| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Human endothelial actin binding protein ( ABP 280 , nonmuscle filamin ) : a molecular leaf spring . ^^^ Actin binding protein ( ABP 280 , nonmuscle filamin ) is a ubiquitous dimeric actin cross linking phosphoprotein of peripheral cytoplasm , where it promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| To study the morphologic and functional determinants of the Triton insoluble F actin pool , the distribution and quantity of three candidate regulatory proteins , alpha actinin , tropomyosin ( TM ) , and actin binding protein ( ABP 280 ) , were compared in F actin ( Triton insoluble and Triton soluble ) and G actin pools isolated from basal and chemotactic factor activated human PMNs in suspension , using immunoblots and ionic extraction . ^^^ The results show that : ( 1 ) alpha actinin , actin binding protein 280 , and tropomyosin are localized to TIF and excluded from TSF ; ( 2 ) TM , alpha actinin , and ABP 280 are required to stabilize fractions of Triton insoluble F actin in PMNs ; and ( 3 ) chemotactic factor activation results in release of a fraction of TM from the Triton insoluble F actin pool in temporal association with F actin polymerization in the Triton insoluble F actin pool . ^^^ Shifts in ABP 280 or alpha actinin do not occur . ^^^ The results suggest that TM , alpha actinin , and ABP 280 provide structure to TIF and that TM release from TIF is involved in chemotactic factor induced actin polymerization in PMNs . . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Here we show that the p 90 ribosomal S 6 kinase 2 ( RSK 2 ) phosphorylates actin binding protein ( ABP 280 ) in intact rat 3Y1 fibroblasts . ^^^ Growth factors such as fetal calf serum , epidermal growth factor , phorbol 12 myristate 13 acetate , and lysophosphatidic acid stimulate the phosphorylation of serine residues in ABP 280 in quiescent 3Y1 cells . ^^^ Extracts from 3Y1 cells prepared after stimulation by lysophosphatidic acid , fetal calf serum , and epidermal growth factor retain activated protein kinase activity ( s ) toward ABP 280 in vitro . ^^^ Two dimensional phosphopeptide maps show RSK 2 phosphorylated ABP 280 to be phosphorylated at the same site ( s ) as those stimulated by growth factors in vivo . ^^^ These results show RSK 2 to phosphorylate ABP 280 in vivo . . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| SEK 1 , a dual specificity protein kinase that serves as one of the immediate upstream activators of the stress activated protein kinases ( SAPKs ) , associates specifically with the actin binding protein , ABP 280 , in vitro and in situ . ^^^ SEK 1 binds to the carboxyl terminal rod segment of ABP 280 , upstream of the ABP carboxyl terminal dimerization domain . ^^^ Activation of SEK 1 in situ increases the SEK 1 activity bound to ABP 280 without changing the amount of SEK 1 polypeptide bound . ^^^ The influence of ABP 280 on SAPK regulation was evaluated in human melanoma cells that lack ABP 280 expression , and in stable transformants of these cells expressing wild type ABP , or an actin binding but dimerization deficient mutant ABP ( ABPDeltaCT 109 ) . ^^^ ABP 280 deficient cells show an activation of SAPK in response to most stimuli that is comparable to that seen in ABP 280 replete cells ; ABP 280 deficient cells , however , fail to show the brisk tumor necrosis factor alpha ( TNF alpha ) activation of SAPK seen in ABP replete cells and have an 80 % reduction in SAPK activation by lysophosphatidic acid . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Based on the characterization of the hypotonic cell volume regulatory response of human melanoma cells devoid of a functional actin binding protein ( ABP 280 , a filamin homolog ) and their genetically rescued counterpart transfected with a functional ABP , a hypothesis is raised which is consistent with a regulatory `` sensory ' ' mechanism based on the ability of actin networks to respond to changes in the intracellular water salt homeostasis , which in turn effects signals controlling membrane function , including ion channel activity . . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence of the protein shared a high degree of homology with human endothelial ABP 280 ( 70 % identity ) and chicken filamin ( 83 % identity ) . ^^^ However , the 32 amino acid Hinge 1 region in ABP 280 that contains a calpain cleavage site conferring flexibility on the molecule , was absent in the homologue . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| In this report human epithelial CFTR was expressed in human melanoma cells genetically devoid of the filamin homologue actin cross linking protein ABP 280 [ ABP ( ) ] . cAMP stimulation of ABP ( ) cells or cells genetically rescued with ABP 280 cDNA [ ABP ( + ) ] was without effect on whole cell Cl ( ) currents . ^^^ Further , in cells expressing both CFTR and a truncated form of ABP 280 unable to cross link actin filaments , cAMP was also without effect on CFTR activation . ^^^ Dialysis of ABP 280 or filamin through the patch pipette , however , resulted in a DPC inhibitable increase in the whole cell currents of ABP ( ) / CFTR ( + ) cells . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Modulation of dopamine D ( 2 ) receptor signaling by actin binding protein ( ABP 280 ) . ^^^ In this study , actin binding protein 280 ( ABP 280 ) , a widely expressed cytoskeleton associated protein that plays an important role in regulating cell morphology and motility , was found to associate with the third cytoplasmic loop of dopamine D ( 2 ) receptors . ^^^ The functional significance of the D ( 2 ) receptor ABP 280 association was evaluated in human melanoma cells lacking ABP 280 . ^^^ Further yeast two hybrid experiments showed that ABP 280 association is critically dependent on the carboxyl domain of the D ( 2 ) receptor third cytoplasmic loop , where there is a potential serine phosphorylation site ( S 358 ) . ^^^ This mutant ( D ( 2 ) S358D ) displayed compromised binding to ABP 280 and coupling to adenylate cyclase . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Three different C terminal regions of human endothelial actin binding protein 280 ( ABP 280 or ABP ; nonmuscle filamin ) were subcloned and efficiently expressed in the Escherichia coli BL 21 ( DE 3 ) system as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Decreased glomerular expression of the laminin beta 1 chain , unaltered expression of the laminin beta 2 and gamma 1 chains , and increased expression of the laminin alpha 1 chain and filamin / actin binding protein 280 ( ABP 280 ) were found during disease progression . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| A middle region of human endothelial actin binding protein ( ABP ) was subcloned and expressed in the pT 7 7 / E . coli BL 21 ( DE 3 ) system . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| The development of handling induced seizures also was studied in EL mice , in nonepileptic ABP and DDY mice , and in reciprocal ABP 10 EL F 1 hybrids from ages 30 180 days . ^^^ Furthermore , susceptibility was higher in ABP 10 EL F 1 hybrids than in their reciprocal EL 10 ABP F 1 hybrids at 90 150 days . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In the ATR group there were significant reductions of SBP ( deltaSBP 1 2 : 13 . 7 + / 5 . 6 mmHg , P 0 . 001 ) , DBP ( deltaDBP 1 2 : 7 . 8 + / 5 . 7 mmHg , P 0 . 01 ) , LDL ( deltaLDL 1 2 : 67 . 7 + / 28 . 3 mg / dl , P < 0 . 001 ) and improvement of brachial artery FMD ( deltaFMD 2 1 : 4 . 2 + / 2 . 6 % ) . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| At randomization and at the end of each study phase , mean 24 h systolic BP ( SBP ) and diastolic BP ( DBP ) were assessed by 24 h ambulatory blood pressure monitoring ( ABPM ) and endothelium dependent ( flow mediated , FMD ) and independent ( nitroglycerin induced , NTG ) vasodilatations of brachial artery were measured using high resolution ultrasound . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| SBP decreased more after PTRA among patients with FMD only in the main renal artery than in those with branch artery involvement ( 43+ / 29 vs 20+ / 41 mmHg ; P=0 . 0198 ) . ^^^ |
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| Interacting proteins: P21333 and P04278 |
Pubmed |
SVM Score :0.0 |
| Systolic blood pressure ( SBP ) of MNS . ^^^ H Add 1 rats was significantly higher ( +10 mmHg ) than that of MNS , whereas SBP of MHS . ^^^ |
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