 . 65 , p < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Aims of the study were to assess the relationship between serum levels of IGFBP 3 with IGF 1 serum levels , peak GH levels in response to two stimulation tests ( PKGH ) and GH urinary excretion in children , and to examine the usefulness of IGFBP 3 for diagnosis of GH deficiency . ^^^ In all children , serum levels of IGF 1 and IGFBP 3 were measured and GH urinary excretion was assessed in overnight urine collections . ^^^ IGFBP 3 SDS was strongly correlated with IGF 1 SDS ( r = 0 . 63 , p < 0 . 001 ) , significantly correlated with peak GH ( r = 0 . 42 , p < 0 . 05 ) and weakly , but not significantly , correlated with urinary GH excretion . ^^^ In conclusion , IGFBP 3 levels are strongly correlated with IGF 1 levels , weakly correlated with peak GH response to provocative tests and may serve as a valuable parameter , in combination with others , in the evaluation of the GH IGF axis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Short stature and low IGF 1 and IGFBP 3 despite normal growth hormone secretion in a 4 year old girl with primary empty sella syndrome . ^^^ IGF 1 and IGFBP 3 were at and below the 1st percentile , respectively . ^^^ GH therapy led to an increase of growth velocity from 4 . 5 to 10 . 8 cm / year and a normalization of IGF 1 and IGFBP 3 levels . ^^^ The data indicate that measurements of IGF 1 and IGFBP 3 may be a more sensitive test for integrative GH activity than GH testing itself . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , dietary manipulation causes profound alterations in the IGF binding proteins , including reduced IGFBP 3 , the principal serum carrier of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The relative abundance of growth hormone receptor ( GHR ) , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) mRNA was assessed in mammary gland , maternal liver and heart , and in fetal and placental tissues . ^^^ There was no detectable change in mammary tissue GHR , IGF 1 or IGFBP 3 gene expression with either bGH or oPL treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , insulin and IGFBP 1 were measured by specific RIAs , and IGFBP 2 and IGFBP 3 were quantified by densitometry after Western ligand blotting . ^^^ The ratio between IGF 1 and IGFBP 2 or IGFBP 3 did not change after dialysis . ^^^ In ESRD patients predialysis mean values of insulin , IGF 1 SD score , IGFBP 1 and IGFBP 2 were increased , while the mean densitrometric values of the IGFBP 3 bands on Western ligand blot were either normal or reduced . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Physiologically , IGFBP 3 undergoes limited proteolysis which results in facilitated dissociation of the IGFs , particularly IGF 1 , and an increase in their turnover . ^^^ These results suggest that growth retardation in short children involves IGF 1 deficiency resulting from both decreased IGF 1 synthesis and lesser bioavailability of the circulating IGF 1 bound to IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The normal values of insulin like growth factor 1 ( IGF 1 ) , IGF binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) , and the high affinity growth hormone binding protein ( GHBP ) are not well established in large series of healthy fullterm newborns . ^^^ We report the normative data for IGF 1 , IGFBP 1 , IGFBP 3 , and GHBP in 271 normal Spanish full term newborns , born between 37 and 42 weeks of gestation , and compare these results with the same parameters studied in 39 premature infants . ^^^ Serum GHBP , IGF 1 , and IGFBP 3 levels are very low in the premature infant and show a significant increase in full term newborns , and continue to decline during childhood ( p < 0 . 001 ; analysis of variance ) . ^^^ A positive correlation between GHBP , IGF 1 , and IGFBP 3 versus gestational age was observed . ^^^ There is a direct relationship between the ponderal index and IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The pattern of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) concentrations could support this hypothesis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As previously described , there were significant decreases in circulating levels of IGF 1 and IGFBP 3 ( 38 42 kDa band ) , and an increase in the 30 kDa IGFBP ( likely corresponding to IGFBP 1 ) in poorly controlled diabetic animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
One aspect of this control is unquestioned : circulating GH acts on the liver to stimulate expression of the IGF 1 and IGFBP 3 genes , substantially increasing the levels of these proteins in the circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Cord sera were obtained from term , Chilean newborns exhibiting various patterns of intrauterine growth and assayed for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 by specific radioimmunoassays ( RIA ) . ^^^ In addition IGFBP 3 levels positively correlated to IGF 1 levels ( r = 0 . 614 , P = 0 . 0005 ) . ^^^ These data point to particularly important roles for IGF 1 and IGFBP 3 in regulating fetal growth at term , and suggest that they are regulated in a coordinated manner during the latter stage of gestation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Messenger RNA for GH receptor , IGF 1 , IGFBP 2 , IGFBP 3 , and actin were measured by nuclease protection assays . ^^^ The GH receptor mRNA was decreased in cows treated with GH whereas the mRNA for IGF 1 , IGFBP 2 , or IGFBP 3 was not changed . ^^^ In summary , the level of mRNA encoding GH receptor , IGF 1 , IGFBP 2 , and IGFBP 3 varied within the tissues examined , suggesting that these genes may play a variety of roles in the bovine female reproductive tract . ^^^ Supplemental GH failed to change the expression of IGF 1 , IGFBP 2 , and IGFBP 3 mRNA , possibly because of low GH receptor mRNA levels in tissues other than CL . ^^^ A direct action of GH on IGF 1 , IGFBP 2 , or IGFBP 3 gene expression within cow reproductive tissues was not supported because the amount of IGF 1 , IGFBP 2 , or IGFBP 3 mRNA was not altered by GH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were taken on days 15 , 29 , 43 , 55 , 90 , 120 , 150 , 180 for GH , IGF 1 , IGFBP 3 , IGFBP 1 and insulin measurements . ^^^ Serum IGFBP 3 levels were higher than normal and positively correlated with those of GH , IGF 1 and insulin . ^^^ During chronic octreotide treatment , positive correlations were found between GH and IGF 1 , GH and IGFBP 3 , IGF 1 and IGFBP 3 , insulin and IGFBP 3 and insulin and IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied puberty related changes in serum IGF 1 and IGFBP 3 levels in 24 patients ( 11 prepubertal ) with insulin dependent diabetes mellitus ( IDDM ) and 26 healthy subjects ( 14 prepubertal ) . ^^^ Serum IGF 1 and IGFBP 3 levels were assayed using immunoradiometric assays and radioimmunoassays , respectively . ^^^ Serum IGF 1 and IGFBP 3 levels in diabetics did not increase during puberty , as opposed to those in healthy children . ^^^ Serum IGF 1 and IGFBP 3 levels of diabetic patients were found to be lower than those of control subjects during puberty ( p < 0 . 0001 and p < 0 . 05 , respectively ) . ^^^ Our data displaying low IGFBP 3 levels in diabetic children may be due to increased proteolysis , which also causes a shift in IGF 1 to its lower molecular weight forms . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in growing preterm infants on enteral nutrition . ^^^ We analyzed the range of serum concentrations of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) and their inter relationships with age and some parameters of nutritional and hormonal status in 46 growing preterm infants on enteral nutrition . 72 nutritional balances were performed , with a cross sectional study design , at a mean age of 35 . 3 + / 17 . 2 days , equivalent to a mean corrected age ( gestational + postnatal age ) of 36 + / 2 . 3 weeks . ^^^ Serum concentrations of IGF 1 ( mean 64 + / 36 ng / ml ) and IGFBP 3 ( mean 1 . 15 + / 0 . 53 mg / l ) correlated significantly with each other ( r = 0 . 46 ) and both correlated with body weight ( r = 0 . 43 and 0 . 34 ) , body length ( r = 0 . 44 and 0 . 36 ) and serum concentrations of prealbumin , apolipoprotein A and cholesterol . ^^^ There was a weak correlation between IGFBP 3 and postnatal age ( r = 0 . 36 ) but no correlation between IGF 1 and IGFBP 3 and correlated age or urinary excretion of growth hormone . ^^^ In growing preterm infants , at least until 40 weeks of corrected age , serum concentrations of IGF 1 and IGFBP 3 seem to be related principally to body weight , body length and nutritional factors , but not to growth hormone . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 , IGF 1 , is believed to be an important anabolic modulator of cartilage metabolism and its bioactivity and bioavailability is regulated , in part , by IGF 1 binding protein 3 ( IGFBP 3 ) . ^^^ Prostaglandin E 2 ( PGE 2 ) stimulates IGF 1 production by articular chondrocytes and we determined whether the eicosanoid could regulate IGFBP 3 and , as such , act as a modifier of IGF 1 action at a different level . ^^^ Taken together , our results suggest that PGE 2 modulates IGFBP 3 expression , protein synthesis , and secretion , and that such regulation may modify human chondrocyte responsiveness to IGF 1 and influence cartilage metabolism . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma prorenin and renin , serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein ( IGFBP 2 and IGFBP 3 ) concentrations were measured in 22 randomly selected male and female patients with insulin dependent diabetes mellitus ( IDDM ) or non IDDM ( NIDDM ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also measured ultrafiltrated serum free IGF 1 and IGF 2 , total IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and insulin in a subgroup of 18 placebo treated and 20 lanreotide treated patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
HEX on the acute GH response to the hexapeptide and on serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , prolactin and cortisol levels was studied in seven elderly subjects ( four males and three females , aged 67 80 years ) . ^^^ On the other hand , HEX treatment did not significantly modify IGF 1 ( 138 . 7 + / 11 . 1 vs 122 . 4 + / 14 . 1 micrograms / l ) but increased IGFBP 3 levels ( 2 . 4 + / 0 . 2 vs 1 . 6 + / 0 . 2 mg / l , p < 0 . 02 ) . ^^^ Both IGF 1 and IGFBP 3 levels were slightly but significantly increased by oral HEX treatment ( 156 . 0 + / 10 . 7 vs 141 . 6 + / 13 . 6 micrograms / l , p < 0 . 03 , 3 . 4 + / 0 . 2 vs 3 . 1 + / 0 . 2 mg / l , p < 0 . 03 , respectively ) . ^^^ Moreover , after HEX treatment a trend towards increase was shown for IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes in serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels during growth hormone treatment in prepubertal short children born small for gestational age . ^^^ The aims of this study were to describe the basal serum levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) and to evaluate their changes during daily GH treatment ( 0 . 1 IU / kg ; 33 micrograms / kg ) for up to 2 yr in prepubertal short children born small for gestational age ( SGA ) and to correlate these changes with the growth response to GH therapy . ^^^ The serum concentrations of IGF 1 and IGFBP 3 were converted into SDS using our reference values for prepubertal healthy children . ^^^ Basal levels of both IGF 1 and IGFBP 3 were significantly reduced compared with the reference values . ^^^ The 1 yr growth response , expressed as the change in height SD score , correlated negatively with both the basal serum concentration of IGF 1 ( r = 0 . 37 ; P < 0 . 001 ) and IGFBP 3 ( r = 0 . 35 ; P < 0 . 01 ) , whereas a positive correlation was found to the 10 day percent increase in IGF 1 ( r = 0 . 32 ; P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Mean insulin like growth factor 1 ( IGF 1 ) and IGFBP 3 plasma levels increased significantly , without significant differences between groups . delta HSDSCA during GH was dependent on IGF 1 plasma levels at baseline and during the study period , beta 0 . 002 and beta 0 . 0004 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 were measured every 6 h . ^^^ IGF 1 and IGF binding protein 3 concentrations were not different among groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The restricted younger animals reduced serum IGF 1 IGF binding protein 3 , acid labile subunit , and growth hormone binding protein levels significantly more than the restricted older animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured the serum levels of these collagens by radioimmunoassay ( RIA ) , insulin like growth factor 1 ( IGF 1 ) ( by immunoradiometric assay ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) ( by RIA ) levels in 24 children aged between 4 and 14 years with chronic renal failure ( CRF ) ( n = 12 dialysis , n = 12 non dialysis ) and 12 age matched healthy controls , to find out whether these parameters have a prognostic or therapeutic value on monitoring of growth retardation in CRF . ^^^ It seemed that the pubertal stage of the patients did not affect the levels of PICP , PIIINP , and IGFBP 3 while serum IGF 1 levels in pubertal patients were significantly higher than those in prepubertal patients ( P < 0 . 001 ) . ^^^ Neither PICP nor PIIINP correlated with height z score , bone age , IGF 1 , or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In comparison with IGFBP 3 , rh mac 25 has at least a 5 6 fold lower affinity for IGF 1 and 20 25 fold lower affinity for IGF 2 . mac 25 mRNA was detectable in a wide range of normal human tissues , with decreased expression in breast , prostate , colon , and lung cancer cell lines . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 stimulated messenger RNA ( mRNA ) and media levels of IGFBP 3 . ^^^ In the human cells , IGF 1 increased media levels of IGFBP 3 through stimulation of IGFBP 3 mRNA and dissociation of cell bound IGFBP 3 , and decreased IGFBP 4 via potentiation of IGFBP 4 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recently IGF binding protein 3 ( IGFBP 3 ) serum concentrations have been reported to be a good marker of GH / IGF 1 axis activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Values of serum IGF 1 remained unchanged while plasma IGF binding protein 3 levels significantly decreased ( P < 0 . 05 ) during treatment and returned to pretreatment values after cessation of DHEA therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In seven subjects with IDDM , where serum IGFBP 1 and IGFBP 3 concentrations , and IGFBP 3 protease activity had also been measured in a previous study , the relationship between these variables and circulating free IGF 1 concentrations were examined by linear regression analysis . ^^^ There was no significant correlation between free IGF 1 and either serum IGFBP 3 concentrations or IGFBP 3 protease activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum profiles of GH , IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , insulin , glucose and non esterified fatty acids ( NEFA ) were recorded during the studies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The absence of IGFBPs other than IGFBP 3 in bovine preovulatory follicles may allow for increased availability of IGF 1 , which is proposed to be important for oocyte maturation and ovulation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It is proposed that the evaluation of short stature is best pursued by careful auxology plus assessment of the insulin like growth factor ( IGF ) axis , with particular attention to measurements of serum concentrations of IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Spontaneous circulating levels of hormones of the GH axis [ 24 hour integrated GH concentration , serum insulin like growth factor 1 ( IGF 1 ) or IGF binding protein 3 ] have been used in the diagnosis of childhood GH deficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although levels of IGF 1 and IGFBP 3 did not differ in either CA or AA , the 2 to 4 fold higher levels of IGFBP 1 in AA than in CA and CS resulted in a 3 fold reduced ratio of IGF I / IGFBP 1 in AA , which may decrease the bioactivity and hypoglycemic effect of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , we examined whether a short term fast ( approximately 12 h ) alters the serum concentration of free / dissociable IGF 1 , and whether these changes are associated with alterations in IGFBP 1 and the proteolysis status of IGFBP 3 . ^^^ There was no difference in IGFBP 3 and total acid ethanol extractable IGF 1 concentrations in serum from fasted and fed subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Genetic and environmental components of interindividual variation in circulating levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 . ^^^ We assessed the magnitude of the genetic component in the variation of circulating levels of insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) , and their binding proteins IGFBP 1 and IGFBP 3 by measuring their serum concentrations in 32 monozygotic and 47 dizygotic adult twin pairs of the same sex . ^^^ The proportion of variance attributable to genetic effects was 38 % for the IGF 1 concentration , 66 % for the IGF 2 concentration , and 60 % for the IGFBP 3 concentration . ^^^ Our results show that , in adults , there is a substantial genetic contribution responsible for interindividual variation of the circulating levels of IGF 1 , IGF 2 , and IGFBP 3 , but not for the IGFBP 1 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Steady state hepatic IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 mRNA levels were measured by Northern blot analysis using specific rat cDNA probes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The co ordinate maximum expression of mRNA for both IGF 1 and IGF 2 , the type 1 IGF receptor and IGFBP 3 during the period when the gametes and embryo are in transit suggests a role for IGF 1 and possibly IGF 2 peptides in providing an oviductal environment propitious to conception and early embryonic growth and metabolism . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Intraperitoneal insulin delivery , allowing primary portal venous absorption , may influence GH sensitivity , and improve hepatic IGF 1 and IGFBP 3 generation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Skeletal immaturity , IGF 1 and IGFBP 3 serum concentrations in Legg Calv Perthes disease ( skeletal immaturity , IGF 1 and IGFBP 3 in LCPD ) . ^^^ PATIENTS : In this study IGF 1 and IGFBP 3 were measured in the serum of 23 children with unilateral LCPD and in 23 sex and age matched controls . ^^^ METHODS : IGF 1 and IGFBP 3 were measured with radioimmunoassays , using an IGF binding site blocked assay for IGF 1 . ^^^ Chronological age and bone age related IGF 1 and IGFBP 3 serum concentrations were predominantly within the normal ranges and did not differ significantly from the matched controls . ^^^ IGF 1 and IGFBP 3 serum levels showed a high correlation , which was similar in LCPD ( r = 0 . 7 ; p < 0 . 0001 ) and in the control group ( r = 0 . 8 ; p < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the effects of the marathon run on the circulating levels of IGF 1 , IGFBP 1 , IGFBP 3 , and insulin in 23 participants . ^^^ The changes in IGF 1 and IGFBP 3 levels were less dramatic : the IGF 1 and IGFBP 3 levels were lower 1 and 3 days after the run . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To investigate the contribution of the insulin like growth factors ( IGFs ) and their binding proteins ( IGFBPs ) to the regulation of bone growth in 10 GH deficient Japanese children receiving recombinant GH therapy , we determined the percent increase from pretreatment levels of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , and bone specific alkaline phosphatase isoenzyme ( B ALP ) . ^^^ The elevation of IGF 1 induced by GH was significantly correlated with the increases in IGFBP 3 ( r = 0 . 735 ; P < 0 . 0001 ) and IGFBP 5 ( r = 0 . 795 ; P < 0 . 0001 ) , and the elevation of B ALP was also significantly positively correlated with the increases in IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 ( r = 0 . 544 , P < 0 . 0001 ; r = 0 . 268 , P = 0 . 0399 ; r = 0 . 414 , P = 0 . 0010 ; and r = 0 . 500 , P < 0 . 0001 , respectively ) . ^^^ Our data are consistent with the anabolic effect on bone growth of GH treatment being mediated by IGF 2 , IGFBP 3 , and IGFBP 5 as well as by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of treatment with the aromatase inhibitors aminoglutethimide ( AG ) and formestane or the synthetic progestin megestrol acetate ( MA ) on plasma levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding proteins ( IGFBPs ) , and IGFBP 3 protease status were investigated in 39 patients suffering from advanced breast cancer . ^^^ These alterations in plasma IGF 1 and IGFBP 3 protease activity were reversed 4 weeks after terminating MA therapy ( n = 8 ; P < 0 . 025 ) . ^^^ The increase in the plasma IGF 1 concentration seen during treatment with MA may be secondary to an increased level of intact IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of treatment with estrogens and antiandrogens in male to female ( M > F ) transsexuals and androgens in female to male ( F > M ) transsexuals on their respective bone metabolism , bone mineral density ( BMD ) , serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) levels were investigated . ^^^ Serum IGF 1 , IGFBP 3 , and propeptide of type 1 procollagen ( P1CP ) were measured at baseline and after 1 yr of treatment in 10 M > F and 10 F > M transsexuals . ^^^ Serum IGF 1 levels decreased significantly without significant changes in IGFBP 3 levels . ^^^ IGF 1 levels increased significantly , whereas no significant changes were seen in IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 3 levels were determined at 0 , 3 , 6 , 9 , and 12 months . ^^^ IGF 1 increased from 121 + / 13 to 240 + / 22 micrograms / L at 12 months ( P < 0 . 0001 ) , and IGF binding protein 3 increased from 2 . 65 + / 0 . 20 to 4 . 01 + / 0 . 42 mg / L at 12 months ( P = 0 . 0009 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Evaluation of IGF 1 and IGFBP 3 during diet therapy in patients with food allergy with complex nutritional risk ] . ^^^ The seric levels of IGF 1 and IGFBP 3 and their molar ratio , together with clinical and auxological parameters , have been evaluated in 16 patients ( 8 males and 8 females ) , of average age of 11 . 6 + / 8 . 66 months , who were suffering from complex food allergy and were subjected to a balanced diet will excluded the `` offending ' ' food . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : Insulin like growth factor 1 ( IGF 1 ) has been studied as a marker of nutrition in critical illness , but there is little research on IGF binding protein 3 , which regulates the bioactivity of IGF 1 . ^^^ A venous blood sample was collected at the time of admission into the study and was analyzed for serum IGF binding protein 3 concentration ( by radioimmunoassay ) , serum osmolality , IGF 1 concentration , and C reactive protein concentration . ^^^ Serum IGF 1 concentrations changed coordinately with IGF binding protein 3 concentrations in females and males ( r = . 62 , p < . 001 and r = . 54 , p < . 001 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : ( 1 ) Compared to 100 normal weight girls of same age the obese subjects had increased levels of IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 and normal levels of IGF 2 . ( 2 ) After weight loss ( 8 . 1 + / 2 . 0 kg ) IGF 2 and IGFBP 2 increased and IGF 1 , IGFBP 1 , IGFBP 3 and the ratio IGF I / IGFBP 3 decreased significantly . ( 3 ) Correlation analysis revealed that fasting insulin levels of the patients were positively correlated with IGF 1 but inversely with IGFBP 1 and IGFBP 2 . ^^^ The main conclusion is that obesity has to be considered for interpretation of serum concentrations of IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Use of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in the diagnosis of acromegaly and growth hormone deficiency in adults . ^^^ The goal of our study was to compare the clinical usefulness of plasma insulin like growth factor 1 ( IGF 1 ) ( with and without binding protein extraction ) and IGF binding protein 3 ( IGFBP 3 ) measurements in the diagnosis of growth hormone ( GH ) disorders in adults . ^^^ IGF 1 and IGFBP 3 concentrations were measured in 25 acromegalic and 25 GH deficient adult ( GHDA ) subjects ( 20 76 years ) by comparison to a control population ( n = 81 ) after age and sex stratification . ^^^ In untreated acromegaly , IGF 1 and IGFBP 3 were clearly increased ( 10 times the mean of controls for unextracted IGF 1 , 4 times for extracted IGF 1 and 2 times for IGFBP 3 ) . ^^^ Using the mean + 2SD of the control population as the cut off point , the sensitivity of IGF 1 for the diagnosis of acromegaly was higher than that of IGFBP 3 ( unextracted IGF 1 : 96 % and extracted IGF 1 : 100 % vs IGFBP 3 : 76 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Exogenous IGF 1 , IGF 2 , and an IGF 1 analog that binds poorly to IGF binding protein 3 ( des IGF 1 ) increased DNA synthesis of HL 60 and U 937 cells in a dose dependent ( 1 25 ng / ml ) fashion by 2 to 4 fold in serum free medium , whereas KG 1 cells did not respond to any of these growth factors . ^^^ The IGF induced increase in proliferation of HL 60 promyeloid cells was inhibited by soluble IGF binding protein 3 ( 500 ng / ml ) when these cells were stimulated with 10 ng / ml of either IGF 1 ( 53 + / 8 % ) or IGF 2 ( 59 + / 8 % ) , but not with des IGF 1 ( 3 + / 1 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Correlations between knemometric ( lower leg length ) growth rates and urine free cortisol excretion , respectively , and serum concentrations of IGF 1 , IGF binding protein 3 , osteocalcin , carboxy terminal propeptide of type 1 collagen ( PICP ) , carboxy terminal pryridinoline cross linked telopeptide of type 1 procollagen ( ICTP ) , and amino terminal propeptide of type 3 procollagen ( PIIINP ) were investigated in 17 asthmatic children aged 7 14 y during treatment with fluticasone propionate , 200 micrograms , and beclomethasone dipropionate , 400 and 800 micrograms / d , taken from dry powder inhalers . ^^^ Significant correlations ( Pearson ' s correlation coefficient , P ) between knemometric growth rates and IGF 1 ( 0 . 41 ; 0 . 006 ) , IGFBP 3 ( 0 . 35 ; 0 . 02 ) , PICP ( 0 . 44 ; 0 . 003 ) , ICTP ( 0 . 35 ; 0 . 001 ) , and PIIINP ( 0 . 46 ; 0 . 002 ) were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 and alpha 2 M in human seminal plasma were related to semen quality , basal concentrations of HGH , testosterone , IGFBP 3 , soluble fibronectin receptor ( sFNR ) , PSA and PP 14 in seminal plasma and to serum concentrations of luteinizing hormone ( LH ) and follicle stimulating hormone ( FSH ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 receptor , and five IGF binding proteins ( IGFBPs ) , IGFBP 1 , IGFBP 2 , IGFBP 3 , IGFBP 4 , and IGFBP 5 in smooth muscle cells ( SMCs ) using colloidal gold immunocytochemistry . ^^^ Quantitative reverse transcription polymerase chain reaction ( QRT PCR ) performed on de novo atherectomy specimens identified mRNA for IGF 1 , IGF 1 receptor , IGFBP 1 , IGFBP 2 , IGFBP 4 , IGFBP 5 levels and detected mRNA for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) levels were below normal but were lower in CO than AO patients ( P < 0 . 001 ) , and the correlation with IGF binding protein 3 was stronger in CO than in AO patients . ^^^ IGF 1 and IGF binding protein 3 were increased into the normal range by GH therapy in both groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the effect of 12 weeks of recombinant human GH therapy given in three different doses on serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in patients with GH deficiency ( GHD ) . ^^^ At the start of the study , we found lower levels of both serum IGF 1 and IGFBP 3 in childhood onset GHD than in adult onset GHD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In normal , nonpregnant plasma , most of the IGF 1 is complexed to specific IGF binding proteins ( IGFBPs ) , particularly IGFBP 3 ; only a minor fraction of plasma IGF 1 exists in a free form . ^^^ Recently , we have reported that free IGF 1 levels , as measured using a new immunoradiometric assay , are relatively high in maternal plasma during pregnancy because of increased IGFBP 3 proteolytic activity . ^^^ In the present study , we have analyzed free and total plasma IGF 1 and IGFBP 3 proteolytic activity in early infancy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , the acid labile subunit ( ALS ) , insulin , and IGFBP 1 were evaluated as indicators of body composition during GH replacement therapy in 20 GH deficient patients ( 9 women and 11 men ) , aged 22 57 yr , with IGF 1 levels below 2 SD . ^^^ Serum levels of IGF 1 , ALS , and IGFBP 3 above the normal range were reached in seven , five , and three subjects , respectively , after 12 months of GH therapy . ^^^ IGF 1 and ALS levels , but not IGFBP 3 levels , correlated with the total daily GH dose ( r = 0 . 676 ; P = 0 . 001 and r = 0 . 631 ; P = 0 . 003 ) . ^^^ At 12 months , the LBM values were significantly correlated to the IGF 1 levels ( r = 0 . 718 ; P < 0 . 001 ) , but not to ALS or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report maternal serum levels of IGF 1 , IGF 2 , and IGFBP 1 , 2 , and 3 ; IGFBP 1 phosphorylation ; and IGFBP 3 proteolysis in pregnancies ( n = 139 ) complicated by trisomy 18 or trisomy 21 compared with those in normal controls . ^^^ Maternal IGF 1 , IGF 2 , and IGFBP 3 showed no significant difference between fetuses with a normal karyotype and those with trisomy 18 or 21 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , we report that EGF treatment for 4 weeks in Goettingen minipigs reduces circulating IGF 1 and IGFBP 3 , increases circulating IGFBP 1 , IGFBP 2 and IGFBP 4 , and induces a slight hyperthyroidism as judged from increased circulating levels of T3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Metabolic acidosis significantly decreased serum IGF 1 concentration from 45 + / 6 to 33 + / 6 nmol / liter ( P = 0 . 002 ) , while serum IGF binding protein 3 concentration was not affected significantly . ^^^ Metabolic acidosis in humans results in a significant decrease in serum IGF 1 concentration without a demonstrable effect on IGF binding protein 3 , and is related to a resistance to the hepatocellular action of growth hormone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Hormone profiles ( IGF 1 , IGFBP 1 , IGFBP 3 , cortisol , insulin , glucagon , triiodothyronine [ T 3 ] , levothyroxine [ T 4 ] , and thyroxine binding globulin ) were taken . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We did not find any differences in serum IGF 1 , IGFBP 3 and urinary GH between CUG ( + ) and CUG ( ) infants at any time during the study . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured GH levels in blood samples obtained every 20 minutes from 8 : 00 PM to 8 : 00 AM ; AM serum levels of IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and GH binding protein ( GHBP ) ; muscle strength ; muscle histology ; the normalized phosphocreatine abundance , PCr / [ PCr + Pi ] , and intracellular pH in forearm muscle by NMRS during both sustained and ramped exercise ; body composition by dual energy 10 ray absorptiometry ( DEXA ) ; lipid levels ; and glucose , insulin , and GH levels during an oral glucose tolerance test ( OGTT ) . ^^^ GHRH treatment increased mean nocturnal GH release ( P < . 02 ) , the area under the GH peak ( [ AUPGH ] P < . 006 ) , and GH peak amplitude ( P < . 05 ) , with no change in GH pulse frequency or in levels of IGF 1 , IGFBP 3 , or GHBP Two of six measures of muscle strength , upright row ( P < . 02 ) and shoulder press ( P < . 04 ) , and a test of muscle endurance , abdominal crunch ( P < . 03 ) , improved . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 and IGFBP 3 concentrations were low during the fetal life and increased after birth . ^^^ In the kidney , IGFs , IGFBP 3 , IGFI R and IGFII R as well as GHR mRNA levels were relatively high during the fetal and early postnatal life . ^^^ In the skeletal muscle , IGFs , IGFBP 3 and IGFI R mRNA levels decreased with advancing age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although TAM can increase IGFBP 3 expression in MCF 7 cells , and this binding protein has been shown to be able to inhibit IGF action , TAM had no effect on IGF 1 stimulated tyrosine phosphorylation of IGF 1 receptor or the downstream signaling molecule , insulin receptor substrate 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study significantly higher concentrations of insulin like growth factor binding protein 3 ( IGFBP 3 ) , insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) and transforming growth factor beta 2 ( TGF beta 2 ) were found in the Na / K > 3 sub group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have examined , therefore , the performance of other tests of the GH axis [ urinary GH excretion , serum insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) levels ] compared with GH stimulation tests in identifying children defined clinically as GH deficient . ^^^ The sensitivities of IGF 1 , IGFBP 3 , and urinary GH , using a cut off of 2 SD score were poor at 34 % , 22 % , and 25 % , respectively , with specificities of 72 % , 92 % , and 76 % respectively . ^^^ We devised a scoring system based on the positive predictive value of each test , incorporating data from the GH test and the IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This report extends observations of rhIGF 1 efficacy at two dosage levels [ 120 micrograms / kg BW twice daily ( n = 15 ) and 80 micrograms / kg twice daily ( n = 7 ) ] over 2 yr , compares biochemical responses [ serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) ] and their relationship to growth effects , and compares treatment effects of rhIGF 1 in GHRD to rhGH in idiopathic GH deficiency ( GHD ) . ^^^ The growth response and favorable trough levels of IGF 1 despite the overall lack of increase in circulating IGFBP 3 levels suggest an alternative mechanism for sustaining IGF 1 levels and avoiding rapid clearance of rhIGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth of small bowel , abundance and localization of messenger RNAs for the IGF 1 transgene , and insulin like growth factor binding protein 3 were assayed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , oestradiol and norethisterone were analysed by radioimmuno assays . ^^^ Oral treatment caused a significant decrease ( 16 % , P < 0 . 005 ) in IGF 1 and a non significant decrease in IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : In JRA patients , IGF 1 , IGFBP 3 , and osteocalcin levels were significantly lower and insulin levels significantly higher than those in controls , but there was no significant difference in the level of IGFBP 1 . ^^^ Levels of IGFBP 3 correlated with those of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) reflect the secretion of endogenous growth hormone ( GH ) in healthy children and exhibit little diurnal variation , which makes them potential candidates for screening of GH deficiency ( GHD ) . ^^^ We evaluated serum IGF 1 and IGFBP 3 levels in relation to the outcome of GH provocative testing in 203 children and adolescents ( 111 boys and 92 girls ) in whom GHD was suspected . ^^^ Consequently the predictive value of a positive test result in prepubertal children was 88 . 8 % for IGF 1 and 90 % for IGFBP 3 . ^^^ Accordingly the positive predictive value and in 10 to 20 year old children was 52 . 3 % for IGF 1 and 56 . 5 % for IGFBP 3 . ^^^ Combination use of IGF 1 and IGFBP 3 gave additional diagnostic information . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
An IGF 1 domain has been identified that remains exposed after IGF 1 binding to IGFBP 1 or to IGFBP 3 , which is recognized by 6 different mAb . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal and growth hormone induced hepatic messenger ribonucleic acid expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 is independent of hyperinsulinemia and increased energy status in the genetically obese Zucker rat . ^^^ To further elucidate this point , we quantitated hepatic IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and GH receptor messenger RNAs ( mRNAs ) expression in obese Zucker rats under different serum GH and insulin conditions using lean rats as controls . ^^^ Under comparable serum GH levels in GH treated intact , hx , and GH treated hx animals , hyperinsulinemia and / or increased body weight present in obese rats were not associated with increased hepatic IGF 1 and IGFBP 3 mRNA amount . ^^^ These results suggest that in vivo the imbalance of the serum GH / IGF 1 axis present in obesity is primarily due to events distal to the hepatic IGF 1 and IGFBP 3 mRNAs expression , which is tightly correlated to GH levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The affinity of phosphorylated IGFBP 1 from plasma ( 2 . 3 + / 1 . 1 10 10 ( 10 ) M 1 ) also was significantly higher than the affinity of IGFBP 3 for IGF 1 ( 5 . 6 + / 4 . 2 10 10 ( 9 ) M 1 , P < 0 . 005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Arteriovenous differences were measured over working thigh for growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGF BP 3 ) , parathyroid hormone ( PTH ) and bone biomarkers , i . e . , the carboxyterminal propeptide of type 1 procollagen ( PICP ) , the carboxyterminal cross linked telopeptide of type 1 collagen ( ICTP ) , osteocalcin , and bone specific alkaline phosphatase ( b ALP ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We determined the circulating levels of IGF 1 and two of its key binding proteins , IGFBP 1 and IGFBP 3 . ^^^ The levels of IGFBP 1 in patients with PV ( 37 . 80 + / 4 . 33 microg / L ) were more than fourfold higher than in normals ( 9 . 34 + / 1 . 34 microg / L ) or patients with secondary erythrocytosis ( 9 . 47 + / 1 . 96 microg / L ) , whereas the plasma concentrations of IGFBP 3 and IGF 1 in these patients were similar to those of normal subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Whereas there seems to be little , if any , circadian variation in circulating concentrations of IGF 1 and IGFBP 3 in healthy subjects , there are conflicting reports on this issue in GH deficient patients treated with GH as a daily subcutaneous injection . ^^^ We have therefore investigated the 24 hour serum profiles of IGF 1 and IGFBP 3 concentrations after one week and more than one year of GH treatment . ^^^ The subjects were hospitalized for 24 hour blood sampling at 1 hour intervals and serum concentrations of GH , IGF 1 and IGFBP 3 were determined . ^^^ RESULTS : There was a significant diurnal variation in serum IGF 1 and IGFBP 3 concentrations both in the subjects who had received GH for one week and in those who had received GH treatment for more than one year . ^^^ The serum concentrations of IGF 1 and IGFBP 3 were highest in the morning and lowest during night time and early morning . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We determined the 24 hour integrated concentration of GH ( IC GH ) , GH response to provocative stimuli , IGF 1 , IGF binding protein 3 ( IGF BP 3 ) and GH binding protein ( GH BP ) in 34 prepubertal children ( 25 m / 9 f ) 5 10 years of age , height 2 . 5 to 1 . 5 SDS and body mass index 1 . 5 to 1 . 5 SDS for age and sex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Laboratory evaluation of growth included measurement of IGF 1 , IGFBP 3 , bone ages and GH response to pituitary provocative agents . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 levels fell while IGFBP 1 and 2 levels rose during treatment , changes that likely affect the bioavailability of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The decrease in IGFBP 3 and increase in lower molecular weight IGFBP may have contributed to the reduction in serum IGF 1 by increasing IGF 1 clearance from the circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSION : These results indicate a coordinate gene expression of the IGF system in microembolised porcine myocardium , compatible with a role of IGF 1 , IGFBP 3 , IGFBP 5 , and IGFBP 6 in inflammation linked angiogenesis and / or repair processes . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The hormonal regulation of the components of this complex is highly controversial , and both IGF 1 and GH have been shown to mediate the expression / synthesis of IGFBP 3 . ^^^ SKHEP 1 cells significantly increased the IGFBP 3 concentrations in conditioned medium ( CM ) when treated with GH ( 0 . 1 10 ng / ml ) , IGF 1 ( 1 100 ng / ml ) , or Des ( 1 3 ) IGF 1 ( 1 100 ng / ml ) in a dose dependent manner ( > 3 fold ) . ^^^ Interestingly , time course studies showed that the GH induced increase in IGFBP 3 mRNA preceded the IGF 1 induced increase ( 6 h for GH induced IGFBP 3 mRNA ; 12 h for IGF 1 induced IGFBP 3 mRNA ) . ^^^ The half life of IGFBP 3 mRNA was evaluated after transcriptional arrest by treatment with a RNA polymerase 2 inhibitor ( 5 , 6 dichloro 1beta D ribofuranosylbenzimidazole ) , and was found to be 14 18 h and unaltered by GH or IGF 1 treatment . ^^^ The induction of IGFBP 3 by GH was not due to the indirect action of locally synthesized IGF 1 , because 1 ) no immunoreactive IGF 1 was detected in the CM of control or GH treated cells ; 2 ) Northern blots revealed no IGF 1 mRNA expression in SKHEP 1 cells ; 3 ) reverse transcriptase PCR did not detect any expression of the IGF 1 gene ; and 4 ) time course studies showed an earlier increase in IGFBP 3 mRNA after GH treatment than after IGF 1 treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) concentration in 150 kilodalton complexes containing human IGF binding protein 3 ( hIGFBP 3 ) after intravenous injection of adult rats with hIGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 protease activity was inhibited in medium conditioned by cells incubated with 50 ng / ml IGF 1 . ^^^ The cell free inhibition of IGFBP 3 proteolysis by IGFs did not require IGF interaction with the binding protein , because [ long Arg 3 ] IGF 1 , which binds to IGFBP 3 with less than 0 . 2 % of the potency of IGF 1 , inhibited IGFBP 3 proteolysis with 20 % of the potency of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similarly to IGF 1 , IGFBP 3 is also internalized and accumulates in the endosomal compartment in resting cells whereas it is targeted to the nucleus in proliferating cells . ^^^ IGFBP 3 , which contains a putative nuclear targeting signal , may act as a carrier for IGF 1 nuclear transport . ^^^ The transport of IGF 1 and IGFBP 3 to two different compartments may influence their biological activity . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Clinical utility of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in the diagnosis of GH deficiency ( GHD ) during childhood . ^^^ Thus , three extreme subgroups ( G 1 ; normal , G 2 ; severe GHD , G 3 ; short children with normal GH secretion ) were selected in order to show that insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) levels reflect GH secretion . ^^^ IGF 1 and IGFBP 3 , together with free IGF 1 and acid labile subunit , may replace GH provocation tests in the diagnosis of GH deficiency during childhood ; these functional tests for GH secretion , which show minimal intradaily variation , are more physiological and cost effective than GH provocation test . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum Insulin like growth factor 2 ( IGF 2 ) levels in patients with acromegaly and growth hormone deficiency : correlation with IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Early follicular , late follicular , midluteal phase , and samples taken at ovulation were also assayed for IGF 1 and IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It could be shown that IGF 1 stimulated proliferation is inhibited by IGFBP 3 , which decreases the availability of IGFs for binding to the IGF receptors , and by beta galactosidase , which may decrease IGF binding to the type 2 IGF receptor , but is not inhibited by the anti type 1 IGF receptor monoclonal antibody alphaIR 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and DHEAS levels together with bone mineral density ( BMD ) of the femoral neck and lumbar spine in women with an autonomously functioning thyroid nodule . ^^^ Serum IGF 1 as well as IGFBP 3 were increased in the manifest hyperthyroid group , to 157 % ( P < 0 . 001 ) and 129 % ( P < 0 . 05 ) respectively , whereas DHEAS levels did not change in either premenopausal patient group . ^^^ In contrast to premenopausal women , serum IGF 1 and IGFBP 3 did not change in the two groups who were postmenopausal and serum DHEAS levels were reduced to 58 % ( P < 0 . 001 ) in both postmenopausal groups with subclinical as well as overt hyperthyroidism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No relationship was found between GHBP and spontaneous 24 h GH secretion , in terms of either GH secretion rate or pulsatile pattern , whereas GHBP was positively correlated with insulin like growth factor 1 ( IGF 1 ) SD score ( r = 0 . 28 ; P < 0 . 05 ) and IGF binding protein 3 SD score ( r = 0 . 39 ; P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGF 1 and IGF 2 ) and IGF binding protein 3 production by fibroblasts of patients with Turner ' s syndrome in culture . ^^^ Reports indicate that in plasma insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) are normal in patients with Turner ' s syndrome ( TS ) . ^^^ We compared the ability of fibroblasts from six TS patients with that of fibroblasts from six age matched control ( C ) subjects to synthesize in vitro IGF 1 , IGF 2 , and IGFBP 3 under basal and GH , estradiol ( E 2 ) , or GH plus E 2 stimulated conditions . ^^^ Furthermore , we evaluated IGF 1 , IGF 2 , and IGFBP 3 messenger ribonucleic acid ( mRNA ) expression in fibroblasts from TS and C subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study of GH , administered in double blind , placebo controlled , cross over fashion to regularly cycling women undergoing in vitro fertilization , we found that follicular fluid levels of IGFBP 1 , 3 , and 4 and serum levels of IGFBP 3 , as well as follicular fluid and serum IGF 1 , were significantly increased in the GH treated cycles , when compared with the placebo cycle of the same patient . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patients with insulin dependent diabetes mellitus ( IDDM ) exhibit abnormalities in the GH / insulin like growth factor ( IGF ) axis , including GH hypersecretion , low serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels , and elevated IGFBP 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone ( GH ) provocative retesting of 108 young adults with childhood onset GH deficiency and the diagnostic value of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 . ^^^ Serum levels of total insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) reflect the endogenous GH secretion in healthy children and exhibit little diurnal variation , which makes them good diagnostic markers for screening of GH deficiency ( GHD ) in short children , although some controversy still exists . ^^^ In adults , the diagnostic value of IGF 1 and IGFBP 3 suspected of GHD has been reported in only a few studies . ^^^ Basal IGF 1 and IGFBP 3 levels were compared to those in 1237 healthy controls ( 312 controls > 18 yr ) as well as to peak GH levels . ^^^ Multiple linear regression analysis demonstrated that IGF 1 and IGFBP 3 were significantly dependent on peak GH levels and the number of other pituitary axes affected . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum was assayed serially for glucose , IGF 1 , GH , and IGF binding protein 3 levels . ^^^ Serum GH was significantly elevated in groups C and D , whereas serum IGF 1 and IGFBP 3 were only significantly increased in group D . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentration of IGFBP 1 was negatively correlated ( r = 0 . 60 , P > 0 . 05 ) with insulin and IGFBP 3 showed a strong positive correlation with IGF 1 ( r = 0 . 90 , P > 0 . 01 ) . ^^^ Hyperinsulinaemia is associated with low circulating IGFBP 1 concentrations and IGFBP 3 seems to be an excellent indicator of the peripheral IGF 1 concentration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this assay , recombinant IGFBP 3 ( 5 ng ) was immobilized on plastic microtitre wells , after which radiolabelled IGF 1 or 2 was allowed to bind . ^^^ IGFBP 1 and IGFBP 3 , but not an N terminal fragment of IGFBP 3 , could effectively block binding of both IGF 1 and IGF 2 to the solid phase IGFBP 3 . ^^^ Our results with heparin also suggest that the binding sites on IGFBP 3 for IGF 1 and IGF 2 are not completely identical . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^ Serum IGF 2 but not IGF 1 or IGFBP 3 was higher in PC and BPH than in controls ( PC 332 + / 23 micrograms / l ) , BPH 359 + / 26 micrograms / l vs controls 241 + / 37 micrograms / l ; P = 0 . 03 and 0 . 02 respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : We analyzed the role that nutrition and the insulin like growth factors IGF 1 and IGFBP 3 play on neonatal growth . ^^^ Anthropmetric variables , daily intake of energy and nutrients , and serum levels of IGF 1 and IGFBP 3 were measured . ^^^ At the third week of postnatal life , serum IGF 1 and IGFBP 3 levels showed a significant increase . ^^^ Preterm infants born before 33 gestational weeks showed lower IGF 1 ( p < 0 . 02 ) and IGFBP 3 ( p < 0 . 02 ) levels than those born between 33 and 37 gestational weeks . ^^^ IGF 1 and IGFBP 3 were correlated between themselves and with energy and protein intake . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , GH binding protein ( GHBP ) , lipids , and safety laboratory tests ( complete blood count and chemistry profile ) were measured in fasting samples ( 0800 0900 h ) . ^^^ This was accompanied , within 2 weeks , by increased serum levels of IGF 1 ( P < 0 . 05 ) and IGFBP 3 ( P < 0 . 001 ) , but not IGFBP 1 , which remained elevated for 12 weeks , returning toward baseline by 16 weeks in both genders . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 levels were reduced among elders , but not to the same extent as IGF 1 , resulting in a relative excess of IGFBP 3 in elders ( IGFBP 3 / IGF 1 ratio , 20 . 1 + / 0 . 9 vs . 15 . 4 + / 1 . 0 ; P < 0 . 001 ) . ^^^ The IGFBP 3 / IGF 1 ratio correlated with IGF 1 levels in young and old men ( r = 0 . 79 ; P < 0 . 001 and r = 0 . 82 ; P < 0 . 001 ) , indicating that diminished GH secretion also may contribute to a relative excess of IGFBP 3 among elders . ^^^ Serum levels of IGFBP 1 ( but not IGF 1 or 2 or IGFBP 3 ) correlated with body mass index and upper arm fat and muscle areas in elders . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
While antioestrogens such as tamoxifen and droloxifene reduce the expression of IGF IR in vitro and suppress plasma levels of IGF 1 but elevate IGF binding protein 1 in vivo , megestrol acetate may reduce the delivery of IGFs to the tissues by inhibition of IGFBP 3 protease activity . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At the first evaluation , 2 . 9 + / 0 . 2 ( SE ) years after BMT , the values for the plasma insulin like growth factor 1 ( IGF 1 ) and its GH dependent binding protein ( IGFBP 3 ) were similar in groups 1 and 2 but significantly greater in group 3 ( p < 0 . 02 for IGF 1 and 0 . 01 for IGFBP 3 ) . ^^^ These values were similar in those patients in groups 1 and 2 who had low GH peaks after stimulation ( 12 patients : IGF 1 , 0 . 8 + / 0 . 2 U / ml ; IGFBP 3 , 1 . 6 + / 0 . 2 mg / L ) and in those with normal GH peaks ( 20 patients : 1 + / 0 . 1 U / ml and 1 . 8 + / 0 . 1 mg / L ) . ^^^ The individual height changes between BMT and the last clinical evaluation before GH therapy were not correlated with the age at BMT , GH peak after stimulation , plasma IGF 1 concentration , or IGFBP 3 concentration . ^^^ We conclude that plasma IGF 1 and IGFBP 3 values are of no diagnostic value for GH deficiency after TBI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Following a five day maternal fast , both IGF 1 and IGF 2 levels were decreased in the maternal and fetal circulation ( P < 0 . 05 ) , accompanied by a decrease in fetal hepatic IGF 1 mRNA levels , but the IGFBP 2 level was increased and the IGFBP 3 level was decreased in maternal circulation , whereas the IGFBP 1 level was increased in fetal circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENT : Total IGF 1 , free IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and insulin were measured in obese subjects and normal control subjects . ^^^ CONCLUSION : We confirmed that total IGF 1 and IGFBP 3 concentrations were not significantly different between the obese and control groups , despite GH hyposecretion in obesity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Comparative study of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) level and IGF I / IGFBP 3 ratio measurements and their relationship with an index of clinical activity in the management of patients with acromegaly . ^^^ To evaluate the utility of measuring the serum insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) level and the IGF I / IGFBP 3 ratio in the management of acromegalic patients , we comparatively studied the basal concentration of the aforementioned parameters with determination of plasma IGF 1 levels and an index of clinical activity of acromegaly in 16 newly diagnosed acromegalic patients ( aged 34 to 64 years ) before and after hypophysectomy . ^^^ Comparison of individual values between untreated acromegalic patients and control subjects showed that none of the patients had overlapping values for IGF 1 , whereas five of 16 and three of 16 patients had overlapping values for serum IGFBP 3 and IGF I / IGFBP 3 ratio , respectively . ^^^ When we compared the study parameters between the patients who remained with active disease after adenomectomy and the controls , two of 10 had overlapping values for IGF 1 , but six of 10 and five of 10 had overlapping values for serum IGFBP 3 and IGF I / IGFBP 3 ratio , respectively . ^^^ Moreover , comparison of these parameters between cured and active patients after hypophysectomy showed that none had overlapping values for IGF 1 , whereas three of six and one of six had overlapping values for serum IGFBP 3 and IGF I / IGFBP 3 ratio , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) plasma concentrations were also determined in the basal condition before and after NTX treatment . ^^^ NTX treatment reduced fasting insulin levels in patients with EO ( P < . 05 ) and restored a normal GH response to GHRH without affecting IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The confirmed observation in both obese and lean women with and without PCOS of serum IGF 1 and IGFBP 3 levels requires further delineation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and its binding protein IGFBP 3 are sensitive to changes in nutritional status . ^^^ METHODS : Serum concentrations of IGF 1 and IGFBP 3 were measured in 197 subjects ( 108 males , 89 females ; mean age 9 . 69 years , range 0 . 41 17 . 9 years ) and these data were analysed with respect to body mass index , pubertal stage , and clinical status as assessed by Shwachman score and forced expiratory volume in one second ( FEV 1 ) . ^^^ The abnormalities were most obvious during late puberty , when IGF 1 and IGFBP 3 concentrations were significantly reduced compared with those in control subjects matched for pubertal stage . ^^^ CONCLUSIONS : The close relation between declining IGF 1 and IGFBP 3 concentrations and body mass index in patients with cystic fibrosis may simply reflect poor nutritional status and insulin hyposecretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study examined the effects of IGF 1 on serum concentrations of IGF binding protein 3 ( IGFBP 3 ) and GH and assessed how treatment with estradiol and IGF 1 would stimulate adolescent growth in monkeys with normal pituitary function . ^^^ The consequential elevation in serum IGF 1 resulted in a parallel increase in serum IGFBP 3 at 1 and 3 h after treatment with values returning to baseline by 7 h . ^^^ These data confirm that an elevation in IGF 1 increases IGFBP 3 while simultaneously acting in a negative feedback capacity to inhibit GH . ^^^ As found in study 1 , the elevation in serum IGF 1 resulted in a significant increase in serum IGFBP 3 throughout the study in Igf compared with Con females . ^^^ Estradiol administration significantly increased serum IGF 1 and IGFBP 3 levels in both groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , IGFBP 3 ( 200 ng / ml ) inhibited the IGF 1 induced increase in thecal cell numbers by 76 % , and thecal cell progesterone and androstenedione production by 52 % and 89 % , respectively . ^^^ A higher dose of IGF 1 ( i . e . , 100 ng / ml ) overcame the inhibitory effects of IGFBP 3 on IGF 1 induced cell proliferation and on progesterone and androstenedione production by thecal cells . ^^^ As with IGFBP 2 , IGFBP 3 had no effect ( p > 0 . 10 ) on LH induced progesterone or androstenedione production by thecal cells in the absence of IGF 1 . ^^^ Both IGFBP 2 and IGFBP 3 directly inhibited [ 125I ] IGF 1 and 2 binding to thecal cells ; IGFBP 2 was a weaker inhibitor of thecal [ 125I ] IGF 1 and 2 binding than IGFBP 3 . ^^^ These results indicate that IGFBP 3 has a more pronounced inhibitory effect than IGFBP 2 on IGF 1 action in cultured bovine thecal cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Leptin was measured by a newly developed radioimmunoassay and both IGF 1 and IGFBP 3 were measured by commercial radioimmunoassays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effect of 10 min of high intensity cycling exercise on circulating growth hormone ( GH ) , insulin like growth factors 1 and 2 ( IGF 1 and 2 ) , and insulin like growth factor binding protein 3 ( IGF BP 3 ) was studied in nine eumenorrheic women ( age 19 48 yr ) at two different phases of the menstrual cycle . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Exogenous IGFBP 3 either inhibited or slightly stimulated IGF 1 induced cell proliferation when it was coincubated or preincubated with the cells , respectively . ^^^ Preincubation of cells with IGF 1 or long R 3 IGF 1 divergently changed plasminogen activator inhibitor 1 and 2 secretion , but only IGF 1 blocked IGFBP 3 proteolysis . ^^^ IGF 1 , however , had no effect on plasmin induced IGFBP 3 degradation . ^^^ IGF 1 , probably by coupling with IGFBP 3 , can protect it from the action of this protease . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Most of the IGF 1 in plasma circulates in a 150 kDa complex that contains IGF binding protein 3 ( IGFBP 3 ) and an acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Control maternal IGF 1 ( r = 0 . 65 , P < 0 . 0001 ) and IGFBP 3 ( r = 0 . 46 , P = 0 . 001 ) increased with advancing gestational age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To assess the role of insulin like growth factors ( IGFs ) in growth and transformation of normal ( myometrium ) and tumorous smooth muscle cell ( SMC ) tissues , in situ hybridization ( ISH ) analysis for insulin like growth factor 1 and 2 ( IGF 1 and IGF 2 ) mRNAs was combined with detection of IGF peptides , their receptors and IGF binding protein 3 ( IGFBP 3 ) . mRNAs for both IGFs were detected in smooth muscle cells in normal , benign and malignant SMC tissues , together with the IGF peptides , both IGF receptors and IGFBP 3 . ^^^ Low grade leiomyosarcomas contained more IGF 2 mRNAs than myometrium and leiomyoma , fewer type 2 IGF / mannose 6 phosphate receptors and less IGFBP 3 than myometrium and , in addition , fewer IGF 1 mRNAs and type 1 IGF receptors than leiomyoma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After 12 months of study , the 30 children receiving rhGH showed : ( 1 ) greater increase in height ( 9 . 1 + / 2 . 8 vs . 5 . 5 + / 1 . 9 cm , P < 0 . 0001 ) ; ( 2 ) increases in serum levels of IGF 1 , IGF 2 , free IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) ; ( 3 ) a greater decrease in serum IGFBP 1 levels ; and ( 4 ) no significant difference in serum IGFBP 2 levels , when compared to the 14 control patients . ^^^ The change in height SDS after 12 months of rhGH ( +0 . 8 ) in the 30 treated children correlated significantly and positively with serum ALS , IGFBP 3 , total IGF , IGF 1 , IGF 2 and free IGF 1 levels measured during treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Immunological determination of IGFBP 2 , IGFBP 3 , IGF 1 , IGF 2 , interleukin 1 beta ( IL 1 beta ) and tumour necrosis factor alpha ( TNF alpha ) was undertaken in the SF and plasma of 115 patients with rheumatoid arthritis ( RA ; n = 53 ) , osteoarthritis ( OA ; n = 44 ) and other rheumatic disorders . ^^^ Human IGFBP 3 dose dependently inhibited the stimulation of cartilage PG synthesis effected by SF or human IGF 1 . ^^^ The bioactivity of IGF 1 in joints is modulated by IGFBP 3 , which is elevated in RA SF compared to OA SF . ^^^ Elevated IGFBP 3 in RA SF may reduce the availability of IGF 1 to articular chondrocytes , thus interfering with cartilage PG synthesis in RA . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thyroid function tests , IGF 1 and IGFBP 3 were measured on all patients . ^^^ RESULTS : IGF 1 and IGFBP 3 levels were lower in the pituitary patients compared with controls : 104 + / 98 vs 143 + / 37 micrograms . l ( P < 0 . 0001 ) and 2 . 9 + / 0 . 75 vs 3 . 3 + / 0 . 52 mg / l ( P < 0 . 004 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of GH , GHBP , IGF 1 , and IGFBP 3 were determined by RIA . ^^^ In contrast , IGF 1 and IGFBP 3 plasma levels and the IGF 1 to IGFBP 3 ratio increased significantly during 18 months GH therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was a significantly greater increase in IGF 1 and IGFBP 3 in the first month in patients who experienced hGH related adverse events compared with those who did not . ^^^ In the adult onset patients there was an increased risk of adverse events in heavier patients and those who had the greatest increases in IGF 1 and IGFBP 3 at 1 month of therapy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Both IGF 1 and IGFBP 3 reflect spontaneous GH secretion in healthy individuals . ^^^ We have evaluated the clinical usefulness of urinary IGF 1 and IGFBP 3 measurements in the diagnosis of children with disordered growth . ^^^ DESIGN : Serum IGF 1 and IGFBP 3 radioimmunoassays ( RIA ) were developed , and modified for quantitation in urine . ^^^ MEASUREMENTS : IGF 1 and IGFBP 3 in serum and urine were measured by RIA , urinary GH ( uGH ) by immunoradiometric assay ( IRMA ) after dialysis and urinary creatinine by the alkaline picrate method . ^^^ RESULTS : Urine IGF 1 and IGFBP 3 excretion correlated significantly to serum levels of IGF 1 and IGFBP 3 and also to tuGH excretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 is regulated by a number of growth factors / cytokines to which the vascular endothelium is exposed , including IGF 1 stimulation and TGF beta 1 inhibition of IGFBP 3 in cultured endothelial cells . ^^^ These binding elements have also been suggested to be involved in IGF 1 regulation of IGFBP 3 transcription , thus , implying the existence of novel cis acting elements that mediate the IGF 1 stimulation of bovine endothelial cell IGFBP 3 mRNA synthesis ; 3 ) deletion of the GC rich sequence element resulted in a 60 % reduction in basal promoter activity as well as loss of the IGF 1 stimulatory effect ; 4 ) the TGF beta 1 mediated inhibition of IGFBP 3 transcription required sequence element ( s ) beyond 1 . 5 kb of its promoter . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 1 , and IGFBP 3 protein were also associated with the microvascular endothelium , which was accompanied by increased levels of IGFBP 3 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We previously reported that a 16 kDa proteolytic fragment of IGF Binding Protein 3 ( IGFBP 3 ) , which is devoid of affinity for IGFs , inhibits the mitogenic effects of IGF 1 on chick embryo fibroblasts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The response to a GH stimulation test , and levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were also measured . ^^^ The plasma concentrations of IGF 1 and IGFBP 3 were in the lower parts of the normal range . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGF / IGFBP3 molar ratio , and IGF 1 and 2 binding capacities showed no significant change ; however in 5 of 6 patients IGFBP 2 decreased during deflazacort therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concomitant with a decrease in IGFBP 3 and IGFBP 5 release , La3+ caused an increase in [ 125I ] IGF 1 binding to cell associated IGFBPs and type 1 IGF receptors . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Spontaneous GH secretion , IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , their binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) values at the time of clinical diagnosis ( n = 65 ) , after a 25 % decrease in the body mass index ( BMI ) expressed as the SD score ( BMI SD score ; n = 29 ) , and after a diminution of at least 50 % of the initial BMI SD score ( n = 9 ) are reported . ^^^ Serum IGFBP 3 levels correlated positively with IGF 1 , fIGF 1 , and IGF 2 in all groups , but these correlations were weaker in the obese patients at diagnosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report their spontaneous GH secretion and IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , the IGF binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) levels at the time of the clinical diagnosis ( n = 50 ) and after recuperation of between 6 8 % ( n = 42 ) and 10 % or less of the initial weight ( n = 20 ) . ^^^ Independently of GH secretory dynamics , IGF 1 , IGFBP 3 , and GHBP serum levels were all significantly decreased at diagnosis , and only GHBP returned to normal after weight recuperation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was a positive relationship between peak GH levels and serum IGF 1 and IGFBP 3 levels ( r = 0 . 39 ; P = 0 . 009 and r = 0 . 32 ; P = 0 . 03 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , we examined the effects of acute changes in serum insulin on free IGF 1 , total IGF 1 , IGFBP 1 , and IGFBP 3 in 11 healthy subjects . ^^^ IGFBP 3 increased linearly to 20 % above basal by the end of the experiment , and this increase mirrored the decline of free IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in healthy centenarians : relationship with plasma leptin and lipid concentrations , insulin action , and cognitive function . ^^^ In all subjects , fasting plasma IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , leptin , and lipid concentrations were determined ; body composition was assessed by bioimpedance analysis ; and insulin mediated glucose up take was evaluated by euglycemic hyperinsulinemic glucose clamp . ^^^ IGFBP 3 showed a trend similar to IGF 1 , but lower values were present in centenarians than in aged subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting levels of IGF 1 , free IGF 1 , IGF 2 , IGFBP 3 , and insulin increased significantly within 3 months of GH treatment , whereas IGFBP 1 , IGFBP 2 , and IGFBP 6 showed no change . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Immunostaining of sections of normal and cancer tissue was negative for IGF 1 and IGF 2 ; IGFBP 2 was positive in 2 out of 10 cancer tissues and 7 out of 10 normal tissues ; IGFBP 3 was positive in 7 out of 10 cancer tissues and 7 out of 10 normal tissues ; and IGFBP 4 was positive in 5 out of 10 cancer tissues and 6 out of 10 normal tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGFBP 2 , but not those of IGF 1 or IGFBP 3 , were negatively correlated with body mass index and body weight . ^^^ Finally , serum levels of IGFBP 3 , but not those of IGF 1 , were positively correlated with serum levels of total cholesterol and triglyceride . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN , PATIENTS AND MEASUREMENTS : IGF 1 , free IGF 1 , 24 hour urinary GH ( uGH ) , serum IGFBP 3 and 24 hour urinary IGFBP 3 ( uIGFBP 3 ) were measured in 12 patients with untreated active acromegaly , in 29 patients who had been treated but were not cured , in 13 patients with cured acromegaly and in 14 healthy control subjects , and compared with the results of the OGTT . ^^^ In patients with untreated active acromegaly IGF 1 , free IGF 1 , uGH and IGFBP 3 , but not uIGFBP 3 , were significantly higher than in healthy individuals ( P < 0 . 0001 ) . ^^^ In patients with acromegaly there was a significant correlation between nadir GH levels in OGTT and IGF 1 ( r = 0 . 71 ) , free IGF 1 ( r = 0 . 76 ) , IGFBP 3 ( t = 0 . 73 ) and uGH ( r = 0 . 81 ) ( P < 0 . 0001 ) , but no correlation with uIGFBP 3 . ^^^ IGF 1 , free IGF 1 , IGFBP 3 and uGH were useful in the diagnosis of acromegaly , but of limited value in the follow up of acromegalic patients after treatment . ^^^ The determination of free IGF 1 , which has yet not been investigated in acromegaly , offered no advantage over that of total IGF 1 and IGFBP 3 ; uIGFBP 3 was not useful in the diagnosis of acromegaly . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating concentrations of fetal , but not maternal , IGF 1 increased with treatment ( approximately 80 to approximately 1015 ng / ml ) , and there was no evidence of a change in serum IGF 2 or an increase in IGF binding protein 3 compared with historical control values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Prognostic value of insulin like growth factor 1 IGF 1 and its binding protein IGFBP 3 in alcoholic liver disease ] . ^^^ The liver is the major source of IGF 1 and its binding protein , IGFBP 3 . ^^^ Circulating concentrations of IGF 1 and IGFBP 3 are decreased in patients with chronic liver disease and correlate with the severity . ^^^ The aim of this study was to assess the additional prognostic value of IGF 1 and IGFBP 3 in patients entered in a large multicentre study ( EMALD ) . ^^^ When IGF 1 or IGFBP 3 were added into this model , a Cox regression analysis showed that either had a significant independent prognostic value . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 did not modulate the activity of the IGFBP 4 protease in solution but was able to prevent the inhibition seen with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of total insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) reflect endogenous GH secretion in healthy children , which makes them good diagnostic markers for screening of GH deficiency ( GHD ) in short children , although some controversy still exists . ^^^ In adults , the diagnostic value of total IGF 1 and IGFBP 3 determinations in patients suspected of GHD has only been reported in a few studies , whereas no previous reports on the diagnostic value of free IGF 1 levels in adults suspected of GHD exist . ^^^ We studied the diagnostic value of free IGF 1 in relation to total IGF 1 and IGFBP 3 determinations in adults who were suspected of GHD . ^^^ We found that determination of the free IGF 1 serum concentration may predict the outcome of a GH provocative test in adults suspected of GHD , but that a single determination of free IGF 1 offered no significant advantage compared to determination of total IGF 1 or IGFBP 3 serum levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Children with CRF who are treated with GH show catch up growth that correlates positively with the increase in each component of the 150 kd serum ternary complex ( acid labile subunit , IGFBP 3 , IGF 1 , and IGF 2 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess the clinical utility of growth hormone binding protein ( GHBP ) , along with growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) , levels in the evaluation of short stature . ^^^ IGF 1 standard deviation scores ( SDS ) and IGFBP 3 SDS were positively correlated ; both increased during GH therapy . ^^^ There was a weak positive correlation between log peak GH levels and both IGF 1 SDS and IGFBP 3 SDS and a weak negative correlation between log peak GH levels and GHBP SDS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of type 1 procollagen C terminal propeptide , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 in obese children and adolescents : relationship to gender , pubertal development , growth , insulin , and nutritional status . ^^^ We measured fasting serum levels of type 1 procollagen C terminal propeptide ( PICP ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) in obese children and adolescents ( obese subjects [ OS ] ) to evaluate their relationship to growth , gender , pubertal stage , and weight excess ( WE ) . ^^^ Using stepwise analysis , a positive correlation between IGF 1 and IGFBP 3 was observed in prepubertal but not in pubertal NWS . ^^^ In prepubertal NWS , PICP and SDS of body mass index ( BMI ) correlated with IGF 1 , accounting for 12 . 9 % of the variation , and SDS of BMI correlated with IGFBP 3 , explaining 27 . 8 % of the variation . ^^^ In conclusion , we demonstrated that IGF 1 and IGFBP 3 are influenced by age , gender , sexual development , and nutritional status . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study was performed to investigate the role of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 in age dependent bone loss in postmenopausal Japanese women . ^^^ Serum levels of IGF 1 , IGFBP 2 , and IGFBP 3 were measured by radioimmunoassay . ^^^ BMD at all sites as well as serum levels of IGF 1 and IGFBP 3 declined with age , while the serum IGFBP 2 level increased with age . ^^^ Serum IGFBP 3 and 2 levels were positively and negatively correlated with the serum IGF 1 level , respectively . ^^^ Serum IGF 1 and IGFBP 3 levels showed positive correlationship with BMD at any site , particularly at the midradius , while the serum IGFBP 2 level showed negative correlation with BMD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To evaluate the effects of exogenous recombinant human IGF 1 on circulating concentrations of IGF 1 binding protein 1 ( IGFBP 1 ) , IGF 1 binding protein 3 ( IGFBP 3 ) , and growth hormone in the critically ill patient ; to assess the safety of the subcutaneous administration of 40 microg / kg of recombinant human IGF 1 in these patients ; and to investigate any effect this dose might have on nitrogen balance , creatinine clearance , and serum electrolyte and lipid concentrations . ^^^ Serum concentrations of IGF 1 , IGFBP 1 , IGFBP 3 , growth hormone , and insulin were measured by radioimmunoassay . ^^^ IGFBP 3 concentrations also increased after recombinant human IGF 1 injection , and at 3 to 4 hrs were significantly elevated , from 30 mins ( p = . 04 ) to 8 hrs ( p = . 04 ) . ^^^ There was marked between patient variability in changes in circulating IGF 1 , and IGFBP 1 , and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In both GHD and controls , free IGF 1 correlated significantly ( P < 0 . 05 ) with total IGF 1 ( GHD r = 0 . 78 ; controls r = 0 . 42 ) , IGFBP 1 ( GHD r = 0 . 67 ; controls r = 0 . 46 ) and the molar ratio of total IGF 1 over IGFBP 3 ( GHD r = 0 . 58 ; controls r = 0 . 62 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in children with precocious puberty treated with gonadotropin releasing hormone analog without or in combination with cyproterone acetate . ^^^ In order to assess the behavior of growth hormone , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in girls with central precocious puberty treated with gonadotropin releasing hormone ( GnRH ) analog therapy , we studied 14 girls with this condition , the patients were subdivided into two groups , according to the therapy followed . ^^^ During the two years of gonadal suppression , Group A patients showed a significant decrease in IGF 1 and IGFBP 3 levels , while in Group B there was no significant change in IGF 1 ; moreover , in Group B , IGFBP 3 levels increased significantly compared to baseline values during the first year of treatment ( 4532 . 3 + / 167 micrograms / l vs . 5410 . 3 + / 169 micrograms / l ; p < 0 . 05 ) and decreased significantly at the end of the second year of treatment ( 3816 . 1 + / 189 micrograms / l vs . 5410 . 3 + / 169 micrograms / l ; p < 0 . 01 ) . ^^^ Our study shows that the two different treatments of precocious puberty ( with and without cyproterone acetate ) have different effects on IGF 1 and IGFBP 3 , and suggests that these growth factors are under different metabolic regulation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The samples were assayed for IGF 1 , IGF 2 , IGF binding protein 3 ( IGF BP 3 ) , GH and estradiol . ^^^ We can conclude that ( 1 ) there is a negative correlation between GH and IGF 1 plasma levels in patients undergoing controlled ovarian hyperstimulation ( COH ) IVF , when levels of estradiol and GH are elevated ; ( 2 ) plasma levels of IGF 1 under ovarian hyperstimulation are probably regulated by a multifactorial system ; and ( 3 ) no correlation was found between the plasma levels of IGF 1 and those of IGF 2 and IGF BP 3 in all patient groups . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the 39 blood samples IGF 1 , IGFBP 3 , TSH , T 4 and T 3 were measured . ^^^ Higher GH peaks ( NS ) , IGF 1 ( P , 0 . 01 ) and IGFBP 3 ( P < 0 . 01 ) levels were found in women with regular menstrual cycles , compared to women using sex steroid substitution and amenorrhoeic women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , the data point to IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 as putative mediators of relaxin induced uterine growth in the pig . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The simulation shows that higher concentrations of IGFBP 3 would increase the estimate of MCR for free IGF 1 by reducing free IGF 1 concentration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We aimed to study the possible roles of growth hormone , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) in this growth pattern . ^^^ Serum growth hormone and plasma IGF 1 and IGFBP 3 concentrations were measured . ^^^ The prepubertal children with hydrocephalus had lower IGF 1 ( p = 0 . 002 ) and IGFBP 3 concentrations ( p < 0 . 001 ) than the controls , and the pubertal children had four times lower basal growth hormone concentrations ( p < 0 . 001 ) . ^^^ The prepubertal patients on antiepileptic treatment , carbamazepine in most cases ( 73 % ) , had higher IGF 1 ( p = 0 . 01 ) and IGFBP 3 concentrations ( p = 0 . 03 ) than those who had never been treated with antiepileptic drugs , but still lower IGFBP 3 levels than the controls ( p = 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These results suggest that increased protease activity in maternal blood may be involved in the fetal growth indirectly by reducing the binding activity of IGFBP 3 to IGF 1 , and that protease activity in maternal blood may be derived from decidua that is regulated by placental hormones . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We assessed circulating levels of IGF 1 and IGFBP 1 and IGFBP 3 in 23 patients with endometrial cancer , 11 patients with uterine cervical cancer and 27 healthy control women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with native IGFBP 3 , pure IGFBP 3 ( 29 ) has a 4 10 fold lower affinity for IGF 2 and a 200 fold lower affinity for IGF 1 . ^^^ Consistent with the binding data , IGFBP 3 ( 29 ) inhibited IGF 2 stimulated thymidine incorporation in chondrosarcoma cells , but was a less potent inhibitor than native IGFBP 3 ; also , native IGFBP 3 clearly inhibited IGF 1 stimulated thymidine incorporation in chondrosarcoma cells and potentiated IGF 1 stimulated aminoisobutyric acid uptake in bovine fibroblasts , but higher concentrations of IGFBP 3 ( 29 ) had no effect on these IGF 1 actions . ^^^ Thus , the 29 kD IGFBP 3 form that accumulates in CRF serum and extravascular spaces is an IGFBP 3 fragment that may modulate IGF 2 , but not IGF 1 , effects on target tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , men with idiopathic osteoporosis were compared with healthy men , with respect to bone histomorphometry and to serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 and IGFBP 3 , and 24 h urinary excretion of GH . ^^^ In the patients , serum concentrations of IGFBP 3 were reduced , compared with controls , with a 46 % lower mean value ; whereas levels of IGF 1 , IGF 2 , IGFBP 2 , and GH were similar in the two groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Free and total insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 and their relationships to the presence of diabetic retinopathy and glomerular hyperfiltration in insulin dependent diabetes mellitus . ^^^ Serum free and total IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 levels were measured in 56 insulin treated IDDM patients and 52 healthy sex and age matched controls . ^^^ Fasting free IGF 1 , total IGF 1 , and IGFBP 3 levels were significantly lower in IDDM patients than in age and sex matched healthy controls ( free IGF 1 , P < 0 . 005 ; total IGF 1 , P < 0 . 001 ; IGFBP 3 , P = 0 . 001 ) , whereas IGFBP 1 levels were higher ( P < 0 . 001 ) . ^^^ In IDDM subjects , decreases in free IGF 1 , total IGF 1 , and IGFBP 3 levels with age were observed ( free IGF 1 , r = 0 . 27 and P = 0 . 05 ; total IGF 1 , r = 0 . 52 and P < 0 . 001 ; IGFBP 3 , r = 0 . 37 and P = 0 . 005 ) . ^^^ Total IGF 1 and IGFBP 3 levels were positively related to GFR ( total IGF 1 , r = 0 . 35 and P < 0 . 05 ; IGFBP 3 , r = 0 . 28 and P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth response to growth hormone ( GH ) treatment relates to serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in short children with various GH secretion capacities . ^^^ The purpose of the study was to evaluate the relationship between the 1 yr ( n = 193 ) and 2 yr ( n = 128 ) growth response and the individual serum concentrations of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) before and during GH treatment . ^^^ Their serum levels of IGF 1 and IGFBP 3 were low before treatment compared to those in an age related reference group of prepubertal children and increased significantly from the start to 1 month of GH treatment . ^^^ In univariate analyses the highest correlation coefficients to the 2 yr growth response were found to be vs . the following variables from the start of treatment : IGF 1 SD score ( r = 0 . 49 ) , log maximum GH concentration ( log GHmax ) during the arginine insulin tolerance test ( r = 0 . 47 ) , difference between the height SD score of the individual child and the midparental height SD score ( diffSD score ; r = 0 . 45 ) , IGFBP 3 SD score ( r = 0 . 39 ) , age ( r = 0 . 30 ) , short term change in IGFBP 3 SD score ( r = 0 . 37 ) , and IGF 1 SD score ( r = 0 . 34 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We analyzed body composition by bioelectrical impedance and used standard radionuclide dilution methods to measure RCM and plasma volume ( PV ) along with measuring full blood count , ferritin , vitamin B 12 , red cell folate , IGF 1 , IGF binding protein 3 , and erythropoietin in 13 adult patients with GHD as part of a 3 month , double blind , placebo controlled trial of GH ( 0 . 036 U / kg . day ) . ^^^ Serum IGF 1 and IGF binding protein 3 concentrations increased by 3 . 0 + / 0 . 43 ( P < 0 . 001 ) and 1 . 3 + / 0 . 15 ( P < 0 . 001 ) SD , respectively , but the erythropoietin concentration was unchanged after GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 were elevated 3 to 13 fold in nondiabetic retinal ischemia and 1 . 5 to 3 fold in PDR , indicating that the changes were not restricted to diabetes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aims of this investigation were ( 1 ) to examine IGF binding protein 3 ( IGFBP 3 ) mRNA levels in candidate tissues which might be important sources for blood IGFBP 3 ( liver and skin ) and in a target tissue for IGF 1 action ( skeletal muscle ) , and ( 2 ) to examine the effects of a single dose ( 500 micrograms ) of GH or IGF 1 on IGFBP 3 message levels in these tissues since temporal responses ( 4 , 8 and 24 h after the single subcutaneous dose of peptide to GH deficient dwarf rats ) would indicate which peptide is the primary modulator of IGFBP 3 synthesis . ^^^ Circulating IGF 1 and IGFBP 3 concentrations were significantly increased ( P < 0 . 05 ) by IGF 1 and GH . ^^^ GH treatment increased liver IGFBP 3 mRNA levels by 4 h ( P < 0 . 001 over the 24 h ) whereas IGF 1 had no effect . ^^^ Similarly , GH , but not IGF 1 , increased muscle IGFBP 3 mRNA levels ( P < 0 . 001 for the 24 h study period ) . ^^^ However , both IGF 1 and GH induced increases in skin IGFBP 3 mRNA abundance throughout the 24 h period ( P < 0 . 001 and P < 0 . 01 respectively ) and skin IGFBP 3 message abundance was greater that in the liver . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Western ligand blotting showed that IGFBP 3 accounted for approximately 50 60 % of the plasma IGF 1 binding capacity in sheep respectively both before and during GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Long R 3 IGF 1 , an IGF 1 analogue with greatly reduced affinity for IGFBPs yet similar affinity for IGF 1 receptors , was a more potent inhibitor of IGFBP 3 induced and ICI induced apoptosis than IGF 1 . ^^^ These results suggest that IGFBP 3 enhances apoptosis by reducing bioavailability of ligands for the IGF 1 receptor and suggest that modulation of IGFBP 3 expression by ICI contributes to apoptosis induced by this compound . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Serum and urinary concentrations of GH were only raised on the days of administration whereas , following cessation of somatropin , the increases in the serum concentrations of IGF 1 and IGFBP 3 were sustained for at least 1 day ( 30 h ) . ^^^ The concentration ratios of IGFBP 3 to IGFBP 2 and IGF 1 to IGFBP 2 increased markedly with administration and both ratios were still significantly augmented compared with basal values 30 h after the last administration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , the expression of mRNAs coding for IGF 1 and the binding proteins IGFBP 2 and IGFBP 3 is augmented in response to unilateral ischemia in animal models . ^^^ METHODS : Plasma IGF 1 and IGFBP 3 were measured sequentially in 20 patients with acute ischemic stroke ( within 24 hours and 3 , 5 , and 10 days thereafter ) . ^^^ RESULTS : Plasma IGF 1 and IGFBP 3 plasma concentrations after acute cerebral ischemia were strikingly lower than those in control subjects and healthy individuals reported in the literature . ^^^ Plasma IGF 1 levels in patients with large infarcts were significantly statistically lower than those in control subjects ( P < . 05 ) , and plasma IGFBP 3 levels were significantly lower than those in control subjects on days 5 and 10 . ^^^ CONCLUSIONS : IGF 1 and IGFBP 3 plasma levels are decreased in patients after cerebral ischemia . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 levels correlated significantly with IGFBP 3 levels ( r = 0 . 71 ) , birthweight ( r = 0 . 48 ) and birth length ( r = 0 . 37 ) . ^^^ According to multiple regression analysis free IGF 1 levels were only dependent upon total IGF 1 , IGFBP 2 and IGFBP 1 , whereas IGFBP 3 levels did not contribute to the variance of free IGF 1 concentrations in venous cord blood . ^^^ When IGF 1 and IGFBP 3 levels were analysed with respect to gestational age a biphasic pattern with maxima at 270 d was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have established the age and sex matched reference values of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) in Japanese . ^^^ Both IGF 1 and IGFBP 3 had the similar diagnostic values for GH deficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum calcium , phosphorus , 1 , 25 ( OH ) 2D , immunoreactive intact parathyroid hormone ( PTH ) , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , tubular reabsorption of phosphate ( TMP / GFR ) were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OP 1 alone or together with IGF 1 increased the steady state IGFBP 3 mRNA level and reduced the steady state mRNA levels of IGFBP 4 , 5 , and 6 . ^^^ IGF 1 alone did not change the steady state mRNA levels of IGFBP 3 , 4 , and 6 , but elevated that of IGFBP 5 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant correlations were seen with spontaneous GH secretion or measurements of IGF 1 , IGF binding protein 3 , insulin and leptin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MATERIALS AND METHODS : ELISAs were used to determine levels of epidermal growth factor ( EGF ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and heparin binding epidermal growth factor like growth factor ( HB EGF ) in urine specimens from women with IC , asymptomatic women without bladder disease , and women with bacterial cystitis . ^^^ In contrast , urine EGF , IGF 1 , and IGFBP 3 levels were all significantly elevated in IC patients compared to asymptomatic controls . ^^^ Further , the amounts of urine EGF and IGF 1 were also elevated in IC patients as compared to patients with bacterial cystitis , and urine IGFBP 3 levels were significantly elevated when expressed per milligram of urine creatinine . ^^^ CONCLUSIONS : These findings indicate that complex changes in the levels of urine epithelial cell growth factors ( EGF , IGF 1 , and HB EGF ) and a growth factor binding protein ( IGFBP 3 ) are associated with IC . ^^^ While EGF , IGF 1 , and IGFBP 3 levels are either the same or increased in the urine of IC patients as compared to patients with bacterial cystitis or asymptomatic controls , HB EGF levels are significantly decreased in the urine of IC patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGFBP 3 , for instance , are important indicators of states of altered growth hormone secretion and action , as well as being important during treatment with growth hormone or IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similarly , recombinant IGFBP 3 , the major circulating IGFBP that in kidney is produced close to the proximal tubules , also inhibited IGF 1 stimulated DNA synthesis in cultured rabbit proximal tubular cells and in cultured opossum kidney ( OK ) cells . ^^^ IGFBP 3 also inhibited basal DNA synthesis in OK cells in the absence of added IGF 1 , suggesting that this IGFBP may have an IGF 1 independent action . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of GH , IGF 1 , IGFBP 3 , and insulin were measured by radioimmunoassay and 24 hour urinary nitrogen by the Kjeldahl technique . ^^^ RESULTS : IGF 1 levels increased only in the GH treated group ( mean , 69 . 2 + / SE 7 . 0 to 170 . 6 + / 48 . 8 ng / mL ; P < 0 . 05 ) together with IGFBP 3 ( 1 . 65 + / 0 . 3 to 2 . 94 + / 0 . 6 mg / L ; P < 0 . 005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both human and rat mesangial cells grown on BSA AGE showed increased IGF 1 and total and bioactive TGF beta medium levels and enhanced IGF 1 , IGF 2 , and TGF beta 1 gene expression , compared with cells grown on BSA , whereas total IGFBP and IGFBP 3 medium content , IGF receptor density and affinity , and IGF 1 receptor transcripts were unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Human trabecular osteoblasts produce mainly IGF 2 , IGFBP 3 and minute quantities of IGF 1 in culture . ^^^ No differences in the basal or GH stimulated production of IGF 1 , IGF 2 or IGFBP 3 were found between osteoporotic patients and age matched normals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of extracorporeal ( urinary plus peritoneal ) losses of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) on their respective serum levels were studied in 10 adult patients ( aged 42 74 years ) with end stage renal failure and residual renal function of 0 4 . 5 mL / min . ^^^ Morning serum , 24 hour urine , and 8 hour overnight peritoneal concentrations of IGF 1 and IGFBP 3 were measured by radioimmuno ( Incstar ) and immunoradiometric ( Active ) assays . ^^^ Extracorporeal losses of IGF 1 accounted for about 4 % of the daily production rate of this polypeptide , and the peritoneal and urinary concentrations of IGFBP 3 did not exceed 4 % and 14 % , respectively , of their serum levels . ^^^ Serum concentrations of IGF 1 ( 227 . 7 + / 64 . 2 micrograms / L ) and IGFBP 3 ( 5 . 3 + / 2 . 4 mg / L ) were not significantly correlated with extracorporeal , peritoneal , or urinary losses of these proteins or with residual renal function . ^^^ We suggest that extracorporeal losses of IGF 1 and IGFBP 3 in adult patients on CAPD do not influence their serum levels and that IGF 1 may therefore be used as a marker of malnutrition . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone secretion and circulating insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 concentrations in children with sickle cell disease . ^^^ We undertook this study to test the hypothesis that this impaired growth is associated with abnormalities of the growth hormone ( GH ) / insulin like growth factor 1 ( IGF 1 ) / IGF binding protein 3 ( IGFBP 3 ) axis in 21 children with SCD and that SCD is associated with GH resistance . ^^^ Nine of 21 children with SCD had a defective GH response to both clonidine and glucagon provocation ( peak < 10 micrograms / L ) ; these children differed from the 12 others in having slower linear growth velocity ( GV and GVSDS ) , lower circulating concentrations of IGF 1 and IGFBP 3 , and either partial or complete empty sellae in computed tomographic scans of the hypothalamic pituitary area . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Age related differences in serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in congenital hypothyroidism . ^^^ We investigated age related differences in serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels in 43 patients with primary congenital hypothyroidism . ^^^ Patients diagnosed in the first month of life did not display a significant difference in serum IGF 1 and IGFBP 3 levels compared to age matched controls ( p > 0 . 05 ) . ^^^ However , in groups 2 to 5 , IGF 1 and IGFBP 3 levels were significantly lower than in controls ( p < 0 . 05 ) . ^^^ Thyroid hormone replacement therapy increased serum IGF 1 and IGFBP 3 levels significantly in 26 hypothyroid children ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) , and tests of GH stimulation and spontaneous secretion , led to the following subgroups : severe isolated GHD ( SIGHD ) ( GH < 7 ng / ml ) ( n = 21 ) ; partial , isolated GHD ( GH 7 10 ng / ml ) ( n = 22 ) ; multiple pituitary hormone deficiency ( MPHD ) ( n = 13 ) ; neurosecretory dysfunction ( n = 10 ) ; non classifiable diagnosis ( NC ) ( n = 13 ) ; idiopathic short stature ( n = 9 ) ; and intra uterine growth retardation ( n = 3 ) . ^^^ PHT SDS correlates with IGF 1 SDS ( r = 0 . 48 , P < 0 . 0001 ) , IGFBP 3 SDS ( r = 0 . 46 , P < 0 . 0001 ) and the highest peaks in tests of GH stimulation and GH spontaneous secretion ( r = 0 . 36 , P < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After 3 12 months of therapy there was a marked growth spurt , higher nocturnal GH levels and an increase in both IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
C peptide , IGF 1 , IGFBP 3 plasma levels and the IGF I / IGFBP 3 molar ratio were also determined . ^^^ MEASUREMENT : GH was determined by the IRMA method ; insulin , C peptide , IGF 1 and IGFBP 3 were assayed by the RIA method . ^^^ For molar comparison between IGF 1 and IGFBP 3 we have considered 30 . 5 kDa the molar weight of IGFBP 3 . ^^^ No modifications were found in IGF 1 , IGFBP 3 and IGF I / IGFBP 3 molar ratio . ^^^ Moreover , NTX affected neither the insulin response to OGTT or IGF 1 , IGFBP 3 and IGF I / IGFBP 3 molar ratio in a group of six lean controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIMS : To evaluate the developmental pattern of fetal growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , GH binding protein ( GHBP ) and IGF binding protein 3 ( IGF 3 ) ; to determine the implications for fetal growth . ^^^ METHODS : Serum GH , IGF 1 , GHBP and IGFBP 3 were measured in 53 fetuses , 41 aged 20 26 weeks ( group A ) and 12 aged 31 38 weeks ( group B ) . ^^^ GH , IGF 1 and IGFBP 3 were determined by radioimmunoassay . ^^^ Gestational age correlated positively with IGF 1 concentrations ( P < 0 . 0001 ) and IGFBP 3 ( P < 0 . 0001 ) and negatively with GH ( P < 0 . 0001 ) . ^^^ Multiple regression analysis showed a negative correlation between GH : IGF 1 ratio and fetal growth indices CONCLUSIONS : The simultaneous evaluation of fetal GH , IGF 1 , IGFBP 3 and GHBP suggests that the GH IGF 1 axis might already be functional in utero . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and its major binding protein , IGFBP 3 , both rose during pubertal development ; however , a simultaneous rise in the IGF 1 : IGFBP 3 molar ratio suggests other factors may enhance the bioactivity of IGF 1 during puberty . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGFBP 3 were measured before and up to 48 h after human recombinant growth hormone injection . ^^^ IGF 1 levels at 24 h and beyond correlated significantly with the nutritional status , the Child Pugh score , and the basal levels of GH binding protein and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When endogenous secreted IGFBPs were removed , cell lines were consistently more sensitive to IGF 1 than des ( 1 , 3 ) IGF 1 suggesting that IGFBP 3 associated with the cell layer enhances responses to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factors ( IGF 1 and IGF 2 ) and their binding protein ( IGFBP 3 ) are not elevated in pancreatic cancer . ^^^ CONCLUSION : Serum levels of IGF 1 , IGF 2 , and IGFBP 3 are not elevated in pancreatic cancer and do not appear to have a significant role in glucose homeostasis in this group of patients . ^^^ METHODS : Serum levels of IGF 1 , IGF 2 , and IGFBP 3 were measured by RIA in 20 patients with pancreatic cancer and 20 age matched healthy control subjects and correlated with serum glucose , C peptide , and glucose tolerance . ^^^ RESULTS : No significant difference was observed in serum levels of IGF 1 ( 13 vs 17 nmol / L , respectively ) , IGF 2 ( 0 . 67 vs 0 . 91 U / mL ) , or IGFBP 3 ( 2 . 3 vs 2 . 3 mg / L ) between the two groups of patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The proband is a 3 . 5 year old Druse girl with severe short stature ( height SDS 5 . 1 ) , high GH ( 250 micrograms / l ) , low IGF 1 ( 2 . 7 nmol / l ) and IGFBP 3 ( 410 micrograms / l ) , both unresponsive to exogenous GH . ^^^ Serum IGF 1 and IGFBP 3 levels were normal in all the subjects , with the exception of an aunt ( IGF 1 3 . 9 nmol / l ) and the proband ' s sister ( IGFBP 3 460 micrograms / l ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated serum GH , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) levels in 42 obese and 40 non obese healthy children aged 6 14 years . ^^^ Serum IGF 1 levels were studied by immunoradiometric assay ( IRMA ) and IGFBP 3 levels by radioimmunoassay ( RIA ) . ^^^ IGF 1 levels were significantly higher in the obese group ( p < 0 . 05 ) , but IGFBP 3 levels were not different from the control group . ^^^ IGF 1 and IGFBP 3 levels were significantly higher in the obese pubertal children than in the obese prepubertal ones ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In competitive binding experiments , IGFBP 3 was as potent as unlabelled IGF 1 in displacing its truncated analogue , 125I des ( 1 3 ) IGF 1 , which has weak affinity for IGFBPs , from its binding to the cell surface . ^^^ At 10 nM , IGFBP 1 was ineffective where IGFBP 3 provoked 90 % displacement of 125I des ( 1 3 ) IGF 1 , but it was equally potent in displacing 125I IGF 1 . ^^^ At the same concentration , binding of 125I des ( 1 3 ) IGF 1 to free IGFBP 3 in the supernatant was only 2 % . ^^^ After pre incubation of the cells with 125I des ( 1 3 ) IGF 1 , low concentrations of IGFBP 3 were as potent as IGF 1 in dissociating IGFR 1 bound ligand . ^^^ After pre incubation of cells with IGFBP 3 , washing and then incubation with 125I des ( 1 3 ) IGF 1 , inhibition by low IGFBP 3 concentrations was suppressed , but some degree of inhibition by high concentrations persisted . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Acromegaly : the significance of serum total and free IGF 1 and IGF binding protein 3 in diagnosis . ^^^ We have studied the physiological and clinical relevance of measurements of serum total and free IGF 1 and IGF binding protein 3 ( IGFBP 3 ) in 57 previously untreated patients with active acromegaly ( 32 males , 25 females ; mean age 47 years ) as compared with sex and age matched normal healthy controls . ^^^ Serum total and free IGF 1 , but not IGFBP 3 , are suitable biochemical parameters for screening for acromegaly . ^^^ In acromegalics , the mean 24 h serum GH , total IGF 1 and IGFBP 3 levels tend to decrease with age . ^^^ However , in our series of patients , mean 24 h serum GH levels , IGFBP 3 , total and free IGF 1 do not correlate with disease activity in acromegaly . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone stimulates IGF 1 secretion and that of its major binding protein IGFBP 3 . ^^^ There is overlap in the values of IGF 1 and IGFBP 3 between normal subjects and patients on treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before weaning , plasma IGF 1 concentrations were higher in rams than ewes , and plasma concentrations of IGF 2 and IGF binding protein 3 ( IGFBP 3 ) also tended to be higher in rams than in ewes . ^^^ Plasma concentrations of IGF 1 and IGFBP 3 did not differ between rams and ewes at 100 days of age , but plasma IGF 2 was higher in rams than in ewes at this time . ^^^ Since circulating concentrations of GH were higher in rams than in ewes at 100 days of age , this implies that the restricted feed intake blocked the IGF 1 and IGFBP 3 responses to GH . ^^^ We conclude that sex differences in circulating IGF 1 and IGFBP 3 concentrations in the growing lamb alter with age , and are not present when nutrition is restricted . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Height velocity , serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and GH binding protein ( GHBP ) concentrations , and GH responses to GHRP 2 by i . v . bolus and intranasal spray were examined during treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Physical growth and the serum growth factors , insulin growth factor 1 ( IGF 1 ) and its binding protein ( IGFBP 3 ) were measured weekly during dexamethasone treatment and for 3 weeks after stopping therapy in 10 ventilated babies [ median ( range ) birth weight 860 g ( 640 1210 ) ; median ( range ) gestational age 26 weeks ( 24 29 ) ] with bronchopulmonary dysplasia ( BPD ) . ^^^ Mean serum IGF 1 ( 1 . 57 + / 0 . 13 versus 3 . 56 + / 0 . 41 nmol / L ) and IGFBP 3 ( 0 . 94 + / 0 . 03 versus 1 . 12 + / 0 . 05 mg / L ) levels also increased off treatment . ^^^ Protein intake ( g / kg / day ) was significantly correlated ( P < 0 . 01 ) with weight gain ( r = 0 . 28 ) , changes ( TBL ) ( r = 0 . 32 ) , serum IGF 1 levels ( r = 0 . 60 ) , and IGFBP 3 levels ( r = 0 . 37 ) . ^^^ Poor growth during steroid treatment is associated with lower IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum ALS fractionated by gel filtration high performance liquid chromatography eluted in a single peak at approximately 150 kDa with IGF 1 and IGFBP 3 , but appeared at about 400 500 kDa after sample acidification and fractionation under acidic condition . ^^^ ALS in adult sera more tightly correlated with IGFBP 3 than IGF 1 or IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine whether peptides of the insulin like growth factor ( IGF ) system might be useful indicators of nutritional adequacy in premature infants , we studied 50 premature ( 25 34 weeks gestation ) infants prospectively to define the relationship between nutrient intake and serum concentrations of IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 . ^^^ Nutrient intake was quantified from daily logs ; weight was determined daily , and measurements of IGF 1 , IGFBP 2 , and IGFBP 3 in serum were made twice weekly . ^^^ Unlike IGF 1 , variation in the amount of protein supplied did not change IGFBP 3 . ^^^ In contrast to IGF 1 and IGFBP 3 , IGFBP 2 declined as the length of gestation increased ( 56 . 12 + / 16 . 92 ng / mL . week ; P = 0 . 001 ) and with each additional day of life ( 7 . 57 + / 2 . 44 ng / mL . day ; P = 0 . 003 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Endocrine evaluation of the growth hormone ( GH ) IGF 1 axis revealed elevated GH secretion , undetectable serum IGF 1 and normal serum IGF binding protein 3 , acid labile subunit , and GH binding activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 were also reduced in short children born SGA , compared with the reference groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , plasma levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 revealed a normal developmental increase and were not significantly altered by GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Follicle stimulating hormone , luteinizing hormone ( LH ) , oestradiol , progesterone , IGF 1 , IGFBP 3 , sex hormone binding globulin , dihydroepiandrosterone sulphate and GH were determined in all samples , whereas insulin and IGFBP 1 were determined in fasted samples only . ^^^ Therefore , the menstrual cycle does not need to be considered when evaluating IGF 1 or IGFBP 3 serum values in women suspected to have GH deficiency . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In dogs , this results in high plasma concentrations of GH and a concomitant increase in plasma IGF 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , the addition of CF CM to PTC increased cell associated IGFBP 3 on PTC surfaces , without changes in IGF 1 receptor numbers or affinity and without changes in PTC mRNA for IGFBP 3 . ^^^ Des ( 1 3 ) IGF 1 , an analog that binds to the IGF 1 receptor but not to IGFBPs , provided a less potent stimulus for PTC growth compared with IGF 1 , indicating that cell associated IGFBP 3 facilitates the action of IGF 1 on PTC . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The bioavailability of IGF 1 to the tissues is modulated by IGF binding proteins ( IGFBPs ) , and higher circulating levels of IGF 1 and lower levels of IGFBP 3 have been reported in breast cancer patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 , IGFBP 1 , IGFBP 3 , and insulin were measured by specific immunoassays . ^^^ RESULTS : Compared with the controls , patients with PCOS had significantly higher insulin levels , similar IGF 1 and IGFBP 3 levels , and significantly lower IGFBP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 induced IGFBP 3 and IGFBP 5 proteins in a time dependent manner without an increase in the corresponding mRNAs . ^^^ Recombinant human IGFBP 3 ( rhIGFBP 3 ) was then tested for its effect on IGF 1 induced mitogenesis in HSCs . rhIGFBP 3 inhibited IGF 1 stimulated DNA synthesis in a dose dependent manner , with a peak effect observed at 25 nM IGFBP 3 . ^^^ IGF 1 and TGF beta differentially regulate IGFBP 3 , IGFBP 4 , and IGFBP 5 expression , which , in turn , may modulate the in vitro and in vivo action of IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In both children and adults , the biochemical indices utilized to predict and / or monitor response to GH therapy have included : ( 1 ) nonspecific indices : glucose , insulin , urea , protein synthesis , lipid metabolism , and lipoproteins ; ( 2 ) more specific indices of the GH IGF axis : GH binding protein , IGF 1 , IGFBP 3 , and acid labile subunit ; or ( 3 ) indices of bone and mineral metabolism : calcium , phosphate , bone alkaline phosphatase , osteocalcin , propeptides of procollagen type 1 and 3 , and bone mineral content . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The most promising potential management agent is recombinant human growth hormone , which can stimulate bone formation via production of insulin like growth factor 1 ( IGF 1 ) and its associated binding protein , IGFBP 3 , which correlates with bone mineral density in adults . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that large doses of rHGH were ineffective in improving disordered bone formation despite increasing serum IGF 1 and IGFBP 3 . ^^^ The rHGH independent rise in serum levels of the inhibitory binding protein IGFBP 4 suggests a mechanism by which improved bone formation is prevented despite successful elevation of IGF 1 and IGFBP 3 in the burned child . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although levels of IGF 1 and IGFBP 3 did not differ , the elevation of IGFBP 1 levels in FHA resulted in a reduced ( P < 0 . 01 ) ratio of IGF I / IGFBP 1 , which may decrease the bioactivity and hypoglycemic effect of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Long term effects of insulin like growth factor ( IGF ) 1 on serum IGF 1 , IGF binding protein 3 and acid labile subunit in Laron syndrome patients with normal growth hormone binding protein . ^^^ During IGF 1 treatment , the IGFBP 3 concentrations in the girl gradually increased , whereas in the boys there was a 60 % decrease during the first week , followed by gradual increase towards baseline . ^^^ We conclude that IGF 1 treatment induces and initial suppression and then an increase in the IGFBP 3 and ALS concentrations , confirming data from animal experiments that IGFBP 3 synthesis is not solely under GH control . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Basal GH , IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) plasma levels and the IGF I / IGFBP 3 molar ratio remained unaffected by NTX . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , in dominant and subordinate follicles were measured to determine if changes in IGF or IGFBP gene expression are associated with follicular dominance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , we showed that conditions mimicking hyperinsulinemia , rather than high levels of IGFs , could regulate BREC growth and that the IGF 1 analog , Des ( 1 3 ) , even with reduced affinity for IGFBPs but in part capable of binding to IGFBP 3 , significantly stimulated BRECs growth only at 10 nmol / L . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of both insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 increased significantly in the GH treated group ( p < 0 . 008 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Reduction of ventral prostate weight by finasteride is associated with suppression of insulin like growth factor 1 ( IGF 1 ) and IGF 1 receptor genes and with an increase in IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Short term treatment studies have shown that GHRH and the GHRP ' s can enhance GH secretion and elevate IGF 1 and IGFBP 3 levels ; that GHRH may promote sleep ; and that these agents are generally well tolerated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , human bone marrow cultures demonstrate components of the skeletal IGF regulatory system : IGF 1 , IGF binding proteins , and evidence of IGFBP 3 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In aging , there are marked decreases in IGF 1 and 2 , normal levels of IGFBP 3 , and marked increases in IGFBP 1 in serum . ^^^ In these conditions , serum levels of IGF 1 and 2 are markedly diminished , IGFBP 3 levels are also decreased , and IGFBP 1 levels are markedly increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Elucidation of novel pathways downstream of p 53 have established a link between this important tumor suppressor gene and the insulin like growth factor 1 receptor ( IGF 1r ) , either via direct regulation of IGF 1 receptor levels , or modulation of IGFs via transactivation of the insulin like growth factor binding protein 3 ( IGF BP 3 ) gene . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lysis of platelets releases the contents of the alpha granules , which contain growth factors , including insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) . ^^^ We investigated the mechanism by which IGF 1 and IGFBP 3 appeared in the alpha granules with a goal of modulating their levels in platelets to affect platelet functions . ^^^ Reverse transcription PCR was initially used to test whether megakaryocytes contained IGFBP 3 and IGF 1 messenger RNA transcripts . ^^^ We found that megakaryocytes did not express the IGFBP 3 gene , but did have IGF 1 messenger RNA . ^^^ We subsequently investigated whether they incorporated IGFBP 3 and IGF 1 by the process of endocytosis and packaged them into the alpha granules . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our results demonstrate that exogenous pGH increases circulating IGF 1 and IGFBP 3 concentrations and visceral organ growth in neonatal pigs , suggesting that the somatotrophic axis is functional in the neonate . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Circulating concentrations of leptin , IGF 1 , IGF 2 , and insulin like growth factor binding protein 3 ( IGFBP 3 ) were measured in a population based sample of 50 to 80 year old men ( n=217 ) and women ( n=198 ) by specific RIA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To this end , whole ovarian dispersates from immature rats were cultured for 48 h in the absence or presence of IGF 1 or IGF binding protein 3 ( IGFBP 3 ) , with or without IL 1beta . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Reduced pulsatile fraction of TSH , GH , and PRL secretion and low serum concentrations of T 4 , T 3 , insulin growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) were documented in the untreated state . ^^^ Pulsatile GH secretion was amplified > 6 and > 10 fold , respectively , by GHRP 2 and GHRH + GHRP 2 infusions , generating mean increases of serum IGF 1 ( 66 % and 106 % ) , IGFBP 3 ( 50 % and 56 % ) , and ALS ( 65 % and 97 % ) within 45 h . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that 2 month treatment with MK 677 in healthy obese males caused a sustained increase in serum levels of GH , IGF 1 , and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline GH secretion , GH binding protein ( BP ) , insulin like growth factor 1 ( IGF 1 ) , and IGFBP 3 levels were at the lower limit of normal . ^^^ The rhGH treatment increased IGF 1 and IGFBP 3 plasma levels to above normal values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As hypercholesterolemia is common in GH deficient adults , and because there is experimental evidence that GH may play a role in regulating plasma cholesterol , we decided to investigate the activity of the GH IGF axis in an elderly population by measuring serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) levels and to study their relationship with blood lipid levels . ^^^ Men had significantly lower levels of IGFBP 3 , high density lipoprotein cholesterol ( HDL C ) and apoprotein A 1 ( ApoA 1 ) compared to the women , whereas IGF 1 and IGF 2 were only slightly lower . ^^^ Using linear regression analysis , we observed an inverse relationship of age with IGF 1 ( r = 0 . 35 ; P < 0 . 001 ) , IGF 2 ( r = 0 . 40 ; P < 0 . 001 ) , IGFBP 3 ( r = 0 . 52 ; P < 0 . 001 ) , body mass index , and lipid levels . ^^^ Univariate regression analysis showed a strong and positive correlation of both IGF 1 and IGFBP 3 with HDL C and ApoA 1 . ^^^ Furthermore , a strong association was documented among IGF 1 , IGF 2 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Administration of recombinant human GH at the dose of 2 . 0 IU / kg for 3 consecutive days induced a minimal response of serum total IGF 1 and IGF binding protein 3 ( IGFBP 3 ) , suggesting partial GH resistance . ^^^ This sustained the serum total IGF 1 level , but not the serum IGFBP 3 level , within the normal range . ^^^ These findings suggest 1 ) that this leprechaun patient has an IGF 1 deficient state and partial GH resistance , as reflected by impaired production of IGF 1 and IGFBP 3 ; 2 ) that rhIGF 1 treatment works effectively for preventing postnatal growth retardation and normalizing glucose metabolism in patients with extreme insulin resistance ; 3 ) that this treatment requires relatively higher dose of rhIGF 1 ; and 4 ) that treatment appears to be safe and devoid of adverse effects . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGF 2 were quantified by radioimmunoassay ( RIA ) , IGFBP 3 by immunoradiometric assay ( IRMA ) , and changes in IGFBP 1 , 2 , 3 , and 4 were estimated by Western ligand blotting ( WLB ) . ^^^ The decreases observed in IGF 1 , IGF 2 , and IGFBP 3 in patients with hepatic failure and their subsequent restoration after LT probably result primarily from the reduced number of functional hepatocytes in ESLD and their subsequent replacement by healthy hepatic tissue . ^^^ We conclude that LT in patients with severe hepatic insufficiency enhances the potential for normal cell growth and replication by restoring serum IGF 1 , IGF 2 , and IGFBP 3 concentrations to normal concomitantly with the improvement in hormonal and nutritional status . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated 29 prepubertal healthy children ( 13 girls / 16 boys ; mean age 9 . 3 y to study intraindividual variation in serum GHBP and to explore whether any such variation was related to changes in IGF 1 , IGF binding protein 3 ( IGFBP 3 ) or urinary excretion of GH . ^^^ Blood samples were taken every month for 1 y , for measurements of GHBP , IGF 1 , and IGFBP 3 . ^^^ The mean GHBP concentration during the year correlated with height velocity ( rs = 0 . 37 , p < 0 . 05 ) and the mean serum concentration of IGF 1 ( rs = 0 . 42 , p < 0 . 05 ) and IGFBP 3 ( rs = 0 . 60 , p < 0 . 001 ) . ^^^ The mean GHBP concentration during the year is correlated with the mean serum concentrations of IGF 1 , IGFBP 3 , and with height velocity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , glucose , insulin , HbAlc and serum free fatty acids ( FFA ) levels were analysed repeatedly . ^^^ IGF 1 and IGFBP 3 plasma levels were identical in both groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The Objective of this paper is to determine the relation between fetal macrosomia in offspring of nondiabetic women , and the levels of insulin like growth factors ( IGF 1 , IGF 2 ) , insulin growth factor binding protein 3 ( IGFBP 3 ) and insulin , in maternal and neonatal compartments . ^^^ Maternal and neonatal serum samples were analyzed for levels of IGF 1 , IGF 2 , IGFBP 3 and insulin , by specific radioimmunoassays ( RIAs ) . ^^^ Fetal cord blood levels of IGF 1 and IGFBP 3 are directly correlated with the birth weight of large for gestational age fetuses . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH , glucagon , IGF 1 , IGFBP 3 levels , were significantly lowered by octreotide in both type 1 and type 2 diabetic patients whereas cortisol and epinephrine remained unmodified . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of our study was to determine the serum levels of IGF 1 , IGF 2 , and their binding proteins IGFBP 1 and IGFBP 3 in young children 3 months to 2 years of age with nonorganic failure to thrive ( NOFTT ) . 29 children with NOFTT and 32 normal controls were recruited and studied for auxologic and hormonal parameters . ^^^ IGF 1 and IGF 2 serum levels were highly correlated to serum IGFBP 3 but there were no differences in the serum IGF 1 , IGF 2 and IGFBP 3 levels between the groups . ^^^ We conclude that IGF 1 , IGF 2 , and IGFBP 3 are not useful for the evaluation of nutritional status in children , under the age of 2 years , with NOFTT . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Long [ R 3 ] insulin like growth factor 1 reduces growth , plasma growth hormone , IGF binding protein 3 and endogenous IGF 1 concentrations in pigs . ^^^ A four day infusion of Long [ R 3 ] IGF 1 ( LR3IGF 1 ; 180 micrograms / kg / day ) decreased the average daily gain , food intake , and plasma IGFBP 3 , IGF 1 and insulin concentrations . ^^^ Co administration of pGH ( 30 micrograms / kg / day ) with LR3IGF 1 had no interactive effect on growth performance , and plasma insulin , IGFBP 3 and IGF 1 concentrations remained suppressed . ^^^ Infusion of IGF 1 ( 180 micrograms / kg / day ) decreased plasma insulin and mean GH concentrations but had no significant effect on IGFBP 3 concentrations . ^^^ A combination of IGF 1 and pGH injection ( 30 micrograms / kg / day ) increased plasma IGFBP 3 concentrations but plasma insulin levels remained suppressed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Analysis of the distribution of IGF 1 , IGF 2 and IGFBP 3 in fluid from healthy and atretic follicles revealed that the majority of these growth factors ( > 80 % of total IGF 2 ) were in the 150KDa complex , indicating that the ALS present was functional , in that it formed the ternary complex with a molecule of IGFBP 3 and IGF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These results indicate that the decrease in GH gene expression and secretion , as well as the expression of genes induced by GH such as IGF 1 and IGFBP 3 , is due to aging and not to the increase in body weight that takes place with aging . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of octreotide on growth hormone , IGF 1 , IGFBP 3 , glucagon , cortisol and epinephrine response to insulin induced hypoglycaemia in insulin dependent diabetic patients . ^^^ Although octreotide suppressed both GH ( 0 . 5 + / 0 . 01 vs 9 . 5 + / 0 . 9 ng / ml , p < 0 . 001 ) and glucagon ( 110 + / 9 vs 165 + / 10 pg / ml , p < 0 . 05 ) responses , it did not affect cortisol , epinephrine , IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As observed for IGFBP 3 , ternary complexes containing IGFBP 5 form preferentially in the presence of IGF 1 , even though IGFBP 5 has a preferential affinity for IGF 2 over IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effect of an intravenous infusion of IGF 1 and insulin on IGFBP 1 , IGFBP 3 , acid labile subunit , free and bound IGF 1 , catecholamines and potassium in normal volunteers during an amino acid and glucose clamp . ^^^ MEASUREMENTS : Circulating levels of total IGF 1 , free and bound IGF 1 , Insulin , IGFBP 1 , IGFBP 3 , ALS , catecholamines and potassium were measured . ^^^ There was no significant change in IGFBP 3 or ALS during the IGF 1 or insulin infusions . ^^^ IGF 1 and insulin under conditions of adequate substrate supply have acute effects on IGFBP 1 and potassium physiology , but have little effect on IGFBP 3 , ALS or catecholamines . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since insulin like growth factor 1 ( IGF 1 ) is an important hormone regulator of intestinal proliferation , this study examined the effects of dietary protein content and tumor necrosis factor ( TNF ) on mRNA levels of IGF 1 , IGF 1 receptor , IGF binding protein 3 ( IGFBP 3 ) , and IGFBP 4 and on the histology of the colon , jejunum , and ileum in protein malnourished rats . ^^^ However , in the jejunum and ileum , refeeding had no effect but TNF caused a decrease in IGF 1 and IGFBP 3 mRNA levels in malnourished rats . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The basal levels of insulin like growth factor 1 ( IGF 1 ) tended to be higher before the operation than afterwards and those of its binding protein 3 ( IGFBP 3 ) were significantly so ( P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 correlated with IGFBP 3 ( r = +0 . 7 , p < 0 . 001 ) . ^^^ Whilst IGF 1 and IGFBP 3 fell , daytime growth hormone increased from 3 . 3+ / 0 . 6 to 6 . 7+ / 0 . 8 mU l ( 1 ) ( p=0 . 01 ) , and cortisol from 197+ / 48 to 594+ / 98 nmol l ( 1 ) ( p = 0 . 005 ) . ^^^ Increasing protein breakdown was related to falling IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to compare measurements of urinary growth hormone ( GH ) , serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) between two groups of post menarcheal girls , 13 18 y of age , one comprising 64 type 1 diabetic patients and the other 64 healthy girls matched for age and stage of puberty . ^^^ Significantly higher urinary GH concentrations but lower IGF 1 and IGFBP 3 levels were found in diabetic girls than in controls ( p < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Both vitamin D analogues inhibited IGF 1 stimulated growth of MCF 7 cells and enhanced the production of IGFBP 3 as determined by Western ligand blotting . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In summary : ( 1 ) calcium depletion in weanling rats increased several serum IGFBPs without an effect on IGF 1 ; and ( 2 ) calcium repletion induced an acute reduction in serum IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
By studying the basis of diabetic abnormalities relating to the growth hormone ( GH ) , the insulin like growth factor ( IGF ) dynamics in this patient , i . e . reductions of GH , IGF 1 , IGF 2 , IGF Binding protein ( IGF BP ) 2 and IGF BP 3 , were detected . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Studies in transgenic mice overexpressing IGF 1 , des ( 1 3 ) IGF 1 , or IGFBP 3 show that IGF 1 causes ductal hypertrophy in the lactating mouse and prevention of post lactational mammary gland involution . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have shown this to be true with a diverse sample of eukaryotic proteins : IGF 1 , IGFBP 3 , 3C proteinase , TGF beta 2 , sTGF beta RII , BDNF , GDNF , mEGFBP , leptin , and GFP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 which inhibits IGF action decreased neither basal nor Ca stimulated but IGF 1 stimulated NadPi transport and DNA synthesis , indicating that Ca stimulated NadPi transport and DNA synthesis independently of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin also reduced intrafollicular concentrations of IGF 1 at days 3 and 5 after weaning ( treatment by day interaction ; P < 0 . 02 ) while the amounts of IGFBP 3 and IGFBPs of molecular mass 30 and 22 kDa decreased from day 3 to day 5 in saline treated animals only ( treatment by day interaction ; P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The dP and adP IGF 1 and IGF BP 3 levels were greater than preLT levels ( p < 0 . 001 ) ; no differences were found between preLT , dP , and adP IGF BP 1 levels . ^^^ IGF 1 and IGF BP 3 increment on dP ( and sustained on adP ) is possibly due to liver regeneration , in contrast with inhibition of body growth on dP , possibly due to central and peripheral effects of corticosteroid . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although IGF 1 is potently antiapoptotic for neurons and oligodendrocytes , marked gliosis and IGFBP 3 expression are seen , similar to in vivo injury responses , where intraventricular injection of IGF 1 postinjury enhances neuronal survival by blocking apoptosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH and IGF 1 basal concentrations were significantly higher in pre menopausal than in post menopausal women , while IGF 2 , IGFBP 1 and IGFBP 3 concentrations did not vary significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) play an important role in regulating follicle growth and maturation . ^^^ We have evaluated whether responsiveness to gonadotrophins during an in vitro fertilization ( IVF ) treatment is related to follicular fluid IGF 1 and IGFBP 3 concentrations . ^^^ We also investigated if a difference is present in IGF 1 and IGFBP 3 concentrations between patients treated with human menopausal gonadotrophin ( HMG ) and patients treated with highly purified follicle stimulating hormone ( FSH ) . ^^^ We have measured IGF 1 and IGFBP 3 in follicular fluid from pre ovulatory follicles in an IVF programme . ^^^ IGFBP 3 concentrations were not correlated with any other parameter measured nor were IGF 1 and IGFBP 3 concentrations correlated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Irreversible increase of serum IGF 1 and IGFBP 3 levels in GnRH dependent precocious puberty of different etiologies : implications for the onset of puberty . ^^^ In normal puberty , as well as in precocious puberty , serum GH , IGF 1 and IGFBP 3 are increased as a consequence of the increase in sex hormone secretion . ^^^ Normal values for serum IGF 1 and serum IGFBP 3 were established in our laboratory from a population of 165 clinically controlled subjects , aged 0 . 5 15 years . ^^^ In Gr 1 , serum IGF 1 SDS for chronological age ( CA ) , but not IGFBP 3 SDS CA , was significantly high before GnRH analog treatment ( mean+ / SD 1 . 33+ / 1 . 84 and 0 . 68+ / 1 . 55 , p < 0 . 05 and p = NS , respectively ) . ^^^ IGF 1 SDS CA remained high and IGFBP 3 SDS CA remained normal during treatment ( 1 . 34+ / 2 . 0 and 0 . 73+ / 1 . 93 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , estradiol and progesterone were measured by radioimmunoassay ( RIA ) in each sample . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For this study we selected operated patients who were biochemically in remission , defined by normal circulating IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) concentrations , normal glucose suppressed plasma GH concentration ( < 0 . 38 microg / l ) , and the normalization of the paradoxical rise of GH to TRH or GnRH . ^^^ ApEn values in treated and untreated patients correlated significantly with the plasma concentration of IGF 1 ( r=0 . 531 ) and IGFBP 3 ( r=0 . 598 ) , and the log transformed 24h GH secretion rate ( r=0 . 749 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH response to provocation and circulating IGF 1 and IGF binding protein 3 concentrations , the IGF 1 generation test and clinical response to GH therapy in children with beta thalassaemia . ^^^ We studied the GH response to provocation by clonidine and glucagon , measured the circulating concentrations of insulin , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and ferritin , and evaluated IGF 1 generation after a single dose of GH ( 0 . 1 mg / kg per dose ) in 15 prepubertal patients with thalassaemia , 15 age matched children with constitutional short stature ( CSS ) ( height standard deviation score less than 2 , with normal GH response to provocation ) and 11 children with isolated GH deficiency ( GHD ) . ^^^ They had significantly decreased circulating concentrations of IGF 1 and IGFBP 3 ( 47 . 5 + / 19 ng / ml and 1 . 2 + / 0 . 27 mg / l respectively ) compared with those with CSS ( 153 + / 42 ng / ml and 2 . 06 + / 0 . 37 mg / l respectively ) , but the IGF 1 and IGFBP 3 concentrations were not different from those with GHD ( 56 + / 25 ng / ml and 1 . 1 + / 0 . 32 mg / l respectively ) . ^^^ These data demonstrate that the GH IGF 1 IGFBP 3 axis in thalassaemic children is defective . ^^^ Serum ferritin concentration correlated significantly with GH peak response to provocation ( r = 0 . 36 , P < 0 . 05 ) and circulating IGF 1 ( r = 0 . 47 , P < 0 . 01 ) and IGFBP 3 ( r = 0 . 42 , P < 0 . 01 ) concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To evaluate the role of the GH IGF 1 axis during the growth deceleration , serum IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and an arginine stimulation test were performed at 1 , 3 or 6 months after GH withdrawal , and compared with pretreatment response . ^^^ Serum IGF 1 and IGFBP 3 levels were normal throughout . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The major cell associated binding site for [ 125I ] IGF 1 is IGFBP 3 and for [ 125I ] IGF 2 are IGFBP 3 and IGFBP 5 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Northern blots were performed using total RNA hybridized with 32P labeled cDNA probes ( human IGF 1 and human IGFI R ) and cRNA probes ( rat IGF 2 , human IGFII R , human IGFBP 1 , pig IGFBP 3 , and pig GHR ) . ^^^ Messenger RNA levels of IGFI R , IGFBP 3 , and GHR and concentrations of IGFBP 1 and 2 were abundant during fetal and early postnatal life . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These data suggest that the compensatory hyperinsulinemia that characterizes adolescent obesity chronically suppresses levels of IGFBP 1 , and low IGFBP 1 concentrations may serve to increase the bioavailability of free IGF 1 , which may , in turn , contribute to lower circulating GH , total IGF 1 , and IGFBP 3 concentrations . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGF binding protein 3 did not change significantly over the 20 week period ( P = 0 . 24 and P = 0 . 74 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 levels were increased during the PO phase ( 253 + / 20 mg / L ) compared to those during the EF phase ( 210 + / 16 mg / L ; P = 0 . 03 ) , whereas serum IGF binding protein 3 , IGF 2 , and GH binding protein were similar during the two phases . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We attempted to analyze the relationship between the bone mineral density ( [ BMD ] measured by dual photon absorptiometry ) and auxanologic parameters , degree of siderosis , function of the growth hormone ( GH ) / insulin like growth factor 1 ( IGF 1 ) / IGF binding protein 3 ( IGFBP 3 ) axis , calcium phosphate balance , parathyroid hormone ( PTH ) , and cytokines ( interleukin 1beta [ IL 1 ] and tumor necrosis factor alpha [ TNF alpha ] ) in 30 prepubertal children with beta thalassemia major and 15 age matched children with constitutional short stature ( CSS ) , who have normal glucose tolerance and thyroid function . ^^^ Thalassemic patients had significantly lower circulating concentrations of IGF 1 and IGFBP 3 ( 49+ / 21 ng / mL and 1 . 2+ / 0 . 25 mg / L , respectively ) compared with control children ( 153+ / 42 ng / mL and 2 . 1+ / 0 . 37 mg / L , respectively ) . ^^^ BMD was highly correlated with the circulating concentrations of IGF 1 and IGFBP 3 , as well as with the auxanologic parameters ( age , weight , height , height standard deviation score [ HSDS ] , and body mass index [ BMI ] ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and 2 in sera from patients with HCAO were carried , as in the case of sera from control subjects , bound to IGFBP 3 in the approximately 150 kD complex , which is retained in the circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating IGFBP 3 originates mainly in hepatic nonparenchymal cells , where IGF 1 increases IGFBP 3 mRNA stability , but insulin increases IGFBP 3 gene transcription . ^^^ Studies of mechanisms underlying the modulation of IGF 1 and IGFBP 3 gene transcription , and identification of critical nuclear proteins involved , should lead to new understanding of the role and regulation of these important growth factors in diabetes mellitus and other metabolic disorders . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The hormone injections increased the serum concentration of IGF 1 ( from 72 . 7 + / 40 . 9 to 483 . 7 + / 251 . 4 eta g / ml , p = . 001 ) and IGFBP 3 ( from 1 . 82 + / 0 . 66 to 2 . 72 + / 1 . 18 mg / L , p = . 012 ) , and decreased serum albumin ( from 34 . 3 + / 5 . 5 to 31 . 4 + / 4 . 6 g / L , p = . 009 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The complex of IGF 1 and its specific binding protein , IGFBP 3 , may stimulate the osteoblastic activities via prolonged serum half life and increased cellular association of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of recombinant human IGF 1 administration on concentrations of acid labile subunit , IGF binding protein 3 , IGF 1 , IGF 2 and proteolysis of IGF binding protein 3 in adolescents with insulin dependent diabetes mellitus . ^^^ We have therefore studied the effects of a single subcutaneous dose of rhIGF 1 ( 40 micrograms / kg at 1800 h ) , when compared with an untreated control night , in 17 subjects with IDDM , on serum concentrations of IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and IGFBP 3 proteolysis . ^^^ On the baseline night , IGFBP 3 levels correlated with the sum of IGF 1 and IGF 2 ( r = 0 . 73 , P = 0 . 02 ) and with levels of the ALS ( r = 0 . 7 , P = 0 . 002 ) . ^^^ However after rhIGF 1 , the sum of IGF 1 and IGF 2 no longer correlated with IGFBP 3 , whereas the relationship with ALS was maintained . ^^^ This can not be explained by alterations in IGFBP 3 proteolysis , and may relate to the relative stability of ALS / IGFBP 3 when complexed principally with IGF 1 rather than IGF II . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were measured in 90 neonates during the first 5 days of life . ^^^ IGF 1 and IGFBP 3 correlated with gestational age in AGA but not in SGA neonates and there was no correlation with birth weight in either group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , testosterone , dihydrotestosterone ( DHT ) , 3 alpha androstanediol ( 3 alpha diol ) , IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 concentrations were evaluated in human benign prostatic hyperplasia ( BPH ) tissue . ^^^ IGF 1 , IGFBP 2 , and IGFBP 3 were measured by radioimmunoassay , and IGF 2 by immunoradio metric assay , after acidification and chromatography on Sep pak C 18 Cartridges for IGF 1 and IGF 2 . ^^^ IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels were 24 + / 3 . 7 , 121 + / 14 ng / g tissue and 0 . 44 + / 0 . 05 and 1 . 2 + / 0 . 17 micrograms / g tissue , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH treatment significantly increased serum levels of total IGF 1 from 98 ( 68 ) to 323 ( 126 ) microg / l , free IGF 1 from 0 . 48 ( 0 . 47 ) to 2 . 24 ( 1 . 66 ) microg / l , IGFBP 3 from 1 , 874 ( 1 , 178 ) to 3 , 520 ( 778 ) microg / l and ALS levels from 9 , 182 ( 5 , 524 ) to 16 , 872 ( 6 , 278 ) microg / l ( all p < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thyroid hormones have been shown to be involved in the regulation of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) expression . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) but not insulin like growth factor 1 ( IGF 1 ) remains elevated in euthyroid TSH suppressed Graves ' disease . ^^^ This is a cross sectional study to look at the effects of thyroid hormone status on the circulating levels of IGF 1 and IGFBP 3 in a group of 127 patients , aged 20 80 years , who were hyperthyroid , hypothyroid , rendered euthyroid and clinically euthyroid with normal free thyroxine ( fT 4 ) , but suppressed thyroid stimulating hormone ( TSH ) levels . ^^^ TSH was measured by the IMx ( Abbott ) ultrasensitive assay , while radioimmunoassays for total T 3 and T 4 were performed using kits from ICN , USA ; fT 4 and fT 3 using kits from DPC USA ; IGF 1 and IGFBP 3 using kits from Nichols Institute Diagnostics B . ^^^ Hence , whilst low IGF 1 is associated with hypothyroidism , high IGFBP 3 is associated with hyperthyroidism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total IGF 1 ( 129+ / 10 compared with 124+ / 11 ng / ml , P=0 . 72 ) and IGF binding protein 3 ( 2854+ / 94 compared with 2623+ / 128ng / ml , P=0 . 16 ) , were similarly depressed compared with young adult norms in both groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : IUGR newborns showed significantly greater concentrations of IGFBP 1 ( P < 0 . 0001 ) and lower concentrations of IGF 1 ( P < 0 . 0001 ) and IGFBP 3 ( P < 0 . 0001 ) than did controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Prostate epithelial cell proliferation is regulated by insulin like growth factor 1 ( IGF 1 ) which is mitogenic and anti apoptotic , and IGF binding protein 3 ( IGFBP 3 ) which is an apoptotic agent in these cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Developmental changes in the GH insulin like growth factor 1 ( IGF 1 ) axis were evaluated in female rhesus monkeys to test the hypothesis that estradiol differentially regulates IGF 1 secretion and molar ratios of IGF 1 to IGF binding protein 3 ( IGFBP 3 ) from adolescence into adulthood and that estradiol can reestablish GH secretion in the face of enhanced IGF 1 negative feedback inhibition of GH . ^^^ Serum IGF 1 and IGFBP 3 , in the absence of estradiol replacement , increased significantly throughout puberty before declining from late adolescence into adulthood . ^^^ Supplementation with IGF 1 resulted in a significant increase in both serum IGF 1 and IGFBP 3 concentrations at all ages , although the effect was less in juvenile females . ^^^ Nevertheless , the age dependent increase and decline in IGF 1 and IGFBP 3 were maintained in these supplemented animals . ^^^ Estradiol replacement significantly increased both serum IGF 1 and IGFBP 3 through adolescence , even in IGF 1 supplemented animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of fenretinide on plasma IGF 1 and IGFBP 3 in early breast cancer patients . ^^^ IGF 1 and IGFBP 3 were measured on plasma samples obtained at randomization and after an interval of approximately 1 year , from 39 and 33 stage 1 breast cancer patients assigned to receive 4 HPR , and from 39 and 34 untreated controls , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and insulin were determined after 4 weeks of dietary treatment by radioimmunoassay while IGFBP 3 was determined by an immunoradiometric assay . ^^^ Serum levels of IGF 1 and IGFBP 3 were higher ( p < 0 . 05 ) in rhGH treated rats fed at 60 % of ad libitum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The hormonal assessment included repeated blood samples for estimation of IGF 1 , IGFBP 3 and repeated OGTTs . ^^^ In each case 100 sigma g octreotide ( Sandostatin lambda , Sandoz , Basel ) was injected to test the acute response of GH , IGF 1 and IGFBP 3 . ^^^ Despite these observations a strong correlation of IGF 1 and IGFBP 3 did not exist before one year post operatively either in the cured or in the non cured patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured serum levels of free IGF 1 and free IGF 2 , total IGF 1 , total IGF 2 , big IGF 2 and IGFBP 1 , IGFBP 2 and IGFBP 3 in patients with NICTH before ( n=14 ) and after surgical removal of the tumour ( n=3 ) . ^^^ In accordance with earlier observations levels of total IGF 1 , total IGF 2 , and IGFBP 3 were decreased , whereas IGFBP 1 and IGFBP 2 were increased in NICTH ( all p values < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our aim was to study the prognostic value of growth hormone ( GH ) stimulated insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) generation in patients with compensated [ group 1 ( N = 8 ) with a Child Pugh ( CP ) score of 5 8 ] and decompensated postnecrotic liver cirrhosis [ group 2 ( N = 7 ) with a CP score of 9 12 ] . ^^^ Serum levels of IGF 1 , GH binding protein ( GHBP ) , and IGFBP 3 were measured before and 24 hr after a single subcutaneous injection of recombinant human GH ( rhGH , 0 . 14 units / kg ) . ^^^ Baseline IGF 1 ( 9 . 98 + / 2 . 0 vs 6 . 38 + / 0 . 8 nmol / liter ) , GHBP ( 9 . 2 + / 3 vs 5 . 7 + / 0 . 8 % / 50 microl ) , and IGFBP 3 serum levels at baseline ( 1 . 7 + / 0 . 3 vs 0 . 86 + / 0 . 2 mg / liter ) and at 24 hr ( 2 . 04 + / 0 . 38 vs 0 . 99 + / 0 . 3 mg / liter ) did not add to the predictive value of stimulated IGF 1 levels at 24 hr and were less accurate in predicting the outcome in comparison to CP score ( 80 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our goal was to determine whether an association exists between serum levels of IGF 1 and one of its binding proteins , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and prostate cancer risk . ^^^ METHODS : An immunoradiometric assay was used to quantify IGF 1 levels and IGFBP 3 levels in serum samples as part of a population based , case control study in Sweden . ^^^ RESULTS : The mean serum IGF 1 level for case patients ( 158 . 4 ng / mL ) was significantly higher than that for control subjects ( 147 . 4 ng / mL ) ( P = . 02 ) ; corresponding mean serum IGFBP 3 levels were not significantly different between case patients ( 2668 ng / mL ) and control subjects ( 2518 ng / mL ) ( P = . 09 ) . ^^^ Serum IGFBP 3 levels were not significantly associated with increased risk of disease , and adjustment for IGFBP 3 had little effect on the association between IGF 1 levels and risk of prostate cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum free IGF 1 , total IGF 1 , IGFBP 1 and IGFBP 3 levels in an elderly population : relation to age and sex steroid levels . ^^^ MEASUREMENTS : In a cross sectional study of 218 healthy people ( 103 men , 115 women ) aged 55 80 years we measured serum total and free IGF 1 , IGFBP 1 and IGFBP 3 levels and sex steroids . ^^^ Total IGF 1 and IGFBP 3 decreased with age ( r = 0 . 20 , P = 0 . 005 and r = 0 . 24 , P = 0 . 001 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Serum IGF 1 and IGFBP 3 were significantly lower in GHDAs , but a considerable overlap existed ( IGF 1 ( microgram / l ) 87 + / 12 ( GHDA ) vs 177 + / 10 ( Control ) ( P < 0 . 001 ) ) . ^^^ The molar IGF 1 : IGFBP 3 ratio was significantly lower in GHDAs ( 0 . 18 + / 0 . 01 vs 0 . 23 + / 0 . 02 ( P = 0 . 002 ) ) . ^^^ IGF 1 correlated negatively with age in CON but not in GHDAs , whereas IGF 1 correlated positively with IGFBP 3 only in GHDAs . ^^^ Multiple regression analysis revealed that age and IGFBP 1 were the only significant predictors of IGF 1 in CON , whereas IGFBP 3 and , to a lesser extent TBF , were the only independent predictors of IGF 1 in GHDAs . ^^^ CONCLUSIONS : 1 ) IGF 1 levels are regulated by several variables in addition to GH status 2 ) age per se is an independent negative determinant in healthy subjects but not in GHDA 3 ) it is probable that some cases of paradoxically high IGF 1 levels in GHDA are secondary to inappropriately elevated IGFBP 3 levels . 4 ) in mid adulthood males have higher IGF 1 levels than females and it is likely that testosterone directly stimulates IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Serum levels of insulin , IGF 1 and IGFBP 3 in obese children did not differ from those in controls . ^^^ No relationship was found between the serum levels of insulin and IGF 1 , IGFBP 1 , or IGFBP 3 in obese subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously shown that the major correlates of growth following growth hormone ( GH ) therapy in growth hormone deficient ( GHD ) children are changes in circulating insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , suggesting a synergistic interaction between IGF 1 and IGFBP 3 ( 1 ) . ^^^ The effect of GH therapy on the immunoreactive forms and distribution of IGFBP 3 , IGF 1 , the acid labile subunit , and growth rate in GH deficient children . ^^^ The second aim of this project was to assess the changes in distribution of the immunoreactive forms of IGFBP 3 and IGF 1 among the ternary ( ALS / IGFBP 3 / IGF ) complex , the binary ( IGFBP 3 / IGF ) complex , and uncomplexed IGF during the first year of GH therapy , and to explore further the correlation with growth response to GH . ^^^ Plasma samples , prior to therapy and after the first year of GH treatment , were separated by neutral size exclusion chromatography and then subjected to IGFBP 3 immunoradiometric assay ( IRMA ) , IGFBP 3 WIB , and IGF 1 IRMA analysis . ^^^ Growth rate , assessed by the height velocity SDS for bone age , correlated best with the changes in ternary complex IGFBP 3 ( r = 0 . 72 , p = 0 . 01 ) and ternary complex IGF 1 ( r = 0 . 56 , p = 0 . 10 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied 11 elderly male ( 50 78 years ) marathon runners and 10 age matched male ( 52 73 years ) sedentary controls to evaluate plasma concentrations of GH , total and free IGF 1 and IGF 2 and of IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 and insulin . ^^^ In conclusion , our findings do not support the hypothesis that the age associated decline in GH , IGF 1 and IGFBP 3 may be preventable by intensive endurance training . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The amounts of IGFBP 3 in conditioned media were relatively low under basal conditions when analyzed by ligand blotting with 125I IGF 2 , but were increased dramatically relative to serum free controls by exposure to IGF 1 ( 100 ng / ml ) or IGF 2 ( 100 ng / ml ) for 24 h . ^^^ These increases in IGFBP 3 protein corresponded with dose dependent increases in IGFBP 3 mRNA , with IGF 2 eliciting a smaller response than was elicited by IGF 1 at each concentration . ^^^ Leu IGF 1 , which has reduced affinity for the IGF 1 receptor but normal affinity for IGFBPs , failed to increase IGFBP 3 protein and mRNA levels , whereas B chain IGF 1 ( normal affinity for the receptor but reduced affinity for IGFBPs ) elicited the response , thus implying an IGF 1 receptor mediated event . ^^^ Time course studies indicated that IGFBP 3 mRNA was increased fourfold by 3 h of IGF 1 treatment , with maximal increases of eightfold above serum free controls observed between 8 and 13 h of treatment . ^^^ By 24 h of treatment , IGFBP 3 mRNA levels had declined and were approximately threefold above controls in cells exposed to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Monthly fasting 23 hours postdose levels of serum DHEA , DHEAS , T , lipoproteins , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and liver function tests . ^^^ At 3 months , the ratio of IGF 1 to IGFBP 3 rose by 36 . 1 % + / 12 . 7 % , but it fell to placebo values by 6 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the effects of hMG and highly purified FSH on follicular production of the ovarian growth factors transforming growth factor beta 1 ( TGF beta 1 ) , insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) , and insulin like growth factor binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate serum and follicular fluid insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) behavior in spontaneous and in superstimulated cycles . ^^^ DESIGN : Estradiol , GH , IGF 1 , and IGFBP 3 were evaluated in serum and in follicular fluid during spontaneous and stimulated cycles . ^^^ MAIN OUTCOME MEASURE ( S ) : Growth hormone , IGF 1 , and IGFBP 3 were assayed by RIA and immunoradiometric assay techniques . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , serum IGF 1 was neither correlated with bone turnover nor with bone volume and was only weakly associated with bone matrix IGF 1 when adjusted for the serum concentration of IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGFBP 3 were also measured , and stadiometer height measurements were recorded . ^^^ Serum levels of IGF 1 and of IGFBP 3 did not increase . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Free IGF 1 and IGF 2 were measured by ultrafiltration of serum , and IGFBP 3 proteolytic activity was determined by a [ 125I ] recombinant human IGFBP 3 degradation assay . ^^^ The relative elevations of free IGF 1 levels despite decreased total IGF 1 levels could thus relate to augmented IGFBP 3 proteolysis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Urinary growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 measurements in the diagnosis of adult GH deficiency . ^^^ These data demonstrate that urinary GH and urinary and serum IGF 1 and IGFBP 3 are not suitable diagnostic markers for GHD in elderly subjects . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although there was a strong correlation between IGF 1 and IGFBP 3 ( r = 0 . 559 , p < 0 . 0001 ) , there was no correlation between IGFBP 3 and any of the markers of bone turnover ( OC , BALP , TRAP , or PICP ) nor with PTH or BMD at any site . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The increase in IGFBP 3 fragmentation correlated negatively with alterations in IGF 1 and IGF 2 ( P < 0 . 05 ) . ^^^ In addition , changes in IGFBP 3 protease activity because of cancer may contribute to alterations in IGF 1 and 2 , indicating the importance of measuring this parameter in addition to IGFs and IGFBPs when evaluating alterations in IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Anti TGF beta increased concentrations of IGF 1 5 . 8 fold and IGFBP 2 and IGFBP 3 by 8 and 7 fold in conditioned media whereas IGFBP 4 decreased 5 fold . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Food restriction abated serum IGF 1 concentrations , serum IGFBP 3 expression and hepatic IGF 1 mRNA levels at d 50 . ^^^ Conversely , at d 120 , hepatic IGF 1 mRNA levels in R were similar to C despite decreased serum IGF 1 concentrations and serum IGFBP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All subjects underwent a 24 h GH profile ( 20 minute sampling ) , measurement of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and growth hormone binding protein ( GHBP ) and , after an overnight fast , an AST ( intravenous arginine 20 g / m2 over 30 minutes ) . ^^^ Normative data for serum IGF 1 , IGF 2 , IGFBP 3 and IGFBP 2 were obtained from 125 subjects aged 60 87 years . ^^^ Serum IGF 1 , IGFBP 2 , and IGFBP 3 levels were significantly lower in the patients compared with the normal range , 102 ( 14 162 ) vs . 142 ( 59 298 ) micrograms / l ( P < 0 . 0001 ) , 415 ( 122 1868 ) vs . 640 ( 140 2585 ) micrograms / l ( P = 0 . 0007 ) and 2 . 29 ( 0 . 81 3 . 75 ) vs . 2 . 59 ( 1 . 00 3 . 52 ) mg / l ( P = 0 . 009 ) , respectively . ^^^ CONCLUSIONS : Of the indices studied the arginine stimulation test is more effective than GH markers , such as IGF 1 or IGFBP 3 , or measurement of spontaneous GH secretion for diagnosing GH deficiency in adults over the age of 60 years . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma ( free and total ) IGF 1 and IGFBP 3 levels were not related to leptin levels in men or women . ^^^ No relation existed between leptin and ( free or total ) IGF 1 and IGFBP 3 in men or women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum growth hormone ( GH ) binding protein , IGF 1 and IGFBP 3 in patients with beta thalassaemia major and the effect of GH treatment . ^^^ In this further study we have evaluated serum growth hormone ( GH ) binding protein ( GHBP ) , IGF 1 and IGFBP 3 in patients with beta thalassaemia major and the effects of GH treatment on these various parameters . ^^^ MEASUREMENTS : Serum GHBP , IGF 1 and IGFBP 3 were measured in all the patients . ^^^ RESULTS : The mean serum GHBP concentrations were normal in both prepubertal and pubertal children but the serum IGF 1 and IGFBP 3 concentrations were low throughout childhood and adolescence . ^^^ There was a significant correlation between serum IGF 1 and IGFBP 3 concentrations ( r = 0 . 79 ; P = 0 . 0001 ) but there was no correlation between the height SDS of the patients with serum GHBP , IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Immunosuppression induced a sustained significant increase in circulating IGFBP 3 and 30 kd IGFBPs in uninephrectomized and controls rats , whereas serum IGF 1 levels were unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Arm span , serum IGF 1 and IGFBP 3 levels as screening parameters for the diagnosis of growth hormone deficiency in patients with myelomeningocele preliminary data . ^^^ In a group of 38 prepubertal patients with MMC and hydrocephalus aged 3 . 8 11 . 0 years , auxological parameters , including arm span and bone age , and serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels were measured . ^^^ Serum IGF 1 and IGFBP 3 levels were normal ( > or = 10th percentile ) in 14 / 15 patients . ^^^ Serum IGF 1 and IGFBP 3 levels were normal in 10 / 12 of short statured patients with normal arm span , but low ( < 10th percentile ) in those patients with reduced arm span ( IGF 1 : 8 / 11 patients , P < 0 . 05 ; IGFBP 3 : 9 / 11 patients , P < 0 . 005 ) . ^^^ In 7 / 11 short statured MMC patients with reduced arm span and low serum IGF 1 and IGFBP 3 levels , growth hormone secretion was investigated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Estradiol increased serum IGF 1 , IGFBP 3 , and IGFBP 4 throughout the treatment period , but it did not influence other IGFBPs in serum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 levels for normal Thai children and their usefulness in clinical practice . ^^^ Serum IGF 1 and IGFBP 3 levels are growth hormone ( GH ) dependent and reflect the endogenous GH secretion . ^^^ Two hundred and forty four healthy children were evaluated for serum IGF 1 and IGFBP 3 levels and then the age defined normal values for Thai children were constructed . ^^^ The results showed that the serum IGF 1 and IGFBP 3 levels were age dependent , gradually increased from birth and reached the peak values around the age of 14 16 years . ^^^ In addition , we studied the IGF 1 and IGFBP 3 values in 28 GH deficient children and 26 normal variant short stature ( NVSS ) by using our normal constructed values as the reference . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGFBP 3 were measured by RIA . ^^^ Urinary GH levels as well as serum levels of IGF 1 and IGFBP 3 declined with age . ^^^ Urinary GH levels as well as serum levels of IGF 1 and IGFBP 3 correlated positively with BMC , even if age was taken into account . ^^^ In contrast , serum levels of IGF 1 and IGFBP 3 correlated positively with fat and % fat . ^^^ LBM did not correlate with either urinary GH or serum IGFBP 3 levels but exhibited a weakly positive correlation with serum IGF 1 level . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present results show that ovarian hormones and dietary protein content affect the plasma concentrations of IGF 1 , IGFBP 3 and IGFBP 4 and hepatic mRNA of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 4 in different manners . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report that the administration of hCG to female rats resulted in a significant increase in serum IGFBP 3 levels at doses greater than 10 UI and a significant decrease in serum IGF 1 levels . ^^^ Treatments greater than 10 UI of hCG resulted in a significant suppression of mammary IGFBP 3 and IGF 1 receptor expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Stimulation of DNA synthesis by IGF 1 is enhanced in bovine mammary epithelial cells that overexpress the IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At 13 . 5 days post coitus ( dpc ) the IGF system colocalizes with apoptosis areas ; IGFBP 2 , 4 and 5 are found in the interdigital zone , while IGFBP 3 and IGF 1 border this region . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This increase was associated with a decrease in insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) concentrations during treatment with octreotide , but not with a decrease in insulin concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and Glu Pg do not compete for IGFBP 3 binding ; instead , the binary IGFBP 3 / Glu Pg complex binds IGF 1 with high affinity ( Kd = 0 . 47 nM ) to form a ternary complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Infused IGF I / IGFBP 3 complex causes glomerular localization of IGF 1 in the rat kidney . ^^^ Rats were treated with intravenous injections of 125I labeled IGF 1 , 125I IGFBP 3 , or 125I IGFBP 4 alone or with complexes of 125I IGF 1 and IGFBP 3 or IGFBP 4 . ^^^ We conclude that the cellular localization of IGF 1 within the kidney can be directed to glomerular elements if the IGF 1 is given with IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : In a cross sectional population based study of 218 healthy elderly subjects ( age 55 80 yrs ) fasting free and total insulin like growth factor ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels were measured . ^^^ Free and total IGF 1 , IGFBP 1 and IGFBP 3 levels , were not different in subjects with the lowest and the highest Disability Index Score . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The HaCaT keratinocyte cell line , which has many characteristics of basal keratinocytes , synthesizes IGFBP 3 that in vitro reduces its IGF 1 responsiveness . ^^^ IGFBP 3 has attracted interest as a potential growth arrest protein , both via its ability to modulate IGF 1 responsiveness , and more controversially via IGF 1 independent mechanisms . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The relationship between peak growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , IGF 1 binding protein 3 ( IGFBP 3 ) and IGFBP 3 protease activity was studied in 28 children and adolescents undergoing investigation of pituitary function 0 . 4 14 . 2 years after cranial or craniospinal irradiation for the treatment of CNS tumours distant from the hypothalamic pituitary axis ( n = 16 ) or prophylaxis against CNS leukaemia ( n = 12 ) . ^^^ Low serum IGF 1 but normal IGFBP 3 concentrations and in the majority normal IGFBP 3 protease activity was found in patients in the years after CNS irradiation . ^^^ Neither serum IGF 1 nor IGFBP 3 can be used as a reliable index of the development of radiation induced GHD . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Monitoring of glucose , insulin , IGF 1 , IGF binding protein 3 , type 1 procollagen , osteocalcin , and glycosylated hemoglobin levels was performed in a subset of patients . ^^^ Plasma concentrations of IGF 1 , IGF binding protein 3 , procollagen , osteocalcin , and glycosylated hemoglobin increased with GH therapy , whereas glucose and insulin levels did not change . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH , IGF 1 , IGFBP 3 and IGFBP 2 in cerebrospinal fluid of infants , during puberty and in adults . ^^^ During diagnostic lumbar punctions cerbrospinal fluid ( CSF ) was collected for the determination of GH , IGF 1 , IGFBP 3 and IGFBP 2 . ^^^ GH was not correlated to IGF 1 , IGFBP 3 or IGFBP 2 . ^^^ However , IGF 1 was strongly related to IGFBP 3 ( r = 0 . 529 ; < 0 . 001 ) and IGFBP 2 ( r = 0 . 796 ; < 0 . 001 ) as was IGFBP 3 to IGFBP 2 ( r = 0 . 685 ; < 0 . 001 ) , suggesting dependence of the three variables . ^^^ With IGFBP 3 or IGFBP 2 as control variables ( partial correlation ) IGF 1 was no longer related to the binding proteins , while the relation of IGFBP 3 to IGFBP 2 remained unchanged with IGF 1 as the control variable ( r = 0 . 687 ; < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 concentrations correlated with body weight , thus reflecting breed size as previously shown , whereas IGF 2 concentrations did not . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes in serum IGF 1 and IGFBP 3 concentrations during the IGF 1 generation test performed prospectively in children with short stature . ^^^ Children with partial GH resistance would be expected to have short stature , elevated GH levels and relatively low levels of IGF 1 and IGFBP 3 . ^^^ The IGF 1 generation test in particular may demonstrate impaired secretion of IGF 1 and IGFBP 3 . ^^^ IGF 1 and IGFBP 3 were measured at 0900 h on day 0 and 4 of the IGF 1 generation test . ^^^ GH and IGF 1 were measured by radioimmunoassay , IGFBP 3 by IRMA and basal GHBP by HPLC . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : The evaluation included two standard GH provocative tests , GHRH test , night time GH secretion , GHBP ; and IGF 1 , IGFBP 3 and IGFBP 1 before and after 0 . 1 and 0 . 3 U / kg / day of rhGH given i . m . , for 4 days . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal serum insulin like growth factor 1 ( IGF 1 ) and IGFBP 3 ( binding protein 3 ) concentrations were also measured . ^^^ RESULTS : The patient group had a significantly lower peak GH response to both provocative tests ( P < 0 . 01 ) , and lower IGF 1 and IGFBP 3 levels compared with the normal controls ( P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The percentage of the total IGF 1 binding activity attributed to IGFBP 3 was greater ( p < 0 . 05 ) in myometrium , serum , and uterine fluid ( > 50 % ) than in inter ( 40 % ) and intracaruncular ( 37 % ) endometrium . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We attempted to analyse the relationships between bone mineral density ( BMD ) on the one hand and auxologic parameters , degree of siderosis , function of the growth hormone ( GH ) / insulin like growth factor 1 ( IGF 1 ) / IGF binding protein 3 ( IGFBP 3 ) axis , and calcium phosphate balance in 28 prepubertal children with SCD and 15 age matched children with constitutional short stature ( CSS ) . ^^^ Children with SCD had significantly decreased BMD ( 77 . 9 + / 11 . 9 per cent of normal BMD for age and sex ) and circulating concentrations of IGF 1 ( 91 + / 31 ng / ml ) and IGFBP 3 ( 1 . 7 + / 0 . 44 mg / l ) compared with the control group ( BMD = 93 . 5 + / 8 . 2 per cent of normal BMD for age and sex , IGF 1 = 221 + / 48 ng / ml , and IGFBP 3 = 2 . 3 + / 0 . 34 mg / ml ) . ^^^ BMD was correlated significantly with height , weight , and body mass index as well as with the circulating concentrations of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Glucose , insulin , leptin , free testosterone , delta 4 androstenedione , sex hormone binding globulin ( SHBG ) , LH , FSH , IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) were measured in the sera taken before the administration of glucose , while glucose and insulin levels were measured in all samples which were collected after the administration of glucose . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At birth IUGR newborns showed levels of GH and IGFBP 1 significantly higher , and IGF 1 , IGFBP 3 , and C peptide significantly lower than control subjects . ^^^ At 2 mo GH and IGFBP 1 levels decreased , whereas IGF 1 , IGFBP 3 , and C peptide rose , attaining the concentrations found in control subjects at birth . ^^^ These results suggest that : 1 ) the IGF related growth promoting mechanism is impaired in IUGR children at birth but is fully restored at 2 mo ; 2 ) the cord blood levels of GH , IGF 1 , IGFBP 3 , IGFBP 1 , and C peptide are not predictive of the weight and length gain during the first 3 mo of life ; 3 ) IUGR children have at least two different IGFBP 3 proteases , one cation dependent protease that is present at birth and able to yield the major approximately 29 kD IGFBP 3 fragment and a second one , with a different activation timing , which exhibits cation independence and induces the formation of a approximately 18 kD IGFBP 3 form . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the association of plasma insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) with risk of breast cancer in a study of 94 cases of premenopausal ductal carcinoma in situ and 76 controls . ^^^ After grouping women on the basis of both IGF 1 and IGFBP 3 , women in the highest two tertiles of IGF 1 and the lowest tertile of IGFBP 3 were at notably higher risk than women in the lowest tertile of IGF 1 and the highest two tertiles of IGFBP 3 ( odds ratio = 3 . 7 ; 95 % confidence interval = 1 . 1 12 . 2 ) . ^^^ We conclude that the combination of high IGF 1 and low IGFBP 3 may increase the risk of premenopausal ductal carcinoma in situ . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and leptin were significantly reduced compared with the reference values ( p < 0 . 001 , p < 0 . 01 and p < 0 . 001 , respectively ) . ^^^ In conclusion , the low spontaneous GH secretion rate and a disturbed GH secretion pattern , together with low serum levels of IGF 1 , IGFBP 3 and leptin , might contribute to the reduced postnatal growth in some of the subgroup of children born SGA who remained short during childhood . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Bioactivity of recombinant IGF 1 in transgenic mouse milk extracts was demonstrated by a concentration dependent increase in [ 3H ] thymidine incorporation in clonal bovine mammary epithelial cells ( MAC T ) compared with control mouse milk extracts ; moreover , addition of excess recombinant human insulin like growth factor binding protein 3 ( rhlGFBP 3 ) abolished the increase in [ 3H ] thymidine incorporation attributed to recombinant IGF 1 in transgenic mouse milk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured lower leg length and markers of bone formation [ bone alkaline phosphatase ( ALP ) and procollagen type 1 C terminal propeptide ( PICP ) ] , bone resorption [ pyridinoline , deoxypyridinoline , and carboxyl terminal telopeptide of type 1 collagen ( ICTP ) ] , soft tissue turnover [ procollagen type 3 N terminal propeptide ( P3NP ) ] , and the GH axis [ IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 2 , and urinary GH ] at 1 to 4 week intervals from diagnosis to week 27 of treatment . ^^^ At diagnosis , mean SD scores were : bone ALP , 1 . 84 ; PICP 1 . 77 ; pyridinoline , 1 . 42 ; deoxypyridinoline , 1 . 66 ; ICTP , 0 . 42 ; P3NP , +1 . 45 ; GH , +24 . 4 ; IGF 1 , 1 . 70 ; IGFBP 3 , 0 . 88 ; IGFBP 2 , +2 . 42 ; and GH binding protein , 0 . 69 . ^^^ During induction and intensification , there was shrinkage of the lower leg , with decreases in PICP , pyridinoline , ICTP , and P3NP ( P < 0 . 05 ) , whereas IGF 1 and IGFBP 3 increased ( P < 0 . 05 ) . ^^^ After prednisolone was discontinued , bone ALP and collagen markers increased markedly ( P < 0 . 01 ) , but there was no significant change in IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relationship between non enzymatic glycosylation and changes in serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in patients with type 2 diabetes mellitus . ^^^ The possible occurrence of increased non enzymatic glycosylation of serum insulin like growth factor binding protein 3 ( IGFBP 3 ) in vivo and the changes that would simultaneously occur in serum levels of IGFBP 3 and insulin like growth factor 1 ( IGF 1 ) were investigated . ^^^ We measured levels of IGF 1 and IGFBP 3 and the degree of glycation of total serum protein and IGFBP 3 , in serum samples obtained from patients with poorly controlled non insulin dependent diabetes ( type 2 ) and from age matched non diabetic controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGFBP profile of Mn animals reflected their elevated GH status , as we observed increased binding of tracer ( 125I IGF 1 ) to the circulating IGFBP 3 complex ( 120 % of control binding ) using native chromatography techniques . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have recently described a competitive binding assay for rat insulin like growth factor binding protein 3 ( IGFBP 3 ) based on the ability of IGFBP 3 to form a ternary complex with the acid labile subunit ( ALS ) in the presence of IGF 1 . ^^^ Nonfasting serum levels of IGFBP 3 were compared with those of total ( extractable ) IGF 1 ( tIGF 1 ) and ALS as well as IGFBP 3 determined by ligand blotting . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Traditionally , measurement of plasma IGF 1 and more recently of IGFBP 3 are used to distinguish GHD from idiopathic short stature in slowly growing children , using a single blood sample . ^^^ In earlier studies it was claimed that IGFBP 3 was superior to IGF 1 , but more recently doubts around this claim have arisen . ^^^ METHODS : Serum IGF 1 , IGF 2 , IGFBP 3 and various molar ratios were , after correction for age and sex using SD scores , compared to the maximum serum GH peak after two standard provocation tests using four different methods ( t test , chi 2 , likelihood ratios and ROC curves ) . ^^^ RESULTS : IGF 1 performed better than IGFBP 3 , but the best results were achieved by the molar ratio IGF 1 : IGF 2 . ^^^ However , IGFBP 3 correlated better with the short term response to GH therapy than IGF 1 or the ratios , and none of the parameters investigated was found to be related to the response of long term GH therapy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 1 was reduced ( 216 ng / mL ) , although still in the high range , whereas IGF 1 ( 71 ng / mL ) , IGFBP 3 ( 0 . 62 microg / mL ) , and IGF 2 ( 111 ng / mL ) were only slightly increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the circulating levels of GH , insulin like growth factor 1 ( IGF 1 ) , GH binding protein ( GHBP ) , and IGF binding protein 3 ( IGFBP 3 ) before L T 4 therapy in 19 infants with congenital hypothyroidism ( CH ) , aged 12 29 days , diagnosed by neonatal screening and in a group of age and sex matched control infants . ^^^ Serum GHBP was measured by the high performance liquid chromatography gel filtration method ; serum GH , IGF 1 , and IGFBP 3 levels were determined by commercial kits . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although the treatment also enhanced the developmental increase in IGF binding protein 3 ( IGFBP 3 ) , IGF 1 was increased proportionately more , resulting in a significantly higher molar ratio of IGF 1 : IGFBP 3 in treated females throughout the course of the study . ^^^ These data suggest that the premature elevation in IGF 1 levels , and consequently the ratio of IGF 1 : IGFBP 3 , accelerates the tempo of the final stages of puberty in rhesus monkeys . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein ( IGFBP 3 ) were below the 0 . 1 percentile . ^^^ Subsequently , the girl showed a marked catch up growth ; however , IGF 1 and IGFBP 3 remained below the 0 . 1 percentile . ^^^ The interpretation of low IGF 1 and IGFBP 3 levels in short children still requires good clinical judgement and basic knowledge of their biological action . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of our study was to evaluate concentration of IGF 1 and binding proteins : IGFBP 1 and IGFBP 3 in postmenopausal period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The SS was compared with other nutritional parameters , including sequential anthropometric indices , serum albumin , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and whole body protein turnover ( WBPT ) using [ 1 ( 13 ) C ] leucine . ^^^ There was no correlation with anthropometric indices or IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In all subjects , the free form of IGF 1 ( free IGF 1 ) , the total IGF 1 ( total IGF 1 : free plus complexed form of IGF 1 ) and IGFBP 3 in serum or plasma were measured . ^^^ The Z scores of free IGF 1 , total IGF 1 , and IGFBP 3 were calculated . ^^^ None of the Z scores for free IGF 1 , total IGF 1 or IGFBP 3 had a significant correlation with BS . ^^^ None of the Z scores for free IGF 1 , total IGF 1 or IGFBP 3 had a significant correlation with 1 , 5 AG , fructosamine or HbA 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Here , fIGF 1 and total IGF 1 ( tIGF 1 ) , the f / t IGF 1 ratio and proteolysis of IGFBP 3 were studied in the circulation of patients with precocious puberty and normal pubertal subjects , and they were compared with those of prepubertal and adult populations . ^^^ Compared to the f / t IGF 1 in the prepubertal and adult populations , no increase in f / t IGF 1 ratios was observed , and the proteolysis of IGFBP 3 was not detected in sera from the pubertal children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patients underwent standard growth hormone ( GH ) stimulation studies and measurement of IGF 1 and IGFBP 3 . ^^^ The mean + / SE for maximum stimulated GH value , IGF 1 and IGFBP 3 were 9 . 2 + / 1 . 2 ng / dl , 67 + / 6 ng / ml , and 1 . 7 + / 0 . 22 mg / L , respectively . ^^^ Also , the average values of IGF 1 and IGFBP 3 were significantly increased as compared to pretreatment values ( 186 + / 37 ng / ml and 3 . 0 + / 0 . 22 mg / L , p < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , circulating IGF 1 and IGFBP 3 levels were restored by IGF 1 infusion to levels similar to those in untreated CTRL rats . ^^^ In conclusion , restoration of normal circulating levels of IGF 1 and IGFBP 3 by rhIGF 1 infusion fails to reverse the growth retardation induced by zinc deficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Chronic LPS administration ( 250 microg / kg daily for 8 days ) significantly decreased body weight , serum levels of IGF 1 and pituitary GH content , whereas it increased circulating IGFBP 3 ( 47 kDa band ) , IGFBP 1 and IGFBP 2 ( 33 kDa band ) and the 24 kDa band ( which possibly corresponds to IGFBP 4 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating levels of IGF 1 , IGFBP 3 and ALS , and their degree of association in the ternary complex in the fetus increased with gestational age . ^^^ In serum from deliveries > 35 weeks both ALS and IGFs were limiting but approximately 25 % of IGFBP 3 was unable to form the ternary complex even in the presence of excess ALS and IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To address this question , we performed a cross sectional study measuring serum levels of IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , IGFBP 4 and IGFBP 5 by specific immunoassays in 52 adults with Type 1 ( n=27 ) and Type 2 ( n=25 ) diabetes mellitus and 100 age and sex matched healthy blood donors . ^^^ Type 1 diabetics showed significantly lower IGF 1 ( 119+ / 8 ng / ml ) and IGFBP 3 ( 2590+ / 104 ng / ml ) but higher IGFBP 1 levels ( 38+ / 10 ng / ml ) compared with Type 2 patients ( 170+ / 13 , 2910+ / 118 , 11+ / 3 respectively ; P < 0 . 05 ) or healthy controls ( 169+ / 5 , 4620+ / 192 , 3 . 5+ / 0 . 4 respectively ; P < 0 . 01 ) . ^^^ IGF 1 correlated positively with IGFBP 3 and IGFBP 5 and negatively with IGFBP 1 and IGFBP 4 in all subjects . ^^^ Furthermore , biochemical markers indicating bone loss ( ICTP ) and increased bone turnover ( PTH , OSC ) correlated positively with IGFBP 1 and IGFBP 4 but negatively with IGF 1 , IGFBP 3 and IGFBP 5 , while the opposite was observed with bone formation markers ( PICP , B ALP ) and vitamin D 3 metabolites . ^^^ In 20 Type 2 patients in whom immunoreactive proinsulin could be detected , significant positive correlations were found between proinsulin and BMD of hip ( r=0 . 63 , P < 0 . 005 ) , IGF 1 ( r=0 . 59 , P < 0 . 01 ) as well as IGFBP 3 ( r=0 . 49 , P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of our in vitro experiments with isolated porcine ovarian follicles was to study the effects of gonadotropins , GH , IGF 1 and oxytocin ( OT ) on release of ovarian steroid , OT , IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , prostaglandin F ( PGF ) , prostaglandin E ( PGE ) and cAMP . ^^^ GH ( 10 ng / ml or above ) was able to inhibit androstenedione , OT , PGF and IGFBP 3 , to stimulate IGF 1 and cAMP , and to alter testosterone and PGE release by follicles cultured in serum supplemented and / or serum free medium . ^^^ IGF 1 ( 10 ng / ml or more ) inhibited androstenedione and PGF secretion , stimulated testosterone , estradiol , OT and cAMP production , but did not influence progesterone , IGFBP 3 or PGE output in these conditions . ^^^ OT ( 100 ng / ml ) was able to inhibit androstenedione and to stimulate testosterone , IGF 1 , PGF and PGE , but not estradiol or IGFBP 3 release . ^^^ On the other hand , the different patterns of action of GH and IGF 1 on OT , estrogen and IGFBP 3 suggest that part of the GH effect on ovarian cells is IGF 1 independent . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Renal transplant recipients have significantly elevated serum of IGF 1 and IGFBP 3 compared to normal individuals . ^^^ When transplant recipients with and without posttransplant erythrocytosis were compared , similar levels of IGF 1 were found ; however , the group with erythrocytosis had significantly elevated IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
With the development of accurate insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) assays , additional tools are now available for assessing postoperative GH secretion . ^^^ There has , however , never been a systematic comparison of sensitive GH , IGF 1 , and IGFBP 3 assays in defining disease status in a large cohort of postoperative patients with acromegaly . ^^^ Sixty postoperative subjects with acromegaly and 25 age matched healthy subjects were evaluated with nadir GH levels after 100 g oral glucose as well as baseline IGF 1 and IGFBP 3 levels . ^^^ IGFBP 3 levels correlated overall with IGF 1 levels ( r = 0 . 765 ; P < 0 . 0001 ) , but IGFBP 3 levels were not predictive of disease status because 32 % of the subjects with active acromegaly had normal IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In adult female monkeys , serum concentrations of insulin like growth factor 1 ( IGF 1 ) are decreased by estradiol replacement , whereas levels of IGF binding protein 3 ( IGFBP 3 ) are increased . ^^^ Furthermore , chronic IGF 1 supplementation elevates serum IGFBP 3 despite a suppression of GH . ^^^ Serum IGF 1 and IGFBP 3 were significantly suppressed in GHa compared with Con females . ^^^ In Con females , estradiol replacement significantly decreased serum IGF 1 and increased serum IGFBP 3 . ^^^ In contrast , estradiol replacement significantly elevated both serum IGF 1 and IGFBP 3 in GHa females . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 were low ( 5 . 2 + / 2 . 0 ng / mL and 0 . 42 + / 0 . 13 microg / mL , respectively ; mean + / SD ) but rose normally with GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the majority of the plasma IGFBP 3 remaining after suramin was not the intact high affinity IGF binding form but rather a 30 kDa fragment with markedly reduced affinity for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the influence of 24 hour hypoxia ( 10 % oxygen ) , 48 hour hyperoxia ( 75 % oxygen ) , and relative hypoxia ( shifting from 48 hours in 75 % oxygen to 24 hours in room air ) on the gene expression of IGF 1 , IGF 1 receptor ( IGF IR ) , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 in retina using a solution hybridization RNase protection assay . ^^^ Hypoxia induced a significant increase in retinal IGF IR ( 178 % ) , IGFBP 2 ( 227 % ) , and IGFBP 3 ( 317 % ) mRNA ; however , retinal IGF 1 mRNA was reduced , as well as serum growth hormone ( GH ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have therefore investigated the effects of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) and intact IGFBP 3 on in vitro sperm motility . ^^^ Washed motile sperm selected by the ' swim up ' method , from normozoospermic samples , were incubated at 37 degrees C in 5 % CO 2 in air with IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , or control Earle ' s media , and were examined at time 0 and after 60 min incubation . ^^^ RESULTS : Compared to controls , statistically significant changes occurred at time 60 min after incubation : IGF 1 decreased CV and ALH significantly ( P < 0 . 05 ) , but IGFBP 3 increased Ln , St , BF , and decreased ALH significantly ( P < 0 . 05 ) . ^^^ CONCLUSIONS : IGF 1 and IGFBP 3 have differing and opposing effects on in vitro sperm motility parameters and thus may have a role in modulating in vivo sperm motility . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of peripubertal children after renal transplantation ( RTX ) with recombinant human growth hormone : auxological data and effects on insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) during 24 months . ^^^ Serum IGF 1 was determined by RIA , IGFBP 3 by RIA and Western ligand blotting ( WLB ) . ^^^ The ratio IGF I / IGFBP 3 rose during the first year ( p = 0 . 002 ) indicating more bioavailable IGF 1 . ^^^ This may be due to significant changes in IGF 1 and IGFBP 3 relationship . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fetal plasma IGFBP 3 and IGF 1 concentrations were significantly ( P < 0 . 05 ) reduced at day 115 of gestation after maternal undernutrition . ^^^ The degree of reduction in fetal plasma IGFBP 3 and IGF 1 between day 105 and 115 of gestation as a response to acute maternal undernutrition was significantly greater ( P < 0 . 05 ) in fetuses of mothers receiving low periconceptual nutrition . ^^^ The response of maternal plasma IGFBP 3 and IGF 1 to undernutrition did not depend on the level of periconceptual nutrition . ^^^ These results suggest that exposure to maternal periconceptual undernutrition reprograms IGFBP 3 and IGF 1 regulation in the developing sheep fetus , altering its response to undernutrition in late gestation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Low insulin , IGF 1 and IGFBP 3 levels in children with Prader Labhart Willi syndrome . ^^^ It is well established that insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) and insulin are low in growth hormone deficiency , but due to their dependence on nutrition , they are elevated in healthy obese children . ^^^ As the presence of growth hormone deficiency in Prader Labhart Willi syndrome ( PWS ) is still controversial , we studied insulin , IGF 1 and IGFBP 3 levels in 19 children with PWS ( age range 0 . 5 14 . 6 years ) . ^^^ Serum concentrations of insulin ( SDS : 0 . 7+ / 0 . 9 , P = 0 . 01 ) and IGF 1 ( SDS : 0 . 7+ / 0 . 8 , P = 0 . 002 ) were low , but IGFBP 3 ( SDS : 0 . 3+ / 1 . 2 , P = 0 . 2 ) was normal compared to normal weight age matched children . ^^^ Since children with PWS are typically obese , insulin , IGF 1 and IGFBP 3 levels should be compared to normal obese children who present increased levels of these hormones . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Therefore , we examined in 25 healthy athletes the effects of three different durations of three types of exercise incremental ergometer cycling exercise ( ICE ) , long distance Nordic ski race ( NSR ) and a treadmill simulated soccer game ( TSG ) lasting 20 min , 3 h , and 2 10 45 min separated by a 15 min half time rest respectively , on plasma concentrations of growth hormone ( [ GH ] ) , insulin like growth factor 1 ( [ IGF 1 ] ) and its binding proteins 1 and 3 ( [ IGFBP 1 ] , [ IGFBP 3 ] ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 inhibited and IGFBP 5 augmented IGF 1 induced proliferation . ^^^ We conclude that the expression and time dependent production of IGFBP 3 , IGFBP 4 , and IGFBP 5 and their regulation by endogenous TGF beta 1 represent mechanisms by which human intestinal muscle cells regulate autocrine IGF 1 mediated growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) serum concentrations provide a good measure of the biological effects of growth hormone . ^^^ The aims of the present study were to : ( 1 ) investigate the associations of IGF 1 and IGFBP 3 with body fat mass and distribution , and ( 2 ) evaluate the effects of 3 weeks of very low calorie diet ( VLCD ) ( 318 kcal / day , with 40 g protein , 35 g carbohydrate , and 2 g fat ) on IGF 1 and IGFBP 3 serum concentrations . ^^^ RESULTS : Before dietary treatment , IGF 1 and IGFBP 3 serum concentrations were inversely associated with visceral adipose tissue ( VAT ) area ( p < 0 . 005 and p < 0 . 05 , respectively ) , but not with either total body fat or subcutaneous adipose tissue area . ^^^ VLCD produced a significant decrease of body mass index ( p < 0 . 001 ) , total body fat ( p < 0 . 001 ) , VAT ( p < 0 . 005 ) , subcutaneous adipose tissue ( p < 0 . 001 ) , IGF 1 concentrations ( p < 0 . 05 ) , and an increase of IGFBP 3 serum levels ( p < 0 . 001 ) . ^^^ The association of VAT with either IGF 1 or IGFBP 3 serum concentrations was not maintained following VLCD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Serum levels of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , E 2 , and progesterone . ^^^ RESULT ( S ) : Levels of IGF 1 , IGFBP 3 , E 2 , and progesterone did not differ between women with prospectively documented PMS and control subjects . ^^^ Levels of IGF 1 and IGFBP 3 did not differ between women with PMS and control subjects , supporting the concept that PMS and endogenous depression are biologically distinct entities . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Serum GH , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , insulin , glucose , total testosterone , sex hormone binding globulin , E 2 , and gonadotropin levels . ^^^ Moreover , patients with ovarian hirsutism have decreased IGFBP 3 concentrations , which might enhance IGF 1 bioavailability . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dose related effects of growth hormone on IGF 1 and IGF binding protein 3 levels in non islet cell tumour hypoglycaemia . ^^^ We report the effect of increasing doses of rhGH on the clinical condition and serum IGF 1 and IGFBP 3 levels in two patients with solitary pleural fibrous tumours causing severe hypoglycaemia . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , a bone promoting peptide , may be considered as reflecting osteoblast function as well as its main binding protein , insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ In this study , we aimed to measure the circulating IGF 1 and IGFBP 3 in AS patients . ^^^ We determined the serum levels of growth hormone ( GH ) , insulin , glycaemia , and the IGF 1 and IGFBP 3 serum concentrations . ^^^ CONCLUSIONS : Since IGFBP 3 is an important cofactor for IGF 1 and modulates its bioavailability and activity in bone , these data suggest that osteoblast cell function could be impaired in AS . ^^^ Inflammation could play a role in this IGFBP 3 / IGF 1 axis involvement . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor 1 ( IGF 1 ) is predominantly bound in the trimeric complex comprised of IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) . ^^^ Circulating concentrations of IGF 1 , IGFBP 3 and ALS are believed to reflect the GH secretory status , but the clinical use of ALS determination is not known . ^^^ We found : 1 ) no significant arteriovenous gradient over the liver ofALS , IGF 1 , and IGFBP 3 ; 2 ) the diurnal variation of ALS was 12 % ( mean coefficient of variation percent ) ; 3 ) ALS levels increased throughout childhood with maximal levels in puberty , with a subsequent decrease with age in adults ; and 4 ) ALS levels were below 2 SD in 57 of 79 GHD patients ( sensitivity 72 % ) and above 2 SD in 22 of 29 patients with normal GH response ( specificity 76 % ) , which was similar , compared with the diagnostic utility of IGF 1 and IGFBP 3 . ^^^ Finally , our findings indicate that hepatic ALS production is not measurable by this approach or , alternatively , that the liver is not the primary source of circulating ALS , IGF 1 , or IGFBP 3 in humans . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Male rats exhibited increases in serum IGF 1 and IGFBP 3 concentrations before increases in serum testosterone levels . ^^^ IGF 1 and testosterone peaked at 9 weeks of age , and thereafter remained in a steady state , whereas IGFBP 3 reached a peak at 7 weeks of age , and then gradually declined . ^^^ A strong correlation between serum IGF 1 and IGFBP 3 levels was found in subjects 3 9 weeks old . ^^^ In females , serum 17 beta estradiol , IGF 1 and IGFBP 3 levels increased gradually from 3 to 5 weeks old , peaked at 9 weeks , and then decreased slowly thereafter . ^^^ The correlation coefficient between serum IGF 1 and IGFBP 3 was highly significant . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the GH bioactivity ( GH BIO ) , GH serum level by immunofluorimetric assay ( GH IFMA ) , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 in 21 prepubertal obese children ( 13 boys and eight girls ) aged 5 . 7 to 9 . 4 years affected by simple obesity and in 32 ( 22 boys and 10 girls ) age and sex matched normal weight controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : We determined the effects of continuous combined nonoral and oral estrogen progestin therapy on plasma levels of insulin like growth factor 1 ( IGF 1 ) and its binding proteins 1 ( IGFBP 1 ) and 3 ( IGFBP 3 ) . ^^^ Transdermal estrogen in combination with intrauterine levonorgestrel did not induce any change in plasma IGF 1 , whereas the plasma levels of IGFBP 3 decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum DHEA , DS , sex steroids , IGF 1 , IGFBP 1 , IGFBP 3 , growth hormone binding protein ( GHBP ) levels and lipid profiles as well as body composition ( by DEXA ) and muscle strength ( by MedX testing ) were measured at baseline and after each treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH increases serum concentrations of IGF 1 and IGFBP 3 . ^^^ The combined effects of GH and anabolic androgenic steroids on IGFBP 3 and IGF 1 levels are not known under authentic substance abusing conditions . ^^^ MEASUREMENTS : Serum IGF 1 concentration was measured by radioimmunoassay and the IGFBP 3 concentration was measured by two immunofluorometric assays , one detecting proteolytic fragments of IGFBP 3 . ^^^ RESULTS : While growth hormone increased the IGFBP 3 and IGF 1 concentrations , anabolic steroids decreased the same . ^^^ Concomitant use of growth hormone and anabolic steroids decreased the IGFBP 3 concentration in five out of six abuse periods in spite of the fact that the IGF 1 concentration remained elevated in four of them . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : In four females known with congenital partial lipodystrophy , hyperinsulinaemia with acromegaloid features , the number and affinity of the IGF 1 receptors on peripheral blood mononuclear cells ( PBMCs ) , and the concentration of circulating insulin , total and free IGF 1 , IGFBP 1 and IGFBP 3 levels were measured in the fasting and the fed state . ^^^ Total IGF 1 , free IGF 1 and IGFBP 3 levels did not differ but serum IGFBP 1 levels were lower in lipodystrophy subjects than in healthy controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Responsiveness of IGF 1 and IGFBP 3 to therapeutic intervention in children and adolescents with Crohn ' s disease . ^^^ We have studied the concentrations of the inflammatory marker CRP and of serum IGF 1 and IGFBP 3 in patients with active Crohn ' s disease and have assessed the changes in these parameters during therapeutic intervention with enteral nutrition or intestinal resection . ^^^ Serum concentrations of CRP , IGF 1 and IGFBP 3 were determined at 0 , 2 , 8 and 16 weeks after start of enteral nutrition and in addition to height velocity , at 0 and 6 months after intestinal resection . ^^^ MEASUREMENTS : Study A : weight SDS , CRP , IGF 1 and IGFBP 3 were measured at 0 , 2 , 8 , 16 weeks after start of enteral feeding . ^^^ Study B : height velocity , CRP , IGF 1 and IGFBP 3 were measured 0 , 6 months after intestinal resection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Cryostat sections of colon from TNB treated and control rats were hybridized with 35S labeled antisense probes for IGF 1 , IGFBP 3 , IGFBP 4 and IGFBP 5 . ^^^ Altered expression of IGFBP 3 , IGFBP 4 and IGFBP 5 in specific bowel layers during colitis suggests that they play a role in modulating IGF 1 action . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 significantly inhibit gonadotropin induced ovulation and oocyte maturation by neutralizing endogenously produced IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Biosynthesis of IGFBP 3 was stimulated dose dependently by IGF 1 and 2 , while IGFBP 1 , 2 and 4 were not affected . ^^^ The increase of IGFBP 3 in response to IGF 1 and 2 was due to a stimulation of IGFBP 3 specific mRNA as well as to an inhibition of IGFBP 3 endocytosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To this purpose , the effects of the LHRH agonist Zoladex ( LHRH A ) on 1 ) the mitogenic action of IGF 1 , 2 ) the tyrosine phosphorylation of type 1 IGF 1 receptor ( IGF IR ) , 3 ) the concentration of IGF IR , and 4 ) the secretion of IGF binding protein 3 were studied . ^^^ The results obtained show that in DU 145 cells , LHRH A 1 ) counteracts the mitogenic action of IGF 1 in a dose dependent manner , 2 ) prevents the IGF 1 induced tyrosine phosphorylation of the IGF IR , 3 ) reduces the concentration of IGF IR without affecting its Kd value , and 4 ) does not affect the secretion of IGF binding protein 3 in the conditioned medium from these cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Stimulation was half maximal at 1 . 1 nM and completely inhibited by an IGF 1 antagonist and by IGF binding protein 3 ( IGFBP 3 ) . ^^^ Possible paracrine regulation was confirmed by stimulation of acinar cell proliferation with fibroblast conditioned medium , which was partially inhibited by IGF 1 antagonist or by IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The contribution of these peptides to the acquisition and maintenance of bone mineral is less certain since volumetric bone density was preserved despite low levels of IGF 1 and IGFBP 3 associated with GH resistance . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 mRNA levels increase , while IGFBP 3 and 5 mRNA and protein levels dramatically decrease . ^^^ CONCLUSION : Our results suggest that following spinal cord injury , overexpression of IGF 1 and underexpression of IGFBP 3 and 5 may lead to hyperplasia of the smooth muscle layer of the bladder . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Other results were : IGF 1 , 3 11 ng / mL ( normal , 114 492 ) ; IGF 2 , 185 299 ng / mL ( normal , 358 854 ) ; IGF binding protein 1 ( IGFBP 1 ) , 12 200 ng / mL ( normal , 13 73 ) ; IGFBP 2 , 60 384 ng / mL ( normal , 55 480 ) ; and IGFBP 3 , 400 600 ng / mL ( normal , 2000 4000 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum free insulin like growth factor 1 ( IGF 1 ) , total IGF 1 , and IGF binding protein 3 concentrations in normal children and children with growth hormone deficiency . ^^^ The GH induced increase in the serum free IGF 1 levels was significantly higher than those of the total IGF 1 and IGF binding protein 3 levels . 6 ) The percent increase in the serum free IGF 1 level after 1 month of GH treatment showed a significant positive correlation with that of the GH induced improvement in the percent increase in the height velocity during 1 yr of GH therapy ( r = 0 . 526 ; P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 5 , and total protein were determined in extracts obtained after ethylenediamine tetraacetate and guanidine hydrochloride extraction . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recombinant human IGFBP 3 induced an apoptotic effect that was similar to the PKCalpha antisense oligonucleotide , and its effect was blocked by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to assess the GH IGFI axis , GH receptor availability , as reflected by the levels of GH BP , and the amount of GH dependent IGFBP 3 in adult IDDM patients with different degrees of metabolic control . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since insulin like growth factor 1 ( IGF 1 ) mediates the effect of growth hormone and has been positively associated with prostate cancer , we have evaluated the role of this hormone and its principal binding protein , IGFBP 3 , in the aetiology of childhood leukaemia . ^^^ Blood measurements of IGF 1 and IGFBP 3 were undertaken using commercially available radioimmunoassays . ^^^ There was no significant association between IGF 1 and the likelihood of childhood leukaemia , but an increment of 1 microg / ml of IGFBP 3 was associated with a substantial and statistically significant reduction of childhood leukaemia by 28 % ( 95 % confidence interval 7 % to 45 % ) . ^^^ Because IGFBP 3 is essentially a binding protein , we interpret our findings as indicating that bioavailable IGF 1 may play an important role in the aetiology of childhood leukaemia . ^^^ The much smaller quantities and the inherent instability of IGF 1 in the blood in comparison to those of IGFBP 3 are likely to hinder documentation of an underlying positive association of IGF 1 with the disease . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Data were also analyzed using multiple logistic regression with adjustment for known risk factors for the development of breast cancer as well as anthropometric , demographic and hormonal variables , including estradiol , prolactin , IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the effects of menopause and three different formulations of E 2 plus medroxyprogesterone acetate on serum concentrations of basal and growth hormone releasing hormone ( GHRH ) stimulated growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP ) 1 , IGFBP 3 , insulin , and C peptide . ^^^ MAIN OUTCOME MEASURE ( S ) : Levels of GH , IGF 1 , IGFBP 1 , IGFBP 3 , insulin , and C peptide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This concept was used to investigate whether 1 ) GH influences the FSH threshold for monofollicular growth and 2 ) whether such an influence would depend upon the endogenous GH / insulin like growth factor 1 ( IGF 1 ) / IGF binding protein 3 ( IGFBP 3 ) levels . ^^^ IGF 1 levels as well as IGFBP 3 levels significantly increased ( P < 0 . 01 ) during GH substitution . ^^^ The FSH area under the curve was negatively correlated to IGF 1 ( r = 0 . 6 ; P < 0 . 05 ) and IGFBP 3 ( r = 0 . 6 ; P < 0 . 05 ) . ^^^ The results of this study indicate that GH may play a role in the physiological growth of the follicle ; most likely this occurs by influencing the IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Filter paper blood spot assay of human insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 and preliminary application in the evaluation of growth hormone status . ^^^ To facilitate broader applications of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) analysis , we developed procedures for their measurements in extracts of whole blood dried on filter paper . ^^^ Blood spot assays showed intra and interassay coefficients of variation ( including interspot variations ) of 5 . 4 16 . 7 % for IGF 1 and 6 . 6 11 . 7 % for IGFBP 3 ; recoveries were 97 + / 7 . 1 % and 101 + / 8 . 7 % , respectively . ^^^ Recoveries of IGF 1 and IGFBP 3 in response to 4 to 8 fold variations in extraction buffer volume were 97 + / 8 . 2 % and 107 + / 6 . 1 % , respectively . ^^^ Dried blood spot IGF 1 and IGFBP 3 showed greater than 1 month stability at 20 C , 4 C , and room temperature and retained more than 65 % of the immunoreactivity after approximately 1 month at 37 C . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Over 75 % of the circulating insulin like growth factors ( IGF 1 and 2 ) are bound in 140 kDa ternary complexes with IGF binding protein 3 ( IGFBP 3 ) and the 84 86 kDa acid labile subunit ( ALS ) , a glycoprotein containing 20 kDa of carbohydrate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The subcutaneous administration of rhIGF 1 at the single dose of 0 . 4 mg / kg revealed a half life of IGF 1 as short as 90 minutes , and her serum IGFBP 3 level was extremely low . ^^^ The results suggested that the treatment with a high dose of rhIGF 1 by both SI and CSI is effective for preventing the postnatal growth retardation and normalizing glucose metabolism in patients with genetic form of extremely severe insulin resistant diabetes and that IGF 1 deficient state and partial GH resistance such as the impairment of the production of IGF 1 and IGFBP 3 may contribute to the growth retardation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of insulin like growth factor ( IGF ) 1 and Des ( 1 3 ) IGF 1 on the level of IGF binding protein 3 and IGF binding protein 3 mRNA in cultured porcine embryonic muscle cells . ^^^ Treatment of porcine myogenic cultures with 20 ng of IGF 1 or 20 ng of Des ( 1 3 ) IGF I / ml serum free media for 24 h results in a threefold reduction in the level of IGFBP 3 in conditioned media . ^^^ This IGF 1 induced decrease in IGFBP 3 mRNA level appears to be relatively unique to myogenic cells . ^^^ The simultaneous decrease in IGFBP 3 mRNA and protein that accompanies IGF 1 induced myogenin expression suggests that differentiation of myogenic cells may be preceded or accompanied by decreased production of IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma insulin like growth factor 1 ( IGF 1 ) levels were higher in girls treated with CBZ and OXC than in the control girls , but AEDs did not affect fasting serum insulin , IGF binding protein 1 , or IGF binding protein 3 concentrations in girls on VPA , CBZ , or OXC medication during the period of exposure ( average 2 . 8 , 4 . 1 , and 1 . 9 years , respectively ) in this study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The antiproliferative IGFBP 3 activity is not IGF independent , since des ( 1 3 ) IGF 1 , which does not bind to IGFBP 3 , reverses its inhibitory effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similarly , CF secretion of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 were depressed , whereas CF collagen synthesis ( [ 3H ] proline incorporation ) and PTC secretion of the fibrogenic cytokines , transforming growth factor beta 1 , and platelet derived growth factor were unaffected . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PURPOSE : Insulin like growth factor ( IGF 1 ) and its major binding protein ( IGF BP 3 ) have recently been implicated in the pathogenesis of several malignancies . ^^^ SUBJECTS AND METHODS : This cross sectional study examines the relationship of a series of epidemiologic parameters ( age , sex , height , body mass index , smoking , alcohol consumption , and coffee drinking ) with IGF 1 and IGF BP 3 in a sample of 130 healthy adults . ^^^ RESULTS : We observed that serum levels of IGF 1 are higher , whereas levels of IGF BP 3 are lower , in men than in women . ^^^ CONCLUSION : Age , sex , and smoking are independent predictors of IGF 1 and / or IGF BP 3 . ^^^ The influence of these epidemiologic variables on the pathogenesis of disease states associated with IGF 1 and IGF BP 3 warrants further exploration . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ternary complex formation by the glycovariants in the presence of ALS and excess IGF 1 was not significantly different from that of fully glycosylated recombinant human ( rh ) IGFBP 3 [ Ka ( fully glycosylated ) =12 . 5+ / 4 . 1 l / nmol ; mean Ka ( all mutants ) =22 . 1+ / 3 . 0 l / nmol ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Granulosa cells from small ( 1 to 5 mm ) follicles were collected from cattle , cultured for 2 d in medium containing 10 % fetal calf serum , washed , and then treated for an additional 2 d in serum free medium with follicle stimulating hormone ( FSH ) ( 50 ng / ml ) , recombinant human IGF 1 ( 0 , 1 . 3 , 4 . 0 , or 13 . 3 nM ) , or recombinant human IGFBP 3 ( 0 to 4 . 26 nM ) . ^^^ In one series of experiments , IGFBP 3 ( 0 . 53 and 2 . 13 nM ) inhibited ( 51 % to 92 % decreases ; P < 0 . 05 ) progesterone and estradiol production induced by 1 . 3 nM of IGF 1 , but did not influence ( P > 0 . 10 ) granulosa cell numbers or steroidogenesis in the absence of IGF 1 . ^^^ Only 4 . 26 nM of IGFBP 3 inhibited ( by 35 % ) the increase in granulosa cell numbers induced by 1 . 3 nM of IGF 1 . ^^^ In another series of experiments , 13 . 3 nM of IGF 1 , but not 4 . 0 nM of IGF 1 , was able to completely overcome the inhibitory effect of 4 . 26 nM of IGFBP 3 on estradiol production . ^^^ The increase in cell numbers induced by 4 . 0 and 13 . 3 nM of IGF 1 was attenuated ( P < 0 . 001 ) by 4 . 26 nM of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Chronic liver disease was characterised by low serum IGF 1 , IGF 2 , IGFBP 3 and ALS levels with raised IGFBP 1 and 2 levels . ^^^ After OLT , there was a rapid normalisation of serum IGF 1 , while IGF 2 and IGFBP 3 overshot to supranormal levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Monthly measurements of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in healthy prepubertal children : characterization and relationship with growth : the 1 year growth study . ^^^ The usefulness of measurements of IGF 1 or IGF binding protein 3 ( IGFBP 3 ) in the clinical management of growth disorders is dependent on the extent of physiologic variation in their concentrations . ^^^ Our purpose was therefore to investigate the longitudinal intraindividual variation in serum concentration of IGF 1 and IGFBP 3 in healthy prepubertal children . ^^^ Concentrations of IGF 1 and IGFBP 3 were measured by RIA . ^^^ These monthly longitudinal variations in IGF 1 and IGFBP 3 were parallel to changes in longitudinal growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to evaluate longitudinally time dependent changes in the GH response to GHRH , IGF 1 , IGFBP 3 and albumin values in patients during corticotherapy . ^^^ IGF 1 , IGFBP 3 and albumin were determined in each test , at time 0 . ^^^ There were no differences in basal GH values , GH response to GHRH , IGF 1 and IGFBP 3 levels between controls and patients before starting corticotherapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Evaluation of disease activity by IGF 1 and IGF binding protein 3 ( IGFBP 3 ) in acromegaly patients distributed according to a clinical score . ^^^ To facilitate the estimation of acromegalic activity a prospective study was done comparing , against a clinical score , the effectiveness of serum IGF 1 , IGFBP 3 and the IGF I / IGFBP3 molar ratio . ^^^ Increases in serum IGF 1 and IGFBP 3 levels were similarly frequent : both 100 % in group 1 , 80 % and 95 % in group 2 , 9 % and 36 % in group 3 , respectively . ^^^ Log IGF 1 , log IGFBP 3 , and log molar ratio correlated significantly with the clinical scores ( r=0 . 873 , r=0 . 692 , and r=0 . 829 , respectively ) . ^^^ Conclusions : The IGF I / IGFBP3 molar ratio was not better than either IGF 1 or IGFBP 3 in detecting activity in the three groups of patients studied . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A 6 mo trial on the s . c . infusion regimen significantly reduced growth hormone secretion ( as judged by IGF 1 and IGFBP 3 concentrations ) , and normalized growth velocity overcoming the pubertal growth spurt . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition ( assessed by bioelectrical impendance analysis [ BIA ] ) , plasma GHBP ( fast protein liquid chromatography [ FPLC ] size exclusion gel chromatography ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( [ IGFBP 3 ] radioimmunoassays ) were measured before and after 6 months . ^^^ In contrast to placebo therapy , GH replacement therapy increased the mean plasma levels of IGF 1 and IGFBP 3 to the normal range , whereas a small but statistically significant increase in plasma GHBP was observed . ^^^ The combination of baseline plasma GHBP , body fat mass , and IGFBP 3 predicts posttreatment GHBP levels accurately ( adjusted R 2 = . 97 ) , indicating that baseline variables such as age , gender , fat free mass , and IGF 1 have no contribution . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : GH status was evaluated by 12h spontaneous GH release during the night time , arginine stimulated GH release and the basal concentrations of IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ RESULTS : No significant differences were found in spontaneous and stimulated GH release in CF patients compared with healthy controls , whereas IGF 1 and IGFBP 3 were significantly decreased in CF patients compared with healthy controls . ^^^ The combination of reduced IGF 1 and IGFBP 3 with normal GH release points to a relative GH resistance or a disturbance in the pituitary axis in patients with CF . ^^^ The spontaneous GH release , the stimulated GH release and the basal concentrations of IGF 1 and IGFBP 3 were not significantly different in diabetic CF patients compared with CF patients with normal glucose tolerance and the presence of diabetes mellitus was not consistent with increased GH resistance in CF patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline GH secretion and plasma levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were at the lower limit of normal , but increased with GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGFBP 3 , 4 and 5 and acid labile subunit ( ALS ) were therefore examined in 20 children with growth hormone ( GH ) insensitivity before and after 6 months of therapy with recombinant human IGF 1 ( 80 or 120 micrograms / kg twice daily ) . ^^^ These findings indicate that GH is responsible for the regulation of serum levels of IGFBP 3 , 4 and 5 and ALS , and that IGF 1 does not directly regulate the concentrations of these circulating IGFBPs . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although patients with GH insensitivity and elevated GH binding protein ( GHBP ) levels have been described , it may be a reasonable approach to screen children who have growth failure , low levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 , and low levels of GHBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Short term increments of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 predict the growth response to growth hormone ( GH ) therapy in GH sensitive children . ^^^ Basal and generated serum levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , measured in an IGF generation test following four or seven daily injections of growth hormone ( GH ) , 0 . 1 IU / kg ( 0 . 033 mg / kg ) , were analysed in these patients . ^^^ Strong correlations were observed between basal and generated IGF 1 and IGFBP 3 levels . ^^^ The most valuable parameters were the increases in IGF 1 and IGFBP 3 levels in the generation test , which excluded 95 . 2 % of the patients from a diagnosis of GHIS . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A cohort of 18 patients was evaluated for the impact of treatment on serum IGF 1 , free IGF 1 , IGF binding protein 3 levels , and total IGF binding capacity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In liver and gastrocnemius muscle , mRNAs for IGF 1 , IGFBP 2 , and IGFBP 3 were analyzed in young and aged males of two strains , BN / F344 and Sprague Dawley . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth hormone binding activity was measured , as well as the following biochemical markers of the somatotropic axis : GH suppression induced by oral administration of glucose , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ Although octreotide treatment induced a decrease in the levels of GH , IGF 1 , and IGFBP 3 , as well as an increase in the level of GHBP , these biochemical markers did not reach normal levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As for the peripheral limb of the GH insulin like growth factor 1 ( IGF 1 ) axis , high free IGF 1 , low IGF binding proteins 1 ( IGFBP 1 ) and 2 ( IGFBP 2 ) , normal or high IGFBP 3 and increased GH binding protein ( GHBP ) circulating levels have been described in obesity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of total and free IGF 1 and IGFBP 3 are increased and maintained in long term training . ^^^ The ratio of total IGF 1 to IGFBP 3 was not increased by training in any group . ^^^ These data indicate that serum levels of total and free IGF 1 and total IGFBP 3 can be increased with intense training and maintained with reduced training ( tapering ) . ^^^ The findings show that changes in free IGF 1 levels are not accounted for by alterations in the total IGF I / IGFBP 3 complex or in IGFBP 3 levels and indicate that there are other important determinants of free IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 levels for the assessment of disease activity of acromegaly . ^^^ To assess the disease activity of acromegaly in patients , we measured the changes in serum growth hormone ( GH ) levels during oral glucose tolerance test and the basal serum levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in 29 acromegalic patients and 30 health persons served as normal controls . ^^^ Serum levels of IGF 1 and IGFBP 3 significantly correlated with each other in the normal subjects as well as the patients . ^^^ Both IGF 1 and IGFBP 3 levels were significantly higher in the group of patients with active acromegaly than inactive acromegalic patients and the normal subjects but there was not statistically difference between the normal controls and the inactive acromegalics . ^^^ While serum IGF 1 levels presented considerable overlapping instances among the three groups , the serum IGFBP 3 of inactive patients and the normal controls could rarely reach 4 . 44 ng / ml , the lowest value of the active acromegalics . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : We compared IGF 1 and IGFBP 3 concentrations in a group of adults treated for brain tumours in childhood with those of matched controls , and investigated the relationship between the GH secretory pattern and IGF 1 / IGFBP3 concentrations in the entire group . ^^^ DESIGN : We performed 24 h serum GH profiles using 20 minute sampling and measured corresponding fasting concentrations of IGF 1 and IGFBP 3 . ^^^ MEASUREMENTS : IGF 1 and IGFBP 3 concentrations were measured at 06 . 00 hours . ^^^ RESULTS : Serum IGF 1 levels were significantly lower in the irradiated group ( Irradiated : 200 micrograms / l vs Control : 265 micrograms / l ; P = 0 . 001 ) but the difference in serum IGFBP 3 ( Irradiated : 2 . 3 mg / l vs Controls : 2 . 77 mg / l ; P = 0 . 03 ) was less marked . ^^^ Peak GH activity was lower in the Irradiated subjects ( 2 . 59 mU / l vs 9 . 04 mU / l ; P = 0 . 0004 ) and correlated strongly with IGF 1 ( r = 0 . 62 ; P = 0 . 002 ) but less so with IGFBP 3 ( r = 0 . 35 ; P = 0 . 09 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with IGF 1 or its analogue des ( 1 3 ) IGF 1 did not lead to any consistent change in IGFBP 3 abundance . ^^^ However , both IGF 1 and des ( 1 3 ) IGF 1 at 100 ng / ml led to a modest increase in IGFBP 3 mRNA levels in keratinocytes , suggesting posttranscriptional regulation of IGFBP 3 abundance . ^^^ We propose that local modulation of IGFBP 3 abundance may represent another level of regulation of growth factor action in the epidermis , where EGF and TGFbeta 1 and possibly other local growth factors specifically regulate the availability of IGF 1 to its keratinocyte receptors . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Leptin , insulin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured by specific immunoassays . ^^^ There were also significant increments in insulin , IGF 1 , and IGFBP 3 , whereas IGF 2 , IGFBP 1 , and IGFBP 2 did not change over 9 months of GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were determined at the time of retesting and 6 , 12 , and 24 months after discontinuation of treatment in the patients with permanent GHD and after 6 months in those with normal GH responses to stimulation . ^^^ Mean IGF 1 and IGFBP 3 concentrations at the time of retesting were significantly higher in groups 1 and 2 than in groups 3 and 4 . ^^^ In patients of groups 3 and 4 , mean IGF 1 was significantly decreased after 6 and 12 months , whereas IGFBP 3 was significantly decreased 12 months after treatment withdrawal . ^^^ IGF 1 and IGFBP 3 determinations shortly after GH withdrawal had limited value in the diagnosis of GHD of childhood onset associated with congenital hypothalamic pituitary abnormalities , but became accurate after 6 12 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Low serum levels of free and total insulin like growth factor 1 ( IGF 1 ) in patients with anorexia nervosa are not associated with increased IGF binding protein 3 proteolysis . ^^^ IGF 1 action is modulated by IGF binding proteins ( IGFBPs ) , and a variety of catabolic states has been characterized by the presence of increased IGFBP 3 proteolysis . ^^^ In AN , the levels of GH binding protein , free and total IGF 1 , free IGF 2 , and IGFBP 3 were significantly reduced ; total IGF 2 , IGFBP 2 , and IGFBP 4 levels were unchanged ; and IGFBP 1 was increased . ^^^ In conclusion , the mechanisms responsible for the adaption of the GH IGF IGFBP axis in AN may be different from other catabolic conditions , because the low levels of free and total IGF 1 in AN are not associated with increased IGFBP 3 proteolysis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To study this phenomenon we have investigated the endocrine status , including IGF 1 and its binding proteins IGFBP 1 and IGFBP 3 , in children referred for follow up at various time intervals after surgery . ^^^ IGF 1 , IGFBP 3 , IGFBP 1 and prolactin serum concentrations were evaluated in fasting samples . ^^^ Fasting serum IGF 1 and IGFBP 3 levels were normal in 8 and 12 of the 31 measurements , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , the increase in serum IGF 1 , IGF 2 , and triiodothyronine correlated with the increase in IGFBP 3 . ^^^ CONCLUSION : GH significantly increased serum IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 4 and decreased serum IGFBP 1 and IGFBP 2 in post menopausal women . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In fact , GH , IGF 1 and IGFBP 3 levels in older people are clearly reduced and similar to those observed in patients with GH deficiency . ^^^ MEASUREMENTS : Group 1 : blood samples for IGF 1 and IGFBP 3 assay were drawn basally and 12 h after rhGH administration ( 20 micrograms / kg ) . ^^^ Group 2 : blood samples for IGF 1 , IGFBP 3 , glucose and insulin assays were drawn basally , 12 h after the first and the last rhGH administration ( 5 micrograms / kg ) . ^^^ RESULTS : Basal IGF 1 levels were lower in ES ( whole group ) than in YS ( whole group ) ( 123 . 1 + / 8 . 9 vs . 230 . 4 + / 16 . 1 micrograms / l , P < 0 . 001 ) while IGFBP 3 levels in the two groups were similar ( 2 . 7 + / 0 . 2 vs . 3 . 1 + / 0 . 2 mg / l ) . ^^^ No sex related differences in IGF 1 and IGFBP 3 levels were recorded in either group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factors IGF 1 and IGFBP 3 in children with slipped capital femoral epiphysis . ^^^ Concentrations of IGF 1 and its binding protein 3 ( IGFBP 3 ) were measured in the serum of 19 SCFE children without endocrine disorders . ^^^ Concentrations for IGF 1 and IGFBP 3 were predominantly within the normal ranges for chronologic age and bone age . ^^^ The correlation of IGF 1 and IGFBP 3 serum levels was high . ^^^ Serum concentrations of IGF 1 and IGFBP 3 provided no evidence of a disturbance of the somatotropic axis in SCFE children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , IGF 1 or IGF 2 ( 6 . 5 nM ) preferentially induced the accumulation of IGFBP 3 ( 1 . 9 fold ) and to a lesser extent of IGFBP 4 , but did not show any effect on IGFBP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This proteolysis was less pronounced for IGFBP 3 containing a mutated heparin binding domain , and was prevented by purifying IGFBP 3 on an IGF 1 affinity column in the presence of 2 M sodium chloride , suggesting that the responsible protease ( s ) binds the IGFBP 3 heparin binding domain . ^^^ To determine if any human proteases act this way , we first studied plasma prekallikrein since it can copurify with IGFBP 3 , and found : 1 ) [ 125 ] IGFBP 3 binds to prekallikrein immobilized either on nitrocellulose or on immunocapture plates ; 2 ) the IGFBP 3 heparin binding domain participates in forming the IGFBP 3 / prekallikrein complex ; 3 ) the binary IGFBP 3 / prekallikrein complex can bind IGF 1 to form a ternary complex ; and 4 ) activation of prekallikrein to alpha kallikrein by Factor XIIa resulted in proteolysis of bound IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To measure the serum concentrations of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , and the level of IGFBP 3 protease activity in 38 children presenting with malignancies , and to assess their relation with auxological parameters and nutritional status . ^^^ IGF 1 and IGFBP 3 were measured using specific radioimmunoassays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High blood levels of an abnormal form of insulin like growth factor type 2 ( IGF 2 ) associated with low blood levels of insulin , growth hormone , IGF 1 , and IGF BP 3 were observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Mean concentrations of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were low , but most values were within age specific reference values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our objective was to investigate the effect of recombinant human insulin like growth factor binding protein 3 ( rhIGFBP 3 ) or insulin like growth factor 1 ( IGF 1 ) antibodies on the IGF 1 related mitogenic activity of bovine serum and of mammary tissue extracts in primary mammary epithelial cell cultures . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No differences were seen between the two diabetic groups with respect to body weight , food intake , blood glucose , serum GH , IGF 1 , and IGFBP 3 levels or hepatic IGF 1 levels . ^^^ In conclusion , administration of a GHR antagonist in diabetic mice has renal effects without affecting metabolic control and circulating levels of GH , IGF 1 , or IGFBP 3 , thus indicating that the effect of G120K PEG may be mediated through a direct inhibitory effect on renal IGF 1 through the renal GHR . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Secreted , recombinant IGFBP 3 ( IGFBP 3 ( Ad ) ) was purified from the culture medium of virus infected cells by IGF 1 affinity chromatography followed by reverse phase HPLC . ^^^ In addition , IGFBP 3 ( Ad ) was detected by immunoblot using an antibody specific for human IGFBP 3 and by ligand blot using radiolabeled IGF 1 . ^^^ IGFBP 3 ( Ad ) had similar affinities for IGF 1 and ALS and an approximately 25 % decreased affinity for IGF 2 compared to IGFBP 3 ( Pl ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Anabolic hormone status was assessed by the ratio of testosterone ( T ) to sex hormone binding globulin ( SHBG ) , given as the free androgen index ( FAI ) , and the ratio of insulin like growth factor 1 ( IGF 1 ) to IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Steady state levels of IGFBP 3 mRNA were quantified by RNase protection assay and were compared with the level of IGF 1 class 1 and class 2 transcripts . ^^^ The short term nature of this treatment suggests that the increase in hepatic IGFBP 3 and IGF 1 transcripts is a relatively early response to treatment with GH , and that the increase in plasma concentrations of IGFBP 3 in response to GH are derived from the liver , the kidney , or both . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moderate doses of porcine somatotropin do not increase plasma insulin like growth factor 1 ( IGF 1 ) or IGF binding protein 3 . ^^^ IGFBP 3 was greater ( 1 . 09 vs . 0 . 78 micrograms / ml , P < 0 . 001 ) , whereas IGF 1 tended to be greater ( 206 vs . 176 ng / ml , P = 0 . 14 ) , in pigs from the small litters . ^^^ There was no effect of pST on plasma IGF 1 ( 182 vs . 195 ng / ml , P = 0 . 454 ) or IGFBP 3 ( 0 . 93 vs . 0 . 94 microgram / ml , P = 0 . 85 ) concentrations . ^^^ Plasma IGF 1 and IGFBP 3 were highly correlated with the growth rate of nursing pigs ( R = 0 . 638 and 0 . 756 , respectively ) . ^^^ In conclusion , pST had no little or no effect on growth performance or plasma IGF 1 , IGF 2 , or IGFBP 3 in sucking pigs on either a high or low plane of nutrition . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total alanine scanning mutagenesis of insulin like growth factor 1 ( IGF 1 ) identifies differential binding epitopes for IGFBP 1 and IGFBP 3 . ^^^ We have systematically investigated the binding contribution of each IGF 1 amino acid side chain toward IGFBP 1 and IGFBP 3 , combining alanine scanning mutagenesis and monovalent phage display . ^^^ Surprisingly , most IGF 1 residues could be substituted by alanines , resulting in less than 5 fold affinity losses for IGFBP 3 . ^^^ Instead , our results suggest that the N terminal backbone region of IGF 1 is important for binding to IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A possible role of IGFBP 3 proteolysis in the ovine ULF may be to selectively increase the bioavailability of IGF 1 in the uterine microenvironment , which may be crucial for the rapid elongation of trophoblast that begins during days 12 15 after mating . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The purpose of this study was to determine if there were characteristic patterns of GH , IGF 1 , or IGFBP 3 levels in pediatric SBS patients with profound growth failure in an effort to elucidate an early strategic approach to management of SBS in the subpopulation . ^^^ METHODS : Forty patients ( 29 boys , 11 girls ; mean age , 5 . 3 years ; range , 0 . 5 to 18 . 6 years ) with SBS ( < 30 % total bowel length ) who received intensive nutrition support and follow up underwent serological tests for GH , IGF 1 , IGFBP 3 , and thyroid function . ^^^ Thirty eight percent of patients ( n = 11 ) had growth failure by anthropometry that was associated significantly with low IGF 1 independently and with both IGF 1 and IGFBP 3 levels ( P < 0 . 05 ) . ^^^ Low IGF 1 and IGFBP 3 but not GH levels may be indices of intestinal failure in pediatric SBS . ^^^ CONCLUSION : Exogenous IGF 1 and IGFBP 3 , not GH , may be beneficial to treat this subpopulation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To examine whether insulinlike growth factor 1 ( IGF 1 ) , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) in serum and peritoneal fluid ( PF ) correlate with the presence and severity of endometriosis . ^^^ Simultaneous sampling of blood and PF was performed during laparoscopy , and IGF 1 , IGF 2 and IGFBP 3 levels were determined by immunoradiometric assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dose response study of GH effects on circulating IGF 1 and IGFBP 3 levels in healthy young men and women . ^^^ In conclusion , our results demonstrate that IGF 1 and IGFBP 3 responses to rhGH are dose and sex dependent . ^^^ However , IGFBP 3 is less sensitive than IGF 1 to rhGH stimulation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , a polypeptide known to affect bone metabolism , and two of its binding proteins ( IGFBP 1 , IGFBP 3 ) were measured preoperatively and after 6 months . ^^^ We found consistently lower levels of IGF 1 and IGFBP 3 and a tendency to higher levels of IGFBP 1 in the patients with hip fractures , who also had a lower total body mass , lower fat mass and bone mineral density than the coxarthrosis group , indicating a more catabolic state in the patients with hip fracture , even 6 months after the trauma . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To investigate whether the severity of GHD was correlated with the degree of bone mass and turnover impairment , we evaluated BMD at the lumbar spine and femoral neck ; circulating insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and osteocalcin levels , and urinary cross linked N telopeptides of type 1 collagen ( Ntx ) levels in 101 adult hypopituitary patients and 35 sex and age matched healthy subjects . ^^^ In patients in group 2 , circulating IGF 1 and IGFBP 3 levels were not different from those in group 1 , whereas the t scores at the lumbar spine ( 1 . 22 + / 0 . 3 ) and femoral neck ( 0 . 9 + / 0 . 3 ) were significantly higher and lower , respectively , than those in groups 1 and 5 ( P < 0 . 05 ) but not those in groups 3 and 4 , and serum osteocalcin and urinary Ntx levels were significant higher than those in group 1 and lower than those in groups 3 5 ( P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared to control values , GH ( P < 0 . 0001 ) , IGF 1 ( P < 0 . 0001 ) , and IGFBP 3 ( P < 0 . 001 ) levels were increased , whereas testosterone ( P < 0 . 0001 ) and dihydrotestosterone levels ( P < 0 . 0001 ) were reduced in active acromegalic patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , we measured serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in the patients . ^^^ The serum IGF 1 and IGFBP 3 levels were low ( 152 + / 14 microg / L and 2 . 56 + / 0 . 12 mg / L , respectively ) , and the levels positively correlated with BMI ( r = 0 . 46 , P < 0 . 01 ; and r = 0 . 39 , P < 0 . 05 , respectively ) . ^^^ The serum IGFBP 2 , IGF 1 , and IGFBP 3 levels returned toward normal ranges as BMI in the patients improved during treatment . ^^^ The present study suggested that ALS was regulated by nutritional status , the same as IGF 1 , IGFBP 2 and IGFBP 3 ; but the serum IGFBP 2 was the best predictor of BMI as nutritional status marker among the parameters in patients with anorexia nervosa . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting levels of IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , sex hormone binding globulin , and free testosterone ( T ) were also obtained . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Biosynthetic hGH ( 0 . 1 IU / kg BW ) was administered s . c . for 4 days and blood samples were taken at baseline and the morning after the last GH injection for measurement of IGF 1 and IGFBP 3 . ^^^ IGF 1 ( 26 . 7+ / 4 . 6 nmol / l , mean+ / SD ) and IGFBP 3 ( 2 . 1+ / 0 . 2 mg / l ) levels were lower in the patients compared with the controls ( 43 . 0+ / 3 . 7 nmol / l and 2 . 8+ / 0 . 2 mg / l , respectively , P < 0 . 01 ) . ^^^ Following stimulation with exogenous hGH , there was a significant increase in IGF 1 and IGFBP 3 levels in the control group ( 85 and 73 % , respectively ) , but only a small increase in the patients ( 31 and 14 % ) . ^^^ It appears that stimulated and spontaneous GH secretion is normal in children with active systemic JCA , but the response to endogenous and exogenous GH with regard to IGF 1 and IGFBP 3 production is impaired , indicating a degree of GH insensitivity in such children . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , serum levels of GH , IGF 1 , IGFBP 3 and GHBP were measured before and under treatment and adverse events were assessed in treatment group . ^^^ Hormonal findings in the treated group showed a significant increase in IGF 1 and IGFBP 3 values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Competitive binding studies indicated that IGFBP 2 fragments had reduced binding affinity for IGF 1 . bFGF induced IGFBP 3 mRNA and protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The GH response to exercise was not correlated with fitness , mean GH or IGF 1 but was correlated with IGFBP 3 ( r = 0 . 65 , p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor ( IGF ) 1 , free IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 3 and insulin in obese children . ^^^ We investigated serum IGF 1 , free IGF 1 , IGFBP 1 , IGFBP 3 and insulin levels in 36 obese and 39 non obese healthy children . ^^^ IGFBP 3 levels correlated positively with IGF 1 ( r = 0 . 44 , p < 0 . 005 ) , and free IGF 1 levels ( r = 0 . 37 , p = 0 . 05 ) in the obese children . ^^^ We concluded that normal growth in obese children might be maintained through normal IGF 1 and increased IGFBP 3 levels , which are stimulated by increased insulin levels or nutritional factors or by increased responsiveness to GH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Based on the fact that no antibodies to IGF 1 were detected and on findings in previous studies , it is speculated that the increasing serum IGF 1 levels during long term IGF 1 treatment are caused by an increase in serum IGFBP 3 induced by chronic IGF 1 administration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Weight , height , overnight polysomnography , and IGF 1 and IGF binding protein 3 levels were evaluated before and 3 to 12 months after T & A . ^^^ Serum IGF 1 levels increased from 146 . 3 + / 76 . 2 ng / mL before T & A to 210 . 3 + / 112 . 5 ng / mL after surgery ( P < . 01 ) , but IGF binding protein 3 levels did not change significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , sex hormone binding globulin ( SHBG ) , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and testosterone were measured . ^^^ Also , there were significant ( P < 0 . 05 ) increments in serum testosterone , IGF 1 and IGFBP 3 levels from stages T 1 to T 4 . ^^^ PSA showed a positive correlation with testosterone ( r = 0 . 86 , P < 0 . 001 ) , IGF 1 ( r = 0 . 66 , P < 0 . 001 ) , and IGFBP 3 ( r = 0 . 34 , P = 0 . 004 ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : Cross sectional analysis of the relationship between insulin resistance syndrome variables and concentrations of IGF 1 , IGF 2 , IGFBP 1 and IGFBP 3 in 80 subjects with NIDDM . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Early morning serum levels of LH , FSH , oestradiol or testosterone , IGF 1 and IGF BP 3 were determined as well as several specific markers of bone turnover . ^^^ Mean IGF 1 and IGF BP 3 SDS scores were 1 . 24 and 0 . 78 respectively , significantly reduced . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To compare the effects of monthly intra muscular injections of a long acting preparation of octreotide , Sandostatin LAR , with multiple daily subcutaneous injections of octreotide and to study the interrelationships between mean 24 h growth hormone profile , serum total and free IGF 1 levels , 24 h urinary growth hormone levels and serum IGFBP 3 . ^^^ Serum total and free IGF 1 , serum IGFBP 3 and 24 h urinary GH were also measured on each occasion . ^^^ The relationships between mean 24 h GH levels and total IGF 1 and IGFBP 3 although significant showed less powerful correlations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Almost 90 per cent of haemangiopericytomas expressed IGF 1 receptor ( IGF IR ) mRNA ( 17 out of 19 ) , five ( 26 . 3 per cent ) expressed IGF binding protein 1 ( IGF BP 1 ) , three ( 15 . 8 per cent ) expressed IGF BP 2 , and four ( 21 per cent ) exhibited IGF BP 3 mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Estrogen administration suppressed peripheral markers of the IGF system , as evidenced by a negative correlation between plasma concentration of estradiol and IGF 1 ( r = 0 . 93 , P < 0 . 03 ) , and a trend for a similar relationship between plasma levels of estradiol and IGFBP 3 ( r = 0 . 86 , P = 0 . 06 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentrations measured by RIA on d 3 were 10 . 7+ / 0 . 4 ng / mL for IGF 2 , 1 . 9+ / 0 . 1 ng / mL for IGF 1 , 100+ / 5 ng / mL for IGF binding protein 3 ( IGFBP 3 ) , and 2163+ / 108 ng / mL for IGFBP 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 , dehydroepiandrosterone sulfate , testosterone , estradiol , and free androgen index all decreased significantly with age ( 54 , 17 , 76 , 26 , 33 , and 57 % , respectively ) , while sex hormone binding globulin and parathyroid hormone increased significantly with age ( 62 % and 43 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study used a cross sectional design to investigate relationships among serum insulin like growth factor ( IGF ) parameters ( total serum IGF 1 , IGF 2 , and IGF binding protein 3 ) , serum estradiol , and bone mineral density ( BMD ) stratified for potential confounders , and a longitudinal design to investigate the effects of hormonal replacement therapy ( HRT ) on IGFs and BMD . ^^^ In the follow up study , HRT decreased IGF 1 and IGF 2 , but did not influence the age related decline in IGF binding protein 3 significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum GH levels were elevated , and serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and GH binding protein were extremely reduced . ^^^ Administration of GH for 4 consecutive days resulted in a slight increment in serum IGF 1 levels , but no elevation of serum IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
During insulin withdrawal , IGFBP 1 increased by more than 6 fold ( from 32+ / 8 to 205+ / 17 ng / mL , P < 0 . 001 ) , IGFBP 3 increased significantly ( from 2631+ / 118 to 3053+ / 101 ng / mL , P < 0 . 001 ) , and total IGF 1 levels declined modestly ( from 226+ / 33 to 182+ / 26 ng / mL , P < 0 . 001 ) . ^^^ Within 2 h of insulin replacement , the changes in circulating concentrations of IGFBP 1 and IGFBP 3 were partially reversed , and free IGF 1 levels rebounded to 0 . 54+ / 0 . 22 ng / mL ( P < 0 . 1 vs . insulin withdrawal ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study we investigated the effects of a new route of administration , the intranasal E 2 spray ( S 21400 ) , on bone turnover and circulating IGF 1 and IGF binding protein 3 ( IGFBP 3 ) . ^^^ Serum IGF 1 and IGFBP 3 were measured at baseline , 1 month , and 3 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum steroids and gonadotropins were determined at 0 and 24 h and at 0 , 3 and 24 h after GnRH agonist challenge respectively , whereas IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 and sex hormone binding globulin were measured at baseline . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The authors studied the serum concentrations of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in 260 healthy children and adolescents ( 115 males , 145 females ) aged 5 20 years . ^^^ Serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in healthy Thai children and adolescents : relation to age , sex , and stage of puberty . ^^^ The serum IGF 1 and IGFBP 3 concentrations increased with age and peaked at age 13 15 years in males , and 11 13 years in females . ^^^ After the peak concentration , IGF 1 and IGFBP 3 levels declined significantly in males , but were still high in females . ^^^ Comparing between sexes , the concentrations of IGF 1 and IGFBP 3 were greater in females than males in all age groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Over this period , growth , serum total proteins , IGF 1 levels and IGFBP 3 / IGFBP 2 ratio were significantly decreased compared to the group fed ad libitum 18 % casein diet . ^^^ Nutritional rehabilitation with 18 or 50 % casein diet for one day did not initiate the restoration of serum IGF 1 levels and IGFBP 3 / IGFBP 2 ratio . ^^^ However , after 10 days with 18 or 50 % casein diets , there was an increase of 12 fold in IGF 1 levels and 7 fold in the IGFBP 3 / IGFBP 2 ratio . ^^^ Finally , rehabilitation for 21 days with 18 or 50 % casein diet produced an increase of 21 and 26 fold in IGF 1 levels , and 6 . 1 and 14 . 5 fold in the IGFBP 3 / IGFBP 2 ratio respectively . ^^^ Serum IGF 1 and IGFBP 3 concentrations were sensitive indicators of the evolution of the nutritional status of the rats depending of the protein concentration in the diet in previously undernourished rats . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^ IGF 1 , IGFBP 3 andALS showed a similar pattern of change associated with age . ^^^ Overall , IGF 1 , IGFBP 3 and ALS were slightly higher in females than in males . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To determine the overall plasma levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in a group of men at higher risk of prostate cancer development and to investigate the relationships between demographics and these levels , particularly with regard to race . ^^^ METHODS : An enzyme linked immunosorbant assay was used to quantitate plasma levels of IGF 1 and IGFBP 3 . ^^^ No correlation between IGFBP 3 plasma level and age was detected in the group as a whole , but an inverse relationship between IGF 1 plasma level and age was evident ( P = 0 . 0079 ) . ^^^ Since IGFBP 3 can control IGF 1 bioavailability , the lowered IGFBP 3 could explain in part the increased risk of prostate cancer in AA men . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We detected IGF 1 in the mucus covering the OE and Western ligand blot analysis ( WLB ) showed IGFBPs with an apparent Mr of 41 , 500 / 38 , 500 , 34 , 000 and 24 , 000 , which were immunoprecipitated by specific antisera to IGFBP 3 , 2 and 4 , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 levels were low ( 0 . 55 and 0 . 40 mg / I ) and did not increase after IGF 1 treatment , suggesting that this GH effect is not mediated by IGF 1 , and injected IGF 1 had a rapid disappearance rate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Leptin , glucose , insulin , free IGF 1 , total IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 levels were measured in the basal state after an overnight fast , and the acute insulin response to glucose ( AIRG ) was determined after intravenous glucose injection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Anthropometric data were collected : thyroid and pituitary glands were examined ; serum levels of thyroid stimulating hormone ( TSH ) , free thyroxin ( FT 4 ) , insulin like growth factor 1 ( IGF 1 ) and its binding protein 3 ( IGFBP 3 ) were measured . ^^^ Levels of IGF 1 and IGFBP 3 did not differ between the dose groups , but the high dose group had a lower IGF 1 / IGFBP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , PTH treatment resulted in an increase in the mRNA for aggrecan , a reduction in IGFBP 3 mRNA , and no discernible changes in IGF 1 mRNA levels , which was complemented by quantitative changes in IGFBP 3 and free IGF 1 levels . ^^^ Collectively , our results indicate that the effect of PTH may be mediated at least in part through the regulation of the IGF / IGFBP axis , by a decrease in the level of IGFBP 3 , and an increase in free IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total IGF 1 level in serum is a sensitive index during growth hormone ( GH ) replacement therapy of adults , since GH stimulates the hepatic expressions of both insulin like growth factor ( IGF 1 ) and acid labile subunit ( ALS ) and the major part of IGF 1 in the circulation is found in a ternary complex together with ALS and IGFBP 3 . ^^^ It is not clarified , whether the IGF 1 levels within normal range for age is due to endogenous basal GH production being sufficient or other factors stimulating IGF 1 , IGFBP 3 or ALS expressions . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition , IGF 1 , IGFBP 3 and ALS levels were measured before and after 3 months of rhGH therapy . ^^^ Body composition changed as expected most impressively in twin 1 ; twins 1 and 2 had low IGF 1 , IGFBP 3 and ALS prior to treatment with GH , but normal concentrations that were similar to those of their healthy twin were attained after 3 months of GH treatment . ^^^ Twin 3 had normal IGF 1 , IGFBP 3 and ALS concentrations before GH treatment , which were similar to those of her twin . ^^^ Insulin and GH have both been shown to stimulate hepatic production of IGF 1 and these results suggest that insulin may also be involved in the regulation of IGFBP 3 and ALS in vivo . ^^^ We conclude that clinical state , body composition and IGF 1 assessed together remain the best methods of monitoring GH replacement therapy and that measurements of IGFBP 3 and ALS do not confer any advantage over IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Notably , GH therapy raises both IGF 1 and IGFBP 3 levels in serum . ^^^ Until further research in the area dictates otherwise , ongoing surveillance and routine monitoring of IGF 1 and IGFBP 3 levels in GH recipients must become standard of care . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The insulin like growth factors IGF 1 and IGF 2 and their major binding protein IGFBP 3 influence the growth of breast cancer cells in vitro . ^^^ METHODS : Serum IGF 1 , IGF 2 and IGFBP 3 were measured by specific radioassay in 12 women with benign breast disease , 31 patients with breast cancer and in age matched controls . ^^^ However , the index of free unbound IGF measured as the molar ratio of IGF 1 plus IGF 2 divided by IGFBP 3 was significantly lower in benign breast disease than in the other subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied GH secretory patterns in the elderly by constructing 24 h serum GH profiles in 45 male and 38 female ( age , 59 . 4 73 . 0 yr ) volunteers and related patterns to IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and GH binding protein levels ; body mass index ; and waist / hip ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurements of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) are important in assessing the GH IGF axis . ^^^ As nearly all IGF 1 , IGFBP 3 , and ALS circulate in a GH dependent ternary protein complex , direct determination of the complex may be of significant analytical and clinical importance . ^^^ In pairwise mixed antibody evaluation , mAbs in G 2 and G 3 showed simultaneous binding to serum IGFBP 3 complexes in combination with an anti IGF 1 or an anti ALS antibody . ^^^ On this basis , two novel enzyme linked immunosorbent assays ( ELISAs ) involving IGFBP 3 / IGF 1 ( ELISA 1 ) and IGFBP 3 / ALS ( ELISA 2 ) recognition partners were developed , both demonstrating acceptable analytical performance characteristics . ^^^ IGFBP 3 complexes measured by ELISA 1 and 2 in samples from normal individuals and subjects with GH deficiency , acromegaly , and GH receptor deficiency more tightly correlated with IGF 1 , IGFBP 3 , and ALS than IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that MAbs against GH amplify and prolong the serum IGF 1 response to GH , which may result from both a prolongation of liver IGF 1 synthesis and an enhanced induction of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Twenty one children ( 10 girls , 11 boys ) with standard risk pre B ALL treated with chemotherapy had serum insulin like growth factor 1 ( IGF 1 ) , serum IGF binding protein 3 ( IGFBP 3 ) levels , and linear growth and weight data measured every 3 months during MT . ^^^ RESULTS : At the beginning of MT , the median IGF 1 standard deviation scores ( SDS ) and IGFBP 3 SDS were 0 . 52 and 0 . 09 , respectively , which declined during MT to 1 . 67 ( P < 0 . 001 ) and 1 . 82 ( P < 0 . 001 ) , respectively . ^^^ CONCLUSIONS : A significant decline in IGF 1 , IGFBP 3 , and growth retardation may not be directly related to the treatment intensity during MT . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have conducted a case control study on the island of Crete , Greece , to examine the potential relation between circulating IGF 1 and 2 and their major binding protein ( IGF BP 3 ) , on the one hand , and colorectal cancer , on the other . ^^^ IGF 1 , IGF 2 and IGF BP 3 were determined in the serum from 41 patients with colorectal cancer and 50 healthy controls ; data were analyzed using unconditional multiple logistic regression , adjusting for age , gender , education , height and BMI , as well as mutually . ^^^ Both IGF 1 and IGF 2 were positively , while IGF BP 3 was inversely , associated with risk for colorectal cancer , though none of these relations reached statistical significance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Plasma GH , IGF 1 and IGF binding protein 3 ( IGF BP 3 ) levels were measured sequentially in patients undergoing hepatectomy with or without cirrhosis . ^^^ Cirrhosis patients undergoing hepatectomy had low IGF 1 and IGF BP 3 levels , despite extremely high GH levels in the perioperative period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 binding protein 3 ( IGFBP 3 ) concentrations were significantly higher in 9 to 10 month old rats compared with that in 22 to 23 month old animals ( P < . 001 ) . ^^^ From these results , it was concluded that 1 ) NaSi 1 mRNA and protein levels are lower in old animals and increase in both adult and aged rats after hGH treatment , 2 ) plasma IGF 1 levels are similar in adult and aged rats and increase after hGH treatment , and 3 ) plasma IGFBP 3 levels are lower in old rats and remain unchanged after hGH treatment . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have , therefore , determined the concentrations of plasma IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in boars , barrows , and gilts and related these to postnatal growth characteristics . ^^^ Between 15 and 23 wk . of age , IGF 1 and IGFBP 3 , but not IGF 2 , were positively associated with growth rate , voluntary feed intake , and gain : feed ratio . ^^^ Plasma IGF 2 , but not IGF 1 or IGFBP 3 , was positively associated with backfat depth during this period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Oxytocin treated food restricted females had more body fat and lower plasma levels of IGF 1 , IGFBP 1 and IGFBP 3 compared with saline treated counterparts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Basal IGF 1 levels in DCM were lower ( P = 0 . 000039 ) than in CS ( 135 . 2 + / 46 . 8 vs . 193 . 7 + / 63 . 7 mu / l ) , whereas , basal IGFBP 3 and GHBP 2 levels in DCM and CS were similar ( 2 . 5 + / 1 . 3 vs . 2 . 6 + / 0 . 5 mg / l and 25 . 3 + / 3 . 6 vs . 28 . 3 + / 5 . 0 % ; P = 0 . 95 and P = 0 . 085 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Insulin like growth factor ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) are GH dependent and their concentrations have been used in the diagnosis of GH deficiency . ^^^ MEASUREMENTS : Serum levels of IGF 1 , free IGF 1 , IGFBP 3 and IGFBP 1 were measured by immunoradiometric assay . ^^^ During rhGH treatment the increase in serum total and free IGF 1 and IGFBP 3 paralleled the beneficial effects on body composition . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND PATIENTS : Body composition , plasma leptin , fasting and post oral glucose tolerance test insulin , IGF 1 and IGFBP 3 were studied in 34 MyD patients and 33 controls . ^^^ MEASUREMENTS : Body composition was measured using a bioelectrical impedance analyzer , and circulating levels of insulin , leptin , IGF 1 , IGFBP 3 were measured by IRMA or RIA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : IGFBP 1 and insulin were measured in samples drawn every 4 h over the 24 h period while IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in samples collected at the end of the study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Body composition measures , SHBG , testosterone ( T ) , free testosterone ( FT ) , dihydrotestosterone ( DHT ) , alpha 4 androstendione ( A ) , dehydroepiandrosterone sulphate ( DHEAS ) , 17beta oestradiol ( E 2 ) , oestrone ( E 1 ) , oestrone sulphate ( ES ) , 24 h integrated GH concentration ( ICGH ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP 3 ) were determined at baseline and after six months in women with Turner syndrome , and at baseline in control women . ^^^ Employing multiple regression analysis IGFBP 3 , ICGH , DHEAS and fat free mass explained 85 % ( adjusted R = 0 . 92 , P < 0 . 0005 ) of the variation in the level of IGF 1 in untreated Turner syndrome . ^^^ In controls IGFBP 3 , SHBG , BMI and age explained 74 % ( adjusted R = 0 . 86 , P < 0 . 0005 ) of the variation in IGF 1 , while GH status did not contribute at all . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Mean IGF 1 serum levels were 1 . 9 ( 0 . 7 , 2 . 4 , 2 . 5 ) SDS and mean IGFBP 3 serum levels 1 . 2 ( 1 . 1 , 1 . 2 , 1 . 2 ) SDS . ^^^ Therapy with rhGH was effective in causing catch up growth of the three children with an initial mean growth rate of + 2 . 9 ( + 1 . 7 , + 2 . 1 , + 5 . 0 ) SDS , and normalization of IGF 1 ( mean : 0 . 66 SDS : 1 . 8 , 1 . 2 , + 1 . 1 SDS ) and IGFBP 3 serum levels ( mean : + 0 . 81 SDS : 0 . 2 , + 0 . 8 , + 1 . 8 SDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patient serum contained low levels of IGF 1 , IGFBP 3 and ALS , while the concentrations of IGFBP 2 and IGFBP 6 were markedly elevated . ^^^ Northern blot analysis of total mRNA isolated from the tumour demonstrated high expression of the IGF 2 gene and abundant 1 . 1 kb IGFBP 6 transcript , while the genes encoding IGFBP 3 , 4 and 5 were only weakly expressed and mRNA of IGFBP 1 , 2 and IGF 1 could not be detected . mRNAs for the IGF type 2 receptor could be easily demonstrated , whereas those for the insulin and IGF type 1 receptor were hardly detectable . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN , PATIENTS AND MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , osteocalcin ( OST ) , bone specific alkaline phosphatase ( BAP ) , urinary deoxypyridinoline ( DPYD ) and N telopeptide of type 1 collagen ( NTX ) were measured in 31 premenopausal women aged 31 43 and 65 postmenopausal women aged 47 60 : this latter group comprised 30 normal healthy women and 35 osteoporotic women . ^^^ RESULTS : Compared with premenopausal women or normal postmenopausal women , serum IGF 1 and IGFBP 3 levels were significantly lower in osteoporotic postmenopausal women while no significant differences in serum levels of IGF 2 , IGFBP 1 and IGFBP 2 were observed . ^^^ However , serum IGF 1 and IGFBP 3 correlated positively with BMD of the lumbar spine and / or Ward ' s triangle even if age , BMI and menopause duration were taken into account in a multiple regression analysis model . ^^^ CONCLUSIONS : Circulating IGF 1 and IGFBP 3 may be involved in the mechanism of bone loss in postmenopausal women under the age of 60 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The growth hormone ( GH ) dependent growth factors insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) may be superior to provocative GH testing in diagnosing GH deficiency ( GHD ) in children . ^^^ Serum IGF 1 and IGFBP 3 concentrations do not accurately predict growth hormone deficiency in children with brain tumours . ^^^ In adults with brain tumours ( BT ) and GHD , however , provocative GH testing more accurately reflects GHD than either IGF 1 or IGFBP 3 . ^^^ PATIENTS : 72 children ( 51 M , 21 F ) with BT diagnosed with GHD by clinical and auxological criteria , including provocative GH testing , in whom pre GH treatment IGF 1 and IGFBP 3 levels were obtained . ^^^ MEASUREMENTS : Auxological data , including height , weight , growth velocity , and pubertal stage ; and biochemical data , including GH response to provocative GH testing and pre GH treatment serum IGF 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We suggest that this effect may be mediated by increased IGF 1 in ad libitum fed animals , whereas food restriction may block this effect by resulting in low levels of circulating IGF 1 and by attenuating the pregnancy associated increase in IGF binding protein 3 protease activity and , thereby , further compromise IGF bioavailability . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , IGF 1 and free IGF 1 were analyzed by immunoradiometric assay ( IRMA ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) by Western immunoblot . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Continuous s . c . infusion rather than twice daily injections of IGF 1 more effectively increases serum IGF binding protein 3 in female monkeys . ^^^ Clinical studies suggest that circulating concentrations of insulin like growth factor binding protein 3 ( IGFBP 3 ) are decreased or unaffected by IGF 1 administration , whereas acute increases in IGF 1 may increase serum IGFBP 1 . ^^^ However , studies in monkeys indicate that acute or continuous infusion of IGF 1 effectively increases serum IGFBP 3 . ^^^ Serum IGFBP 3 followed the pattern of IGF 1 , with concentrations increased by day 1 during constant infusion and by day 2 during intermittent injections . ^^^ Immunoblotting revealed that the constant infusion of IGF 1 resulted in quantitatively more lower molecular mass fragments of IGFBP 3 than were observed during baseline or intermittent injections . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The younger group demonstrated higher total and free testosterone and IGF 1 than the older men , training induced increases in free testosterone at rest and with exercise , and increases in resting IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After controlling for sexual maturation and fat mass , the serum concentrations of total IGF 1 , bound IGF 1 , and IGFBP 3 in the white girls became significantly higher than those in the African American girls . ^^^ The higher concentrations of total IGF 1 in the white girls were due to a proportional increase in the concentrations of bound IGF 1 that coincided with a similar increase in serum IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There were no differences between the PCOS subjects and the normoovulatory group for total IGF 1 , IGF 2 , or IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum 25 hydroxyvitamin D , 1 , 25 dihydroxyvitamin D , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 were analyzed at diagnosis and at 1 yr follow up . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Finally , treatment with hGH ( 2 IU , s . c . daily ) , over 4 days , led to a clear cut increase in plasma IGF 1 and IGFBP 3 levels in patients with FM . ^^^ Furthermore , our finding of increased IGF 1 and IGFBP 3 levels after GH treatment , over 4 days , opens up the possibility of testing the therapeutic potential of hGH in patients with FM . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There were no correlations between these plasma cytokine levels or monocyte cytokine production and parameters of body composition , lipid profile , or IGF 1 and IGF binding protein 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were reduced in the plasma of homozygotes of both sexes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Reference values for IGF 1 , IGFBP 1 , IGFBP 3 and osteocalcin in healthy children in Zaragoza ] . ^^^ Immunoradiometric assays were used to determine basal serum IGF 1 , IGFBP 1 , IGFBP 3 and osteocalcin concentrations . ^^^ IGF 1 , IGFBP 1 , IGFBP 3 and osteocalcin concentrations differ during the pubertal period according to age . ^^^ There are differences in IGF 1 , IGFBP 3 and osteocalcin levels between prepuberty and puberty and differences in IGF 1 and osteocalcin levels among the pubertal stages . ^^^ Sex did not influence IGF 1 or IGFBP 1 concentrations and there were punctual differences in IGFBP 3 and osteocalcin levels between girls and boys . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present data support the hypothesis that the combination of TNFalpha and IFN gamma with retinoic acid exert their anti proliferative effect on HSG cells by reducing the mitogenic effect of IGF 1 due to a shift in IGF 1 from the free to the IGFBP 3 bound form . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PURPOSE : To evaluate and quantify the association between consumption of specific food groups / macronutrients and concentrations of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ SUBJECTS AND METHODS : Data from a comprehensive food frequency questionnaire administered to 115 healthy subjects were used to study cross sectionally the relationship between nutritional factors and circulating IGF 1 and IGFBP 3 concentrations . ^^^ CONCLUSION : Serum IGF 1 and / or IGFBP 3 concentrations are associated with red meat , carbohydrate intake , and fat intake and , thus , may mediate the effect of these dietary factors on the pathogenesis of several disease states . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 increased IGFBP 3 , IGFBP 2 and decreased IGFBP 4 , while TGF beta 1 decreased IGFBP 3 and apparently increased IGFBP 4 . ^^^ However , rhIGFBP 3 alone had no effect on GENC , but after 48 h pre incubation the IGF 1 stimulated GENC growth was increased , suggesting that IGFBP 3 could modulate the IGF 1 induced GENC proliferation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition to measuring IGF 1 , IGF 2 and IGFBP 3 , serum IGFBP 1 , 2 , 4 and 5 were measured . ^^^ Serum IGF 1 ( 25 microg / l ) , IGF 2 ( 194 microg / l ) , IGFBP 3 ( 0 . 4 mg / l ) , and IGFBP 5 ( 87 microg / l ) levels were low compared to age matched prepubertal children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The ability of IGF binding proteins ( IGFBPs ) such as IGFBP 3 to reduce the rate of IGF 1 clearance from wounds suggests that IGFBP 3 might bind directly to fibrinogen / fibrin . ^^^ IGF 1 does not compete for IGFBP 3 binding ; instead , IGF 1 binds immobilized IGFBP 3 . fibrinogen and IGFBP 3 . fibrin complexes with affinity similar to that of IGF 1 for the type 1 IGF receptor . ^^^ IGFBP 3 also binds specifically to native fibrin clots , and addition of exogenous IGFBP 3 increases IGF 1 binding . ^^^ These studies suggest that IGF 1 can concentrate at wound sites by binding to fibrin immobilized IGFBP 3 , and that the lower IGF affinity of fibrin bound IGFBP 3 allows IGF 1 release to type 1 IGF receptors of stromal cells migrating into the fibrin clot . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Testosterone was administered to peripubertal rats and the responses of mRNA of GH receptor , IGF 1 , IGF 1 receptor and IGF binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) as well as circulating IGF 1 were evaluated in two time related models : over 12 h after a single injection ( short term study ) and 10 days after continuous administration ( long term study ) . ^^^ These data suggest that the effect of testosterone on growth ( as assessed by weight gain ) is small and is not mediated by changes in hepatic gene expression of IGF 1 , IGF 1 receptor , IGFBP 1 , IGFBP 3 or circulating IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and free IGF 1 concentrations increased , whereas IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) were unchanged during treatment . ^^^ There was no correlation between absorption or terminal half life of IGF 1 and IGFBP 3 or ALS , but negative correlations with IGF 1 clearance ( CL / F ) and volume of distribution ( V / F ) . ^^^ Positive correlations between both IGFBP 3 and ALS and IGF 1 maximal concentration ( C ( max ) ) and time of C ( max ) ( T ( max ) ) were observed . ^^^ Additionally , IGFBP 3 and ALS concentrations modulate the peak concentrations of IGF 1 achieved and correlate reciprocally with its V / F and CL / F , underscoring the critical importance of binding proteins in modulating the bioavailability of IGF 1 in vivo in humans . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Associations of IGF 1 , IGFBP 1 and IGFBP 3 on intrauterine growth and early catch up growth . ^^^ Fetal cord blood IGF 1 , IGFBP 1 and IGFBP 3 levels of appropriate for gestational age ( AGA ) and intrauterine growth retardation ( IUGR ) babies are studied and followed up for 6 9 months , reevaluated for anthropometric measures and the effects of IGF 1 , IGFBP 1 and IGFBP 3 on fetal growth and early catch up growth is investigated . 23 AGA and 21 IUGR babies , totally 44 newborns , were included in the study protocol . ^^^ IGF 1 and IGFBP 3 levels were found to be high in AGAs with respect to IUGR babies and IGFBP 1 is found to be high in IUGR with respect to AGAs . ^^^ A good correlation was found between birth weight , postnatal weight and postnatal height and IGF 1 and IGFBP 3 levels ( p < 0 . 05 ) but not with IGFBP 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate serum and follicular fluid ( FF ) insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) behavior in superstimulated cycles in patients with polycystic ovary syndrome ( PCOS ) . ^^^ MAIN OUTCOME MEASURE ( S ) : E 2 , GH , IGF 1 , and IGFBP 3 assayed by RIA and immunoradiometric assay . ^^^ CONCLUSION ( S ) : The apparent failure of IGFBP 3 reduction in patients with PCOS alters IGF 1 bioavailability . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were taken for analysis of leptin , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and 24 h GH secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recombinant human GH treatment ( 0 . 15 IU / kg 10 day ) for 1 week increased serum total IGF 1 , IGFBP 3 , and ALS , exaggerating the responses to exercise . ^^^ Total IGF 1 , IGFBP 3 , and ALS returned to baseline over 3 4 days . ^^^ In summary , 1 ) acute exercise transiently increased all components of the IGF 1 ternary complex , possibly due to mobilization of preformed intact complexes ; 2 ) GH pretreatment augmented the exercise induced changes in ternary complexes ; 3 ) postexercise IGFBP 1 increments may protect against delayed onset hypoglycemia ; 4 ) serum total IGF 1 , IGFBP 3 , and ALS may be suitable markers of GH abuse ; and 5 ) differences in disappearance times altered the sensitivity of each marker for detecting GH abuse . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Compared with controls , estrogen treated monkeys had 2 fold higher SF levels of IGF 1 ( P < 0 . 001 ) , 1 . 7 fold higher IGF 2 ( P < 0 . 006 ) , 5 . 9 fold higher IGFBP 1 ( P < 0 . 02 ) , and 2 . 5 fold higher IGFBP 3 ( P < 0 . 001 ) . ^^^ Estrogen plus progesterone treated monkeys had SF levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 that were intermediate between the levels in the control and estrogen groups , except that the level of IGFBP 3 was significantly greater than that in the control group ( P < 0 . 001 ) . ^^^ Treatment group did not affect the serum levels of IGF 1 and IL 6 , but IGF 2 and IGFBP 3 were increased by 1 . 6 and 1 . 8 fold , respectively , in the estrogen group ( P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PROCEDURE : Serum levels of human intact osteocalcin , type 1 collagen carboxyterminal propeptide ( PICP ) , type 1 collagen carboxyterminal telopeptide ( ICTP ) , 25 hydroxyvitamin D [ 25 ( OH ) D ] , 1 , 25 dihydroxyvitamin D [ 1 , 25 ( OH ) ( 2 ) D ] , intact parathyroid hormone , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , alkaline phosphatase , calcium , and phosphate were analyzed in 22 children with acute lymphoblastic leukemia and in 26 children with other malignancies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Under basal conditions , fasting serum concentrations of epinephrine , norepinephrine , cortisol , glucagon , insulin , IGF 1 , and IGFBP 3 were not significantly different among the four groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine if insulin like growth factor 1 ( IGF 1 ) is associated with the increase in LVM , we measured IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels in 19 postmenopausal women before and after 8 weeks of oral treatment with MPA 5 mg / d . ^^^ Changes in IGF 1 , IGFBP 3 , and LVM from baseline were analyzed by paired ttest . ^^^ IGF 1 increased 17 % with MPA ( P = . 008 ) , whereas IGFBP 3 did not change . ^^^ The IGF 1 / IGFBP 3 ratio increased 16 . 8 % ( P = . 0003 ) . ^^^ Regression analysis of LVM with IGF 1 , IGFBP 3 , and the IGF 1 / IGFBP 3 ratio suggested that IGF 1 during MPA therapy explains 2 . 4 % and the IGF 1 / IGFBP 3 ratio explains 3 . 2 % of the variation in LVM . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The secretion of hormones , IGF 1 and IGFBP 3 into the culture medium was measured using RIA / IRMA . ^^^ It was found that the addition of GH to the culture medium strongly ( P < 0 . 05 ) stimulated IGF 1 ( at a concentration of 0 . 001 10 microgram GH / ml medium ) , IGFBP 3 ( 0 . 001 1 microgram GH / ml ) and oxytocin ( 0 . 01 10 microgram GH / ml ) secretion . ^^^ KT 5720 given alone significantly ( P < 0 . 05 ) inhibited the secretion of IGFBP 3 , but not that of IGF 1 or progesterone . ^^^ KT 5720 and Rp cAMPS fully or partially prevented the GH effect on IGF 1 , IGFBP 3 , oxytocin , progesterone , estradiol and apoptosis . ^^^ These observations suggest the involvement of GH and a cAMP / PKA dependent intracellular cascade in the control of IGF 1 , IGFBP 3 , oxytocin , progesterone , estradiol , cAMP and apoptosis in bovine ovarian granulosa cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in healthy Thai children and adolescents : relation to height , weight , and body mass index . ^^^ The objective of this study was to evaluate the correlation between serum concentrations of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) and growth parameters ( height , weight , and body mass index ) in 260 healthy children and adolescents aged 5 20 years . ^^^ In group 1 , the serum concentrations of IGF 1 and IGFBP 3 were significantly positive correlated with all growth parameters . ^^^ In group 2 , although the correlation was insignificant , the concentrations of IGF 1 and IGFBP 3 seemed to be greater in individuals who were relatively taller and had lean body mass than those who were relatively short and over average body mass . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The high nutritional status of pigs in the early phases of growth diminished the postnatal changes in IGF 1 and IGFBP 2 , but not IGF 2 or IGFBP 3 . ^^^ Overall , data demonstrate a developmental regulation of the ST / IGF system , with pST challenges altering circulating concentrations of IGF 1 , IGFBP 3 , and IGFBP 2 coincident with changes in amino acid metabolism . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The purpose of this study was to determine the anabolic effects of recombinant human insulin like growth factor 1 ( rhIGF 1 ) complexed with its principal binding protein IGF 1 binding protein 3 ( IGFBP 3 ) in severely burned adults . ^^^ RESULTS : Serum IGF 1 and IGFBP 3 levels increased with all doses , but no incremental increases were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) 1 , IGF 1 receptor , and IGF binding protein 3 messenger ribonucleic acids and protein in corpora lutea from prostaglandin F ( 2alpha ) treated gilts . ^^^ By slot blot analysis , steady state levels of IGF 1 and IGFBP 3 mRNA were not different in PGF ( 2alpha ) treated vs . control animals ; however , IGF IR mRNA was increased in treated animals on Day 9 . ^^^ In conclusion , PGF ( 2alpha ) induced premature luteolysis was associated with an increase in steady state levels of IGF IR mRNA , but it did not appear to be linked to changes in mRNA levels for IGF 1 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 4 females we observed plasmatic IGF 1 and IGFBP 3 concentrations significantly reduced either for chronological age and pubertal stage ; this finding emerged in only 1 male . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : At baseline and at the end of the follow up , insulin mediated glucose uptake was assessed by euglycemic glucose clamp and plasma total IGF 1 and IGF binding protein 3 ( IGF BP 3 ) in each subject , and concentrations were determined . ^^^ Low baseline fasting plasma IGF 1 concentration ( RR = 1 . 5 , 95 % CI = 1 . 3 1 . 7 ) and plasma IGF 1 / IGFBP 3 molar ratio ( RR = 1 . 4 , 95 % CI = 1 . 3 1 . 8 ) predicted a decline in WBGD . ^^^ Fasting plasma total IGF 1 concentrations ( 84+ / 56 vs 63+ / 44 microg / L , P < . 040 ) , plasma IGF 1 / IGF BP 3 molar ratio ( 0 . 13+ / 0 . 05 vs 0 . 10+ / 0 . 03 P < . 050 ) , and WBGD ( 34 . 8+ / 5 . 0 vs 23 . 1+ / 4 . 6 micromol / kg 10 min , P < . 010 ) were more elevated at baseline than at the end of the follow up . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Before surgery , all 13 non treated patients had elevated serum levels of IGFBP 3 as well as total and free IGF 1 . ^^^ CONCLUSIONS : These data confirm that IGFBP 3 is a better indicator of acromegalic activity than either total or free IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this paper , we show that the inhibition of GnRH secretion by 10 ( 10 ) M of IGF 1 at 50 days is prevented in a dose dependent manner by 0 . 3 to 3 nM of IGF BP 2 as well as IGF BP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We hypothesize that plasma IGF 1 and serum IGFBP 3 concentrations in breast cancer patients may provide useful information on the progression of their disease , and determine the probability of recurrence and survival . ^^^ Plasma IGF 1 and serum IGFBP 3 were correlated with tumor histopathology , menopausal status , treatment modality , recurrence rates , and probability of survival . ^^^ Plasma IGF 1 and serum IGFBP 3 were measured by radioimmunoassay . ^^^ Our studies show that breast cancer patients have elevated plasma IGF 1 and serum IGFBP 3 levels . ^^^ IGF 1 and IGFBP 3 increased with tumor size ( T 4 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , 5alpha DHT decreased IGFBP 4 mRNA and protein levels by 2 and 4 fold , respectively , and increased IGFBP 2 and 3 mRNA and protein levels by 6 and 7 fold ( for mRNA ) and 3 and 5 fold ( for protein ) , respectively . hFOB / AR 6 cells expressed the type 1 IGF receptor , but this was not regulated by 5alphaDHT . 5alphaDHT and IGFBP 3 specifically increased hFOB / AR 6 cell proliferation , and a monoclonal antibody specific for IGF 1 blocked this effect . ^^^ Thus , androgens increase the expression of IGF 1 , IGFBP 2 , and IGFBP 3 , but decrease levels of the inhibitory IGFBP 4 in an androgen responsive human osteoblastic cell line . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Insulin like growth factor 1 ( IGF 1 ) is a potent mitogen for normal and neoplastic cells , whereas IGF binding protein 3 ( IGFBP 3 ) inhibits cell growth in many experimental systems . ^^^ We therefore examined associations of plasma levels of IGF 1 and IGFBP 3 with the risk of colorectal cancer in a prospective case control study nested in the Physicians ' Health Study . ^^^ IGF 1 , IGF 2 , and IGFBP 3 were assayed among 193 men later diagnosed with colorectal cancer during 14 years of follow up and among 318 age and smoking matched control subjects . ^^^ RESULTS : IGFBP 3 levels correlated with IGF 1 levels ( r= . 64 ) and with IGF 2 levels ( r= . 90 ) . ^^^ After controlling for IGFBP 3 , age , smoking , body mass index ( weight in kg / [ height in m ] 2 ) , and alcohol intake , men in the highest quintile for IGF 1 had an increased risk of colorectal cancer compared with men in the lowest quintile ( relative risk [ RR ] =2 . 51 ; 95 % confidence interval [ CI ] =1 . 15 5 . 46 ; P for trend = . 02 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To examine whether GH / IGF 1 axis function in the elderly is related to the lipid profile independently of body fat , we evaluated GH secretion , serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels , adiposity via the body mass index ( BMI ) , waist to hip ratio ( WHR ) , dual energy 10 ray absorptiometry ( DEXA ) , and magnetic resonance imaging ( MRI ) , and circulating lipids in 101 healthy subjects older than 65 years . ^^^ Age , IGF 1 , and IGFBP 3 were not significantly related to any body fat or lipid measures , except for a positive correlation of IGF 1 with triglycerides in men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Non diabetic obese subjects had increased free IGF 1 and 2 , total IGF 2 , IGFBP 3 and GHBP , reduced IGFBP 1 and 2 ( p < 0 . 05 ) , but normal total IGF 1 , when compared to lean subjects . ^^^ Also IGFBP 1 and total IGF 1 were normal in obese Type 2 diabetics , whereas free and total IGF 2 and IGFBP 3 remained elevated to a similar extent as in simple obesity ( p < 0 . 05 ) . ^^^ In Type 1 diabetics total IGF 1 and 2 , and IGFBP 3 were normal . ^^^ Many of these abnormalities were unaffected by the concomitant presence of Type 2 diabetes ( total IGF 1 , free and total IGF 2 and IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Specifically the reduced amount of GH released in pulses is found to be related to low circulating levels of IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) , which suggests that a relative hyposomatotropism may participate in the pathogenesis of the wasting syndrome distinctively in the chronic phase of critical illness . ^^^ The relative hyposomatotropism seems at least in part of hypothalamic origin since the whole somatotropic axis has been found to be very responsive to continuous infusion of GH releasing peptide ( GHRP ) , administered alone or in combination with GH releasing hormone ( GHRH ) , as evidenced by reactivated pulsatile GH secretion followed by substantial increases in circulating levels of IGF 1 , IGFBP 3 and ALS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Because determinants of tissue IGF bioactivity appear to be regulated in parallel with circulating IGF 1 level , it is reasonable to hypothesize that the substantial intraindividual variability in circulating levels of IGF 1 and IGFBP 3 may be important in determining risk of some cancers . ^^^ In recent epidemiologic studies , relatively high plasma IGF 1 and low IGFBP 3 levels have been independently associated with greater risk of prostate cancer in men , breast cancer among premenopausal women , and colorectal adenoma and cancer in men and women and possibly lung cancer . ^^^ In general , two to fourfold elevated risks have been observed for prostate cancer in men in the top quartile of IGF 1 relative to those in the bottom quartile , and low levels of IGFBP 3 were associated with an approximate doubling of risk . ^^^ Recent data also indicate that high IGF 1 and low IGFBP 3 increase risk of colorectal cancer and large or villous adenomas . ^^^ Of note , for colorectal neoplasia , fourfold elevated risks were observed in men and women with low IGFBP 3 , whereas high IGF 1 was associated with a doubling of risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting serum levels of IGFBP 1 , IGFBP 2 , IGFBP 3 , IGF 1 and free IGF 1 were determined using appropriate immunoenzymatic , radioimmuno or immunoradiometric assays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND DESIGN : We measured serum levels of insulin , IGF 1 , IGFBP 1 , IGFBP 3 and IGF bioactivity in 77 fasting subjects ( 43 male ) mean age 9 . 6 years ( range 2 . 99 17 . 98 years ) . ^^^ Insulin levels were also low and correlated with IGF 1 and IGFBP 3 ( r = 0 . 42 , P = 0 . 0004 , and r = 0 . 45 , P = 0 . 0002 , respectively ) whereas levels of IGFBP 1 were inversely related to those of IGF 1 and insulin ( r = 0 . 43 , P = 0 . 0004 , r = 0 . 52 , P < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No differences were seen between the two uninephrectomized groups with respect to body weight , food intake , blood glucose , serum GH , IGF 1 , or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thyroid hormone modulates insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 , without mediation by growth hormone , in patients with autoimmune thyroid diseases . ^^^ The expression and synthesis of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) are regulated by various hormones and nutritional conditions . ^^^ We evaluated the effects of thyroid hormones on serum levels of IGF 1 and IGFBP 3 levels in patients with autoimmune thyroid diseases including 54 patients with Graves ' disease and 17 patients with Hashimoto ' s thyroiditis , and in 32 healthy age matched control subjects . ^^^ Serum levels of growth hormone ( GH ) , IGF 1 and IGFBP 3 were determined by radioimmunoassay . ^^^ The serum levels of IGF 1 and IGFBP 3 showed a significant positive correlation in the patients ( R=0 . 616 , P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of albumin , total protein , transferrin , leptin IGF 1 , IGFBP 3 and the protein catabolic rate remained unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of recombinant human growth hormone and insulin like growth factor 1 administration on IGF 1 and IGF binding protein 3 levels in brain injury . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also measured the serum levels of insulin , total and free IGF 1 , and IGFBP 3 to investigate their relationships to the IGFBP 1 phosphorylation status in obese subjects . ^^^ The circulating free IGF 1 level was significantly higher in the obese children than in the controls ( P < 0 . 05 ) , while the serum levels of insulin , total IGF 1 and IGFBP 3 were not significantly different between the two groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The diagnostic sensitivity ( % ) of IGF 1 , IGF I / GHBP , IGF I / IGFBP 3 , and of the combination of IGF 1 plus IGF 2 ( both low or one normal and one low ) , was higher in female patients than in male ( IGF 1 : 57 . 8 vs 22 . 0 , P < 0 . 0001 ; IGF I / GHBP : 84 . 2 vs 48 . 8 , P=0 . 002 ; IGF I / IGFBP 3 : 36 . 8 vs 7 . 3 P=0 . 001 ; IGF I+IGF 2 : 77 . 8 vs 52 . 6 , P=0 . 01 ) . ^^^ Forward regression analyses revealed that IGFBP 3 was a significant predictor of IGF 1 levels in both patients and healthy subjects . ^^^ CONCLUSION : ( 1 ) Levels of IGF 1 , and of IGF I / IGFBP 3 and IGF I / GHBP ratios are lower in females compared with male adult GHD patients . ( 2 ) IGF I / GHBP has a high diagnostic sensitivity of adult GHD , in particular in women . ( 3 ) We hypothesize that the gender difference in IGF 1 levels among adult GHD patients are causally related to the very low androgen levels observed among females . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , but not [ LR 3 ] IGF 1 , reduced the proportion of phosphorylated IGFBP 3 in Hs578T conditioned medium , consistent with increased release of non phosphorylated , cell associated IGFBP 3 . ^^^ By contrast , IGFBP 3 phosphorylation in T47D ( BP 3 ) cells was not affected by retinoic acid or IGF 1 , but appeared slightly increased by estradiol . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We , therefore , investigated whether plasma levels of IGF 1 and IGFBP 3 vary by race in United States men selected from among members of the Health Professionals Follow up Study who were 47 78 years old in 1993 1995 when they provided blood ( n = 18 , 000 ) . ^^^ IGF 1 and IGFBP 3 concentrations were determined by ELISA . ^^^ Median molar IGF 1 : IGFBP 3 ratio was greatest in Caucasians and lowest in Asians . ^^^ The lower IGF 1 blood levels relative to IGFBP 3 levels among Asian men are consistent with their lower prostate cancer incidence . ^^^ Although differences in circulating IGF 1 do not seem to account for the greater prostate cancer risk among African American men , their absolute lower levels of IGFBP 3 may be contributory . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : GH treatment caused a rapid ( within 2 weeks ) increase in serum levels of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) which was sustained throughout the study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The Ati Negritos showed lower growth hormone binding protein ( GHBP ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) , zinc , albumin , ferritin , iron , iron saturation and much higher insulin like growth factor binding protein 2 ( IGFBP 2 ) and plasma transferrin concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The evaluation of IGF 1 , IGFBP 3 and even of spontaneous GH secretion do not establish the diagnosis of adult GHD . ^^^ The aim of our study was to determine the effects of 36 h fasting on 8 h diurnal GH , insulin and glucose levels as well as on basal IGF 1 , IGFBP 3 , acid labile subunit ( ALS ) , IGFBP 1 , GHBP and free fatty acid ( FFA ) levels . ^^^ STUDY DESIGN : In all subjects we studied the effects of 36 h fasting on 8 h daytime GH , insulin and glucose levels ( assay every 30 min from 0800 h to 1600 h ) as well as on basal IGF 1 , IGFBP 3 , ALS , IGFBP 1 , GHBP and FFA levels . ^^^ RESULTS : Before fasting , basal mean IGF 1 , IGFBP 3 and ALS levels in GHD were lower ( P < 0 . 0001 ) than in NS . ^^^ After fasting , both in GHD and NS basal IGF 1 , IGFBP 3 , ALS and GHBP levels did not change significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND DESIGN : Eleven prepubertal children ( 3 females , 8 males ) with GHIS ; basal GH > 2 . 5 microg / l , IGF 1 < 50 microg / l , IGFBP 3 < 2SD ; were treated with IGF 1 80 microg / kg twice daily in a multi centre study . ^^^ Pre treatment height SDS at the start of IGF 1 therapy correlated positively with pretreatment serum IGFBP 3 SDS levels ( r = 0 . 85 ; P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^ In pooled data from the normal and osteoporotic populations , age adjusted multiple regression models based on IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 were found to be highly predictive of serum OC ( R 2 = 19 % , p < 0 . 001 ) and BMD of femoral neck ( R 2 = 49 % , p < 0 . 0001 ) , consistent with an effect of the anabolic IGF components on overall bone formation rate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After 1 week off GH treatment , significantly lower levels of IGF 1 , IGF 2 , IGFBP 3 , and ALS were recorded ( P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGFBP 3 , and acid labile subunit ( ALS ) levels were low in patients compared with controls , with male patients revealing lower IGF 1 ( P = 0 . 01 ) and ALS ( P = 0 . 005 ) concentrations than female patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To clarify the effects of nutritional status on bone metabolism , we examined the relationship between serum levels of nutritional indicators [ insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 ] and markers for bone metabolism [ serum osteocalcin and urinary excretion of C terminal telopeptide of collagen type 1 ( CrossLaps ) ] in 45 AN out patients , including 8 severely malnourished patients who required hospitalization and 4 hyperalimentation ( IVH ) . ^^^ Compared to healthy subjects , serum IGF 1 and IGFBP 3 were lower , whereas IGFBP 2 was higher in out patients who had a body mass index ( BMI ) less than 16 . 5 kg / m2 . ^^^ Serum levels of osteocalcin correlated positively with BMI ( r = 0 . 512 ; P < 0 . 0001 ) , IGF 1 ( r = 0 . 558 ; P < 0 . 0001 ) , and IGFBP 3 ( r = 0 . 369 ; P < 0 . 001 ) in AN out patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 , IGFBP 3 and osteocalcin increased over time . ^^^ CONCLUSION : Changes in circulating IGFBP 2 and IGFBP 3 paralleled the changes reported in non steroid treated infants ; however , it remains uncertain whether the natural rise in IGF 1 was suppressed by DEX treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We found severalfold increased serum levels of IGFBP 3 ( 2 fold ) , IGFBP 4 ( 5 fold ) and slightly increased IGF 2 levels in ESRF patients as well as a 2 . 6 fold increase in free IGF 1 in CRF patients with PTH < 100 pg / ml . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , the plasma IGF 1 and insulin status are markedly , albeit transiently , decreased in calves fed colostrum with a delay of 12 to 24 h , and the decreased concentrations of plasma IGF 1 were associated with decreased IGFBP 3 / IGFBP 2 ratios . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the growth hormone ( GH ) response to provocation by clonidine and glucagon , measured the circulating concentrations of insulin , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and ferritin , and evaluated the spontaneous nocturnal ( 12 h ) GH secretion in prepubertal patients with thalassaemia and age matched children with constitutional short stature ( CSS ) ( height SDS < 2 , but normal GH response to provocation ) . ^^^ They had significantly decreased circulating concentrations of IGF 1 and IGFBP 3 ( 68 . 5 + / 19 ng / ml and 1 . 22 + / 0 . 27 mg / l respectively ) compared to controls ( 153 + / 42 ng / ml and 2 . 16 + / 0 . 37 mg / l respectively ) . ^^^ Those with a normal GH response after provocation also had significantly lower IGF 1 and IGFBP 3 concentrations than controls . ^^^ Serum ferritin concentration was correlated significantly with the circulating IGF 1 ( r = 0 . 47 , p < 0 . 01 ) and IGFBP 3 ( r = 0 . 43 , p < 0 . 01 ) concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Usefulness of the measurement of insulin like growth factor ( IGF 1 ) and IGF 1 binding protein 3 ( IGFBP 3 ) for the diagnosis of growth hormone ( GH ) deficiency in children ] . ^^^ AIM : To evaluate the usefulness of IGF 1 and IGFBP 3 for the diagnosis of GHD in prepuberal children . ^^^ MATERIAL AND METHODS : We measured IGF 1 and IGFBP 3 in three group of subjects : 1 . ^^^ RESULTS : IGF 1 and IGFBP 3 were significantly lower in GHD than in NGHD and controls ( p < 0 . 001 ) , and in NGHD than in C ( p < 0 . 001 ) . ^^^ CONCLUSIONS : IGF 1 and IGFBP 3 are important tools for the diagnosis of GHD . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) is predominantly bound to IGF binding protein 3 ( IGFBP 3 ) , and free form of IGF 1 ( fIGF 1 ) may be bioactive in the circulation . ^^^ Proteolysis of IGFBP 3 , as reported in pregnant serum , results in the lowering of the affinity for IGF 1 , thereby increasing the ratio of fIGF 1 to total IGF 1 ( f / t IGF 1 ratio ) . ^^^ Here , proteolysis of IGFBP 3 measured by Western immunoblotting ( ages 4 25 years ; n=11 ) and f / t IGF 1 ratio measured by immunoradiometric assay ( ages 4 25 years ; n=10 ) were studied in patients with GHD , which is similar to GHRD in terms of lowered GH function . ^^^ No proteolysis of IGFBP 3 was observed during a 24 hour period in sera obtained every two hours from two patients with GHD . f / t IGF 1 ratio was not increased in plasma from the 10 patients with GHD . ^^^ Our data suggest that proteolysis of IGFBP 3 is independent of the GH IGF 1 axis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the effect of insulinlike growth factor 1 ( IGF 1 ) in combination with its principal binding protein ( IGFBP 3 ) on the hepatic acute phase response in severely burned children . ^^^ RESULTS : Serum IGF 1 levels in burned children given the IGF I / BP 3 complex increased from 113 + / 15 to 458 + / 40 ng / mL and IGFBP 3 levels increased from 1 . 8 + / 0 . 2 to 3 . 1 + / 0 . 3 ng / mL . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein ( IGFBP ) 3 bound IGF 1 and IGFBP 3 bound IGF 2 in growth hormone deficiency . ^^^ We have used an equation derived from the law of mass action to estimate serum IGFBP 3 bound IGF 1 and IGFBP 3 bound IGF 2 , as well as serum free IGF 1 and free IGF 2 , in 129 control children and adolescents ( 48 girls and 81 boys ) and in 13 patients with GHD . ^^^ Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^ In controls , serum total IGF 1 , total IGF 2 , IGFBP 3 , IGFBP 3 bound IGF 1 , IGFBP 3 bound IGF 2 and free IGF 1 increased linearly with age , from less than 1 to 15 years , in the two sexes . ^^^ In the 13 patients with GHD , mean ( + / SD ) SDS of serum IGFBP 3 bound IGF 1 was 2 . 89 + / 0 . 97 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The individual hemoglobin levels correlated with the GH parameters [ P = 0 . 035 for insulin like growth factor 1 ( IGF 1 ) and P = 0 . 002 for IGF binding protein 3 ] , and the mean corpuscular volume of red blood cell values correlated with fetal hemoglobin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Screening included serum assays for fasting insulin and glucose , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , free IGF 1 , inhibin B , leptin , and vascular endothelial growth factor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Spontaneous and phytohemagglutinin ( PHA ) stimulated interleukin ( IL ) 6 release by cultured peripheral blood mononuclear cells was related to height velocity , bone age , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) serum level standard deviation scores ( SDS ) of 32 children [ aged 91 ( median ; range 13 151 ) months ] with human immunodeficiency virus type 1 ( HIV 1 ) perinatal infection and severe disease . ^^^ Spontaneous and PHA stimulated IL 6 release inversely correlated with height velocity , bone age , IGF 1 , and IGFBP 3 SDS . ^^^ Ten children with height velocity SDS < / = 2 , compared to 22 children with height velocity SDS > 2 , showed higher spontaneous and PHA stimulated IL 6 release and lower IGF 1 and IGFBP 3 SDS ( irrespective of CD 4 positive T lymphocyte counts , viral load , liver disease , or nutrition status ) . ^^^ IL 6 overproduction may be a mechanism of IGF 1 and IGFBP 3 down regulation and impaired linear growth in children with perinatal HIV 1 infection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Diagnostic efficiency of serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGF I / IGFBP 3 molar ratio and urinary GH measurements in the diagnosis of adult GH deficiency : importance of an appropriate reference population . ^^^ OBJECTIVE : To analyse the diagnostic role of serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGF I / IGFBP 3 molar ratio and urinary GH ( uGH ) excretion in adult GH deficiency ( GHD ) . ^^^ METHODS : IGF 1 and IGFBP 3 were measured by RIA ( Nichols ) and results expressed as standard deviation ( s . d . ) scores from our reference population and assay normative data ( s . d . score Nichols ) . uGH was measured by IRMA . ^^^ RESULTS : Within the control group , IGF 1 , IGFBP 3 , IGF I / IGFBP 3 ratio standardisation regarding our control population and IGF 1 with respect to the assay normative data resulted in disappearance of age related differences . ^^^ Greatest diagnostic efficiency was for IGF 1 standardised with respect to our controls ( 97 . 2 % ) , followed by s . d . score IGFBP 3 ( 92 . 9 % ) . s . d . score IGF / IGFBP 3 ratio and uGH showed poor diagnostic efficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Expression of IGFBP 3 was elevated in tumors from WAP DES , but not bitransgenic mice , indicating an alteration in the p53 / IGF 1 axis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Levels of IGF 1 and IGFBP 3 , measured by enzyme linked immunosorbent assay , were significantly lower in preterm babies ( 35 . 3 + / 15 . 1 and 1025 . 6 + / 562 . 8 ng / mL , respectively ) than in term babies ( 61 . 6 + / 39 . 5 and 1252 . 6 + / 403 . 2 ng / mL , respectively ; P < 0 . 01 ) , but neither insulin nor GH levels , measured by radioimmunoassay , showed any significant difference between the two groups ( P > 0 . 05 ) . ^^^ Among term babies , IGF 1 and IGFBP 3 levels were significantly higher in the LGA group ( 96 . 1 + / 34 . 1 and 1544 . 7 + / 418 . 1 ng / mL , respectively ) than in the AGA group ( 56 . 4 + / 37 . 6 and 1212 . 8 + / 383 . 4 ng / mL , respectively ; P < 0 . 01 ) . ^^^ Levels of IGF 1 and IGFBP 3 showed significant correlation with birthweight and length , respectively ( P < 0 . 01 ) , although GH and insulin levels did not ( P > 0 . 05 ) . ^^^ There was a significant correlation between IGF 1 and IGFBP 3 levels ( P < 0 . 01 , r = 0 . 64 ) , but IGF 1 and IGFBP 3 levels showed no relationship with GH or insulin levels . ^^^ CONCLUSIONS : Our results suggest that fetal growth depends on fetal levels of IGF 1 and IGFBP 3 and maternal factors , not on insulin or GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) concentrations compared to stimulated growth hormone ( GH ) in the evaluation of children treated for malignancy . ^^^ OBJECTIVE : The aim of this investigation was to evaluate the utility of IGF 1 and IGFBP 3 determinations in screening for GH deficiency ( GHD ) in children previously submitted to treatment for childhood malignancy . ^^^ PATIENTS AND METHODS : We compared the GH responses to two pharmacological tests ( arginine and levo dopa ) with the IGF 1 and IGFBP 3 levels in 48 patients ( 29 boys ) who had undergone bone marrow transplantation ( BMT ) ( 36 patients ) or treatment for a solid cranial tumor ( 12 patients ) . ^^^ GH peak < 8 ng / ml in both tests ) , and only three ( 13 . 6 % ) of the GHD patients had concomitant low IGF 1 levels ( i . e . 2 SD below the normal mean ) and only one ( 4 . 5 % ) an abnormal IGFBP 3 value ( i . e . 2 SD below the normal mean ) . ^^^ Among the 26 children with normal GH secretion , 21 ( 80 . 8 % ) also showed normal IGF 1 and IGFBP 3 levels , three ( 11 . 5 % ) had a concomitant low IGF 1 value and two ( 7 . 7 % ) a concomitant low IGFBP 3 value . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 levels were significantly lower ( P < 0 . 05 ) , and insulin like growth factor binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) significantly higher than an age matched healthy normal group . ^^^ Resting IGF 1 levels were significantly ( P < 0 . 05 ) elevated ( 70 % ) by the resistance training whereas no significant changes occurred in IGFBP 1 and IGFBP 3 levels . ^^^ IGFBP 1 / IGF 1 and IGFBP 3 / IGF 1 ratios were significantly decreased ( approximately 50 % ) as a result of resistance training ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In adults , insulin like growth factor binding protein 3 ( IGFBP 3 ) is the main carrier protein for circulating insulin like growth factors ( IGFs ) ( IGF 1 and 2 ) . ^^^ In addition , increased endothelial IGFBP 3 may modulate the interaction of IGFs with IGF 1 receptors at the site of injury and / or act independently on endothelial cell growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and its binding protein , insulin like growth factor binding protein 3 , increased during bed rest , indicating the possibility of resistance to IGF 1 in bones under reduced mechanical stress and strain . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To modulate the hepatic acute phase response after a thermal injury by the administration of insulin like growth factor 1 ( IGF 1 ) in combination with its principal binding protein 3 ( IGFBP 3 ) . ^^^ Rats were killed on postburn days 1 , 2 , 5 , and 7 and serum glucose , electrolytes , acute phase reactant proteins , tumor necrosis factor alpha , interleukin 1 beta , interleukin 6 , and rat and human serum IGF 1 and IGFBP 3 were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) have previously been considered individually OBJECTIVE : To test the hypothesis that the combined analysis of IGF 1 and IGFBP 3 could act as a surrogate marker for the diagnosis of GHI . ^^^ Failure of IGF 1 and IGFBP 3 to diagnose growth hormone insufficiency . ^^^ DESIGN : Reference ranges for IGF 1 and IGFBP 3 were calculated using 521 normal individuals . ^^^ RESULTS : No significant difference was found between either the IGF 1 or IGFBP 3 standard deviation scores ( SDSs ) in children with and without GHI . ^^^ CONCLUSIONS : Neither IGF 1 nor IGFBP 3 alone is a marker for GHI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) can inhibit cell growth by directly interacting with cells , as well as by forming complexes with IGF 1 and IGF 2 that prevent their growth promoting activity . ^^^ Inhibition was abolished by co incubation of IGFBP 3 with a 20 % molar excess of Leu ( 60 ) IGF 1 , a biologically inactive IGF 1 analogue that binds to IGFBP 3 but not to IGF 1 receptors . ^^^ DNA synthesis was not inhibited by incubation with a preformed 1 : 1 molar complex of Leu ( 60 ) IGF 1 and IGFBP 3 , indicating that only free IGFBP 3 inhibits CCL 64 DNA synthesis . ^^^ Inhibition by IGFBP 3 is not due to the formation of biologically inactive complexes with free IGF , since endogenous IGFs could not be detected in CCL 64 conditioned media ; any IGFs that might have been present could only have existed in inactive complexes , since endogenous IGFBPs were present in excess ; and biologically active IGFs were not displaced from endogenous IGFBP complexes by Leu ( 60 ) IGF 1 . ^^^ This complex co migrated with a complex formed after incubation with ( 125 ) 1 labeled transforming growth factor beta ( TGF beta ) that has been designated the type 5 TGF beta receptor . ( 125 ) 1 IGFBP 3 binding to the thick similar 400 kDa receptor was inhibited by co incubation with unlabeled IGF 1 or Leu ( 60 ) IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In all subjects , free and total IGF 1 levels showed significant negative correlations with IGFBP 1 , IGFBP 2 , and IGFBP 4 ( that is , inhibitory IGF system components ) and significant positive correlations with IGFBP 3 and IGFBP 5 ( that is , stimulatory IGF system components ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The leptin concentrations were positively correlated with the BMI before ( p < 0 . 03 ) and after ( p < 0 . 04 ) testosterone , but not with the GH peak after stimulation , or with plasma testosterone , IGF 1 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated in a randomized double blind placebo controlled study the effects of zinc supplementation ( 2 mg / kg / day ) for 12 weeks on growth , serum insulin like growth factor 1 ( IGF 1 ) and insulin like factor binding protein 3 ( IGFBP 3 ) on 3 to 9 month old infants with nonorganic failure to thrive ( NOFTT ) . 25 infants completed the study , 14 received zinc supplementation ( group A ) , and 11 received placebo ( group B ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND / AIMS : In the circulation , insulin like growth factor 1 ( IGF 1 ) is bound in a trimeric complex of 150 kDa with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ Whereas circulating IGF 1 and IGFBP 3 are reported to be low in patients with chronic liver failure , the level of ALS has not been described in relation to hepatic dysfunction . ^^^ During a haemodynamic investigation , blood samples were collected from the hepatic vein and femoral artery , and the plasma concentrations of ALS , IGF 1 and IGFBP 3 were determined . ^^^ IGF 1 and IGFBP 3 were similarly decreased in the cirrhotic patients ( 51 % , p < 0 . 001 ) . ^^^ ALS correlated significantly with indicators of liver dysfunction , including the Child Turcotte score ( r= 0 . 69 , p < 0 . 0001 ) , IGF 1 ( r=0 . 82 , p < 0 . 0001 ) and IGFBP 3 ( r=0 . 74 , p < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , IGF 1 specifically upregulates IGFBP 3 synthesis in these cells . ^^^ To investigate this effect on cell growth and IGF 1 responsiveness , cell lines were developed that constitutively express IGFBP 3 . ^^^ In contrast , preincubation of Mock cells with exogenous IGFBP 3 did not enhance responsiveness or sensitivity to IGF 1 . ^^^ Basal DNA synthesis was unaffected by either an IGF neutralizing antibody or exogenous IGFBP 3 , indicating the differences observed between +BP3 and Mock cells were not attributable to sequestration of endogenous IGF 1 by IGFBP 3 . ^^^ DNA synthesis was also increased in +BP3 cells , compared to controls , in response to 5 microg / ml insulin and 2 . 5 ng / ml Long R ( 3 ) IGF 1 , indicating that the potentiated response did not require an interaction with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the SF , the following parameters were determined : Interleukin 1 beta , IL 6 , IL 8 , IL 1 RA , TNF alpha , Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 as well as total proteinase activity and total collagenase activity . ^^^ The best predictor for the SF effects on PG synthesis of exposed cartilage was the proportion of free IGF 1 ( r = 0 . 573 , p < 0 . 001 , Spearman rank correlation ) followed by the SF concentrations of IGF 1 ( with a positive sign ) , IGFBP 3 , IL 1 beta , and TNF alpha ( all with a negative sign ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In adult animals , most circulating insulin like growth factor 1 ( IGF 1 ) and IGF 2 is sequestered in a 150 kDa complex composed of 1 molecule each of IGF , IGF binding protein 3 or 5 , and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Western ligand blotting showed induction of the IGFBP 3 doublet as well as IGFBPs with molecular masses of 24 kDa , most probably IGFBP 4 , by human GH , IGF 1 alone , and IGF 1 in combination with IGFBP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 levels increased in the IGF 1 group along with increased hepatic IGFBP 1 and 3 mRNA levels . ^^^ In the EGF / IGF 1 administration group , serum IGF 1 and IGFBP 3 levels were significantly lowered when compared with the IGF 1 administration group . ^^^ We conclude that EGF administration reduced serum IGFBP 3 whereas IGF 1 administration increased the level of IGFBP 3 and IGF 1 and resulted in an increased body and kidney weight in adult female rats . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Advanced glycosylation end products ( AGEs ) , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in patients with type 2 diabetes mellitus . ^^^ BACKGROUND : Advanced glycosylation end product ( AGE ) formation is a major mechanism for the development of complications in diabetes , and the possible roles of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) are not clearly established . ^^^ METHODS : We examined the associations of AGEs , free IGF 1 and IGFBP 3 in Type 2 diabetes mellitus ( DM ) patients under diverse conditions . ^^^ Insulin and IGFBP 3 were measured by RIA and free IGF 1 with an IRMA method . ^^^ In the total group , free IGF 1 ( r = 0 . 25 , p = 0 . 008 ) , and IGFBP 3 ( r = 0 . 22 , p = 0 . 021 ) were associated with HbA ( 1c ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the effects of IGF 1 on primary uterine myometrial cell proliferation , and on IGFBP 3 and IGFBP 4 gene expression . ^^^ In conditioned media , IGF 1 increased IGFBP 3 accumulation by release of cell associated IGFBP 3 . ^^^ In cell free conditioned media IGF 1 protected IGFBP 3 and enhanced IGFBP 4 proteolysis . ^^^ Northern blot analysis indicated that IGF 1 increased IGFBP 4 mRNA accumulation by stabilizing the mRNA while IGFBP 3 gene expression was slightly decreased . ^^^ The results demonstrate that IGF 1 regulates IGFBP 4 post trancriptionally and post translationally , whereas IGFBP 3 is only affected post translationally . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 but not the IGF 1 variant long R ( 3 ) IGF 1 increases serum IGFBP 3 in adolescent monkeys . ^^^ In the first study , an s . c . injection of IGF 1 ( 110 mg / kg ; n = 6 ) significantly elevated serum IGFBP 3 concentrations through 7 h following treatment . ^^^ In contrast , serum IGFBP 3 decreased throughout the day following an injection of Long R ( 3 ) IGF 1 ( 110 mg / kg , s . c . , n = 5 ) . ^^^ In the second study , serum IGFBP 3 were compared between untreated control females ( n = 6 ) and females treated continuously by s . c . infusion with either Long R ( 3 ) IGF 1 ( 120 mg / kg / day , s . c . ; n = 5 ) or IGF 1 ( 120 mg / kg / day , s . c . ; n = 5 ) or IGF 1 s . c . ; n = 4 ) . ^^^ Serum IGFBP 3 was consistently elevated by IGF 1 infusion , whereas levels in analog treated monkeys were similar to those in control females . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This was due to a decrease of IGF 1 , associated with a trend to an increase in IGF 1 binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Plasma Insulin like growth hormone 1 ( IGF 1 ) and IGF binding protein 3 ( IGF BP 3 ) concentrations were measured using commercial RIA kits . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At 18 weeks postlesion , it was found that the serum levels of GH , IGF 1 , total IGFBP , and IGFBP 3 of LH rats maintaining reduced body protein were not different from those of age matched controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Substitutions for hydrophobic amino acids in the N terminal domains of IGFBP 3 and 5 markedly reduce IGF 1 binding and alter their biologic actions . ^^^ Insulin like growth factor binding protein 3 and 5 ( IGFBP 3 and 5 ) have been shown to bind insulin like growth factor 1 and 2 ( IGF 1 and 2 ) with high affinity . ^^^ These studies were undertaken to determine if mutagenesis of several of these residues resulted in a reduction of the affinity of IGFBP 3 and 5 for IGF 1 . ^^^ The physiologic consequence of this reduction was that IGFBP 3 and 5 became very weak inhibitors of IGF 1 stimulated cell migration and DNA synthesis . ^^^ The availability of these mutants will make it possible to determine if there are direct , non IGF 1 dependent effects of IGFBP 3 and 5 on cellular physiologic processes in cell types that secrete IGF I . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured basal levels of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 together with 24 h urinary GH excretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The coadministration of IGF binding protein 3 ( IGFBP 3 , the predominant IGF binding protein in serum ) with IGF 1 limits IGF 1 inducible side effects , but it does not attenuate the ability of IGF 1 to enhance protein synthesis and bone accretion ; therefore , we determined whether IGF I / IGFBP 3 would retain biological activity in type 1 DM and limit side effects associated with free IGF 1 administration . ^^^ Twelve patients received recombinant human IGF 1 plus IGFBP 3 ( 2 mg / kg day ) by continuous sc infusion for 2 weeks . ^^^ IGFBP 2 ( an IGF 1 dependent responsive variable ) rose from 141 + / 56 to 251 + / 98 ng / mL ( P < 0 . 01 ) on IGF I / IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and 24 h urinary GH excretion as a measure of pituitary GH secretion were determined at baseline and on days 5 and 9 . ^^^ Baseline IGF 1 and IGFBP 3 levels and GH excretion were significantly diminished compared to those in age matched controls . ^^^ There was a dose dependent increase in IGF 1 and IGFBP 3 during GH treatment . ^^^ In conclusion , GH secretion and serum levels of IGF 1 and IGFBP 3 are diminished in patients with moderate ischemic heart failure . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) replacement during growth hormone receptor antagonism normalizes serum IGF binding protein 3 and markers of bone formation in ovariectomized rhesus monkeys . ^^^ Previous work from this laboratory has shown that the constant sc infusion of insulin like growth factor 1 ( IGF 1 ) to normal pituitary monkeys results in a sustained elevation in circulating concentrations of IGF binding protein 3 ( IGFBP 3 ) , whereas the acute administration of IGF 1 to monkeys pretreated with a GH receptor antagonist produces a brief , but significant , elevation in serum IGFBP 3 . ^^^ The present study tested the hypothesis that the constant infusion of IGF 1 would normalize serum concentrations of IGFBP 3 in females treated with the GH receptor antagonist . ^^^ Within 48 h of the initiation of treatment with the GH receptor antagonist , serum IGF 1 and IGFBP 3 were decreased by 40 % and 18 % from baseline , respectively , and levels continued to decline through the remainder of treatment . ^^^ However , within 48 h of the initiation of IGF 1 administration during GH receptor antagonist treatment , both serum IGF 1 and IGFBP 3 were elevated and normalized to baseline values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 increased the abundance of both intact IGFBP 3 and 5 in the culture medium . ^^^ Testosterone increased IGFBP 3 , and the combination of IGF 1 and testosterone had an additive effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The purpose of this study was to determine the effects of aging and caloric restriction ( CR ) on insulin like growth factor 1 ( IGF 1 ) , IGF 1 receptor ( IGF IR ) , IGF binding protein 3 ( IGFBP 3 ) and IGFBP 4 expression in the stomach and colon of male Fischer 344 rats . ^^^ Effects of aging and caloric restriction on IGF 1 , IGF 1 receptor , IGFBP 3 and IGFBP 4 gene expression in the rat stomach and colon . ^^^ Stomach IGF 1 , IGFBP 3 and IGFBP 4 mRNA levels increased significantly ( P < / =0 . 05 ) , while colonic IGF 1 mRNA levels were unchanged in aged AL rats . ^^^ Increased expression of stomach IGF 1 , IGFBP 3 and IGFBP 4 in aged AL rats suggests that the stomach attempts to preserve IGF activity by increasing local expression of IGF 1 and IGFBPs . ^^^ Because the aging colon has a propensity to develop cancer , it may adapt to increased colonic IGF 1 expression by reducing IGF IR and IGFBP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Studying deficient growth in this hypoplastic infant ( birth weight 1405 g , birth length 36 cm ) with dysmorphic features , the following changes in IGFs and IGFBPs were detected ( microg / l ) : IGF 1 : 26 . 5 vs 48 . 1 in healthy infants ; IGF 2 : 420 vs 728 ; IGFBP 2 : 931 vs 524 ; IGFBP 3 : 800 vs 1070 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULT ( s ) : The IGF 1 levels in peritoneal fluid were significantly higher in patients with endometriosis than in control patients , while the IGFBP 3 levels and the relative proportion of IGFBP 2 in peritoneal fluid were significantly lower in patients with endometriosis than in control patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This assertion is supported by studies of ovariectomized heifers , in which increased stromal IGFBP 3 and reduced IGF 1 correspond with a failure of udder development . ^^^ In vitro studies convincingly demonstrate that much of the mitogenic activity of mammary extracts or serum can be attributed to IGF 1 and that alterations in IGFBP 3 modulate its effectiveness . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in plasma were measured by use of immunoassay kits . ^^^ RESULTS : Although not statistically significant , the mean levels of IGF 1 and the molar ratio of IGF I / IGFBP 3 were higher in patients with advanced or poorly differentiated disease than in patients with early or well differentiated disease . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In conclusion , the effect of stimulated endogenous GH with KP 102 administration increased plasma IGF 1 , 38 43 kDa IGFBP 3 and 24 kDa IGFBP 4 levels in the HI group of growing Holstein steers , but not in the LI one . ^^^ Thus , we strongly believe that the plasma IGF 1 and IGFBPs response to KP 102 treatment is modulated by the nutritional status of growing Holstein steers and the increased plasma IGF 1 concentration with KP 102 treatment may be regulated by plasma 38 43 kDa IGFBP 3 and 24 kDa IGFBP 4 in Holstein steers . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) is an important mitogen , and IGF binding protein 3 ( IGFBP 3 ) has opposing effects . ^^^ We examined whether prediagnostic plasma levels of IGF 1 and IGFBP 3 influence risk of colorectal cancer and adenoma in women . ^^^ After matching controls ( 2 : 1 for cancers and 1 : 1 for adenomas ) to cases by age , month of blood draw , fasting status , and indication for endoscopy ( for adenoma controls ) , plasma IGF 1 and IGFBP 3 levels were measured . ^^^ Controlling for IGFBP 3 level , relative to women in the low tertile of IGF 1 , those in the high tertile were at elevated risk of intermediate / late stage colorectal neoplasia adenoma [ multivariate relative risk ( RR ) , 2 . 78 ; 95 % confidence interval ( CI ) , 0 . 76 9 . 76 ] and cancer ( RR , 2 . 18 ; 95 % CI , 0 . 94 5 . 08 ) . ^^^ Controlling for IGF 1 level , relative to women in the low tertile of IGFBP 3 , women in the high tertile of IGFBP 3 were at lower risk of intermediate / late stage colorectal adenoma ( RR , 0 . 28 ; 95 % CI , 0 . 09 0 . 85 ) and cancer ( RR , 0 . 28 ; 95 % CI , 0 . 10 0 . 83 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
R 3 IGF 1 , an agonist unaffected by IGFBPs , elicited concentration dependent increase in growth , measured by [ ( 3 ) H ] thymidine incorporation , and production of IGFBP 3 , IGFBP 4 , and IGFBP 5 , measured by Western blot . ^^^ Antagonists of the IGF 1 receptor , IGF 1 Analog or monoclonal antibody 1H7 , elicited concentration dependent inhibition of growth and decrease in IGFBP 3 , IGFBP 4 , and IGFBP 5 production , implying that endogenous IGF 1 stimulated growth and IGFBP production . ^^^ R 3 IGF 1 induced increase in IGFBP 3 , IGFBP 4 , and IGFBP 5 production was partially inhibited by a mitogen activated protein ( MAP ) kinase or a phosphatidylinositol 3 kinase ( PI 3 kinase ) inhibitor and abolished by the combination . ^^^ We conclude that endogenous IGF 1 stimulates growth and IGFBP 3 , IGFBP 4 , and IGFBP 5 production in human intestinal smooth muscle cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : The authors measured insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in the CSF of patients with INCL by radioimmunoassay at an early stage when myelin was starting to diminish . ^^^ RESULTS : The authors found low CSF IGF 1 but normal IGFBP 3 in patients with INCL compared with control subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 levels were lower and those of IGFBP 1 higher in nonsuppressor than in suppressor cases and in healthy controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of octreotide , growth hormone ( GH ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and insulin like growth factor 1 ( IGF 1 ) as well as clinical safety were evaluated over a period of 112 days ( 16 weeks ) . ^^^ The suppression of IGF 1 was statistically significant compared to the baseline ( p < 0 . 05 ) through day 63 ; however , there were no appreciable changes in GH and IGFBP 3 concentrations after a single injection of microencapsulated octreotide acetate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The aim of this study was to evaluate the effect of insulin like growth factor 1 ( rhIGF 1 ) in complex with binding protein 3 ( IGFBP 3 ) compared to the effect of free IGF 1 on muscle protein biosynthesis in undernourished animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This investigation examines the effects of orally induced alkalosis on serum IGF 1 and IGFBP 3 concentrations in response to an acute 90 s bout of high intensity cycle exercise . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In saline treated rats , reinnervation was only partial and there was a marked muscle fibre atrophy , whereas , glycosaminoglycan treatment of lesioned rats increased IGF 1 mRNA and protein in the reinnervated muscle , and IGF 1 and insulin like growth factor binding protein 3 plasma levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These results suggest that Qi training influences the growth hormone , IGF 1 and IGFBP 3 status via brain target organ axis in men . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the rabbit , IGF 1 binds to the embryonic coats from Day 3 of development onward by a 38 kDa protein that is probably insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ In the present study , ligand , Western , and Northern blot analyses were used to demonstrate the presence of IGF 1 binding activity , several immunoreactive IGFBP 3 proteins , and IGFBP 3 mRNA in horse conceptuses with particularly large amounts of immunoreactive IGFBP 3 in the conceptus capsule . ^^^ Thus , most of the IGFBP 3 contained within the capsule , which binds IGF 1 to this special extracellular matrix of the preimplantation horse conceptus , is likely to be embryonic in origin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We compared the effects of a moderate intensity seated resistance training program with a high intensity standing free weight exercise program on bone mass and serum levels of IGF 1 and IGFBP 3 in healthy older men and women . ^^^ Neither circulating serum IGF 1 nor IGFBP 3 were altered by either training regimen , but both training programs resulted in improvements in total body strength ( 37 . 62 % ) and lean mass ( males 4 . 1 % , females 3 . 1 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Clinical and hormonal characterization included physical examination and measurement of GH , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , cortisol , prolactin , LH , FSH , and free thyroxine ( FT 4 ) . ^^^ Serum GH , IGF 1 , IGF 1 SDS , IGFBP 3 and IGFBP 3 SDS were all significantly lower in the mt / mt group than in the wt / mt and wt / wt groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This modest phenotype was observed despite reductions of 62 and 88 % in the concentrations of plasma IGF 1 and IGFBP 3 , respectively . ^^^ Therefore , ALS is required for postnatal accumulation of IGF 1 and IGFBP 3 but , consistent with findings supporting a predominant role for locally produced IGF 1 , is not critical for growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : We evaluated the relations of plasma concentrations of IGF 1 and IGFBP 3 with vertex balding in middle aged and elderly men . ^^^ Odds ratios ( ORs ) of vertex balding associated with IGF 1 and IGFBP 3 were estimated from logistic regression models mutually adjusting for each other and controlling for age at blood draw . ^^^ CONCLUSION : Older men with vertex balding have lower circulating levels of IGFBP 3 and higher levels of IGF 1 when controlling for IGFBP 3 level . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , IGFBP 3 inhibits the acceleration of dissociation rates due to increased IGF 1 levels . ^^^ Thus , under receptor saturating conditions IGFBP 3 may act to increase mitogenesis by increasing the residence time of individual molecules of IGF 1 upon the type 1 IGF receptor . . ^^^ Free ligand accelerated dissociation of insulin like growth factor 1 ( IGF 1 ) from the type 1 IGF receptor is reduced by insulin like growth factor binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 concentrations were determined by radioimmunoassays and acid labile subunit ( ALS ) was measured by ELISA . ^^^ A shift in distribution of IGFBP 3 and IGF 1 in RA and OA synovial fluids ( RASynF , OASynF ) and an associated increase in ALS suggested the presence of 150 kDa ternary complexes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Transforming growth factor beta 1 inhibited IGFBP 3 ( P < 0 . 01 ) , IGFBP 4 ( P < 0 . 01 ) , and IGF 1 mRNA expression , whereas at the protein level only IGFBP 3 was significantly decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The prevalence of the latter is increasing in Western populations , and a combination of high IGF 1 and low IGF binding protein 3 concentrations has been associated with the presence of DCIS lesions in premenopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There were no significant differences between the concentrations of GH , IGF 1 , IGF bioactivity and IGFBP 3 protease activity before and after dexamethasone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : Measurements were patient demographics , incidence of sepsis , inhalation injury , mortality , serum constitutive proteins , acute phase proteins , proinflammatory cytokines and insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP ) 1 , and IGFBP 3 . ^^^ Serum IGF 1 and IGFBP 3 increased with rHGH treatment , whereas serum IGFBP 1 decreased compared with placebo ( p < . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Resumption of GH after placebo was followed by increments in serum IGF 1 ( microg / L ) [ from 147 . 8 + / 33 . 4 to 452 + / 76 ( P = 0 . 001 ) ] and IGF binding protein 3 , as well as in fasting glucose ( mmol / L ) [ 4 . 9 + / 0 . 2 vs . 5 . 3 + / 0 . 2 ( P = 0 . 03 ) ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In basal samples , we evaluated the circulating levels of IGF 1 , IGF BP 3 and their ratio , SHBG , FSH , LH , Oestradiol ( E 2 ) and PRL . ^^^ Insulin like growth factor type 1 ( IGF 1 ) , Insulin like growth factor binding protein 3 ( IGF BP 3 ) , FSH , LH and PRL were measured by Radioimmunoassay ( RIA ) . ^^^ No significant variations were found in IGF BP 3 levels or in the IGF 1 / IGF BP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We assessed IGF 1 and IGFBP 3 expression , critical mediators in different models of pulmonary fibrosis , in nine Tx patients . ^^^ Our data strongly argue for a role of IGF 1 and IGFBP 3 in the fibrotic process underlying BOS , and for their possible value as an early marker of this complication . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As correlative studies on IGF 1 , IGF BP 3 and bone mass in the elderly are lacking , we studied the relationships between serum IGF 1 , IGF BP 3 , bone mineral density ( BMD ) , body mass index ( BMI ) , calciotropic hormones and age in 102 premenopausal and postmenopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Differential effects of IGF binding proteins , IGFBP 3 and IGFBP 5 , on IGF 1 action and binding to cell membranes of immortalized human chondrocytes . ^^^ The stimulatory effect of IGF 1 was inhibited significantly by addition of IGFBP 3 but enhanced slightly by IGFBP 5 . ^^^ Binding of ( 125 ) 1 labeled IGF 1 to the cells was displaced by both IGFBP 3 and IGFBP 5 , although higher concentrations of unlabeled IGFBP 5 were required to displace IGF 1 to the same extent as IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Vitreous levels of IGF 1 , IGF binding protein 1 , and IGF binding protein 3 in proliferative diabetic retinopathy : a case control study . ^^^ OBJECTIVE : To evaluate vitreous levels of IGF 1 and its binding proteins IGFBP 1 and IGFBP 3 in patients with proliferative diabetic retinopathy ( PDR ) . ^^^ Both groups were matched by age , serum IGF 1 , IGFBP 1 , and IGFBP 3 levels . ^^^ Serum and vitreous levels of IGF 1 , IGFBP 1 , and IGFBP 3 were measured by immunological methods . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , when we classified the pGHD children into 2 groups according to IGFBP 3 SDS for normal Thai children , we found that the mean of free IGF 1 levels in pGHD children with IGFBP 3 SDS < or = 1 . 3 was significantly lower than that of the controls . ( 0 . 68 + / 0 . 55 vs 2 . 66 + / 0 . 71 ng / ml , p = 0 . 04 ) In conclusion , the measurement of free IGF 1 level can be used to evaluate the GH status of short children and might be used as a guide when starting treatment . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our previous studies have indicated that insulin like growth factor 1 ( rhIGF 1 ) in complex with its binding protein 3 ( IGFBP 3 ) , but not free IGF 1 , was a potent stimulator of muscle protein synthesis in rats with chronic undernutrition . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PV was also correlated with GH ( r= 0 . 52 , P=0 . 0026 ) , IGF 1 ( r= 0 . 62 , P=0 . 0002 ) and IGF binding protein 3 ( IGFBP 3 ) levels ( r= 0 . 39 , P=0 . 032 ) but neither with testosterone or dihydrotestosterone ( DHT ) levels . ^^^ In GHD patients TZV but not PV was correlated with age ( r=0 . 58 , P=0 . 0007 ) and neither TZV nor PV were correlated with GH , IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Systemic levels contribute significantly to increased intraocular IGF 1 , IGF 2 and IGF BP 3 [ correction of IFG BP 3 ] in proliferative diabetic retinopathy . ^^^ This was comparable to an increase of IGF I / 11 and IGF BP 3 in vitreous from PDR patients ( IGF 1 : 2 . 3 + / 1 . 1 ng / ml p = 0 . 005 . ^^^ IGF BP 3 : 97 . 9 + / 26 . 9 ng / ml ; p = 0 . 0001 ; n = 34 ) compared to controls ( IGF 1 : 0 . 7 + / 0 . 1 ng / ml . ^^^ Serum levels did not differ significantly among the groups regarding IGF 1 , 2 and IGF BP 3 . ^^^ This study demonstrates that influx of IGF 1 , 2 and IGF BP 3 in PDR quantitatively parallels influx of the liver derived serum protein albumin suggesting that leakage of the blood retina barrier and serum levels of IGF primarily determine intravitreal IGF levels rather than local synthesis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 was originally defined by the somatomedin hypothesis as the principal carrier of IGF 1 in the circulation and the primary regulator of the amount of free IGF 1 available to interact with the IGF 1 receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , and its binding proteins ( IGFBP 3 , IGFBP 4 , and IGFBP 5 ) , as well as bone mineral density ( BMD ) at the lumbar spine , femoral neck , and radius midshaft were measured before and 1 year after antithyroid ( methimazole ) treatment . ^^^ Serum free thyroxine showed a positive correlation with serum levels of IGF 1 ( r = 0 . 73 , p < 0 . 05 ) , IGFBP 3 ( r = 0 . 59 , p < 0 . 05 ) , and IGFBP 4 ( r = 0 . 67 , p < 0 . 05 ) but not IGFBP 5 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH activity and GH status were determined by measuring serum levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were obtained for the measurement of BG at 0200 , 0400 and 0700 , and of insulin , total IGF 1 ( tIGF 1 ) , fIGF 1 , IGFBP 1 and IGFBP 3 at 0700 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our study shows that , with aging , colonic expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) is significantly increased and decreased , respectively . ^^^ No significant change in proliferation of colonic crypt cells is observed by bromodeoxyuridine ( BrdU ) labeling , although the increased colonic expression of IGF 1 and the decreased expression of IGFBP 3 with aging may result in an increased colonic IGF 1 bioactivity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This growth depression is associated with large decreases in the plasma concentrations of both IGF 1 and IGFBP 3 , indicating the critical role of ALS in the regulation of circulating levels of these proteins . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH treated CRF children show catch up growth that correlates positively with a rise in each component of the 150 kDa serum ternary complex ( IGF 1 or II / IGFBP 3 or 5 / acid labile subunit ) ; IGFBP 1 , 2 and 6 levels do not rise , but serum IGF bioactivity does . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the effect of fenretinide on circulating IGF 1 , IGF 2 and IGFBP 3 measured at yearly intervals during the 2 year treatment period and one year after treatment discontinuation in a predominantly male population of patients with superficial bladder cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a cross sectional analysis within the Nurses ' Health Study , we compared the associations of plasma levels of endogenous IGF 1 and IGFBP 3 with breast density in 65 premenopausal and 192 postmenopausal women . ^^^ Generalized linear models and Spearman ' s partial correlation coefficients described the associations between breast density and IGF 1 , IGFBP 3 , and the IGF 1 : IGFBP 3 ratio . ^^^ Premenopausal breast density was positively correlated with IGF 1 and inversely correlated with IGFBP 3 ; the association was strongest for the IGF 1 : IGFBP 3 ratio and breast density ( r = 0 . 39 ; P = 0 . 004 ) . ^^^ In contrast , the correlation between breast density and the IGF 1 : IGFBP 3 ratio among postmenopausal women was 0 . 02 ( P = 0 . 83 ) . ^^^ The associations of IGF 1 : IGFBP 3 ratio with breast density differed significantly between premenopausal and postmenopausal women ( P = 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : Randomized , double blind trial of three different nutritional supports and analysis of serum IGF 1 , IGFBP 3 , and serum IGFBP 3 proteolysis . ^^^ MEASUREMENTS AND MAIN RESULTS : Serum IGF 1 and IGFBP 3 were measured by radioimmunoassay every 3 days for 28 days in 23 severely burned adults . ^^^ Serum IGFBP 3 concentration from ligand binding assay was correlated with serum proteolytic capacity in all subjects ( mean r 2 = 0 . 77 ; p < . 01 ) and with serum IGF 1 concentrations in five of six subjects ( mean r 2 = 0 . 81 ; p < . 01 ) . ^^^ The proteolysis of IGFBP 3 may be an important cause of the decreased serum IGF 1 values and the protease ( s ) responsible for this seem to be similar to those observed in late pregnancy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum GH and levels of serum insulin like growth factor 1 ( s IGF 1 ) and serum insulin like growth factor binding protein 3 ( s IGFBP 3 ) were measured . ^^^ Median IGF 1 SDS and IGFBP 3 SDS were 2 . 5 ( 5 . 2 0 . 7 SDS ) and 1 . 7 ( 5 . 8 0 . 9 SDS ) , respectively , and IGF 1 SDS was correlated to peak GH ( rs = 0 . 45 , P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to determine the effect of aging on serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) levels following myocardial infarction ( MI ) . ^^^ For four consecutive days , we monitored the variation of serum IGF 1 , IGF 2 and IGFBP 3 concentrations in 26 patients aged 19 71 years who were diagnosed with MI . ^^^ Serum IGF 1 , IGF 2 and IGFBP 3 were measured daily by double antibody radioimmunoassay . ^^^ MI patients in both groups showed significantly lower IGF 1 and IGF 2 ( IGFs ) with higher IGFBP 3 compared to age and sex adjusted levels of normal adults ( controls ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The usefulness of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) for evaluation of children with short stature . ^^^ The diagnostic value of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) was studied in 24 growth hormone deficient ( GHD ) and 36 normal variant short stature ( NVSS ) children . ^^^ The serum IGF 1 and IGFBP 3 concentrations were markedly below the 5th centile for chronological age in all 24 GHD children , but were in the low normal range for age in most of the NVSS children . ^^^ The concentrations of IGF 1 and IGFBP 3 significantly correlated with peak GH concentration , height age , and bone age . ^^^ To account for the age and sex dependency , IGF 1 and IGFBP 3 levels were transformed to standard deviation score ( SDS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Taken together , these observations confirm that IGFBP 3 fibronectin interactions affect the IGF 1 axis , and they indicate that IGF 1 is stored in the chondrocyte territorial matrix through binding to a complex of IGFBP 3 and intact fibronectin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Small but significant increases were found in both the resting and exercising arm for IGF 1 and IGF binding protein 3 ( IGFBP 3 ) . ^^^ Unilateral wrist exercise was not sufficiently intense to increase circulating lactate or heart rate , but it led to systemic changes in GH , IGF 1 , IGFBP 3 , and FGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High serum levels of insulin like growth factor 1 ( IGF 1 ) and low levels of IGF binding protein 3 ( IGFBP 3 ) have been shown to correlate with increased prostate cancer risk . ^^^ To evaluate this , IGF 1 , IGFBP 3 , and prostate specific antigen ( PSA ) were measured in serum from 665 consecutive men ( 179 with prostate cancer ) , aged 55 67 yr , with elevated serum prostate specific antigen ( PSA ; > or = 4 microg / L ) in a screening trial . ^^^ Men in the highest quartile of IGF 1 levels had an odds ratio ( OR ) for prostate cancer of 0 . 50 [ 95 % confidence interval ( CI ) 0 . 26 0 . 97 ] when adjusting for serum IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : We compared graft function , linear growth , immunosuppression and serum IGF 1 ( RIA ) and IGFBP 3 ( IRMA ) concentrations in 15 liver ( 5 . 6+ / 1 . 1 years old ) and 17 renal ( 7 . 4+ / 0 . 1 years old ) Tx patients who were followed for 4 6 years . ^^^ At 3 . 7+ / 0 . 4 years post Tx , liver Tx patients presented higher mean serum IGF 1 level , lower mean serum IGFBP 3 value , leading to a higher mean IGF I / IGFBP3 molar ratio , P < 0 . 001 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MATERIALS AND METHODS : We have investigated the effect of SA on prostate cancer cell ( DU 145 , LNCaP and PC 3 ) growth and expression of IGFs ( IGF 1 , IGF 2 and IGF 1 receptor ( IGF IR ) ) , IGFBP 3 and vascular endothelial growth factor ( VEGF ) . ^^^ Gene expression studies by RT PCR have demonstrated a decrease in mRNAs of IGF 2 in DU 145 , IGF 1 , and IGF IR in LNCaP , and IGF 2 and VEGF in PC 3 cells and an increase in IGFBP 3 in both DU 145 and PC 3 cells by treatment with SA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The mitogenic effect of IGF 1 could be inhibited by both IGFBP 2 and IGFBP 3 showing that these binding proteins modulate the bioactivity of IGF 1 in the mammary gland at the cellular level . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , 12 pairs of significantly coexpressed IGF system components were detected ( IGF 1 < > IGF 1R , IGF 1 < > IGF 2R , IGF 1 < > IGFBP 3 , IGF 1 < > IGFBP 6 , IGFBP 3 < > IGF 1R , IGFBP 6 < > IGF 1R , IGFBP 1 < > IGF 2R , IGFBP 3 < > IGF 2R , IGFBP 5 < > IGF 2R , IGFBP 3 < > IGFBP 5 , IGFBP 3 < > IGFBP 6 , IGFBP 5 < > IGFBP 6 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Preincubation of cultured bovine fibroblasts with 50 nM IGFBP 3 had no effect alone , but enhanced by 3 to 4 fold IGF 1 stimulated 3H aminoisobutryric acid ( AIB ) uptake . ^^^ However , the sensitivity of PKB / Akt to activation by IGF 1 was increased by 2 to 4 fold with IGFBP 3 pretreatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have examined the regulation of an insulin like growth factor binding protein 3 ( IGFBP 3 ) protease secreted by MCF 7 human breast cancer cells using a ligand binding assay that relies on the decrease in affinity for des ( 1 3 ) IGF 1 that occurs when IGFBP 3 becomes proteolyzed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To obtain some initial information about potential endocrine factors , we measured the serum concentrations of GH , IGF 1 , IGFBP 3 and GHBP in healthy young men shorter than 159 cm and taller than 187 cm . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum GH , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and insulin levels were measured in patients . ^^^ No correlations were found between lipoprotein ( a ) and IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Monitoring serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , IGF I / IGFBP 3 molar ratio and leptin during growth hormone treatment for disordered growth . ^^^ The aim of this study was to evaluate changes in ( 1 ) serum IGF 1 , IGFBP 3 and IGF I / IGFBP 3 molar ratio , and ( 2 ) serum leptin , an indirect marker of GH response , during the first year of GH treatment in children with disordered growth . ^^^ MEASUREMENTS : Serum IGF 1 , IGFBP 3 and leptin levels were measured before treatment and after 6 weeks , 3 months , 6 months and 12 months of GH treatment , with a mean dose of 0 . 5 IU / kg / wk in GHD and 0 . 7 IU / kg / wk in non GHD groups . ^^^ IGF 1 , IGFBP 3 and the calculated IGF I / IGFBP 3 molar ratio were expressed as SD scores using reference values from the local population . ^^^ IGF 1 ( 1 . 8 SDS + / 2 . 2 ) and IGFBP 3 ( 1 . 1 SDS + / 0 . 6 ) levels and their molar ratios were highest at 6 weeks and remained relatively constant thereafter . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fetal GH infusion raised the circulating fetal concentrations of GH threefold , but did not change fetal concentrations of IGF 1 , IGF binding protein 3 , insulin or ovine placental lactogen . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A prospective study of plasma levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 , and colorectal cancer risk among men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 and the risk of colorectal adenoma and cancer in the Nurses ' Health Study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study evaluated the regulation of IGFBPs in human aorta smooth muscle cells by cyclic AMP , dexamethasone and IGF 1 . cAMP decreased IGFBP 3 , increased IGFBP 4 and increased IGFBP 6 . ^^^ IGF 1 increased IGFBP 3 and IGFBP 6 while decreasing IGFBP 4 . ^^^ Co incubation with IGF 1 and dexamethasone or cAMP increased media IGFBP 3 , despite a decrease in IGFBP 3 mRNA , due to the dominant effect of IGF 1 induced dissociation of cell surface bound IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Systemic treatment with epidermal growth factor causes organ growth concomitant with reduced circulating levels of IGF 1 and IGFBP 3 : time dependent changes in female rats . ^^^ Circulating GH was unchanged but IGF 1 and IGFBP 3 were reduced approximately 25 and 45 % , respectively , in all EGF treated groups . ^^^ In conclusion , systemic EGF treatment causes visceral growth concomitant with reduced circulating levels of IGF 1 and IGFBP 3 in mature female rats . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The production by the liver of the three subunits of the growth hormone ( GH ) dependent 150 kDa complex ( IGF 1 , IGF binding protein 3 and acid labile subunit or ALS ) is primarily under the control of GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To examine the hypothesis that anthropometric measures and physical fitness influence circulating insulin like growth factor 1 ( IGF 1 ) during puberty , we measured IGF 1 , free IGF 1 , IGFBP 1 , and IGFBP 3 concentrations in 156 healthy girls ( 9 16 years old ) characterized by aerobic capacity ( VO2max ) , fat free mass ( FFM ) , percent fat mass and pubertal development . ^^^ IGF 1 , free IGF 1 and IGFBP 3 increased with pubertal development while IGFBP 1 declined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have compared the effects of treating lactating ewes for 5 days with an equimolar dose ( 0 . 1 mg / kg / day , administered as two equal doses 12 hourly ) of either bovine growth hormone ( bGH ) ( n = 10 ) , oPL ( n = 10 ) or saline ( n = 9 ) on hepatic and mammary GHR , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) gene expression and hepatic GHR number . ^^^ There was no effect of either bGH or oPL treatment on mammary GHR , IGF 1 or IGFBP 3 mRNA or hepatic GHR number . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) were low on admission , and in the 8 patients whose serum IGF 1 levels failed to increase over 30 days , levels of the other proteins also remained low , while IGFBP 3 proteolytic activity increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with pair fed rats , zinc deficiency produced attenuated weight gain ( 43 % , P < 0 . 001 ) , lower serum IGF 1 and liver IGF 1 mRNA ( 52 % , P < 0 . 001 and 44 % , P < 0 . 05 ) , lower serum IGFBPs ( IGFBP 3 66 % , IGFBP 4 48 % , 34 29 kDa IGFBP cluster 53 % , P < 0 . 05 ) , lower liver GHR and its mRNA ( 20 and 34 % , P < 0 . 05 ) and lower serum growth hormone binding protein ( GHBP ) and its mRNA ( 56 and 48 % , P < 0 . 05 ; all comparisons vs PF rats ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the protein levels for IGF 1 receptor and IGFBP 3 and 4 did not correlate with mRNA levels . ^^^ There were no differences in mRNA levels for IGF 1 receptor and IGFBP 3 and 4 between diabetic and non diabetic HREC cultures , suggesting a post transcriptional regulation of IGF 1 receptor and the two IGFBPs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 receptors on blood cells : their relationship to circulating total and `` free ' ' IGF 1 , IGFBP 1 , IGFBP 3 and insulin levels in healthy subjects . ^^^ IGFBP 3 levels were inversely related to the number of IGF 1 binding sites on erythrocytes and to the K ( d ) of the IGF 1 receptor on PBMCs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The assays of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) per se do not establish the diagnosis of adult GHD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The endpoints evaluated included plasma or serum concentrations of bone specific alkaline phosphatase , osteocalcin , insulin like growth factor 1 ( IGFI ) , IGF binding protein 3 ( IGFBP 3 ) , and urine concentrations of deoxypyridinoline cross links and carboxy terminal telopeptide of type 1 collagen . ^^^ In premenopausal women , IGFI and IGFBP 3 concentrations were increased by the low iso diet , and deoxypyridinoline cross links was increased by both the low and high iso diets during certain phases of the menstrual cycle . ^^^ In postmenopausal women , bone specific alkaline phosphatase was decreased by both the low and high iso diets , and there were trends toward decreased osteocalcin , IGFI , and IGFBP 3 concentrations with increasing isoflavone consumption . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant relationships were found between absolute levels or changes in nu LH concentrations and leptin , IGF 1 , IGFBP 3 , or IGFBP 1 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) may be risk factors for the development of colorectal cancer . ^^^ These contrasting backgrounds led us to investigate the relationship between serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and the presence of colorectal adenomas , known precursors of colorectal carcinoma , in 345 volunteers attending a screening flexible sigmoidoscopy trial ( entry criteria : healthy , aged 55 64 yr ) . ^^^ However , there was no association found between either serum IGF 1 and / or IGFBP 3 and the presence of adenomas . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The following variables were investigated : age , gender , duration of GHD , body composition , serum levels of high affinity GHBP , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Localization of expression of IGF 1 , IGFBP 3 , and IGFBP 5 mRNAs in jejunum was accomplished by in situ hybridization . ^^^ IGF 1 or GH stimulated IGFBP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment of confluent C 6 cells with 200 microg / ml Poly ( IC ) for 24 h decreased levels of immunoreactive IGF 1 in conditioned medium ( CM ) to 55 % of the control value , decreased IGF 1 receptor beta subunit levels to 28 % of the control value , and decreased levels of IGFBP 3 , IGFBP 4 , and IGFBP 5 protein in CM to 45 % , 50 % , and 30 % of the control values , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Colorectal cancer risk showed a modest but statistically nonsignificant positive association with levels of IGF 1 and was statistically significantly increased for the highest quintile of IGFBP 3 ( OR = 2 . 46 [ 95 % CI = 1 . 09 5 . 57 ] ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Normal IGF 1 and enhanced IGFBP 3 response to very low rhGH dose in patients with dilated cardiomyopathy . ^^^ In CS 4 day rhGH increased IGF 1 levels ( 222 . 4+ / 14 . 9 microg / l ; p < 0 . 01 vs baseline ) but did not modify IGFBP 3 levels ( 3 . 0+ / 0 . 2 mg / l ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) as serum markers of prostate cancer ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The combined treatment of IGF 1 and FSH increased the concentration of IGFBP 3 in OCC and MGC conditioned media by 4 and 6 fold , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study explored the relationships of serum insulin like growth factors , IGF 1 and IGF 2 , and their binding proteins ( IGFBP ) 2 and IGFBP 3 , with key clinicopathological parameters in 92 patients with colorectal cancer ( cases ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGFs ) circulate in plasma as part of a 150 kD complex that also contains IGF binding protein 3 ( IGFBP 3 ) , a protein that binds IGF 1 and IGF 2 with high affinity , and an acid labile subunit ( ALS ) that does not directly bind IGFs . ^^^ Significant correlations ( p < 0 . 001 ) were seen between total and free ALS concentrations and IGF 1 ( r = 0 . 50 and 0 . 60 , respectively ) , free IGF 1 ( r = 0 . 37 and 0 . 36 ) , IGF 2 ( r = 0 . 37 and 0 . 27 ) , IGFBP 1 ( r = 0 . 48 and 0 . 49 ) , IGFBP 2 ( r = 0 . 44 and 0 . 51 ) and IGFBP 3 ( r = 0 . 67 and 0 . 59 ) at all Tanner stages . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFI was above the 10th percentile for children with idiopathic short stature in 10 of 15 patients with craniopharyngioma induced GH deficiency and IGF binding protein 3 in 14 of 15 patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The levels of IGF 1 , IGF 2 and IGFBP 3 were determined by specific radioimmunoassays ( RIAs ) . ^^^ Moreover , a statistical and positive correlation between the levels of IGF 1 and IGFBP 3 was noted . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At d 130 of fetal life , fetuses suffering from chronic undernutrition caused by placental insufficiency had lower expression of the ALS and IGF 1 genes than well nourished fetuses , but they did not have any changes in the expression of the IGF binding protein ( IGFBP ) 2 or IGFBP 3 genes . ^^^ The lower plane of nutrition decreased the expression of the ALS , IGF 1 , and GH receptor genes and increased the expression of the IGFBP 2 gene ; expression of the IGFBP 3 gene was not affected by nutrition at this stage of life . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study evaluated the ability of insulin like growth factor 1 ( IGF 1 ) complexed with IGF binding protein 3 ( IGFBP 3 ) to modulate the sepsis induced inhibition of protein synthesis in gastrocnemius . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Human intestinal smooth muscle cells in culture produce insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 4 , and IGFBP 5 , which can modulate the effects of IGF 1 on growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
One such target gene is the insulin like growth factor binding protein 3 ( IGFBP 3 ) , an extracellular protein responsible for the carriage of IGF 1 but which can act independently of IGF 1 , inhibiting cell growth and enhancing apoptosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The following type 1 loci were mapped : BCP to BBU8q32 and OAR4q32 , CLCN 1 to BBU8q34 and OAR4q34 , IGFBP 3 to BBU8q24 and OAR4q27 , KRT to BBU4q21 and OAR 3q21 , IFNG to BBU4q23 and OAR3q23 , IGF 1 to BBU4q31 and OAR3q31 , GNRHR to BBU7q32 and OAR6q32 , MTP to BBU7q21 and OAR6q15 , PDE6B to BBU7q36 and OAR6q36 , BF to BBU2p22 and OAR20q22 , EDN 1 to BBU2p24 and OAR20q24 , GSTA 1 to BBU2p22 and OAR20q22 , OLADRB ( MHC ) to BBU2p22 and OAR20q22 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After 2 yr , GH , IGF 1 , and IGFBP 3 levels were decreased , whereas prostate specific antigen levels did not change . ^^^ When patients were divided according to age , prostate volume decreased after 2 yr only in the 8 controlled patients aged below 50 yr , but not in those controlled and with age above 50 yr despite similar decrease in GH , IGF 1 , and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 were quantified in growing Angus bulls ( n = 56 ) to determine their relationship with postweaning growth and carcass ultrasound measurements . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Insulin like growth factor 1 ( IGF 1 ) and its principal binding protein 3 ( IGFBP 3 ) are central in the mediation of the effect of growth hormone , and the IGF system has been reported to play a role in the pathogenesis of childhood leukemia . ^^^ METHODS : To further evaluate the hypothesis connecting the IGF system to this disease , we have examined whether IGF 1 and IGFBP 3 are associated with the two main endogenous risk factors for childhood leukemia , namely gender and birth weight , since boys and heavier newborns are known to be at higher risk . ^^^ IGF 1 and IGFBP 3 were measured under code in the serum of 118 apparently healthy children aged 0 14 years and the values of each of these components were regressed on age , gender and birth weight . ^^^ RESULTS : In the total data set , IGF 1 was positively associated with birth weight ( p = 0 . 0001 ) , whereas girls had higher levels of IGFBP 3 ( p = 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To gain a better understanding of the growth of athyreotic newborns in the first weeks of life , we evaluated auxological parameters and determined the serum levels of growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) and free IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) in 15 hypothyroid infants ( 10 females ) at a mean age of 25 d of life , immediately before the beginning of L thyroxine therapy , and at 3 and 6 mo of life . ^^^ The blood GH , IGF 1 and IGFBP 3 levels were significantly lower in patients at diagnosis than in controls of the same age ( p < 0 . 01 for all parameters ) , as well as IGFBP 3 studied by Western blotting . ^^^ CONCLUSION : Increased IGFBP 3 proteolytic activity in our patients at diagnosis , favouring IGF 1 bioavailability , could account for normal free IGF 1 levels and in turn for their normal growth pattern during the first weeks of life and before the start of treatment . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a prospective case control study nested within a cohort of New York City women , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and C peptide were measured in frozen serum samples from 172 pre menopausal and 115 post menopausal subjects who were subsequently diagnosed with breast cancer . ^^^ Post menopausal breast cancer was not associated with serum IGF 1 , IGFBP 3 or C peptide levels . ^^^ There was no association between pre menopausal breast cancer and IGFBP 3 , IGF 1 : IGFBP 3 ratio or non fasting levels of C peptide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In order to investigate the cyclic changes of serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 levels in menstrual cycles treated with or without antiestrogens ( clomiphene citrate and tamoxifen ) , we treated 24 young women having irregular menstrual cycles with either clomiphene citrate ( 100 mg / day ) ( n = 12 ) or tamoxifen ( 60 mg / day ) ( n = 12 ) from the 5th to the 9th day of the menstrual cycle . ^^^ Unlike cyclic changes in circulating IGFBP 1 , serum concentrations of IGF 1 and IGFBP 3 remained unchanged throughout the menstrual cycle in all groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin , IGF 1 and IGFBP 2 levels in follicular fluids from large follicles did not differ between females whereas IGFBP 3 levels were lower in L 1 than in H gilts ( P < 0 . 05 ) and intermediate in H 1 gilts . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
INTRODUCTION : We examined the relationships between insulin like growth factor 1 ( IGF 1 ) , its binding protein ( IGFBP 3 ) , body composition , and bone mineral density ( BMD ) in collegiate runners ( N = 13 ) , gymnasts ( N = 10 ) , and noncompetitive women ( N = 10 ) . ^^^ METHODS : Subjects were evaluated by dual energy 10 ray absorptiometry for body composition and BMD of the spine , hip , and whole body , fasting serum levels of IGF 1 and IGFBP 3 , and dietary intake . ^^^ The ratio IGF I / IGFBP 3 was calculated as a marker of IGF 1 bioavailability . ^^^ RESULTS : In ANOVA , IGF 1 and IGF I / IGFBP 3 in athletes with oligomenorrhea and amenorrhea did not differ from eumenorrheic athletes ; thus , values were pooled . ^^^ IGF 1 and IGF I / IGFBP 3 differed between groups with gymnasts having higher IGF 1 values than runners ( 397+ / 58 vs 288+ / 73 ng 10 mL ( 1 ) , P < 0 . 001 ) and higher IGF I / IGFBP 3 than controls and runners ( 0 . 065+ / 0 . 009 vs 0 . 056+ / 0 . 008 vs 0 . 045+ / 0 . 009 , P = 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased levels of GH [ 122 micrograms / l ] , insulin like growth factor ( IGF 1 ) [ 830 micrograms / l ] , insulin like growth factor binding protein 3 ( IGFBP 3 ) [ 8 . 6 mg / l ] and PRL [ 590 micrograms / l ] were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 18 patients with hypothalamo pituitary diseases aged over 60 years and in 18 sex , age and BMI matched healthy subjects , the results of plasma IGF 1 and IGF BP 3 levels and the GH response to GHRH + arginine test ( GHRH + ATT ) were correlated to the results of body composition , serum osteocalcin ( OC ) and urinary cross linked N telopeptides of type 1 collagen ( Ntx ) and the bone mineral density ( BMD ) . ^^^ The GH response to GHRH + ATT and ITT was markedly reduced in patients compared to controls ( 3 . 1 + / 0 . 7 vs . 23 . 2 + / 2 . 3 micrograms / L , P < 0 . 001 and 1 . 1 + / 0 . 3 vs . 6 . 4 + / 0 . 8 micrograms / L , P < 0 . 001 ) , so all patients were classified as GHD , though no significant difference was found in plasma IGF 1 and IGF BP 3 levels between the two groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BMI and body composition were determined and we determined baseline serum IGF 1 , IGFBP 3 , insulin and leptin levels and after the IGF 1 generation test performed after the GH administration of 0 . 1 U / kg / day s . c each evening for 4 days . ^^^ As expected the patient had lower baseline IGF 1 and IGFBP 3 with high insulin and leptin levels . ^^^ After IGF 1 generation test , IGF 1 , IGFBP 3 and insulin levels increases without changes in body composition and leptin levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of immunoreactive total and free IGF 1 , and IGF binding protein ( IGFBP ) 2 and IGFBP 3 were determined before and after correction of hyperthyroidism in eight patients with Grave ' s disease and compared to control patients matched for age , sex and pubertal stage . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our studies have focused in detail on IGF 1 and 2 and the major IGFBPs in the circulation , IGFBP 3 , 1 , and 2 ; GH was also measured in some of these studies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effect of IGFBP 3 and the fragments on IGF 1 receptor ( IGFIR ) signaling pathways was studied by testing IGF 1 induced receptor autophosphorylation in IGFIR overexpressing NIH 3T3 cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 3 , and total and free ALS levels were significantly decreased at diagnosis . ^^^ Normalization of IGF 2 and IGFBP 3 occurred 6 months after diagnosis , and normalization of IGF 1 and total and free ALS occurred 1 yr after terminating therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH treatment normalized serum IGF 1 in a majority of the patients and increased IGFBP 3 and 5 as well as IGFBP 4 and IGF 2 to values within normal range . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , we assessed serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) levels and IGFBP 3 circulating forms . ^^^ Overall , IDDM children showed reduced levels of IGF 1 ( mean + / SD , 0 . 65+ / 1 . 9 z score ) and normal levels of IGF 2 ( mean + / SD , 0 . 05+ / 1 . 2 z score ) and IGFBP 3 ( mean + / SD , 0 . 06+ / 1 . 2 z score ) . ^^^ However , whereas patients younger than 6 yr showed normal values of IGF 1 , IGF 2 , and IGFBP 3 , these peptides were significantly reduced in older subjects compared with either younger IDDM children or controls ( P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that 1 ) males are significantly more responsive than females to exogenous GH ; 2 ) the increase in IGF 1 is more robust compared with those in IGFBP 3 and ALS ; 3 ) IGFBP 2 changes very little during GH treatment ; and 4 ) among IGF related substances , IGF 1 is the most specific marker of supraphysiological GH exposure . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 , and prostate specific antigen as predictors of clinical prostate cancer . ^^^ In a prospective , cohort , case control study in the Baltimore Longitudinal Study on Aging population , we examined prostate volume by magnetic resonance imaging , and prostate specific antigen ( PSA ) , IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in sera obtained approximately 9 yr before diagnosis of prostate cancer in cases ( n = 72 ) or age matched controls ( n = 127 ) and in 76 additional Baltimore Longitudinal Study on Aging men ( normal subjects ) with measured prostate volumes and no prostate cancer . ^^^ Adjusted ORs for the high vs . low tertile were : for IGF 1 , 3 . 1 [ confidence interval ( CI ) , 1 . 1 8 . 7 ] ; for IGF 2 , 0 . 2 ( CI , 0 . 07 0 . 6 ) ; for IGFBP 3 , 0 . 71 ( CI , 0 . 3 1 . 7 ) ; and for PSA , 12 . 5 ( CI , 3 . 8 40 . 9 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Size at birth and cord blood levels of insulin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and the soluble IGF II / mannose 6 phosphate receptor in term human infants . ^^^ We measured cord blood levels of IGF 2 , soluble IGF2R , insulin , IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 and examined their relationships to weight , length , head circumference , ponderal index , and placental weight at birth in 199 normal term singletons . ^^^ IGF 2 levels correlated with levels of IGF 1 ( r = 0 . 29 ; P < 0 . 0005 ) , IGFBP 3 ( r = 0 . 45 ; P < 0 . 0005 ) , and soluble IGF2R ( r = 0 . 20 ; P < 0 . 005 ) . ^^^ Correlations between the IGFs and size at birth were stronger in nonprimiparous pregnancies ; in these , IGF 1 ( r = 0 . 52 ; P < 0 . 0005 ) , IGFBP 3 ( r = 0 . 41 ; P < 0 . 0005 ) , and the IGF 2 to IGF2R ratio ( r = 0 . 40 ; P < 0 . 0005 ) were most closely related to placental weight , together accounting for 39 % of its variance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the circulation insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) form a 150 kDa ternary complex that is of importance for the regulation of IGF 1 bioactivity . ^^^ GH administration significantly increased serum IGF 1 and IGFBP 3 levels and the IGF I / IGFBP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AAI produced in the adolescents of both sexes in our series : a decrease in growth hormone ( GH ) levels , without significant alteration of either insulin like growth factor 1 ( IGF 1 ) or insulin like growth factor binding protein 3 ( IGFBP 3 ) ; an increase in plasma glucose and a decrease in insulin in the female adolescents but not in the males . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin Like growth factor 1 ( IGF 1 ) and cyclic adenosine 3 ' , 5 ' monophosphate regulate IGF binding protein 3 gene expression by transcriptional and posttranscriptional mechanisms in mammary epithelial cells . ^^^ Insulin like growth factor 1 ( IGF 1 ) is a potent mitogen for both normal and transformed mammary epithelial cells ( MEC ) , and IGF binding protein 3 ( IGFBP 3 ) potentiates IGF 1 action in these cells . ^^^ The synthesis of IGFBP 3 is stimulated by both IGF 1 and agents that increase intracellular cAMP ( e . g . forskolin ) in the bovine MEC line MAC T . ^^^ In addition , the combination of IGF 1 and cAMP increases IGFBP 3 messenger RNA to a greater extent than does either treatment alone . ^^^ Nuclear run on assays indicated that IGFBP 3 transcription rates were increased 3 . 5 + / 0 . 83 fold ( n = 4 ) in cells treated with a combination of IGF 1 and forskolin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess the validity of the use of IGF 1 , IGFBP 1 and IGFBP 3 as biochemical markers of nutritional status in prepubertal healthy children . ^^^ RESULTS : Overweight children were found to have higher serum levels of IGF 1 ( 306+ / 162 . 2 vs . 209+ / 71 . 2 ng / ml , p < 0 . 001 ) and IGFBP 3 ( 3 . 3+ / 1 . 0 vs . 2 . 9+ / 0 . 5 mg / l , p < 0 . 01 ) and lower serum levels of IGFBP 1 ( 4 . 3+ / 3 . 9 vs . 13 . 8+ / 7 . 4 microg / l , p < 0 . 01 ) . ^^^ There was a positive correlation with BMI , for IGF 1 and IGFBP 3 , and negative correlation for IGFBP 1 . ^^^ CONCLUSION : IGF 1 , IGFBP 1 and IGFBP 3 clearly classify over and underweight prepubertal children , showing a good correlation with BMI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a previous study , weight gain , insulin growth factor 1 ( IGF 1 ) and insulin growth factor binding protein 3 ( IGFBP 3 ) were increased in rats fed suboptimal levels of energy and administered 0 . 1 mg / 100 g of body weight of recombinant human growth hormone ( rhGH ) . ^^^ After 4 wk , serum IGF 1 and IGFBP 3 , body fat , fat free mass , and total body water were determined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The growth axis of 50 individuals with a cytogenetically and molecularly confirmed 18q deletion was investigated by determining height , growth velocity , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , bone maturation , and response to pituitary stimulants of GH . ^^^ Growth factors are skewed downward ; 72 % of the IGF 1 values and 83 % of the IGF binding protein 3 values are below the mean for chronological age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ovarian follicular concentrations of activin , follistatin , inhibin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor in spontaneous menstrual cycles of normal women of advanced reproductive age . ^^^ FF was analyzed for estradiol , progesterone , testosterone , androstenedione , inhibin A and B , total activin A , total follistatin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor ( VEGF ) concentrations . ^^^ We found that the dominant follicles from older women contain normal concentrations of steroids , inhibin A and B , IGF 2 , IGFBP 2 , and IGFBP 3 ; increased concentrations of follistatin , activin A , and VEGF ; and decreased concentrations of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At baseline , we determined fasting blood levels of free and total IGF 1 , IGFBP 1 , and IGFBP 3 . ^^^ Higher total IGF 1 and the total IGF I / IGFBP 3 ratio were associated with less cognitive decline ( OR per SD increase = 0 . 65 ; 95 % CI = 0 . 44 0 . 95 and OR = 0 . 59 ; 95 % CI = 0 . 39 0 . 87 , respectively ) . ^^^ In conclusion , in this prospective study higher serum total IGF 1 levels and higher total IGF I / IGFBP 3 ratios , but not higher free IGF 1 levels , were associated with less cognitive decline over the following 2 yr . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of lipids , lipoprotein ( a ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) were also measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Within the Flexi Scope Trial ( healthy volunteers aged 55 64 years ) , at one study centre , IGF 1 and IGFBP 3 levels in serum samples collected prospectively from 442 attendants were measured . ^^^ RESULTS : Mean serum IGF 1 and IGFBP 3 levels were similar in individuals with a normal colonoscopy finding and in those with low risk adenomas . ^^^ By contrast , the mean ( s . d . ) serum IGF 1 level was increased ( 190 ( 53 ) versus 169 ( 54 ) microg / l ; P = 0 . 06 ) and the serum IGFBP 3 concentration was significantly decreased ( 3 . 22 ( 0 . 60 ) versus 3 . 47 ( 0 . 62 ) mg / l ; P = 0 . 05 ) in individuals with high risk adenomas compared with levels in those with normal colonoscopy and low risk adenomas combined . ^^^ With high risk adenoma as the dependent factor , regression models demonstrated a significant positive association with IGF 1 after controlling for IGFBP 3 ( RR per one standard deviation ( 1s . d . ) change 4 . 39 ( 95 per cent confidence interval ( c . i . ) 1 . 31 14 . 7 ) ; P = 0 . 02 ) and , independently , an inverse association with IGFBP 3 after adjustment for IGF 1 ( RR per 1s . d . change 0 . 41 ( 95 per cent c . i . 0 . 20 0 . 82 ) ; P = 0 . 01 ) . ^^^ CONCLUSION : These findings suggest that circulating IGF 1 and IGFBP 3 levels are related to future colorectal cancer risk and , specifically , may predict adenoma progression . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : These results suggest that the concomitant states of serum insulin like growth factor 1 and insulin like growth factor binding protein 3 levels in patients with chronic hepatitis may be different from cirrhotic patients and high serum IGF 1 levels may be a specific finding of the stage of chronic hepatitis before developing cirrhosis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We recently found that IGFBP 3 modulates IGF 1 binding to its receptor via a different mechanism possibly involving conformational alteration of the receptor . ^^^ MCF 7 breast carcinoma cells were preincubated with increasing concentrations of IGFBP 3 and then stimulated with IGF 1 , des ( 1 3 ) IGF 1 , or [ Q ( 3 ) A ( 4 ) Y ( 15 ) L ( 16 ) ] IGF 1 , the latter two being IGF 1 analogs with intact affinity for the type 1 IGF receptor , but weak or virtually no affinity for IGFBPs . ^^^ At 2 . 5 nM , IGFBP 3 provoked more than 50 % inhibition of the stimulation induced by 3 nM des ( 1 3 ) IGF 1 and , at 10 nM , more than 80 % inhibition . ^^^ Our results provide the first demonstration that IGFBP 3 can specifically modulate the IGF 1 signaling pathway independently of its IGF 1 binding ability . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We engineered two IGFBP selective variants that demonstrated 700 and 80 , 000 fold apparent reductions in affinity for IGFBP 1 while preserving low nanomolar affinity for IGFBP 3 , the major carrier of IGF 1 in plasma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 2 correlated positively with IGFBP 1 and negatively with IGF 1 and IGFBP 3 in normal subjects and at diagnosis of the pathologies studied . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The role of IGFs in cancer is supported by epidemiologic studies , which have found that high levels of circulating IGF 1 and low levels of IGFBP 3 are associated with increased risk of several common cancers , including those of the prostate , breast , colorectum , and lung . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Importantly , in addition to increasing IGF 1 levels , GH also increases the serum levels of IGFBP 3 and serum IGFBP 3 levels have been shown to be negatively correlated with the risk of cancer in the above mentioned epidemiological studies and in a similar study on colon cancer . ^^^ These studies suggest that cancer risk is increased in individuals in whom both high IGF 1 levels and low IGFBP 3 levels are present . ^^^ In subjects treated with GH , IGF 1 and IGFBP 3 levels both rise together and are not within the elevated cancer risk range , based on published studies . ^^^ These should take into account several factors , including the duration of exposure , the risk magnitude associated with the degree of serum IGF 1 elevation , and the adjusted risk based on a concomitant increase in IGFBP 3 levels . ^^^ Until further research in the area dictates otherwise , on going cancer surveillance and routine monitoring of serum IGF 1 and IGFBP 3 levels in GH recipients should be the standard of care . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aims of this study were to assess if hyperglycaemia in children with type 1 diabetes was associated with changes in free dissociable IGF 1 ( fdIGF 1 ) and IGF binding protein 3 protease activity ( IGFBP 3 PA ) and if increased insulin resistance during puberty was associated with changes in IGFBP 3 PA in healthy and diabetic children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , in the CRF group , especially in the younger children , low levels of IGF 1 and IGFBP 3 are evocative of an associated resistance at the GH receptor level . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Also , a close relationship between feed intake and body weight were found , together with increases in epiphyseal growth plate width , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After purification to homogeneity by IGF 1 affinity chromatography and reverse phase HPLC , sequence analysis revealed the amino terminus of IGFBP 3 , and mass spectrometry indicated a molecular mass of 12 295 Da . ^^^ IGFBP 3 [ 1 97 ] at 11 nM inhibited IGF 1 stimulated DNA synthesis by 50 60 % in MCF 7 breast cancer cells , similar to the inhibition observed with the intact protein . ^^^ In the absence of IGF 1 , DNA synthesis was inhibited by IGFBP 3 [ 1 97 ] , but not intact IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 18 healthy women , the effect of two oral contraceptives ( OCs ) on insulin like growth factor ( IGF 1 ) and its binding protein 3 ( IGFBP 3 ) and growth hormone ( hGH ) plasma level were studied before and after intake of either of two OC formulations over 21 days , one containing 2 mg dienogest and 0 . 03 mg ethinylestradiol ( group A ) and the other 0 . 125 mg levonorgestrel and 0 . 03 mg ethinylestradiol ( group B ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A rise in IGF 1 and IGFBP 3 levels was seen in each treatment group , but only the increase in IGF 1 was significant ( P=0 . 0138 , always ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The importance of routinely monitoring serum levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 is an emerging paradigm . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 expression in cultured human preadipocytes and adipocytes . ^^^ The expression and secretion of IGF 1 and IGFBP 3 were investigated in cultured human preadipocytes and in in vitro differentiated adipocytes derived from human subcutaneous adipose tissue under chemically defined culture conditions . ^^^ Human preadipocytes expressed mRNAs for IGF 1 and IGFBP 3 and secreted the corresponding proteins into the culture medium as measured by sensitive radioimmunoassays . ^^^ In human adipocytes ; specific mRNA expression was comparable to that found in preadipocytes , but IGF 1 secretion was increased 10 fold ( 3 . 87 + / 0 . 69 vs . 0 . 41 + / 0 . 11 ng / ml / 10 ( 6 ) cells / 48 hrs , p < 0 . 05 ) and IGFBP 3 secretion 2 . 5 fold ( 7 . 34+ / 1 . 15 vs . 3 . 27+ / 0 . 38 ng / ml / 10 ( 6 ) cells / 48 hrs , p < 0 . 05 ) in the presence of adipogenic medium probably resulting in an increase of unbound IGF 1 . ^^^ Under serum free , chemically defined conditions human growth hormone ( hGH ) and insulin were found to be positive regulators and cortisol was found to be a negative regulator of IGF 1 and IGFBP 3 secretion in preadipocytes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Significance of basal IGF 1 , IGFBP 3 and IGFBP 2 measurements in the diagnostics of short stature in children . ^^^ The role of IGF 1 and IGFBP 3 measurements in the diagnostic work up of short children is established but remains controversial . ^^^ In comparing IGF 1 , IGFBP 3 and IGFBP 2 serum levels of GHD and ISS children with reference data , we calculated the sensitivity , specificity , efficiency and positive predictive value for the diagnosis of GHD . ^^^ The overall sensitivity of the parameters was high , the rank order being as follows : IGF 1 > IGFBP 3 > IGFBP 2 ( 75 , 67 and 62 % , respectively ) . ^^^ In contrast , the specificity was relatively low : IGFBP 3 > IGFBP 2 > IGF 1 ( 50 , 50 and 32 % , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 values , but not IGFBP 3 values , correlated positively with dermal thickness in GHD children , before and after 1 year of GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 , IGF binding protein 3 , and acid labile subunit were measured as well . ^^^ There were no significant differences between the short stature and normal group in peak or mean GH concentrations regardless of the assay used ; however , the IGF 1 , IGF binding protein 3 , and acid labile subunit concentrations were substantially lower in the short stature group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured levels of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and insulin in plasma samples from 149 men who had a diagnosis of prostate cancer between 1 month and 10 years after blood collection and among 298 control men . ^^^ RESULTS : Case subjects had statistically significantly higher mean levels of IGF 1 than control subjects ( 229 ng / mL ; 95 % confidence interval [ CI ] = 218 240 ng / mL ] versus 214 ng / mL [ 95 % CI = 208 221 ng / mL ] ; P = . 02 ) and IGFBP 3 ( 2611 ng / mL [ 95 % CI = 2518 2704 ng / mL ] versus 2498 ng / mL [ 95 % CI = 2437 2560 ng / mL ] ; P = . 04 ) . ^^^ Conditional logistic regression analyses showed increases in prostate cancer risk with rising levels of IGF 1 ( P : ( for trend ) = . 02 ) and IGFBP 3 ( P ( for trend ) = . 03 ) . ^^^ In case subjects younger than 59 years at the time of blood collection , the risk associated with increased IGF 1 was higher ( P : ( for trend ) = . 01 ) , whereas the risk associated with increased IGFBP 3 was lower ( P ( for trend ) = . 44 ) than the corresponding risks in the full cohort . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Can measurements of IGF 1 and IGFBP 3 improve the sensitivity of prostate cancer screening . ^^^ Further well designed studies are urgently needed to clarify if use of prostate specific antigen ( PSA ) as a diagnostic test for prostate cancer can be improved by incorporating measurements of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) , and if these measurements might also identify men at higher risk . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To study the possible role of sexual hormones , Testosterone ( T ) , Estradiol ( E 2 ) , follicle stimulating hormone ( FSH ) , and luteinizing hormone ( LH ) on the growth axis , we examined the correlations between the sex , growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , FSH , LH , T and E 2 , in growth retarded children ranging in age from 7 to 13 yr . ^^^ We have found : a ) Thai only FSH concentrations were significantly higher in girls than in boys ; b ) A positive correlation between T IGF 1 ; T IGFBP 3 ; FSH LH ; FSH IGF 1 ; FSH IGFBP 3 , LH IGF 1 , LH IGFBP 3 the sex FSH ; and the sex IGFBP 3 c ) A high positive correlation between plasmatic E 2 and IGF 1 / IGFBP3 ratio ( an index of free , active IGF 1 ) . ^^^ The sex through IGFBP 3 ; LH , FSH , and T through IGF 1 and IGFBP 3 ; E 2 through the IGF 1 / IGFBP3 ratio , an index of active IGF 1 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conclude that short term administration of ibutamoren mesylate can increase GH , IGF 1 , and IGFBP 3 levels in some children with GH deficiency . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition ( dual energy 10 ray absorptiometry ) , resting VO 2 ( indirect calorimetry ) , resting HR ( telemetry ) and serum IGF 1 , IGF 2 , IGFBP 3 and acid labile subunit ( ALS ) were measured at baseline , after 12 weeks of GH administration and , additionally in the GH group , 1 , 2 , 3 , 4 , 5 and 9 days after GH discontinuation . ^^^ Resting VO 2 and resting HR increased by 31 + / 3 . 6 % and 7 . 3 + / 1 . 9 per minute , respectively , in the GH group , where significant increases in serum IGF 1 , IGFBP 3 and ALS also were noted . ^^^ CONCLUSIONS : The increases in serum IGF 1 , IGFBP 3 , ALS and resting heart rate induced by 12 weeks of GH administration in elderly men returned to baseline levels within 2 3 days after GH discontinuation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : BMD at the lumbar spine and femoral neck at baseline and after a mean follow up time of 3 . 3 years , serum IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , sex hormone binding globulin ( SHBG ) and biologically available testosterone ( BAT ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effects of IGF 1 and 2 , IGF binding protein 3 ( IGFBP 3 ) , and transforming growth factor beta ( TGF beta ) on growth and apoptosis of human osteosarcoma Saos 2 / B 10 cells : lack of IGF independent IGFBP 3 effects . ^^^ DESIGN : DNA synthesis and apoptosis in Saos 2 / B 10 cells were measured in response to IGF 1 , IGF 2 , IGFBP 3 and TGF beta 2 , and to type 1 IGF receptor ligands with poor affinity for IGFBP 3 ( [ QAYL ] IGF 1 and insulin ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) 1 , IGF 2 , free IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 and IGFBP 3 were measured by radioimmuno / immunoradiometric assays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , as a likely consequence of intraportal insulin deficiency , patients with type 1 DM also exhibit abnormalities of the growth hormone ( GH ) / IGF / IGF binding protein ( IGFBP ) axis , including GH hypersecretion , reduced circulating levels of insulin like growth factor 1 ( IGF 1 ) and IGFBP 3 , and elevated levels of IGFBP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The peritoneal fluid ( PF ) of women with endometriosis contains protease ( s ) activity able to hydrolyze insulin like growth factor binding protein 3 ( IGFBP 3 ) , increasing the bioavailability of insulin like growth factor 1 ( IGF 1 ) locally . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
STUDY DESIGN : We studied the effects of 36 h fasting on 8 h diurnal mean GH , insulin and glucose concentrations ( mGHc , mINSc and mGLUc ; assay every 30 min from 8 . 00 am to 4 . 00 pm ) as well as on IGF 1 , IGFBP 3 , ALS , IGFBP 1 , GHBP and free fatty acid ( FFA ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma was collected weekly from day 0 through day 58 , and glucose , triglycerides , cholesterol , insulin , IGF 1 , and IGF BP 3 levels were measured . ^^^ Insulin , IGF 1 , IGF BP 3 , cholesterol , body weight , and food consumption were not changed by DHEA PC treatment ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Inhibition of ERK and / or PI3K pathways mediated the inhibitory effects of cAMP on insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 gene expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of lycopene , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and prostate specific antigen were measured at baseline and after 3 weeks of supplementation or observation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Cachectic patients showed an increase of total GH and immunologically intact GH ( p < or = 0 . 0002 ) and a decrease of GH binding protein ( BP ) ( p = 0 . 005 ) , IGF BP 3 ( p = 0 . 01 ) and IGF 1 ( p = 0 . 06 ) , compared with noncachectic patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before therapy , IGF 1 and IGF binding protein 3 serum levels of all affected children were severely diminished ( < < 5th percentiles for age ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No differences were noted in GH pulse frequency , GH pulse amplitude , IGF 1 , IGF binding protein 1 , and IGF binding protein 3 before and after treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To measure circulating concentration of growth hormone ( GH ) , insulin like growth factors 1 ( IGF 1 ) , and it ' s binding protein 1 ( IGFBP 1 ) and binding protein 3 ( IGFBP 3 ) in patients with sever hepatitis , and to survey the clinical significance of GH IGFS axis . ^^^ Serum concentration of GH , IGF 1 , IGFBP 1 and IGFBP 3 was determined by ELISA . ^^^ The positive relationship between IGF 1 and IGFBP 3 ( r=0 . 91 , P < 0 . 001 ) was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 by immunoassays , IGFBP 3 by Western ligand blotting ( WLB ) and finally in vitro IGFBP 3 proteolysis by a ( 125 ) 1 IGFBP 3 degradation assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma renin activity , thyroid function tests , parathyroid hormone , prolactin , IGF 1 , IGFBP 3 concentrations and serum electrolytes were normal . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND PATIENTS : To this goal , we studied the effect of 4 day treatment with 3 rhGH doses ( 1 . 25 , 2 . 5 and 5 . 0 microg / kg / day ) on IGF 1 and IGFBP 3 levels in 25 panhypopituitary adults with severe GHD ( 12 males and 13 females , age : 44 . 5+ / 3 . 0 years , body mass index ( BMI ) : 27 . 0+ / 0 . 9 kg / m ( 2 ) ) and 21 normal young adult volunteers ( NV , 12 males and 9 females , age : 30 . 5+ / 2 . 0 years , BMI : 20 . 8+ / 0 . 5 kg / m ( 2 ) ) . ^^^ RESULTS : Basal IGF 1 and IGFBP 3 levels in GHD were lower ( P < 0 . 001 ) than in NV . ^^^ CONCLUSION : The results of the present study demonstrate that the minimal rhGH dose able to increase IGF 1 and IGFBP 3 levels in GHD patients is lower than in normal subjects , at least after a very short treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Elevated levels of circulating GH , increased GH secretory amplitude , and decreased concentrations of IGF 1 , IGFBP 3 , and GHBP have been related to poor glycemic control . ^^^ Twenty four hour every 20 minute blood sampling for GH determination was analyzed using the Cluster algorithm , and static measures of IGF 1 , IGFBP 3 , and GHBP were obtained . ^^^ Serum levels of IGF 1 and IGFBP 3 were greater in the mid pubertal boys , but levels of GHBP were higher in the prepubertal boys . ^^^ No differences were found among the 3 groups for levels of IGF 1 , IGFBP 3 , or GHBP . ^^^ We conclude that normal increases in GH secretion and levels of IGF 1 and IGFBP 3 occur during mid puberty in boys with IDDM . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this report , the effect of ACEI on CD34+ erythroid precursor apoptosis was studied , as were hematocrit ( Hct ) , Ep , IGF 1 , and IGF binding protein 3 ( IGF BP 3 ) levels . ^^^ At the same time , Hct , Ep , IGF 1 , and IGF BP 3 levels were measured . ^^^ Baseline CD34+ cell number , CD34+ apoptosis , Ep , IGF 1 , and IGF BP 3 levels were the same between PTE and transplant control subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 ( IGFBP 3 ) mRNA was highest in CFP and MFP whereas the protein products were highest in PAR . ^^^ A local increase in the mammary IGF 1 : IGFBP 3 ratio mediates the mammogenic effects of estrogen and growth hormone . ^^^ Increased proliferation of mammary parenchymal cells was associated with increased IGF 1 and reduced IGFBP 3 protein in mammary tissue . ^^^ An increase in the ratio of mammary IGF 1 : IGFBP 3 likely increases the proportion of the mammary IGF 1 available to stimulate proliferation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Estrogen appeared to have a suppressive effect on the insulin like growth factor ( IGF ) system such that plasma concentration of IGF binding protein 3 was significantly reduced and plasma levels of estradiol and IGF 1 were negatively correlated during estrogen treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recent encouraging data using the IGF 1 IGF binding protein 3 ( IGFBP 3 ) complex in diabetes mellitus suggest that this preparation may eventually replace recombinant free IGF 1 . ^^^ It will probably be decided during the next decade whether use of IGF 1 or the IGF 1 IGFBP 3 complex becomes firmly established as an accepted endocrine therapy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study tested the link between serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels and body height , nutritional status , pulmonary function tests and activity of inflammation in 92 subjects with CF ( age 2 . 1 18 . 8 y ) . ^^^ It also analysed the effect of short term antibiotic treatment and hyperalimentation on IGF 1 and IGFBP 3 levels in 33 subjects ( age 3 . 6 33 . 7y ) on 41 occasions . ^^^ Both IGF 1 ( 1 . 19 + / 0 . 17 SD ) and IGFBP 3 levels ( 0 . 66 + / 0 . 12 SD ; both p < 0 . 0001 vs 0 ) were decreased in cross sectional measurements . ^^^ Their standardized values were inversely proportional to age ( IGF 1 : r = 0 . 23 , p = 0 . 03 ; IGFBP 3 : r = 0 . 29 , p = 0 . 005 ) and positively correlated with SDS of height ( IGF 1 : r = 0 . 40 , p < 0 . 0001 ; IGFBP 3 : r = 0 . 36 , p = 0 . 0005 ) and of mid arm circumference ( IGF 1 : r = 0 . 39 , p = 0 . 0001 ; IGFBP 3 : r = 0 . 38 , p = 0 . 0002 ) , and with pulmonary function tests . ^^^ After a short term course of intensive antibiotic therapy and hyperalimentation , IGF 1 normalized ( from 0 . 66 + / 0 . 20 to 0 . 00 + / 0 . 25 SD ; p < 0 . 0001 ) and IGFBP 3 increased ( from 0 . 78 + / 0 . 15 to 0 . 53 + / 0 . 16 SD ; p = 0 . 002 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also determined serum levels of IGF 1 , IGFBP 3 and osteocalcin ( OC ) as well as urinary levels of deoxy pyridinoline ( D Pyr ) and CrossLaps . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Efficacy of radiotherapy in normalizing serum IGF 1 , acid labile subunit ( ALS ) and IGFBP 3 levels in acromegaly . ^^^ Serum GH and IGF 1 levels off medical therapy were measured in all patients ; ALS and IGFBP 3 were measured in 11 patients with normalization of IGF 1 concentrations . ^^^ ALS and IGFBP 3 concentrations paralleled IGF 1 levels in all patients studied . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A high ratio of IGF 1 to IGF binding protein 3 ( IGFBP 3 ) has been linked to an increased risk of colorectal cancer . ^^^ Circulating levels of IGF 1 and IGFBP 3 were assayed among 193 men who developed colorectal cancer during 13 years of follow up and 318 age and smoking matched cancer free control men . ^^^ Intake of dairy food ( especially low fat milk ) was also positively and moderately associated with plasma levels of IGF 1 , IGFBP 3 , and IGF I / IGFBP 3 among control men . ^^^ CONCLUSION : Intake of dairy products was associated with a modest increase in circulating IGF 1 levels , but intake of low fat milk was associated with lower risk of colorectal cancer , particularly among individuals with high IGF I / IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood levels of IGF 1 and IGFBP 3 were reduced before the diet in all patients while ligand blotting showed that IGFBP 2 and 1 were increased . ^^^ Furthermore , we found a significant , positive correlation between GH BP 2 and IGF 1 or IGFBP 3 levels . ^^^ The body mass index significantly and positively correlated with GH BP 2 , IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Their height was 3 . 0 + / 0 . 7 SDS ; IGF 1 , 1 . 2 + / 1 . 1 SD ; IGF binding protein 3 , 0 . 7 + / 2 . 0 SDS ; and GH binding protein , 0 . 4 + / 0 . 8 SDS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relevance of IGF 1 , IGFBP 3 , and IGFBP 2 measurements during GH treatment of GH deficient and non GH deficient children and adolescents . ^^^ RESULTS : In prepubertal GHD children , the levels of IGF 1 , IGFBP 3 , and IGF I / IGFBP 3 exceeded the 95th centile of the reference values for this age group in 2 . 3 , 0 . 3 , and 7 . 9 % of the cases , respectively , whereas in prepubertal non GHD children , the same parameters exceeded the 95th reference centile in 20 . 1 , 3 . 5 , and 32 . 2 % , respectively . ^^^ In pubertal GHD children IGF 1 , IGFBP 3 , and IGF I / IGFBP 3 levels exceeded the 95th reference centile in 11 . 1 , 1 . 5 , and 15 . 4 % , respectively . ^^^ Our analysis showed that , in both groups , in addition to maximum GH , all IGF parameters ( IGF 1 , IGFBP 3 , IGF I / IGFBP 3 ratio , IGFBP 2 or derivatives ) significantly extend the scope of a calculated model for predicting 1st year height velocity . ^^^ CONCLUSION : For reasons of safety and optimization of GH therapy , it is essential to follow up IGF 1 , IGFBP 3 , and IGFBP 2 levels regularly during childhood . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Classical growth hormone insensitivity syndrome ( GHIS ) comprises a dysmorphic phenotype , extreme short stature ( height SDS < 3 ) , normal GH and low IGF 1 and IGFBP 3 . ^^^ Classical and atypical GHIS groups were compared in terms of ( 1 ) phenotypic features , including high pitched voice , sparse hair , blue sclera , hypoglycaemia , microphallus , ( 2 ) birth length , height SDS , and ( 3 ) basal IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , GHBP and increase in IGF 1 on IGF 1 generation testing . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The results of GH provocative tests were compared to age and gender based standard deviation scores ( SDS ) of plasma IGFBP 2 , IGF 1 , IGFBP 3 and the molar ratios of the latter two to IGFBP 2 . ^^^ The use of various combinations of IGFBP 2 , IGF 1 , IGFBP 3 and the derived ratios only slightly improved the diagnostic efficiency as compared to the results of the individual tests . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study was performed to analyze the correlations of circulating levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 with biochemical markers of bone metabolism and parameters of the urea kinetic model which reflect nutritional conditions in hemodialysis patients . ^^^ We measured the serum levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Biophysical and biochemical data show that the detergent binds to IGF 1 specifically and blocks binding of IGFBP 1 and IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin infusion increases levels of free IGF 1 and IGFBP 3 proteolytic activity in patients after surgery . ^^^ Serum levels of total IGF 1 ( tIGF 1 ) , free IGF ( fIGF ) 1 , fIGF 2 , and IGF binding protein ( IGFBP ) 1 and IGFBP 3 proteolytic activity ( IGFBP 3 PA ) , determined on the day before surgery and on the 1st postoperative day , were related to insulin sensitivity measured by a hyperinsulinemic , normoglycemic clamp . ^^^ We propose that , in catabolic , postoperative patients , increased levels of insulin from exogenous or , possibly , endogenous sources ( nutritionally induced ) may be a signal to increase IGF 1 bioavailability by increased expression of IGFBP 3 PA to counteract further deterioration in glucose metabolism . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to determine the value of serum insulin like growth factor binding protein 3 ( IGFBP 3 ) and serum leptin measurements in comparison with plasma insulin like growth factor 1 ( IGF 1 ) measurements as indicators of treatment success in patients with acromegaly . ^^^ We comparatively analyzed plasma IGF 1 , serum IGFBP 3 , and serum leptin levels in the aforementioned groups . ^^^ As expected , the patients with active acromegaly had significantly higher mean values of plasma IGF 1 and serum IGFBP 3 and lower mean values of serum leptin ( only in women ) than the control group . ^^^ However , individual evaluation showed that 1 of 20 , 9 of 20 , and many patients with postadenomectomy active acromegaly patients had values that overlapped values of control subjects for plasma standard deviation score ( SDS ) IGF 1 , serum SDS IGFBP 3 , and sex adjusted serum leptin , respectively . ^^^ Application of the ROC curves method and the determination of positive and negative likelihood ratios in comparative evaluation of serum IGFBP 3 and serum leptin with plasma IGF 1 as indicators of treatment success in acromegalic patients showed that neither serum IGFBP 3 nor serum leptin determinations have accuracy better than or similar to that of plasma IGF 1 for monitoring treatment success in acromegaly patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels in serum and the IGF 1 levels in liver were similar in all groups . ^^^ In the tibia no significant differences were observed in IGF 1 , IGFBP 3 and TGFbeta concentration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Growth parameters and plasma concentrations of growth hormone ( GH ) , IGF 1 , and IGFBP 3 were studied in 41 children with sickle cell anemia whose haplotypes were defined . ^^^ RESULTS : Plasma concentrations of IGF 1 ( total , free , and free / total fraction ) and IGFBP 3 were significantly reduced in all patients with sickle cell anemia compared with the healthy children . ^^^ A positive correlation was found between hematocrit and total IGF 1 and between fetal hemoglobin percentages and the z scores for total IGF 1 and IGFBP 3 . ^^^ CONCLUSIONS : The positive relationship between hematocrit and fetal hemoglobin percentages with total IGF 1 , free / total IGF 1 , and IGFBP 3 in patients with sickle cell anemia could show that the delayed growth of these patients may be linked to intrinsic factors of the disease , which also determine the low circulating concentrations of the various elements of the GH / IGF 1 axis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The ternary complex , composed of IGF 1 or IGF 2 , IGF binding protein 3 , and the acid labile subunit , is responsible for transport of the majority of the IGF 1 and IGF 2 present in the circulation . ^^^ There were no significant differences in serum IGF binding protein 3 or IGF 1 levels between transgenic and wild type mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin , IGF 1 and IGF 2 brought about a 1 . 5 2 fold enhancement of IGFBP 3 release at 7 . 5 30 nM concentrations . ^^^ We conclude that , in addition to GH , also insulin , IGF 1 and IGF 2 and glucocorticoids can modulate IGFBP 3 secretion by human hepatoma cells . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The responses of IGFBP 1 and IGFBP 3 to increased plasma IGF 1 and insulin may serve to protect the fetus from exaggerated anabolic effects and to blunt the hypoglycemic potential of circulating IGFs and insulin . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentrations of serum free IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were also shown to be significantly affected by equine and bovine GH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to compare concentrations of IGF 1 , IGF binding protein 1 ( IGF BP 1 ) , and IGF BP 3 in patients with breast cancer ( n = 14 ) with those seen in control subjects ( n = 23 ) and to assess the effect of tamoxifen on IGF status in these patients . ^^^ RESULTS : IGF 1 , IGF BP 3 , and IGF BP 1 concentrations were not significantly different in cases and controls . ^^^ However , the IGF 1 to IGF BP 3 ratio was significantly decreased at both nine and 18 months ( baseline : mean , 0 . 058 ; SD , 0 . 014 ; nine months : mean , 0 . 039 ; SD , 0 . 008 ; p = 0 . 033 ; 18 months : mean , 0 . 044 ; SD , 0 . 012 ; p = 0 . 01 ) . ^^^ It also decreases the IGF 1 to IGF BP 3 ratio but this effect may be limited after long term use . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effects of an endurance cycling competition on resting serum insulin like growth factor 1 ( IGF 1 ) and its binding proteins IGFBP 1 and IGFBP 3 . ^^^ Serum levels of both total and free IGF 1 and IGF binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) were measured in each of the samples . ^^^ A significant ( p < 0 . 01 ) decrease was noted in free IGF 1 at t ( 3 ) compared with both t ( o ) and t ( 1 ) ( t ( 0 ) : 0 . 9 ( 0 . 1 ) ng / ml ; t ( 1 ) : 0 . 9 ( 0 . 1 ) ng / ml ; t ( 3 ) : 0 . 7 ( 0 . 1 ) ng / ml ) ; in contrast , IGFBP 3 levels remained stable throughout the race . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The influence of nutritional status and hormonal growth activity on the impaired somatic development of adolescents with the hepatosplenic clinical form of Schistosoma mansoni infection ( HS ) , the intestinal form with high ( IH ) or low ( IL ) egg output and non infected ( NI ) individuals was evaluated ( in Comercinho , Minas Gerais State , Brazil , in 1996 97 ) by measuring body mass index ( BMI ) , insulin like growth promoting factor ( IGF 1 ) and its carrier protein ( IGFBP 3 ) . ^^^ BMI , IGF 1 and IGFBP 3 concentrations were significantly lower in the HS group compared with the IH and the NI groups , irrespective of age . ^^^ BMI did not remain associated with the clinical form in the bi variate model that included IGF 1 and BMI or IGFBP 3 and BMI , suggesting that in these groups IGF 1 and IGFBP 3 levels were related to the clinical form but independent of nutritional status . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circadian rhythms of basic fibroblast growth factor ( bFGF ) , epidermal growth factor ( EGF ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , cortisol , and melatonin in women with breast cancer . ^^^ METHODS : Circadian variations were studied in cortisol , melatonin , cytokines ( basic fibroblast growth factor IbFGF ] , EGF , insulin like growth factor 1 [ IGF 1 ] ) , and a cytokine receptor ( insulin like growth factor binding protein 3 [ IGFBP 3 ] ) in the plasma of 28 patients with metastatic breast cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with controls and after adjustment for baseline , treatment with fenretinide for 1 year induced the following changes : IGF 1 , 13 % [ 95 % confidence interval ( CI ) , 25 to 1 % ] in women < or = 50 years of age and 3 % ( 95 % CI , 16 to 13 % ) in women > 50 years of age ; IGFBP 3 , 4 % ( 95 % CI , 12 to 6 % ) in both age groups ; IGF 1 : IGFBP 3 molar ratio , 11 % ( 95 % CI , 22 to 1 % ) in women < or = 50 years of age and 1 % ( 95 % CI , 11 to 16 % ) in women > 50 years of age . ^^^ Drug and metabolite concentrations were negatively correlated with IGF 1 and IGF 1 : IGFBP 3 molar ratio in women < or = 50 years of age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND / AIMS : In healthy adults , serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) form a 150 kDa ternary complex under the control of growth hormone ( GH ) . ^^^ METHODS : Serum IGF 1 , IGFBP 3 , ALS , the 150 kDa ternary complex and IGFBP 3 proteolysis were all measured in six compensated and six decompensated cirrhotic patients and compared to six healthy controls . ^^^ RESULTS : Patients with compensated cirrhosis had decreased levels of IGF 1 ( 55 % ) , IGFBP 3 ( 64 % ) and ALS ( 53 % ) , and in the decompensated patients these levels were decreased even further : IGF 1 ( 32 % ) , IGFBP 3 ( 37 % ) and ALS ( 27 % ) compared to healthy controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Maternal and fetal serum insulin like growth factor 1 ( IGF 1 ) IGF binding protein 3 ( IGFBP 3 ) , leptin levels and early postnatal growth in infants born asymmetrically small for gestational age . ^^^ This study was planned to investigate the relationship between birth weight and insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and leptin levels in neonates with normal growth ( appropriate for gestational age : AGA ) and retarded growth ( small for gestational age : SGA ) ; and to evaluate these growth factors ' effects in early postnatal growth . ^^^ IGF 1 , IGFBP 3 and leptin levels were measured in maternal serum and venous cord blood at birth and at 15 days of life of neonates using specific RIAs . ^^^ Maternal serum IGF 1 , IGFBP 3 and leptin levels did not show correlations with birth weight . ^^^ In contrast , there were significantly positive correlations between birth weight and venous cord blood IGF 1 , IGFBP 3 and leptin levels ( p < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also studied the serum concentrations of IGF 1 and IGFBP 3 in relation to leptin concentrations . ^^^ The mean concentrations of leptin , IGF 1 and IGFBP 3 were 1 . 63 + / 1 . 09 , 24 . 65 + / 10 . 04 and 976 . 05 + / 214 . 50 , respectively , at birth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Hedges ' standardized mean difference ( HSMD ) and odds ratio ( OR ) were used to estimate the effect of IGF 1 and IGF binding proteins ( IGFBP 3 ) . ^^^ Circulating levels of IGF 1 and IGFBP 3 are likely to be higher in prostate cancer patients than in the controls . ^^^ These findings support the suggestion that high IGF 1 and IGFBP 3 are associated with an increased risk of prostate cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , and IGFBP 3 in white and African American men at increased risk of prostate cancer . ^^^ OBJECTIVES : To further investigate the relationship between the plasma levels of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , growth hormone , testosterone , and demographic factors , particularly race , within a group of men at increased risk of prostate cancer development . ^^^ METHODS : Enzyme linked immunosorbent assays or an immunosorbent assay was used to quantitate the plasma levels of IGF 1 , IGF 2 , IGFBP 3 , growth hormone , and testosterone . ^^^ Overall , the IGF 1 plasma levels correlated positively with the IGF 2 , IGFBP 3 , and growth hormone levels and the IGF 2 plasma levels correlated negatively with the testosterone levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 increased significantly after recombinant human GH treatment ( +24 % and +58 % , respectively ) compared with control values ( 14 % and +5 % ; P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RIA and RT PCR demonstrated that the genital skin fibroblast strains expressed IGF 2 , IGF binding protein 2 , and IGF binding protein 3 , but no IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , the ability of the IGF binding protein 3 mutants to inhibit IGF 1 stimulated phosphorylation of its receptor was a reflection of their affinity for IGF , with the lowest affinity mutants having the least inhibitory effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Here we show that NSE / hIL 6 mice have normal liver IGF 1 production , decreased levels of IGF binding protein 3 ( IGFBP 3 ) and increased serum IGFBP 3 proteolysis . ^^^ Patients with systemic juvenile idiopathic arthritis ( s JIA ) , a chronic inflammatory disease characterized by prominent IL 6 production and complicated by stunted growth associated with low IGF 1 levels , have markedly decreased IGFBP 3 levels , increased serum IGFBP 3 proteolysis and normal acid labile subunit levels . ^^^ Our data show that chronic overproduction of IL 6 causes decreased IGFBP 3 levels , resulting in a decreased association of IGF 1 in the 150 kDa complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As in mKlk 21 , IGF 1 and IGFBP 3 were expressed in the Leydig cells of the adult mouse testis , although the transcript of IGFBP 3 was not detected in all of the observed cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) were measured by radioimmunoassay . ^^^ RESULTS : Serum IGF 1 ( p < 0 . 001 ) , IGFBP 3 ( p < 0 . 001 ) , and the BP 3 : total IGF molar ratio ( p < 0 . 001 ) were depressed in both patient groups relative to controls . ^^^ Habitual exercise level , but not body composition , was shown to be a significant predictor for IGF 1 , IGFBP 3 , and BP 3 : total IGF molar ratio ( p < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were measured in healthy adults and acromegalics . ^^^ RESULTS : For acromegalic patients , GH was significantly correlated with IGF 1 ( r = 0 . 50 ; P < 0 . 001 ) and IGFBP 3 ( r = 0 . 29 ; P = 0 . 03 ) but not IGF 2 or IGFBP 2 . ^^^ The ( IGF 1 + IGF 2 ) / IGFBP 3 molar ratios were remarkably constant in both healthy adults and acromegalics , but the relationships of the ligands individually with IGFBP 3 were not linear : as IGFBP 3 increased , IGF 1 also increased whereas IGF 2 initially increased but then decreased . ^^^ The ( IGF 1 + IGF 2 ) / IGFBP 3 molar ratio appears to be pivotal in determining IGF 2 values , which , in turn , expressed as a ratio of IGFBP 3 , is related to IGFBP 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Biochemical features consist of severe insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) deficiency and elevated GH secretion . ^^^ GH sensitivity , measured by IGF 1 and IGFBP 3 responses in the IGF 1 generation test , may reveal abnormalities in ISS , although it is likely that the dose of recombinant human GH and frequency of sampling in the test need to be modified . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The differences between the metabolic effects of GH and IGF 1 are not fully understood , while measurements of IGF 1 and IGF binding protein 3 are perhaps not the ideal means of diagnosing GHI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Candidate biochemical markers include insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) , and markers of bone remodelling . ^^^ Serum IGF 1 has the greatest utility during GH dose titration as it is more sensitive to changes in GH status than IGFBP 3 and ALS , and is also more sensitive to excessive GH replacement . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 levels vary with age after puberty , and this is complicated by the maintenance of IGF binding protein 3 levels by IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Indeed , researchers have shown that IGF 1 levels are of greater diagnostic value than other possible markers , such as IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ Post diagnosis , monitoring of IGF 1 and IGFBP 3 levels can be used to assess the efficacy and safety of GH replacement therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , inhibitors of IGF binding proteins ( IGFBPs ) , especially IGFBP 3 , could potentially alter the distribution between the `` free ' ' and `` bound ' ' forms of IGF and thereby elevate biologically active IGF 1 to exert a beneficial effect on those patients with diseases that respond to the application of exogenous IGF 1 . ^^^ More importantly , both compounds were shown to be able to release biologically active IGF 1 from the IGF I / IGFBP 3 complex . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : The alterations of IGF 1 , IGFBP 1 and 4 observed in catabolism correlate with the effects of IL 6 on the biosynthesis of these components in primary rat liver cells , while a neutral IGFBP 3 protease was not detectable . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The findings indicate that the expression of IGFBP 3 to 6 is higher in the immature intestine compared with the adult intestine , suggesting locally produced IGFBPs may inhibit systemically derived IGF 1 action in the intestine . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The release of IGFBP 3 , progesterone , oxytocin , IGF 1 and prostaglandins F ( PGF ) and E ( PGE ) by control and transfected cells was compared . ^^^ The concentration of IGFBP 3 produced was assessed using ligand and western blotting and secretion of progesterone , oxytocin , IGF 1 , PGF and PGE was evaluated using RIA / IRMA techniques . ^^^ The granulosa cells transfected to overexpress IGFBP 3 had an increase in IGF 1 , PGF and PGE release , and a decrease in basal and hormone or growth factor induced accumulation of progesterone and oxytocin . ^^^ IGFBP 3 is a physiological stimulator of IGF 1 and prostaglandin release and an inhibitor of steroid and peptide hormone output . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Onset of significant GH dependence of serum IGF 1 and IGF binding protein 3 concentrations in early life . ^^^ Blood samples were taken at birth and at 6 , 10 , 12 , and 18 mo after birth for serum GH binding protein , IGF 1 , and IGF binding protein 3 analysis . ^^^ However , a significant rise in serum IGF 1 and IGF binding protein 3 levels was only observed from 10 mo of life onward . ^^^ A significant correlation between a change in serum IGF 1 and IGF binding protein 3 levels was observed during the three 6 mo periods between birth and 18 mo , but a significant correlation between a change in serum GH binding protein and a change in serum IGF 1 or IGF binding protein 3 levels was only seen between 12 and 18 mo of age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Despite the presence of severe GH deficiency associated with persistently low IGF 1 and IGFBP 3 levels , the patient grew spontaneously at an accelerated rate for a prepubertal boy , achieving a height velocity of 9 . 0 cm during the first and 8 . 5 cm during the second post operative year . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) binds IGF 1 and IGF 2 with high affinity , at least an order of magnitude higher than the affiniy of the IGFs for the IGFIR . ^^^ IGFBP 3 inhibited IGF 1 , IGF 2 , Des ( 1 3 ) IGF 1 and Long ( R 3 ) IGF 1 induced IGFIR phosphorylation in a dose dependent manner at similar concentration range but not QAYL induced IGFIR P . ^^^ The ( ( 1 97 ) ) IGFBP 3 fragment was able to inhibit only IGF 1 induced IGFIR P . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) binds IGF 1 and IGF 2 with high affinity , at least an order of magnitude higher than the affiniy of the IGFs for the IGFIR . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In normal mice fasting IGF 1 and the IGFBP 3 ternary complex were 2 fold higher in males than in females . ^^^ Compared to wild type , male PPARalpha / mice had 40 % lower total fasting IGF 1 concentrations , decreased ALS and less IGFBP 3 ternary complex formation , but within 4 h of refeeding there was an increase in IGF 1 and IGFBP 3 ternary complex to values similar to controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The GH treated animals showed a significant increase in total IGF 1 , free IGF 1 and IGFBP 3 after surgery ( P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aims of this study on porcine ovarian granulosa cells were to examine the effect of GH on oxytocin ( OT ) , IGF 1 and IGF 1 receptors , IGF binding protein 3 ( IGFBP 3 ) , progesterone and prostaglandin E ( PGE ) , as well as to determine whether IGF 1 and / or OT may be mediators of GH action . ^^^ The secretion of IGF 1 , OT , IGFBP 3 , progesterone and PGE was determined using RIA / IRMA , whilst the IGF 1 binding sites were measured using a radioreceptor assay . ^^^ Immunoneutralization of IGF 1 by antiserum against IGF 1 inhibited PGE secretion , but it did not influence progesterone or IGFBP 3 secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ratios of IGF 1 , IGF binding protein 3 , and prostate specific antigen in prostate cancer detection . ^^^ In this study , we sought to determine the effect on the prediction of future prostate cancer occurrence by incorporating ratios of total and free PSA , IGF 1 , IGFBP 3 into PSA testing . ^^^ Within a population based prospective cohort study , we investigated the validity ( sensitivity and specificity ) of plasma concentrations of total and free PSA , IGF 1 , and IGFBP 3 and combinations thereof , in 114 cases and 97 controls , in the range of 1 . 75 13 . 5 microg / l for PSA , as used by Khosravi et al . ( See Ref . 7 ) . ^^^ Validity estimated by the area under the curve in receiver operator characteristics analysis ( with 95 % confidence interval ) for total PSA was 0 . 78 ( range , 0 . 71 0 . 84 ) ; total / free PSA , 0 . 69 ( range , 0 . 62 0 . 76 ) ; total PSA / IGF 1 , 0 . 72 ( range , 0 . 65 0 . 79 ) ; free PSA / IGF 1 , 0 . 55 ( range , 0 . 48 0 . 63 ) ; total PSA / IGFBP 3 , 0 . 74 ( range , 0 . 68 0 . 81 ) ; and free PSA / IGFBP 3 , 0 . 57 ( range , 0 . 49 0 . 64 ) . ^^^ Analysis of ratios of IGF 1 , IGFBP 3 , and free and total PSA did not improve validity of PSA testing in the prediction of future occurrence of prostate cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 and IGFBP 3 in prediagnostic serum : association with colorectal cancer in a cohort of Chinese men in Shanghai . ^^^ This is the first study to investigate the associations of IGF 1 , IGF 2 and IGFBP 3 concentrations with the risk of colorectal cancer in prospectively collected blood samples from an Oriental population . ^^^ IGF 1 , IGF 2 and IGFBP 3 were measured in the serum of 135 men who developed colorectal cancer over 12 years of follow up and 661 control subjects drawn from the cohort , who were matched to the index cases by neighbourhood of residence , age , and year and month of sample collection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Because IGF 1 receptor binding is affected by IGF binding proteins ( IGFBPs ) , we examined how pH impacted IGFBP 3 regulation of IGF 1 . ^^^ IGF 1 binding in the absence of IGFBP 3 was diminished at reduced pH . ^^^ Addition of IGFBP 3 reduced IGF 1 cell binding at pH 7 . 4 but increased surface association at pH 5 . 8 . ^^^ This increase in IGF 1 binding at pH 5 . 8 corresponded with an increase in IGFBP 3 cell association . ^^^ This , however , was not due to an increase in affinity of IGFBP 3 for heparin at reduced pH although both heparinase 3 treatment and heparin addition reduced IGFBP 3 enhancement of IGF 1 binding . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum VEGF , free and total IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 3 , and insulin levels were measured by specific immunoassays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor type 1 ( IGF 1 ) and IGF binding protein 3 in patients with Ewing sarcoma family of tumors . ^^^ The current studies were performed to determine the levels of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) in patients with ESFT . ^^^ METHODS : The authors measured serum levels of IGF 1 and IGFBP 3 by using a radioimmunoassay from 111 patients with ESFT with a median follow up of 13 years from diagnosis . ^^^ IGFBP 3 is known to sequester IGF 1 ; the ratios of IGFBP 3 to IGF 1 were evaluated . ^^^ Patients with metastatic disease to any site had higher IGFBP 3 to IGF 1 ratios than patients with localized disease ( p 2 = 0 . 0067 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant differences in IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 , IGFBP 3 , soluble IGF 2 receptor levels ( IGF2R ) , IGF II / IGF2R ratio , and relative amounts of IGFBP 3 circulating forms were found between CG and NCG children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To provide further insight into the effect of tamoxifen at low doses on the IGF system , we have correlated the drug serum levels attained after 2 months of either placebo ( n = 32 ) , tamoxifen 20 mg / day ( n = 26 ) , 10 mg / day ( n = 23 ) or 10 mg / every other day ( n = 29 ) with the changes in IGF 1 , Insulin like Growth Factor 2 ( IGF 2 ) , Insulin like Growth Factor Binding Protein 1 ( IGFBP 1 ) , Insulin like Growth Factor Binding Protein 3 ( IGFBP 3 ) , and IGF I / IGFBP 3 ratio . ^^^ Compared with placebo , tamoxifen induced a mean + / standard error ( SE ) reduction of IGF 1 of 16 . 9 + / 7 . 8 % , p < 0 . 05 , a non significant increase of 22 . 9 + / 12 . 2 % in IGF 2 , an increase in IGFBP 1 of 49 . 3 + / 22 . 7 % , p < 0 . 05 , and a non significant change of IGFBP 3 ( 4 . 0 % + / 9 . 2 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thyroid hormones play a role in the regulation of insulin like growth factor type 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) expression , and both IGF 1 and IGFBPs have been shown to be related to the function and growth of the thyroid . ^^^ Our aim was to evaluate serum concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 in patients with thyroid dysfunction before and after normalization of thyroid function . ^^^ Serum growth hormone ( GH ) , insulin , IGF 1 , IGFBP 1 , and IGFBP 3 were measured in all patients before and after normalizing serum thyroid hormone concentrations . ^^^ Hyperthyroid patients showed IGF 1 ( 198 . 8 + / 17 . 0 microg / L ) and IGFBP 3 levels ( 4 . 2 + / 0 . 2 mg / L ) similar to those found in the control group ( 217 . 9 + / 20 . 3 microg / L and 4 . 2 + / 0 . 3 mg / L , respectively ) . ^^^ Patients with hypothyroidism showed serum concentrations of IGF 1 ( 161 . 5 + / 13 . 1 microg / L , p < 0 . 05 ) and IGFBP 3 ( 3 . 2 + / 0 . 3 microg / L , p < 0 . 05 ) significantly lower than those found in healthy volunteers . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When 20 ng / mL of IGF 1 was added to the media , IGFBP 3 was increased approximately 65 % ( P < 0 . 05 ) and IGFBP 5 was increased approximately twofold ( P < 0 . 05 ) . ^^^ Neither IGFBP 3 nor 5 was detectable in the conditioned media from fibroblasts whether or not IGF 1 , TGF beta 1 or bFGF were present . ^^^ These data suggest that the effects of IGF 1 , TGF beta 1 and bFGF on porcine satellite cells may in part be through the autocrine / paracrine production of IGFBP 3 and 5 by porcine satellite cells . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine whether preeclampsia influences insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) , independent of its effect on birth weight . ^^^ IGF 1 , IGFBP 1 , and IGFBP 3 were measured in umbilical plasma . ^^^ RESULTS : Between mild preeclampsia and controls , there were no differences in IGF 1 , IGFBP 1 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) concentrations also increased significantly while on rhGH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) can stimulate apoptosis and inhibit cell proliferation directly and independently of binding IGFs or indirectly by forming complexes with IGF 1 and IGF 2 that prevent them from activating the IGF 1 receptor to stimulate cell survival and proliferation . ^^^ To assess the general importance of IGF independent mechanisms , we have generated human IGFBP 3 mutants that can not bind IGF 1 or IGF 2 by substituting alanine for six residues in the proposed IGF binding site , Ile ( 56 ) / Tyr ( 57 ) / Arg ( 75 ) / Leu ( 77 ) / Leu ( 80 ) / Leu ( 81 ) , and expressing the 6m hIGFBP 3 mutant construct in Chinese hamster ovary cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The serum levels of IGF 1 and IGF binding protein 3 during treatment also demonstrated dependency on the GH dose and were independently correlated with the increase in height SD scores attained . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with PL , RLX reduced fibrinogen levels by 0 . 77 g / liter ( P < 0 . 001 ) , IGF 1 by 2 . 4 nmol / liter ( P < 0 . 001 ) , and free T by 0 . 73 pmol / liter ( P = 0 . 038 ) and increased SHBG by 5 . 5 nmol / liter ( P = 0 . 001 ) and IGF binding protein 3 by 0 . 57 ng / ml ( P = 0 . 007 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fifteen IUGR and 12 normal short prepubertal subjects had a 90 min frequently sampled 4 glucose tolerance test performed to measure plasma glucose , insulin , IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 1 . ^^^ In addition , 29 nonobese prepubertal subjects of normal height had fasting plasma IGF 1 and IGFBP 3 levels measured . ^^^ Nonobese subjects of normal height had higher plasma IGF 1 ( 117 + / 9 microg / liter ; P < 0 . 0001 ) and IGFBP 3 ( 2 . 34 + / 0 . 12 mg / liter ) values than the IUGR group ( P < 0 . 0005 ) . ^^^ In conclusion , short IUGR children have higher plasma IGF 1 , IGF 2 , and IGFBP 3 , when compared with normal children matched for height , weight , and pubertal status . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIMS / HYPOTHESIS : The aim of this study was to investigate the role of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 gene variants on the metabolic changes observed in response to a 100 day overfeeding protocol conducted with 12 pairs of monozygotic twins . ^^^ IGFBP 3 Nde 1 and the IGF 1 ( CT ) n markers were not associated with responsiveness to overfeeding . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and C peptide were determined . ^^^ RESULTS : Mean concentrations of plasma IGF 1 , IGFBP 1 , IGFBP 2 , IGFBP 3 , and C peptide were 156 . 1 , 14 . 3 , 434 . 4 , 3062 , and 2 . 86 ng / ml , respectively . ^^^ Women in the highest tertile for physical activity had lower plasma concentrations of IGF 1 , IGFBP 3 , and C peptide , and higher concentrations of IGFBP 1 and IGFBP 2 , as compared to women in the lowest tertile . ^^^ We did confirm that a lean body shape is associated with higher concentrations of IGFBP 1 and IGFBP 2 , and possibly also with lower concentrations of IGF 1 and IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : TBK , fat free mass ( FFM ) , moderate to vigorous intensity physical activity ( MVPA ; assessed with use of a semistructured interview ) , and serum concentrations of estradiol , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) were quantified in 51 healthy white women aged 54 76 y . ^^^ Serum estradiol , IGF 1 , and IGFBP 3 were not significant determinants of the potassium content of FFM . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Direct measurement of IGF 1 and IGFBP 3 in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis . ^^^ IGF 1 and IGFBP 3 were measured by RIA in BAL and serum . ^^^ No significant differences were observed between serum IGF 1 and IGFBP 3 from IPF patients and normal subjects . ^^^ Instead , the direct measurement in BAL revealed a significant increase of IGF 1 and IGFBP 3 in IPF patients compared to normal subjects . ^^^ BAL IGF 1 and IGFBP 3 concentrations were significantly related to inspiratory vital capacity ( IVC ) and carbon dioxide partial pressure ( PaCO 2 ) : the higher the value of IVC and the lower the value of PaCO 2 , the higher the level of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dexamethasone does not increase IGF 1 and IGFBP 3 levels in man in the absence of endogenous GH . ^^^ It has been previously shown that short term glucocorticoid administration increases circulating IGF 1 and IGFBP 3 levels both in men and rats . ^^^ Before treatment IGF 1 values were 9 . 3 3 . 6 microg / l ( mean + / SE ) and IGFBP 3 levels were 1 , 195 + / 208 microg / l . ^^^ After dexamethasone administration there were no significant changes either in IGF 1 or IGFBP 3 values ( 10 . 7 + / 4 . 1 and 1 , 110 + / 214 microg / l , respectively ) . ^^^ Our data suggest that dexamethasone is unable to increase circulating IGF 1 and IGFBP 3 levels in man in the absence of endogenous GH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MATERIAL AND METHODS : Plasma levels of IGF 1 , IGFBP 2 , and IGFBP 3 were measured preoperatively in 120 consecutive patients who underwent radical prostatectomy for clinically localized disease , postoperatively in 51 of these patients , in 44 healthy men , in 19 patients with metastases to regional lymph nodes , and in 10 patients with bone metastases . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study was planned in order to investigate the role of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and leptin , the product of the ob gene synthesized by fat tissue cells , in constitutional delay of growth and puberty ( CDGP ) which is the most frequent cause of short stature in children . ^^^ Serum IGF 1 , IGFBP 3 , insulin and plasma leptin levels were measured by immunoradiometric assay . ^^^ Mean IGF 1 and leptin levels were significantly lower in the CDGP group compared with the controls , but the mean IGFBP 3 level was not different in the two groups . ^^^ When evaluated according to pubertal status , a significant difference was found in IGF 1 , leptin and IGFBP 3 levels between prepubertal and pubertal groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In another group of 31 prepubertal children , we analized their nutritional status determining the body mass index , prealbumin , retinol binding protein , GHBP , IGF 1 , IGFBP 3 and leptin at admission . ^^^ Significant correlations were observed between IGF 1 and weight / height , IGFBP 3 and weight / height , GHBP and body mass index , and leptin and body mass index . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : The present study indicates that moderate preeclampsia is associated with low newborn birth weight , IGF 1 , IGFBP 3 , and high IGFBP 1 without significant changes in zinc and leptin levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the group of obese patients serum concentrations of free IGF 1 correlated inversely with the maximum perfusion after heat induced hyperaemia ( THmax ) ( r = 0 . 55 , p < 0 . 02 ) , and IGFBP 3 concentrations correlated inversely with maximum hyperaemia after occlusion ( PORGmax ) ( r = 0 . 57 , p < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this prospective , randomized , double blind study was to measure the concentrations of IGF 1 and IGFBP 3 in follicular fluids collected from infertile women undergoing ovarian hyperstimulation using three different gonadotropin preparations . ^^^ The concentrations of IGF 1 and IGFBP 3 in the follicular fluid of aspirated dominant follicles were measured directly . ^^^ Follicular fluid IGF 1 and IGFBP 3 concentrations did not differ significantly in the three groups of women . ^^^ A statistically significant relationship was observed between follicular fluid IGF 1 and IGFBP 3 levels ( r = 0 . 41 , p = 0 . 001 ) . ^^^ Oocyte maturation correlated in a positive manner with IGF 1 ( r = 0 . 34 , p = 0 . 01 ) and IGFBP 3 ( r = 0 . 29 , p = 0 . 03 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The relationship between ovarian structure and serum insulin , insulin like growth factor 1 ( IGF 1 ) and its binding protein ( IGFBP 1 and IGFBP 3 ) levels in premature pubarche . ^^^ The aim of this study was to determine serum insulin , insulin like growth factor 1 ( IGF 1 ) and its binding proteins ( IGFBP 1 and IGFBP 3 ) levels and their relationship with androgen levels and ovarian structure in 23 girls with premature pubarche ( PP ) . ^^^ Fasting levels of testosterone , dehydroepiandrosterone ( DHEA ) and its sulfate ( DHEAS ) , androstenedione ( delta4A ) , sex hormone binding globulin ( SHBG ) , glucose ( G ) , insulin ( 1 ) , IGF 1 , IGFBP 1 , IGFBP 3 were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before weight gain , leptin , IGF 1 , and IGFBP 3 were significantly lower and IGFBP 1 was elevated in the malnourished group compared with the control group . ^^^ Among PEM patients , after 10 % weight gains , the levels of leptin , IGF 1 , and IGFBP 3 were significantly higher and IGFBP 1 significantly lower compared with the control group . ^^^ On admission , no correlation was observed between leptin and IGF 1 ( r ( s ) = 0 . 08 , P < 0 . 16 ) and between leptin and IGFBP 3 ( r ( s ) = 0 . 02 , P < 0 . 27 ) in the malnourished group , but those levels improved after 10 % recovery of their body weights ( r ( s ) = 0 . 47 , P < 0 . 002 and r ( s ) = 0 . 42 , P < 0 . 005 , respectively ) . ^^^ In the PEM group , IGF 1 correlated significantly with IGFBP 3 when the children gained weight ( before : r ( s ) = 0 . 006 , P < 0 . 31 ; after : r ( s ) = 0 . 32 , P < 0 . 01 ) . ^^^ CONCLUSIONS : The main finding of this study was that , after refeeding with only a 10 % weight gain , the PEM children normalized their leptin , IGF 1 , and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Overall ADG was positively correlated with early stage ( d 21 ) IGF 1 and IGFBP 3 concentrations only in unimplanted barrows , whereas backfat thickness was negatively correlated with d 42 IGF 1 concentration in all but unimplanted barrows with ad libitum intake . ^^^ A strong positive correlation ( P < 0 . 01 ) between IGF 1 and IGFBP 3 concentrations was apparent with increasing age of the animals . ^^^ Results suggest that growth rate and backfat thickness are decreased by a moderate restriction of feed or energy intake with no accompanying changes in circulating IGF 1 and IGFBP 3 concentrations and that the beneficial effect of Revalor H implantation on feed efficiency may be mediated , in part , by IGF 1 . ^^^ Moreover , both IGF 1 and IGFBP 3 concentrations may be useful as growth indices in pigs . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relative to uninjected cattle , treatment with bST increased ( P < 0 . 05 ) serum concentrations of IGF 1 and IGFBP 3 and reduced ( P < 0 . 05 ) concentrations of IGFBP 2 . ^^^ Similarly , AL cattle had greater ( P < 0 . 05 ) serum concentrations of IGF 1 and IGFBP 3 and reduced ( P < 0 . 05 ) IGFBP 2 compared with 0 . 75 AL cattle . ^^^ In summary , treatment with bST increased growth rate and G : F and stimulated serum IGF 1 and IGFBP 3 while reducing IGFBP 2 . ^^^ Thus , limit feeding may reduce the effect of exogenous bST on growth rate by blunting bST induced increases in IGF 1 and IGFBP 3 and bST induced decreases in IGFBP 2 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , osteocalcin , carboxyterminal propeptide of type 1 collagen , carboxyterminal crosslink telopeptide of type 1 collagen , amino terminal propeptide of type 3 procollagen and urine pyridinolin and deoxypyridinolin were determined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The transcriptionally induced decrease in IGFBP 3 ( 20 25 % compared with control in neonates and 50 % in adult rats ) , however , seemed to be regulated by GH and IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Poor reproducibility of IGF 1 and IGF binding protein 3 generation test in children with short stature and normal coding region of the GH receptor gene . ^^^ Studies have shown that children with idiopathic short stature and GH deficiency , who were expected to be sensitive to GH , presented absent IGF 1 and IGF binding protein ( IGFBP ) 3 responses and / or discordant results at IGF 1 and IGFBP 3 generation test for unknown reasons . ^^^ Discordant responses between the first and second test were found in five and six patients for IGF 1 and IGFBP 3 , respectively . ^^^ When the results of the generation tests were compared , one notices that IGF 1 generation has more concordant positive results than IGFBP 3 . ^^^ In eight generation tests that showed discordant results between IGF 1 and IGFBP 3 , seven presented a positive IGF 1 with a negative IGFBP 3 response . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Leptin , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) were assayed by specific immunoassays . ^^^ IGF 1 and IGFBP 3 levels were significantly lower ( P < 0 . 0001 ) in homozygous GHI and GHD patients compared with either controls or GHI heterozygotes . ^^^ During the 1 wk courses of GH therapy , serum IGF 1 and IGFBP 3 levels significantly increased ( P < 0 . 0001 ) in GHD patients , heterozygous GHI patients , and control subjects at both GH doses . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present clinical study compares the impact of low and high dose parenteral testosterone ( T ) supplementation on daily GH secretory patterns and serum IGF 1 , IGFBP 1 , and IGFBP 3 concentrations in healthy older ( 60 82 yr ) and young ( 20 40 yr ) men . ^^^ To monitor somatotropic axis responses , blood was sampled every 10 min for 24 h for later chemiluminescence based assay of serum GH , RIA of serum IGF 1 , and immunoradiometric assay of serum IGFBP 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For this purpose , the effects of exogenous pGH ( 1 10 , 000 ng / ml ) , PKA blockers KT 5720 ( 100 ng / ml ) and Rp cAMPS ( 1micromol ) , alone and in combination , on insulin like growth factor type 1 ( IGF 1 ) , insulin like binding protein 3 ( IGFBP 3 ) , oxytocin ( OT ) and prostaglandin F alpha ( PGF ) secretion , PKA and cAMP response element binding transcription factor ( CREB ) content and the occurrence of apoptosis were investigated . ^^^ It was found ( using RIA / IRMA ) that GH addition to culture medium significantly stimulated IGF 1 and PGF release and inhibited IGFBP 3 and OT secretion . ^^^ Furthermore , PKA blockers were able to prevent stimulatory effects of GH on IGF 1 and PGF release , and inhibitory effect of GH on IGFBP 3 , OT output and on apoptosis . ^^^ These observations suggest the involvement of GH and a PKA dependent intracellular mechanism in the control of IGF 1 , IGFBP 3 , OT , PGF , cAMP and apoptosis in porcine ovarian granulosa cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Diagnostic value of serum IGF 1 and IGFBP 3 in growth hormone disorders in adults . ^^^ OBJECTIVE : To investigate the diagnostic value of serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) measurements in adult patients with acromegaly and GH deficiency ( GHD ) . ^^^ METHODS : Serum IGF 1 and IGFBP 3 levels were measured in 39 active acromegalic patients , 34 adult patients with GHD and 150 healthy adults . ^^^ To compare the individual serum IGF 1 and IGFBP 3 levels of patients with the results of the gold standard , we calculated age and sex corrected standard deviation scores ( SDS ) for individual IGF 1 and IGFBP 3 levels . ^^^ The sensitivities of serum IGF 1 and IGFBP 3 measurements for the disease diagnosis were analyzed using the mean + / 2 SD of the values of healthy control subjects as a diagnostic cutoff , defining 95 % specificity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In all children serum concentration of IGF 1 was estimated by radioimmunoassay and the third fraction of IGF binding proteins ( IGFBP 3 ) by immunoradiometry . ^^^ There was no significant correlation between the concentration of IGF 1 and IGFBP 3 and other studied bone metabolism markers . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In vivo binding of ( 125 ) 1 IGF 1 in AF correlated highly with AF IGF binding protein 3 concentrations ( r ( 2 ) = 0 . 93 , p < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Intact PTH , osteocalcin , 1 , 25 ( OH ) ( 2 ) D ( 3 ) , 25 OH D ( 3 ) , IGF 1 , procollagen type 1 carboxy terminal extension peptide ( PICP ) and procollagen type 1 cross linked carboxy terminal telopeptide ( ICTP ) were studied by RIA , whereas IGFBP 1 and IGFBP 3 concentrations were assayed by IRMA . ^^^ We found a significantly higher IGF 1 and IGFBP 3 in rHuEPO treated HD patients when compared to CAPD subjects given rHuEPO as well as to hemodialysis ( HD ) patients not treated with rHuEPO . ^^^ In rHuEPO treated CAPD subjects IGF 1 and IGFBP 1 correlated positively with serum CrossLaps ( r = 0 . 61 , p < 0 . 05 and r = 0 . 64 , p < 0 . 05 , respectively ) , whereas in hemodialyzed patients without rHuEPO a significant negative correlation between IGFBP 3 and serum CrossLaps was found ( r = 0 . 69 , p < 0 . 001 ) as well as between IGFBP 3 and aluminium ( r = 0 . 51 , p < 0 . 05 ) , IGF 1 and ICTP ( r = 0 . 43 , p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess the relationships among circulating insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , prostate specific antigen ( PSA ) and C reactive protein ( an indicator of inflammatory systemic response ) in patients with prostate disease and cancer of different stages . ^^^ PATIENTS AND METHODS : Circulating IGF 1 , IGFBP 3 , PSA and C reactive protein concentrations were measured in patients with BPH ( 17 ) or stages T1 / T2 ( 15 ) , T3 / T4 ( 16 ) and metastatic prostate cancer ( 12 patients ) . ^^^ RESULTS : IGF 1 and IGFBP 3 concentrations were similar between patients with BPH and those with cancer , and there was no difference between the groups with cancer . ^^^ In the latter patients there was a significant correlation between age and IGFBP 3 concentrations ( r = 0 . 400 , P = 0 . 008 ) but not with IGF 1 concentrations . ^^^ Controlling for age , there were significant partial correlations between C reactive protein and IGF 1 ( r = 0 . 412 , P = 0 . 008 ) and IGFBP 3 ( r = 0 . 277 , P = 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Secondary measures included insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels , physical activity index , a 7 day dietary recall , tobacco product use , and measurement of the intima media thickness ( IMT ) of the common carotid artery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To examine the relationship between insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and body mass index and prostate volume , as a surrogate marker for benign prostatic hyperplasia , in a community based sample of black men . ^^^ Epidemiologic studies examining the role of IGF 1 and IGFBP 3 suggest that increased levels of serum IGF 1 and decreased levels of serum IGFBP 3 are associated with an increased risk of prostate cancer . ^^^ All subjects completed a clinical examination , which included a complete urologic examination with transrectal ultrasonography , anthropometric measurements , and serum assays for IGF 1 and IGFBP 3 . ^^^ IGFBP 3 rather than IGF 1 was positively associated with increasing prostate volume ( P = 0 . 003 ) . ^^^ CONCLUSIONS : This is the largest study describing the relationships between IGF 1 , IGFBP 3 , and body mass index and prostate volume , and the only study in black men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , cDNA microarray analysis was used to screen for genes : 1 ) that were regulated by IGFBP 3 in early but not in terminally differentiated chondrocytes ; 2 ) that were regulated specifically by IGFBP 3 , but not by IGF 1 ; and 3 ) whose regulation was abolished by coincubation of IGFBP 3 with IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There were minimal effects of hypoxia and GH therapy on plasma insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , and hepatic IGF 1 mRNA expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These patients had low IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and GH binding protein ( GHBP ) , but a normal or exaggerated GH response to provocative stimuli , suggestive of partial GH insensitivity ( GHI ) . ^^^ Basal blood samples were taken for IGF 1 , IGFBP 3 and GHBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentrations of 17beta oestradiol , IGF 1 and IGF binding protein 3 increased during testosterone plus placebo treatment , but during testosterone plus letrozole treatment the concentrations remained unchanged . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , TNF alpha markedly decreased IGF 1 mRNA ( 85 % reduction at 24 hours ) and increased IGFBP 3 mRNA and protein ( 300 % increase at 24 hours ) . ^^^ CONCLUSIONS : TNF alpha , a cytokine that is upregulated in atherosclerotic plaques , reduces IGF 1 and increases IGFBP 3 in VSMCs , likely leading to a reduction in bioactive IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and insulin like growth factor binding protein 3 correlated ( P < or = 0 . 05 ) with all measures of BMC ( r values ranged from 0 . 31 to 0 . 39 ) and some measures of lean soft tissue mass for women ( r = 0 . 30 ) but not men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 response to growth hormone is impaired in HIV infected children . ^^^ To better characterize the somatotropic axis in HIV infected children the circadian rhythm of growth hormone ( GH ) , and basal and stimulated ( by an insulin like growth factor 1 [ IGF 1 ] generation test ) plasma levels of IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) , were evaluated in 16 children ( 9 boys and 7 girls ; age range , 7 11 years ) with HIV infection . ^^^ As regards the IGF 1 generation test , biosynthetic human GH ( hGH , 0 . 1 IU / kg , 0 . 033 mg / kg ) was administered subcutaneously for 4 days and blood samples were taken from fasting subjects at baseline and on the morning after the last GH injection for measurement of IGF 1 and IGFBP 3 . ^^^ It appears that circadian GH secretion is normal in children with HIV infection , but the response to exogenous GH with regard to IGF 1 and IGFBP 3 production is impaired , indicating a degree of GH insensitivity in such children . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The serum GH concentration was significantly decreased during the first 2 months in knockout female and male mice , and IGF 1 and IGF binding protein 3 levels were lower in knockout mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS AND RESULTS : Determination of types 1 and 2 acute phase reactant proteins , constitutive serum proteins , serum cytokines , serum IGF 1 , IGFBP 3 , and growth hormone levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels were measured in a subset of 293 subjects in the adenoma study ( 135 case patients and 158 control subjects ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , functional studies showed that nitric oxide donors elicited a stimulatory action on production of IGF 1 in corpora lutea from the early luteal phase ( 80 % ) and on production of IGFBP 1 in corpora lutea from the late luteal phase ( 50 % ) ( P < 0 . 05 ) , whereas production of IGF 2 and IGFBP 3 was not affected by nitric oxide . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BMD in total body , distal and UD radius , and femoral neck was positively correlated with serum IGF 1 ( r = 0 . 31 to 0 . 49 ) , IGF 2 ( r = 0 . 32 to 0 . 48 ) , IGFBP 3 ( r = 0 . 37 to 0 . 53 ) , and free androgen index ( FAI ) ( r = 0 . 32 to 0 . 40 ) , and negatively with IGFBP 1 ( r = 0 . 37 to 0 . 41 ) and IGFBP 2 ( r = 0 . 29 to 0 . 41 ) levels . ^^^ Age correlated negatively with serum IGF 1 , IGF 2 , IGFBP 3 , FAI , estradiol / SHBG ratio , and BMD in total body , distal and UD radius , and femoral neck , and positively with IGFBP 1 , IGFBP 2 , and SHBG levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To determine whether these racial differences occur in prepubertal boys , we measured spontaneous overnight GH secretion , serum testosterone , 17 beta estradiol , IGF 1 , and IGFBP 3 , IGF I / IGFBP 3 ratio , BMD of the total body , forearm , lumbar spine , trochanter , and femoral neck , and lean body mass and body fat in 14 healthy black and 16 white boys ages 6 7 years . ^^^ Whereas serum testosterone , serum 17 beta estradiol , and serum IGF 1 were not different , serum IGFBP 3 was higher and the molar ratio of serum IGF l / IGFBP 3 was lower in white than in black males . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This increase of bone mineral density was not accompanied by changes in insulin like growth factor 1 ( IGF 1 ) or insulin like growth factor binding protein 3 ( IGFBP 3 ) in either group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Reduced serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in adults with inflammatory bowel disease . ^^^ In the present study , the changes in circulating IGF 1 and its binding protein IGFBP 3 were determined in adult patients with active inflammatory bowel disease ( IBD ) in order to assess the effect of this inflammatory condition on the IGF system . ^^^ IGF 1 and IGFBP 3 , as well as interleukin 6 ( IL 6 ) were measured in serum obtained from 22 consecutive newly diagnosed patients ( mean age 41 . 3 years ) with active IBD , including 10 patients with Crohn ' s disease ( CD ) , and 12 with ulcerative colitis ( UC ) . ^^^ Serum IGF 1 and IGFBP 3 levels were similar in the two subgroups of patients and the values from all patients were combined for comparison with those from the control group . ^^^ The reduced IGF 1 and IGFBP 3 levels in patients with active IBD suggest that this systemic inflammatory condition is associated with a degree of acquired GH resistance , possibly induced by inflammatory cytokines . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The rhGH administration led to significant increases of IGF 1 , total IGFBP 3 , glycosylated IGFBP 3 ( 39 , 42 kDa ) and the 29 kDa fragment of IGFBP 3 and the decrease of IGFBP 2 during the trial ( P < 0 . 05 ) . ^^^ In spite of the significant changes of IGF 1 , IGFBP 2 , total IGFBP 3 and IGFBP 3 ( 29 kDa proteolytic fragment ) after rhGH administration to patients with malnutrition , maintained on parenteral nutrition , the increase of nitrogen balance was seen only in patients who decreased their IGFBP 1 and increased bioavailable IGF 1 as reflected by measurement of fIGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Clinical data were recorded at the time of bone density measurement , concomitant with blood sample extraction for study of IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 1 , estradiol , sex hormone binding globulin , dehydroepiandrosterone sulfate , prealbumin , amino terminal propeptide of procollagen 3 , osteocalcin , bone alkaline phosphatase , carboxy terminal propeptide of procollagen 1 , amino terminal propeptide of procollagen 1 , carboxy terminal telopeptide of collagen 1 , 25 OH vitamin D , 1 , 25 ( OH ) ( 2 ) vitamin D , and parathormone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This increase in concentrations of intact IGFBP 3 and IGFBP 5 leads to an increase in IGF 1 which is associated with an improvement in joint architecture during the development of OA . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the current study , growth inhibition by IGF 1 and IGF 2 was blocked by concurrent addition of IGFBP 3 , implying that colon cancers with elevated IGFBP 3 levels would be selected for in vivo because they could bind and inactivate high serum IGF 1 levels and continue to proliferate . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Low free and total insulinlike growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in chronic inflammatory bowel disease : partial normalization during prednisolone treatment . ^^^ METHODS : The aim of the present study was to examine changes in total and free IGF 1 , IGF binding proteins ( IGFBPs ) , and IGFBP 3 protease activity in 10 patients with severe acute activity in colon CIBD before and during high dose prednisolone treatment ( 1 wk ) and 3 months tapering . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 levels in pigs fed the LL diet were 52 % lower than in those fed the control diet ( P < 0 . 01 ) , and the LL group also had lower plasma IGF binding protein 3 ( IGFBP 3 ) levels ( P < 0 . 05 ) . ^^^ The lower plasma IGFBP 3 in pigs fed the LL diet suggests that increased clearance rates of circulating IGF 1 may have been involved in this response . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Role of the steroids , SHBG , IGF 1 , IGF BP 3 and growth hormone in glucose metabolism disorders during long term treatment with low doses of glucocorticoids ] . ^^^ BACKGROUND : The relationships between selected steroids , SHBG , growth hormone , IGF 1 , IGF BP 3 and indicators of glucose metabolism were studied in the group of 20 female patients ( 15 20 yrs ) on long term treatment with low prednisone doses ( < 0 . 3 mg / kg / day ) ( baseline phase ) and after adding 1000 mg of metformin per day for following 6 months to improve impaired glucose metabolism ( control phase ) . ^^^ The tendency of GH to lower , and IGF 1 , IGF BP 3 to higher values at the baseline changed at the control phase : fasting and AUC value of GH increased ( signif . ) , while were IGF 1 ( nonsignif . ) and IGF BP 3 ( signif . ) levels decreased . ^^^ The same relationships were found between AUC IGF BP 3 ( +0 . 5676 , +0 . 5559 ) , IGF 1 ( HOMAIR only +0 . 5412 ) , IGF 1 / IGF BP 3 ( +0 . 5059 , +0 . 5716 ) and parameters of insulin sensitivity ( HOMAIR , insulin / glucose ) in the control period . ^^^ For the first time negative correlations between IGF 1 , IGF 1 AUC , IGF BP 3 , IGF 1 / IGF BP 3 and somatostatin blood levels were discovered at the control phase . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The main objective of this study was to evaluate whether nighttime upper airway obstruction attributable to adenotonsillar hypertrophy and subsequent surgical treatment affect the circulating concentrations of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) along with other growth parameters in children . ^^^ Data on anthropometry and circulating concentrations of IGF 1 and IGFBP 3 were generated and compared with corresponding characteristics in control children ( N = 35 ) . ^^^ No differences were observed in peripheral IGF 1 concentrations , but both OSAS and PS children had reduced peripheral IGFBP 3 levels . ^^^ Both the IGF 1 and the IGFBP 3 concentrations increased significantly in the operated children , whereas no significant changes were seen in the PS children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To this goal , in 22 patients with burn [ BURN , age ( mean+ / SE ) : 46 . 5+ / 3 . 4 yr , BMI : 25 . 0+ / 0 . 8 kg / m2 , % burn surface area : 26 . 0+ / 3 . 0 % , ROI score : 0 . 22+ / 0 . 1 ] we evaluated IGF 1 , IGF binding protein ( IGFBP 3 ) , GH , GH binding protein ( GHBP ) , pre albumin ( pre A ) , albumin ( A ) and transferrin ( TRA ) levels on days 1 , 3 , 7 , 14 and 28 after intensive care unit ( ICU ) admission . ^^^ IGF 1 , IGFBP 3 , GH and GHBP levels were also assayed basally in 29 normal subjects ( Ns ) ( Ns , age : 47 . 5+ / 2 . 8 yr , BMI : 22 . 0+ / 1 . 4 kg / m2 ) and in 34 panhypopituitary patients with severe GH deficiency ( GHD , age : 42 . 7+ / 2 . 5 yr , BMI : 25 . 6+ / 0 . 8 kg / m2 ) . ^^^ On ICU day 1 , IGF 1 and IGFBP 3 in BURN were higher than those in GHD ( p < 0 . 05 for both , respectively ) and lower than those in Ns ( p < 0 . 05 ) while GH levels in BURN did not differ from those in Ns and higher than GHD ( p < 0 . 01 ) . ^^^ In BURN , IGF 1 and IGFBP 3 levels showed a progressive decline ( p < 0 . 05 ) with nadir on day 14 , when they overlapped those in GHD , and then an increase on day 28 , though persisting lower than in Ns , while GH levels did not vary during ICU stay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Breast carcinoma derived cells ( MCF 7 ) were stimulated by des ( 1 3 ) IGF 1 or [ Gln ( 3 ) , Ala ( 4 ) , Tyr ( 15 ) , Leu ( 16 ) ] IGF 1 , two IGF analogues with intact affinity for IGF IR , but with weak or virtually no affinity for IGFBPs , then incubated with IGFBP 3 . ^^^ Several cell lines derived from normal or tumor cells responsive to IGF 1 were used to show that IGFBP 3 stimulated PTPase is cell type specific . ^^^ Although the precise nature of the phosphatase remains to be determined , the results of this study demonstrate that IGFBP 3 stimulates a phosphotyrosine phosphatase activity that down regulates the IGF 1 signaling pathway , suggesting a major role for IGFBP 3 in regulating cell proliferation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Hypertrophy induced by IGF 1 was blocked by IGF binding protein 3 , which binds IGF 1 , while that induced by IGF 2 was blocked by antibodies against IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To explore the effect of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) on the fetal growth . ^^^ Serum IGF 1 , IGF 2 and IGFBP 3 were measured by radioimmunoassay ( RIA ) and immunoradiometric analysis ( IRMA ) . ^^^ The fetal IGF 1 , IGF 2 and IGFBP 3 levels in FGR group were significantly lower than those in control group ( P < 0 . 01 ) . ( 5 ) The levels of fetal IGF 1 , IGF 2 , IGFBP 3 were positively correlated with birth weight ( r = 0 . 61 , r = 0 . 51 and 0 . 63 , P < 0 . 01 ) and placental weight ( r = 0 . 47 , r = 0 . 56 and 0 . 48 , P < 0 . 01 ) . ^^^ The levels of fetal IGF 1 , IGF 2 and IGFBP 3 were no different between macrosomia group and control group . ^^^ CONCLUSIONS : This study suggest that ( 1 ) IGF 1 , IGF 2 and IGFBP 3 can not pass through the placenta . ( 2 ) The betal IGF 1 , IGF 2 and IGFBP 3 may have the close relationship with the fetal growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To study the changes of plasma insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) in patients with acute cerebral infarct ( ACI ) and acute cerebral hemorrhage ( ACH ) and the correlation of IGF 1 and IGFBP 3 with plasma fasting insulin ( FINS ) related to insulin resistance ( IR ) and with insulin sensitive index ( ISI ) . ^^^ METHODS : The plasma IGF 1 , IGFBP 3 and FINS of 34 patients with ACI and 40 patients with ACH ( within 2 days after onset ) were measured by radioimmunoassay . ^^^ CONCLUSION : The plasma IGF 1 and IGFBP 3 participates in the pathophysiologic course of ACI and ACH in the early stage . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , the pubertal growth spurt in a number of morphological characteristics of female rhesus monkeys is related to serum endocrine status of insulin like growth factor 1 ( IGF 1 ) and its binding protein , insulin like growth factor binding protein 3 ( IGFBP 3 ) , to test the hypothesis that elevations in IGF 1 and IGFBP 3 coincide with the time of greatest growth rate of different morphological characteristics . ^^^ Elevations in serum IGF 1 and IGFBP 3 took place in a manner similar to that of humans , and across the period associated with onset of puberty . ^^^ Significant elevations in IGF 1 and IGFBP 3 were not coincident with greatest gains in foot length or crown rump length . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Impaired blockade of insulin like growth factor 1 ( IGF 1 ) induced hypoglycemia by IGF binding protein 3 analog with reduced ternary complex forming ability . ^^^ In this study , we show that hypoglycemia , induced by a bolus injection of recombinant human IGF 1 into rats , can be blocked by coadministering equimolar concentrations of either recombinant glycosylated IGFBP 3 or nonglycosylated IGFBP 3 ( IGFBP 3NG ) . ^^^ In contrast , an IGFBP 3 mutant with reduced acid labile subunit affinity ( IGFBP 3MUT ) only partially blocked the IGF 1 hypoglycemic effect . ^^^ IGFBP 3 and IGFBP 3NG significantly enhanced IGF 1 retention in the circulation , whereas IGFBP 3MUT had a smaller effect . ^^^ IGFBP 3MUT clearance was more rapid than that of the other IGFBP 3 forms , and the retention of all IGFBP 3 forms was greatly enhanced by coadministration of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured the binding of IGF 1 and IGF 2 to recombinant human N terminal [ residues 1 97 ; recombinant human IGF binding protein 3 ( 1 97 ) ( rhIGFBP 3 ( 1 97 ) ) ] and C terminal ( residues 98 264 ; rhIGFBP 3 ( 98 264 ) ) IGFBP 3 fragments and compared it with IGF binding to intact IGFBP 3 using biosensor analysis . ^^^ These experiments showed that IGF 1 and IGF 2 bind to IGFBP 3 with affinities of 4 5 10 10 ( 9 ) M ( 1 ) and similar binding kinetics . ^^^ Binding of insulin to IGFBP 3 and its N and C terminal fragments and of IGF 1 and IGF 2 to the structurally related proteins mac 25 and connective tissue growth factor was also investigated . ^^^ Weak insulin binding to full length IGFBP 3 could be demonstrated in a few experiments , but we found that binding of IGF 1 , IGF 2 , and insulin to mac 25 or connective tissue growth factor was below the detection limit of the biosensor instrument . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Skin fibroblasts of children with idiopathic short stature show an increased mitogenic response to IGF 1 and secrete more IGFBP 3 . ^^^ IGFBP 3 secretion was increased in ISS fibroblasts when compared to controls under all conditions examined ( basal , 200 and 5000 ng / ml GH , 10 ng / ml IGF 1 for 24 and 48 h ) . ^^^ High IGFBP 3 levels were related to low mitogenic responses to IGF 1 or to GH + IGF 1 in children with ISS ( r = 0 . 7 , P < 0 . 05 ) , but not in controls . ^^^ CONCLUSION : The demonstration of high IGFBP 3 levels and enhanced mitogenic response to IGF 1 shows that ISS fibroblasts have different cellular characteristics compared to controls of normal height . ^^^ It is hypothesized that in ISS an alteration of the signal transduction pathway between the GH receptor and IGFBP 3 synthesis results in a local imbalance with high IGFBP 3 levels and lower IGF 1 availability for the IGF 1 receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 concentrations were determined at a central laboratory and converted to SDS values by reference to a normal population . ^^^ Only the group classed as GH deficient by both criteria had a mean IGFBP 3 less than 2 SDS and both IGF 1 SDS and IGFBP 3 SDS increased steadily across the four groups . ^^^ When GH stimulation test results and IGF 1 concentration are discordant , the IGFBP 3 level does not establish diagnosis and the hGH treatment requirement of such patients remains a dilemma . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We speculate that some of the differences between clones and controls in concentrations of GH , IGF 1 , and IGFBP 3 may be related to the genetic merit of the animals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The major binding protein for IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , modulates the effects of IGF 1 but also inhibits cell growth and induces apoptosis independent of IGF 1 and its receptor . ^^^ In a prospective study of men in Shanghai , China , we examined the association between serum levels of IGF 1 and IGFBP 3 and the subsequent risk of lung cancer . ^^^ We analyzed IGF 1 and IGFBP 3 levels in serum from 230 case patients who developed incident lung cancer during follow up and from 740 control subjects . ^^^ However , for subjects in the highest quartile relative to the lowest quartile of IGFBP 3 , the odds ratio ( OR ) for lung cancer , adjusted for smoking and IGF 1 , was 0 . 50 ( 95 % confidence interval [ CI ] = 0 . 25 to 1 . 02 ) . ^^^ When the analysis was restricted to ever smokers ( 184 case patients and 344 matched control subjects ) , the OR for lung cancer in men in the highest quartile of IGFBP 3 relative to those in the lowest quartile , adjusted for smoking and IGF 1 , was 0 . 41 ( 95 % CI = 0 . 18 to 0 . 92 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Northern blot analysis showed an up regulation of IGF binding protein 3 mRNA levels , which suggests that the protein deprivation may lead to an increased sequestration of circulating or locally synthesized IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Longer incubations ( 1 4 h ) with FSH alone resulted in the elevation of AKT phosphorylation concomitant with an increased secretion of IGF 1 and decreased production of IGF binding protein 3 , implicating endogenous IGF 1 in the action of FSH on AKT phosphorylation . ^^^ IGF 1 and FSH dependent AKT phosphorylation was inhibited by LY 29400 ( 10 microM ) , a PI3K inhibitor , and by IGF binding protein 3 , but not by a PKA inhibitor ( H 89 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 were measured at rest . ^^^ Currently , we report that RT had no effect on the resting serum concentrations of IGF 1 , IGFBP 1 , IGFBP 3 , testosterone , or cortisol . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and the corresponding binding protein ( IGFBP 3 ) , markers of bone metabolism and lumbar and femoral neck BMD were measured in 28 adult patients , undergoing hormonal replacement and chelation therapy and a hypertransfusion program , with beta thalassaemia major ( 12 males with mean age 22 . 5+ / 3 . 1 and 16 females with mean age 27 . 5+ / 8 . 2 ) , and in 28 healthy volunteers matched for age , anthropometric features and sex to the patients . ^^^ Our beta thalassaemic patients , in spite of normalizing hemoglobin levels , adequate hormone replacement and chelation therapies , showed osteopenia and an unbalanced bone turnover with an increased resorptive phase and a decreased formation phase probably correlated to low levels of IGF 1 and IGFBP 3 observed in our study . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Short term infusion of LongR ( 3 ) insulin like growth factor ( IGF ) 1 decreases hepatic IGF 1 mRNA but not IGF binding protein 3 mRNA expression in pigs . ^^^ Infusion of pigs with an insulin like growth factor 1 ( IGF 1 ) analogue ( LongR ( 3 ) IGF 1 ) that does not bind to IGF binding proteins decreases growth rate and the plasma concentration of growth hormone ( GH ) , IGF 1 , IGFBP 3 , and insulin . ^^^ This study was designed to determine whether the decrease is due to changes in IGF 1 and IGFBP 3 gene expression . ^^^ Plasma IGF 1 concentration was measured by radioimmunoassay and plasma IGFBP 3 and IGFBP 2 were estimated by Western ligand blotting . ^^^ Steady state levels of IGF 1 and IGFBP 3 mRNA were measured by RNase protection assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Patients revealed suppressed pulsatile GH , TSH and LH secretion in the face of low serum concentrations of IGF 1 , IGFBP 3 and the acid labile subunit ( ALS ) ( P < 0 . 0001 each ) , thyroid hormones ( P < 0 . 0001 ) and total and estimated free testosterone ( P < 0 . 0001 ) levels , whereas free oestradiol ( E 2 ) estimates were normal . ^^^ GHRP 2 infusion reactivated GH secretion and normalized serum IGF 1 , IGFBP 3 and ALS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Umbilical cord blood was collected in 12 804 consecutive deliveries , and cord plasma from 585 singleton infants born at term after uncomplicated pregnancies was analyzed for leptin , IGF 1 , and 2 IGF binding proteins ( IGFBP 1 and IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with rhGH increased serum IGF 1 ( median increase over placebo , +93 microg . liter ( 1 ) ; P = 0 . 004 ) , IGF binding protein 3 ( +0 . 9 mg . liter ( 1 ) : P = 0 . 004 ) , and acid labile subunit ( +10 . 7 nM ; P = 0 . 004 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were obtained before insulin injection and breakfast to test for plasma glucose ( PG ) , IGF 1 , IGFBP 1 , IGFBP 3 , total and complex GHBP ( tGHBP and cGHBP ) , and HbA1c . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Such a powerful increase was correlated by a small enhancement in insulin like growth factor binding protein 3 ( IGFBP 3 ) plasma levels , while treatment with IGF 1 alone affected very significantly both IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Less IGFBP 3 proteolysis was associated with more weight loss , suggesting that this could be a protective counter regulatory mechanism , increasing IGF 1 bioavailability to the tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Globally , risk was unassociated with levels of IGF 1 , IGFBP 3 , or IGF 1 adjusted for IGFBP 3 . ^^^ Levels of IGF 1 and IGFBP 3 were not related to risk in younger women , recruited before age 50 , contrary to observations from previous studies . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 level had a positive correlation with IGFBP 3 ( R=0 . 56 , p=0 . 01 ) and free IGF 1 ( R=0 . 53 , p=0 . 01 ) . ^^^ The authors found no correlations between IGF 1 , IGFBP 3 , free IGF 1 , IGFBP 1 and HbA1c in the study . ^^^ It was , therefore , postulated that a near normal free IGF 1 level in diabetic children resulted from a balance of interaction between IGFBP 1 and IGFBP 3 to total IGF 1 in order to keep the normal metabolic status as much as possible . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously shown that TGF beta 1 increased IGF 1 and IGF binding protein ( IGFBP ) 3 production in human bone marrow stromal ( hMS ) osteoblast progenitors and calcitriol stimulated IGFBP 3 and IGFBP 4 production . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Despite increasing clinical use and recent evidence that insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and human growth hormone ( hGH ) target the heart , the clinical manifestations following the change in the serum growth factors in infants with isolated ventricular septal defect ( VSD ) undergoing surgical repair have not been clearly defined . ^^^ Serum IGF 1 , IGFBP 3 , and hGH factors were determined by enzyme linked immunosorbent assay using a monoclonal antibody . ^^^ In comparison to group 1 , the decrease in serum levels of IGF 1 and IGFBP 3 in groups 2 and IIIa were statistically significant ( in group 2 43 % and 32 % , p < 0 . 05 ; in group IIIa 79 % and 37 % , p < 0 . 01 ) . ^^^ Interestingly , the change in serum levels of IGF 1 , IGFBP 3 ( decrease ) , and hGH ( increase ) , returned to the normal range of serum levels after VSD repair in group IIIb . ^^^ CONCLUSIONS : Improvement in serum levels of IGF 1 , IGFBP 3 , and hGH were identified in infants with VSD after surgical repair . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline determinations of total IGF 1 , free IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) were obtained in 19 patients before starting ERT at a mean age of 13 . 8 + / 11 . 2 y . ^^^ Total IGF 1 , free IGF 1 , and IGFBP 3 were expressed as SD scores ( SDS ) . ^^^ Total IGF 1 and IGFBP 3 SDS correlated negatively with the presence of the L444P mutation ( r = 0 . 53 and 0 . 5 , respectively , p < 0 . 05 ) . ^^^ Height SDS correlated with total IGF 1 and IGFBP 3 SDS in eight children ( r = 0 . 84 and 0 . 78 , respectively , p < 0 . 05 ) . ^^^ Total IGF 1 and IGFBP 3 are the two parameters that better correlate with height before treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : We investigated whether supplementation with 60 g / d of bovine colostrum affects blood levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in relation to doping testing . ^^^ CONCLUSIONS : Daily supplementation with 60 g of bovine colostrum for 4 wk does not change blood IGF 1 or IGF binding protein 3 levels and does not elicit positive results on drug tests . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Birth weight ( BW ) , birth length ( BL ) , ponderal index , head circumference ( HC ) , and cord serum levels of albumin , prealbumin , retinol binding protein ( RBP ) , total and free IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and leptin were significantly lower , whereas levels of IGFBP 1 , IGFBP 2 , and IL 6 were significantly higher in preterm than in term neonates ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , comparison of both groups revealed a significantly higher increase in TGF beta 1 , IGF 1 , IGFBP 3 , and hGH in the lengthening group during the distraction period , indicating key regulatory functions in mechanotransduction . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGFBP 3 and acid labile subunit were measured and the dependency of these factors on covariates such as log 10 mean serum GH , sex , age and prior radiotherapy was assessed using regression techniques . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 155 healthy boys ( 12 . 0 + / 1 . 5 years ; range 8 . 8 15 . 7 years ) and 151 healthy girls ( 11 . 2 + / 1 . 6 years ; range 8 . 2 14 . 0 years ) markers of bone formation and bone resorption were measured as well as sex steroids , IGF 1 and IGF BP 3 , together with bone mineral content ( BMC ) and bone mineral density ( BMD ) of the lumbar spine , femur and total body during puberty . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) is an important growth factor for breast cancer cells and insulin like growth factor binding protein 3 ( IGFBP 3 ) its most prevalent binding protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the GH / IGF 1 axis and the levels of IGF binding proteins ( IGFBPs ) , IGFBP 3 protease , glucose , insulin , and cytokines in 27 children with severe septic shock due to meningococcal sepsis during the first 3 d after admission . ^^^ Significant differences were found between nonsurvivors and survivors for the levels of total IGF 1 ( 2 . 6 vs . 5 . 6 nmol / liter ) , free IGF 1 ( 0 . 003 vs . 0 . 012 nmol / liter ) , IGFBP 1 ( 44 . 3 vs . 8 . 9 nmol / liter ) , IGFBP 3 protease activity ( 61 vs . 32 % ) , IL 6 ( 1200 vs . 50 ng / ml ) , and TNFalpha ( 34 vs . 5 . 3 pg / ml ; P < 0 . 01 ) . ^^^ The pediatric risk of mortality score correlated significantly with levels of IGFBP 1 , IGFBP 3 protease activity , IL 6 , and TNFalpha ( r = +0 . 45 to +0 . 69 ) and with levels of total IGF 1 and free IGF 1 ( r = 0 . 44 and 0 . 55 , respectively ) . ^^^ Follow up after 48 h in survivors showed an increased number of GH peaks , increased free IGF 1 and IGFBP 3 levels , and lower IGFBP 1 levels compared with admission values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated these results in conjunction with IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels . ^^^ Nadir GH levels after the ingestion of 75 g of glucose , as well as baseline IGF 1 and IGFBP 3 levels , were evaluated in 56 normal adult subjects and 32 previously treated acromegalic patients . ^^^ Their mean basal IGF 1 and IGFBP 3 levels were 160 + / 58 microg / liter and 1926 + / 497 microg / liter , respectively . ^^^ Acromegalic patients had mean GH nadir , IGF 1 , and IGFBP 3 levels higher than those of normal subjects ( 2 . 6 + / 7 . 6 microg / liter , 313 + / 246 microg / liter , and 2625 + / 1154 microg / liter , respectively ) . ^^^ IGF 1 and IGFBP 3 levels were normal in 16 and 15 of these 19 patients , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition , IGF 1 and IGFBP 3 concentrations as outcome measures in severely GH deficient ( GHD ) patients after childhood GH treatment : a comparison with adult onset GHD patients . ^^^ Standard deviation scores for IGF 1 ( CO female , 9 . 2 + / 3 . 1 ; AO female , 5 . 2 + / 2 . 6 ; CO male , 6 . 4 + / 2 . 7 ; AO male , 3 . 5 + / 2 . 3 ; P < 0 . 001 within each gender ) and IGFBP 3 ( CO female , 3 . 5 + / 2 . 5 ; AO female , 1 . 7 + / 1 . 5 ; CO male , 2 . 8 + / 2 . 0 ; AO male , 1 . 1 + / 1 . 6 ; P < 0 . 001 within each gender ) were significantly lower in the CO group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
They underwent clinical examination and assessment of plasma or serum levels of ghrelin leptin , soluble leptin receptor , IGF 1 , IGFBP 1 and IGFBP 3 . ^^^ Furthermore , decreased serum levels of IGF 1 ( p < 0 . 01 ) and IGFBP 3 ( p < 0 . 001 ) were found in patients with short bowel syndrome . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
On ICU day 1 , IGF 1 and 2 and IGFBP 3 in SEP were lower ( p < 0 . 05 ) than in TRA which , in turn , were lower ( p < 0 . 01 ) than in AS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Plasma levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 were determined from the fasting bloods of 206 BPH cases admitted for treatment and 306 randomly selected population controls in Shanghai , China . ^^^ CONCLUSION : As has been previously observed for prostate cancer , we found that IGF 1 and IGFBP 3 are associated with BPH risk in China . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A period of 20 min of H / I that resulted in histopathology in cortex , striatum , and thalamus was correlated with induction of mRNA for IGF 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and glial fibrillary acidic protein ( GFAP ) by 4 days of recovery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , vitamin D , and intact parathyroid hormone ( iPTH ) were measured . ^^^ There was a significant increase in the IGF 1 and a significant reduction in the IGFBP 3 concentrations at 6 months post RTx ( p < 0 . 05 ) . ^^^ The circulating levels of IGF 1 and IGFBP 3 stimulated by the reduction in BMD and IGF 1 secretion are increased in order to restore bone formation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the brain regions and posterior pituitary , IGFBP 3 was easily washed away during pre incubation procedures as used in the [ ( 125 ) 1 ] IGF 1 binding experiments . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma insulin like growth factor ( IGF ) 1 , free IGF 1 and 2 , IGF binding protein ( IGFBP ) 1 , 2 , and 3 together with IGFBP 3 protease activity were measured in 114 postmenopausal and 39 premenopausal healthy women . ^^^ Postmenopausal women had lower levels of plasma IGF 1 ( P < 0 . 001 ) and free IGF 1 ( P < 0 . 001 ) compared to premenopausal women , while plasma IGFBP 2 ( P < 0 . 05 ) and immunoreactive IGFBP 3 ( P < 0 . 001 ) were higher in postmenopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : Direct binding between purified IGFBP 3 and fibronectin and the strong co localization the two proteins in the cartilage matrix support the hypothesis that IGFPB 3 and fibronectin help regulate local IGF 1 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effects of tonsillectomy and adenoidectomy on serum IGF 1 and IGFBP 3 levels in children . ^^^ Most of the circulating IGF 1 is bound to insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ The objective of this study is to determine blood serum levels of IGF 1 and IGFBP 3 in patients with adenoid and tonsillar hypertrophy . ^^^ STUDY DESIGN : The blood serum levels of IGF 1 and its binding protein IGFBP 3 were examined in 41 randomly selected children with a diagnosis of upper airway obstruction resulting from hypertrophic tonsils and adenoids . ^^^ Coated tube immunoradiometric assay ( IRMA ) method was used to analyze IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our aim was to test the sensitivity and specificity of height velocity ( HV ) , IGF 1 , IGFBP 3 and GH stimulation tests alone or in combination in the diagnosis of GHI . ^^^ IGF 1 and IGFBP 3 circulating levels were measured by immunoradiometric assay ( IRMA ) . ^^^ The following cut off values were chosen to discriminate between GHI and nonGHI short children : HV < 25th centile over the 6 12 months prior to the initiation of GH therapy , peak GH responses < 10 or < 7 micro g / l ( < 20 or < 14 mU / l ) and IGF 1 and IGFBP 3 values < 1 . 9 z score . ^^^ Sensitivity was 73 % for IGF 1 and 30 % for IGFBP 3 measurement , whilst specificity was 95 % for IGF 1 and 98 % for IGFBP 3 evaluation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Growth failure in a child showing characteristics of Seckel syndrome : possible effects of IGF 1 and endogenous IGFBP 3 . ^^^ Laboratory investigations at the age of 19 months revealed high levels of IGF 1 , IGF 2 and IGFBP 3 . ^^^ We conclude that in this patient with Seckel syndrome and severe growth impairment IGF 1 resistance is possibly related to altered production of IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting serum androstenedione , testosterone , 17 hydroxyprogesterone , estrone , estradiol , insulin and IGF 1 concentrations were measured by RIA , GH binding protein ( GHBP ) by IFMA and IGF binding protein ( IGFBP ) 1 and IGFBP 3 by IRMA . ^^^ Serum GHBP , IGF 1 and IGFBP 3 concentrations were similar in both groups , whether lean or obese . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , and IGFBP 3 were measured using commercial ELISA kits ( Diagnostic Systems Laboratories , Webster , TX ) . ^^^ Breast cancer patients had higher plasma levels of IGF 1 and IGFBP 3 . ^^^ A dose response relationship was observed between breast cancer risk and the level of IGF 1 or IGFBP 3 . ^^^ The adjusted odds ratios were 2 . 01 ( 95 % confidence interval , 1 . 26 3 . 19 ) or 3 . 01 ( 95 % confidence interval , 1 . 81 4 . 99 ) , respectively , for women with the highest tertile of IGF 1 or IGFBP 3 compared with those with the lowest tertile of these molecules . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured plasma IGF 1 , IGF 2 , and IGF BP 3 in a cross section of 210 men and 171 postmenopausal women enrolled in research as healthy controls . ^^^ Overall , IGF 1 and IGF BP 3 levels did not differ by gender , although men had significantly higher molar ratios of IGF 1 to IGF BP 3 and lower plasma IGF 2 than women without recent HRT use . ^^^ Results for ratios of IGF 1 and IGF 2 to IGF BP 3 generally reflected those for IGF 1 and IGF 2 levels , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Morning serum growth hormone ( GH ) , IGF 1 , IGF binding protein ( BP ) 2 , IGFBP 3 , IGF I / IGFBP 2 , and IGFBP 3 / IGFBP 2 ratios were evaluated before , during ( 8th and 15th days ) , and at the end and after cessation ( +3 , +6 , +9 , +12 , and +15 d ) of a 3 wk treatment with different doses of rhGH ( 0 . 09 IU . kg BW ( 1 ) . d ( 1 ) for 6 or 3 d a week , i . e . , the A and B trials , respectively ) in seven well trained subjects not involved in competitive sports . ^^^ RESULTS : In both trials , significant increases of IGF 1 ( higher in the A trial ) and IGFBP 3 serum concentrations during rhGH administration were observed . ^^^ CONCLUSION : Although IGF 1 and IGFBP 3 seem potentially the most specific markers of rhGH assumption , our data suggest that for antidoping purposes a single evaluation of their absolute serum concentration is not a sufficiently secure method to detect rhGH abuse in all subjects , especially in the case of low rhGH doses . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High levels of plasma insulin like growth factor 1 ( IGF 1 ) and low levels of insulin like growth factor binding protein 3 ( IGFBP 3 ) have been related to increased risk of several cancers . ^^^ The authors examined the relation of anthropometric and behavioral factors to plasma concentrations of IGF 1 and IGFBP 3 in a cross sectional study of 616 Japanese men aged 45 55 years in 1995 1996 . ^^^ In univariate analyses , body mass index was strongly , positively associated with both IGF 1 and IGFBP 3 . ^^^ The waist / hip ratio was also linearly related to IGF 1 and IGFBP 3 up to the third quartile level . ^^^ Height was weakly , positively associated with IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured the serum concentrations of DHEA sulfate ( DHEA S ) , ACTH , cortisol , human GH ( hGH ) , IGF 1 , and IGF 1 binding protein 3 ( IGFBP 3 ) preoperatively and then 2 and 7 days after surgery ; we also compared the preoperative findings with those of a healthy control group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurements of plasma IGF 1 , IGFBP 3 , insulin , and leptin were available for 445 men and 391 women . ^^^ The molar ratio of IGF I / IGFBP 3 showed a similar profile to that of IGF 1 , although much less pronounced . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) potentiates IGF 1 action in the non transformed mammary epithelial cell line , MAC T , via a mechanism that is independent of its ability to bind IGF 1 . ^^^ IGF binding protein 3 ( IGFBP 3 ) potentiates IGF 1 action in the non transformed mammary epithelial cell line , MAC T , via a mechanism that is independent of its ability to bind IGF 1 . ^^^ In MAC T cells constitutively expressing IGFBP 3 ( +BP3 ) , phosphorylation of Akt following stimulation with IGF 1 was enhanced relative to mock transfected cells ( Mock ) . ^^^ The enhanced Akt response was elicited by factors that activate the IGF receptor but exhibit reduced affinity for IGFBP 3 , such as Long R ( 3 ) IGF 1 , B chain IGF 1 and insulin . ^^^ In contrast , [ Leu ( 60 ) ] IGF 1 , which binds IGFBP 3 but has reduced affinity for the IGF receptor , failed to induce comparable activation , suggesting that an association between IGF 1 and IGFBP 3 is not required for the effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Like IGF 1 and IGFBP 3 , serum levels of ALS depend on growth hormone ( GH ) . ^^^ We also measured the levels of serum IGF 1 and IGFBP 3 in these patients to compare the utility of ALS with IGF 1 and IGFBP 3 as a marker of GH secretion . ^^^ Serum ALS was measured by radioimmunoassay ( RIA ) kit , and serum IGF 1 and IGFBP 3 were measured by immunoradiometric assay ( IRMA ) kits in 56 patients with adult GHD ( adult onset ( AO ) / child onset ( CO ) , 13 / 43 ) and 43 patients with acromegaly . ^^^ These data suggested that measurement of ALS offers no advantage over measurements of serum IGF 1 and IGFBP 3 . ^^^ Furthermore , our results indicate that serum IGFBP 3 is the most suitable marker of GH secretion for adult GHD , especially CO , while IGF 1 may be the most useful in acromegaly . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma glucose , insulin , cholesterol , IGFBP 3 , and IGF 1 concentrations were not affected by feeding Propionibacteria , but those variables increased with week postpartum . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND MEASUREMENTS : Serum levels of IGF 1 , IGF 2 and IGFBP 3 , and peripheral markers leptin , C terminal propeptide of type 1 collagen ( PICP ) and N terminal propeptide of type 3 collagen ( PIIINP ) , alkaline phosphatase ( AP ) and osteocalcin ( OC ) were measured at the start and after 2 and 12 weeks of periods of no treatment , GH 1 . 5 IU / m2 and GH 3 . 0 IU / m2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : All four GH doses increased IGF 1 , IGFBP 3 and IGF 1 / IGFBP 3 ratio , and decreased IGFBP 1 from day 3 onwards ( P < 0 . 05 ) . ^^^ The overall increase in the GH dose increased IGF 1 , IGFBP 3 , fasting glucose and insulin ( P < 0 . 001 ) , demonstrated a positive correlation with the final change in fasting glucose ( r = 0 . 5 , P < 0 . 05 ) and insulin ( r = 0 . 8 , P < 0 . 001 ) and a negative correlation with final SI ( r = 0 . 5 , P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood collection on days 1 , 15 and 30 for IGF 1 , IGFBP 3 , IGFBP 2 , bone alkaline phosphatase ( BAP , osteoblast marker ) , procollagen type 1 C terminal propeptide ( PICP , marker of type 1 collagen synthesis ) , procollagen type 3 N terminal propeptide ( P3NP , marker of soft tissue type 3 collagen synthesis ) and type 1 collagen telopeptide ( ICTP , marker of type 1 collagen breakdown ) . ^^^ IGF 1 , IGFBP 3 , BAP , PICP and P3NP were low or very low on day 1 compared to well nourished age matched European children , and all increased within 15 days ( P < 0 . 001 ) , with PICP and P3NP reaching levels higher than European norms . ^^^ Changes in IGF 1 , IGFBP 3 , BAP , PICP and P3NP over 30 days correlated with ponderal growth ( whz change ) over the same period ( all P < 0 . 01 ) . ^^^ At most time points , there were strong positive correlations ( 1 ) among IGF 1 , IGFBP 3 , BAP , PICP and P3NP ( P < 0 . 01 ) and ( 2 ) between IGFBP 2 and ICTP ( P < 0 . 01 ) . ^^^ Conversely , IGFBP 2 was negatively correlated with IGF 1 , IGFBP 3 , BAP , PICP and P3NP at most time points ( P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Since insulin like growth factor ( IGF ) 1 is produced by rat granulosa cells and exerts a synergistic role with FSH on granulosa cell steroidogenesis , we hypothesised that CRH may suppress oestrogen release from granulosa cells by inhibiting IGF 1 release and / or stimulating the release of its binding protein ( IGFBP 3 ) . ^^^ In conclusion , CRH suppressed the release of oestrogen and IGF 1 , but not of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These studies indicate that IGFBP 4 may regulate local metabolism during preadipocyte differentiation , whereas IGFBP 3 may antagonize preadipocyte differentiation by targeting IGF 1 away from differentiating cells and towards growing cells . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 and IGF binding protein 3 were higher in patients with PCOS than in obese controls ( P = 0 . 03 and P = 0 . 02 , respectively ) , but the IGF 1 / IGF binding protein 3 ratio was equivalent in all three study groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 , IGFBP 3 , specifically binds to IGFs with high affinity , but it is also capable of modulating the IGF 1 signalling pathway or inducing apoptosis independently of its binding to IGFs . ^^^ The effect of IGFBP 3 on calcium concentrations was dose dependent and also occurred when IGFBP 3 was complexed with either IGF 1 or heparin , suggesting that the receptor binding site is probably located in the least conserved central domain of IGFBP 3 . ^^^ Insulin like growth factor binding protein 3 , IGFBP 3 , specifically binds to IGFs with high affinity , but it is also capable of modulating the IGF 1 signalling pathway or inducing apoptosis independently of its binding to IGFs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the cross sectional association of plasma IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels with intakes of alcohol , energy , macronutrients , micronutrients , and specific foods in 1037 healthy women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the cross sectional association of plasma IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels with age , smoking , physical activity , and reproductive and menopausal factors in 1037 healthy women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Adjustment for IGF 1 , IGF binding protein ( BP ) 1 , IGFBP 3 , smoking , or body mass index did not substantially alter the association , whereas adjustment for IGFBP 2 moderately attenuated the relationship . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , the double gene disruption LID+ALSKO mouse model demonstrates that a threshold concentration of circulating IGF 1 is necessary for normal bone growth and suggests that IGF 1 , IGFBP 3 , and ALS play a prominent role in the pathophysiology of osteoporosis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal serum GH , GH binding protein ( GHBP ) , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) levels were determined as well as GH levels during GHRH stimulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , cholesterol , triglycerides , HDL cholesterol , LDL cholesterol , apolipoprotein B ( apoB ) , lipoprotein ( a ) ( Lp ( a ) ) , glucose , HbA ( 1c ) , and C peptide were measured in fasting samples . ^^^ RESULTS : Partial correlations adjusted for age , sex , BMI , and diabetes duration showed an inverse association of IGFBP 1 with C peptide ( r = 0 . 24 , P = 0 . 018 ) and with maximal IMT ( r = 0 . 42 , P < 0 . 001 ) , whereas IGF 1 and IGFBP 3 correlated positively with several risk promoting lipid parameters . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have investigated the mitogenic properties of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and epidermal growth factor ( EGF ) on human breast epithelial cells ( HBEC ) in primary culture . ^^^ IGFBP 3 inhibits the stimulation by IGF 1 , IGF 2 and IGF 1 plus EGE In addition , it appears that IGFBP 3 has an inhibitory effect on HBEC growth that is IGF independent . ^^^ This study is the first to address the effects of IGF 1 , IGF 2 and IGFBP 3 alone and in combination with EGF on HBEC growth in primary culture . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although IGF 1 was correlated at baseline to peak oxygen consumption in all subjects , there was a significant decrease in IGF 1 and IGF binding protein 3 in the training subjects ( 12 . 8 + / 7 . 3 % and 17 . 5 + / 7 % , respectively , p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Evidence for compensatory mechanisms to counter the antianabolic inflammatory response to acute exercise were also noted : IL 1ra increased ( 80 + / 20 % ) and IGF binding protein 3 proteolysis ( which can maintain unbound , biologically active IGF 1 despite losses in total IGF 1 ) increased significantly ( 101 + / 39 % ) as well . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , insulin like growth factor 1 ( IGF 1 ) in combination with its principle binding protein ( IGFBP 3 ) equilibrates pro to anti inflammatory cytokine ratios and improves homeostasis of severely burned pediatric patients . ^^^ Five days after excision and grafting , cardiac function was determined and blood samples were taken for serum levels of IGF 1 , IGFBP 3 , creatinine , pre albumin , cholinesterase , pro inflammatory cytokines ( IL 1beta , IL 6 , and TNF ) , and anti inflammatory cytokines ( IL 2 , IL 4 , IL 10 and IFN gamma ) . ^^^ Serum IGF 1 in burned children given the IGF I / BP 3 complex increased from 102 + 15 to 433 + 33 microg / ml and IGFBP 3 increased from 1 . 5 + 0 . 2 to 3 . 0 + 0 . 2 microg / ml ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to investigate the action of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) on steroidogenesis and apoptosis in human corpus luteum during the midluteal phase . ^^^ Slices from corpora lutea were incubated for 4 h with IGF 1 or IGFBP 3 . ^^^ Neither IGF 1 nor IGFBP 3 had an effect on P ( 450arom ) expression or on the concentrations of its substrates . ^^^ In conclusion , these results indicate that IGF 1 and IGFBP 3 act as regulatory peptides of the function of the human corpus luteum during the midluteal phase . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied serum ALS , free IGF 1 , total IGF 1 , IGFBP 3 levels and IGFBP 3 protease activity in 13 girls , aged 1 . 6 7 . 8 yr ( mean , 5 . 9 + / 2 . 2 ) , diagnosed as having CPP before and after 6 and 12 months of GnRH analog ( GnRHa ) therapy . ^^^ Serum IGF 1 and IGFBP 3 were also increased before treatment , but both of these factors remained elevated after 6 and 12 months of GnRH A therapy [ IGF 1 SD score , 3 . 20 + / 1 . 64 , 2 . 92 + / 1 . 82 , and 3 . 68 + / 1 . 94 ( P = NS ) , respectively ; IGFBP 3 SD score , 1 . 02 + / 0 . 53 , 0 . 94 + / 0 . 68 , and 1 . 22 + / 0 . 87 ( P = NS ) , respectively ] . ^^^ Serum free IGF 1 levels and IGFBP 3 proteolytic activity did not vary significantly from their pretreatment values during GnRHa therapy . ^^^ Serum IGF 1 and IGFBP 3 levels were also increased before therapy , but their levels were not influenced by treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Children with idiopathic short stature showed a better response in IGFBP 3 generation compared with that previously observed with IGF 1 , reaching concentrations in normal range with either dose of GH , suggesting that any GHI in this group is relatively limited to IGF 1 production . ^^^ Failure to raise both IGF 1 and IGFBP 3 lowered sensitivity to 82 86 % with low dose GH , and 86 91 % with high dose GH . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that the relative insulin resistance of puberty is associated with changes in IGF 1 levels , we compared IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and IGFBP 1 levels to insulin resistance [ M ( lbm ) , milligrams glucose used per kilogram of lean body mass ( LBM ) per minute ] measured during euglycemic , hyperinsulinemic clamp studies in 342 children and adolescents . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The aim of the study was to evaluate whether the circulating levels of insulin like growth factor 1 ( IGF 1 ) and its major circulating binding protein , IGFBP 3 , are affected in premature rupture of membranes ( PROM ) and preterm delivery . ^^^ METHODS : The levels of IGF 1 and IGFBP 3 were measured in 32 pregnant women with PROM and in 27 healthy gestational age matched pregnant women . ^^^ In the PROM patients , the serum IGF 1 and IGFBP 3 levels ( 289 + / 21 ng / ml and 8248 + / 407 ng / ml , respectively ) were not statistically different from those in the control subjects ( 275 + / 22 ng / ml and 7579 + / 488 ng / ml ) . ^^^ Also in this subgroup of patients the levels of serum IGF 1 ( 281 + / 27 ng / ml ) and IGFBP 3 ( 9010 + / 633 ng / ml ) were not different from those in the control subjects . ^^^ No consistent changes in IGF 1 or IGFBP 3 concentrations were seen during the mean follow up period of 9 days . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal plasma concentrations of GH , IGF 1 , IGFBP 3 , and IGFBP 1 did not significantly differ between CHI patients and controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Intact IGFBP 1 , IGFBP 2 and IGFBP 6 inhibited in equimolar concentration the IGF 1 and IGF 2 stimulated DNA synthesis and cell proliferation , whereas the biological activity of IGFBP 3 was complex . ^^^ It had an IGF independent antiproliferative effect and also inhibited IGF dependent chondrocyte proliferation under coincubation conditions , whereas under preincubation conditions IGFBP 3 enhanced IGF 1 responsiveness . ^^^ Studies on the mechanism by which IGFBP 3 potentiated IGF activity demonstrated that under preincubation conditions IGFBP 3 is capable to associate with the cell membrane and to facilitate IGF 1 cell surface binding . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to compare circulating levels of growth hormone ( GH ) , IGF 1 , and IGF binding protein ( IGFBP ) 1 and IGFBP 3 in response to a long duration endurance exercise in trained vs . sedentary middle aged males and to determine whether a relationship with glucose homeostasis exists . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The Tg mice of both lineages demonstrated increased serum levels of human ( h ) IGFBP 3 and total IGF 1 compared with wild type ( Wt ) mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Protocols 3 and 4 were the most effective in increasing serum IGF 1 and IGFBP 3 , whereas protocols administering pulsatile GH had the greatest effects on markers of bone formation and resorption . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
His evaluation showed growth hormone ( GH ) deficiency with low insulin like growth factor 1 ( IGF 1 ) , low IGF binding protein 3 , and low GH response to stimulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , we measured the concentration of IGF 1 and its major binding protein , IGF binding protein 3 ( IGFBP 3 ) , in serum from 127 patients with multiple myeloma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Because one of the functions of IGFBPs is to modulate IGF 1 action on muscle size and phenotype , it is hypothesized that IGFBP 3 and 5 proteins may have potential modulatory roles in type 1 fiber dominated muscles , aging , and regrowth from atrophy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In both mid and late pregnancy , maternal food restriction reduced maternal plasma IGF 1 by 56 per cent ( P < 0 . 0005 ) and 50 per cent ( P < 0 . 0005 ) respectively , and plasma IGFBP 3 by 47 per cent ( P=0 . 03 ) and 55 per cent ( P=0 . 002 ) , respectively . ^^^ Late in pregnancy , food restriction reduced the ratio of maternal circulating IGF 1 to IGFBP 1 by 52 per cent ( P=0 . 011 ) and increased the ratio of IGF 1 to IGFBP 3 in maternal plasma by 10 per cent ( P=0 . 011 ) . ^^^ In mid pregnancy , the volume density of the maternal blood space in the placental labyrinth correlated positively with both maternal plasma IGF 1 and IGFBP 3 , while maternal blood space volume correlated negatively with maternal plasma IGFBP 1 . ^^^ In late pregnancy therefore , increased trophoblast abundance and placental vascularity , and a reduced barrier to diffusion between maternal and foetal blood , occurs in association with increased abundance of IGF 1 and its major carrier , IGFBP 3 , and a reduction in that of IGFBP 1 in maternal blood in the guinea pig . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin resistance ( HOMA ) in relation to plasma cortisol , IGF 1 and IGFBP 3 . ^^^ OBJECTIVE : To investigate the possible contribution of plasma cortisol and growth hormone ( GH ) as reflected by insulin like growth factor 1 ( IGF 1 ) / insulin like growth factor binding protein 3 ( IGFBP 3 ) on insulin action in short statured children . ^^^ HOMA was examined in relation to fasting plasma cortisol , IGF 1 , IGFBP 3 and in addition to birthweight and body mass index ( BMI ) . ^^^ Only in normal short statured children IGF 1 ( r = 0 . 338 , p < 0 . 03 ) and IGFBP 3 ( r = 0 . 493 , p < 0 . 002 ) were associated with insulin resistance . ^^^ In normal short statured children HOMA was associated with IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like Growth Factor 1 ( IGF 1 ) and Insulin like Growth Factor Binding Protein 3 ( IGFBP 3 ) , were measured in 7 patients in the study group and 5 in the control group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 levels in severe iodine deficiency . ^^^ Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , thyroxine ( T 4 ) , and thyroid stimulating hormone ( TSH ) were also analyzed to investigate the mechanisms by which iodine depletion leads to growth failure . ^^^ Pubertal children in a severe iodine deficient SID area had lower height standard deviation scores ( HSDS ) , IGF 1 and IGFBP 3 levels than those living in mild iodine deficient MID area . ^^^ The major determinants of HSDS were age , IGF 1 , IGFBP 3 and TSH . ^^^ IGF 1 and IGFBP 3 were negatively correlated with T 4 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels were measured before , after 6 and 12 months of GH , and correlated with the GH dose used . ^^^ IGF 1 SDS increased significantly during the first 6 months of therapy ( P < . 0003 ) , but increased no further at 12 months despite the use of a higher GH dose ( 0 . 1 vs 0 . 14 IU / kg / day ) , whereas IGFBP 3 SDS increased at both 6 and 12 months . ^^^ There was no correlation between the GH dose used and IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was also a correlation between serum levels of IGF 1 and IGFBP 3 and an inverse correlation between the serum level of IGF 1 and serum levels of IGFBP 1 and IGFBP 2 in the ANS group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To clarify the impairment of the GH / IGF 1 axis in obstructive sleep apnea syndrome ( OSAS ) , in 13 adult male patients with OSAS ( OSA ) as well as 15 weight matched patients with simple obesity ( OB ) and 10 normal lean male subjects ( NS ) , we studied : 1 ) the GH response to GHRH ( 1 micro g / kg 4 ) plus arginine ( 30 g 4 ) ; and 2 ) the IGF 1 and IGF binding protein 3 responses to a very low dose recombinant human ( rh ) GH treatment ( 5 . 0 microg / kg sc per day for 4 d ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Differences in sexual development , BMI , FM , insulin sensitivity , and IGFBP 3 could explain the difference in IGF 1 between TS and control subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In agreement with the Northern data , IGF 1 mRNA levels in Dex treated cultures were lower than in control cultures at all three time points , and IGFBP 3 levels were lower in Dex treated cultures at day 20 of culture . ^^^ Some of these changes are dependent on the stages of development ( e . g . , regulation of IGFBP 3 and 4 ) and some remain similar trends at all stages ( e . g . , regulation of IGF 1 and the three receptors ) . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the usefulness of serum IGF 1 or IGF binding protein 3 for predicting pathology results in men undergoing prostate biopsy is unclear . ^^^ We examined the relationships of serum IGF 1 , IGF binding protein 3 and the results of prostate biopsy . ^^^ PSA , free PSA , IGF 1 and IGF binding protein 3 were measured . ^^^ Adjustment for age and PSA showed significantly lower IGF 1 in cancer cases , while IGF binding protein 3 was not significant . ^^^ ROC values were significantly higher for free to total PSA and PSA than for crude and age adjusted IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , des ( 1 3 ) IGF 1 , which weakly binds IGF binding protein 3 ( IGFBP 3 ) , induced IGF IR phosphorylation . ^^^ The findings also show a role for IGFBP 3 in the inhibition of IGF 1 signaling in hepatocytes . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this prospective , observational study IGF 1 , IGF 2 , IGF binding protein 2 , IGF binding protein 3 , and acid labile subunit were measured by immunoassays in the wound fluid and plasma of young ( 23 . 5 + / 3 . 3 years ) and elderly ( 78 . 9 + / 6 . 2 years ) patients before and daily for 4 days after elective surgery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Pituitary height ( PH ) and thickness of the pituitary stalk ( PS ) were measured and their relationship with the maximum GH peak during a GH stimulation test , serum IGF 1 and IGFBP 3 levels was evaluated . ^^^ A moderate decrease in PH was associated with significantly lower maximum serum GH peaks and lower serum IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the relation of body mass index , fasting and 2 h postload plasma glucose levels and plasma concentrations of IGF 1 and IGFBP 3 to colorectal adenomas in middle aged Japanese men . ^^^ After adjustment for rank in the SDF , hospital , smoking and IGFBP 3 , a statistically nonsignificant modest increase in the prevalence odds of colorectal adenomas was observed for the highest versus the lowest quartile level of IGF 1 . ^^^ Men with high levels of IGFBP 3 showed only a minimal decrease in risk after adjustment for IGF 1 . ^^^ Fasting and 2 h glucose and body mass index were more strongly positively associated with colorectal adenomas than IGF 1 , especially with advanced adenomas , independently of IGF 1 and IGFBP 3 . ^^^ The findings suggest that plasma IGF 1 and IGFBP 3 may be involved in colorectal tumorigenesis regardless of the stage in growth of adenoma , but not as a mediator for the effects of being overweight or of hyperglycemia . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were harvested at 3 , 10 and 17 PBDs for the determination of serum growth hormone , insulin like growth factor ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , serum proteins , plasma insulin , plasma glucagons and blood glucose , which were then compared and analyzed between two the groups . ^^^ RESULTS : The serum levels of GH , IGF 1 , IGFBP 3 , serum prealbumin and transferrin in rhGH treatment group were evidently higher than those in control groups at 10 and 17 PBDs ( P < 0 . 05 approximately 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting levels of glucose , insulin , proinsulin ( P ( 0 ) ) , hemoglobin A1c , testosterone , SHBG , delta 4 androstenedione , dehydroepiandrosterone sulfate , LH , FSH , IGF 1 , IGF binding protein 1 , IGF binding protein 3 , free IGF 1 , and lipids were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurement of the ratio of IGF 1 to IGF binding protein 3 in follicular fluids indicated that there was not a disproportionate increase in 1 binding protein 3 in animals infused with either IGF 1 alone or IGF 1 + hGH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 transport in the rat heart . ^^^ IGFBP 3 , uptake of ( 125 ) 1 IGF 1 was decreased to 41 % , which was significantly greater than LR ( 3 ) and ( 125 ) 1 IGF 1 ( 41 vs . 9 % ) . ^^^ These data suggest that both microvessel IGF 1 and IGFBP 3 binding sites contribute to the transport of IGF 1 in the perfused rat heart . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 as predictors of advanced stage prostate cancer . ^^^ To determine whether plasma levels of IGF 1 and its main circulating binding protein , IGF binding protein 3 ( IGFBP 3 ) , predict more aggressive forms of prostate cancer , we investigated the association between plasma levels of each and specific stages and grades of prostate cancer . ^^^ IGF 1 and IGFBP 3 were measured in prospectively collected plasma samples . ^^^ Conditional logistic regression models were used to estimate the relative risks ( RRs ) for prostate cancer associated with IGF 1 and IGFBP 3 , stratified on grade ( Gleason score > or = 7 versus < 7 ) and stage ( early = stage A or B prostate cancer versus advanced = stage C or D prostate cancer ) . ^^^ RESULTS : Plasma levels of IGF 1 and IGFBP 3 were predictors of advanced stage prostate cancer ( RR = 5 . 1 , 95 % confidence interval [ CI ] = 2 . 0 to 13 . 2 for highest versus lowest quartiles of IGF 1 ; RR = 0 . 2 , 95 % CI = 0 . 1 to 0 . 6 for highest versus lowest quartiles of IGFBP 3 ) but not of early stage prostate cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in 194 case subjects who subsequently died from lung cancer during an 8 year follow up and in 9351 controls . ^^^ The risk was increased in the highest vs . the lowest quartile of IGF 1 only after controlling for IGFBP 3 ( OR , 1 . 74 ; 95 % CI , 1 . 08 2 . 81 ) . ^^^ Limiting subjects to those followed for 3 years strengthened the negative associations of IGF 2 and IGFBP 3 , whereas the ORs for IGF 1 generally decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 , insulin , glucose , and IGFBP 1 , IGFBP 2 , and IGFBP 3 in serum drawn at least 2 years before diagnosis ( mean times of 10 . 5 and 15 . 8 years for pre and postmenopausal cases , respectively ) were compared using conditional logistic regression analysis . ^^^ After adjusting for insulin , glucose , body mass index , and IGF 1 , premenopausal women in the highest quartile of IGFBP 3 had an elevated risk of breast cancer [ odds ratio ( OR ) = 5 . 28 , 95 % confidence interval ( CI ) = 1 . 13 24 . 7 ] . ^^^ In premenopausal women , elevated serum IGF 1 and IGFBP 3 are associated with increased breast cancer risk , whereas elevated serum IGFBP 2 is inversely associated with risk of postmenopausal breast cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) play an important role in regulation of prostate cancer cell growth . ^^^ We studied the prognostic significance of serum IGF 1 and IGFBP 3 levels , and IGF 1 / prostate specific antigen ( PSA ) and IGFBP 3 / PSA ratios in patients with prostate cancer . ^^^ METHODS : Serum levels of IGF 1 , IGFBP 3 , and PSA were determined in 112 patients diagnosed with prostate cancer . ^^^ Univariate analysis showed that serum PSA , IGF 1 / PSA ratio , IGFBP 3 / PSA ratio , T , N and M classifications correlated significantly with relapse free survival of advanced prostate cancer patients treated with hormonal therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
When IGF binding protein 3 ( an inhibitor of IGF 1 bioactivity ) was added in combination with 25 micro M celecoxib , enhanced growth inhibition was observed ( approximately 60 % decrease in cell viability ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of testosterone ; estrone ; estradiol ; dehydroepiandrosterone and dehydroepiandrosterone sulfate ; insulin like growth factor 1 ( IGF 1 ) ( total and free ) and its binding proteins IGFBP 1 , IGFBP 2 , and IGFBP 3 ; and leptin were measured . ^^^ Serum estradiol ; dehydroepiandrosterone sulfate ; total IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 ; and leptin showed no association . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 bound with high affinity to recombinant IGFBP 3 and IGFBP 4 with K ( D ) s of < 0 . 25 nmol . ^^^ As reported for native IGFBPs , IGF 2 bound with affinity higher than IGF 1 to recombinant IGFBP 3 and IGFBP 4 ( K ( D ) of < 0 . 05 nmol ) . ^^^ Recombinant IGFBP 3 and IGFBP 4 were found to inhibit the IGF induced proliferation of an NIH3T3 cell line engineered to overexpress the IGF 1 receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ' replacement ' in diabetes is under investigation and new methods of delivering IGF 1 as a complex with IGFBP 3 offer exciting new prospects . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Cross sectional study : urinary leptin , measured in the first morning urine voided and expressed as ng excreted overnight , and serum concentrations of leptin , IGF 1 , IGF 2 , IGFBP 3 and IGFBP 1 were determined in a cohort of 188 healthy schoolchildren aged 5 19 years ( 88 males , 100 females ) . ^^^ In the cross sectional study urinary leptin correlated significantly with serum IGF 1 ( r = +0 . 41 , P = 0 . 001 ) , IGF 2 ( r = +0 . 19 , P = 0 . 05 ) , IGFBP 3 ( r = +0 . 29 , P = 0 . 001 ) and IGFBP 1 ( r = 0 . 25 , P = 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Pretreatment with insulin like growth factor 1 ( IGF 1 ) was performed to determine whether the effects of insulin like growth factor binding protein 3 ( IGFBP 3 ) on apoptosis were IGF dependent . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum T and F IGF 1 and IGF 2 were determined using noncompetitive time resolved monoclonal immunofluorometric assays , IGFBP 1 was determined by an in house RIA , IGFBP 2 by a novel in house time resolved immunofluorometric assay , whereas IGFBP 3 by an immunoradiometric assay . ^^^ Specifically , mean T IGF 1 for patients group was 131 ( 61 ) , F IGF 1 was 0 . 47 ( 0 . 16 ) , T IGF 2 was 1056 ( 300 ) , F IGF 2 was 1 . 45 ( 0 . 54 ) , IGFBP 1 was 33 ( 14 ) , IGFBP 2 was 848 ( 377 ) , and finally IGFBP 3 was 3953 ( 1422 ) . ^^^ For controls , mean T IGF 1 was 146 ( 51 ) , F IGF 1 was 0 . 85 ( 0 . 43 ) , T IGF 2 was 939 ( 197 ) , F IGF 2 was 1 . 53 ( 0 . 53 ) , IGFBP 1 was 44 ( 24 ) , IGFBP 2 was 764 ( 316 ) and finally IGFBP 3 was 3721 ( 1017 ) . ^^^ Furthermore , no statistically differences emerged in the ratio between molar weights of T IGF I / IGFBP 3 and T IGF II / IGFBP 3 , as well as to the F / T IGF 1 and F / T IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Samples were assayed for LH , FSH , estradiol ( E 2 ) , glucose , beta hydroxybutyrate , insulin , cortisol , GH , IGF 1 , IGF 1 binding protein ( IGFBP ) 1 , IGFBP 3 , leptin , and T 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood was collected on a single day during the follicular phase ( d 2 6 ) of each menstrual cycle for analysis of TSH , insulin , total T 3 , total T 4 , free T 4 , leptin , hGH , IGF 1 , and IGF binding protein ( IGFBP ) 1 and IGFBP 3 . ^^^ IGF 1 , IGFBP 1 , IGFBP 3 , IGF I / IGFBP 1 , IGF I / IGFBP 3 , and hGH were comparable among the different categories of cycles . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The serum concentrations of total GH , GH 22 kDa , IGF 1 , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , acid labile subunit , procollagen type 3 ( P 3 P ) , and the bone markers osteocalcin , carboxy terminal cross linked telopeptide of type 1 collagen ( ICTP ) , and carboxy terminal propeptide of type 1 procollagen were measured . ^^^ The components of the IGF 1 axis showed a similar pattern , with a peak value after exercise compared with baseline for IGF 1 ( P < 0 . 001 , males and females ) ; IGFBP 3 ( P < 0 . 001 , males and females ) ; acid labile subunit [ P < 0 . 001 , males ; not significant ( NS ) , females ] , and IGFBP 2 ( P < 0 . 05 , females ; NS , males ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum calcium , phosphate , alkaline phosphatase , aspartate aminotransferase , alanine aminotransferase , gamma glutamyltransferase , bilirubin , urea , creatinine , uric acid , proteins , parathormone , vitamin D ( 3 ) , IGF 1 , IGF BP 3 and telopeptide , as well as urinary telopeptide , were measured at baseline and 1 , 3 , 6 , 9 and 12 months after surgery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition of exogenous IGFBP 5 and IGFBP 3 to MECs suppressed IGF 1 mediated survival , resulting in threefold greater apoptosis in cells incubated with IGF 1 and IGFBP 5 compared with IGF 1 alone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PURPOSE : Because insulin like growth factors ( IGFs ) and their binding proteins have been implicated in the development of prostate , breast , colon and lung cancer , we examined the role of IGF 1 and IGF binding protein 3 levels in bladder cancer risk . ^^^ MATERIALS AND METHODS : We used an enzyme linked immunosorbent assay to compare plasma levels of IGF 1 and IGF binding protein 3 in 154 patients with bladder cancer and 154 controls from an ongoing case control study . ^^^ The highest quartile plasma levels of IGF 1 were associated with an increased risk of bladder cancer ( OR 3 . 10 , 95 % CI 1 . 43 to 6 . 70 ) and the highest quartile plasma levels of IGF binding protein 3 were associated with a reduced risk of bladder cancer ( OR 0 . 38 , 95 % CI 0 . 19 to 0 . 78 ) . ^^^ The effects were more striking when IGF 1 and IGF binding protein 3 levels were analyzed together . ^^^ In addition , a higher molar ratio of IGF 1 to IGF binding protein 3 was associated with an increased risk of bladder cancer ( OR 4 . 30 , 95 % CI 1 . 99 to 9 . 28 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of insulin like growth factor 1 ( IGF 1 ) , IGFs complexed to specific , structurally homologous binding proteins ( IGFBP 3 ) , cortisol , free thyroxine and tumor necrosis factor alpha ( TNF alpha ) were measured before the start of therapy and after 24 weeks . ^^^ In addition serum IGF 1 and IGFBP 3 values were determined after 48 weeks . ^^^ Baseline serum of IGF 1 and IGFBP 3 concentrations were normal , but IGF 1 tended to be lower than IGFBP 3 . ^^^ IGFBP 3 decreased after 48 weeks whereas IGF 1 stabilized . ^^^ The combination of relatively high serum IGFBP 3 concentration and relatively lower serum IGF 1 suggests the presence of a growth hormone resistant state . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma GH levels were unchanged during the entire course of the study , but a decrease in serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and IGF binding protein 4 ( IGFBP 4 ) occurred after 10 , 30 , and 48 days . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 levels were measured by radioimmunoassay , and IGFBP 3 levels were measured by immunoradiometric assay . ^^^ CONCLUSIONS : Circulating IGFBP 3 levels were low during active IBD and increased at remission in parallel with the IGF 1 levels . ^^^ The IGFBP 3 to IGFBP 2 ratio was altered in the presence of active disease in a manner that would reduce IGF 1 action . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma and pituitary GH concentrations and liver GH receptor ( GHR ) , IGF 1 and IGF binding protein 3 ( IGFBP 3 ) mRNA expression were determined in brushtail possum ( Trichosurus vulpecula ) pouch young aged 12 150 days post partum and in adults . ^^^ Expression of GHR , IGF 1 and IGFBP 3 mRNAs was determined by semi quantitative RT PCR . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of recombinant porcine IGF binding protein 3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF 1 . ^^^ Consequently , the goal of this study was to assess the IGF 1 dependent and IGF 1 independent actions of recombinant porcine IGFBP 3 on proliferation of cultured porcine myogenic cells . ^^^ To facilitate these investigations , we have expressed porcine IGFBP 3 in the baculovirus system , purified and characterized the expressed recombinant porcine IGFBP 3 ( rpIGFBP 3 ) , and produced and characterized an anti porcine IGFBP 3 antibody that neutralizes the biological activity of porcine IGFBP 3 . rpIGFBP 3 suppressed IGF 1 stimulated proliferation of PEMCs in a concentration dependent manner with equimolar concentrations of IGF 1 and rpIGFBP 3 , resulting in complete suppression of IGF 1 stimulated proliferation . rpIGFBP 3 also suppressed Long R 3 IGF 1 stimulated proliferation of PEMC , indicating that rpIGFBP 3 possesses IGF independent activity in this cell system . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Length , insulin like growth factor ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP 3 ) , bone metabolic markers , and vertebral bone density by DXA were evaluated at 6 month intervals . ^^^ All participants had positive IGF 1 , IGFBP 3 , osteocalcin , and bone specific alkaline phosphatase responses . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The objective of this present paper was to analyse the dosages of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 1 and 3 ( IGFBP 1 and IGFBP 3 ) in the follicular fluid environment of infertile patients with endometriosis . ^^^ RESULTS : Follicular fluid IGF 1 and IGFBP 3 levels were not significantly different among the groups ; however , follicular fluid IGFBP 1 levels were lower in those patients with moderate / severe endometriosis ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To quantify the rate of synthesis of insulin like growth factor binding protein 3 ( IGFBP 3 ) and insulin like growth factor 1 ( IGF 1 ) by in vitro cultures of normal and osteoarthritic ( OA ) human articular cartilage . ^^^ METHODS : Levels of IGF 1 and IGFBP 3 in media from in vitro cultures of human cartilage were determined by radioimmunoassay ( RIA ) . ^^^ Ultrafiltration and RIA analysis of synovial fluid ( SF ) samples and washings of cartilage samples ex vivo were used to calculate partition coefficients and to estimate the amount of IGF 1 and IGFBP 3 in cartilage in vivo . ^^^ IGFBP 3 synthesis was stimulated by exogenous IGF 1 . ^^^ The quantities of IGF 1 and IGFBP 3 present ex vivo were 11 . 3 and 78 . 7 ng / gm of cartilage in normal cartilage and 21 . 6 and 225 . 4 ng / gm in OA cartilage . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Secondary aims were to characterize the changes in insulin like growth factors 1 and 2 ( IGF 1 and 2 ) and IGF binding protein 3 ( IGFBP 3 ) in response to exercise training . ^^^ No training induced differences in IGF 1 , IGF 2 , IGF l : IGF 2 ( IGF 1 : IGF 2 ) , and IGFBP 3 were detected . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : The expression of IGF 2 mRNA , IGF 1 R mRNA and IGFBP 3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We observed an increase in leptin in the fourth decade with a decrease in IGF 1 , free IGF 1 and IGFBP 3 throughout the decades . ^^^ Leptin concentrations increased in the last decades and total IGF 1 , free IGF 1 and IGFBP 3 decreased by decade without changes in basal TSH concentration . ^^^ In men , there was a positive correlation between leptin and BMI , waist / hip ratio , total body water , fat free mass and fat mass , and these anthropometric and body composition variables showed a negative correlation with free IGF 1 and IGFBP 3 , without any correlation with total IGF 1 . ^^^ In women , there was a positive correlation between leptin and BMI , waist / hip ratio , total body water , fat free mass , and fat mass , which showed a negative correlation with total IGF 1 and IGFBP 3 , without any correlation with free IGF 1 . ^^^ In men , total IGF 1 was negatively correlated with waist / hip ratio without any correlation with the other variables and free IGF 1 was negatively correlated with BMI and waist / hip ratio , and IGFBP 3 did not show any correlation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Basal serum levels of IGF 1 ( P < 0 . 0001 ) and IGFBP 3 ( P < 0 . 01 ) declined with age , but serum acid labile subunit ( ALS ) levels did not ( P = 0 . 2 ) . ^^^ However , the increment in IGF 1 , IGFBP 3 and ALS in response to GH did not decline with age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , we studied dietary influences on plasma IGF 1 and IGF 1 : IGF binding protein 3 ratio in 753 men in the Health Professionals Follow Up Study who completed a food frequency questionnaire . ^^^ In this generally well nourished population of middle aged to elderly men , plasma IGF 1 and IGF 1 : IGF binding protein 3 molar ratio tended to increase with higher intake of protein and minerals , including potassium , zinc , magnesium , calcium , and phosphorus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of immunoreactive IGF 1 , free IGF 1 , IGFBP 2 and IGFBP 3 were measured before and after treatment and compared to controls matched for age , sex and puberty . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurement of IGF 1 , IGFBP 3 and free IGF 1 levels by ELISA in growth hormone ( GH ) deficient children before and after GH replacement . ^^^ Serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels reflect the growth hormone ( GH ) status . ^^^ We retrospectively studied the changes of serum IGF 1 , serum IGFBP 3 , and plasma free IGF 1 levels in growth hormone deficient ( GHD ) children before and after treatment with recombinant human growth hormone ( rhGH ) for a period of 6 months and 1 year . ^^^ Furthermore , their circulating levels would be modified by serum IGF 1 status , and possibly , IGFBP 3 protease activity . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin , IGF 1 , IGFBP 1 , and IGFBP 3 were measured by ELISA , and serum was used to stimulate LNCaP cell growth in vitro . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGFBP 3 and IGFBP 1 concentrations were measured by IRMA . ^^^ The IGF I / IGFBP 3 ratio , an estimate of biologically active IGF 1 , was very significantly higher in patients with type 1 cysts than in both type 2 patients and healthy women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the effects of combined rosiglitazone and clomiphene citrate versus clomiphene citrate monotherapy on serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels in polycystic ovary syndrome ( PCOS ) and to evaluate these therapeutic interventions in the link between hyperinsulinemia and hormonal perturbations in PCOS . ^^^ Both types of treatment induced a significant decrease in LH , LH : FSH ratio , free testosterone , and IGF 1 : IGBP 3 ratio and were associated with a significant increase in IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Correlation of preoperative levels of IGF 1 and IGFBP 3 with pathologic parameters and clinical outcome in patients with bladder cancer . ^^^ OBJECTIVES : To test the hypothesis that plasma insulin growth factor ( IGF ) 1 or IGF binding protein ( BP ) 3 levels would differ between patients with bladder cancer and healthy controls and whether preoperative plasma IGF 1 or IGFBP 3 could predict the pathologic characteristics and clinical outcome of patients with bladder cancer undergoing radical cystectomy . ^^^ The preoperative plasma levels of IGF 1 and IGFBP 3 were measured using DSL enzyme linked immunosorbent assays . ^^^ The IGF 1 and IGFBP 3 levels were also measured in 44 healthy men . ^^^ RESULTS : The plasma IGF 1 and IGFBP 3 levels in patients with bladder cancer were not significantly different from those in healthy subjects ( P > or=0 . 339 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Treatment with 20 ng / ml tumour necrosis factor alpha ( TNFalpha ) , which inhibits myoblast differentiation , blocked IGFBP 3 production and secretion whereas 30 ng / ml IGF 1 , which stimulates myoblast differentiation , increased IGFBP 3 secretion . ^^^ The TNFalpha induced decrease in IGFBP 3 production and inhibition of differentiation could not be rescued by addition of IGF 1 . ^^^ LongR ( 3 ) IGF 1 , which does not bind to the IGFBPs , had a similar effect on differentiation and IGFBP 3 secretion as IGF 1 , both with and without TNFalpha , confirming that increased IGFBP 3 is not purely due to increased stability conferred by binding to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a subgroup of diabetic patients ( n =21 ) and non diabetic patients ( n =13 ) , vitreous and serum total IGF 1 , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 were also determined . ^^^ Serum and vitreous levels of free IGF 1 , total IGF 1 , IGFBP 1 and IGFBP 3 were measured by immunological methods . ^^^ The relatively lower free IGF 1 level observed in group A could not be attributed to differences in the distribution of intravitreous IGFBP 1 and IGFBP 3 in relation to total IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma glucose , glucagon , growth hormone , cortisol , IGF 1 , and IGF binding protein 3 responses were not different between trials within the 2 h time frame . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition of recombinant human IGFBP 3 or antibodies against IGF 1 or IGF IR significantly inhibited proliferation of KF . ^^^ The bioavailability of IGF 1 may be related to the levels of IGFBP 3 produced , which in turn influences KF proliferation , suggesting that modulation of IGF 1 , IGF IR , and IGFBP 3 , individually or in combination , may represent novel approaches to the treatment of keloids . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We found a significant difference in IGFBP distribution between treatments and , given that IGFBPs regulate IGF 1 activity and that IGF 1 independent activities have been suggested for IGFBP 3 , suggest that shear stress may indirectly regulate IGF 1 activity , and , by extension , the effect of IGF 1 on vascular pathologies . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The results were analyzed through multiple logistic regression and controlled for known risk factors for endometrial cancer , leptin , as well as major components of the IGF system ( IGF 1 , IGF 2 , and IGF binding protein 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effects of recombinant human insulin like growth factor ( IGF ) 1 and estrogen administration on IGF 1 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 in anorexia nervosa : a randomized controlled study . ^^^ Administration of recombinant human ( rh ) IGF 1 has been shown to have positive effects on bone density in anorexia nervosa , but the effects of rhIGF 1 and estrogen on IGF binding protein ( IGFBP ) 2 and IGFBP 3 in anorexia nervosa are not known . ^^^ IGFBP 3 correlated with total hip bone density ( r = 0 . 47 , P = 0 . 0002 ) and was a significant predictor of hip bone density ( P = 0 . 010 ) independent of IGF 1 and body mass index in a multivariate regression model . ^^^ In contrast , no significant effect of estrogen was seen on IGF 1 , IGFBP 2 or IGFBP 3 . ^^^ In conclusion , our data suggest that chronic rhIGF 1 administration increases IGF 1 and IGFBP 2 and decreases IGFBP 3 in women with anorexia nervosa . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Authors investigated serum levels of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , sex hormone binding globulin ( SHBG ) and levels of insulin during oral glucose tolerance test in 34 girls with premature adrenarche in 38 age and BMI matched healthy controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of ACTH , aldosterone , gonadotrophin alpha subunits , AVP , C peptide , estradiol , FSH , GH , glucagon , IGF 1 , IGFBP 3 , insulin , leptin , LH , prolactin , PTH and VIP were measured and the results compared to baseline values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The clinical activity and random GH , IGF 1 , IGFBP 3 , PRL , TSH , free T 4 and lanreotide levels serum concentrations were measured under basal conditions and in the 10 days before surgery . ^^^ RESULTS : The presurgical treatment improved the symptoms of acromegaly and induced a statistically significant reduction of GH , IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In pancreatitis tissue , a significant increase in IGF 1 and IGFBP 3 content was accompanied by a distinct increase in IGF 1 receptor expression , compared to control pancreas tissue . ^^^ In contrast , serum from patients with pancreatitis did not show significant increases in IGF 1 and IGFBP 3 levels , however , significant increases in IGFBP 1 level ( 2 . 5 fold ) . ^^^ Pancreatic cancer tissue and serum of patients with pancreatic cancer showed significant increases in IGF 1 , IGFBP 3 and IGFBP 1 content , accompanied by dramatic increases in IGF 1 tissue receptor expression , compared to controls . ^^^ The data suggest that disturbances in tissue collagen metabolism during pancreatic diseases may result from deregulation of IGF 1 homeostasis and that elevated serum levels of IGF 1 , IGFBP 3 and IGFBP 1 may serve as markers of pancreatic cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Viral load , CD 4 count , interleukin 6 ( IL 6 ) , growth hormone , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) , IGFBP 3 proteolysis , osteocalcin in blood and N terminal telopeptide of type 1 collagen in urine were determined . ^^^ Low IGF 1 was found in only six children , who had low CD 4 count and high IL 6 levels , with normal levels of IGFBP 3 and ALS , absent IGFBP 3 proteolysis and decreased bone mineral density , irrespective of viral load or growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After a single hydrodynamic administration of naked plasmid DNA containing the human growth hormone ( hGH ) gene controlled by an ubiquitin promoter , sustained elevation of circulating hGH was observed the entire observation period ( 68 days ) , with a concomitant normalization of circulating insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels were investigated in 31 children living in an endemic goiter area and 33 healthy subjects living in an nonendemic area . ^^^ Serum IGF 1 and IGFBP 3 levels of iodine and selenium deficient children were found to be lower than those of control subjects ( p < 0 . 001 ) . ^^^ There was also positive correlation between the IGF 1 and IGFBP 3 . ^^^ These results suggest that IGF 1 and IGFBP 3 levels are affected by thyroid dysfunction as a result of iodine and selenium deficiency . ^^^ However , IGF 1 and IGFBP 3 levels are not associated with goiter . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study we show that fasted rat skin contains similar amounts of IGF 1 , IGFBP 3 and IGFBP 1 , although extract from fasted rat skin induced inhibition of collagen biosynthesis in cultured fibroblasts , compared to control rat skin extract . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the effects of short term transdermal estradiol on basal and exercise stimulated serum GH , IGF 1 , and associated binding proteins ( IGFBP 1 and IGFBP 3 ) in seven weight stable female amenorrheic athletes with percentage body fats greater that 12 % . ^^^ Serum samples for GH , IGF 1 , IGFBP 1 , and IGFBP 3 were obtained at baseline ( t 1 ) , 72 hr ( t 2 ) , 144 hr ( t 3 ) , and during three 90 minute trials of aerobic exercise . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum was analysed for free and total IGF 1 and 2 , free plus dissociable IGF 1 , IGFBP 1 , 2 and 3 , IGFBP 1 bound IGF 1 as well as IGFBP 3 proteolysis . ^^^ RESULTS : When compared with controls , diabetic patients ( n = 58 ) showed reduced ( P < 0 . 0005 ) levels of free and total IGFs , free plus dissociable IGF 1 and IGFBP 3 , whereas levels of IGFBP 1 , IGFBP 1 bound IGF 1 and IGFBP 2 were elevated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Northern blot , in situ hybridization and immunohistochemical technology were used to detect the expression of Cx43mRNA and its protein with MTT assay and silver colloid stain for the detection of cell proliferation , TUNEL method for determination of cell apoptosis , scrape loading dye transfer ( SLDT ) for GJIC , Western blot and immunohistochemical technology for bFGF , PDGF , EGFR , IGF 1 and IGFBP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To this end , we evaluated the behavior not only of IGF 1 but also of IGF 2 , IGF binding protein ( IGFBP ) 3 , and acid labile subunit , the subunits that constitute the main IGF complex in circulation ( 150 kDa complex ) , as well as of IGFBP 1 and IGFBP 2 , which are negatively regulated by GH and , like IGFBP 3 , able to influence the bioavailability of the IGFs . ^^^ The results demonstrated that the concentrations of IGF 1 ( 27 . 3 + / 2 . 0 nmol / liter ) , IGFBP 3 ( 66 . 9 + / 3 . 8 ) , and acid labile subunit ( 216 . 8 + / 13 . 6 ) in CTS affected subjects were not significantly different from those determined in controls ( 25 . 0 + / 2 . 9 , 74 . 4 + / 4 . 1 , and 241 . 0 + / 11 . 9 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of various doses of biosynthetic growth hormone on levels of IGF 1 binding protein 3 ( IGFBP 3 ) in patients with hypopituitarism and growth hormone deficiency ] . ^^^ OBJECTIVES : To study the effect of different doses of hGH ( biosynthetic human growth hormone Humatrope ) administration on the profile of IGFBP 3 in hypopituitary patients with GHD and to find out the extent of production of IGFBP 3 proteases , which results in the elevated biological IGF 1 activity . ^^^ METHODS : RIA ' s were used to analyse GH , IGF 1 , total IGFBP 3 and fIGF 1 . ^^^ RESULTS : Anabolic effect of administered hGH was demonstrated by significant increase of IGF 1 and total IGFBP 3 in both groups of patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The ability of the DNA PK phosphoform of IGFBP 3 to bind IGFs was severely attenuated , and in contrast to nonphosphorylated IGFBP 3 , the DNA PK phosphoform was unable to transport IGF 1 to the nucleus . ^^^ Furthermore , IGFBP 3 was phosphorylated by DNA PK when complexed to IGF 1 causing the phosphoform to release IGF 1 . ^^^ Together , these results suggest that when IGF 1 is cotransported into the nucleus by IGFBP 3 , phosphorylation of IGFBP 3 by nuclear DNA PK provides a means for releasing bound IGF 1 and creating a phosphoform of IGFBP 3 with increased affinity for nuclear components . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 was unaffected by 1 week NN 703 treatment , whereas serum IGFBP 3 was increased ( P < 0 . 05 vs . placebo ) also after correction for BMI . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to determine whether 4 months of intensified training would result in modified plasma insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) or IGFBP 3 in eight competitive cyclists and eight sedentary individuals and to define the relationships of these factors with glucose disposal . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Age was a strong determinant of both IGF 1 and IGFBP levels ; women aged 65 70 years had significantly lower IGF 1 and IGFBP 3 concentrations and significantly higher IGFBP 1 and IGFBP 2 concentrations than women aged 20 24 years . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the second series of experiments , the effects of single and multiple ( daily for 28 days ) injections of recombinant hIGF 1 ( 1 microg / animal ) on plasma IGF 1 , IGFBP 3 , T 3 , T 4 concentrations , the duration of pregnancy , and number of offspring delivered were assessed . ^^^ IGF 1 treatment caused an increase in plasma IGF 1 concentration , a reduction in plasma IGFBP 3 , T 3 , and T 4 concentrations , and a shorter duration of pregnancy but did not alter the number of pups delivered . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ribonuclease protection assays were used to measure steady state semimembranosus muscle and / or hepatic levels of IGF 1 , IGFBP 3 , IGFBP 5 , hepatocyte growth factor ( HGF ) , and myostatin messenger RNA ( mRNA ) in steers implanted from 32 to 38 d with Revalor S , a combined trenbolone acetate and estradiol implant . ^^^ Previous data from these same steers have shown that circulating IGF 1 and IGFBP 3 concentrations were 30 to 40 % higher ( P < 0 . 01 , n = 7 ) in implanted steers than in nonimplanted , control steers . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 in healthy pregnancies and patients with preeclampsia . ^^^ DESIGN AND METHODS : We studied serum levels of IGF 1 and IGFBP 3 in 68 pregnant women ; 28 mild preeclampsia , 13 severe preeclampsia , and 27 healthy pregnant control subjects . ^^^ CONCLUSIONS : IGF 1 and IGFBP 3 levels in patients with preeclampsia were compared with healthy pregnant control subjects . ^^^ IGF 1 and IGF I / IGFBP 3 ratio may be useful for estimate the severity of preeclampsia . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The authors evaluated the association between benign prostatic hyperplasia and IGF 1 and its binding protein IGFBP 3 in community dwelling men to determine whether this laboratory finding is manifest at the population level . ^^^ Urologic measures were assessed from the International Prostate Symptom Score , peak urinary flow rates , prostate volume , and serum prostate specific antigen ( PSA ) , and serum IGF 1 and IGFBP 3 levels were measured . ^^^ A low IGFBP 3 level was associated with an enlarged prostate ( odds ratio = 1 . 72 , 95 % CI : 1 . 05 , 2 . 82 ) , after simultaneous adjustment for IGF 1 and age , but not with other urologic measures . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGF ) are essential for normal growth and maintenance of lean muscle mass ; however , high insulin like growth factor 1 ( IGF 1 ) and low IGF binding protein 3 ( IGFBP 3 ) levels are also associated with several cancers . ^^^ Serum IGF 1 and IGFBP 3 were measured by ELISA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measuring IGF 1 , IGFBP 2 and IGFBP 3 from dried blood spots on filter paper is not only practical but also reliable . ^^^ OBJECTIVE : The aim of this study is to adapt measurements of IGF 1 , IGFBP 2 and IGFBP 3 to dried blood filter disk assays . ^^^ Recovery of IGF 1 , IGFBP 2 and IGFBP 3 in experiments using recombinant standards mixed with whole blood was well correlated with the recovery of the plasma fraction on the filter paper . ^^^ CONCLUSIONS : Taken together , these data clearly demonstrate that such a filter paper assay is a reliable procedure to monitor changes of IGF 1 , IGFBP 2 and IGFBP 3 content in blood . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Western immunoblot showed that type Ialpha collagen binds only IGFBP 3 but not IGF 1 , suggesting an IGF 1 independent mechanism of this interaction . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Expression of IGF 1 , IGFBP 3 and IGFBP 5 mRNA did not differ between CFP and MFP , suggesting that expression of these factors was not influenced by interactions between stroma and developing epithelium . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this study was to investigate serum levels of growth hormone ( GH ) , insulin growth factor 1 ( IGF 1 ) , and insulin growth factor binding protein 3 ( IGFBP 3 ) in 363 healthy caucasian men with and without decreased bone density , who had never experienced fractures . ^^^ Serum concentrations of GH , IGF 1 , and IGFBP 3 were measured using the immunofunctional ( GH ) and IRMA ( IGF 1 and IGFBP 3 ) methods . ^^^ Mean GH , IGF 1 , and IGFBP 3 levels were 0 . 2+ / 0 . 1 , 186 . 1+ / 177 . 3 , and 4990+ / 1460 ng / mL , respectively . ^^^ There were no significant differences between men with normal BMD and men with reduced BMD concerning GH , IGF 1 , and IGFBP 3 measurements . ^^^ In normal men ( 319 ) , mean GH , IGF 1 , and IGFBP 3 levels were 0 . 4+ / 0 . 1 , 192+ / 87 , and 4960+ / 1530 ng / mL , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A series of 1 benzoyl isoquinolines , based on compound 1 , was synthesized and evaluated for their ability to displace IGF 1 from its complex with IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Quinoline carboxylic acids are potent inhibitors that inhibit the binding of insulin like growth factor ( IGF ) to IGF binding proteins . 4 benzylquinolines 5 , based on a series of isoquinolines 1 , were prepared and tested as inhibitors of the IGF / IGFBP 3 complex based on their ability to displace IGF 1 from its binding to IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The concentrations of the following markers were determined in relevant serum samples : IGF 1 , IGFBP 3 , ALS , PIIINP , PINP , osteocalcin , and leptin . ^^^ The response of the IGFBP 3 to the hGH applications was lower in comparison to IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have undertaken a case control study among Greek women to explore the relation of major components of the IGF system ( IGF 1 , IGF 2 and IGF binding protein 3 , IGFBP 3 ) with endometrial cancer risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study included 29 pre pubertal children with obstructive adenotonsillar hypertrophy , and aimed to investigate the probable difference in energy intake and serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels before and 6 months after adenotonsillectomy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A cross sectional analysis of diet , IGF 1 and IGFBP 3 in healthy middle aged men . ^^^ Raised levels of IGF 1 and / or its molar ratio with IGFBP 3 were associated with higher intakes of milk , dairy products , calcium , carbohydrate and polyunsaturated fat ; lower levels with high vegetable consumption , particularly tomatoes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) , inhibitory to the mitogenic action of IGF 1 , was detected in epithelial cells of prostatic ventral lobes , but not in those of the dorsal , lateral or anterior lobes of IGF 1 transgenic mice . ^^^ IGF binding protein 3 ( IGFBP 3 ) , inhibitory to the mitogenic action of IGF 1 , was detected in epithelial cells of prostatic ventral lobes , but not in those of the dorsal , lateral or anterior lobes of IGF 1 transgenic mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The catabolic response was assessed by measuring total IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ CONCLUSION : In contrast to animal data , no differences in plasma IGF 1 or IGFBP 3 were seen in patients undergoing laparoscopic or conventional Nissen fundoplication . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We hypothesize that low IGF 1 and high IGFBP 3 levels might be associated with increased risk of ischemic heart disease ( IHD ) . ^^^ We measured IGF 1 and IGFBP 3 in serum from 231 individuals who had a diagnosis of IHD 7 . 63 years after blood sampling and among 374 control subjects matched for age , sex , and calendar time . ^^^ At baseline when all individuals were free of disease , subjects in the low IGF 1 quartile had significantly higher risk of IHD during the 15 year follow up period , with a relative risk ( RR ) of 1 . 94 ( 95 % CI , 1 . 03 to 3 . 66 ) of IHD compared with the high IGF 1 quartile group , when IGFBP 3 , body mass index , smoking , menopause , diabetes , and use of antihypertensives were controlled for . ^^^ Identification of a high risk population with low IGF 1 and high IGFBP 3 levels resulted in markedly higher risk of IHD ( RR 4 . 07 ; 95 % CI , 1 . 48 to 11 . 22 ) compared with the index group . ^^^ CONCLUSIONS : Individuals without IHD but with low circulating IGF 1 levels and high IGFBP 3 levels have significantly increased risk of developing IHD during a 15 year follow up period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We want to emphasise that in children with normal serum IGF 1 and IGFBP 3 levels accurate measurements of body proportions and skeletal radiographs in disproportionate cases are more important than reiterative GH stimulation tests , which prepubertally and in the early phase of puberty often show subnormal responses . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Despite GH hypersecretion , serum IGF 1 , IGFBP 3 , and ALS levels have all been reported to be low in patients with anorexia nervosa ( AN ) , while the degree of ternary complex formation in AN is unknown . ^^^ Following partial weight recovery , the 150 kD IGFBP 3 ternary complex was fully normalized , despite only partial normalization of serum GH and IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For IGF 1 , the adjusted OR ( 95 % CI ) of the top vs . bottom quartile was 1 . 1 ( 0 . 6 ; 2 . 1 ) ; for IGFBP 1 it was 0 . 7 ( 0 . 3 ; 1 . 3 ) ; for IGFBP 2 , 1 . 1 ( 0 . 5 ; 2 . 4 ) ; for IGFBP 3 , 1 . 6 ( 0 . 7 ; 3 . 5 ) , for C peptide , 1 . 3 ( 0 . 7 ; 2 . 7 ) and for IGF I / IGFBP 3 ratio , 1 . 0 ( 0 . 5 ; 1 . 8 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Optimal growth promoting doses differ by individual , and thus surrogate markers such as IGF 1 and IGFBP 3 levels are important in the assessment of GH treatment compliance , safety , efficacy , and overtreatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We characterized the GH IGF 1 axis by analyzing the individual components of the axis ( GH , IGF 1 , IGF binding protein 3 [ IGFBP 3 ] , acid labile subunit [ ALS ] ) and the corresponding ratios ( GH / IGF 1 , GH / IGFBP 3 , and GH / ALS ) and verified the links with circulating proinflammatory cytokines ( TNF alpha , IL 1beta , and IL 6 ) , in 34 patients with CLD and 12 healthy controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Equimolar NBP 3 + CBP 3 bound IGF 2 with an affinity ( 12 . 2 liter / nmol ) only 4 fold lower than that of the IGFBP 3 IGF 2 complex and IGF 1 with an affinity ( 3 . 2 liter / nmol ) 13 fold lower than IGFBP 3 IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 4 , IGFBP 5 , and nonglycosylated IGFBP 3 were shown to significantly enhance binding of IGF 1 to VN , whereas IGFBP 2 and glycosylated IGFBP 3 had a smaller effect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lung cancer risk is associated with higher plasma levels of insulin like growth factor 1 ( IGF 1 ) and / or lower levels of IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ( 1 nM ) enhanced release of IGFBP 3 while dexamethasone ( 1 nM ) diminished release of IGFBP 4 . ^^^ Furthermore , the specific stimulatory and inhibitory effects of IGF 1 and dexamethasone , respectively , on release of the predominant species of IGFBP 3 and IGFBP 4 , suggested that IGFBP production may be selectively modulated in a positive and negative manner . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and obesity , and in particular visceral obesity , are putative cancer risk factors . ^^^ Lack of association between adipose tissue distribution and IGF 1 and IGFBP 3 in men and women . ^^^ We investigated the relationship between adipose tissue distribution and IGF 1 and IGFBP 3 . ^^^ Insulin , IGF 1 , IGFBP 3 , and the ratio of IGF 1 : IGFBP 3 , measured by radioimmunoassay , were compared with age , body mass index , absolute and relative VAT and SQAT , and total abdominal fat . ^^^ In men and women , there was no correlation between IGF 1 , IGFBP 3 , or the ratio of IGF 1 : IGFBP 3 with body mass index , total fat , VAT or SQAT , or fasting insulin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously reported that the galactopoietic effect of LDPP is associated with an increase in circulating IGF 1 , which seems to occur independently of changes in concentrations of GH , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Estimation of IGF 1 and IGFBP 3 concentrations in serum in children with diabetes type 1 and growth disorders ] . ^^^ The aim of the study was to estimate the IGF 1 and IGFBP 3 levels in the group of children with diabetes type 1 and growth disturbances . ^^^ IGF 1 , IGFBP 3 , GH stimulation test , TSH , fT 4 and HbA 1 were determined . ^^^ In the studied group positive correlations between : IGF 1 and IGFBP 3 ; IGF 1 and BMI ; BMI and IGFBP 3 were determined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Twenty five pubertal HIV infected children were assessed for GH response ( GH AUC ( 0 120 min ) ) to arginine + GHRH testing , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , insulin , glucose , cholesterol , triglycerides , free fatty acids and nitric oxide levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The assessment of plasma levels of insulin like growth factor ( IGF 1 ) and binding protein ( IGFBP 3 ) during the maintenance therapy and after remissionof acute lymphoblastic leukemia ( ALL ) ] . ^^^ The aim of the study was the assessment of IGF 1 and IGFBP 3 plasma concentrations in children after intensive therapy of ALL completed according to age , weight , height and therapy protocols ( Methotrexate dose , Central Nervous System CNS prophylaxis ) . ^^^ We assessed plasma levels of IGF 1 and IGFBP 3 in radioimmunoassay method and the obtained results were related to mean age values ( SDScore ) . ^^^ After puberty ( Tanner 5 ) IGF 1 was 332 . 03+ / 75 . 66 ng / ml . b ) IGFBP 3 was highest after puberty ( 3837 . 4 ng / ml + / 598 . 49 ) ; before puberty it was 2899 . 68+ / 656 . 57 ng / ml , and in puberty it was 3593 . 14+ / 598 , 49 ng / ml . 2 . ^^^ There were no differences between IGF 1 SDS and IGFBP 3 SDS according to gender , prior CNS prophylaxis , methotrexate dose and phase of maintenance therapy and completed therapy . 5 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes of serum levels of osteocalcin , alkaline phosphatase , IGF 1 and IGF binding protein 3 during fracture healing . ^^^ In conclusion , serum markers such as osteocalcin , ALP , IGF 1 and IGFBP 3 reflected in part the osteoblastic activity during bone formation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
From indirect evidence , absorption of colostral IGF 1 and IGFBP 3 into systemic circulation may occur , but relative importance compared to endogenous sources is uncertain . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patients with obesity featured by decreased IGF 1 ( p < 0 . 05 ) , free IGF 1 ( p < 0 . 05 ) , IGFBP 1 ( p < 0 . 01 ) and IGFBP 3 ( p < 0 . 05 ) serum levels . ^^^ Type 1 diabetes was characterised by typical decrease in IGF 1 ( p < 0 . 05 ) , free IGF 1 ( p < 0 . 01 ) and IGFBP 3 ( p < 0 . 01 ) serum levels as well as by increase in IGFBP 1 ( p < 0 . 01 ) serum levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The reduction in free IGF 1 was paralleled by an increase in IGFBP 1 , an increase in the complex formation of IGFBP 1 and IGF 1 , and a modest reduction in IGFBP 3 proteolysis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In random normal human adult sera ( n = 37 ) , the median IGFBP rP 1 was 21 . 0 micro g / liter , and values did not correlate with levels of IGF 1 ( r = 0 . 085 , P = 0 . 61 ) , IGF 2 ( r = 0 . 051 , P = 0 . 75 ) , or IGFBP 3 ( r = 0 . 061 , P = 0 . 74 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Serum measures of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) that had been obtained before and after transplantation were reviewed ( with Institutional Review Board approval ) in a subset of pediatric ITx recipients . ^^^ Before transplantation , levels of IGF 1 and IGFBP 3 were low in 60 % and 67 % of patients , respectively . ^^^ Of the 3 patients who experienced negative linear growth velocity over time , 2 had low pretransplant levels of both IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum Sex Hormones , IGF 1 , and IGFBP 3 Exert a Sexually Dimorphic Effect on Lean Body Mass in Aging . ^^^ METHODS : Fasting serum IGF 1 , IGFBP 3 , testosterone , estrone , and sex hormone binding globulin were analyzed in 48 women on hormone replacement ( HRT ) ( unopposed oral estrogen , HRT+ , 74 . 0 + / 6 years ) , 135 women not on HRT ( HRT , 77 . 3 + / 7 years ) , and 128 healthy men ( men , ) . ^^^ LBM was greater , and IGF 1 , IGFBP 3 , and testosterone were lower in HRT+ versus HRT women ( p = . 02 , p = . 01 , p = . 04 , and , respectively ) . ^^^ LBM in men was positively related to IGF 1 ( p = . 02 ) and testosterone ( p < . 01 ) , whereas LBM was associated with IGFBP 3 ( p = . 04 ) and total fat ( p < . 001 ) in female HRT+ and total fat ( p < . 01 ) in HRT women . ^^^ IGFBP 3 decreased in all groups across age ( p < . 01 ) , and the ratio of IGF 1 to IGFBP 3 decreased faster in men compared to HRT+ and HRT women ( p = . 02 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Evaluation of growth hormone ( GH ) , insulin like growth factor ( IGF 1 ) and insulin like growth factor binding protein ( IGFBP 3 ) levels in youths with diabetes type 1 . 2 . ^^^ GH , IGF 1 and IGFBP 3 levels were estimated by use of radioimmunoenzymatic methods . ^^^ RESULTS : In adolescents with diabetes we found higher GH levels , and lower IGF 1 as well as IGFBP 3 levels in comparison to control group . ^^^ Statistically higher were IGF 1 and IGFBP 3 levels in children with diabetes duration longer than 10 years . ^^^ Metabolic control did not influence GH , IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thyroid hormones , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP 3 ) , selenium , zinc , and Phe blood levels were measured . ^^^ IGF 1 , IGFBP 3 , and thyroid hormone levels were within normal range . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No significant changes were noticed in the circulating levels of calcium ( Ca ) , parathyroid hormone ( iPTH ) , 25OHD , 24 , 25 ( OH ) 2D , 1 , 25 ( OH ) 2D , the insulin like growth factor 1 ( IGF 1 ) and its binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Significant decreases in IGF 1 , IGFBP 3 , IGF 1 : C ratio and triglyceride values and increases in uric acid and creatinine levels were noted . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) have each been associated with premenopausal breast cancer risks . ^^^ We analyzed data from a cross sectional study of 261 premenopausal Japanese women aged 20 54 yr with adequate nutritional status to evaluate the relationships between concentrations of IGF 1 and IGFBP 3 in serum and dietary intakes of soy , fats and other nutrients . ^^^ There was no significant correlation between soy product as well as soy isoflavone intake and serum IGF 1 or IGFBP 3 levels after controlling for age , total energy , percent body fat , and education level . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum osteocalcin , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , HbA ( 1c ) , and urine N telopeptides were measured . ^^^ IGF 1 , IGFBP 3 , osteocalcin , and N telopeptides were similar in diabetic subjects and control subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
They examined the expression of MMPs in cardiac tissues of rats infused with isoproterenol ( 3 mg / kg per day ) , the effect of a MMP inhibitor , SI 27 ( 5 mg / rat per day ) , on cardiac hypertrophy , and the expression of IGF 1 and IGFBP 3 . ^^^ MMP inhibition caused a regression in the myocyte hypertrophy in association with the suppression of both IGF 1 protein in myocytes and the degradation of IGFBP 3 protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Within a prospective cohort study , we measured testosterone , sex hormone binding globulin ( SHBG ) and IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 3 , insulin and leptin , in plasma from 149 cases and 298 controls . ^^^ Testosterone correlated positively with SHBG , whereas testosterone and SHBG correlated inversely with IGF 1 , IGFBP 3 , insulin , leptin and body mass index ( BMI ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Sixty seven breast surgical specimens from women with and without diabetes who did not have cancer and from women with and without diabetes who did have cancer were studied for insulin like growth factor 1 ( IGF 1 ) , the IGF 1 receptor ( IGF IR ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and oestrogen receptor 1 gene expression . ^^^ RESULTS : The expression of IGF 1 and IGF IR was significantly lower in the cancer groups , whereas there was no significant difference for IGFBP 3 between women with and without cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , IGF 1 and IGFBP 3 were significantly decreased ( IGF 1 : 0 . 3+ / 1 . 5 vs 0 . 7 + / 1 . 2 SDS , P=0 . 001 ; IGFBP 3 : 0 . 3+ / 1 . 1 vs 1 . 0+ / 1 . 1 SDS , P=0 . 02 ) . ^^^ Children with AML showed significantly higher IGFBP 2 ( P=0 . 04 ) and significantly lower IGF 1 ( P=0 . 03 ) and IGFBP 3 ( P=0 . 05 ) levels than children with ALL at day 100 after HSCT . ^^^ Increased IGFBP 2 and decreased IGF 1 and IGFBP 3 may be associated with the increased proliferation rate of transplanted bone marrow . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After six month treatment with recombinant human growth hormone ( rhGH ) , serum level of IGFBP 3 in 59 GHD significantly increased from 1 . 3 + / 0 . 7 mg / L to 2 . 7 + / 0 . 9 mg / L , accompanied by an increase of body heights , growth velocities and serum level of IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The loss of insulin like growth factor 1 ( IGF 1 ) and IGFBP 3 found in nephrotic children may directly prompt growth defect and retardation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , and its binding protein 3 ( IGFBP 3 ) correlated with fetal development ] . ^^^ OBJECTIVE : To study the relations between IGF 1 , IGFBP 3 and human fetal growth . ^^^ METHODS : The blood samples of maternal serum ( MS ) and umbilical cord serum ( UCS ) from 81 cases with singleton term pregnancy including 38 cases of normal pregnancy ( NP ) , 20 cases of gestational diabetic mellitus ( GDM ) , 23 cases of macrosomia of non diabetic pregnancy ( MNDP ) were examined for IGF 1 and IGFBP 3 levels by immunoassay kit from DSL , USA . ^^^ IGF 1 and IGFBP 3 of UCS in these three groups were significantly lower than that of MS ( P < 0 . 001 ) . ^^^ IGF 1 and IGFBP 3 play important roles for human fetal growth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The molarities of plasma 41 kDa IGFBP and total IGF 1 were comparable , and they were positively correlated , suggesting that salmon 41 kDa IGFBP is a main carrier of circulating IGF 1 in salmon , as is mammalian IGFBP 3 in mammals . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 circulates in free , ternary [ IGF 1 + IGFBP 3 + acid labile subunit ( ALS ) ] , and binary ( IGF 1 + IGFBP ) molecular complexes , and the relative proportions regulate IGF 1 extravascular shifting and bioavailability . ^^^ Components of the IGF 1 system ( total and free IGF 1 ; IGFBP 1 , 3 , and ALS ; nonternary IGF 1 and IGFBP 3 ) , biomarkers of metabolic and nutritional status ( transferrin , ferritin , prealbumin , glucose , free fatty acids , glycerol , beta hydroxybutyrate ) , and body composition were measured in 12 men ( 22 + / 3 yr , 87 + / 8 kg , 183 + / 7 cm , 20 + / 5 % body fat ) on days 1 , 3 , and 4 during a control and experimental ( Exp ) period . ^^^ IGF 1 and IGFBP 3 were measured by immunoassay before and after immunoaffinity depletion of ALS based complexes ( i . e . , ternary complex removal ) . ^^^ Exp produced losses in body mass ( 3 . 0 % ) , lowered total IGF 1 ( 24 % ) , free IGF 1 ( 42 % ) , IGFBP 3 ( 6 % ) , nonternary IGF 1 ( 27 % ) , and IGFBP 3 ( 16 % ) , and increased IGFBP 1 ( 256 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied the plasma concentrations of IGF 1 , IGF 2 , IGF binding protein 3 ( BP 3 ) , and GH in cord blood taken from the offspring of 1650 singleton Caucasian pregnancies born at term and related these values to birth weight , length , and head circumference . ^^^ Cord plasma concentrations of IGF 1 , IGF 2 , and IGFBP 3 were influenced by factors influencing birth size : gestational age at delivery , mode of delivery , maternal height , and parity of the mother . ^^^ Plasma GH concentrations were inversely related to the plasma concentrations of IGF 1 and IGFBP 3 ; 10 . 2 % of the variability in cord plasma IGF 1 concentration and 2 . 7 % for IGFBP 3 was explained by sex of the offspring and parity . ^^^ Mean cord plasma concentrations of IGF 1 ( males , 66 . 4 + / 1 . 2 micro g / liter ; females , 74 . 5 + / 1 . 3 micro g / liter ; P < 0 . 001 ) and IGFBP 3 ( males , 910 + / 13 micro g / liter ; females 978 + / 13 micro g / liter ; P < 0 . 001 ) were significantly lower in males than females . ^^^ After adjustment for gestational age , parity , and maternal height , cord plasma concentrations of IGF 1 and IGFBP 3 along with sex explained 38 . 0 % of the variability in birth weight , 25 . 0 % in birth length , and 22 . 7 % in head circumference . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
End points included changes in fasting insulin , glucose , insulin resistance , IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , and IGF 1 : IGFBP 3 molar ratio between baseline and week 15 . ^^^ However , significant differences between groups were observed for changes in IGF 1 ( 7 . 4 ng / ml ; P = 0 . 045 ) , IGFBP 3 ( +180 . 5 ng / ml ; P = 0 . 021 ) , and IGF 1 : IGFBP 3 molar ratio ( 0 . 006 ; P = 0 . 017 ) . ^^^ Exercise training had significant physiological effects on IGF 1 , IGFBP 3 , and IGF 1 : IGFBP 3 molar ratio in postmenopausal breast cancer survivors . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Radioimmunometrically measured IGF 1 and IGFBP 3 concentrations were available for 312 men and 326 postmenopausal women ages 50 years or older . ^^^ Mean IGF 1 concentrations were 144 ng / ml and 121 ng / ml for men and women , respectively ( gender difference , P < 0 . 0001 ) , and mean IGFBP 3 concentrations were 3710 ng / ml and 4147 ng / ml for men and women , respectively ( gender difference , P < 0 . 0001 ) . ^^^ IGF 1 and IGFBP 3 decreased with age ( P for trend < 0 . 0001 ) ; the age related decrease in the IGF 1 : IGFBP 3 molar ratio was stronger in women than men . ^^^ Intake of calcium from food and supplement was associated positively with circulating IGF 1 , IGFBP 3 , and molar ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 , IGF 2 and IGFBP 3 significantly decreased , and those of GHBP slightly increased with age from 20 to 92 years ( n=327 r= 0 . 64 p < 0 . 0001 ; n=45 r= 0 . 44 p < 0 . 003 ; n=91 r= 0 . 23 p < 0 . 03 and n=61 r=0 . 26 ; p < 0 . 05 respectively ) . ^^^ Our results suggest that the age related decline in plasma levels of IGF 1 , IGF 2 and IGFBP 3 occurs independently from malnutrition and inflammation processes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have examined whether pre and postnatal growth patterns have a long term influence on adult IGF 1 and IGFBP 3 levels . ^^^ MEASUREMENTS : Anthopometric measures at birth , postnatally up to 5 years of age and in adulthood , and serum measures of IGF 1 and IGF 1 to IGFBP 3 ratio at mean age of 25 years . ^^^ Adiposity was inversely associated with the IGF 1 and IGF 1 to IGFBP 3 ratio and positively associated with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For 943 of these women , we had previously measured IGF 1 and IGFBP 3 plasma levels , and for 861 of these subjects , plasma retinol levels were available . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Within the framework of a large ( 769 patients ) , randomised double blind clinical trial comparing exemestane ( EXE ) with megestrol acetate ( MA ) , serum 17 beta estradiol ( E 2 ) , estrone ( E 1 ) , estrone sulphate ( E1S ) , bone alkaline phosphatase ( BAP ) , carboxy terminal cross linking telopeptide of type 1 collagen ( ICTP ) and the components of insulin like growth factor ( IGF ) family ( IGF 1 , IGF 2 and IGFBP 3 ) were determined in 53 patients ( 24 randomised to EXE and 29 ramdomised to MA ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of this study is to evaluate the influence of three different HRT regimens on serum levels of SHBG , IGF 1 , IGFBP 1 and IGFBP 3 in postmenopausal women . ^^^ Levels of SHBG , IGF 1 , IGFBP 1 and IGFBP 3 in the serum were measured by means of a specific immunoassay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The significantly reduced levels of IGF 1 ( p < 0 . 001 ) , IGF 2 ( p=0 . 017 ) and IGFBP 3 and the increased presence of IGFBP 2 were found in patients with echinococcosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A low protein intake decreased the circulating levels of IGF 1 , IGFBP 3 , GH and insulin whereas the serum levels of IGFBP 1 were increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The assessment ( IGF 1 , IGFBP 3 , lipid profile , body composition , glycated hemoglobin , oral glucose tolerance test , ISI HOMA and ISI composite derived from OGTT ) was carried out before GH and every 3 months during the first year of treatment , and then every 6 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels were significantly reduced , but by different degrees , in both mouse lines , but IGFBP 1 and 4 levels were reduced and IGFBP 2 levels increased in GHR / mice but unaltered in GHA mice . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 inhibited both IGFBP 3 binding to membranes and phosphorylation . ^^^ However , incubation of T 47D cells with IGFBP 3 enhanced binding of ( 125 ) 1 IGF 1 to the cell monolayer indicating that membrane bound IGFBP 3 was able to bind IGF 1 . ^^^ Immobilized IGFBP 3 when phosphorylated by T 47D membranes bound significantly more ( 125 ) 1 IGF 1 than nonphosphorylated IGFBP 3 . ^^^ Treatment with alkaline phosphatase significantly reduced ( 125 ) 1 IGF 1 binding to phosphorylated immobilized IGFBP 3 and also reduced ( 125 ) 1 IGF 1 to T 47D cell monolayers preincubated with IGFBP 3 . ^^^ These data demonstrate that binding of IGFBP 3 to breast cancer membranes is accompanied by phosphorylation at the plasma membrane and that both processes are inhibited by IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recent reports suggest that colorectal cancer is positively related to insulin like growth factor 1 ( IGF 1 ) and inversely related to insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ These patients ' stored sera and those of 282 age matched controls were measured for IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We found a significant decrease between the 21 30 year age group and the > 70 year age group for total testosterone ( 42 . 4 % ) , FAI ( 65 . 5 % ) , cFT ( 60 . 0 % ) , % cFT ( 30 . 0 % ) , DHEAS ( 71 . 9 % ) , E 2 ( 35 . 4 % ) , TSH ( 23 . 6 % ) , IGF 1 ( 40 . 3 % ) and IGFBP 3 ( 26 . 5 % ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Leptin , VEGF , IGF 1 , and IGFBP 3 concentrations in serum and follicular fluid of women undergoing in vitro fertilization . ^^^ We studied the influence of recombinant follicle stimulating hormone ( rFSH ) stimulation on the concentration of leptin , vascular endothelial growth factor ( VEGF ) , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in serum and follicular fluid ( FF ) in women undergoing assisted reproduction . ^^^ The follicular fluid ( FF ) obtained during oocyte retrieval was collected and the concentration of leptin , VEGF , IGF 1 and IGFBP 3 were measured in all samples . ^^^ Serum concentrations of VEGF , IGF 1 and IGFBP 3 significantly decreased during rFSH treatment . ^^^ A strong correlation was found between the FF levels of IGF 1 , IGFBP 3 , leptin and respective serum levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 measurements on filter paper blood spots in children and adolescents on GH treatment : use in monitoring and as markers of growth performance . ^^^ METHODS : IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were measured in ELISAs , using dried capillary blood collected onto filter papers . ^^^ RESULTS : IGF 1 and IGFBP 3 SDS were higher in the TS and non GHD groups ( mean +0 . 01 and +0 . 1 respectively ) than in those with GHD ( mean value 0 . 6 ) . ^^^ Nineteen per cent of the IGF 1 values ( 13 % low , 6 % high ) and 12 % of IGFBP 3 values were abnormal ( 10 % low , 2 % high ) . ^^^ CONCLUSIONS : From this cross sectional study of IGF monitoring across a broad range of diagnoses and ages , it can be concluded that the majority of children on GH have normal levels of IGF 1 and IGFBP 3 , but 26 % of tests could suggest that a change of GH dose should be considered . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , the relationship between carotid atherosclerosis and serum levels of IGF 1 and its binding protein 3 ( IGFBP 3 ) in patients with essential hypertension has not been established . ^^^ METHODS : The carotid IMT , blood pressure ( BP ) , serum lipid profiles , and serum IGF 1 and IGFBP 3 contents were determined in 54 hypertensive patients ( 19 with and 35 without carotid plaque ) and 52 normotensive controls without plaque . ^^^ Hypertensive patients with plaque , compared with those without plaque or the normotensive controls , had the highest values of carotid IMT , LDL cholesterol , IGF 1 , and IGFBP 3 . ^^^ Multiple logistic regression analysis revealed that the IGFBP 3 level was associated with a ninefold ( 95 % confidence interval 2 . 6 31 ) higher risk of carotid plaque formation compared with LDL cholesterol or IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Premature thelarche ( PT ) is characterised by precocious breast development without any other sign of puberty , normal height velocity ( HV ) and normal bone maturation , while girls with central precocious puberty ( CPP ) show increased HV , bone maturation and increased serum IGF 1 and IGFBP 3 levels . ^^^ This prompted us to study serum IGF 1 and IGFBP 3 concentrations in girls with PT . ^^^ Serum IGF 1 and IGFBP 3 were determined in all girls by IRMA . ^^^ In conclusion , IGF 1 and IGF I / IGFBP 3 values in PT are intermediate between those observed in prepubertal children and in CPP , suggesting that PT could be a very early stage of puberty with slight but real changes in the GH IGF axis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this case control study , we analyzed the relationship between serum levels of IGF 1 and IGFBP 3 , and SILs of the cervix . ^^^ Case patients with either high grade or low grade SILs had significantly higher serum levels of IGF 1 , IGFBP 3 , and molar ratios of IGF 1 : IGFBP 3 than the control subjects did . ^^^ IGF 1 levels in the highest quartile were associated with significantly higher risk of SILs compared with the lowest quartile , independent of IGFBP 3 levels . ^^^ The odds ratio for the fourth quartile of IGF 1 level , relative to the first quartile , was 8 . 54 ( 95 % confidence interval , 4 . 15 17 . 60 ; P < 0 . 0001 ) after adjustment for age , ethnicity , smoking status , and IGFBP 3 level . ^^^ However , after adjustment for IGF 1 , no relationship was evident between IGFBP 3 level and risk of SILs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neither binding of IGF 1 to IGFBP 3 nor binding of latency associated protein ( LAP ) with LTBP 1 inhibited the interaction of IGFBP 3 with LTBP 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Overall , GH treatment increased plasma GH , IGF 1 ( both P < 0 . 001 ) , and IGFBP 3 ( P < 0 . 02 ) ; however , GH treatment increased total IGF 1 , free IGF 1 , and IGFBP 3 , and decreased IGFBP 2 mainly at the high feeding level ( GH 10 feeding level interaction ; P < 0 . 02 , 0 . 01 , 0 . 03 , and 0 . 10 , respectively ) . ^^^ The high feeding level increased insulin , free and total IGF 1 , and IGFBP 3 ( all P < 0 . 001 ) , but decreased GH and IGFBP 2 ( both P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For Experiment 2 , CLs ( from Experiment 1 ) were enzyme dissociated and luteal cells cultured ( 24 h ) in Medium 199 ( M 199 ) containing ( 0 500 ng / ml ) IGFBP 3 ( + / IGF 1 ; 100 ng / ml ) , or ( 0 10 microg / ml ) IGFBP 3 AB . ^^^ In Experiment 2 , IGFBP 3 ( w IGF ) inhibited the steroidogenic actions of IGF 1 , but had no significant actions alone ( IGFBP 3 w / o IGF ) . ^^^ We conclude that IGFBP 3 inhibits IGF 1 actions in the porcine CL . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NO plays a role in LPS induced decreases in circulating IGF 1 and IGFBP 3 and their gene expression in the liver . ^^^ In this study , we administered aminoguanidine , a relatively selective inducible nitric oxide synthase ( iNOS ) inhibitor , to study the role of nitric oxide ( NO ) in LPS induced decrease in IGF 1 and IGFBP 3 . ^^^ These data suggest that iNOS plays an important role in LPS induced decrease in circulating IGF 1 and IGFBP 3 by reducing IGF 1 and IGFBP 3 gene expression in the liver . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The statistical significance of this association was lost after further adjustment for serum growth factors [ growth hormone , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) ] , hormones [ follicle stimulating hormone ( FSH ) and progesterone ] , and body size ( body mass index and waist : hip ratio ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , and IGF binding protein 3 were inversely associated with the presence of neonatal jaundice and blood creatinine , after controlling for blood protein levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of dehydroepiandrosterone sulfate ( DHEA S ) , total testosterone , free testosterone , 17beta estradiol , cortisol , human growth hormone ( hGH ) , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) were determined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , no association in risk was observed with increasing levels of IGF 1 or IGF binding protein 3 ( IGFBP 3 ) , before or after adjustment these factors . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin resistance in the liver specific IGF 1 gene deleted mouse is abrogated by deletion of the acid labile subunit of the IGF binding protein 3 complex : relative roles of growth hormone and IGF 1 in insulin resistance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Specifically , hyperinsulinemia elevates serum concentrations of free insulin like growth factor 1 ( IGF 1 ) and androgens , while simultaneously reducing insulin like growth factor binding protein 3 ( IGFBP 3 ) and sex hormone binding globulin ( SHBG ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Endotoxin at low doses stimulates pituitary GH whereas it decreases IGF 1 and IGF binding protein 3 in rats . ^^^ While it is well known that sepsis inhibits serum IGF 1 and its gene expression in the liver , the effect on pituitary GH and IGF binding protein 3 ( IGFBP 3 ) is poorly understood . ^^^ The GH IGF 1 IGFBP 3 response to different doses of lipopolysaccharide ( LPS ) administration has been investigated in adult male rats . ^^^ These data suggest a biphasic LPS effect on pituitary GH , a stimulatory effect at low doses and an inhibitory effect at higher doses , whereas it has a clear inhibitory effect on IGF 1 and IGFBP 3 synthesis in the liver . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present studies on 48 kidney transplant recipients and 25 healthy volunteers included concentrations of parathormone , osteocalcin , bone specific alkaline phosphatase , serum CrossLaps , calcidiol , calcitriol , ICTP , PICP , tartrate resistant acid phosphatase , beta 2 microglobulin , IGF 1 , IFGBP 1 , IGFBP 3 , OPG , and RANKL using commercially available kits for measurements . ^^^ OPD was related to age , time on dialysis prior transplantation , urea , platelet count , CSA dose , azathioprine dose , 25 ( OH ) D ( 3 ) , TRAP , IGF 1 , IGFBP 3 , whereas RANKL was related to leukocyte count , CSA concentration and dose , urine DPD , and beta 2 microglobulin content . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : In 50 ( 39 males , 11 females ) histologically confirmed and TNM graded patients with HCC who had not received adjuvant chemotherapy , the basal serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and alpha fetoprotein ( AFP ) were measured . ^^^ RESULTS : The levels of IGF 1 , IGF 2 and IGFBP 3 were diminished , as is the case when nutrition , hepatic function and growth hormone ( GH ) secretion are decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In any tumors that arose in animals injected with the IGFBP 3 expressing cells , there was a complete loss of IGFBP 3 activity , as measured by binding to IGF 1 and IGF 2 proteins . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Oral estradiol reduced baseline ( P < 0 . 05 ) and GH stimulated ( P < 0 . 05 ) IGF 1 levels , and GH stimulated IGF binding protein 3 ( IGFBP 3 ) levels ( P < 0 . 05 ) . ^^^ High dose transdermal estrogen did not affect basal levels of IGF 1 or IGFBP 3 , but reduced the response of these GH dependent peptides to GH stimulation ( P < 0 . 05 ) . ^^^ Low dose transdermal estrogen did not alter either baseline or peak IGF 1 levels , but reduced the peak IGFBP 3 response to GH stimulation ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We critically evaluated the diagnostic value of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) in GH deficiency ( GHD ) in children and adults using receiver operating characteristic ( ROC ) plot analysis . ^^^ The SD score ( SDS ) for IGF 1 was calculated from 596 normal subjects ( 212 children and 384 adults ) , and the SDS for IGFBP 3 was calculated from 350 normal subjects ( 212 children and 138 adults ) . ^^^ An accurate diagnosis was obtained using IGF 1 SDS alone in GHD children 65 % ; ISS , 97 % ; AGHD CO , 92 % ; AGHD AO , 86 % , with IGFBP 3 SDS alone in GHD children 60 % ; ISS , 90 % ; AGHD CO , 75 % ; AGHD AO , 68 % . ^^^ IGF 1 proved to be more useful because of its good diagnostic efficiency and accuracy in both children and adults , whereas IGFBP 3 did not significantly contribute to the diagnosis of GHD . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Some of them , like IGF 1 or IGF binding protein 3 or 5 , may play an important role in the development of thyroid nodules through paracrine mechanisms . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Nuclear localization of exogenously added IGFBP 3 was rapid , occurring within 15 min , inhibited by co incubation and extracellular sequestration with IGF 1 , and dependent on the transferrin binding C terminal peptide region of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A synergistic effect on breast cancer risk was suggested for IGF 1 or IGFBP 3 with estrone or testosterone among both pre and postmenopausal women . ^^^ Similar patterns of association were also seen for IGF 1 with testosterone as well as for IGFBP 3 with estrone or testosterone , and some of the interactions were statistically significant or borderline significant ( P < 0 . 10 ) . ^^^ No joint effects were found for IGF 1 or IGFBP 3 with estradiol or estrone sulfate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We determined whether IGF 1 and IGFBP 3 predict second breast cancer risk , and whether their changes during fenretinide explain observed reductions in second breast cancer in women < / =50 years of age . ^^^ EXPERIMENTAL DESIGN : Within a Phase 3 trial , we measured baseline and 1 year levels of IGF 1 , IGFBP 3 , and their ratio in 302 subjects on fenretinide and 220 controls who provided plasma samples . ^^^ The prognostic effect of IGF 1 and IGFBP 3 , and the surrogate effect of IGF 1 during fenretinide were assessed by Cox models after 9 . 4 years . ^^^ RESULTS : Among controls , high IGF 1 and low IGFBP 3 were associated with elevated second breast cancer risk [ top versus bottom tertile , IGF 1 , hazard ratio ( HR ) = 1 . 94 , 95 % confidence interval ( CI ) , 0 . 87 4 . 31 , P = 0 . 105 ; and IGFBP 3 , HR = 0 . 40 , 95 % CI , 0 . 18 0 . 93 , P = 0 . 033 ] . ^^^ Fenretinide induced reductions of IGF 1 , IGFBP 3 , and IGF 1 : IGFBP 3 of 8 % ( 95 % CI , 2 12 % ; P = 0 . 004 ) , 3 % ( 95 % CI , 1 5 % ; P = 0 . 002 ) , and 5 % ( 95 % CI , 0 10 % ; P = 0 . 050 ) , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Liver biochemistry ( serum albumin , alkaline phosphatase , lactic dehydrogenase , aspartate aminotransferase [ AST ] , and bilirubin ) , total insulin like growth factor 1 ( IGF 1 ) , and insulin growth factor binding protein 3 ( IGFBP 3 ) were determined at all ages . ^^^ Alkaline phosphatase , IGF 1 , IGFBP 3 , and lactic dehydrogenase levels did not differ between the feeding groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We performed a population based case control study in Sweden , including 288 endometrial cancer patients and 392 control women and analysed total serum IGF 1 , IGFBP 1 , IGFBP 3 , insulin and BMI levels stratified by disease and hormone replacement therapy status ( HRT ) . ^^^ There were no trends of increasing risk according to quartiles of IGF 1 , IGFBP 1 , IGFBP 3 and insulin serum levels . ^^^ Serum IGF 1 , IGFBP 1 , IGFBP 3 and insulin levels seem unrelated to endometrial cancer risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 is largely bound to IGF binding protein 3 ( IGFBP 3 ) , which can inhibit IGF 1 action and enhance delivery of IGF 1 to tissues . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Treatment with IGF BP 3 significantly reduced the levels ( mean intensity per pixel ) of EGF R , IGF 2 , and VEGF in all three cell lines and IGF 1 receptor ( IGF IR ) in representative ME 180 cell line . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our study investigated the association of breast cancer risk as assessed by mammographic density with insulin like growth factor 1 ( IGF 1 ) and one of its binding proteins ( IGFBP 3 ) in healthy premenopausal women with different ethnic backgrounds . ^^^ Serum concentrations of IGF 1 and IGFBP 3 were measured by double antibody ELISA . ^^^ We calculated Spearman correlation coefficients between mammographic characteristics and analytes and estimated means of mammographic characteristics by quartiles of IGF 1 and IGFBP 3 while adjusting for age , body mass index ( BMI ) and reproductive factors . ^^^ In this group of 240 women , IGF 1 , IGFBP 3 and percent densities did not differ significantly by ethnicity . ^^^ Whereas mammographic densities were not associated with IGF 1 , we found an inverse relation with IGFBP 3 ( r ( s ) = 0 . 15 , p = 0 . 02 ) and a positive association with the IGF I / IGFBP 3 ratio ( r ( s ) = 0 . 13 , p = 0 . 04 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We used a muscle biopsy technique in conjunction with real time PCR analysis to examine the time course of changes in muscle IGF 1 , IGFBP 3 , myostatin , and hepatocyte growth factor ( HGF ) mRNA in the longissimus muscles of Revalor S implanted and nonimplanted steers on d 0 , 7 , 12 , and 26 after implantation ( nine steers / treatment group ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of ST , IGF 1 , IGFBP 2 , and IGFBP 3 were determined . ^^^ Average concentrations of ST , IGF 1 , and IGFBP 3 were greater in males than females ( P < 0 . 01 ) . ^^^ Concentrations of IGF 1 and IGFBP 3 increased in males and females ( P < 0 . 01 ) , and concentrations of IGF 1 began to plateau at approximately the same time as growth rate differences were observed ( 16 wk of age ) . ^^^ Correlations between average daily gain , ST , IGF 1 , IGFBP 2 , and IGFBP 3 were determined . ^^^ Concentrations of IGFBP 2 were negatively correlated ( P < 0 . 01 ) with IGF 1 and positively correlated ( P < 0 . 01 ) with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Anthropometric data , IGF 1 and IGF binding protein 3 levels , glycemia and insulinemia , lipid profile , and body composition using dual 10 ray absorptiometry and abdominal computed tomography scan were recorded at completion of GH treatment and 1 yr later . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured height , weight , and lower leg length ( LLL ; n = 10 ) and markers of bone formation [ bone alkaline phosphatase ( BALP ) and C terminal propeptide of type 1 collagen ( P1CP ) ] , bone resorption [ C terminal telopeptide of type 1 collagen ( 1CTP ) ] , soft tissue collagen turnover [ N terminal propeptide of type 3 procollagen ( P3NP ) ] , and the GH axis [ IGF 1 and its binding proteins ( IGFBP 3 and IGFBP 2 ) ] before and after each course ( n = 25 ) and on completion of treatment ( n = 12 ) . ^^^ Precourse levels of BALP , P1CP , 1CTP , P3NP , IGF 1 , and IGFBP 3 showed an upward trend during treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND / AIM : Insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) generation tests are both sensitive and specific measures of growth hormone ( GH ) sensitivity . ^^^ We report our analysis of the correlation of low and high dose GH IGF 1 and IGFBP 3 generation tests among patients with GH deficiency , GH insensitivity , and idiopathic short stature . ^^^ RESULTS : The serum concentrations of IGF 1 and IGFBP 3 correlated significantly from one test to the other , regardless of the diagnosis . ^^^ In normal subjects and patients with GH insensitivity and GH deficiency , the delta over baseline in IGF 1 and IGFBP 3 in the low dose test was highly predictive of the delta values in the high dose test . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have prospectively investigated maternal serum levels of IGF 1 , IGF 2 , and the main binding protein insulin like growth factor binding protein 3 ( IGFBP 3 ) in women with and without preeclampsia . ^^^ RESULTS : There were no significant differences in serum concentrations IGF 1 and IGFBP 3 in the 17th or the 33rd week of gestation between women who developed preeclampsia or not . ^^^ CONCLUSION : In women who developed preeclampsia , we found no support for the hypothesis that the disease was preceded by lower serum levels of IGF 1 and IGF 2 , or higher serum levels of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood was drawn before biopsy , serum extracted and stored frozen until IGF 1 and IGFBP 3 were measured . ^^^ Serum IGF 1 did not correlate with the number of positive biopsies , with means of 176 . 7 , 178 . 3 and 164 . 4 ng / mL , respectively ( P = 0 . 3 ) , while the mean IGFBP 3 was 2695 , 2795 and 2572 ng / mL , respectively ( P = 0 . 09 ) . ^^^ The additive percentiles of tumour volume in positive biopsies were assessed for each patient but serum IGF 1 and IGFBP 3 did not correlate ( P = 0 . 7 and 0 . 9 , respectively ) . ^^^ In all , 92 patients had a Gleason score of < 7 , 80 a score of 7 and 69 a score of > 7 ; the mean ( sd ) IGF 1 levels for the three groups were 181 ( 39 ) , 174 . 6 ( 35 ) and 176 ( 26 ) ng / mL , and the mean IGFBP 3 2798 ( 240 ) , 2735 ( 284 ) and 2647 ( 221 ) ng / mL , respectively , none of the differences being statistically significant . ^^^ CONCLUSIONS : Serum IGF 1 and IGFBP 3 do not correlate with quantity of cancer or Gleason score in biopsy samples from patients with prostate cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) appear to influence the growth of breast cancer cells in vitro , and epidemiological studies suggest higher serum IGF 1 levels increase the risk of breast cancer . ^^^ IGF 1 and IGFBP 3 have therefore been measured in women with advanced breast cancer to determine if changes in serum levels predict the response to treatment by chemotherapy . ^^^ METHODS : Serum IGF 1 and IGFBP 3 levels were measured in 14 patients before and after 1 week of chemotherapy . ^^^ RESULTS : Mean basal serum levels of IGF 1 and IGFBP 3 were not significantly different between patients with advanced breast cancer and controls or women with early breast cancer . ^^^ Patient survival was not significantly related to baseline IGF 1 or IGFBP 3 levels , but when the fall in serum levels 1 week after starting treatment was expressed either as absolute change or as a percentage of baseline , those individuals with a decrease in IGFBP 3 greater than the median had significantly poorer survival ( median survival 5 . 5 months vs 18 months ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN : Cross sectional study in 121 healthy children ( 65 male , 56 female ) aged 5 18 years , in whom height , weight , body mass index ( BMI ) , pubertal status and measurements of IGF 1 , IGFBP 3 , IGFBP 1 and leptin were available . ^^^ Ghrelin was also negatively correlated to IGF 1 ( rs = 0 . 48 , P < 0 . 001 ) , IGFBP 3 ( rs = 0 . 32 , P < 0 . 001 ) and leptin ( rs = 0 . 22 , P = 0 . 02 ) but not IGF 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Administration of IGF 1 either alone or as an IGF I / IGFBP 3 complex reduced the severity of insulitis and delayed the onset of T1D in nonobese diabetic mice , but IGF I / IGFBP 3 was significantly more effective . ^^^ Thus , IGF I / IGFBP 3 affords more efficient protection from insulitis , beta cell destruction , and T1D than IGF 1 , and this complex may represent an efficacious therapeutic treatment for the prevention of T1D . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 concentrations were lower both the first and second morning after the last injection during the period with three injections per week . ^^^ These results suggest that the same weekly dose of GH given as three injections per week reduces serum IGF 1 and IGFBP 3 levels without affecting Lp ( a ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Determination of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in serum and plasma : comparisons using the Bland Altman method . ^^^ Blood was obtained from 47 healthy volunteers ( age 21 72 years ) in serum , heparin plasma and EDTA plasma , and IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 measured , and results compared . ^^^ Mean values for IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 determined in all three media were generally comparable ; correlations were generally strong and significant ( P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The peritoneal fluid of women with endometriosis contains an increased insulin like growth factor 1 ( IGF 1 ) bioavailability , which is produced by limited hydrolysis of urokinase type plasminogen activator ( uPA ) on IGF binding protein 3 ( IGFBP 3 ) . ^^^ These data possibly explain the origin of the increased content of uPA activity , IGF 1 bioavailability , and NH ( 2 ) truncated forms of IGFBP 3 in the peritoneal fluid of women with endometriosis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Influence of acute exercise on urinary protein , creatinine , insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 concentrations in children . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the major form of binding protein in human circulation and functions as a carrier for IGF 1 . ^^^ Our goal was to determine the effects of volleyball exercise on the concentrations of urine protein , creatinine , IGF 1 , and IGFBP 3 in children and to find out whether these effects differ between boys and girls . ^^^ Urinary protein , creatinine , IGF 1 and IGFBP 3 levels were analysed . ^^^ Urinary protein , creatinine and IGF 1 concentrations were increased after two hours of exercise wheres urinary IGFBP 3 concentrations did not change . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dexamethasone treatment decreased ( P < 0 . 05 ) plasma IGF binding protein ( IGFBP ) 1 on days 7 and 14 , but increased ( P < 0 . 05 ) plasma IGFBP 1 , decreased ( P < 0 . 05 ) plasma IGF 1 and IGFBP 3 , and decreased hepatic mRNA for growth hormone receptor ( GHR ) and IGF 1 on day 42 . ^^^ The combined dexamethasone and growth hormone treatment increased plasma growth hormone concentrations on day 7 and resulted in the highest plasma concentrations of IGF 1 and IGFBP 3 ( day 7 to day 28 ) as well as the greatest abundance of hepatic GHR ( day 14 ) and IGF 1 ( days 7 and 14 ) mRNA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , specimens collected at intervals during the recent trial of alendronate in male osteoporosis , from 197 of the original 241 participants , were assayed for testosterone , estradiol , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , bone specific alkaline phosphatase [ BSAP ( serum ) ] , and N telopeptide of type 1 collagen corrected for creatinine [ NTx ( urine ) ] . ^^^ Together with fracture and densitometry data from the original study , relationships were examined between BMD and serum IGF 1 , IGFBP 3 , testosterone , estradiol , BSAP , and urine NTx , both at baseline and during treatment with alendronate , to gain possible insights into the pathogenesis of male osteoporosis . ^^^ Statistically significant ( P < or= 0 . 05 ) associations were documented , at baseline , between the presence of vertebral fracture and each of serum IGF 1 , serum IGFBP 3 , serum free testosterone , total spine BMD , and total body BMD . ^^^ No statistically significant correlations were observed between any of the baseline variables ( IGF 1 , IGFBP 3 , estradiol , testosterone , and presence of vertebral fracture ) and the BMD response to alendronate at any site . ^^^ However , no statistically significant relationships were seen between any of the baseline variables ( age , body mass index , testosterone , estradiol , IGF 1 , IGFBP 3 , and prior fracture ) and change in BMD at any site . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
SGA children usually have growth factor levels within the normal range ; however , as a group , they display lower IGFBP 3 levels in relation to their IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thus , IGF 1 , IGFBP 3 and the phosphorylation status of IGFBP 1 in circulation are likely to be involved in growth regulation during the postnatal period in VLBW infants . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A recent nested case control study found that adults without IHD , but with low circulating IGF 1 levels and high IGF binding protein 3 levels , had a significantly increased risk of developing IHD during a 15 year follow up period . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The impact of progesterone on simultaneous , local secretion of IGFBP 3 and IGF 1 [ IGFBP 3 / IGF 1 index ] by human malignant and non malignant breast explants depends on tissue steroid receptor phenotype . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) limits the IGF 1 binding potential to its receptor . ^^^ AIM : To determine the ability of progesterone to stimulate simultaneous local IGF 1 and IGFBP 3 secretion by non malignant and malignant mammary tissue collected from different receptor phenotype tumours . ^^^ Later media were collected for IGF 1 and IGFBP 3 concentration analysis . ^^^ RESULTS : Progesterone increased ( p < 0 . 01 ) IGFBP 3 / IGF 1 index in ER ( ) PR ( ) non malignant tissue and decreased the IGFBP 3 / IGF 1 index in ER ( ) PR ( + ) , ER ( + ) PR ( ) non malignant explants . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relation of serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 to risk of prostate cancer ( United States ) . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) modulates the effects of IGF 1 , and independently induces apoptosis and inhibits cell growth . ^^^ Previous studies have inconsistently associated IGF 1 and IGFBP 3 with prostate cancer . ^^^ METHODS : Serum IGF 1 and IGFBP 3 were determined for 845 men ( 408 cases and 437 sibling controls ) . ^^^ RESULTS : Among all study subjects , only the molar ratio of IGF 1 to IGFBP 3 was associated with prostate cancer : comparing those in the highest to lowest quartiles gave an OR = 1 . 62 ( 95 % CI = 1 . 02 2 . 57 , trend p = 0 . 04 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH , GHBP , IGF 1 and IGFBP 3 were determined before , during and after GnRHa and DHT treatment . ^^^ Laboratory data at the prepubertal age showed markedly high GH , low GHBP , IGF 1 and IGFBP 3 levels . ^^^ Re evaluation at pubertal age showed normal basal serum IGFBP 3 levels and low but near normal IGF 1 levels . ^^^ We also noticed a sustained decrease in GH , IGF 1 and IGFBP 3 levels after blocking puberty , which was not affected by short and long term DHT treatment . ^^^ The decrease in IGF 1 and IGFBP 3 levels after GnRHa therapy , which was not reversed with DHT administration , suggests that sex steroids have , through oestradiol , a GH independent action on IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For postmenopausal subjects , the change in IGF 1 , IGF BP 1 and IGFBP 3 concentrations over the supplementation period did not differ between isoflavone or placebo phase . ^^^ RESULTS : : For premenopausal subjects , the change in IGF 1 , IGF BP 1 and IGF BP 3 assessed at different points of the menstrual cycle did not differ between isoflavone and placebo phase . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
He was short in stature , had high circulating levels of GH , and low circulating levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) . ^^^ His GH responses to the administration of growth hormone releasing hormone ( GHRH ) and L DOPA were normal , but his levels of IGF 1 and IGFBP 3 did not increase after the administration of exogenous GH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Eleven candidate genes were examined , including the androgen receptor , SRD5A2 , CYP 17 , CYP3A4 , vitamin D receptor , PSA , GST T 1 , GST M 1 , GST P 1 , IGF 1 , and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We determined the effect of raloxifene on IGF 1 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and leptin in premenopausal women at high risk for developing invasive breast cancer . ^^^ IGF 1 , IGFBP 3 , and leptin were measured by ELISA . ^^^ IGF 1 : IGFBP 3 molar ratio was stable ( mean change 0 . 014 ; P = 0 . 30 ; 95 % CI , 0 . 041 to 0 . 012 ) . ^^^ Increases in IGFBP 3 may potentially decrease the activity of circulating IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 , IGFBP 3 and c peptide and risk of hyperplasia and cancer of the breast in postmenopausal women . ^^^ Our objective was to assess the relationship between serum concentrations of insulin like growth factor 1 ( IGF 1 ) , its major binding protein ( IGFBP 3 ) , the ratio IGF 1 : IGFBP 3 , c peptide ( a marker of insulin secretion ) and the ratio c peptide : fructosamine ( a marker of insulin resistance ) and the risk of epithelial hyperplasia ( an established breast cancer risk factor ) and localized breast cancer among postmenopausal women . ^^^ Serum concentrations of IGF 1 , IGFBP 3 and the ratio IGF 1 : IGFBP 3 were not related to risk of either hyperplasia or breast cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a group of 87 postmenopausal women , we found that childhood exposure to the 1944 1945 Dutch famine was associated with increased plasma levels of IGF 1 and IGFBP 3 , whereas IGFBP 1 and 2 levels were weakly decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess the effect of pegvisomant induced serum insulin like growth factor 1 ( IGF 1 ) normalization on IGF binding proteins 1 , 2 , 3 ( IGFBP 1 , IGFBP 2 and IGFBP 3 ) , total , non bound ( 45 kDa ) and 150 kDa ternary complex associated IGFBP 3 , and in vivo IGFBP 3 proteolysis in patients with active acromegaly . ^^^ Serum IGF 1 normalization ( 699+ / 76 to 242+ / 28 microg / L , P < 0 . 0001 ) was associated with a fall in total IGFBP 3 ( 4345+ / 194 to 3283+ / 160 microg / L , P < 0 . 001 ) due to a reduction in 150 kDa ternary complex associated IGFBP 3 ( 3908+ / 160 to 3008+ / 140 microg / L , P < 0 . 0001 ) . 45 kDa IGFBP 3 and in vivo IGFBP 3 proteolysis were unaffected by GH receptor blockade ( 326+ / 13 to 330+ / 18 microg / L , P=0 . 86 ; 30+ / 3 . 5 to 30+ / 3 . 9 % , P=0 . 75 , respectively ) . ^^^ CONCLUSIONS : GH receptor blockade in patients with acromegaly lowers IGF 1 and 150 kDa IGFBP 3 ternary complex formation . 50 kDa ternary complex formation ( not in vivo IGFBP 3 proteolysis ) is GH dependent and measurement of 150 kDa ternary complex associated IGFBP 3 may provide useful information regarding treatment efficacy in patients with acromegaly . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We further report that serum IGF 1 , IGF 2 and IGFBP 3 all show positive correlations with myoblast retrieval in control patients , but that with the exception of IGFBP 3 these correlations are lost in malignancy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Assays of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were performed on cases and 2 controls matched on age , recruitment center and calendar time . ^^^ Controlling for smoking history and IGFBP 3 strengthened associations with cancer for both IGF 1 ( OR = 3 . 00 ; 95 % CI = 1 . 50 6 . 01 ; p ( trend ) 0 . 005 ) and IGF 2 ( OR = 2 . 02 ; 95 % CI = 1 . 07 3 . 84 ; p ( trend ) = 0 . 04 ) Associations between the IGFs and cancer risk were stronger for advanced cases . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Epidemiological evidence indicates that increased levels of IGF 1 , reduced levels of IGFBP 3 or an increased ratio of IGF 1 to IGFBP 3 in the circulation are associated with an increased risk for the development of several common cancers , including those of the breast , prostate , lung and colon . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It is thought that PSA cleaves insulin like growth factor binding protein 3 ( IGFBP 3 ) , providing increased local levels of IGF 1 , leading to tumor growth . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were also lower than control values . ^^^ Furthermore , the GH response to GHRH severely increased ; IGF 1 and IGFBP 3 levels did not significantly vary . ^^^ The insulin reduction may be mainly involved in the increase in the GH response to GHRH through various possible central and peripheral mechanisms while decreasing the peripheral sensitivity to GH itself , as shown by the stable nature of the IGF 1 and IGFBP 3 values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined associations of stature and body mass index with IGF 1 , IGF 2 , and IGF binding protein ( IGFBP ) 2 and IGFBP 3 in 274 men aged 50 70 yr to investigate whether variations in growth factor levels underlie associations of anthropometry with a number of adult diseases . ^^^ Height and leg and trunk length were not strongly associated with circulating levels of IGF 1 , IGF 2 , or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the study was 1 ) to evaluate the association of maternal serum levels of placental GH and IGF 1 with fetal growth , and 2 ) to establish reference data for placental GH , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) in normal pregnancies based on longitudinal measurements . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 levels were measured during standard ( GH 0 . 033 mg / kg / day 10 4 ) and low ( GH 0 . 011 mg / kg / day 10 4 ) dose IGFGTs at 0 , 12 , 36 and 84 h . ^^^ RESULTS : Basal IGF 1 and IGFBP 3 standard deviation scores ( SDS ) in ISS patients were 1 . 39 ( 2 . 4 1 . 16 ) and 0 . 45 ( 1 . 13 0 . 38 ) , respectively , IGF 1 being lower than IGFBP 3 ( P < 0 . 0001 ) . ^^^ In GH deficient patients , IGF 1 increased at each time point ( P < 0 . 05 ) and IGFBP 3 at 36 h ( P < 0 . 05 ) . ^^^ CONCLUSIONS : In children with ISS , basal IGF 1 and IGFBP 3 SDS values were below the mean , IGF 1 showing a greater response in both IGFGTs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the major carrier protein in serum for the IGF 1 , thus is an important functional modulator of it . ^^^ Controversial results have also been reported on the value of serum IGF 1 and / or IGFBP 3 in the detection of PC , especially of metastatic PC ; as increased , decreased or unchanged when compared to BPH . ^^^ The aim of the present study was to investigate whether serum IGF 1 and IGFBP 3 levels change in localized and metastasized PC cases compared with BPH as the control cases . ^^^ Serum IGF 1 and IGFBP 3 levels were measured by two site immunoradiometric assay kits , and serum total and free PSA levels were assayed by chemiluminescence method . ^^^ RESULTS : Serum IGF 1 levels in both localized and metastasized PC cases were similar to BPH cases ( 138 . 3 + / 58 . 2 , 137 . 7 + / 39 . 0 and 147 . 7 + / 44 . 2 ng / ml , respectively ) , whereas serum IGFBP 3 levels were lower in metastasized PC group than in BPH group ( 1 , 795 . 6 + / 305 . 6 and 2 , 196 . 0 + / 505 . 7 ng / ml ; p = 0 . 005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The data suggest that swine enteric disease is associated with reduced circulating IGF 1 and reductions in total IGFBP and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This effect might be related to the cessation of the inhibitory effect of proinflammatory cytokines on the synthesis of IGF 1 and IGF BP 3 in the liver . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Addition of IGFBP 3 inhibited IGF 1 mediated IGF type 1 receptor ( IGF IR ) phosphorylation and activation of the downstream molecule Akt in BALB / c 3T3 fibroblasts overexpressing human IGF IR ( 3T3 IGF IR ) and in two human colon cancer cell lines ( COLO 201 and HT 29 ) . ^^^ Coincubation of the IGF I / IGFBP 3 complex with MMP 7 restored IGF 1 mediated IGF IR phosphorylation and activation of Akt in these cell lines . ^^^ These results indicate that MMP 7 proteolysis of IGFBP 3 plays a crucial role in regulating IGF 1 bioavailability , thereby promoting cell survival . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neither cord serum IGF 1 nor IGFBP 3 explained the relationships that we previously reported between maternal influences and neonatal bone mass . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In colostrum fed calves , DEXA decreased ( P < 0 . 05 ) mRNA levels of IGF 1 ( esophagus , fundus , duodenum , and ileum ) , IGF 2 ( fundus ) , IGFBP 2 ( fundus ) , IGFBP 3 ( fundus ) , IGF1R ( esophagus , ileum , and colon ) , IGF2R ( fundus ) , GHR ( fundus ) , and InsR ( esophagus , fundus ) , but in formula fed calves DEXA increased mRNA levels of IGF 1 ( esophagus , rumen , jejunum , and colon ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , it has been suggested that GH , IGF 1 and IGFBP 3 may be major players in the hormonal or paracrine pathways that regulate bone cell metabolism . ^^^ The aim of this study was to clarify further this issue by investigating ( a ) the influence of serum levels of leptin , GH , IGF 1 and IGFBP 3 on bone mass in various skeletal sites and , ( b ) , the relationship between leptin and the GH / IGF axis . 363 healthy individuals were investigated . ^^^ BMD and serum leptin , GH , IGF 1 and IGFBP 3 serum levels were assessed . ^^^ No significant differences were found between men with normal and those with reduced BMD with regards to serum leptin , GH , IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dominant negative STAT5b completely inhibited GH stimulated transcription of genes encoding the growth promoting proteins IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) , which comprise the major circulating IGF 1 complex , and blocked expression of the GH inhibitors SOCS 1 , SOCS 2 , and CIS , but had little effect on induction of SOCS 3 . ^^^ Constitutive active STAT5b stimulated robust transcription of IGF 1 , ALS , and IGFBP 3 in the absence of hormone but did little to modify GH mediated activation of SOCS family genes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body mass index , circulating levels of sex steroid hormones , IGF 1 and IGF binding protein 3 : a cross sectional study in healthy women . ^^^ The objectives of the study were to examine the association of body mass index ( BMI ) with circulating androgens ( testosterone , androstenedione and dehydroepiandrosterone sulfate ( DHEAS ) ) , estrogens ( estrone and estradiol ) , sex hormone binding globulin ( SHBG ) , IGF 1 and IGF binding protein ( IGFBP ) 3 , and the relationship between sex steroids , IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At the same time , it was proposed that in 8 day old Sertoli cells the effects of FSH on phosphorylated PKB ( P PKB ) levels can be explained by a combination of increased secretion of endogenous IGF 1 , decreased IGF binding protein 3 ( IGFBP 3 ) production , and a synergistic action of FSH on IGF 1 dependent PI3K activation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The purpose of the current study was to investigate the association between insulin resistance ( which was measured using fasting blood C peptide ) and its joint association with insulin like growth factors ( IGF 1 , IGF 2 , and IGF binding protein 3 [ IGFBP 3 ] ) on the risk of breast carcinoma . ^^^ There was a statistically significant twofold to threefold increased risk of breast carcinoma for women in the highest quartile of C peptide , IGF 1 , or IGFBP 3 compared with women in the lowest quartiles . ^^^ Women with high levels of both C peptide and IGF 1 or IGFBP 3 also were found to have a substantially higher risk of breast carcinoma than those women with a high level of only one of these molecules . ^^^ The adjusted odds ratios ( ORs ) were 3 . 79 ( 95 % confidence interval [ 95 % CI ] , 2 . 03 7 . 08 ) for those with a higher level of both C peptide and IGF 1 and 4 . 03 ( 95 % CI , 2 . 06 7 . 86 ) for those with a higher level of both C peptide and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Basal and stimulated ( after administration of the hexapeptide Hexarelin ) GH levels were measured with IRMA ( Nichols ) ; IGF 1 and IGFBP 3 levels were measured with RIA ( Nichols ) . ^^^ However , desferrioxamine dose 10 years correlated with IGFBP 3 ( r = 0 . 56 , p < 0 . 05 ) and correlated inversely with IGF 1 binding ( r = 0 . 74 , p < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Thirty six prepubertal children infected by HIV , 12 boys and 24 girls , aged 4 12 years ( mean + / SD 7 + / 2 years ) , were tested for thyroid function ( serum T 4 , T 3 , TSH and free T 4 ) , morning serum cortisol level , serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) . ^^^ Two thirds and one third of the patients showed low IGF 1 and IGFBP 3 standard deviation scores ( SDS ) , respectively . ^^^ Statistical significance was found between CD4+ percentage and IGF 1 SDS , IGFBP 3 SDS , serum T 3 and free T 4 ( p value = 0 . 03 , 0 . 02 , 0 . 02 and 0 . 01 , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the complex , IGFBP 3 accounts for the majority of IGFBPs that interact with IGF 1 in circulation . ^^^ In a growth hormone time course experiment , both IGF 1 and IGFBP 3 message levels increased sharply , and after 24 hours of injection , IGF 1 and IGFBP 3 was increased 1 . 97 and 2 . 15 fold , respectively . ^^^ The relative message ratio of IGF 1 to IGFBP 3 was 1 . 58 , 1 . 09 , 0 . 94 , 0 . 91 , and 0 . 92 for 3 , 6 , 9 , 12 , and 24 hours , respectively . ^^^ This is the first report to demonstrate a fish counterpart of IGFBP 3 at the cDNA level and its message expression ratio with IGF 1 under growth hormone induction . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In training subjects GH treatment increased GH , IGF 1 , IGFBP 3 and ALS , and decreased IGBP 2 . ^^^ After cessation of treatment IGF 1 , IGFBP 3 and ALS approached basal values between 49 and 96 h . ^^^ In conclusion , increases in IGF 1 , IGFBP 3 , ALS , PIIIP and ICTP are all indicative of recent GH abuse or of acromegaly . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aims of the present study were ( 1 ) to investigate the influence of insulin like growth factor 1 ( IGF 1 ) on follicular size , on the secretion of oxytocin ( OT ) , progesterone ( P ) , estradiol ( E ) , IGF binding protein 3 ( IGFBP 3 ) , inhibin A , inhibin B and cAMP and on the expression of proliferation associated peptide PCNA , ERK related mitogen activated protein kinase ( MAPK / ERK1 , 2 ) and protein kinase A ( PKA ) in cultured porcine ovarian follicles ; ( 2 ) to examine the effects of OT on IGF 1 and on these functions ; and ( 3 ) to determine whether the effects of IGF 1 can be mediated by OT . ^^^ When given together with IGF 1 , the antiserum prevented the stimulatory action of IGF 1 on the proportion of enlarged follicles and on OT , IGFBP 3 and MAPK / ERK1 . ^^^ These observations demonstrate the involvement of IGF 1 and OT in the control of ovarian follicular size and follicular cell proliferation , progestagen , estrogen , IGFBP 3 , inhibin A and B secretion and in cAMP / PKA and MAPK / ERK1 dependent intracellular mechanisms . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The purpose of this work was to study the effect of insulin like growth factor 1 ( IGF 1 ) and its binding protein ( IGFBP 3 ) on the recovery of erectile function in a rat model for neurogenic impotence . ^^^ In all , 28 male Sprague Dawley rats were divided into four groups : seven underwent a sham operation ; seven underwent bilateral cavernous nerve freezing ( control group ) ; seven underwent bilateral cavernous nerve freezing followed by intraperitoneal injection of IGF 1 ; and seven underwent bilateral cavernous nerve freezing followed by intraperitoneal injection of IGFBP 3 . ^^^ In the sham and IGF 1 group , there were significantly higher maximal intracavernous pressures compared to the IGFBP 3 complex and the control group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Increased IGF 1 : IGFBP 3 ratio in patients with hepatocellular carcinoma . ^^^ AIM : The aim of this prospective study was to investigate the potential implication of IGF 1 and its major binding protein ( IGFBP 3 ) in the development of hepatocellular carcinoma . ^^^ PATIENTS AND METHODS : IGF 1 and IGFBP 3 were measured in 150 healthy subjects , 40 patients with liver cirrhosis and 63 with liver cirrhosis and untreated hepatocellular carcinoma . ^^^ The ratio between IGF 1 and IGFBP 3 was also calculated . ^^^ RESULTS : Serum IGF 1 ( 70 + / 10 and 65 + / 7 vs . 185 + / 6 . 4 microg / l , P < 0 . 001 ) and IGFBP 3 levels ( 1225 + / 113 and 984 + / 67 vs . 3017 + / 80 microg / l , P < 0 . 001 ) were lower in patients with liver cirrhosis , without or with hepatocellular carcinoma , than in controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 were measured before and every 6 months during therapy and bone age was evaluated yearly . ^^^ During treatment , IGF 1 levels ranged from < or = 3 to +2SD and IGFBP 3 levels ranged from 3 to +2SD , in both groups . ^^^ We observed no statistically significant differences between the two groups regarding chronological age , bone age , height at the beginning and at the end of therapy , pubertal development , duration of rhGH treatment and IGF 1 and IGFBP 3 levels ( P > 0 . 05 ) . ^^^ Neither the clinical nor the hormonal parameters , IGF 1 and IGFBP 3 levels , were useful to predict the development of these features . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF BP 3 , and GH serum levels after stimulation tests in prepubertal allergic boys . ^^^ The serum levels of insulin like growth factor 1 ( IGF 1 ) ( DSL 5600 Active TM IGF 1 Coated Tube IRMA [ DSL Lab Inc , USA ] ) and its binding protein ( IGF BP 3 ) ( DSL 6600 Active TM IGF BP 3 Coated Tube IRMA [ DSL Lab Inc , USA ] ) were within the normal range except for one A child . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High intakes of skimmed milk , but not meat , increase serum IGF 1 and IGFBP 3 in eight year old boys . ^^^ OBJECTIVE : To examine whether a high protein intake ( PI ) from either milk or meat , at a level often seen in late infancy , could increase s IGF 1 and s IGF I / s IGFBP 3 in healthy , prepubertal children . ^^^ At baseline and after 7 days , anthropometrical variables were measured , diet was registered ( 3 day weighed records ) , and s IGF 1 and s IGFBP 3 ( RIA ) were determined after fast . ^^^ The high milk intake increased s IGF 1 by 19 % ( P = 0 . 001 ) and s IGF I / s IGFBP 3 by 13 % ( P < 0 . 0001 ) . ^^^ CONCLUSIONS : High intake of milk and not meat , increased concentrations of s IGF 1 and s IGF I / s IGFBP 3 significantly . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) modulates the activity of IGF 1 , which exerts antiapoptotic action upon the myocardiocyte . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) modulates the activity of IGF 1 , which exerts antiapoptotic action upon the myocardiocyte . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Genotypes assessed were the 3 ' untranslated region poly ( A ) and the intron 8 BsmI polymorphisms of the VDR gene , a CA repeat polymorphism of the IGF 1 gene , the A / C polymorphism at nucleotide 202 of the IGFBP 3 , the Gly972Arg polymorphism of the IRS 1 gene , and the Gly1057Asp polymorphism of the IRS 2 gene . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A high circulating level of insulin like growth factor 1 ( IGF 1 ) and low IGF binding protein 3 ( IGFBP 3 ) level have been associated with increased breast density in premenopausal women . ^^^ Genetic polymorphisms in the IGF 1 and IGFBP 3 genes may influence breast and serum levels of these growth factors . ^^^ The aims of this study were to determine whether polymorphic variations in the IGF 1 and IGFBP 3 genes are associated with breast density , and serum IGF 1 and IGFBP 3 levels , and whether serum IGF 1 and IGFBP 3 levels are associated with mammographic density . ^^^ Blood samples were taken for DNA extraction to genotype the subjects for polymorphic variants in the two candidate genes , and for measurement of circulating levels of IGF 1 and IGFBP 3 . ^^^ However , there was no association between serum IGF 1 and IGFBP 3 levels and breast density among premenopausal women ( P > 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Autocrine effects of IGF 1 induced VEGF and IGFBP 3 secretion in retinal pigment epithelial cell line ARPE 19 . ^^^ In this study we examined IGF 1 induced HIF 1alpha expression , VEGF and IGFBP 3 secretion , and the autocrine actions of VEGF and IGFBP 3 on these processes in the spontaneously transformed RPE cell line ARPE 19 . ^^^ Cells were treated with CoCl ( 2 ) , IGF 1 , recombinant human ( rh ) IGFBP 3 , and rhVEGF . ^^^ Western and ligand blot analyses of RPE cell conditioned medium confirmed that IGF 1 stimulated VEGF and IGFBP 3 secretion . rhVEGF stimulated IGFBP 3 secretion in an IGF 1 and HIF 1alpha independent manner , whereas rhIGFBP 3 attenuated IGF 1 induced VEGF secretion . ^^^ These findings demonstrate the multifaceted autocrine regulation of IGF 1 induced VEGF secretion by IGFBP 3 secreted in response to both IGF 1 and , to a lesser extent , VEGF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GHRA was administered in a dose previously shown to block GH action without an effect on circulating GH or IGF 1 levels . hIGFBP 1 administration increased stimulated circulating GH levels and serum total IGF 1 and IGFBP 3 levels . ^^^ Coadministration of GHRA abolished the hIGFBP 1 induced increase in serum IGF 1 and IGFBP 3 levels , whereas stimulated GH levels remained increased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have shown that human intestinal smooth muscle cells produce IGF 1 and IGF binding protein 3 ( IGFBP 3 ) . ^^^ IGFBP 3 inhibits the binding of IGF 1 to its receptor and thereby inhibits IGF 1 stimulated growth . ^^^ In several carcinoma cell lines and some normal cells , IGFBP 3 regulates growth independently of IGF 1 . ^^^ The aim of the present study was to determine whether IGFBP 3 acts independently of IGF 1 and to characterize the mechanisms mediating this effect in human intestinal smooth muscle cells . ^^^ The direct effects of IGFBP 3 were determined in the presence of an IGF 1 receptor antagonist to eliminate its IGF 1 dependent effects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Here we investigate the role of the phosphatidylinositol 3 kinase ( PI3K ) and MAPK pathways in the regulation of IGFBP 3 expression by IGF 1 and TGFalpha in bovine MECs . ^^^ IGF 1 and TGFalpha also stimulated IGFBP 3 mRNA and protein levels . ^^^ Pretreatment with the PI3K inhibitor LY 294002 or knockdown of p 85 with small interfering RNA inhibited IGF 1 or TGFalpha stimulated IGFBP 3 expression . ^^^ In contrast to TGFalpha , IGF 1 retained the ability to partially increase IGFBP 3 mRNA levels in the presence of MAPK kinase 1 inhibitors , indicating that IGF 1 may activate alternative substrates of the PI3K pathway that are involved in IGFBP 3 regulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Moreover , IGFBP 3 increased IGF 1 mRNA and dramatically enhanced IGF 1 release . ^^^ IGF 1 receptor ( IGF IR ) and its downstream signaling pathways Akt and ERK were phosphorylated by IGFBP 3 , whereas inhibition of IGF IR phosphorylation with tyrphostin AG 1024 suppressed the antiapopoptic effect of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NO production and protein expression of NOS , IGF 1 receptor ( IGF 1R ) and IGF binding protein 3 ( IGFBP 3 ) were measured by Griess assay and Western blot analysis , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 induced VEGF and IGFBP 3 secretion correlates with increased HIF 1 alpha expression and activity in retinal pigment epithelial cell line D 407 . ^^^ Epifluorescence microscopy demonstrated a positive correlation between HIF 1 alpha expression and nuclear localization , VEGF and IGFBP 3 synthesis and export , and IGF 1 action . ^^^ Western and ligand blot analyses of RPE cell conditioned medium indicated that IGF 1 induced increases in VEGF and IGFBP 3 secretion . ^^^ Cells grown on Transwell inserts exhibited constitutive apical secretion of VEGF and IGFBP 3 , which increased on apical or basolateral treatment with IGF 1 . ^^^ CONCLUSIONS : IGF 1 stimulates increased HIF 1 alpha expression as well as VEGF and IGFBP 3 secretion in D 407 cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 effectively inhibited IGF 1 and 2 stimulation with detectable effects at concentrations equimolar to the growth factor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Among these targets , the insulin like growth factor binding protein 3 gene ( IGFBP 3 ) , a major regulator of insulin like growth factor 1 ( IGF 1 ) proliferation and survival signaling , was strongly induced upon treating Ewing cells with EWS / FLI 1 specific small interfering RNAs . ^^^ Finally , IGFBP 3 induced Ewing cell apoptosis relies on both IGF 1 dependent and independent pathways . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were measured in prediagnostic blood samples from a total of 281 men who were subsequently diagnosed with prostate cancer after recruitment ( median , 5 years after blood collection ) and from 560 matched controls . ^^^ RESULTS : Logistic regression analyses showed increases in prostate cancer risk with increasing plasma peptide levels , up to an odds ratio ( OR ) for top versus bottom quartile of IGF 1 of 1 . 67 ( 95 % CI , 1 . 02 to 2 . 71 ; Ptrend = . 05 ) , which was attenuated after adjustment for IGFBP 3 to an OR of 1 . 47 ( 95 % CI , 0 . 81 to 2 . 64 ; P ( trend ) = . 32 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
If IGF 1 and IGFBP 3 are found to correlate with a high incidence of testicular GCT , they might be useful surrogate markers for diagnosis and surveillance of tumour growth , and an early screening method to identify an increased risk of this type of cancer in the first degree young male relatives of these patients . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In subjects treated with GH first ( group A , n = 6 ) , GH treatment increased total IGF 1 ( P < 0 . 05 ) and IGF binding protein 3 ( P < 0 . 01 ) after 7 d , but these levels subsequently returned to pretreatment levels after 14 d . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Reevaluation of growth hormone deficiency during and after growth hormone ( GH ) treatment : diagnostic value of GH tests and IGF 1 and IGFBP 3 measurements . ^^^ The aim of this study was to reevaluate patients diagnosed as GHD at completion or reconfirm the diagnosis before completion of GH treatment by retesting with provocative tests , and to evaluate the value of IGF 1 and IGFBP 3 levels in the diagnosis of GHD . ^^^ Fifty ( 33 M , 17 F ) patients with GHD ( peak GH level < 0 . 46 pmol / l ( 10 ng / ml ] ) in two pharmacological tests were retested and IGF 1 and IGFBP 3 levels measured . ^^^ The sensitivities of IGF 1 and of IGFBP 3 were 70 % and 67 % , respectively , and the specificities were 100 % , when peak GH cutoff is taken as 0 . 46 pmol / l ( 10 ng / ml ) for the diagnosis of GHD . ^^^ The sensitivities of IGF 1 and IGFBP 3 increased to 76 . 5 % and 73 . 5 % when the cutoff level for GHD is taken as 0 . 32 pmol / l ( 7 ng / ml ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess and compare the effect of conjugated estrogen and of the selective estrogen receptor modulator raloxifene on serum levels of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) and on the IGF I / IGFBP 3 ratio . ^^^ MAIN OUTCOME MEASURE ( S ) : At baseline and after 6 , 12 , and 24 months of treatment , serum levels of IGF 1 , IGFBP 3 , and insulin were measured , and an IGF I / IFGBP 3 ratio was calculated . ^^^ CONCLUSION ( S ) : Long term use of both CEE and raloxifene decreases serum IGF 1 and the IGF I / IGFBP 3 ratio , but , unlike CEE , raloxifene produced a significant yet small increase in IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Assays of IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , and free and total PSA were performed . ^^^ When all biopsies were included , the performance of the free / total PSA index ( AUC 0 . 73 ) was significantly greater than for IGF 1 ( AUC 0 . 59 ; P < 0 . 0001 ) , IGF I / PSA ratio ( AUC 0 . 65 ; P = 0 . 002 ) , IGF 1 + IGFBP 3 ( AUC 0 . 59 ; P < 0 . 0001 ) , IGF 2 ( AUC 0 . 66 ; P = 0 . 002 ) , and IGF 2 + IGFBP 3 ( AUC 0 . 67 ; P = 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Before and after training , we measured serum levels of both total and free IGF 1 , IGFBP 1 and IGFBP 3 as well as plasma levels of branched chain amino acids ( valine , leucine and isoleucine ) and glucose from 26 cadets ( 21 + / 2 yr ) . ^^^ Total and free IGF 1 levels were decreased after training from 228 + / 12 to 160 + / 7 ng / ml and from 0 . 80 + / 0 . 08 to 0 . 52 + / 0 . 06 ng / ml , p < 0 . 001 respectively ) as well as IGFBP 3 ( p < 0 . 001 ) , while IGFBP 1 levels were increased ( p < 0 . 001 ) . ^^^ There were correlations between changes in total IGF 1 and IGFBP 3 ( p < 0 . 05 ) and between free IGF 1 and IGFBP 1 ( p < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIM OF THE STUDY : This study explored dietary determinants of circulating IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels among premenopausal women from different ethnic groups . ^^^ Serum concentrations of IGF 1 and IGFBP 3 were measured by double antibody ELISA . ^^^ After creating quartiles for 13 food and 13 nutrient density variables , least square means of IGF 1 , IGFBP 3 , and the IGF I / IGFBP 3 ratio were calculated by quartile , while adjusting for age , ethnicity , body mass index , and year of laboratory analysis . ^^^ RESULTS : Whereas body mass index and dietary intakes varied significantly by ethnicity , IGF 1 , IGFBP 3 , and their ratio were similar by group . ^^^ As the only food , fish showed a suggestive inverse association with IGF 1 and the IGF I / IGFBP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIM : To investigate the relationship between insulin like growth factor 1 , 2 ( IGF 1 and IGF 2 ) , IGF binding protein 3 ( IGFBP 3 ) and Child Pugh score in patients with liver cirrhosis , and to search for potential clinical markers of liver function . ^^^ Clinical significance of serum IGF 1 , IGF 2 and IGFBP 3 in liver cirrhosis . ^^^ Serum levels of IGF 1 , IGF 2 and IGFBP 3 were measured by immunoradiometric assay . ^^^ RESULTS : Serum IGF 1 , IGF 2 and IGFBP 3 levels were significantly lower in patients with cirrhosis than in controls , and serum concentrations of IGF 1 , IGF 2 and IGFBP 3 were associated with the severity of liver dysfunction , and dropped sharply during the progression of liver failure . ^^^ Concentrations of IGF 1 < 30 ng / mL , IGF 2 < 200 ng / mL and IGFBP 3 < 6 ng / mL implied a negative prognosis for patients with liver cirrhosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : This study aims to determine the relation between anabolic hormones , Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , growth parameters , and clinical status in prepubertal cystic fibrosis ( CF ) patients . ^^^ METHODS : Serum concentrations of IGF 1 and IGFBP 3 were measured in 37 CF and 23 healthy subjects , whose mean ages were 5 . 02 + / 3 . 06 and 5 . 27 + / 2 . 82 , respectively . ^^^ RESULTS : Serum IGFBP 3 of CF patients showed significantly lower concentrations than healthy subjects ( 2457 vs . 3249 ng / mL ) ( P < 0 . 05 ) , whereas IGF 1 levels did not ( 123 . 35 vs . 149 . 8 ng / mL ) . ^^^ There was significant positive correlation between IGF 1 and IGFBP 3 with HSD ( r = 0 . 62 ; r = 0 . 79 ) and BMISD ( r = 0 . 39 ; r = 0 . 50 ) . ^^^ CONCLUSION : Decreased serum IGF 1 and IGFBP 3 levels may reflect growth retardation in CF . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : In both trials , increases in serum sex hormone binding globulin were noted , as were decreases in insulin like growth factor ( IGF ) 1 and the IGF 1 : IGF binding protein 3 ratio ( P < 0 . 007 versus control / placebo ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To describe in a 5 year old Caucasian male with mitochondrial cytopathy , a biochemical growth hormone ( GH ) deficiency associated with normal GH biological activity as evaluated by Nb 2 cell bioassay and normal serum IGF 1 and IGFBP 3 values increasing slightly after GH administration . ^^^ Serum IGF 1 and IGFBP 3 concentrations were measured with RIA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PURPOSE : To examine whether serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) concentrations , determined early after the onset of stroke , are predictive of clinical outcome in elderly patients . ^^^ Fasting blood samples were collected within 24 hours of admission for IGF 1 and IGFBP 3 measurement . ^^^ Univariate and multiple logistic regression analyses , with adjustment for other related clinical covariates , were used to assess the relation of IGF 1 and IGFBP 3 to poor outcome , defined as severe disability ( Barthel index < 60 / 100 ) or death , at 1 month ( or at discharge ) , 3 months , and 6 months . ^^^ The mean IGF 1 / IGFBP 3 molar ratio was also lower in stroke patients ( 0 . 12 + / 0 . 07 vs . 0 . 19 + / 0 . 09 , P adjusted for age < 0 . 0001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dietary and lifestyle correlates of plasma insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) : the multiethnic cohort . ^^^ High circulating concentration of insulin like growth factor 1 ( IGF 1 ) and low circulating concentration of IGF binding protein 3 ( IGFBP 3 ) have been associated with increased risk for breast , prostate , and colorectal cancers . ^^^ Building on previous work in the Multiethnic Cohort ( MEC ) showing significant differences in IGF 1 levels across racial / ethnic groups , we investigated which lifestyle and dietary factors are associated with levels of IGF 1 and IGFBP 3 in a random sample of 1 , 000 MEC participants , which included Native Hawaiian , African American , Japanese , Latino , and White men and women . ^^^ In multivariate analyses , IGF 1 levels were lower and IGFBP 3 were higher in females versus males . ^^^ IGF 1 and IGFBP 3 declined with increasing age in both genders . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Synthesis and characterization of biotinylated forms of insulin like growth factor 1 : topographical evaluation of the IGF 1 / IGFBP 2 AND IGFBP 3 interface . ^^^ To identify key domains of IGF 1 involved in the interaction with IGFBP 2 and IGFBP 3 , we employed IGF 1 selectively biotinylated on residues Gly 1 , Lys 27 , Lys 65 , and Lys 68 . ^^^ All monobiotinylated species of IGF 1 exhibited high affinity ( approximately 0 . 1 0 . 2 nM ) for IGFBP 2 and IGFBP 3 in solid phase binding assays . ^^^ When taken together , these results suggest that , once bound to IGFBP 2 or IGFBP 3 , the biotin moieties of N ( alpha ) ( Gly1b ) IGF 1 and N ( epsilon ) ( Lys27b ) IGF 1 are inaccessible to NeutrAvidin peroxidase , the secondary binding component . ^^^ In BIAcore analysis , IGFBP 2 and IGFBP 3 bound only to the N ( epsilon ) ( Lys65 / 68b ) IGF 1 coated flowcell of a biosensor chip , confirming the inaccessibility of Gly 1 and Lys 27 when IGF 1 is bound to IGFBP 2 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The hIGF 2 transgene affects neither endogenous IGF 1 and IGF 2 expression , nor the expression of IGFBP 3 , which is located in the choroid plexus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The subjects ' serum samples were analyzed for prostate specific antigen ( PSA ) and the following hormones , which may be related to prostate cancer : testosterone , sex hormone binding globulin , 3alpha androstanediol glucuronide , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) . ^^^ The mean age adjusted hormone levels , as well as testosterone / sex hormone binding globulin and IGF 1 / IGFBP 3 ratios , also were not significantly different statistically across the subgroups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To investigate this observation further , we measured serum IGF 1 , IGF 2 and IGF binding protein 3 concentrations in 232 men and 210 women ( aged 55 64 years ) , and related peptide levels to smoking characteristics . ^^^ For male former vs never smokers , there were no differences in mean IGF 1 and IGFBP 3 concentrations , suggesting that smoking cessation is associated with normalisation of peptide concentrations . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Birthweight , cord blood insulin , proinsulin , 32 33 split proinsulin , leptin , IGF 1 , IGFBP 3 , cortisol . ^^^ Individually , insulin , insulin propeptides , leptin , IGF 1 and IGFBP 3 were significantly ( P < 0 . 05 ) correlated with birthweight ( in ODM and Control ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We determined serum levels of insulin like growth factors ( IGF 1 , IGF 2 ) and binding proteins ( IGFBP 2 and IGFBP 3 ) at diagnosis ( 1 ) , during ( 2 ) and after therapy ( 3 ) in 28 children treated for ALL . ^^^ RESULTS : 1 ) Serum absolute levels of IGF 1 , IGF 2 , IGFBP 3 rose and IGFBP 2 decreased during analysis . 2 ) Mean values of IGF 1 SDS were similar at diagnosis , during and after treatment ( 1 . 78+ / 0 . 9 vs . 1 . 86+ / 1 . 36 vs . 1 . 8+ / 0 . 83 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The purpose of the study was the evaluation of growth hormone ( GH ) , insulin like growth factor ( IGF 1 ) , insulin like growth factor binding protein ( IGFBP 3 ) and vascular endothelial growth factor ( VEGF ) in adolescents with type 1 diabetes as well as the relationship between the concentration of examined proteins and the early development of diabetic microangiopathy . ^^^ GH , IGF 1 , IGFBP 3 and VEGF were determined with use of ready kits . ^^^ The levels of IGF 1 were lower in adolescents with type 1 diabetes than in controls ( 283 . 48+ / 117 . 36 vs . 427 . 95+ / 177 . 48 ng / ml , p < 0 . 05 ) , as well as the levels of IGFBP 3 ( 5555 . 21+ / 1158 vs . 6622 . 18+ / 1110 pg / ml , p < 0 . 05 ) . ^^^ CONCLUSIONS : The impaired activity of GH IGF 1 IGFBP 3 axis may be responsible for the development of diabetic microangiopathy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of the present study was to examine the levels of insulin like growth factor ( IGF 1 ) and binding protein 3 ( IGFBP 3 ) in acute coronary syndrome ( ACS ) and their relationship with prognosis . ^^^ Serum IGF 1 and IGFBP 3 levels were determined by radioimmunoassay method . ^^^ There was no relationship between IGF 1 , IGFBP 3 levels and cardiovascular events occurred during 90 days of follow up . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating free insulin like growth factor ( IGF ) 1 , total IGF 1 , and IGF binding protein 3 levels do not predict the future risk to develop prostate cancer : results of a case control study involving 201 patients within a population based screening with a 4 year interval . ^^^ Recent studies have reported that serum IGF 1 levels in the highest quartile of the normal range and IGF binding protein 3 ( IGFBP 3 ) in the lowest quartile of the normal range are associated with an increased risk of future prostate cancer and / or presence of prostate cancer . ^^^ To determine whether circulating free IGF 1 , total IGF 1 , and IGFBP 3 levels can predict future prostate cancer risk , we prospectively studied prostate cancer characteristics in a cohort of men during two rounds ( mean interval , 4 yr ) of a population based screening study for prostate cancer . ^^^ At baseline , total IGF 1 , free IGF 1 , and IGFBP 3 levels and prostate volume of cases with prostate cancer were not different from those of healthy controls . ^^^ Stepwise forward logistic regression analysis showed that only PSA levels at baseline and PSA at round 2 after 4 yr are good predictors of prostate cancer , whereas total IGF 1 , free IGF 1 , and IGFBP 3 did not predict the development of prostate cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the effects of recombinant human ( rh ) IGF 1 [ complexed in equimolar ratio with rhIGF binding protein ( BP ) 3 ( rhIGF I / IGFBP 3 ) ] replacement on overnight GH levels and insulin sensitivity in type 1 diabetes . ^^^ We found dose dependent increases in circulating IGF 1 and IGFBP 3 concentrations after rhIGF I / IGFBP 3 . ^^^ Thus , restoration of circulating IGF 1 and IGFBP 3 levels with rhIGF I / IGFBP 3 suppresses GH secretion in adolescents with type 1 diabetes , leading to reduced insulin requirements and improvements in insulin sensitivity . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^ A trend of higher IGFBP 3 secretion after IGF 1 stimulation ( P=0 . 09 ) and 3 . 5 5 times higher mRNA expression of IGFBP 3 in basal conditions was found in NSD 1 ( + / ) fibroblasts in comparison to controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and lipid levels were determined centrally . ^^^ Serum IGF 1 and IGFBP 3 were significantly lower at baseline in CO compared with AO patients , similar to Caucasian patients , and were increased in both onsets following GH treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Maternal questionnaires were completed , and laboratory measurements of several variables , including IGF 1 , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) , were made for a total of 331 apparently healthy full term newborns , from whom routine blood samples were taken during the first 5 days of their life . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Here we find that insulin and ( Q3A4Y15L16 ) IGF 1 ( an IGF 1 analog that has a low affinity for IGFBP 3 ) antagonize growth inhibition by IGFBP 3 in mink lung epithelial cells ( Mv1Lu cells ) stimulated by serum . ^^^ The IGFBP 3 induced dephosphorylation of IRS 2 is prevented by cotreatment of cells with insulin , ( Q3A4Y15L16 ) IGF 1 , or TbetaR V / LRP 1 antagonists . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Clinical studies have established elevated serum insulin like growth factor ( IGF 1 ) content and IGF 1 : IGF binding protein 3 ( IGFBP 3 ) ratio as risk factors for these same malignancies . ^^^ Overexpression of COX 2 in NSCLC cells enhanced the antiapoptotic and mitogenic effects of IGF 1 and IGF 2 , facilitated the autophosphorylation of the type 1 IGF receptor , increased class IA phosphatidylinositol 3 ' kinase activity , and decreased expression of IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Patients with ectopic hyperintense signal were shorter in height , had lower IGF 1 and IGFBP 3 levels , and reduced GH response after clonidine . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
NBI 31772 inhibited the binding of 125I labeled IGF 1 to IGFBP 3 at nanomolar concentrations . ^^^ It antagonized the inhibitory effect of IGFBP 3 on IGF 1 dependent proteoglycan synthesis by rabbit chondrocytes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body weights of the double mutants were reduced markedly during the first 3 4 months of age , and adults had striking reductions in femur length , plasma IGF 1 and IGF binding protein 3 concentrations , and femoral bone mineral density . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No independent associations of dietary factors with IGF 1 or IGFBP 3 concentrations were observed . ^^^ However , in premenopausal women alcohol intake was inversely associated with IGF 1 and positively associated with IGFBP 3 after mutual adjustment . ^^^ CONCLUSIONS : In this study population , with limited variation in dietary intake , total energy , protein , phytoestrogens and lycopene were not associated with IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : The odds ratios for endometrial cancer comparing the highest versus the lowest tertile of various IGF components were as follows : 0 . 5 [ 95 % confidence interval ( CI ) 0 . 2 1 . 2 ] for free IGF 1 , 1 . 1 ( 95 % CI 0 . 5 2 . 6 ) for total IGF 1 , 1 . 2 ( 95 % CI 0 . 6 2 . 6 ) for total IGF 2 , 2 . 4 ( 95 % CI 1 . 0 5 . 9 ) for IGF binding protein ( IGFBP ) 1 and 0 . 8 ( 95 % CI 0 . 4 2 . 0 ) for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGF binding protein 3 were significantly higher after 6 months of GH treatment and 6 months after discontinuation of GH treatment than at baseline . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Using partial correlation analysis and longitudinal analysis of data collected at the year 2 , year 7 , and year 10 examinations , the authors assessed the associations of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) with cardiovascular disease risk factors in 544 Black and 747 White male participants in the Coronary Artery Risk Development in Young Adults ( CARDIA ) Male Hormone Study who were aged 20 34 years at year 2 ( 1987 1988 ) . ^^^ Cross sectionally , there were some inverse associations between IGF 1 and lipid levels in White men ( strongest r = 0 . 095 ( p = 0 . 02 ) for total cholesterol at year 7 ) and positive associations between IGFBP 3 and lipid levels in Black and White men ( for log ( triglycerides ) , r = 0 . 072 0 . 136 ) . ^^^ These findings do not support a strong link between IGF 1 and IGFBP 3 and blood pressure , but they do support the possibility of important relations between IGFBP 3 and lipid levels in young adult men . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relationship between growth hormone IGF 1 IGFBP 3 axis and serum leptin levels with bone mass and body composition in patients with rheumatoid arthritis . ^^^ Growth hormone was higher in RA patients ( P=0 . 0001 ) while IGF 1 and IGFBP 3 did not differ between the three groups . ^^^ In this study , we aimed to evaluate the growth hormone ( GH ) insulin like growth factor 1 ( IGF 1 ) insulin like growth factor binding protein 3 ( IGFPB 3 ) axis and serum leptin levels in patients with RA and to determine whether these hormonal / growth factors may influence bone mass and body composition in RA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All samples were analyzed for total and free IGF 1 and IGF 2 , IGFBP 3 and IGFBP 3 proteolysis , total and non phosphorylated ( np ) IGFBP 1 , and IGFBP 1 complexed IGF 1 . ^^^ CONCLUSION : We found evidence of increased free IGF 1 and increased IGF 1 in binary complexes during pregnancy , possibly caused by IGFBP 3 proteolysis and decreased ternary complex formation . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To study the potential endocrine basis for this reciprocal relationship we examined the in vivo effects of the androgens , testosterone ( T ) and 11 ketotestosterone ( 11 KT ) , on plasma growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) and 41 kDa IGF binding protein ( 41 kDa IGFBP ) ( putative IGFBP 3 ) in coho salmon , Oncorhynchus kisutch . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Levels of GH , IGF 1 , and IGFBP 3 were above the 95th percentile in the serum of the 3 year old girl with a mono allelic deletion of the IGF1R gene , suggesting IGF 1 resistance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition was assessed by dual energy 10 ray absorptiometry and bioelectrical impedance spectrometry ; functional capacity by hand grip strength , quadriceps strength , and 30 second sit to stand test ; systemic inflammation by tumor necrosis factor alpha ( TNF alpha ) and TNF receptor 1 ( TNFR 1 ) ; serum and muscle IGF 1 and IGFBP 3 by radioimmunoassay ; and fragmentation of serum IGFBP 3 by Western immunoblotting . ^^^ Although serum IGF 1 and IGFBP 3 levels did not differ between the groups ( P = . 295 and . 379 respectively ) , fragmented IGFBP 3 levels were increased ( 53 . 1 + / 16 . 0 versus 29 . 81 + / 15 . 3 % , P < . 005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Exogenous IGFBP 3 strongly inhibited IGF 1 driven proliferation in SK RC 52 , but worked in synergy with IGF 1 in Caki 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the relationship between levels of insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) in antenatal maternal serum and in fetal cord blood at delivery . ^^^ RESULTS : There was no significant correlation between maternal IGF 1 , IGF 2 , or IGFBP 3 levels and fetal acidosis . ^^^ Maternal and fetal insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) and IGF BP 3 , and their relationship to fetal acidosis at delivery . ^^^ Maternal IGF 1 , IGF 2 and IGF BP 3 were assessed on maternal blood at booking and in fetal blood by cord blood analysis at delivery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The present study was carried out to compare serum levels of leptin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , homeostasis model assessment ( pancreatic beta cell function ) ( HOMA ( % B ) ) and homeostasis model assessment ( tissue insulin sensitivity ) ( HOMA ( % S ) ) in women with mild and severe pre eclampsia and normotensive pregnant women ; and to evaluate the possible relationships between these parameters in the pathogenesis of pre eclampsia . ^^^ Serum levels of IGF 1 and IGFBP 3 were measured with a two site immunoradiometric assay . ^^^ Serum levels of IGF 1 and IGFBP 3 were significantly lower in women with SPE and MPE compared with NPW ( p < 0 . 001 ) . ^^^ We conclude that pre eclampsia is associated with insulin resistance ; and that existing hyperinsulinemia and insulin resistance in women with pre eclampsia seem not to correlate with leptin and birth weight , but may correlate positively with IGF 1 and IGFBP 3 . ^^^ Therefore we think that hyperleptinemia , low IGF 1 or IGFBP 3 , and insulin resistance may contribute to the pathogenesis of pre eclampsia . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effects of early nutrition intervention on IGF 1 , IGFBP 3 , intestinal development , and catch up growth of intrauterine growth retardation rats . ^^^ OBJECTIVE : To investigate the effects of early nutritional intervention on the serum insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , intestinal development , and catch up growth of intrauterine growth retardation ( IUGR ) rats by giving the IUGR new born rats different protein level diet . ^^^ Detected the serum IGF 1 , IGFBP 3 , body weight , body length , intestinal weight length , intestinal villi height ( VH ) , crypt depth ( CD ) , villi absorbing area ( VSA ) , mucous thickness ( MT ) , and disaccharidase at the 4th week . ^^^ RESULTS : ( 1 ) The SH group showed the fastest catch up growth , serum IGF 1 , IGFBP 3 , VH , and VSA were significantly higher than those of normal control group and IUGR control group . ^^^ The intestinal weight and length , and the activities of lactase and saccharase of the SH group also reached the normal control group level . ( 2 ) The SL group kept on small size , the serum IGF 1 , IGFBP 3 , and most of intestinal histological indexes were all significantly lower than other groups . ( 3 ) IGF 1 , IGFBP 3 were positively correlated to intestinal VH , VSA , saccharase , body weight and length . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , bone turnover markers ( Ost , ICTP ) , and anti growth hormone antibody ( GHAb ) were detected before and after r hGH treatment . ^^^ GHD Children had obviously reduced serum levels of IGF 1 , IGFBP 3 , and bone turnover markers ( Ost , ICTP ) compared with normal controls ( P < 0 . 01 ) , and these biochemical parameters improved significantly after treatment ( P < 0 . 01 ) . ^^^ CONCLUSIONS : ( 1 ) The growth stimulating effect and safety were confirmed in using China made r hGH in the treatment of GHD children for 6 months . ( 2 ) The measurements of serum IGF 1 and IGFBP 3 may serve as useful parameters in the diagnosis of GHD . ( 3 ) Serum Ost and ICTP are useful laboratory criteria for evaluating the effect of r hGH therapy in the early stage . ( 4 ) It is necessary to monitor serum levels of GHAb during r hGH therapy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The effect of the drug was assessed on plasma levels of gonadotropins luteinising hormone ( LH ) , follicle stimulating hormone ( FSH ) , of insulin , insulin like growth factor 1 ( IGF 1 ) , androstenedione , testosterone , dehydroepiandrosterone sulphate ( DHEAS ) , sex hormone binding globulin ( SHBG ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ Octreotide significantly reduced LH ( 31 . 8 % ) , insulin ( 52 % ) , IGF 1 ( 14 % ) , androstenedione ( 22 . 6 % ) and testosterone ( 20 % ) and increased IGFBP 3 ( 25 % ) . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of this work was to investigate the roles of IGF 1 and IGFBP 3 in esophageal cancer . ^^^ MATERIALS AND METHODS : We examined the circulating IGF 1 and IGFBP 3 concentration in 18 healthy controls and 66 esophageal cancer patients by ELISA and a ligand capture immunoassay . ^^^ RESULTS : The serum IGF 1 and IGFBP 3 levels were significantly elevated in patients compared with healthy subjects and there was a positive correlation between IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Spontaneous growth hormone secretion and IGF 1 : IGFBP 3 molar ratios in children born small for gestational age ( SGA ) . ^^^ OBJECTIVE : To analyze spontaneous nocturnal GH profiles , IGF 1 and IGFBP 3 serum levels , as well as IGF 1 : IGFBP 3 molar ratios in SGA children without postnatal catch up growth . ^^^ Overall , median IGF 1 levels were approximately one SDS below zero SDS ( p < 0 . 001 ) , whereas IGFBP 3 levels were normal in both groups . ^^^ Thus , the IGF 1 : IGFBP 3 molar ratios were significantly lower from zero ( p < 0 . 01 ) in SGA as well as in AGA children . ^^^ However , IGF 1 and IGFBP 3 SDS levels related either to chronological or to bone age did not differ significantly between SGA and AGA children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : We analysed the strength and independence of associations of angiographically assessed presence of CHD with BMI , systolic and diastolic blood pressure , total , high density lipoprotein ( HDL ) and LDL cholesterol , triglycerides , lipoprotein ( a ) , apolipoproteins A 1 and B , total and free IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 5 , acid labile subunit ( ALS ) , insulin , C peptide , testosterone , DHEAS and sex hormone binding globulin . ^^^ RESULTS : Using multivariate statistical analysis , the presence of CHD had significant positive associations with total IGF 1 , IGFBP 5 , ALS and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At baseline serum samples were assayed for GHBP ; serum IGF 1 and IGFBP 3 levels were measured both at baseline and 24 h after GH administration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Marked treatment effects were also observed on other growth parameters , serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
All four hormones increased gene and / or protein expression of PSA , IGF IR , IGF 1 , and IGFBP 2 and decreased that of AR , ERbeta , IGF 2 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Prospective studies have shown an association between elevated plasma levels of insulin like growth factor 1 ( IGF 1 ) and / or decreased levels of its major circulating carrier protein insulin like growth factor binding protein 3 ( IGFBP 3 ) and increased risk of major cancers . ^^^ We evaluated the association between constitutional , hormonal and clinical factors and IGF 1 and IGFBP 3 in 740 women , including 376 unaffected women and 364 women with intraepithelial neoplasia ( IEN ) or early invasive breast cancer enrolled in breast cancer chemoprevention trials , conducted at a single institution . ^^^ Women with early breast cancer had 21 % higher IGF 1 levels ( p = 0 . 033 ) and 19 % higher IGF I / IGFBP 3 molar ratio ( p = 0 . 047 ) than unaffected women . ^^^ In unaffected women , age was negatively associated with IGF 1 ( p = 0 . 002 ) and IGF I / IGFBP 3 ( p = 0 . 001 ) , while age at menarche was negatively associated with IGFBP 3 levels ( p = 0 . 043 ) . ^^^ In women with IEN or early breast cancer , IGF 1 levels were negatively associated with age ( p < 0 . 001 ) , and positively associated with prior biopsies for benign disease ( p = 0 . 013 ) , while age , parity and menopausal status were significant predictors of IGF I / IGFBP 3 molar ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : There have been several epidemiologic studies investigating the association between circulating levels of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and insulin in relation to the risk of prostate carcinoma , with conflicting results . ^^^ METHODS : In men who were diagnosed with prostate carcinoma ( cases ) between 1990 and 1999 ( n=174 ) , the levels of IGF 1 , IGFBP 3 , and insulin were measured on blood samples that were obtained 1 9 years prior to diagnosis ( mean , 3 . 4 years ) . ^^^ CONCLUSIONS : The IGF 1 level was not associated positively with the risk of prostate carcinoma ; however , an increase in the IGFBP 3 level was associated with a modest decrease in risk . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied by real time reverse transcription PCR , whether there are differences in the abundance of mRNA coding for IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGF 1R , IGF 2R , GHR , and InsR in compartmentalized layers ( fractions ) of jejunum and ileum of 5 d old calves fed colostrum . ^^^ In ileum , mRNA levels , expressed relative to housekeeping genes , differed ( P < 0 . 05 ) between fractions for IGF 1 ( LP > villi , crypts ) , IGF 2 , and IGFBP 3 ( villi > crypts , LP ) , GHR and InsR ( crypts > LP ) , IGFBP 2 ( crypts > villi , LP ) , and total RNA levels were greater ( P < 0 . 05 ) in LP and crypt than in villus fractions . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the serum and milk in active smoking and nonsmoking mothers , and their infants ' insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels . ^^^ RESULTS : There was no difference noted in either IGF 1 , IGFBP 3 or IGF I / IGFBP 3 ratios in serum and milk of mothers , and their infants ' serum samples according to maternal smoking . ^^^ CONCLUSION : This study demonstrated that maternal smoking ( 5 10 cigarettes / day ) did not influence the maternal and infant serum levels of IGF 1 and IGFBP 3 as well as the breast milk levels of these peptides . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 and 2 were measured by radioimmunoassay , IGFBP patterns were characterised by ligand affinity and immunoblotting , and a lectin binding assay was used to investigate the glyco component of IGFBP 3 complexes . ^^^ IGFBP profiles correlated with the amount of IGF present : patients with lower IGF 1 concentrations expressed decreased IGFBP 3 and elevated IGFBP 2 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fibroblast conditioned medium , as well as exogenous IGF 1 , increased protein synthesis in cardiac myocytes , and this increase was inhibited by antibodies against IGF 1 and IGF 1 receptor , IGF binding protein 3 , and IGF 1 receptor antagonist . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , acid labile subunit , and the bone and soft tissue markers , osteocalcin , carboxyl terminal propeptide of type 1 procollagen , carboxyl terminal cross linked telopeptide of type 1 collagen , and procollagen type 3 were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the higher expressing 5012 Tg strain , IGF 1 and murine IGFBP 3 levels , marker of GH action were increased . ^^^ Furthermore , there was a positive correlation between mutant IGFBP 3 levels and IGF 1 levels and between mutant IGFBP 3 levels and murine IGFBP 3 ( P = 0 . 002 and P < 0 . 001 , respectively ) . ^^^ The higher levels of IGF 1 and murine IGFBP 3 in the 5012 Tg strain suggest that the growth inhibitory effect of mutant IGFBP 3 may be compensated for by other mechanisms . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Stimulation of myofibroblastic Mller cells by IGF 1 and 2 , but not PDGF , further increased message abundance and production of IGFBP 2 , 4 , 5 , and 6 , but not IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : After adjustment for potential confounders , the multivariate odds ratios for the highest versus the lowest tertile of various IGF components were 0 . 6 ( 95 % confidence interval , CI : 0 . 2 1 . 4 ) for free IGF 1 , 0 . 4 ( CI : 0 . 1 1 . 5 ) for total IGF 1 , 2 . 6 ( CI : 0 . 9 6 . 9 ) for IGF binding protein ( IGFBP ) 1 , and 0 . 2 ( CI : 0 . 0 0 . 6 ) for IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total and free IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 protease were similar between groups ( all P > . 5 ) , whereas , in LIPO , IGFBP 1 and IGFBP 2 were reduced ( 36 % , P < . 05 and 50 % , P < . 01 ) . ^^^ In pooled groups , total IGF 1 , free IGF 1 , total IGF 2 , and IGFBP 3 , respectively , correlated inversely with age ( all P < . 01 ) . ^^^ IGFBP 3 protease correlated with free IGF 1 in pooled groups ( r ( p ) = 0 . 47 , P < . 02 ) , and in LIPO ( r ( p ) = 0 . 71 , P < . 007 ) controlling for age , total IGF 1 , and IGFBP 3 . ^^^ Furthermore , our data suggest that IGFBP 3 protease is a significant regulator of bioactive IGF 1 in HIV lipodystrophy . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , an important role of GH and IGF 1 in the phenotype of these animals was demonstrated by an overexpression of IGF binding protein 3 mRNA in the liver and increased levels of circulating IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In theca cells , IGF 2 decreased ( P < 0 . 05 ) IGFBP 2 mRNA levels , but had no effect on IGFBP 3 or 4 mRNA expression ( Exp . 3 ) ; IGF 1 did not affect ( P > 0 . 10 ) thecal IGFBP 2 , 3 or 4 mRNA levels . ^^^ Ligand blotting revealed that both IGF 1 and 2 increased IGFBP 2 and 5 ( protein ) and had no effect on IGFBP 3 ( protein ) , whereas IGF 1 ( but not IGF 2 ) increased IGFBP 4 ( protein ) , suggesting IGFBP 2 , 4 , and 5 are post transcriptionally regulated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For premenopausal breast cancer , we found nearly significant interactions between soy protein intake and IGF 1 levels ( P = 0 . 080 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels ( P = 0 . 057 ) . ^^^ The direction of the interaction appeared to be negative for IGF 1 levels but was positive for IGFBP 3 levels . ^^^ No interaction was evident between soy protein intake and IGF 1 or IGFBP 3 levels among postmenopausal women . ^^^ Our results suggest that soy protein intake may negatively modulate the effect of IGF 1 and may positively modulate the effect of IGFBP 3 levels on premenopausal breast cancer risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Our data demonstrated an increase in the levels of IGF 1 , phosphatidylinositol 3 ' kinase , phosphorylated Akt ( Thr 308 ) , and extracellular signal regulated kinase 1 / 2 with concomitant decrease in IGFBP 3 in dorso lateral prostate of TRAMP mice during the course of cancer progression , i . e . , as a function of age . ^^^ Continuous green tea polyphenol infusion for 24 weeks to these mice resulted in substantial reduction in the levels of IGF 1 and significant increase in the levels of IGFBP 3 in the dorso lateral prostate . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Serum free and total IGF 1 , IGFBP 3 , serum osteocalcin level , urinary deoxypyridinoline concentration , spontaneous interleukin 6 ( IL 6 ) release and broadband ultrasound attenuation ( BUA ) were evaluated in 44 prepubertal children who had perinatal HIV 1 infection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Free IGF 1 , total IGF 2 , IGFBP 3 , and GH binding protein were lower , whereas IGFBP 1 , IGFBP 1 bound IGF 1 , and IGFBP 2 were elevated compared with control values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We analyzed plasma ghrelin levels and their correlation with plasma levels of leptin , insulin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 1 ( IGFBP 1 ) , insulin like growth factor binding protein 2 ( IGFBP 2 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and integrated concentration of growth hormone ( ICGH ) before and after dietary intervention . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 as diagnostic markers of growth hormone deficiency in infancy . ^^^ METHODS : Serum insulin like growth factor 1 ( IGF 1 ) and serum IGF binding protein 3 ( IGFBP 3 ) levels were evaluated as diagnostic markers of GHD . ^^^ Measurements of IGF 1 and IGFBP 3 during the 1st year of life were analyzed in 11 patients clinically suspected of having GHD ( neonatal hypoglycemia , micropenis , or evidence of other pituitary hormone deficiencies ) , in whom the diagnosis was later verified . ^^^ RESULTS : The sensitivity of IGF 1 as a diagnostic marker of GHD was 90 % ( 10 out of 11 patients ) with a cutoff value of 2 standard deviations ( SD ) , and the sensitivity of IGFBP 3 measurements was 81 % ( 9 out of 11 patients ) with a cutoff value below 2 SD . ^^^ One patient had serial measurements before initiation of GH treatment where the IGF 1 was fluctuating ( 3 of 6 slightly above 2 SD ) , whereas all IGFBP 3 measurements were below 2 SD . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In all subjects we assessed plasma levels of ghrelin , leptin , soluble leptin receptor , insulin , IGF 1 , IGFBP 1 , IGFBP 3 and IGFBP 6 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were collected from the newborns no later than the fifth day of life for measurements of adiponectin and major IGF system components ( IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated the effect of TPO treatment ( 0 , 1 , 10 or 100 ng / ml ) on the following characteristics of cultured porcine ovarian follicles , determined using SDS PAGE and Western blotting , immunocytochemistry , RIA and ELISA : the expression of intracellular peptides associated with proliferation ( PCNA ) , apoptosis ( Bax ) , tyrosine kinase ( TK , phosphotyrosine ) , Cdc2 / p34 kinase , PKA and the transcription factor CREB 1 , and the secretion of progesterone , androstenedione , estradiol 17beta , oxytocin , inhibin A , inhibin B , IGF 1 , transforming growth factor 2beta ( TGF 2beta ) and IGF binding protein 3 ( IGFBP 3 ) . ^^^ Furthermore , TPO was able to inhibit androstenedione , estradiol , TGF 2beta and IGFBP 3 secretion , and to stimulate oxytocin , inhibin A , inhibin B and IGF 1 secretion . ^^^ The PKA blocker KT 5720 , when given alone , reduced the expression of Bax and TGF 2beta , augmented the expression of PKA , CREB and oxytocin , but did not influence the secretion of progesterone , androstenedione , estradiol , IGFBP 3 , inhibins A and B or IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To facilitate the diagnosis of GH deficiency and monitor GH therapy , we constructed two reference models to allow comparison of serum IGF binding protein ( IGFBP ) 3 concentrations and IGF 1 to IGFBP 3 ratios among children throughout childhood and adolescence . ^^^ This report presents equations for determining the sd score of IGFBP 3 and IGF 1 to IGFBP 3 measurements for individual patients . ^^^ The values of IGFBP 3 and IGF 1 to IGFBP 3 ratios were log transformed , and multiple linear regression analysis was used to identify models for converting serum concentrations into sd scores . ^^^ The best linear models explain 42 % of the variation in serum IGFBP 3 concentrations and 50 % of the variation in serum IGF 1 to IGFBP 3 concentrations . ^^^ The relationship between age and log ( IGF 1 to IGFBP 3 ratio ) was positive for boys in pre , early , and early midpuberty ( volume = 9 14 ml ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although several studies have assessed cross sectional correlates of serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , there are no longitudinal studies of the correlates of long term changes in these measures . ^^^ We examined the 8 year longitudinal associations of age , body mass index ( BMI ) , waist circumference , physical activity , number of cigarettes smoked per day , and alcohol intake with serum total IGF 1 and IGFBP 3 concentrations in 622 Black and 796 White male participants of the Coronary Artery Risk Development in Young Adults Study who were ages 20 to 34 years at the time of the first IGF measurement . ^^^ In generalized estimating equation analyses , IGF 1 decreased by 5 . 6 and 5 . 9 ng / mL per year increase in age for Black and White men , respectively ( P < 0 . 0001 ) , and there was an age related decline in IGFBP 3 that was stronger in Whites ( P < 0 . 0001 ) than Blacks ( P = 0 . 21 ) . ^^^ Average IGF 1 ( beta = 17 . 51 ng / mL ) and IGFBP 3 ( beta = 355 . 4 ng / mL ) levels across all three exams were lower in Blacks than Whites ( P < 0 . 0001 ) . ^^^ Increased BMI was associated with decreased IGF 1 ( P < 0 . 0002 ) , but was not associated with IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated whether alcohol ingestion altered IGF 1 or its major binding protein ( BP ) , IGFBP 3 , in a controlled feeding study in premenopausal women . ^^^ We also determined whether IGF 1 or IGFBP 3 was affected by menstrual cycle phase . ^^^ Although alcohol ingestion did not affect this increase , it significantly reduced IGF 1 concentrations at all phases ( 9 . 5 % ; P < 0 . 001 ) , whereas IGFBP 3 was unaffected by either menstrual phase or alcohol . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Measurement of bone age , bone mineral content ( BMC ) and bone density ( BD ) were performed and levels of serum gla protein ( BGP ) , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) were determined in girls with IPP at various stages of pubertal development . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
On the other hand , exogenous IGF 1 induced 8 11 % ( p < 0 . 05 ) increases in the proliferation , but cyclosporin A induced 30 80 % ( p < 0 . 05 0 . 01 ) reductions in the mRNA expression levels for endogenous IGF 1 , IGFR 1 , IGFBP 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 levels and IGF 1 / IGFBP 3 ratios increased with advancing pubertal stages , and maximum mean values were found at stages 3 4 in girls and at stage 4 in boys . ^^^ Serum IGF 1 and IGFBP 3 levels were significantly correlated with osteocalcin and bone specific alkaline phosphatase levels in boys , but not in girls . ^^^ This difference between the sexes , and the relation of the IGF 1 axis to increased bone formation during puberty in both sexes , can be explained by the rate of increase of the IGF 1 / IGFBP 3 ratio . ^^^ We conclude that the timing of the increased bone formation rate during puberty ; that is , the timing of the pubertal growth spurt , is determined by the maximum increase rate of the IGF 1 / IGFBP 3 ratio . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Concentrations of leptin , insulin , growth hormone , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS AND RESULTS : We assessed the association of circulating levels of IGF 1 and IGF binding protein 3 ( IGFBP 3 ) with early carotid atherosclerosis and atherosclerotic risk factors in 330 Japanese men ( age 51 . 6+ / 8 . 6 years , range 29 to 77 , body mass index [ BMI ] 23 . 6+ / 2 . 9 kg / m2 ) . ^^^ BMI and plasma insulin showed positive associations with circulating IGF 1 and IGFBP 3 . ^^^ IGF 1 and IGFBP 3 were associated with carotid IMT independent of age , BMI , blood pressure , and insulin . ^^^ However , it was not correlated with carotid IMT in the multivariate analyses which included IGF 1 or IGFBP 3 as a covariate . ^^^ CONCLUSIONS : Increased circulating IGF 1 and IGFBP 3 may be stimulators of atherosclerosis . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma IGF 1 , GH , insulin , and 41 kDa IGF binding protein ( putative salmon IGFBP 3 ) , and liver IGF 1 gene expression were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated GH , IGF 1 and IGFBP 3 concentrations in ten acromegalic patients before and after treatment with subcutaneous octreotide ( OCT sc ) and long acting octreotide ( OCT LAR ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Influence of iodine supplementation on serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) levels in severe iodine deficiency . ^^^ The negative impact of severe iodine deficiency ( SID ) on insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) was shown previously . ^^^ Height standard deviation scores ( HSDS ) , and serum levels of IGF 1 and IGFBP 3 were assessed both before and six months after a single dose of iodized oil . ^^^ IGF 1 and IGFBP 3 levels decreased significantly and FT 4 levels were suppressed six months after the supplementation , while TSH was normalized . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this cross sectional study , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and sex steroid levels ( estradiol in girls , testosterone in boys ) of 205 healthy adolescents ( 101 female , 104 male ) aged 9 17 years were measured . ^^^ Correlation of sex steroids with IGF 1 and IGFBP 3 during different pubertal stages . ^^^ Serum IGF 1 and IGFBP 3 levels differed significantly between pubertal developmental stages . ^^^ Serum IGF 1 levels and IGF 1 / IGFBP 3 ratios increased with proceeding stages and maximum mean values were found at stages 3 4 in girls and at stage 4 in boys . ^^^ Estradiol levels of girls and testosterone levels of boys differed significantly between stages , and in both sexes , serum IGF 1 levels and IGF 1 / IGFBP 3 ratios were significantly correlated with sex steroid levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ( P < 0 . 05 ) and IGFBP 3 ( P < 0 . 01 ) levels were also significantly lower than in controls . ^^^ Concomitantly , increased increments in IGF 1 and IGFBP 3 to a single bolus of GH were found ( P < 0 . 03 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Physical examinations ; serum leptin , IGF 1 , IGFBP 3 and IGFBP 1 levels ; and bone age 10 rays were performed annually for up to 48 months . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To 1 ) determine the onset and end of puberty as defined by serum insulin like growth factor ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) concentrations and 2 ) correlate articular epiphyseal cartilage complex structural changes with the onset and end of puberty . ^^^ METHODS : IGF 1 and IGFBP 3 were measured in serum samples from normal female and male horses age 9 715 days to determine peak and steady state values for horses transitioning through puberty . ^^^ POTENTIAL RELEVANCE : Defining the normal post natal to post pubescent concentrations for serum IGF 1 and serum IGFBP 3 establishes subsets of animals for age related studies and may be used to monitor horses for abnormally high IGF 1 concentrations due to natural disease or subsequent to systemic growth hormone administration . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To determine relationships between the concentrations of serum insulin like growth factor 1 ( IGF 1 ) and it ' s carrier protein insulin like growth factor binding protein 3 ( IGFBP 3 ) , as well as plasma erythropoietin ( EPO ) , and blood haemoglobin ( Hb ) in elderly subjects . ^^^ METHODS : Serum IGF 1 and IGFBP 3 and plasma EPO were , in addition to basal haematological tests , measured in a community based representative population sample of 70 yr olds ( n = 619 ; 317 women and 302 men ) . ^^^ RESULTS : Mean IGF 1 concentration was higher among men than women ( 155 vs . 138 mug / L , P = 0 . 0000 ) , and that of IGFBP 3 lower ( 2 . 21 vs . 2 . 65 mg / L , P = 0 . 0000 ) . ^^^ Hb concentration was positively correlated to concentrations of IGF 1 ( r = 0 . 15 , P < 0 . 01 for the men ; r = 0 . 34 , P < 0 . 001 for the women ) , and IGFBP 3 ( r = 0 . 07 , n . s . for the men ; r = 0 . 27 , P < 0 . 001 for the women ) , and negatively correlated to EPO concentration ( r = 0 . 34 , P < 0 . 001 for the men ; r = 0 . 42 , P < 0 . 001 for the women ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Clear cell RCC were characterized by significant increases in the mRNA expression of IGF 1 , IGFBP 3 and IGFBP 6 while papillary RCC exhibited down regulated expression of IGF 1 , IGFBP 4 and IGFBP 5 . ^^^ Semiquantitative assessment of immunohistochemical staining demonstrated significant increases in epithelial associated IGF 1 and IGFBP 3 in clear cell RCC , increased IGFBP 5 protein in papillary RCC and no significant changes in IGF / IGFBP protein expression in oncocytoma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We assayed plasma concentrations of estradiol , unconjugated estriol , testosterone , progesterone , prolactin , sex hormone binding globulin , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In premenopausal women , five of eight IGF 1 studies and four of six IGFBP 3 studies of circulating levels found that women in the highest quantile had more than twice the risk of developing breast cancer of those in the lowest , although in some this effect was only apparent at young ages . ^^^ Variation within the reference range of IGF 1 and IGFBP 3 may confer only modest increases in breast cancer risk , and any single polymorphism may only account for a small proportion of that variation . ^^^ Nevertheless , population attributable fractions for high circulating levels of IGF 1 and IGFBP 3 and for common genetic variants could be substantial . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate associations between diet and IGF 1 and IGFBP 3 levels in 7 to 8 year old children . ^^^ IGF 1 was positively associated with intakes of protein , magnesium , zinc , calcium , potassium , and phosphorus , and IGFBP 3 was positively associated with energy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of zinc supplementation on growth hormone secretion , IGF 1 , IGFBP 3 , somatomedin generation , alkaline phosphatase , osteocalcin and growth in prepubertal children with idiopathic short stature . ^^^ We investigated the effect of zinc supplementation on endogenous GH secretion , serum IGF 1 and IGFBP 3 concentrations , IGF 1 and IGFBP 3 generation in response to exogenous GH , bone formation markers , and linear growth of non zinc deficient children with idiopathic short stature . ^^^ We analyzed prospectively serum zinc , IGF 1 , IGFBP 3 , alkaline phosphatase , osteocalcin , and GH response to clonidine test , and performed a somatomedin generation test before and 6 weeks after zinc supplementation in 22 ( 16 M , 6 F ) prepubertal children with idiopathic short stature . ^^^ Percent change in IGF 1 and IGFBP 3 during the somatomedin generation test was not significantly affected by zinc supplementation ( 118 % vs 136 % and 57 % vs 44 % , respectively ) . ^^^ Zinc supplementation increased basal IGF 1 , IGFBP 3 , alkaline phosphatase and osteocalcin without changing GH response to clonidine . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 , and IGFBP 3 were measured in 162 children with acute lymphoblastic leukemia treated by the Berlin Frankfurt Munster Study Group . ^^^ At diagnosis we found elevated IGFBP 2 , low IGFBP 3 , low IGF 1 , and low normal IGF 2 , but normal IGFBP 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Data from 2 population based case control studies of colon and rectal cancer conducted in Utah and Northern California were used to evaluate associations between body mass index ( BMI ) , physical activity , energy intake and sucrose to fiber ratio and a CA repeat polymorphism of the IGF 1 gene , the A / C polymorphism at nucleotide 202 of the IGFBP 3 , the G972R polymorphism of the IRS 1 gene and the G1057D polymorphism of the IRS 2 gene . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have also shown that pre complexation of IGFBP with IGF 1 and 2 inhibits the subsequent association of IGFBP with heparin and conversely that heparin complexation of IGFBP 3 and 5 inhibits IGFBP binding to biosensor surfaces containing immobilised IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The principal regulator of the bioavailability of IGF 1 in the circulation is IGF binding protein 3 ( IGFBP 3 ) . ^^^ The aim of this study was to study factors associated with postnatal serum concentrations of IGF 1 and of IGFBP 3 in preterm infants from birth to an age corresponding to 40 wk postmenstruation . ^^^ We conducted a prospective , longitudinal study in which we measured serum IGF 1 and IGFBP 3 concentrations in 76 preterm infants from birth ( postmenstrual ages 23 32 wk ) until discharge from hospital around 40 wk . ^^^ Variables found to be associated with postnatal change over time of serum IGF 1 and IGFBP 3 were postmenstrual age ( p < 0 . 001 ) , weight gain ( standard deviation score ) ( p < 0 . 001 ) , and enteral intake of protein ( p < 0 . 001 ) . ^^^ The relationship between protein intake and IGF 1 ( and also between protein intake and IGFBP 3 ) was positive , as was the relationship between weight gain and IGF 1 ( and between weight gain and IGFBP 3 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
While serum IGF 1 levels were not changed by DHT treatment , IGF 1 gene expression levels increased and the mRNA levels of IGFBP 3 and IGFBP 5 were suppressed in the LA muscle after DHT replacement in castrated rats . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the chief binding protein for insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) . ^^^ Serum concentrations of IGFBP 3 are regulated by growth hormone ( GH ) and IGF 1 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Increased circulating insulin like growth factor 1 ( IGF 1 ) concentrations , frequently adjusted for IGF binding protein 3 ( IGFBP 3 ) , have been associated with increased risk of several types of cancer , including colon , prostate , and breast . ^^^ Studies have suggested that alcohol may affect IGF 1 or IGFBP 3 ; however , controlled feeding studies to assess alcohol ' s effects on IGF 1 or IGFBP 3 have not been conducted . ^^^ OBJECTIVE : To determine whether chronic , moderate alcohol intake affects serum IGF 1 or IGFBP 3 concentrations , we performed a controlled , crossover feeding study . ^^^ These findings suggest that the effect of alcohol intake should be considered in studies of IGF 1 , IGFBP 3 , and cancer in postmenopausal women . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although the response of plasma IGF 1 concentration to GHRP 2 administration was increased in the HP group ( P < 0 . 05 ) , there was no apparent effect of GHRP 2 treatment on plasma 38 43 kDa IGFBP 3 and 34 kDa IGFBP 2 at days 2 and 6 of treatment . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Cord plasma concentrations of IGF 1 and IGFBP 3 were lower in the babies of mothers who had smoked ( P = 0 . 02 and P = 0 . 01 , respectively ) . ^^^ Circulating concentrations of IGF 1 and IGFBP 3 along with measures of birth size but not markers of body fat are reduced , suggesting smoking results in a reduction in organ size and function . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition was determined by dual energy 10 ray absorptiometry ( DEXA ) and bioelectrical impedance spectroscopy ( BIS ) ; physical functioning by knee extensor strength ( KES ) and 30 s sit stand test ( SST ) ; and IGF 1 and IGFBP 3 in serum and muscle by radioimmunoassay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The colocalization of insulin like growth factor binding protein 3 ( IGFBP 3 ) and IGF 1 receptor ( IGF IR ) in the basal / germinative layer of the epidermis suggests a key role in modulating epidermal homeostasis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We found no differences in IGF 1 and IGFBP 3 serum levels , and the IGF 1 / IGFBP 3 ratio between the four groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 was measured in serum and hearts by an ELISA method , cardiac IGF 1 and IGF 2 receptors and IGFBP 3 were measured by Western blotting analysis , and mRNAs for cardiac IGF 1 and IGF 2 were measured by RT PCR . ^^^ Cu deficiency depressed serum and heart IGF 1 and heart IGFBP 3 protein levels and increased cardiac IGF 1 receptor protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ( CA ) 19 repeat and IGFBP 3 202 A / C genotypes and the risk of prostate cancer in Black and White men . ^^^ We investigated the relationship between the insulin like growth factor 1 ( IGF 1 ) cytosine adenine repeat ( CA ) ( 19 ) polymorphism located upstream of the gene ' s transcription start site , the insulin like growth factor binding protein 3 ( IGFBP 3 ) 202 A / C promoter region polymorphism , and prostate cancer risk in Black and White men . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , cases had higher levels of insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) and a higher molar ratio of IGFBP 3 to IGF 1 than did controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report a Chinese girl diagnosed with Laron syndrome at age 1 . 9 years with height 4 . 9 SDS , basal GH 344 mIU / ml , IGF 1 < 12 ng / ml , IGFBP 3 < 0 . 2 mg / ml , and undetectable GHBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
EXPERIMENTAL DESIGN : We measured the changes in total and free prostate specific antigen ( PSA ) and its association with insulin like growth factor 1 ( IGF 1 ) and IGFBP 3 levels after 1 year of treatment in 24 subjects given 4 HPR and 24 control subjects enrolled in a randomized bladder cancer prevention trial . ^^^ No association was observed between PSA levels and IGF 1 or IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A prospective study of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 and breast cancer risk . ^^^ Premenopausal women in the top vs bottom third of serum IGF 1 concentration had a nonsignificantly increased risk for breast cancer after adjustment for IGFBP 3 ( odds ratio ( OR ) 1 . 71 ; 95 % confidence interval ( CI ) : 0 . 74 3 . 95 ; test for linear trend , P=0 . 21 ) . ^^^ Serum IGFBP 3 was associated with a reduction in risk in premenopausal women after adjustment for IGF 1 ( top third vs the bottom third : OR 0 . 49 ; 95 % CI : 0 . 21 1 . 12 , P for trend=0 . 07 ) . ^^^ Neither IGF 1 nor IGFBP 3 was associated with risk in postmenopausal women and serum IGF 2 concentration was not associated with risk in pre or postmenopausal women . ^^^ These data are compatible with the hypothesis that premenopausal women with a relatively high circulating concentration of IGF 1 and low IGFBP 3 are at an increased risk of developing breast cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The low serum IGF 1 and IGFBP 3 concentrations in TM patients despite having normal GH reserve and serum GH binding protein levels suggest that a state of secondary GH insensitivity exists . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Age adjusted significant correlations were found between IGF 1 and smoking , fibrinogen , coffee consumption , lipoprotein ( a ) , osteocalcin and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It has been established that the concentrations of GH dependent peptides , such as insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) , are low in patients with GHD . ^^^ Therefore , levels of IGF 1 and IGFBP 3 are generally lower in short children compared with age matched norms . ^^^ The reported diagnostic value of sub normal basal levels of IGF 1 and IGFBP 3 is , in terms of sensitivity and specificity , approximately 70 % . ^^^ The Pfizer International Growth Database ( KIGS ) analysis of IGF 1 ( n = 2 , 750 ) and IGFBP 3 ( n = 1 , 300 ) levels in children with idiopathic GHD shows that these two parameters are now firmly embedded in diagnostic strategies around the world . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measurement of insulin like growth factor 1 ( IGF 1 ) and binding protein 3 ( IGFBP 3 ) levels can not always distinguish between healthy and GH deficient individuals . ^^^ In particular , IGFBP 3 as a marker of GH status is clearly less sensitive than IGF 1 and there is general agreement that its measurement does not provide useful diagnostic information . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similar results are observed for the serum IGF binding protein 3 ( IGFBP 3 ) response to GH , with a correlation between changes in serum concentrations of IGF 1 and changes in IGFBP 3 in normal , heterozygotic , GH insensitive and GH deficient participants . ^^^ In individuals with the E 180 splice mutation , IGF 1 and IGFBP 3 tests show sensitivity and specificity for detecting GH insensitivity ( GHI ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The insulin like growth factor 1 ( IGF 1 ) generation test has the potential to assess the ability of an individual to respond to an acute bolus of growth hormone ( GH ) , in terms of IGF 1 , IGF binding protein 3 and acid labile subunit responses . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Combined treatment caused a higher increase in IGFBP 1 and larger suppression of total and free IGF 1 , IGF 2 , and IGFBP 3 ( P < 0 . 01 for all ) , but less suppression of IGFBP 2 ( P < 0 . 05 ) compared with tamoxifen monotherapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured overnight fasting concentrations of GH sensitive insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) including GH binding protein ( GHBP ) , a marker of GH receptor sensitivity , in antiretroviral treated HIV infected patients with ( LIPO ) and without lipodystrophy ( NONLIPO ) and antiretroviral naive HIV infected patients ( NAIVE ) . ^^^ IGF 1 and IGFBP 3 were increased in LIPO compared with NONLIPO ( p < 0 . 05 ) , but did not differ significantly between NONLIPO and NAIVE . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The authors evaluated the prognostic information of IGF 1 , IGFBP 3 , leptin , and prealbumin in sera sampled at diagnosis from 256 consecutive patients with renal cell carcinoma . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of IGF 1 , free IGF , IGFBP 3 , and IGFBP 1 were measured in blood samples collected in 1989 to 1990 . ^^^ Findings : Among postmenopausal women , neither IGF 1 , IGFBP 3 , IGFBP 1 , nor free IGF was associated with breast cancer risk [ RRs , top versus bottom quintile : IGF 1 , 1 . 0 ; 95 % confidence interval ( 95 % CI ) , 0 . 7 1 . 4 ; IGFBP 3 , 0 . 8 ; 95 % CI , 0 . 6 1 . 1 ; IGFBP 1 , 0 . 9 ; 95 % CI , 0 . 6 1 . 5 ; and free IGF , 1 . 0 ; 95 % CI , 0 . 6 1 . 4 ] . ^^^ Higher IGF 1 plasma levels , however , were associated with a modestly elevated breast cancer risk ( RR , 1 . 6 ; 95 % CI , 1 . 0 2 . 6 ) among the premenopausal women , with a stronger association among premenopausal women ages < or =50 ( RR , 2 . 5 ; 95 % CI , 1 . 4 4 . 3 ) ; further adjustment for IGFBP 3 did not greatly change these estimates . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At 32 hours , simulating refrigerated storage and overnight shipping , there was a 2 . 9 % decrease ( 95 % confidence interval , 0 . 7 5 . 1 ) in insulin like growth factor 1 ( IGF 1 ) but no significant change in carotenoids , tocopherols , testosterone , 3alpha androstanediol glucuronide ( AAG ) , sex hormone binding globulin ( SHBG ) or insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ A 144 hour processing delay , simulating weekend blood collection or shipping delay , resulted in significant changes in gamma tocopherol ( 1 . 5 % ) , IGF 1 ( 5 . 7 % ) , IGFBP 3 ( 2 . 9 % ) , SHBG ( 4 . 0 % ) , testosterone ( +4 . 7 % ) , and AAG ( +5 . 5 % ) . ^^^ The rank order and intraclass correlations between analytes from blood processed immediately and those subjected to delayed processing were 0 . 96 or higher for carotenoids , tocopherols , AAG , and SHBG , and between 0 . 87 and 0 . 95 for IGF 1 , IGFBP 3 , and testosterone . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Early developmental changes in IGF 1 , IGF 2 , IGF binding protein 1 , and IGF binding protein 3 concentration in the cerebrospinal fluid of children . ^^^ Herein , we have determined the concentrations of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 in cerebral spinal fluid ( CSF ) samples that were collected from children who were 1 wk to 18 y of age . ^^^ The concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 in CSF from children < 6 mo of age were significantly higher than in older children , whereas IGF 2 was higher in the older group . ^^^ This is in contrast to what is observed in the peripheral circulation , where IGF 1 and IGFBP 3 are low at birth and rise rapidly during the first year , reaching peak levels during puberty . ^^^ Higher concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 in the CSF of very young children suggest that these proteins might participate in the active processes of myelination and synapse formation in the developing nervous system . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Putative role of serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) levels in the development of prostate cancer in Arab men . ^^^ The purpose of this study was to determine serum IGF 1 and IGFBP 3 levels in healthy Arab men and in Arab men with newly diagnosed benign prostatic hyperplasia ( BPH ) and prostate cancer , and to compare these values with values reported in Caucasians . ^^^ The serum concentrations of IGF 1 and IGFBP 3 were determined using Immunoradiometric assay ( IRMA ) kits . ^^^ RESULTS : As in Caucasians , serum IGF 1 and IGFBP 3 levels declined with age in Arab men . ^^^ Arab men with newly diagnosed prostate cancer had significantly higher serum IGF 1 level ( P < 0 . 01 ) and lower IGFBP 3 levels ( P < 0 . 01 ) compared to age matched Arabs without the disease . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the human body , IGF 1 circulates in the blood bound to a binding protein 3 ( IGFBP 3 ) , which regulates the delivery of IGF 1 to target tissues , and particular proteases clip them apart in response to stresses and release IGF 1 as needed . ^^^ For the treatment of IGF 1 deficiency , it is desirable to administer IGF 1 bound to IGFBP 3 to maintain the normal equilibrium of these proteins in the blood . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Height , weight , IGF 1 , IGFBP 3 and thyroid functions in prepubertal children with attention deficit hyperactivity disorder : effect of methylphenidate treatment . ^^^ Height SDS , weight SDS , BMI SDS , serum GH , IGF 1 , IGFBP 3 , T 4 , free T 4 , T 3 , and TSH were measured at each visit . ^^^ Methylphenidate treatment did not significantly affect SDS of height , weight , BMI , IGF 1 and IGFBP 3 in the long run . ^^^ CONCLUSIONS : Prepubertal children with ADHD had normal height , weight , BMI , serum IGF 1 and IGFBP 3 and thyroid functions . ^^^ Methylphenidate treatment had no sustained effects on growth parameters , IGF 1 and IGFBP 3 during the follow up period of this study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conducted a randomized trial to determine the effects of 2 dietary patterns on serum IGF 1 , IGFBP 1 and IGFBP 3 in free living premenopausal women : phase 1 , an isocaloric low fat , high fiber ( LFHF ) vs . usual diet , and phase 2 , a soy supplement either with or without isoflavones ( soy+IF vs . soy IF ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum concentrations of IGF 1 , IGFBP 3 , insulin , cystatin C , creatinine , endogenous creatinine clearance and HbA1c levels were assessed by routine laboratory methods ; serum IGF 1 bioactivity was estimated by a highly specific kinase receptor activation assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Lack of synergy between estrogen and progesterone on local IGF 1 , IGFBP 3 and IGFBP 2 secretion by both hormone dependent and hormone independent breast cancer explants in vitro . ^^^ The aim of the present study was to investigate direct effects of estrogen ( E 2 ) or progesterone ( P 4 ) given separately vs . estrogen+progesterone on local IGF 1 , IGFBP 3 and IGFBP 2 secretion . ^^^ In hormone dependent and hormone independent tissues , treatment with estrogen+progesterone increased IGF 1 and IGFBP 2 secretion with concomitant decrease in IGFBP 3 , in the same manner as E 2 or P 4 given alone . ^^^ RU 486 decreased the P 4 and E2+P4 stimulated IGF 1 secretion with parallel stimulation of IGFBP 3 secretion by ER+ / PR+ explants . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , the PI 3 kinase inhibitor , LY 294002 , upregulates IGFBP 3 expression but downregulates IGF 1 and IGF 2 , indicating that PTEN controls IGFBP 3 and IGFs by an Akt dependent pathway . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We present experimental results using SPR for the interaction of insulin like growth factor 1 ( IGF 1 ) with one of its binding proteins , IGF binding protein 3 ( IGFBP 3 ) , and show that the dissociation , even with the addition of soluble heparin in the dissociation phase , does not exhibit the expected exponential decay characteristic of a 1 : 1 binding reaction . ^^^ Our analysis of the IGF 1 IGFBP 3 data indicates that rebinding is prominent for this system and that the theoretical predictions fit the experimental data well . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
After assessment of fasting plasma GH , IGF 1 and IGF binding protein 3 ( IGFBP 3 ) , GHRH ( 100 microg ) and GHRP 6 ( 93 microg ) were given intravenously as a bolus injection . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) are measured to diagnose disorders of the somatotropic axis in children and adults . ^^^ In clinical studies samples for IGF 1 and IGFBP 3 measurement must often be stored for months and sent to specialized laboratories . ^^^ Therefore , we tested the stability of IGF 1 and IGFBP 3 in whole blood , serum and plasma from 12 volunteers at 4 degrees , 22 degrees , and 37 degrees C for several hours and at 25 degrees C for several months . ^^^ The effect of only one protease inhibitor ( Aprotinin = Trasylol , Bayer , Germany ) on IGF 1 and IGFBP 3 measured in the automated IMMULITE assay system ( DPC , Los Angeles ) was tested . ^^^ IGF 1 and IGFBP 3 were stable in heparinized whole blood , plasma and serum at 22 degrees C up to 24 hours . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : Insulin like growth factor 1 ( IGF 1 ) is recognized as a potent mitogen for many cancer cell lines and there is good evidence that lung cancer cells produce both IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ The aim of this study was to investigate the clinical significance of IGF 1 and IGFBP 3 levels in serum and in bronchoalveolar lavage ( BAL ) fluid by comparing lung cancer patients with healthy controls . ^^^ METHODS : BAL fluid and serum samples were obtained from 24 lung cancer patients and 12 healthy controls , and were analyzed for IGF 1 and IGFBP 3 levels by a two site immunoradiometric assay . ^^^ RESULTS : Serum IGF 1 and IGFBP 3 levels were lower in lung cancer patients , but the difference between the groups did not reach a statistical significance . ^^^ IGF 1 / IGFBP 3 ratio in ELF was significantly lower in lung cancer patients ( P=0 . 035 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The IGF 1 to IGFBP 3 molar ratio was associated with colon cancer risk ( MVRR , 2 . 82 ; 95 % CI , 1 . 35 5 . 88 ) , and women with low levels of both IGF I / IGFBP 3 and C peptide ( or high IGFBP 1 ) were at low risk , and elevation of either was sufficient to increase risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Preoperative sera from 141 patients undergoing RRP were analyzed for IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 . ^^^ Average levels of IGF 1 , IGFBP 1 , IGFBP 2 and IGFBP 3 were 156 . 5 ng / ml , 48 . 1 ng / ml , 396 . 8 ng / ml and 3303 . 7 ng / ml , respectively . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENT : The subject , from a consanguineous pedigree , had circulating levels of GH and GH binding protein that were normal to elevated , whereas IGF 1 ( 7 . 2 ng / ml ; normal , 242 600 ) , IGF binding protein 3 ( 543 ng / ml ; normal , 2500 4800 ) , and acid labile subunit ( 1 . 22 microg / ml ; normal , 5 . 6 16 ) levels were abnormally low and failed to increase during an IGF 1 generation test . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have studied effects of feeding vitamin A ( 550 , 000 IU / d ) that exceed daily requirements about 8 fold for up to 2 mo to dairy cows during the dry period on concentrations of retinol and its metabolites in plasma and milk , milk lactoferrin , plasma and milk IGF 1 and IGFBP 3 , lactoferrin and IGF 1 mRNA levels in mammary gland tissue , mammary gland apoptosis , and 100 d milk yield in the ensuing lactation . ^^^ In conclusion , supplementation of dairy cows during the dry period with high amounts of vitamin A did not significantly modify concentrations of lactoferrin , IGFBP 3 , and IGF 1 in plasma and in mammary secretions , but slightly decreased energy corrected 100 d milk yield and milk fat yield , possibly because of enhanced apoptic rates of mammary cells . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , 2 and IGFBP 3 were lower in the patients than in CS ( p = 0 . 03 , 0 . 02 and 0 . 001 , respectively ) with a tendency to increase up to day 7 . ^^^ These findings indicate that critically ill children are characterized by low levels of IGF 1 and 2 as well as IGFBP 3 accompanied by elevated levels of GH , probably reflecting the development of peripheral GH resistance . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In growth plate chondrocytes in primary culture , incubation with IGF 1 increased the concentrations of IGFBP 3 and IGFBP 5 in conditioned cell culture medium in a dose and time dependent manner . ^^^ Coincubation of IGF 1 with specific inhibitors of the p42 / 44 MAPK pathway ( PD 098059 or U 0126 ) completely abolished the stimulatory effect of IGF 1 on IGFBP 3 mRNA expression but did not affect increased IGFBP 5 mRNA levels . ^^^ In contrast , inhibition of the phosphatidylinositol 3 kinase signaling pathway by LY 294002 abrogated both IGF 1 stimulated IGFBP 3 and 5 mRNA expression . ^^^ These data suggest that IGF 1 modulates its activity in cultured rat growth plate chondrocytes by the synthesis of both inhibitory ( IGFBP 3 ) and stimulatory ( IGFBP 5 ) binding proteins . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Females had significantly higher levels of IGF 1 , IGFBP 3 , growth hormone , and insulin when compared with males , P < 0 . 05 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 levels were also measured at baseline . ^^^ Thirty three per cent of subjects had normal IGF 1 ; neither IGF 1 nor IGFBP 3 standard deviation scores ( SDS ) correlated with ITT results . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OUTCOME MEASURES : Endocrine [ GH , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , GH binding protein , and SHBG ] and metabolic ( fat oxidation ) end points were used as outcome measures . ^^^ The effects of E 2 and raloxifene on IGF 1 , IGFBP 3 , IGF I / IGFBP 3 molar ratio , GH binding protein , and SHBG were significantly different ( P < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The circulating levels of IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 did not differ between patients and controls . ^^^ The subgroup of patients receiving hydrocortisone ( HC ) replacement ( n=24 ) had significantly lower levels of androgens ( suppressed by 80 100 % ) as well as IGF 1 and IGFBP 3 as compared with the patients not receiving HC . ^^^ Estrogen discontinuation induced an increase in IGF 1 ( 167+ / 15 vs 206+ / 14 microg / l , P=0 . 005 and IGFBP 3 ( 3887+ / 139 vs 4309+ / 138 microg / l , P=0 . 0005 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Understanding IGF 1 chondroinductive and IGFBP 3 chondroinhibitory effects would provide critical information to optimize the therapeutic use of MSC in cartilage disorders . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 assessment in the management of childhood onset growth hormone deficiency . ^^^ Due to their GH dependency and relative stability in circulation , IGF 1 and IGFBP 3 serum concentrations were proposed as reliable indicators of daily GH secretion . ^^^ However , the sensitivity of assays for both IGF 1 and IGFBP 3 is inadequate to exclude the diagnosis of GHD merely on the basis of a normal value of the two parameters , although it seems likely that IGF 1 values higher than 1 SD reflect a normal GH secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 and ALS are GH dependent peptides and their production is disturbed in states of GH insensitivity . ^^^ This chapter explores the relative degrees of IGF 1 , IGFBP 3 and ALS deficiency across the spectrum of GH insensitivity . ^^^ In classical GH insensitivity syndrome ( GHIS ) , known as Laron syndrome , due to GH receptor ( GHR ) deficiency , serum IGF 1 , IGFBP 3 and ALS are severely reduced with inability to produce these peptides during an IGF 1 generation test . ^^^ In these cases the IGF 1 , IGFBP 3 deficiency is less severe . ^^^ IGF 1 , IGFBP 3 and ALS were significantly higher ( p < 0 . 05 ) in pubertal compared with pre pubertal subjects in the same series . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating levels of insulin like growth factor 1 ( IGF 1 ) and its principal binding protein IGFBP 3 are reduced , whereas those of the inhibitory binding protein , IGFBP 1 , tend to be high in children and adolescents with type 1 diabetes mellitus ( T1DM ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : To assess the degradation of frozen serum components in the JACC Study , we compared levels of various components ( IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , sFas , and total SOD activity ) between fresh and stored sera collected from other inhabitants . ^^^ Serum levels of constituents were measured by immunoradiometric assay ( IGF 1 , IGF 2 and IGFBP 3 ) , quantitative enzyme immunoassay ( TGF beta 1 ) , enzyme linked immuno adsorbent assay ( sFas ) , and an improved nitrite method ( SOD activity ) . ^^^ Levels of IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 and sFas in sera after storage for 9 years at 80 degrees C were similar to those of fresh sera newly collected from inhabitants . ^^^ The distributions of serum IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years . ^^^ CONCLUSIONS : There was no statistically significant difference in distribution of measured values of IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , and sFas between newly collected sera and frozen specimens stored for 9 years . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The expression of IGFBP 3 , 4 , and 5 was examined using immunohistochemistry and in situ hybridization , and IGF 1 and IGF 1 receptors were also immunohistochemically detected . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Height , weight as well as insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) were measured during the preoperative period , 6 months and 1 year after surgery . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The variable peak IGF 1 response is attributable in part to IGFBP 3 differences across this pediatric age range . ^^^ Models of rhIGF 1 dosing based upon body surface area ( BSA ) or initial IGFBP 3 resulted in predictable peak serum IGF 1 levels ( r = 0 . 78 ; p < 0 . 03 ) . ^^^ IGFBP 3 product correlated closely with peak IGF 1 level ( r = 0 . 85 ; p < 0 . 007 ) . ^^^ Considering BSA and serum IGFBP 3 concentration in children is appropriate for subcutaneous IGF 1 administration . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We demonstrated that the level of IGF 1 and ALS mRNAs is dramatically decreased in both young and aged dwarf livers , transcripts encoding IGF IR and IGFBP 1 are elevated in young dwarfs , but normalize to control levels in aged dwarf livers while transcripts encoding IGFBP 3 are elevated only in aged controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To evaluate the role of IGFs in colorectal cancer , we examined the relationship of two common genetic polymorphisms in IGF 1 ( a cytosine adenosine dinucleotide repeat ) and IGFBP 3 ( a G > C single nucleotide polymorphism ) with colorectal cancer risk , as well as their potential modification by physical activity , body mass index ( BMI ) , and postmenopausal hormone use . ^^^ Colorectal cancer was modestly associated with having an IGF 1 genotype other than homozygous for 19 repeats ( odds ratio , 1 . 3 ; 95 % confidence interval , 1 . 0 1 . 6 ) and having the GG IGFBP 3 genotype ( odds ratio , 1 . 3 ; 95 % confidence interval , 1 . 0 1 . 8 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured serum IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) prior to prostate biopsy and compared them between 103 men with HGPIN ( cases ) and 205 men with normal prostate histology ( controls ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Laboratory and seroepidemiologic studies have suggested that insulin like growth factor binding protein 3 ( IGFBP 3 ) , the main binding protein for IGF 1 , may be protective against colorectal cancer . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IL 1beta , IL 6 , TNF alpha , IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , TSH , fT 3 and fT 4 were measured using standard commercial kits . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum GH , IGF 1 , IGFBP 1 and IGFBP 3 were measured by ELISA . ^^^ The increase of serum IGF 1 and IGFBP 3 after rhGH treatment was also observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma active ghrelin correlated positively with IGFBP 3 ( p=0 . 005 ) but negatively with total ghrelin and free IGF 1 ( p=0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the study was to test the hypothesis that in adolescents with type 1 diabetes mellitus and microalbuminuria ( MA ) there are specific disorders of serum insulin like growth factor 1 ( IGF 1 ) and concentrations of its binding proteins , IGFBP 1 and IGFBP 3 , that could be of importance in the pathogenesis of microvascular diabetic complications . ^^^ Serum fasting concentrations of IGF 1 and overnight urine albumin concentrations were measured by radioimmunoassay , IGFBP 1 and IGFBP 3 concentrations by immunoradiometric assay and HbA1c by high performance liquid chromatography methods . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Differentiation between growth hormone deficiency ( GHD ) and idiopathic short stature ( ISS ) based on GH tests and basal IGF 1 and IGFBP 3 levels may be difficult . ^^^ The aim of this study was to evaluate the role of pharmacological GH tests , IGF 1 and IGFBP 3 generation test and height velocity off treatment in the evaluation of GHD and ISS . ^^^ The generation test was performed concomitantly ( 0 . 1 IU / kg GH s . c . for 4 days ) with IGF 1 and IGFBP 3 measured on the 4th day in both groups . ^^^ In the ISS group , deltaIGF 1 and deltaIGFBP 3 responses were low in 31 % and 7 % , respectively , and the correlation between basal IGF 1 , IGFBP 3 and HV and between delta values in the generation test were significantly positive , pointing to a difference in the growth response of these children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Dexamethasone administration attenuates the inhibitory effect of lipopolysaccharide on IGF 1 and IGF binding protein 3 in adult rats . ^^^ The aim of this study was to investigate whether glucocorticoid administration had a beneficial effect on serum concentrations of insulin like growth factor 1 ( IGF 1 ) and on IGF binding protein 3 ( IGFBP 3 ) in rats injected with lipopolysaccharide ( LPS ) . ^^^ In conclusion , dexamethasone pretreatment partially attenuates the inhibitory effect of LPS on serum IGF 1 by blocking the decrease of its gene expression in the kidney as well as by attenuating the decrease in serum concentrations of IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Due to their essential roles in development we examined levels of insulin like growth factor 1 ( IGF 1 ) as well as its main binding protein ( IGFBP 3 ) in a group of AT patients . ^^^ Serum levels of IGF 1 were found to be below the 3rd percentile in 9 ( 56 % ) out of 16 patients and of IGFBP 3 in 13 ( 81 % ) out of 16 patients . ^^^ Our observations demonstrate that IGF 1 and IGFBP 3 levels reflect the impaired growth status in patients with AT . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Association between dynamic exercise therapy and IGF 1 and IGFBP 3 concentrations in the patients with rheumatoid arthritis . ^^^ We aimed to evaluate the relationship between short term dynamic exercise therapy and insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels in rheumatoid arthritis ( RA ) patients . ^^^ Morning stiffness , pain ( VAS ) , Health assessment questionnaire ( HAQ ) and Ritchie articular index ( RAI ) were evaluated and erythrocyte sedimentation rate , serum C reactive protein , IGF 1 and IGFBP 3 levels of the participants were recorded . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma concentrations of IGF 1 , insulin like growth factor binding protein 3 , serum bone alkaline phosphatase , and urinary N telopeptide collagen crosslink ( NTx ) concentrations were measured at 0 , 3 , and 6 mo after 24 h without exercise and a 12 h fast . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the association of polymorphisms in IGF 1 and IGFBP 3 and circulating levels of IGF 1 and IGFBP 3 in 323 population based control subjects enrolled in a case control study of colorectal cancer from September 1999 through February 2002 . ^^^ Total IGF 1 and IGFBP 3 levels were measured using ELISA assays , and all subjects were genotyped for a microsatellite polymorphism in IGF 1 and a single nucleotide polymorphism in IGFBP 3 . ^^^ Having an IGF 1 genotype other than homozygous for the 19 repeat allele was associated with higher IGFBP 3 levels ( 1 , 945 versus 2 , 052 ng / mL ) . ^^^ Furthermore , both IGF 1 and IGFBP 3 genotypes modified the relationship between postmenopausal hormone use and IGFs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated associations of having been breastfed with serum IGF 1 and IGFBP 3 in childhood . ^^^ In models also controlling for birthweight , gestational age , mother ' s age , and socioeconomic and dietary factors , the breastfeeding IGF 1 association was attenuated ( regression coefficient : 3 . 3 ng / ml ; 4 . 2 10 . 7 ; P = 0 . 4 ) ; further adjustment for IGFBP 3 made little difference ( regression coefficient : 4 . 1 ng / ml ; 2 . 8 10 . 9 ; P = 0 . 2 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Infection with the human immunodeficiency virus ( HIV ) is often associated with a decrease of the concentrations of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and an increase of IGFBP 1 and 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 ( Insulin like growth factor 1 ) is a growth factor whose biological activity at cellular level is regulated by 6 binding proteins ( IGFBs ) ; one of them , IGFBP 3 , is the most abundant in blood . ^^^ Both IGF 1 and IGFBP 3 are expressed by bone cells and may be important factors in bone remodelling ( 1 5 ) . ^^^ The aims of this study were to evaluate the presence of IGF 1 and IGFBP 3 in crevicular fluid and to assess whether their levels changed during orthodontic tooth movement in patients under orthodontic treatment . ^^^ Changes in the amounts of IGF 1 and IGFBP 3 in crevicular fluid may reflect changes in alveolar bone remodelling . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSION : Both raloxifene and oral testosterone increased serum testosterone , but raloxifene significantly decreased serum IGF 1 levels without affecting IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 has very high affinity for IGFs ( k ( d ) approximately 10 ( 10 ) M ) , it transports > 75 % of serum IGF 1 and 2 , whereas it ' s affinity for insulin is very low . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 levels ( 2 . 03 + / 0 . 69 vs . 1 . 13 + / 0 . 89 SDS ; P = 0 . 005 ) and IGFBP 3 levels ( 0 . 92 + / 1 . 26 vs . 0 . 40 + / 1 . 08 SDS ; P = 0 . 006 ) were significantly lower in the mut+ group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The analysis revealed that the pleiotropic genetic effects were significant for 2 pairs of traits , namely for IGF 1 and IGF BP 3 , and for IGF BP 1 and leptin . ^^^ The common environmental factors were consistently a significant source of correlation between all pairs ( barring IGF 1 and leptin ) of the studied molecules ; they were the sole predictors of correlation between IGF 1 and IGF BP 1 , and between IGF BP 1 and IGF BP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum Zn , IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , serum insulin and glucose , tissue Zn and jejunal sucrase activity were measured . ^^^ Relative liver IGF 1 and IGFBP 3 mRNA levels were quantified . ^^^ Liver IGF 1 and IGFBP 3 mRNA levels were higher in low Zn animals compared with controls . ^^^ Serum IGF 1 and IGFBP 3 levels were not affected by diet . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Serum concentrations of IGF 1 , IGF binding protein ( IGFBP ) 3 , and their molar ratio ( IGF I / IGFBP 3 ) were measured with radioimmunoassay kits in stored blood samples from the participants of an original chemoprevention trial . ^^^ The serum concentrations of IGF 1 and IGF I / IGFBP 3 at baseline were significantly lower in female than in male participants . ^^^ After 3 months of treatment , the serum level of IGF 1 and IGF I / IGFBP 3 were significantly lower in the 9 cis RA group than in the placebo group ( P = . 03 and P < . 01 , respectively ) , but the IGFBP 3 level was significantly higher ( P = . 03 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition from dual energy 10 ray absorptiometry ( DXA ) and serum IGF 1 , IGF binding protein 3 ( IGFBP 3 ) and lipid levels were determined centrally . ^^^ Dosing patterns in the two groups were paralleled by the changes in IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum total calcium , phosphorus , and intact parathyroid hormone ( PTH ) levels were measured , thyroid function test was performed , and serum IGF 1 and IGFBP 3 levels were also estimated . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
For example , in heifers treated with growth hormone or estrogen , expression of IGF 1 binding proteins ( IGFBP 3 ) protein was reduced , thus effecting an increase in free IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High serum concentrations of insulin like growth factor 1 ( IGF 1 ) are associated with an increased risk of breast , prostate , colorectal , and lung cancer whereas IGF binding protein 3 ( IGFBP 3 ) seems to exert a protective effect . ^^^ Serum levels of IGF 1 and IGFBP 3 during adjuvant chemotherapy for primary breast cancer . ^^^ Therefore , patients may benefit from low IGF 1 levels and high IGFBP 3 levels . ^^^ This study evaluated whether adjuvant anthracycline containing chemotherapy modulates IGF 1 and / or IGFBP 3 serum levels in breast cancer patients . ^^^ In 18 patients undergoing adjuvant treatment for primary breast cancer , IGF 1 and IGFBP 3 serum levels were measured with immunoassays during chemotherapy regimens of either 5 fluorouracil , epirubicin and cyclophosphamide ( FEC ) or epirubicin and cyclophosphamide ( EC ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Neither tissue microdialysate nor serum concentrations of IGF 1 and IGF binding proteins ( IGFBP 3 and IGFBP 4 ) or procollagen type 1 N terminal propeptide changed from resting values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A reciprocal regulation of IGFBP 3 and 5 by IGF 1 was observed between the two cell types . ^^^ IGF 1 induced large dose dependent increases in IGFBP 3 mRNA and protein levels in BME cells , while IGFBP 5 protein was barely detectable and mRNA levels were detectable only by qRT PCR . ^^^ In BMFs , IGF 1 induced large increases in IGFBP 5 mRNA and protein while IGFBP 3 mRNA was only slightly increased by IGF 1 treatment and the protein was difficult to detect . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Frequent sampling for total IGF 1 , free IGF 1 and IGFBP 3 was performed on days 0 , 1 , 7 , 14 and 15 . ^^^ Both the time to maximum concentration ( Tmax ) and the maximum free IGF 1 levels ( Cmax ) on day 1 for all groups were substantially less than previously published studies , wherein lower doses of rhIGF 1 were given without IGFBP 3 . ^^^ The frequency of side effects such as edema , jaw pain and arthralgias was 4 % which is less than that has been reported in previous studies wherein IGF 1 was administered without IGFBP 3 . ^^^ Coadministration of IGFBP 3 with IGF 1 produces lower free IGF 1 ( Tmax and Cmax ) levels compared to administration of IGF 1 alone and is associated with relatively low incidence of side effects during 2 weeks of administration . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Expression of albumin , IGF 1 , IGFBP 3 in tumor tissues and adjacent non tumor tissues of hepatocellular carcinoma patients with cirrhosis . ^^^ ALB mRNA , IGF 1 mRNA , and IGFBP 3 mRNA in liver tissues ( including tumor tissues and adjacent non tumor tissues ) were detected by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ RESULTS : In HCC patients with cirrhosis , hepatic ALB mRNA , IGF 1 mRNA , and IGFBP 3 mRNA of tumor tissues or adjacent non tumor tissues were lower than the normal liver tissues , while in tumor tissues , hepatic ALB mRNA and IGFBP 3 mRNA were lower , hepatic IGF 1 mRNA was higher than in adjacent non tumor tissues . ^^^ CONCLUSION : Imbalance of IGF 1 and IGFBP 3 may play a role in hepatocarcinogenesis and tumor development of liver cirrhosis patients . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
No treatment effect was found for muscle IGF 1 ( P = 0 . 36 ) , IGF 2 ( P = 0 . 51 ) , IGFBP 3 ( P = 0 . 70 ) , or IGFBP 5 ( P = 0 . 51 ) mRNA abundance . ^^^ The abundance of IGF 1 ( P = 0 . 72 ) , IGF 2 ( P = 0 . 34 ) , and IGFBP 3 ( P = 0 . 99 ) in hepatic tissue was not influenced by treatment . ^^^ Uterine IGF 1 ( P = 0 . 46 ) , IGF 2 ( P = 0 . 40 ) , IGFBP 3 ( P = 0 . 29 ) , and IGFBP 5 ( P = 0 . 35 ) mRNA abundance did not differ between treatments . ^^^ Placental IGF 1 ( P = 0 . 30 ) , IGF 2 ( P = 0 . 18 ) , IGFBP 3 ( P = 0 . 94 ) , and IGFBP 5 ( P = 0 . 42 ) mRNA abundance did not differ between treatments . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 proteolysis was measured by an in vitro proteolytic activity assay , IGF 1 bioactivity by novel IGF 1 kinase receptor activation assay ( KIRA ) and other hormones by immunoassay . ^^^ No change in IGFBP 3 proteolysis or IGF 1 bioactivity was found during exercise or recovery in either group . ^^^ CONCLUSION : Submaximal exercise induced minor changes in IGFBP 1 , 2 and 3 , without affecting IGFBP 3 proteolysis and IGF 1 bioavailability . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Measures at baseline , 6 and 12 months included body weight , height , body fat , lean mass , body fat % , and waist circumference , as well as fasting glucose , insulin , insulin resistance , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body size measurements were taken by trained interviewers ; genotypes were determined for the IGF 1 CA repeat , the IGFBP 3 202 C > A substitution , the IRS 1 G972R and IRS 2 G1057D substitutions , and the vitamin D receptor ( VDR ) BsmI and FokI polymorphisms . ^^^ Two associations were observed that were consistent in both Hispanics and non Hispanic Whites : IGF 1 CA repeat alleles of length other than 19 were associated with higher mean waist to hip ratios ( WHR ) , P = 0 . 01 , and women who carried an IGFBP 3 A allele , compared with women with the CC genotype , more often reported high birthweight ( odds ratio , 1 . 9 ; 95 % confidence interval , 1 . 1 3 . 2 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor 1 ( IGF 1 ) and its major binding protein IGF binding protein 3 ( IGFBP 3 ) have been associated with increased risk of premenopausal breast cancer , although risk estimates varied broadly . ^^^ An extension of a case control study ( 138 cases , 259 matched controls ) on IGF 1 and breast cancer in premenopausal women nested in the New York University Women ' s Health Study cohort offered the opportunity to address the hypothesis that such variability may have been the result of variations in the ability of different IGFBP 3 assays to specifically measure intact / functional forms of the protein . ^^^ IGF 1 and IGFBP 3 had originally been measured using in house RIAs . ^^^ IGF 1 and IGFBP 3 measurements by the DSL ELISA methods showed statistically significant relationships with risk . ^^^ The association with IGF 1 became substantially weaker and lost statistical significance after adjustment for IGFBP 3 using DSL ELISA , but became considerably stronger when adjusting for the functional IGFBP 3 measurements [ OR = 2 . 43 ( 1 . 21 4 . 90 ) ; P = 0 . 005 ] , or when considering the molar ratio of IGF 1 to IGFBP 3 [ OR = 2 . 37 ( 1 . 13 5 . 00 ) ; P = 0 . 02 ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND METHODS : Fasting serum IGF 1 , IGFBP 3 , and insulin concentrations were measured , and their determinants were analyzed in a cohort of young adult subjects ( 22 yr of age ) born either small ( SGA ; n = 461 ) or appropriate ( AGA ; n = 568 ) for gestational age . ^^^ RESULTS : In adulthood , subjects born SGA had significantly lower mean serum IGF 1 ( 320 + / 137 vs . 348 + / 143 microg / liter ; P = 0 . 0015 ) , IGFBP 3 ( 4700 + / 700 vs . 4800 + / 800 microg / liter ; P = 0 . 04 ) , and IGF I / IGFBP 3 ratio ( 0 . 067 + / 0 . 026 vs . 0 . 072 + / 0 . 025 ; P = 0 . 01 ) than those born AGA . ^^^ The fasting IGF 1 concentration and the IGF I / IGFBP 3 ratio were significantly inversely associated with age , body mass index , smoking , and oral contraception and were positively related to birth weight and fasting insulin levels . ^^^ After adjustment for age , height , body mass index , gender , smoking , and oral contraception , the mean IGF 1 concentration and the mean IGF I / IGFBP 3 ratio remained significantly lower in the SGA compared with the AGA group ( P = 0 . 003 and P = 0 . 01 , respectively ) . ^^^ CONCLUSIONS : Serum IGF 1 concentrations and the IGF I / IGFBP 3 ratio are lower in adult subjects born SGA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DOTAP / Chol increased IGF 1 and KGF protein concentration and caused concomitant cellular responses , for example , by increasing IGFBP 3 , P < 0 . 05 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We performed anthropometry and measured fasting plasma insulin , IGF 1 , insulin like growth factor binding protein ( IGFBP ) 1 and IGFBP 3 . ^^^ Conversely , fasting insulin , IGFBP 3 and IGF 1 were all higher in UK Gujaratis ( mean IGF 1 145 . 9 [ 138 . 1 153 . 6 ] ng / ml in UK Gujaratis and 100 . 9 [ 94 . 6 107 . 3 ] ng / ml in Navsari Gujaratis ; F=76 . 6 , p < 0 . 001 ) . ^^^ These differences were still apparent when adjustment was made for BMI by location for IGF 1 ( F=57 . 4 , p < 0 . 001 ) and IGFBP 3 ( F=5 . 7 , p=0 . 02 ) , but were no longer apparent for IGFBP 1 and insulin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined myocardial expression of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGF receptor 1 ( IGF 1R ) and IGF 2R in fetal sheep with maternal undernutrition . ^^^ At necropsy on day 78 or day 135 of gestation , gravid uteri were recovered . mRNA expression of IGF 1 and IGF 2 in ventricles were measured with RT PCR , and protein expression of IGF 1R , IGF 2R , IGFBP 3 was quantitated with Western blot . ^^^ No difference was found in IGF 1 , IGF 2 or IGFBP 3 levels between the NR and C groups at either gestational age . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We compared their follicular fluid concentration of IGF 1 , IGFBP 1 and IGFBP 3 between the patients who became pregnant ( n = 11 ) versus those nonpregnant ( n = 42 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At 12 h , there was a decrease in the levels of both IGF 1 protein and mRNA and within 3 6 h there was an increase in the levels of both IGFBP 3 protein and mRNA . ^^^ When SW 837 cells were treated with EGCG for a longer time , i . e . , 96 h , a very low concentration ( 1 . 0 microg / ml ) of EGCG also caused inhibition of activation of IGF 1R , a decrease in the IGF 1 protein , and an increase in the IGFBP 3 protein . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There were no differences in plasma concentrations of total IGF 2 , free IGF 1 and 2 , IGFBP 3 , GHBP and ghrelin , whereas a small difference was found for IGFBP 2 ( 302+ / 97 vs . 263+ / 66 microg / L ; LNP vs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGFBP 3 mRNA expression showed a slight increase after treatment with 1 and 10 U / ml . alpha IFN ( 1 10 U / ml ) reduced the stimulatory effect of IGF 1 on cell replication ( p < 0 . 01 ) , inhibited ( p < 0 . 01 ) cell replication in untreated and in fetal calf serum ( FCS ) stimulated cells , and increased apoptosis in Calu 6 cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Peak GH after GHRH+arginine ( ARG ) test , serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) , lipid profile , homeostasis model assessment ( HOMA ) index and intima media thickness ( IMT ) at common carotid arteries were measured in 174 healthy individuals ( 92 women , 82 men , aged 18 80 yr ) . ^^^ An inverse correlation was found between the IGF 1 z score and total / HDL cholesterol ratio ( p = 0 . 02 ) and mean IMT ( p = 0 . 0009 ) ; IGFBP 3 z score and mean IMT ( p = 0 . 043 ) ; IGF : IGFBP 3 molar ratio and total / HDL cholesterol ratio ( p < 0 . 0001 ) and mean IMT ( p < 0 . 0001 ) . ^^^ At multi step regression analysis , age was the best predictor of HDL cholesterol levels and mean IMT , IGF 1 level was the best predictor of total cholesterol and total / HDL cholesterol ratio , the IGF I / IGFBP 3 molar ratio was the best predictor of triglycerides levels . ^^^ The z scores of IGF 1 and IGFBP 3 were the second best predictors of mean IMT after age . ^^^ In conclusion , IGF 1 and IGFBP 3 were negatively correlated with common cardiovascular risk factors , studied as total / HDL cholesterol ratio , and / or early atherosclerosis , studied as IMT at common carotid arteries . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Serum calcium , phosphate , alkaline phosphatase , parathormone ( PTH ) , vitamin D 3 , serum C telopeptides , IGFBP 3 and IGF 1 were measured at baseline , 6 , 12 , 18 and 24 months after surgery . ^^^ Serum telopeptides increased by 100 % ( P < 0 . 001 ) and serum IGFBP 3 decreased by 16 % ( P < 0 . 001 ) during the first 6 months , and then stabilized , whereas IGF 1 remained stable over the 2 y . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 1 binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and myoglobin were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Isolation and validation of human prepubertal skeletal muscle cells : maturation and metabolic effects of IGF 1 , IGFBP 3 and TNFalpha . ^^^ We have developed a primary skeletal muscle cell culture model derived from normal prepubertal children to investigate the effects of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) and tumour necrosis factor alpha ( TNFalpha ) on growth , differentiation and metabolism . ^^^ IGF 1 promoted whilst TNFalpha inhibited myoblast proliferation , differentiation and IGFBP 3 secretion . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Subjects underwent single slice , computerized tomography scanning to measure VAT and serum fasting insulin , IGF 1 , and IGFBP 3 measurements . ^^^ IGF 1 ( P = . 02 ) , IGF I / IGFBP 3 ratio ( P = . 003 ) , and insulin ( P = . 02 ) were significantly increased in subjects with adenomas compared with controls . ^^^ In an unadjusted logistic regression analysis using sex specific quartile cut points , subjects in quartile 4 in comparison with quartile 1 of IGF 1 ( odds ratio [ OR ] = 1 . 7 ; [ 95 % CI : 1 . 0 2 . 9 ] , Ptrend = . 03 ) , IGF I / IGFBP 3 ratio ( OR = 1 . 9 [ 95 % CI : 1 . 1 3 . 3 ] , Ptrend = . 01 ) , and insulin ( OR = 2 . 1 [ 95 % CI : 1 . 2 3 . 6 ] , Ptrend = . 04 ) were at increased risk of adenoma . ^^^ When limiting the case group to advanced adenomas , the effect was more pronounced : IGF 1 ( OR = 2 . 8 [ 95 % CI : 1 . 3 6 . 2 ] , Ptrend = . 006 ) , IGF I / IGFBP 3 ratio ( OR = 2 . 3 , [ 95 % CI : 1 . 0 5 . 2 ] , Ptrend = . 04 ) , and insulin ( OR = 2 . 3 [ 95 % CI : 1 . 1 4 . 9 ] , Ptrend = . 14 ) . ^^^ CONCLUSIONS : Levels of IGF 1 , ratio of IGF I / IGFBP 3 , and insulin are associated with adenomas and even more so with advanced adenomas . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : GH receptors ( GH R ) , IGF 1 , IGFBP 3 ( binding protein 3 ) , IGF 1 R and receptor substrates ( IRS ) were evaluated in cholangiocytes of normal or bile duct ligated ( BDL ) rat livers . ^^^ IGFBP 3 , which may counter regulate IGF 1 effects , was decreased in BDL cholangiocytes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH caused a significant rise in IGF 1 levels ( 290+ / 67 and 454+ / 71 microg / l for 1 / 2 PN+GH and Full PN+GH , respectively ) and restored serum IGFBP 3 and the acid labile subunit to normal , by the postoperative day 9 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Correlations between markers of inflammation and IGF 1 , IGF 2 and IGFBP 3 were examined in CD and UC patients in remission and relapse . ^^^ RESULTS : In the UC group we found correlations between IGF 1 and CRP ( r ( s ) = Spearman ' s rho ) ( r ( s ) = 0 . 40 , p < 0 . 01 ) and albumin ( r ( s ) = 0 . 46 , p < 0 . 001 ) , IGFBP 3 and albumin ( r ( s ) = 0 . 36 , p < 0 . 01 ) and AI score ( r ( s ) = 0 . 31 , p < 0 . 05 ) . ^^^ In the CD group we found correlations between IGF 1 and CRP ( r ( s ) = 0 . 40 , p < 0 . 05 ) , and albumin ( r ( s ) = 0 . 46 , p < 0 . 01 ) , IGFBP 3 and albumin ( r = 0 . 36 , p < 0 . 01 ) . ^^^ CONCLUSIONS : IGF 1 , IGF 2 and IGFBP 3 are correlated to albumin and IGF 1 and IGF 2 are correlated to CRP in IBD patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONTEXT : Poor sensitivity of IGF binding protein ( IGFBP ) 3 assessment in the work up of GH deficiency ( GHD ) has been ascribed to the equal affinity of IGFBP 3 for IGF 1 and IGF 2 and to IGFBP 3 proteolysis . ^^^ INTERVENTIONS : IGF 1 , IGF 2 , and IGFBP 3 serum concentrations were measured by immunoradiometric assay . ^^^ MAIN OUTCOME MEASURES : Main outcome measures were sensitivity and specificity of IGF 1 , IGF 2 , and IGFBP 3 measurements . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
He showed recurrent panniculitis since early infancy , and short stature with delayed skeletal age ( 3 years less ) and low levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This different growth rate correlated with growth hormone ( GH ) / insulin growth factor ( IGF ) axis dynamics : the donor had significantly lower insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) values than the recipient on postoperative days ( POD ) 3 30 , although they had similar GH values . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The proband has minor dysmorphic features , lordosis , lack of menarche , late signs of puberty , low prepuberal levels of gonadotrophins and steroids , but borderline low IGF 1 and normal IGF Bp 3 serum levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In liver cirrhosis the IGF axis is severely disturbed , and these alterations are associated with reduced IGF 1 , IGFBP 3 but elevated IGFBP 1 serum levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : The purpose of this study was to determine the age specific reference ranges for some important male sex steroid hormones , prostate specific antigen ( PSA ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) , for the Kuwaiti population . ^^^ The serum concentrations of total testosterone ( TT ) , dehydro epiandrosterone sulfate ( DHEAS ) , androstenedione ( ADT ) , sex hormone binding globulin , luteinizing hormone ( LH ) , follicle stimulating hormone ( FSH ) , prolactin , PSA , IGF 1 and IGFBP 3 were determined . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIMS / HYPOTHESIS : To find out whether the levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 1 ( IGFBP 1 ) , highly phosphorylated IGFBP 1 ( hpIGFBP 1 ) , and IGF binding protein 3 ( IGFBP 3 ) are related to the progression of diabetic retinopathy ( DR ) during pregnancy and postpartum . ^^^ Levels of serum total IGF 1 and two different phosphoisoform patterns of IGFBP 1 and IGFBP 3 were measured during the first and third trimester of pregnancy and 3 months postpartum . ^^^ RESULTS : Both the levels of serum total IGF 1 ( P < . 0001 ) and IGFBP 3 ( P= . 003 ) were lower in the diabetic than in the nondiabetic women during pregnancy and postpartum ( repeated measures ANOVA between the groups ) . ^^^ Additionally , the IGF 1 and IGFBP 3 levels tended to be lower in the diabetic women with more severe DR at baseline than in those with less severe DR . ^^^ There were no statistically significant differences in the levels of IGF 1 and IGFBP 3 in the diabetic women with progression of DR compared with those without . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 levels during initial GH dosage step up are indicators of GH sensitivity in GH deficient children and short children born small for gestational age . ^^^ We investigated GH sensitivity by studying IGF 1 and IGFBP 3 concentrations during the initial phase of GH treatment . ^^^ IGF 1 and IGFBP 3 were measured by means of published in house RIAs and age related references were used to calculate standard deviation scores ( SDS ) . ^^^ GH stimulated ( microg / l ) ( 5 . 6 vs . 10 . 8 ; p < 0 . 01 ) ; IGF 1 SDS ( 3 . 5 vs . 1 . 8 ; p < 0 . 01 ) ; IGFBP 3 SDS ( 2 . 0 vs . 0 . 8 ; p < 0 . 01 ) . ^^^ IGF 1 and IGFBP 3 increased as follows in GHD and SGA during stepping up of the dosage ( ng / ml , GHD vs . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
These complexes are referred to as binary complexes consisting of IGF 1 or IGF 2 and one IGFBP , or as ternary complexes each consisting of either of IGF 1 or IGF 2 , IGFBP 3 or 5 , and an acid labile subunit known as ALS . ^^^ The plasma concentrations of IGF 1 and IGFBP 3 were markedly reduced both in ALS KO mice and in the ALS deficient patients . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aims of this study were : ( 1 ) to examine the effect of different therapeutic modalities ( transsphenoidal surgery , TSS ; radiotherapy , RT ; medical treatment with somatostatin analogues , SSA ) on the daily spontaneous GH secretory pattern ( day curve ) ; and ( 2 ) to determine the relationship between the characteristics of the GH secretory pattern and the circulating concentration of IGF 1 , acid labile subunit ( ALS ) and IGFBP 3 . ^^^ IGF 1 , IGFBP 3 and ALS were measured in basal conditions . ^^^ No significant differences were observed with regard to the mean GH , IGF 1 , ALS or IGFBP 3 among the different treatment groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 , the acid labile subunit ( ALS ) , N terminal propeptide of type 1 collagen ( PINP ) , C terminal telopeptide of type 1 collagen ( ICTP ) and N terminal propeptide of type 3 collagen ( PIIINP ) were measured in samples collected at baseline ( two samples ) , after 10 , 22 and 24 days of r HuEPO treatment and at the end of the 4 week wash out period . ^^^ There was no significant treatment effect of r HuEPO compared to baseline on IGF 1 , IGFBP 3 , ALS , PINP , ICTP or PIIINP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Plasma concentration of adiponectin , resistin , ghrelin , total IGF 1 , free IGF 1 and IGFBP 3 were determined in all subjects . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the response of the somatotrope axis ( insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) ) to intense exercise in relation to tiredness . ^^^ RESULTS : The main finding of our study is that we observed strong negative correlations between IGF 1 ( r = 0 . 652 ) and IGFBP 3 ( r = 0 . 824 ) levels and the overtraining state estimated using the French Society of Sport Medicine questionnaire . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although we suspected her of having acromegaly because of high growth hormone ( GH ) levels ( 6 . 9 or 8 . 5 ng / ml ) , blood levels of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) were within normal range and the circadian rhythm of her blood GH levels was normally maintained . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 levels were lower in the children born SGA , both term and premature , compared to the controls ( p < 0 . 05 ) and especially in those who had catch up after the age of 6 months ( p < 0 . 002 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : Participants in the bottom quartiles of IGF 1 and IGFBP 3 levels ( median concentrations , 72 and 2937 ng / ml , respectively ) were at increased risk of ischemic stroke , e . g . adjusted odds ratios ( ORs ) of 2 . 06 [ 95 % confidence interval ( CI ) , 1 . 05 4 . 03 ] and 2 . 29 ( 95 % CI , 1 . 17 4 . 49 ) , respectively , when compared with participants in the top quartiles ( median concentrations , 125 and 4835 ng / ml , respectively ) . ^^^ No substantial associations were seen for IGF 1 and IGF 2 when also adjusting for IGFBP 3 ; adjusting IGFBP 3 for IGF 1 and 2 had only a minor impact on the risk estimates . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGFs ) play an important role in cell proliferation and apoptosis , and recent epidemiologic studies have suggested that circulating IGF 1 and IGF binding protein 3 ( IGFBP 3 ) may be related to colorectal , breast , and prostate cancer risk . ^^^ We measured plasma levels of IGF 1 and IGFBP 3 in a sequential sample of 333 population based control subjects enrolled in the Seattle Colorectal Cancer Family Registry from August 1999 through December 2001 , who had provided a blood sample . ^^^ Total IGF 1 and IGFBP 3 were measured from plasma samples using ELISA assays . ^^^ Multivariate adjusted linear regression was used to assess the associations between specific personal and lifestyle factors with IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At discharge and 6 , 12 , 18 , and 24 months after burn , height , weight , body composition , resting energy expenditure , muscle strength , and serum human growth hormone , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 , insulin , cortisol , parathyroid hormone , tri iodothyronine uptake ( T 3 uptake ) , and free thyroxine index ( FTI ) were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To analyze the differences in the plasma levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and their ratio between black and white men while controlling for factors that could confound the relationship between IGF levels and race . ^^^ Greater levels of IGF 1 and lower levels of IGFBP 3 have been associated with an increased risk of prostate cancer in many studies . ^^^ RESULTS : Our results indicated that black men have lower levels of IGFBP 3 and IGF 1 and a greater IGF I / IGFBP 3 ratio than white men across all ages younger than 70 , with and without an adjustment for height . ^^^ We found racial differences in the effect of age and height on levels of IGF 1 , IGFBP 3 , and the molar ratio . ^^^ Age had an inverse correlation with IGF 1 and IGFBP 3 levels in whites , but no such relationship in blacks . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH and LH secretion burst amplitude ( approximately 50 % ) and overnight pulsatile secretion ( approximately 50 % ) , IGF binding protein 1 ( +67 % ) , and free fatty acids ( +33 % ) increased , whereas leptin ( 47 % ) , total ( 27 % ) and free IGF 1 ( 32 % ) , total ( 24 % ) and free testosterone ( 30 % ) , and IGF binding protein 3 ( 6 % ) decreased . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the study was to estimate relationships between serum concentration of insulin like growth factor 1 ( IGF 1 ) , its binding protein ( IGFBP 3 ) , estradiol and testosterone in girls during puberty , taking into consideration gonadotropins level and nutritional status . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was no correlation of plasma IGFBP 4 level with age , sex , immunophenotype , or ALL risk group , and there was no correlation of IGFBP 4 level with plasma IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Football training decreased significantly the exercise stimulated GH levels all along the football season but did not have any significant effect on IGF 1 levels or on basal IGFBP 3 levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A number of biochemical endpoints were assessed including urinary phytoestrogen metabolites , lipids , antioxidant status , DNA damage and insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 1 ( IGFBP 1 ) and 3 ( IGFBP 3 ) . ^^^ However , in Phase 2 , concentrations of IGF 1 and IGFBP 3 were increased by phytoestrogen supplementation [ IGF 1 , median ( IQ range ) , baseline 155 ( 123 , 258 ) , postweek 265 ( 228 , 360 ) ng / ml , P < . 05 ; IGFBP 3 , baseline 3725 ( 3631 , 4196 ) , postweek 4420 ( 4192 , 4935 ) ng / ml , P < . 05 ] . ^^^ Short term phytoestrogen supplementation increases urinary phytoestrogen excretion and increases IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the relationship between plasma insulin , glucose , IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , apoptosis , and colorectal adenomas in a case control study . ^^^ Fasting plasma insulin , IGF 1 , IGF 2 , and IGFBP 3 levels were assessed by ELISA . ^^^ Overall , there were no significant differences between mean circulating levels of glucose , IGF 1 , IGF 2 , and IGFBP 3 among cases and controls and no association between these variables and apoptosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We assayed estradiol , progesterone , prolactin , sex hormone binding globulin ( SHBG ) , insulin like growth factor 1 ( IGF 1 ) , and IGFBP 3 in 111 African American and 111 Asian American women , matched to 111 Caucasian women on age , day of luteal phase , and day , time , and fasting status at blood collection . ^^^ In multivariate models , compared with Caucasians , African Americans had 18 % higher levels of estradiol ( P < 0 . 01 ) , 17 % higher free estradiol ( P < 0 . 01 ) , 11 % lower SHBG ( P = 0 . 05 ) , 11 % higher IGF 1 ( P < 0 . 01 ) , 25 % higher free IGF 1 ( P < 0 . 01 ) , and 9 % lower IGFBP 3 ( P < 0 . 01 ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We assessed the association of prediagnostic plasma levels of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and subsequent occurrence of prostate cancer in a case control study nested in the SU . ^^^ Frozen baseline plasma samples were used to measure IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 . ^^^ After controlling for the intervention group in the trial and the other IGF axis variables , the odds ratios and 95 % confidence interval ( 95 % CI ) comparing the upper quartile to the baseline quartile were 1 . 83 ( 95 % CI , 0 . 85 3 . 95 ) , 1 . 05 ( 95 % CI , 0 . 35 3 . 18 ) , 0 . 79 ( 95 % CI , 0 . 39 1 . 58 ) , and 0 . 42 ( 95 % CI , 0 . 12 1 . 52 ) for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , respectively . ^^^ More suggestive associations for IGF 1 and IGFBP 3 were observed with advanced and aggressive cancers . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Five serum samples at baseline , 3 , 6 , 12 , and 24 months were collected in the morning during the luteal phase and analysed for IGF 1 and IGFBP 3 by double antibody ELISA . ^^^ Randomisation resulted in similar mean IGF 1 and IGFBP 3 levels by group . ^^^ We did not observe a significant intervention effect on IGF 1 , IGFBP 3 , and their molar ratio during the entire study period . ^^^ However , urinary isoflavone excretion during the study period was positively associated with IGF 1 ( P=0 . 04 ) and the IGF 1 : IGFBP 3 ratio ( P=0 . 06 ) . ^^^ Adding soya foods to the diet of premenopausal women does not appear to lower serum levels of IGF 1 and IGFBP 3 ; if anything , the greater protein intake from soya may lead to a small increase in IGF 1 serum levels . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were measured using immunoradiometric assays . ^^^ Multiple linear regression analysis revealed that the only significant independent predictors of IGF 1 were IGFBP 3 and African admixture ( p < 0 . 01 for both ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONTEXT : Administration of IGF binding protein 3 ( IGFBP 3 ) with IGF 1 stabilizes IGF 1 concentrations and prolongs its half life . ^^^ Administration of IGF 1 and nonglycosylated IGFBP 3 for 2 wk to 32 subjects increased glycosylated IGF I / IGFBP 3 by 20 40 % . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Total ghrelin in CRF correlated positively with active ghrelin ( p < 0 . 001 ) , GH ( p < 0 . 05 ) , IGF 1 ( p < 0 . 05 ) , free IGF 1 ( p = 0 . 0001 ) , IGFBP 3 ( p < 0 . 01 ) , IGFBP 2 and 6 ( p < 0 . 05 ) . ^^^ Active ghrelin in CRF correlated positively with IGF 1 ( p < 0 . 001 ) , free IGF 1 ( p < 0 . 005 ) , IGFBP 2 ( p < 0 . 05 ) and IGFBP 3 ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured glucose , insulin , IGF 1 , IGFBP 1 , IGFBP 3 , glucagon , growth hormone ( GH ) , prolactin , adrenocorticotropin ( ACTH ) , cortisol and IL 6 in umbilical cord blood of infants born at term gestation after : A ) elective Cesarean section ( n = 37 ) , B ) normal delivery ( n = 87 ) or C ) complicated delivery ( n = 25 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In this study , IGF 1 , IGFBP 3 , IGFBP 1 and leptin levels were investigated in 27 children with PA aged 5 . 4 8 . 6 years and 13 healthy children aged 5 . 7 8 . 58 years . ^^^ IGF 1 ( p < 0 . 005 ) , IGFBP 3 ( p < 0 . 05 ) and leptin ( p < 0 . 0001 ) levels of lean PA girls were higher than controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
EGF mediated regulation of IGFBP 3 determines esophageal epithelial cellular response to IGF 1 . ^^^ Secreted IGFBP 3 neutralized IGFs and prevented IGF 1 receptor ( IGF IR ) activation . ^^^ In contrast , EGF suppressed IGFBP 3 mRNA and protein expression through activation of MAPK in an EGFR tyrosine kinase dependent manner to restore the cellular response to IGF 1 . ^^^ When stably overexpressed , wild type IGFBP 3 but not I56G / L80G / L81G ( GGG ) mutant IGFBP 3 , which has a reduced affinity to IGFs , prevented IGF 1 from activating IGF IR and Akt as well as stimulating cell proliferation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Free IGF 1 concentrations were negatively correlated ( P < 0 . 05 ) with insulin like growth factor binding protein ( IGFBP ) 2 , 4 and 5 but not IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MMP 19 promotes proliferation of keratinocytes by cleaving the insulin like growth factor binding protein 3 , thereby causing the release of IGF 1 , which is a potent growth factor for these cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We genotyped 22 common single nucleotide polymorphisms in 10 genes involved in GH production and action ( GHRH , GHRHR , SST , SSTR 1 SSTR5 , POU1F1 , and GH 1 ) , and in parallel , we measured serum levels of IGF 1 and IGFBP 3 , its major binding protein , in samples of cases and controls . ^^^ SSTR 5 polymorphisms were associated with IGF 1 levels , whereas one polymorphism in GHRHR and one in POU1F1 were associated with IGFBP 3 levels . ^^^ Our conclusion is that common genetic variation in the GH synthesis pathway , as measured by single nucleotide polymorphisms selected in the present study , is not a major determinant of IGF 1 and IGFBP 3 circulating levels , and it does not play a major role in altering breast cancer risk . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Genetic , anthropometric , and lifestyle factors associated with IGF 1 and IGFBP 3 levels in Hispanic and non Hispanic white women . ^^^ Circulating concentrations of IGF 1 and IGFBP 3 are associated with risk of pre menopausal breast cancer . ^^^ Racial differences in levels of these factors have been reported , and determinants of IGF 1 and IGFBP 3 levels within racial and ethnic groups are unclear . ^^^ In this study we examine genetic , anthropometric , diet , and lifestyle factors that may predict serum levels of IGF 1 and IGFBP 3 among Hispanic and non Hispanic white women . ^^^ Body size indicators , vigorous physical activity , and dietary factors appeared to influence serum levels of IGF 1 and the ratio of IGF 1 to IGFBP 3 in pre menopausal women more than in post menopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We have previously shown that exogenous recombinant porcine IGFBP 3 ( rpIGFBP 3 ) suppresses proliferation and differentiation of L 6 myogenic cells in an IGF 1 dependent manner and suppresses proliferation of L 6 myogenic cells via an IGF 1 independent mechanism . ^^^ Immunoneutralization of IGFBP 3 increased both IGF 1 and Long R 3 IGF 1 stimulated proliferation of tL 6 cells ( 58 and 33 % , respectively ) ( P < 0 . 01 ) . ^^^ Immunoneutralization of IGFBP 3 also increased IGF 1 stimulated differentiation ( 21 % , P < 0 . 05 ) but had no effect on Long R 3 IGF 1 stimulated differentiation of tL 6 myogenic cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Fasting IGF 1 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 , insulin and glucose ( 0 and 2 hour ) were measured . ^^^ RESULTS : Total energy and total fat intake were higher in UK migrants , as were IGFBP 3 and IGF 1 ( mean ( 95 % confidence interval ) : 145 . 9 ( 138 . 1 153 . 6 ) vs . 100 . 9 ( 94 . 6 107 . 3 ) ng ml ( 1 ) ; F=76 . 6 , P < 0 . 001 ) . ^^^ At both sites , IGF 1 correlated positively with total energy ( Spearman ' s rho=0 . 45 , P < 0 . 001 ) and total fat ( rho=0 . 44 , P < 0 . 001 ) as did IGFBP 3 with total energy ( rho=0 . 21 , P < 0 . 05 ) and fat ( rho=0 . 26 , P < 0 . 001 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : We studied the action of leptin ( 0 , 1 , 10 , or 100 ng / ml ) and immunoneutralization of IGF 1 using specific antiserum ( 0 . 1 % ) on the release of progesterone ( P ) , estradiol ( E ) , oxytocin ( OT ) , IGF 1 , IGFBP 3 , and prostaglandins F ( PGF ) by these cells using radioimmunoassay / immunoradiometric assay . ^^^ The addition of antiserum against IGF 1 decreased IGF 1 output , increased P , OT , IGFBP 3 , and PGF secretion , and had no effect on E release . ^^^ Immunoneutralization of IGF 1 also prevented or reversed the effects of leptin on P , E , IGF 1 , IGFBP 3 , PGF , but not on OT . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The production of the most abundant IGF 1 binding proteins ( IGFBPs ) in bone tissue , IGFBP 3 , 4 , and 5 , was lower in OA compared to normal Ob independently of the OA group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We studied expression of the ligands IGF 1 and IGF 2 , the inhibitory IGF binding protein 3 , the type 1 IGF receptor ( IGF IR ) , and the downstream mediator insulin receptor substrate 1 by immunohistochemistry in 56 tissue specimens ( 28 low grade and 28 high grade prostate adenocarcinomas ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The association of low IGF 1 and ALS with normal IGFBP 3 levels could explain growth impairment of M+ children and could suggest a GH resistance by a late postreceptor signaling defect . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To investigate the association of circulating levels of IGF 1 and its principle binding protein , IGF binding protein 3 ( IGFBP 3 ) , in childhood with subsequent measures of IQ . ^^^ METHODS : The cohort study was based on data for 547 white singleton boys and girls , members of the Avon Longitudinal Study of Parents and Children , with IGF 1 and IGFBP 3 measurements ( obtained at a mean age of 8 . 0 years ) and IQ measured with the Wechsler Intelligence Scale for Children ( at a mean age of 8 . 7 years ) . ^^^ For some children ( n = 407 ) , IGF 1 ( but not IGFBP 3 ) levels had been measured at approximately 5 years of age in a previous study . ^^^ Additionally controlling for maternal education and IGFBP 3 levels attenuated the associations ( change in IQ for every 100 ng / mL increase in IGF 1 levels : 2 . 51 points ; 95 % CI : 0 . 42 to 5 . 44 points ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study examines the serum concentrations of DHEA sulfate ( DHEAS ) , IGF 1 and IGF 1 binding protein 3 ( IGFBP 3 ) in 20 patients with CFS and in 12 normal controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Changes in total IGF 1 ( tIGF 1 ) and total IGF 2 ( tIGF 2 ) , free IGF 1 ( fIGF 1 ) , IGF binding proteins ( IGFBP 1 and IGFBP 3 ) , IGFBP 3 protease activity and insulin levels were examined in 31 patients with active CD ( CDAI : 186 603 ) during treatment with prednisolone ( 40 mg for 1 week ) and tapering the dose by 5 mg / week . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 correlated directly to lag phase ( r = 0 . 48 ; P = 0 . 01 ; r = 0 . 63 , P = 0 . 002 , respectively ) and to vitamin E ( r = 0 . 59 , P = 0 . 003 ; r = 0 . 58 , P = 0 . 006 , respectively ) . ^^^ By contrast , IGF 1 and IGFBP 3 correlated indirectly to MDA ( r = 0 . 47 , P = 0 . 01 ; r = 0 . 65 , P = 0 . 002 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : IGF 1 and IGFBP 3 decreased from +6 . 9 and 4 . 6 standard deviation scores ( SDS ) , respectively , to within normal range . ^^^ CONCLUSION : Pegvisomant normalized IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Similarly , the SNPs were tested for associations with serum growth hormone , IGF 1 , IGFBP 3 , prolactin , and anthropometric variables . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 and breast cancer in young women : a pooled re analysis of three prospective studies . ^^^ We pooled 220 cases of breast cancer diagnosed before age 50 , and 434 control subjects , from three prospective studies in New York ( USA ) , Ume ( Northern Sweden ) and Milan ( Italy ) , and we measured IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) with common enzyme linked immunosorbent assays . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) were measured by RIA . ^^^ RESULTS : No significant difference in height , spontaneous height velocity , IGF 1 , and IGFBP 3 levels was found at the start of rhGH therapy in the three GHR genotype groups studied . ^^^ At the first year of treatment , girls with Turner syndrome carrying one or two d 3 GHR alleles showed a significantly higher increment in height velocity ( P = 0 . 019 ) and exceeded their growth prediction significantly ( P = 0 . 007 ) , whereas their increments of IGF 1 and IGFBP 3 , weight , and height were not significantly different . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present paper was to evaluate the expression of growth factor IGF 1 , and growth factor binding proteins IGFBP 3 and IGFBP 5 genes and induction of NOS family members ( nNOS , eNOS and iNOS ) and TNF alpha genes together with glia activation , in the SN at 5 and 48 h after severe hypoxia in the 7 day old rat , a model for the term human fetus . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Baseline assessments of physical activity and body size were made , and cases with available plasma had pre diagnosis insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels measured . ^^^ RESULTS : A total of 526 cases of colorectal cancer were identified , of which 443 had IGF 1 / IGFBP 3 levels measured . ^^^ For the physically inactive , neither IGF 1 nor IGFBP 3 was associated with disease specific survival . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 tagging polymorphisms are associated with circulating levels of IGF 1 , IGFBP 3 and risk of breast cancer . ^^^ Experimental and observational studies in humans and animals suggest that insulin like growth factor 1 ( IGF 1 ) and its principal binding protein , IGFBP 3 , may influence breast cancer susceptibility . ^^^ We have examined the association of nine and four single nucleotide polymorphisms ( SNPs ) in the IGF 1 gene and in the IGFBP 3 genes , respectively , with circulating levels of their gene products in a population based study of 600 middle aged men and women , and in a breast cancer case control study , comprised 4647 cases and 4564 controls . ^^^ Our data indicate that common variants in the IGF 1 and IGFBP 3 genes are associated with differences in circulating levels of IGF 1 and IGFBP 3 and with breast cancer risk . ^^^ More specifically and consistent with experimental models , our data suggest that higher IGF 1 levels may increase the risk of breast cancer but higher IGFBP 3 levels may be protective . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
SGA children have elevated IGFI levels , possibly due to either hyperinsulinism or partial IGF 1 resistance , whereas VLBW children have low IGF 1 and IGFBP 3 levels suggestive of GH resistance . ^^^ IVF children are taller with increased IGF 1 , IGF 2 and IGFBP 3 expression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Although local expression of IGF 1 in the colon is increasingly recognised as having important regulatory consequences , the role of locally expressed IGFBP 3 remains unknown . ^^^ The aims of the present study were : ( 1 ) to quantify and localise the expression of IGFBP 3 in human normal and malignant colon ; ( 2 ) to relate this expression to that of other components of the IGF 1 axis ; and ( 3 ) to investigate the effects of IGFBP 3 on colonic epithelial cell proliferation and apoptosis . ^^^ IGFBP 3 , IGF 1 , IGF 1 receptor and GH receptor mRNA levels were quantified using real time RT PCR . ^^^ Human colorectal cancer HT 29 and CaCo 2 cells were cultured with IGFBP 3 ( 200 ng / ml ) , + / IGF 1 ( 20 ng / ml ) , + / sodium butyrate ( 5 mM ) . ^^^ IGF 1 mRNA was detected in 31 samples of normal colon ; mean IGFBP 3 copy number was higher in the IGF 1 positive samples compared with IGF 1 negative samples . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
By binding to its receptor , GH 1 stimulates the production of IGF 1 and its binding protein IGFBP 3 , resulting in the regulation of cell proliferation , differentiation and apoptosis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In a rat osteoblast cell culture that we studied , IGF 2 and IGFBP 2 were the most abundant peptides of the IGF system released in cell medium , IGFBP 5 was abundantly expressed but bound to cell surface and cell matrix and IGF 1 and IGFBP 3 were found at very low concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We also aimed to observe the effects of both Cd and Zn on the plasma levels of growth hormone ( GH ) , insulin like growth factor 1 ( IGF 1 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) in this study . ^^^ At the end of this period , plasma GH , IGF 1 , and IGFBP 3 of the animals were analyzed in the blood obtained . ^^^ According to our results , levels of IGF 1 and IGFBP 3 in the Cd administered group were significantly lower than those of controls ( p < 0 . 05 and p < 0 . 01 respectively ) . ^^^ These results show that although the addition of Zn to the diet of healthy rats had no effect on the levels of GH , IGF 1 , and IGFBP 3 , Cd addition lowered the levels of IGF 1 and IGFBP 3 but did not change the levels of GH compared to controls . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of the study was to determine whether the serum concentration of insulin like growth factor 1 ( IGF 1 ) and its binding protein 3 ( IGFBP 3 ) correlates with the mineral metabolism markers in children with idiopathic decrease in bone mass . ^^^ Serum concentration of IGF 1 was determined with radioimmunoassay and IGFBP 3 using immunoradiometry . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We investigated whether circulating insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels are associated with the risk of fibrocystic breast conditions ( FBC ) , in a case control study nested within a randomized trial of breast self examination conducted in Shanghai , China . ^^^ Circulating IGF 1 and IGFBP 3 levels and their molar ratio were positively associated with risk of FBC . ^^^ The odds ratios ( ORs ) and 95 % confidence intervals ( CI ) for the upper fourth of the distribution compared to the lowest fourth for IGF 1 , IGFBP 3 and their molar ratio were 3 . 02 ( 2 . 02 4 . 52 ) , 1 . 92 ( 1 . 37 2 . 71 ) and 2 . 26 ( 1 . 52 3 . 36 ) , respectively . ^^^ The strength of the association between IGF 1 levels and FBC was attenuated after adjustment for IGFBP 3 and that for IGFBP 3 was largely eliminated after adjustment for IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) is a main molecule that binds IGF 1 . ^^^ GH , IGF 1 and IGFBP 3 have important roles in growth and development . ^^^ In this study , we investigated the effects of exercise during pregnancy on maternal plasma levels of GH , IGF 1 and IGFBP 3 and on fetal development . ^^^ Blood samples were collected from pregnant rats on 0 , 7th , 14th and 20th days of gestation ( D ) under anesthesia with intracardiac puncture , and maternal plasma levels of GH , IGF 1 and IGFBP 3 were determined . ^^^ Plasma GH and IGF 1 levels were elevated in exercising pregnant rats on D 14 and D 20 , respectively , when compared to controls , and IGFBP 3 levels were increased on D 14 and D 20 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Using a quantitative sandwich immunoassay technique ( ELISA ) ( Quantikine , Human IGF 1 and IGFBP 3 , R & D Systems ) , we measured the concentration of IGF 1 and its major binding protein IGF binding protein 3 ( IGFBP 3 ) in serum drawn at the time of diagnosis from 77 Binet stage A CLL patients . ^^^ RESULTS : Either IGF 1 or IGFBP 3 were significantly decreased compared with healthy age and sex matched controls ( P < 0 . 0001 for both ; Mann Whitney test ) . ^^^ Serum levels of IGF 1 and IGFBP 3 paralleled each other ( P = 0 . 002 ) ; in contrast , no significant correlation was found between serum levels of IGF 1 and clinico hematological variables including age ( P = 0 . 253 ) , sex ( P = 0 . 270 ) , Rai clinical substages ( P = 0 . 140 ) , lactate dehydrogenase ( P = 0 . 956 ) , beta 2 microglobulin ( P = 0 . 368 ) , lymphocyte count ( P = 0 . 703 ) and lymphocyte doubling time ( LDT , P = 0 . 233 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We examined the associations of nutritional factors with IGF 1 and IGF binding protein 3 ( IGFBP 3 ) . ^^^ IGF 1 , IGFBP 3 , and nutritional factors in young black and white men : the CARDIA Male Hormone Study . ^^^ Serum IGF 1 and IGFBP 3 levels and dietary intake were measured in 459 black and 682 white male subjects of the Coronary Artery Risk Development in Young Adults study at the Year 7 ( 1992 1993 ) exam . ^^^ In black men , IGFBP 3 was positively associated with magnesium ( P = 0 . 02 ) , and one serving of milk per day was associated with an 8 . 23 ng / ml higher IGF 1 concentration ( P = 0 . 05 ) . ^^^ Tests for interaction , however , revealed no differences between blacks and whites in the associations of nutrients with IGF 1 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of leptin , sOB R , IGF 1 , and IGF binding protein 3 as well as anthropometric measurements were determined at the beginning of the study and at 2 wk intervals . ^^^ There were significant correlations between leptin and IGF 1 after 2 wk and IGF binding protein 3 during the whole study . sOB R was not correlated with any anthropometric data , whereas IGF 1 was a predictor of sOB R variance in the PEM toddlers ( 19 . 9 % , P = 0 . 022 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate serum Zn , IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels and to emphasize the potential beneficial effects of Zn supplementation for the prevention of growth failure in children with type 1 DM ( DM 1 ) . ^^^ Zn levels were measured by flame atomic absorption spectrophotometry ; IGF 1 and IGFBP 3 levels were measured by immunoradiometric assay . ^^^ Mean serum IGF 1 and IGFBP 3 levels were significantly lower in both prepubertal and pubertal diabetic groups compared to those of control groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In addition , we evaluated the effect of food intake and GH treatment on body composition and serum levels of IGF 1 , IGFBP 3 and leptin . ^^^ They also had significantly lower serum IGF 1 , IGFBP 3 and leptin levels . ^^^ GH treatment resulted in a significant increase of height , LBM , BMI , IGF 1 and IGFBP 3 SDS and a significant decrease of SF SDS and leptin SDS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Relationship between insulin like growth factors ( IGF 1 and IGF 2 ) , IGF binding proteins ( IGFBP 3 , IGFBP 2 ) , leptin and anthropometric parameters ( height , body mass index ) during antileukaemic treatment in children . ^^^ PURPOSE : The aim of the study was to estimate the anthropometric parameters and their relationship to serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin before and during intensive antineoplastic treatment for acute lymphoblastic leukaemia in children . ^^^ MATERIAL AND METHODS : In 46 children in median age 6 . 6 years ( range from 1 . 6 to 16 ) we evaluated at the time of diagnosis , after protocol 1 and after intensive treatment , height , body mass index ( BMI ) and IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin . ^^^ IGF 1 SDS was low and similar at each point , IGF 2 SDS and IGFBP 3 SDS values augmented progressively and IGFBP 2 SDS was significantly elevated before treatment and lowered ( but not normalized ) during the therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Several obesity related and physical activity related hormones and peptides may explain this association , including insulin , leptin , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 . ^^^ We observed lower C peptide ( P for trend = 0 . 001 ) and leptin ( P for trend = 0 . 001 ) levels and higher IGF 1 ( P for trend = 0 . 0037 ) and IGF binding protein 3 ( P for trend = 0 . 055 ) levels with higher levels of physical activity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Blood samples were collected at the time of mammography , and plasma IGF 1 and IGFBP 3 levels were measured by ELISA . ^^^ Adjusted mean breast density was assessed by joint levels of IGF 1 and IGFBP 3 using generalized linear models . ^^^ Among premenopausal women , high levels of IGF 1 and low levels of IGFBP 3 were independently correlated with high breast density ( r ( s ) = 0 . 083 ; P = 0 . 021 and r ( s ) = 0 . 124 ; P = 0 . 0005 , respectively ) . ^^^ Correlation of IGF 1 with breast density was stronger among women in the lowest tertile of IGFBP 3 than among those in the highest tertile of IGFBP 3 ( r ( s ) = 0 . 138 ; P = 0 . 027 and r ( s ) = 0 . 039 ; P = 0 . 530 , respectively ) . ^^^ In contrast , the correlation of IGFBP 3 with breast density was stronger among women in the highest tertile of IGF 1 than among those in the lowest tertile of IGF 1 ( r ( s ) = 0 . 150 ; P = 0 . 016 and r ( s ) = 0 . 008 ; P = 0 . 904 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DHT and testosterone , but not DHEA or E 2 , differentially modulate IGF 1 , IGFBP 2 , and IGFBP 3 in human prostatic stromal cells . ^^^ We investigated the actions of DHT , T , DHEA , and estradiol ( E 2 ) on insulin like growth factor ( IGF ) 1 , IGF 2 , IGF 1 receptor ( R ) , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 5 in primary cultures of human prostatic stromal cells by assessing cell proliferation , mRNA expression , and protein secretion by MTT growth assay , quantitative real time PCR , and ELISA , respectively . ^^^ DHT and T each increased IGF 1 ( 7 fold ) and decreased IGFBP 3 ( 2 fold ) mRNA expression and protein secretion in a dose and time dependent manner and increased IGFBP 2 ( 2 fold ) mRNA in a dose and time dependent manner . ^^^ The effects of DHT on IGF 1 , IGFBP 2 , and IGFBP 3 were more pronounced in stromal cultures that did not express desmin . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
However , most of the circulating IGF 1 associates with a high molecular weight complex approximately 150 KDa consisting of IGFBP 3 and the acid labile subunit ( ALS ) ADDIN . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : The serum concentrations of somatotrophin axis hormones ( growth hormone GH , insulin like growth factor 1 IGF 1 and insulin like growth factor binding protein 3 IGFBP 3 ) in the patients with psoriasis in its active stage have been evaluated in relation to the control group consisting of healthy people in order to see whether these hormones may play a role in the psoriasis aetiopathogenesis . ^^^ GH and IGFBP 3 concentrations in serum were determined by immunoradiometric assay ( IRMA ) , IGF 1 concentrations by radioimmunological assay ( RIA ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IL 1beta , IL 6 , TNFalpha , insulin and C peptide were measured . ^^^ Serum concentrations of IGF 1 and IGF 2 were significantly lower and insulin and C peptide levels significantly increased in CF compared with healthy controls whereas IGFBP 3 serum concentrations were similar , with comparable IGF I / IGFBP 3 and decreased IGF I / IGFBP 2 and IGF II / IGFBP 2 molar ratios . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Circulating GH , IGF 1 , IGFBP 3 , and sex steroid concentrations decrease with age . ^^^ Before treatment , in women and men , IGF 1 was directly related to IGFBP 3 ( P < 0 . 001 and P < 0 . 0001 ) and IGFBP 1 to IGFBP 2 ( P = 0 . 0001 ) . ^^^ Additionally , HRT attenuated or prevented GH mediated increases in IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Biochemical analyses of insulin , IGF 1 , IGFBP 1 , IGFBP 3 , IL 6 , high sensitivity ( hs ) CRP and other routine markers . ^^^ Both IGF 1 and IGFBP 3 , but not IGFBP 1 , were significantly correlated with age ( rho= 0 . 25 for IGF 1 and 0 . 35 for IGFBP 3 ; P < 0 . 001 ) and hsCRP ( rho= 0 . 21 and 0 . 32 ; P < 0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Laboratory data , including an undetectable TSH , low free T 4 , low IGF 1 and IGF binding protein 3 , prolactin deficiency , and LH and FSH deficiency were consistent with hypopituitarism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A prospective study of serum insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 in 942 healthy infants : associations with birth weight , gender , growth velocity , and breastfeeding . ^^^ MAIN OUTCOME MEASURES : Height , weight , and serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) were the main outcome measures . ^^^ IGF 1 and IGFBP 3 levels were positively associated with weight gain and height gain from birth to 3 months of age in AGA , but not in SGA , children . ^^^ SGA children had significantly lower IGF 1 [ 88 . 0 ng / ml ( 28 , 145 ) ; P = 0 . 05 ] and IGFBP 3 [ 1835 ng / ml ( 1180 , 2793 ) ; P < 0 . 001 ] levels than AGA children . ^^^ CONCLUSION : We found a significant , but weak , association between IGF 1 and IGFBP 3 levels at 3 months and postnatal growth in AGA , but not SGA , children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Because laboratory studies suggest that the actions of vitamin D , calcium , insulin like growth factor ( IGF ) 1 , and IGF binding protein 3 ( IGFBP 3 ) on human breast cancer cells are interrelated , we examined whether IGF 1 and IGFBP 3 levels could affect the strength of the association of vitamin D and calcium intakes with breast density . ^^^ Among 771 premenopausal women , breast density was measured by a computer assisted method , vitamin D and calcium intakes by a food frequency questionnaire , and levels of plasma IGF 1 and IGFBP 3 by ELISA methods . ^^^ This is the first study to report that the negative relation of vitamin D and calcium intakes with breast density may be seen primarily among women with high IGF 1 or high IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to investigate the roles of IGFBP 4 and 5 in age dependent bone loss and vertebral fracture risk in postmenopausal Japanese women and to compare them with those of IGF 1 and IGFBP 3 . ^^^ Serum levels of IGFBP 4 and 5 as well as IGF 1 and IGFBP 3 were measured by radioimmunoassay . ^^^ Serum levels of IGF 1 , IGFBP 3 , and IGFBP 5 declined with age , while serum IGFBP 4 increased with age . ^^^ Serum IGF 1 level was significantly and positively correlated with BMD at all sites except the femoral neck ( P < 0 . 01 ) , while serum IGFBP 3 and 4 levels were significantly and positively correlated with only radial BMD ( P < 0 . 01 ) . ^^^ Serum IGF 1 , IGFBP 3 , and IFGBP 5 levels were significantly lower in women with vertebral fractures than in those without fractures ( mean + / SD : 97 . 1 + / 32 . 1 vs . 143 . 9 + / 40 . 9 ng / dl , P < 0 . 0001 ; 2 . 18 + / 1 . 02 vs . 3 . 23 + / 1 . 07 microg / ml , P < 0 . 0001 ; 223 . 6 + / 63 . 3 vs . 246 . 5 + / 71 . 5 ng / ml , P = 0 . 0330 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONTEXT : GH insensitivity syndrome ( GHIS ) , Laron syndrome , is characterized by severe short stature , high serum GH levels , and very low serum IGF 1 and IGF binding protein 3 ( IGFBP 3 ) levels associated with a genetic defect of the GH receptor . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Polymorphisms of genes coding for insulin like growth factor 1 and its major binding proteins , circulating levels of IGF 1 and IGFBP 3 and breast cancer risk : results from the EPIC study . ^^^ Most of circulating IGF 1 is bound to an acid labile subunit and to one of six insulin like growth factor binding proteins ( IGFBPs ) , among which the most important are IGFBP 3 and IGFBP 1 . ^^^ We genotyped 23 common single nucleotide polymorphisms in IGF 1 , IGFBP 1 , IGFBP 3 and IGFALS , and measured serum levels of IGF 1 and IGFBP 3 in samples of cases and controls . ^^^ We found a weak but significant association of polymorphisms at the 5 ' end of the IGF 1 gene with breast cancer risk , particularly among women younger than 55 years , and a strong association of polymorphisms located in the 5 ' end of IGFBP 3 with circulating levels of IGFBP 3 , which confirms previous findings . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Compared with age range matched normal subjects , decreased IGFBP 3 , MMP 9 levels , unchanged IGF 1 were found in RA group whereas decreased IGF 1 levels , unchanged IGFBP 3 and increased MMP 9 at age > 30 years were found in RHD group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of CRP , serum amyloid A ( SAA ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNFalpha ) , antibodies against oxidized LDL , insulin and insulin like growth factor 1 ( IGF 1 ) along with insulin like growth factor binding protein 1 ( IGFBP 1 ) and IGFBP 3 were analyzed before and during the two treatments . ^^^ Both preparations lowered IGF 1 and raised IGFBP 1 and IGFBP 3 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Strength training leading to RF resulted in reductions in resting concentrations of IGF 1 and elevations in IGFBP 3 , whereas NRF resulted in reduced resting cortisol concentrations and an elevation in resting serum total testosterone concentration . ^^^ Elevation in IGFBP 3 after resistance training may have been compensatory to accommodate the reduction in IGF 1 to preserve IGF availability . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Plasma oestradiol , unconjugated oestriol , testosterone , progesterone , prolactin , sex hormone binding globulin ( SHBG ) , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) levels were measured in duplicates in the same batch ( batch 1 ) . ^^^ Intra class correlation coefficients ( ICC ) were higher than 0 . 9 for assay of SHBG [ 95 % CI 0 . 92 , 1 . 0 ] and progesterone [ 95 % CI 0 . 87 , 0 . 97 ] and between 0 . 8 and 0 . 9 for assay of oestradiol , unconjugated oestriol , prolactin , IGF 1 , and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum levels of total and free IGF 1 , IGFBP 3 , creatinine and haemoglobin A ( 1c ) ( HbA ( 1c ) ) , as well as urinary albumin excretion rate , creatinine clearance and blood pressure , were measured prior to the start of treatment and after 10 weeks of treatment . ^^^ RESULTS : At baseline , serum levels of IGFBP 3 were elevated and serum levels of free IGF 1 were reduced . ^^^ Losartan tended to reduce IGFBP 3 levels and to increase free IGF 1 levels , although neither effect was statistically significant . ^^^ These effects were more pronounced in a subanalysis of 18 losartan treated patients with stable metabolic parameters , with a decrease in IGFBP 3 from 133 . 2 to 122 . 6 nmol / l ( P=0 . 006 ) and an increase in free IGF 1 levels by 8 % ( ns ) . ^^^ Furthermore , changes in total IGF 1 and IGFBP 3 correlated to changes in serum creatinine levels in the metabolically stable patients ( r=0 . 58 , P=0 . 02 and r=0 . 6 , P=0 . 01 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Hence , our objective is to examine relationships between IGF 1 levels ; body mass index ( BMI ) ; fasting insulin level ; IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 levels ; and an array of traditional cardiovascular risk factors . ^^^ Further adjusting for BMI and IGFBP 3 level simultaneously strengthened the inverse association , with an odds ratio of 0 . 42 ( 95 % confidence interval , 0 . 22 0 . 80 ) for hypertension comparing the fourth with the first IGF 1 quartile . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Human corneal fibroblasts were incubated with TGF beta 2 or IGF 1 , to investigate IGF 1 , IGF 2 , IGFBP 3 , type 1 collagen , and alpha smooth muscle actin ( alpha SMA ) mRNA , as well as IGFBP 3 protein expression , during myofibroblast differentiation . ^^^ RESULTS : TGF beta 2 treatment induced expression of IGF 1 and IGFBP 3 mRNA and of IGFBP 3 protein in human corneal fibroblasts . ^^^ IGF 1 potently stimulated basal DNA synthesis , whereas IGFBP 3 inhibited it . ^^^ IGF 1 potently stimulated proliferation of TGF beta 2 activated myofibroblasts without reversing the activated fibrogenic phenotype , whereas IGFBP 3 suppressed IGF 1 induced proliferation of corneal fibroblasts . ^^^ CONCLUSIONS : These results suggest that during corneal wound healing , TGF beta stimulates IGF axis components , whereas IGFBP 3 may modulate IGF 1 induced myofibroblast proliferation to suppress corneal mesenchymal overgrowth . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND PARTICIPANTS : We analyzed data from 429 participants of the Boyd Orr cohort , for whom height measured in childhood ( mean age , 7 . 4 yr ) in 1937 1939 could be related to levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 in adulthood ( mean age , 71 . 1 yr ) . ^^^ Height in adulthood was not associated with IGF 1 , IGF 2 , or IGFBP 3 and was inversely associated with IGFBP 2 ( P = 0 . 05 ) after additionally controlling for childhood height . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF based investigations ( IGF 1 and IGF binding protein 3 ) are now replacing GH based investigations for evaluation and monitoring of disorders of the GH IGF axis . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In 27 children with AML ( mean age 13 . 6+ / 5 . 3 years ; patients in remission n=15 with relapse n=12 ) serum parameters of IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 were analyzed up to 18 months after HSCT by RIA . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We evaluated the effect of polymorphisms in four insulin related genes ( G972R in IRS 1 , G1057D in IRS 2 , a CA repeat in IGFI and an A / C polymorphism at 202 of IGFBP 3 ) on the risk of microsatellite instability and KRAS 2 and TP 53 mutations in a population based set of 1788 cases of colon cancer and 1981 controls . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIM : To determine whether recombinant human insulin like growth factor 1 ( rhIGF 1 ) administration to children with low IGF 1 and relatively low insulin like growth factor binding protein 3 ( IGFBP 3 ) serum concentrations would result in hypoglycemia . ^^^ METHODS : Eighteen children age 11 19 y with serum levels of IGF 1 < 2 SDS and IGFBP 3 < 0 SDS were randomly assigned to receive rhIGF 1 at 80 microg / kg body weight daily ( n = 6 ) , 40 microg / kg twice daily ( n = 6 ) , or 80 microg / kg twice daily ( n = 6 ) . ^^^ CONCLUSION : In normal individuals with low serum concentrations of IGF 1 and relatively low concentrations of IGFBP 3 , the administration of therapeutic doses of rhIGF 1 , while maintaining reasonable food intake , does not result in hypoglycemia . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Ghrelin , IGF 1 and IGFBP 3 levels in children with type 1 diabetes mellitus . ^^^ This aim of this study was to investigate ghrelin level , and its relationship with IGF 1 and IGFBP 3 levels in children with type 1 diabetes mellitus ( DM 1 ) . ^^^ Ghrelin levels were similar , and IGF 1 and IGFBP 3 levels were lower , in prepubertal and pubertal patients compared to controls . ^^^ In the patient group , ghrelin levels were negatively correlated with chronological age , height , weight , pubertal status and IGF 1 , but had no correlation with fasting glucose , HbA1c , insulin dose , duration of insulin therapy , and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGFBP 3 levels of Turkish children during childhood and adolescence : establishment of reference ranges with emphasis on puberty . ^^^ AIMS / METHODS : We established age and sex related reference ranges for serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels in 807 healthy Turkish children ( 428 boys , 379 girls ) , and constructed a model for calculation of standard deviation scores of IGF 1 and IGFBP 3 according to age , sex and pubertal stage . ^^^ RESULTS : Serum IGF 1 and IGFBP 3 concentrations tended to be higher in girls compared to boys of the same ages , but the differences were statistically significant only in pubertal ages ( 9 14 years ) for IGF 1 and only in prepubertal ages for IGFBP 3 ( 6 8 years ) ( p < 0 . 05 ) . ^^^ Serum levels of IGF 1 and IGFBP 3 increased steadily with age in the prepubertal stage followed by a rapid increase in IGF 1 in the early pubertal stages . ^^^ A relatively steeper increase in IGF 1 but not in IGFBP 3 levels was observed at age 10 11 years in girls and at 12 13 years in boys which preceded the reported age of pubertal growth spurt . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The objective of the study was to evaluate the changes in serum levels of GH during overnight profiles , IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) in short small for gestational age ( SGA ) children during GH treatment with two doses . ^^^ MAIN OUTCOME MEASURES : At baseline and after 6 months of GH treatment , overnight GH profiles were performed , and serum IGF 1 and IGFBP 3 levels were measured . ^^^ The IGF 1 and IGF 1 to IGFBP 3 ratio sd scores increased significantly in both groups , but were higher in group B than A [ 1 . 5 vs . 0 . 2 ( P = 0 . 002 ) and 1 . 4 vs . 0 . 3 ( P = 0 . 007 ) , respectively ] . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We measured ( 1 ) the lengths of both lower legs ( by knemometry ) at weeks 1 , 2 , 6 and 12 , ( 2 ) height and weight at presentation and at the second year follow up , and ( 3 ) levels of IGF 1 , IGFBP 3 , collagen markers and bone alkaline phosphatase at weeks 1 and 12 , and in year 2 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In the present study , circulating IGF 1 , IGF 2 and their binding proteins ( IGFBP 3 , IGFBP 2 and IGFBP 1 ) were determined in 92 adult patients with gastrointestinal inflammation ( Crohn ' s disease , colitis ulcerosa , gastritis , duodenitis errosiva , gastrointestinal candidiasis , and rotaviral and adenoviral enteritis ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding proteins 3 ( IGFBP 3 ) , a specific IGF 1 binding protein , is the major regulatory factor of IGF 1 activity . ^^^ METHODS : IGFBP 3 and IGF 1 secretion into gingival crevicular fluid ( GCF ) was analyzed by Western blotting and immunoradiometric assay ( IRMA ) , respectively , in GCF of 6 healthy subjects just prior to and during orthodontics treatment using fixed appliances . ^^^ Alterations in IGFBP 3 appear to be unrelated to the binding to IGF 1 , suggesting an IGF independent role of this binding protein in tooth movement . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
There was a transient increase in plasma IGF 1 and IGFBP 3 concentrations at fetal 90 d in prenatal T treated fetuses . ^^^ Hepatic IGF 1 mRNA and plasma IGFBP 3 content were reduced by 140 d when body weight was reduced in prenatal T treated fetuses . ^^^ Plasma IGFBP 2 content was significantly reduced in prenatal T treated newborns , but by 4 months these females had significantly higher circulating IGF 1 and IGFBP 3 concentrations and faster growth rates than control females . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Insulin related genes evaluated were the Bsm 1 , polyA , and Fok 1 polymorphisms of the VDR gene ; the G972R IRS 1 polymorphism ; the G1057D IRS 2 polymorphism ; the 19CA repeat polymorphism of the IGF 1 gene ; and the 200A > C IGFBP 3 polymorphism . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Interrelationships between serum leptin , IGF 1 , IGFBP 3 , C peptide and prolactin and breast cancer risk in young women . ^^^ We measured levels of several metabolic markers that are modified by obesity [ measured by body mass index ( BMI , ( weight ( kg ) / height ( m 2 ) ) ) ] and play a role in the reproductive axis , including , leptin , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , C peptide and prolactin in 233 cases and 251 controls participating in a retrospective study of breast cancer in young women conducted in the Seattle / Puget Sound region between 1990 and 1992 . ^^^ IGF 1 , IGFBP 3 , C peptide and prolactin were not related to breast cancer risk in a dose dependent manner . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Interestingly , plasma IGF 1 did not augment in rats with testicular atrophy treated with IGF 1 , while IGFBP 3 levels , that reduces IGF 1 availability , was increased in this group ( p < 0 . 05 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : We investigated a case of IGFD in which serum IGF 1 and IGFBP 3 were abnormally low , yet growth failure was modest ( 2 . 1 sd score at 15 . 5 yr of age ) . ^^^ The subject had a normal fasting GH level of 3 . 7 muU / ml and normal serum GH binding protein level ( 1258 pmol / liter ; normal range 431 1892 pmol / liter ) , but serum IGF 1 and IGFBP 3 were profoundly reduced ( 5 . 8 and 7 . 2 sd score , respectively , at age 12 . 3 yr ) , even through puberty . ^^^ CONCLUSIONS : ALS is critical for maintaining normal serum concentrations of IGF 1 and IGFBP 3 , most likely by prolonging the half lives of both proteins . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
LPS administration induced a significant decrease in body weight and in serum concentrations of IGF 1 and IGFBP 3 ( P < 0 . 01 ) , as well as in their gene expression in the liver . ^^^ Pretreatment of the animals with gadolinium chloride blocked the inhibitory effect of LPS on body weight , and on serum concentrations of IGF 1 , IGFBP 3 and nitrites , as well as growth hormone receptor ( GHR ) , IGF 1 and IGFBP 3 gene expression in the liver . ^^^ These results suggest that Kupffer cells are important mediators in the inhibitory effect of LPS on GHR , IGF 1 and IGFBP 3 gene expression in the liver , leading to a decrease in serum concentrations of IGF 1 and IGFBP 3 . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Specimen processing time and measurement of total insulin like growth factor 1 ( IGF 1 ) , free IGF 1 , and IGF binding protein 3 ( IGFBP 3 ) . ^^^ However , little is known regarding the effects of blood specimen processing time on measured levels of total and free IGF 1 , and on IGFBP 3 , the major binding protein . ^^^ Total IGF 1 , free IGF 1 , and IGFBP 3 levels were determined using commercial enzyme linked immunosorbent assays ( ELISAs ) commonly employed in epidemiologic studies . ^^^ RESULTS : Total IGF 1 and IGFBP 3 levels did not vary significantly with the amount of time at ambient temperature following centrifugation , even up to 24 h , in blood specimens that were centrifuged soon after collection ( all pchi 2 ) . ^^^ Total IGF I / IGFBP 3 molar ratio , a crude measure of free IGF 1 levels , showed no clear association . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
High levels of circulating insulin like growth factor 1 ( IGF 1 ) and its major binding protein ( IGFBP 3 ) at premenopausal ages have been associated with an increased breast cancer risk . ^^^ The mammographically dense area in the breast increased with increasing serum levels of IGF 1 ( P for linear trend , P ( t ) = 0 . 05 ) , IGF 2 ( P ( t ) = 0 . 08 ) , and IGFBP 3 ( P ( t ) = 0 . 01 ) only in premenopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIM : To analyze the changes in IGF 1 , IGFBP 3 , leptin and insulin after replacement doses of recombinant human growth hormone ( rhGH ) in short prepubertal children with chronic renal failure ( CRF ) . ^^^ Serum leptin , insulin , glucose , IGF 1 and IGFBP 3 were measured before , 6 and 12 months after beginning rhGH treatment . ^^^ There was a significant increment of IGF 1 and IGFBP 3 during the use of rhGH . ^^^ CONCLUSIONS : The replacement doses of rhGH during 12 months in a selected group of CRF children determined an increment in IGF 1 and IGFBP 3 , associated to normal serum leptin and insulin resistance . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to investigate whether moderately elevated IL 6 levels have short term effects on circulating IGF 1 , IGFBP 1 and IGFBP 3 proteolysis in vivo . ^^^ There was no effect of IL 6 on IGFBP 3 proteolysis , total IGF 1 or free dissociable IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Limited data is available concerning a possible association between early epithelial lining fluid ( ELF ) concentrations of IGF 1 ( total and free forms ) , IGF binding protein 3 ( IGFBP 3 ) , beta 2 microglobulin and subsequent development of CLD in preterm neonates . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Comparison of follicular fluid IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and PAPP A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF embryo transfer patients . ^^^ This is the first study that has compared follicular fluid ( FF ) IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and pregnancy associated plasma protein ( PAPP ) A concentrations , and their ratios , to investigate whether there was any difference in the intrafollicular microenvironment between the GnRH agonist ( GnRHa ) and antagonist ( GnRHant ) protocols for controlled ovarian stimulation ( COS ) . ^^^ IGF 1 , IGF 2 and IGFBP 3 concentrations were measured by radioimmunoassay and IGFBP 4 and PAPP A by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This pattern was associated with a favourable modulation of circulating IGF 1 and its main binding protein ( IGF binding protein 3 , IGFBP 3 ) , which have been associated with breast cancer risk in premenopausal women in different prospective studies . ^^^ In a subsequent biomarker study on premenopausal women who had participated in the phase 3 trial , high IGF 1 and low IGFBP 3 baseline levels were found to predict second breast cancer risk , although the magnitude of their changes during treatment did not fulfil the requirements for suitable surrogate end point biomarkers . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
As the measurement of IGF 1 and IGFBP 3 levels as well as the spontaneous GH secretion are not considered reliable parameters , the diagnosis of GHD in adults may be established by GH provocative tests , provided that a reproducible test with clear normative limits is available . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To investigate the IGF dependent and IGF independent effects of IGFBP 3 on prostate tumor growth , we crossed LPB Tag mice with cytomegalovirus ( CMVBP 3 ) and phosphoglycerate kinase ( PGKBP 3 ) mice that overexpress IGFBP 3 under the cytomegalovirus promoter and the phosphoglycerate kinase promoter , respectively , and also I56G / L80G / L81G mutant IGFBP 3 ( PGKmBP 3 ) mice that express I56G / L80G / L81G IGFBP 3 , a mutant , that does not bind IGF 1 but retains IGF independent proapoptotic effects in vitro . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In experiments with saline infusion , levels of ghrelin ( P < 0 . 001 ) , C peptide ( P < 0 . 001 ) , glucagon ( P < 0 . 001 ) , adiponectin ( P = 0 . 005 ) , cortisol ( P = 0 . 012 ) , IGF 1 ( P < 0 . 001 ) , IGFBP 3 ( P = 0 . 038 ) , and IGFBP 1 ( P = 0 . 001 ) fell in response to euglycemic hyperinsulinemia . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : The present study evaluated the role of activin A , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) in Egyptian patients suffering from combined hepatitis C virus ( HCV ) infection and hepatic schistosomiasis . ^^^ Furthermore , serum IGF 1 and IGFBP 3 levels were significantly reduced in patients with HCV , HCV + SHF and those with HCC compared to the control group . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum markers of bone metabolism were : OC , PICP , ICTP , 25 OH vit D and 1 , 25 ( OH ) ( 2 ) vit D , IGF 1 , IGFBP 3 and intact PTH . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
DESIGN AND METHODS : At baseline , circulating GH , IGF 1 , IGFBP 3 , leptin and lipid ( cholesterol and tri glycerides ) levels were measured in 33 acromegalic patients nine years after treatment with surgery of whom 6 were additionally irradiated . ^^^ Mean IGF 1 and IGFBP 3 levels were not different between GHD and GH sufficient cured acromegalics . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Taken together , these data provide direct evidence for combinatorial effects of IGFBP 3 , 4 , and 5 in both metabolism and at least some soft tissues and strongly suggest overlapping roles for IGFBP 3 and 5 in maintaining IGF 1 mediated postnatal growth in mice . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It is a strong antioxidant and modulates the insulin like growth factor ( IGF ) system by increasing circulating levels of IGF binding protein 3 ( IGFBP 3 ) and decreasing levels of IGF 1 . ^^^ Blood levels of IGFBP 3 , IGF 1 , and the oxidative DNA damage pyrimidopurinone adduct of deoxyguanosine ( M1dG ) were determined . ^^^ Intervention with silipide did not affect circulating levels of IGFBP 3 , IGF 1 , or M1dG . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
It shows that only in a few instances , such as for example , between IGF 1 and IGFBP 3 , and IGFBP 1 and leptin , significant and substantial genetic ( and environmental ) correlations were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We report on a 10 yr old Caucasian girl affected by Costello syndrome with fasting hypoglycemia and short stature , associated low circulating levels of acid labile subunit ( ALS ) , relatively low levels of IGF 1 and IGFBP 3 , and normal IGF 2 , mostly circulating in a binary complex with IGFBP 2 and 6 instead of in a 150 kDa ternary complex . ^^^ The reduced ALS concentration and the consequent impaired formation of the circulating 150 kDa ternary complex can induce an accelerated clearance rate of IGF peptides and of IGFBP 3 , contributing to the decreased IGF 1 growth promoting activity in our patient . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Especially human IGFBP 3 but also bovine IGFBP 2 added to cell cultures inhibited the rate of proliferation of C2C12 myoblasts stimulated by human IGF 1 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Anthropometry , IGF 1 and insulin like growth factor binding protein 3 ( IGFBP 3 ) were measured by standard methods . ^^^ After adjusting for energy , both cows ' milk and dairy product intakes were positively associated with IGF 1 ( P=0 . 040 and 0 . 027 , respectively ) and IGFBP 3 levels ( P=0 . 082 and 0 . 067 , respectively ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To further examine the utility of IGF F 1 1 as an IGF 1 antagonist we tested its ability to inhibit IGFBP 2 and IGFBP 3 binding to IGF 1 , ( 125 ) 1 IGF 1 binding to IGF 1Rs and to block IGF 1 induced Akt activation , cell cycle changes and [ ( 3 ) H ] thymidine incorporation in MCF 7 cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The aim of the present study was to evaluate the mediating role played by obesity on the relationship of free insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) with muscle strength and physical performance . ^^^ After adjusting for potential confounders , no significant association of IGF 1 and IGFBP 3 with study outcomes was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
At discharge , 6 , 12 , 18 , and 24 months after burn height , weight , body composition , resting energy expenditure ( REE ) , serum growth hormone , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , insulin , cortisol , parathyroid hormone , and thyroid hormones were measured . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
A subset of 28 patients ( 13 TBI+POLY , 15 POLY ) underwent serological testing for the following bone turnover parameters : carboxy terminal extension peptide of type 1 procollagen ( P1CP ) , pyridinolene cross linked carboxy terminal telopeptide ( 1CTP ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and basic fibroblast growth factor ( bFGF ) . ^^^ IGFBP 3 , an IGF 1 inhibiting and collagenase 3 activating protein , was significantly higher in POLY patients ( p = 0 . 017 0 . 037 ) . bFGF levels did not vary between groups . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Body composition , serum lipids , serum IGF 1 and IGFBP 3 levels were measured during the 24 week study . ^^^ In addition , significantly increased serum IGF 1 and IGFBP 3 levels and improvements in the patients ' serum lipid profiles were observed in patients who received GH therapy . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
We conducted a prospective study within the Nurses ' Health Study 2 to examine the reproducibility of plasma estrogens , androgens , progesterone , prolactin , sex hormone binding globulin , insulin like growth factor 1 ( IGF 1 ) , and IGF binding protein 3 ( IGFBP 3 ) . ^^^ When values were averaged across the follicular and luteal phases , the ICC for prolactin was 0 . 64 whereas ICCs for IGF 1 and IGFBP 3 were 0 . 86 and 0 . 82 , respectively . ^^^ These data suggest that for androgens , estrone sulfate , prolactin , IGF 1 , and IGFBP 3 , a single measurement can reliably categorize average levels over at least a 3 year period in premenopausal women . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In WT mice , the GH antagonist pegvisomant ( Peg ) suppressed the transcription of endogenous IGF 1 and acid labile subunit ( ALS ) genes with no effect on IGF binding protein 3 ( IGFBP 3 ) mRNA . ^^^ Consequently , Peg induced GH deficiency in WT mice severely reduced ALS , IGF 1 , and IGFBP 3 in the circulation and caused a severe growth deficit . ^^^ Interestingly , IGFBP 3 mRNA was elevated and circulating IGFBP 3 was less reduced in Peg treated TTR IGF 1 mice . ^^^ Therefore , liver expressed IGF 1 can stimulate IGFBP 3 mRNA expression and stabilize IGFBP 3 under GH deficiency , leading to a better maintenance of IGF 1 levels in the circulation . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Parallel application of IGFBP 3 had borderline inhibitory effects while the addition of 40ng / mL of IGFBP 5 enhanced the chemotactic effect of IGF 1 on MPC . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The impact of GH deficiency and rhGH replacement therapy on IGF 1 , IGFBP 3 and ALS levels has been widely studied . ^^^ At baseline , GHD patients showed IGF 1 and IGF 2 levels and IGFs to IGFBP 3 molar ratios that were lower than controls . ^^^ These modifications resulted in normalization in IGF 1 to IGFBP 3 ratio , while no change in IGF 2 to IGFBP 3 ratio was observed . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : Pegvisomant effectively reduced IGF 1 and IGFBP 3 levels in gsp mediated GH excess but had no effect on fibrous dysplasia . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The objective of the study was to examine the association between timing of puberty and adulthood serum IGFs ( IGF 1 and IGF binding protein 3 ) . ^^^ MAIN OUTCOME MEASURE : The study measured serum IGF 1 and IGF binding protein 3 at mean age 63 yr . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The high affinity IGF binding proteins ( IGFBP 1 to 6 ) have also been involved in IGF 1 regulation , and it is important to include the IGF independent properties , particularly those of IGFBP 3 that may be involved in the osteoblastic differentiation observed in human bone marrow stromal cell cultures . ^^^ Thus , maternal and umbilical cord serum IGF 1 and 1 , 25 ( OH ) D ( 3 ) concentrations are lower in preeclampsia and umbilical cord serum IGF 1 , IGFBP 1 and IGFBP 3 concentrations are associated with low newborn birth weights . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Overall , plasma IGF 1 , IGFBP 1 , IGFBP 3 , and GH levels were not associated with breast cancer risk ( relative risks , top vs bottom quartile ; IGF 1 , 0 . 98 , 95 % confidence interval ( CI ) , 0 . 69 1 . 39 ; IGFBP 1 , 0 . 95 , 95 % CI , 0 . 63 1 . 41 ; IGFBP 3 , 1 . 10 , 95 % CI , 0 . 78 1 . 54 ; GH , 1 . 09 , 95 % CI , 0 . 82 1 . 46 ) . ^^^ In conclusion , circulating IGF 1 , IGFBP 1 , IGFBP 3 , and GH levels appear to have no important association with breast cancer risk in a large cohort of premenopausal women . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGFBP 3 and breast cancer risk in women : The European Prospective Investigation into Cancer and Nutrition ( EPIC ) . ^^^ Blood concentrations of insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) have recently been associated with breast cancer risk , notably in women who developed breast cancer at a young age . ^^^ We present the results of a large prospective case control study nested within the European Prospective Investigation into Cancer and Nutrition on total IGF 1 , IGFBP 3 and breast cancer risk including 1081 incident cases of invasive breast cancer and 2098 matched control subjects . ^^^ There was , however , significant heterogeneity in the relationship of breast cancer with serum IGF 1 and IGFBP 3 levels depending on the time interval between blood donation and tumor diagnosis . ^^^ This study confirms previous findings for an association of serum IGF 1 and IGFBP 3 concentrations with breast cancer risk , particularly for women with a later diagnosis of cancer , but it does not support the hypothesis of an involvement of IGF 1 in younger women . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF 1 circulates predominantly in multi protein complexes consisting of one molecule each of IGF 1 , IGF binding protein 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Sixteen women patients with anorexia nervosa and thirteen healthy women of comparable age were examined clinically and blood samples were taken for estimation of serum levels of ghrelin , leptin , soluble leptin receptor , IGF 1 , IGFBP 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
AIM : The aim of the present study is : ( 1 ) to evaluate the serum concentrations of leptin and resistin in the paediatric patients with chronic renal impairment ( CRI ) , on haemodialysis ( HD ) and on peritoneal dialysis ( PD ) treatment ; ( 2 ) to examine the relationship between these hormones ; and ( 3 ) to investigate the possible influence of these hormones on the insulin resistance and sensitivity indexes as well as on serum insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : The interplay between GH secretion , NO production , IGF 1 , IGFBP 1 , and IGFBP 3 levels , lipid profile , insulin sensitivity and the degree of visceral obesity necessitates further study . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
RESULTS : All the patients with certain GHD had low ( < or = 2 z score ) plasma concentrations of IGF 1 and ALS , but only 35 . 1 % had low IGF 2 , and 48 . 6 % had low IGFBP 3 . ^^^ All the patients but three ( 83 . 3 % ) with transient GHD had low IGF 1 , but only 44 . 4 % had low ALS , and only one had low IGF 2 or IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Effect of carbamezapine and valproic acid on bone mineral density , IGF 1 and IGFBP 3 . ^^^ OBJECTIVE : To examine the effect of carbamezapine and valproate on bone mineral density ( BMD ) , IGF 1 and IGFBP 3 levels in children . ^^^ Valproic acid and carbamazepine therapy do not change IGF 1 and IGFBP 3 levels . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) is a multifunctional protein that regulates the potent mitogenic and antiapoptotic effects of IGF 1 and IGF 2 and exerts bioactivity independent of modulating IGF receptor activation . ^^^ IGF binding protein 3 ( IGFBP 3 ) is a multifunctional protein that regulates the potent mitogenic and antiapoptotic effects of IGF 1 and IGF 2 and exerts bioactivity independent of modulating IGF receptor activation . ^^^ IGF 1 and heparin blocked binding of IGFBP 3 to matrix when added with the binding protein but were unable to displace IGFBP 3 already bound to the matrix . ^^^ IGF 1 bound to matrix immobilized IGFBP 3 with approximately 25 fold reduced affinity compared with IGFBP 3 in solution . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGFBP 3 in patients with lung cancer during chemotherapy . ^^^ The aim of this study was to assess serum levels of insulin like growth factors ( IGF 1 and IGFBP 3 ) in patients with lung cancer during chemotherapy . ^^^ IGF 1 and IGFBP 3 levels were assessed by ELISA method . ^^^ In conclusion , both before and after chemotherapy serum levels of IGF 1 were significantly higher , whereas IGFBP 3 levels were lower in patients with NSCLC and SCLC compared to controls . ^^^ Chemotherapy had no influence on the serum levels of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOMES : Outcomes included homeostasis model assessment of insulin resistance , total IGF 1 and IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 in adulthood . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Extremely low levels of IGF 1 ( 6 . 9 SDS ) , IGF binding protein 3 ( 12 SDS ) , and acid labile subunit ( 7 . 5 SDS ) were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
OUTCOMES : Outcomes included serum concentrations of E ( 2 ) , ghrelin , GH , IGF 1 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 , and the estimated mass and waveform of stimulated GH secretory bursts . ^^^ Compared with Pl , E ( 2 ) did not alter ghrelin concentrations but reduced IGF 1 and IGFBP 3 and elevated IGFBP 1 concentrations . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Clinical utility of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 measurements in paediatric practice . ^^^ Measurements of serum insulin like growth factor 1 ( IGF 1 ) and its major binding protein , IGFBP 3 , are utilised in the routine clinical management of short children . ^^^ Comment is also made on the relationship between levels of IGF 1 and IGFBP 3 in the population with regard to risks for cancer and cardiovascular disease . ^^^ IGF 1 and IGFBP 3 are important parameters to measure as one part of the process of managing short children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Experimental studies have implicated high levels of IGF 1 directly and IGFBP 3 inversely in prostate cancer growth , survival , and progression . ^^^ However , recent evidence suggests a much weaker association of IGF 1 with prostate cancer development and a stronger antagonistic association of IGFBP 3 with prostate cancer progression . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
GH ) , insulin like growth factor 1 ( IGF 1 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) axis in hypoestrogenic , hypergonadotropic women . ^^^ OBJECTIVE : Ovarian hormonal function may be as important contributing factor to hGH IGF 1 IGFBP 3 axis as age . ^^^ AIM : To examine plasma hGH , IGF 1 and IGFBP 3 levels in women with premature ovarian failure compared to healthy normal controls and postmenopausal ones . ^^^ Basic fasting plasma hGH , IGF 1 , IGFBP 3 , insulin , testosterone and LH as well as prolactin ( PRL ) , FSH and estradiol were assessed by RIA kits . ^^^ In none of groups hGH / IGF 1 as well as IGFBP 3 / hGH relations were found . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Standard clinical evaluation and hormone evaluation ( LH , FSH , prolactin , estradiol , leptin , insulin like growth factor 1 ( IGF 1 ) , human growth hormone ( hGH ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , insulin , dihydroepiandrosterone sulphate ( DHEAS ) , sex hormone binding globin ( SHBG ) and testosterone were done in basic condition which levels of were measured by RIA kits . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In fact , the increase in IGF 1 levels induced by this treatment was paralleled by comparable increases in binding protein ( IGFBP 3 ) . ^^^ The addition of tamoxifen to bicalutamide produced a sharp decrease in IGF 1 levels ( p < 0 . 001 ) coupled with an increase in both IGFBP 1 ( p=0 . 001 ) and , to a lesser extent , IGFBP 3 ( p=0 . 5 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Serum bone alkaline phosphatase , CrossLaps , IGF 1 , and IGF binding protein 3 levels were determined in patients with values analyzed using our normative data from 1150 healthy children . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Pregnancy induced changes in insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) in patients with growth hormone ( GH ) deficiency and excess . ^^^ BACKGROUND : Under most circumstances with altered growth hormone ( GH ) secretion , the changes of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) are in parallel . ^^^ The aim of the present study was to compare the effects of pregnancy in a hypopituitary patient with those of pregnancy in an acromegalic patient on IGF 1 , IGFBP 3 , and ALS . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , serum levels of free and total IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , lipids and HbAlc were measured . ^^^ No differences were observed in lipids , total IGF 1 , IGFBP 3 or HbAlc . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Recombinant human IGF 1 ( rhIGF 1 ) complexed with its natural binding protein IGF binding protein ( IGFBP ) 3 ( rhIGF I / IGFBP 3 ) is a novel formulation that has been shown to improve insulin sensitivity in type 1 diabetes , yet the mechanisms are not clear . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The changes in the serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , insulin like growth factor binding protein 3 ( IGBP 3 ) , estrogen , total alkaline phosphatase ( TALP ) , bone specific alkaline phosphatase ( BALP ) , calcium and phosphorous in serum , and also the histomorphology of the proximal tibia metaphysis and femur / body weight ( F / B ) ratio were examined in all the groups at every 10 days interval . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Inverse correlation between serum free IGF 1 and IGFBP 3 levels and blood pressure in patients affected with type 1 diabetes . ^^^ AIM : Our aim is to analyze the relationship between systolic and diastolic blood pressure and serum free IGF 1 and IGFBP 3 levels in subjects suffering from type 1 diabetes . ^^^ Similar but less significant relationships were observed for IGFBP 3 , whose levels were also significantly and directly correlated with those of free IGF 1 . ^^^ The correlation between systolic and diastolic blood pressures with free IGF 1 and between systolic blood pressure and IGFBP 3 levels were confirmed after adjusting for age , gender , age at diagnosis , disease duration , familial history , HBA1c , and amount of insulin administered by multivariate logistic regression analysis . ^^^ A decrease in free IGF 1 and IGFBP 3 levels , along with increases in blood pressure , significantly influenced the presence of diabetic complications , confirming how these molecules may be considered as severity markers for patients with type 1 diabetes as well as risk factors for altered pressure control linked diseases . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Serum levels of total IGF 1 , free IGF 1 , and IGFBP 3 were measured by radioimmunoassay in 10 patients with ischemic stroke treated with intravenous tPA ( 0 . 9 mg / kg body weight ) and 10 untreated controls . ^^^ RESULTS : During tPA treatment , total IGF 1 and IGFBP 3 serum levels did not change , but there was an & 70 % increase in free IGF 1 serum levels from 0 . 98+ / 0 . 25 at baseline to 1 . 69+ / 0 . 18 nmol / L at the end of the 1 hour infusion ( P=0 . 01 ) . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
The correlation of the IGF 1 , IGFBP 3 , and ALS generation test to height velocity after 6 months of recombinant growth hormone therapy in girls with Turner syndrome . ^^^ Levels of IGF 1 and IGFBP 3 were measured before and 5 , 30 and 90 days after starting hGH ( 0 . 05 mg / kg / day ) , ALS levels were measured only prior and after 5 days . ^^^ Basal ALS values correlated to height velocity SDS and IGF 1 and IGFBP 3 , after 90 days , correlated to height velocity . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
METHODS : Fasting IGFBP 2 , IGFBP 1 , IGFBP 3 , IGF 1 , IGF 2 , insulin , C peptide , glucose , lipids , NEFAs , and HbA1c were measured in a cohort of 163 patients with type 2 diabetes . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In contrast , no important link has been found between circulating levels of IGF 1 ( and its binding protein , IGFBP 3 ) and breast cancer risk in postmenopausal women . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Maternal and mixed umbilical cord blood was collected at delivery and analyzed for insulin , glucose , IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , GH , and GHBP . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
This study aimed to explore the effects of L arginine on the expression of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and IGF 1 mRNA in brains of IUGR rats and the possible mechanisms of L arginine . ^^^ The levels of IGF 1 , IGF 2 and IGFBP 3 in the brains of pup rats were measured by enzyme linked immunoadsordent assay ( ELISA ) and the expression of IGF 1 mRNA was detected by fluorescence quantitative PCR ( FQ PCR ) . ^^^ CONCLUSIONS : L arginine can increase the levels of IGF 1 and IGF 2 and the IGF 1 mRNA expression , and decrease the IGFBP 3 level in the brain of rats with IUGR induced by passive smoking , thereby offering protective effects against IUGR . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
To assess whether circulating IGF 1 , IGF 2 , or IGF binding protein 3 ( IGFBP 3 ) were associated with cervical cancer and low grade and high grade squamous intraepithelial lesions ( LSIL and HSIL ) . ^^^ MATERIALS AND METHODS : Serum levels of IGF 1 , IGF 2 and IGFBP 3 were measured by ELISA . ^^^ RESULTS : Significantly lower values of IGF 1 ( 83 . 9 ng / ml versus 126 . 6 ng / ml , p < 0 . 001 ) and IGF 1 : IGFBP 3 molar ratio ( 0 . 094 versus 0 . 137 , p < 0 . 001 ) were observed among cancer cases , as compared to their control group . ^^^ Women in the highest quartile of IGF 1 and IGF 1 : IGFBP 3 molar ratio were at an 80 % ( OR = 0 . 2 , 95 % CI [ 0 . 06 0 . 61 ] ) and a 77 % ( OR = 0 . 23 , 95 % CI [ 0 . 07 0 . 73 ] ) lower risk of cervical cancer , respectively , compared with women in the corresponding reference category . ^^^ CONCLUSIONS : These data suggest that low values of IGF 1 and IGF 1 : IGFBP 3 molar ratio may be associated with cervical cancer . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Exogenous addition of IGFBP 3 markedly blocked IGF 1 and IGF 2 stimulated cell growth of KYN 2 and HepG 2 cells , and moderately stimulated that of KIM 1 and HAK 1B cells , but no growth of the KYN 1 , KYN 3 and HAK 1A cell lines was observed . ^^^ IGF 1 enhanced the phosphorylation of IGF 1R , Akt and Erk1 / 2 in KYN 2 cells , and coadministration of IGFBP 3 blocked all types of activation by IGF 1 investigated here . ^^^ IGFBP 3 also suppressed IGF 1 induced cell invasion by KYN 2 cells . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
In human RCC cells , we recently showed that insulin like growth factor 1 ( IGF 1 ) has growth promoting effects regulated by IGF binding protein 3 ( IGFBP 3 ) . ^^^ IGF 1 and IGFBP 3 augment transforming growth factor beta actions in human renal carcinoma cells . ^^^ Functional effects such as cell proliferation , TGF beta receptor ( TbetaR ) signaling , and IGFBP 3 levels were monitored after stimulation with various concentrations of IGF 1 , TGF beta , and IGFBP 3 . ^^^ In addition , IGF 1 increased the IGFBP 3 levels five to eightfold with TGF beta acting in synergy to enhance the IGFBP 3 levels 12 to 17 fold . ^^^ The observations that IGF 1 enhances the TGF beta signaling and that TGF beta promotes IGFBP 3 production and thus influence the biological activity of IGF may be of importance for future therapeutic options . . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Furthermore , we have confirmed that breast density was increased among premenopausal women with high levels of IGF 1 and low levels of IGFBP 3 which is consistent with the observed effect of these molecules on breast cancer risk . ^^^
Interacting proteins: P05019 and P17936 Pubmed SVM Score :0.0
Central and opposing effects of IGF 1 and IGF binding protein 3 on systemic insulin action . ^^^ As there is a paucity of IGF 1 receptors in the liver and as the IGF IGFBP system in the central nervous system is emerging as physiologically relevant , we examined whether the effects of IGF 1 and IGFBP 3 on insulin action are mediated through central mechanisms . ^^^ While IGF 1 marginally increased peripheral glucose uptake , IGFBP 3 significantly decreased peripheral glucose uptake ( approximately 30 % , P < 0 . 01 ) . ^^^ Marked , opposing , and independent physiological effects of IGF 1 and IGFBP 3 through central mechanisms may have implications on potential strategies in specific modulation of peripheral insulin action . . ^^^