| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :1.1286922 |
| The relationship between IGF 2 and IGFBP 5 was examined further by cotransfecting C 2 myoblasts with antisense Igf 2 ( previously established to induce increased cell death ) and Igfbp 5 ; both wt and mutIGFBP 5 conferred equivalent protection against the decreased cell survival and increased apoptosis . 1.1286922^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Coincubation of IGF 1 or IGF 2 and IGFBP 5 with fibroblast cultures decreased proteolysis . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 2 and insulin also increase IGFBP 5 mRNA levels at high doses . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In C 2 anti IGF 2 myoblasts , IGFBP 5 induction is therefore rapamycin sensitive and independent of differentiation . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The major cell associated binding site for [ 125I ] IGF 1 is IGFBP 3 and for [ 125I ] IGF 2 are IGFBP 3 and IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In contrast , when IGFBP 5 was added in the presence of IGF 2 , it inhibited both proliferation and differentiation . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Notable among this group were the growth factor gene Igf 2 and its binding protein Igfbp 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 2 and IGFBP 5 mRNA levels in control and Dex treated cultures were similar . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGFBP 5 and IGF 2 protein expression correlated with mRNA expression . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Content of IGFII in tumor cells correlated with IGFBP 1 , IGFBP 2 , IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| At neutral pH proteolysis of IGFBP 5 occurred during incubation in conditioned media from control and IGF 2 overexpressing cells . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Gene products characteristic of the proliferation state , such as c Jun , continued to accumulate in the mutant cells in low serum , whereas those involved in differentiation , like myogenin , insulin like growth factor 2 ( IGF 2 ) , and IGF binding protein 5 ( IGFBP 5 ) were undetectable . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 1 , IGF 2 , and IGF binding protein 5 ( IGFBP 5 ) mRNAs were measured with a ribonuclease protection assay in the gastrocnemius of specific pathogen free Fischer 344 rats . ^^^ We hypothesized that IGF 1 , IGF 2 , and IGFBP 5 mRNA concentration ( normalized to 18S RNA ) in the gastrocnemius muscle of growing animals ( 3 mo ) would be downregulated in a coordinated manner with muscle size during aging associated atrophy . ^^^ We conclude that the aging associated atrophy of skeletal muscle is not caused by altered pretranslational regulation of IGF 1 , IGF 2 , or IGFBP 5 in skeletal muscle . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Proteolysis of the 29 kilodalton ( kDa ) [ 125I ] IGFBP 5 produced an 18 to 20 kDa fragment of IGFBP 5 , and 25 kDa [ 125I ] IGFBP 4 yielded two lower mol wt fragments in the presence of IGF 2 . ^^^ CM from IGF 2 treated U2OS and normal HBC cultures exhibited decreased IGFBP 5 proteolytic activity compared to control cultures . ^^^ On the contrary , exogenous IGF 2 had no effect on IGFBP 5 proteolysis in cell free U2OS CM . ^^^ Based on the findings that HBCs in culture produce a protease ( s ) capable of cleaving both IGFBP 4 and IGFBP 5 and that IGF 2 can promote or inhibit proteolytic degradation of IGFBP 4 and IGFBP 5 , respectively , it is proposed that IGFBP protease ( s ) may be an important modulator of IGF activity in bone . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The concentrations of both IGFBPs were increased by the addition of IGF 1 , IGF 2 , or insulin to the cell cultures , with IGFBP 5 demonstrating the greatest hormonal stimulation . ^^^ The effects of IGF 1 on IGFBP 5 expression were both time and dose dependent , with IGF 1 being more potent than IGF 2 , and IGF 2 more potent than insulin . ^^^ Similarly , the IGF 2 analog [ Leu 27 ] IGF 2 , which has very low affinity for the type 1 receptor , only slightly stimulated an increase in IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To determine whether IGFs may participate in the initiation of restorative reactions in inactivated muscle we analyzed the expression of IGF 1 and IGF 2 mRNA in botulinum toxin paralyzed and in denervated rat skeletal muscle , and interfered with the activity of IGFs by locally applying the IGF binding proteins IGF BP 5 and IGF BP 4 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization analysis revealed that IGF 2 , IGFBP 5 , and IGFBP 6 mRNAs were localized in the stromal component of the tumor , whereas IGFBP 2 mRNA was expressed by epithelial cells . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In this study we have exploited the high affinity and specificity of IGF binding protein 4 ( IGF BP 4 ) and IGF BP 5 for IGF 1 and IGF 2 to determine whether these growth factors are involved in the nerve sprouting reaction in paralyzed skeletal muscle . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In contrast , treatment with IGF 2 or [ Leu 27 ] IGF 2 , type 2 IGF receptor selective ligands , yielded a more limited effect on IGFBP 5 accumulation in keeping with an overall rank order of potency of des ( 1 3 ) IGF 1 > IGF 1 > IGF 2 > or = [ Leu 27 ] IGF II . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 1 and IGF 2 had no effect on IGFBP 2 steady state transcript levels but enhanced the level of IGFBP 5 transcripts ( approximately fourfold ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We conclude that prostatic stromal cell strains isolated from patients with BPH hyperexpress the mRNA for IGF 2 and IGFBP 5 while expressing reduced amounts of IGFBP 2 mRNA . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| These findings support the view that the IGF system plays a fundamental role in endometrial biology , acting via autocrine and / or paracrine mechanisms , with IGF 1 and IGFBP 5 being dominant in the proliferative phase , and IGF 2 and the other IGFBPs predominant in the secretory phase of the menstrual cycle . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Loss of detectable membrane associated IG FBP 5 in transformed cells was associated with diminished IGFBP 5 gene expression and with loss of IGF 2 gene expression . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Upon formation of the S shape nephron , IGFBP 2 mRNA was expressed in the committed glomerular and epithelial cells which also expressed IGF 2 and IGFBP 4 , and the mesenchyme of the vascular cleft expressed IGFBP 5 mRNA . ^^^ In the maturing glomerulus , the glomerular epithelial cells expressed IGF 2 mRNA together with IGFBP 2 and IGFBP 4 mRNAs , while IGFBP 5 mRNA was localized to the mesangium and supporting mesenchyme . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 1 and IGF 2 treatment elevated IGFBP 5 in conditioned media , but longR3IGF 1 and insulin , which do not bind to IGFBPs , had smaller effects . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Whereas IGF 2 expression was already increased by experimental stress , IGFBP 5 mRNA was enhanced only by ischaemia / reperfusion . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In this study , we describe the mRNA expression of IGF 1 , IGF 2 , type 1 IGF receptor , IGFBP 2 , IGFBP 4 and IGFBP 5 during mouse lung development as studied by in situ hybridization techniques . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The validity of the IGFBP 5 RIA was established from the following data : 1 ) recombinant human IGFBP 5 and purified human bone derived IGFBP 5 inhibited the binding of tracer to the antisera in an identical manner ; 2 ) the immunoreactive IGFBP 5 in serum co migrated with recombinant human IGFBP 5 in both sodium dodecyl sulfate polyacrylamide gel electrophoresis and high performance gel filtration chromatography ; 3 ) none of the other known IGFBPs ( IGFBP 1 , 2 , 3 , 4 , and 6 ) exhibited significant cross reactivity ; 4 ) the addition of IGF 1 or IGF 2 to the samples did not affect the recovery of IGFBP 5 ; and 5 ) the recovery of the exogenously added IGFBP 5 to serum was greater than 90 % . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Conditioned media IGFBP 5 increased in response to IGF 1 , IGF 2 , and insulin in a dose dependent fashion , compared with unstimulated FRTL 5 cells . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Since extracellular matrix ( ECM ) has been shown to be a reservoir for IGF 1 and IGF 2 , we examined the ECM of cultured human fetal fibroblasts and found that IGFBP 5 was incorporated intact into ECM , while mostly inert proteolytic fragments were found in the medium . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 1 , IGF 2 , and the IGF 1 analog [ 1 27Gly ( 4 ) 38 70 ] IGF 1 bound IGFBP 5 with high affinity and , when added to U 2 cultures , effectively promoted IGFBP 5 accumulation in the medium . ^^^ Cell free incubation of recombinant human ( rh ) IGFBP 5 in U 2 conditioned medium resulted in a marked reduction of detectable rhIGFBP 5 ; the presence of IGF 1 or IGF 2 peptide partially prevented this decrease . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Studies comparing IGF 1 , IGF 2 , different IGF 1 analogs , and insulin indicated that the predominant IGF effect on IGFBP 5 accumulation was type 1 IGF receptor independent . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 2 and IGF binding protein 5 and 6 ( IGFBP 5 and 6 ) mRNAs are abundant in mesenchymal condensations and chondroblasts on E 15 . ^^^ Chondrocyte IGF 2 mRNA levels remain high , but IGFBP 5 and 6 mRNAs decline significantly as cartilage matures . ^^^ Interestingly , IGF 2 , both IGF receptors , and IGFBP 5 and 6 are simultaneously coexpressed in chondrocyte precursors early in skeletal development , suggesting functional interactions between these specific factors in chondrogenesis . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The EGF induced mitogenesis was abrogated in a dose dependent manner by IGF binding protein 5 ( IGFBP 5 ) , which binds to IGF 2 and neutralizes it . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with IGF 1 and IGF 2 resulted in a dose dependent increase of IGFBP 5 and a small increase in IGFBP 4 in conditioned media ( CM ) . ^^^ Des ( 1 3 ) IGF 1 and [ Gln 6 , Ala 7 , Tyr 18 , Leu 19 ] IGF 2 ( [ QAYL ] IGF 2 ) , which bind to the type 1 IGF receptor but not to IGFBPs , also induced IGFBP 5 peptide , although the increase was less than with IGF 1 or IGF 2 treatment of RAC . [ Leu 27 ] IGF 2 , which does not bind to the type 1 IGF receptor but binds to IGFBPs , resulted in little induction of IGFBP 5 , while [ QAYL Leu 27 ] IGF 2 , which has reduced affinity for both the type 1 IGF receptor and IGFBPs , did not increase IGFBP 5 . ^^^ Northern blotting analysis showed that IGF 1 , IGF 2 and des ( 1 3 ) IGF 1 treatment of RAC increased steady state levels of IGFBP 5 mRNA , suggesting that the IGF mediated increase in IGFBP 5 is transcriptionally modulated . ^^^ IGFBP 5 protease activity was detectable in untreated CM , whereas treatment with IGF 1 and IGF 2 partially protected IGFBP 5 from proteolysis . ^^^ In summary , treatment of RAC with IGF 1 and IGF 2 results in dose dependent increases in both IGFBP 5 peptide in the CM and mRNA levels . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGFBP 5 mRNA expression is stimulated in normal bone cells by parathyroid hormone . 30 nM IGFBP 5 stimulates 3H thymidine incorporation in calvaria ( which produce IGF 1 contributing to basal proliferation in serum free medium ) but not in Saos 2 cells which produce little IGF 1 and IGF 2 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| These age related changes in the serum levels of IGF system components are consistent with our previous findings of age related decreases in the femoral cortical contents of IGF 1 , IGF 2 and IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Accelerated differentiation of IGF 2 overexpressing C 2 cells was preceded by the rapid induction of myogenin mRNA and protein expression ( within 1 h , compared with 24 48 h in controls ) and was accompanied by an enhanced proportion of the retinoblastoma protein in an underphosphrylated and potentially active form , by a marked increase in activity of the muscle specific enzyme , creatine phosphokinase , by extensive myotube formation by 48 h , and by elevated secretion of IGF binding protein 5 when compared with controls . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To investigate the contribution of the insulin like growth factors ( IGFs ) and their binding proteins ( IGFBPs ) to the regulation of bone growth in 10 GH deficient Japanese children receiving recombinant GH therapy , we determined the percent increase from pretreatment levels of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , and bone specific alkaline phosphatase isoenzyme ( B ALP ) . ^^^ The elevation of IGF 1 induced by GH was significantly correlated with the increases in IGFBP 3 ( r = 0 . 735 ; P < 0 . 0001 ) and IGFBP 5 ( r = 0 . 795 ; P < 0 . 0001 ) , and the elevation of B ALP was also significantly positively correlated with the increases in IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 ( r = 0 . 544 , P < 0 . 0001 ; r = 0 . 268 , P = 0 . 0399 ; r = 0 . 414 , P = 0 . 0010 ; and r = 0 . 500 , P < 0 . 0001 , respectively ) . ^^^ Our data are consistent with the anabolic effect on bone growth of GH treatment being mediated by IGF 2 , IGFBP 3 , and IGFBP 5 as well as by IGF 1 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The expression of IGF 2 precedes the expression of its binding protein 5 ( IGFBP 5 ) . ^^^ The protective effect of IGF 2 was lost when IGFBP 5 was simultaneously infused . ^^^ IGFBP 5 , whose expression follows IGF 2 ' s expression with a short delay , inhibits the cardioprotection afforded by IGF 2 and may thus account for the limited temporal duration of ischemic preconditioning . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The mitogenic effect of tryptorelin was inhibited when cells were co incubated with either IGF binding protein 5 ( IGFBP 5 ) or anti IGF 2 antibody , which can both bind to IGF 2 and neutralize it . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that secretion of IGFBP 4 and IGFBP 5 by L 6 and BC3H 1 muscle cells was stimulated by treatment with either insulin , IGF 1 , or IGF 2 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Osteogenic protein 1 regulates insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 5 ( IGFBP 5 ) gene expression in fetal rat calvaria cells by different mechanisms . ^^^ Previous studies from our laboratory revealed that OP 1 led to a two to threefold increase in steady state insulin like growth factor 1 ( IGF 1 ) and IGF 2 mRNA levels and a fivefold decrease in IGF binding protein 5 ( IGFBP 5 ) mRNA levels in primary cultures of fetal rat calvaria ( FRC ) cells . ^^^ In conclusion , OP 1 regulates IGF 1 gene expression at the posttranscriptional level , but regulates IGF 2 and IGFBP 5 gene expression at the transcriptional level . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate using three independent methods that human IGFBP 5 , when occupied by IGF 1 or IGF 2 , forms ternary complexes of approximately 130 kDa with the acid labile subunit . ^^^ As observed for IGFBP 3 , ternary complexes containing IGFBP 5 form preferentially in the presence of IGF 1 , even though IGFBP 5 has a preferential affinity for IGF 2 over IGF 1 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Competitive binding studies , using either [ 125I ] IGF 1 or [ 125I ] IGF 2 , indicated that La3+ and Cr3+ did not affect [ 125I ] IGF 1 or [ 125I ] IGF 2 binding to cell associated IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The injured sciatic nerve expressed IGF 1 and IGF 2 as well as IGFBP 4 and IGFBP 5 , whereas central nervous system ( CNS ) scar tissue expressed IGF 1 , IGFBP 2 , and IGFBP 5 . ^^^ In line with the in vivo findings , cultured Schwann cells expressed IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 5 mRNAs , whereas cultured astrocytes expressed IGF 1 , IGFBP 2 , and IGFBP 5 mRNAs . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Whereas 6 6 5 , like IGFBP 6 , had a marked preference for binary complex formation with IGF 2 rather than IGF 1 , it formed ternary complexes more efficiently with IGF 1 , like IGF BP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In the chronic phase of injury ( 7 14 dpl ) , an increase in both IGF 2 , IGF IIR and IGFBP 5 messenger RNA and protein was observed specifically and focally in the marginal astrocytes forming the limiting glial membrane of the wound . ^^^ Later in the wounding response , levels of IGF 2 decline in the CSF and the wound neuropil , possibly with the aid of increased IGFBP 5 levels that may help to locally sequester and down regulate IGF 2 activity . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 5 , and total protein were determined in extracts obtained after ethylenediamine tetraacetate and guanidine hydrochloride extraction . ^^^ Cortical bone contents of IGF 1 , IGF 2 , and IGFBP 5 were significantly elevated in the acromegalic patients compared with control values [ 91 % ( P < 0 . 001 ) , 44 % ( P < 0 . 04 ) , and 115 % ( P < 0 . 004 ) , respectively ] . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| At 15 days of gestation , IGF 2 mRNA was expressed in ectoplacental mesoderm , cytotrophoblasts and syncytiotrophoblast , and IGFBP 5 mRNA was detected in the syncytiotrophoblast . ^^^ In the mid gestation placenta , IGFBP 5 mRNA was expressed in the marginal and interlobular syncytium and IGF 2 mRNA in the labyrinth . ^^^ Near term , when expansion of the labyrinth was complete , IGFBP 5 mRNA was coexpressed with IGF 2 mRNA in the marginal and interlobular syncytium . ^^^ These observations suggest that interaction between IGF 2 and IGFBP 5 plays a role in the vascularization of the placenta by fetal vessels . ^^^ IGF 2 mRNA was detected in trophoblasts invading the walls of maternal vessels , and the endothelium of the preplacental vessels expressed IGFBP 4 mRNA , while IGFBP 2 and IGFBP 5 mRNAs were present in the tunica media of mesometrial arteries that had not been invaded by trophoblast . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| A quantitative PCR measurement of messenger RNA expression of IGF 1 , IGF 2 and IGFBP 5 in human skeletal muscle . ^^^ IGF 1 , IGF 2 and IGFBP 5 mRNA are present in adult human skeletal muscle , but no significant changes between meal groups were observed for IGF 1 , IGF 2 or IGFBP 5 mRNA levels , indicating that the expression of these genes are not altered acutely by nutrients and insulin . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Serum IGF 1 ( 25 microg / l ) , IGF 2 ( 194 microg / l ) , IGFBP 3 ( 0 . 4 mg / l ) , and IGFBP 5 ( 87 microg / l ) levels were low compared to age matched prepubertal children . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGFBP 5 production by GT 1 7 cells was stimulated by both IGF 1 and IGF 2 in a dose dependent manner with approximately equal potency , whereas insulin caused no significant effect . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Like insulin like growth factor binding protein 3 ( IGFBP 3 ) , IGFBP 5 forms a ternary complex with insulin like growth factor ( IGF ) 1 or IGF 2 , and the acid labile subunit ( ALS ) . ^^^ In contrast to the latter , IGFBP 5 ( 1 169 ) preferentially formed ternary complexes with IGF 2 rather than IGF 1 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Down regulation of the serum stimulatory components of the insulin like growth factor ( IGF ) system ( IGF 1 , IGF 2 , IGF binding protein [ BP ] 3 , and IGFBP 5 ) in age related ( type 2 ) femoral neck osteoporosis . ^^^ Circulating concentrations of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 , IGFBP 5 , 25 hydroxycholecalciferol ( 25 ( OH ) D 3 ) , and intact parathyroid hormone ( PTH ) were measured in 50 elderly women after sustaining a hip fracture and in 50 healthy age matched controls . ^^^ No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^ In pooled data from the normal and osteoporotic populations , age adjusted multiple regression models based on IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 were found to be highly predictive of serum OC ( R 2 = 19 % , p < 0 . 001 ) and BMD of femoral neck ( R 2 = 49 % , p < 0 . 0001 ) , consistent with an effect of the anabolic IGF components on overall bone formation rate . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To test the hypothesis that impairment in bone formation in renal osteodystrophy in adults with chronic renal failure ( CRF ) might be mediated in part by alterations in circulating levels of the insulin like growth factor ( IGF ) system components , we compared serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 and IGFBP 5 in adults with CRF ( CRF patients with parathyroid hormone ( PTH ) < 100 pg / ml , PTH > 300 pg / ml and end stage renal failure ( ESRF ) patients ) versus age matched controls . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Histologic parameters of bone formation showed significant positive correlations with serum levels of IGF 1 , IGF 2 , and IGFBP 5 . ^^^ CONCLUSION : Patients with primary and secondary hyperparathyroidism showed lower levels of the putative stimulatory IGFBP 5 but higher levels of IGFBP 1 , 2 , 3 , and 6 , whereas total IGF 1 and IGF 2 levels were not or only moderately increased . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The release of IGFBP 2 and IGFBP 5 were also potentiated without changes to steady state mRNA , and for IGFBP 5 this was due in part to a release from the cell membrane in the presence of IGF 2 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Because insulin like growth factor binding protein 5 ( IGFBP 5 ) and insulin like growth factor 2 ( IGF 2 ) are potent regulators of myoblast proliferation and differentiation , we monitored the ability of exogenous TNF alpha to manipulate this system . ^^^ All treatments suppressed IGF 2 production but only TNF alpha blocked IGFBP 5 secretion . ^^^ Suppression of both IGFBP 5 and IGF 2 secretion may explain why of all triggers tested , only TNF alpha not only blocked differentiation , but also promoted cell death . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| These samples were used to study binary complex formation with IGFBPs 1 to 6 using competitive IGF 2 binding assays and ternary complex formation with IGFBP 3 and IGFBP 5 . ^^^ In contrast , ALS bound similarly to IGFBP 5 in the presence of pro IGF 2 and mature IGF 2 . ^^^ These studies suggest that pro IGF 2 preferentially forms binary complexes with IGFBPs , and ternary complexes with IGFBP 5 , rather than ternary complexes with IGFBP 3 as seen predominantly in normal serum . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In guinea pig , where IGFBP 5 mRNA is expressed in the marginal and interlobular syncytium and IGF 2 mRNA in the labyrinth , interaction between IGF 2 and IGFBP 5 mRNA may be involved in vascularization of the placenta by fetal vessels . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| It relates to cell death by differentiation , is followed by accumulation of c fos , c myc , Bax , IGF 2 , IGFBP 2 , IGFBP 5 this indicating their involvement in the regulation of this process . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In contrast to intact human IGFBP 3 and IGFBP 5 , this form of bovine IGFBP 3 forms ternary complexes preferentially with IGF 2 rather than IGF I . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To eliminate possible actions of IGF 2 , if any was produced by osteoblasts derived from IGF 1 knockout mice , the IGFBP 5 effect was studied in the presence of exogenously added IGFBP 4 , a potent inhibitor of IGF 2 actions in bone cells . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Murine C 2 myoblasts differentiate when placed into low serum media ( LSM ) , accompanied by increased IGF 2 and IGF binding protein 5 ( IGFBP 5 ) production . ^^^ Addition of 20 ng / ml TNF alpha on transfer into LSM blocked differentiation , IGF 2 and IGFBP 5 secretion and induced apoptosis . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The proteolytic activity against IGFBP 6 differed at least from IGFBP 5 protease activity in its sensitivity both to IGF 2 and to the hydroxamic acid based disintegrin metalloprotease inhibitor , as well as serine protease inhibitors . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The expression of IGF 1 , IGF 2 , IGF binding protein 5 ( IGFBP 5 ) , and CTGF ( IGFBP rP 2 ) was seen in a higher proportion of mononuclear cells of patients with ALL than in controls . ^^^ Malignant lymphoblasts of T cell origin more often expressed IGFBP 2 and IGFBP 5 , whereas IGF 2 and IGFBP 3 expression was seen more often in lymphoblasts of B cell origin . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Decreased trabecular bone biomechanical competence , apparent density , IGF 2 and IGFBP 5 content in acromegaly . ^^^ However , IGF 2 and IGFBP 5 content was decreased ( P < 0 . 001 and P < 0 . 05 , respectively ) . ^^^ Furthermore , we demonstrate decreased trabecular bone content of IGF 2 and IGFBP 5 in these patients . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Complementary expression of IGF 2 and IGFBP 5 during anterior pituitary development . ^^^ Messenger RNA expression of members of the insulin like growth factor signaling system , IGF 2 and IGFBP 5 , was found in the alphaT 1 1 precursor cell line , but not in the more differentiated cell line , alphaT 3 1 . ^^^ Expression of IGF 2 and IGFBP 5 mRNAs was both temporally and spatially regulated during pituitary development . ^^^ IGF 2 was highly expressed in the epithelium surrounding Rathke ' s pouch at e10 . 5 , while IGFBP 5 expression was restricted to the adjacent oral epithelium . ^^^ At e11 . 5 ( represented by alphaT 1 1 ) , IGF 2 was expressed throughout the pouch , but was coexpressed with IGFBP 5 and alpha subunit in the ventral portion of the pouch epithelium . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| These results indicate that IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 5 are produced by equine granulosa cells and that insulin , FSH , and estradiol play a role in the regulation of steroidogenesis and the IGF system of equine granulosa cells . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 5 , osteocalcin , OPG , RANKL , and total protein were determined in extracts obtained after EDTA and guanidine hydrochloride extraction . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We found transcriptional upregulation of both IGF 1 and IGF 2 in dystrophic muscle , however the possible beneficial effects of the growth factors appear offset by transcriptional upregulation of inhibitory IGF binding proteins and regulators ( IGFBP 2 , 4 , 6 and 7 ; and PRSS 11 [ IGFBP 5 protease ] ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF binding protein 5 , IGF 2 , and insulin receptor substrate 1 all displayed altered patterns and levels of expression in C / EBPbeta ( null ) mice , suggestive of a change in the IGF signaling axis . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| To evaluate whether changes in these proteins may to some extent explain the decrease in bone mass in postmenopausal or senile osteoporosis , we measured bone contents of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 5 , and OPG in combined extracts obtained after EDTA and guanidine hydrochloride extraction in 60 postmenopausal women aged 47 74 ( mean , 63 ) yr with a previous distal forearm fracture and a hip or spine Z score less than 0 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In normal testes a clear expression of IGF 1 , IGF 2 , IGF IR , IGFBP 2 , IGFBP 4 and IGFBP 5 was found . ^^^ The expression of AR , IGF 2 and IGFBP 2 , IGFBP 3 , IGFBP 5 , and 5alpha reductase type 2 did not differ among the different types of tumors . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We report that these genes fall into two distinct groups with respect to expression pattern , with IGFBP 2 displaying broad overlap of mRNA expression with IGFR and IGF 1 during early development , whereas the expression profile of IGFBP 5 most closely resembled that of IGF 2 . ^^^ A striking observation concerning the expression profiles of both IGF 2 and IGFBP 5 at early stages of chick embryogenesis is that both these genes are expressed asymmetrically in a pattern similar to that of Sonic Hedgehog ( Shh ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In contrast , levels and / or sites of expression of IGF 1 , IGF 2 , IGFBP 4 , IGFBP 5 and type 2 receptor in follicular cells strongly differ between mammalian species , suggesting that these phenomena might play species specific or secondary roles in ovarian folliculogenesis . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Human fibroblasts , glioblastoma cells , and murine myoblasts were used to determine the kinetics of IGF 2 binding to cell surface IGFBP 3 , IGFBP 5 , and the IGF 1R , respectively . ^^^ Zn2+ , Cd2+ , and Au3+ , but not La3+ , decreased total binding and the affinity for [ 125I ] IGF 2 association with IGFBP 3 and IGFBP 5 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The transcripts of IGF 1 , IGF 2 , IGF receptor ( IGF R ) , two IGF binding proteins ( IGFBP 2 and IGFBP 5 ) , GH receptor ( GH R ) and insulin receptor ( 1 R ) were measured by RT PCR at 4 , 8 , 12 and 16 weeks of age in the ovaries of ad libitum fed and feed restricted hens . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In the current study , the soluble IGFBP 5 secreted by BC ( 3 ) H 1 cells is shown to bind approximately 50 % more [ ( 125 ) 1 ] IGF 2 than [ ( 125 ) 1 ] IGF 1 at pH 7 . 4 . ^^^ Zn ( 2+ ) is shown to depress the binding of both IGF 1 and IGF 2 to soluble secreted IGFBP 5 ; [ ( 125 ) 1 ] IGF 1 binding is affected more so than [ ( 125 ) 1 ] IGF 2 binding . ^^^ By depressing the association of the IGFs with soluble IGFBPs , Zn ( 2+ ) is shown to repartition either [ ( 125 ) 1 ] IGF 1 or [ ( 125 ) 1 ] IGF 2 from soluble IGFBP 5 onto cell surface IGF receptors . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The soft diet treatment induced a 56 % decrease in IGF 2 mRNA ( P < 0 . 05 ) , a 21 % increase in IGFR 2 mRNA ( P < 0 . 01 ) , and a 38 % decrease in IGFBP 5 mRNA ( P < 0 . 01 ) . ^^^ Thus , IGF 1 , IGF 2 , IGFR 2 , and IGFBP 5 seem to play a role in the atrophy of mouse masseter muscle in response to the change from a hard to a soft diet in an autocrine and / or paracrine manner . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Zinc ( Zn ( 2+ ) ) , a multifunctional micronutrient , was recently shown to lower the affinity of cell associated insulin like growth factor ( IGF ) binding protein ( IGFBP ) 3 and IGFBP 5 for both IGF 1 and IGF 2 , but to increase the affinity of the cell surface type 1 IGF receptor ( IGF 1R ) for the same two ligands . ^^^ Zn ( 2+ ) , at physiological levels , depressed binding of both IGF 1 and IGF 2 to IGFBP 5 , affecting ( 125 ) 1 IGF 1 more than ( 125 ) 1 IGF 2 . ^^^ Both ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 bound to high and low affinity sites on IGFBP 5 . ^^^ Soluble IGFBP 5 and IGFBP 4 depressed the binding of ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 to the IGF 1R , but did not affect binding of ( 125 ) 1 R ( 3 ) IGF 1 . ^^^ By depressing the association of the IGFs with soluble IGFBP 5 , Zn ( 2+ ) partitioned ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 from soluble IGFBP 5 onto cell surface IGF 1Rs . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| These changes may be mediated by insulin like growth factors ( IGF 1 and IGF 2 ) , but the matrix bound binding protein , IGFBP 5 has not been investigated . ^^^ OBJECTIVES : To measure IGF 1 , IGF 2 , and IGFBP 5 in femoral head bone from non OA controls and patients with OA , and to relate these to apparent density ( rhoA ) and elastic modulus ( Ec ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| MEASUREMENTS : We analysed the strength and independence of associations of angiographically assessed presence of CHD with BMI , systolic and diastolic blood pressure , total , high density lipoprotein ( HDL ) and LDL cholesterol , triglycerides , lipoprotein ( a ) , apolipoproteins A 1 and B , total and free IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 5 , acid labile subunit ( ALS ) , insulin , C peptide , testosterone , DHEAS and sex hormone binding globulin . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In large follicle ( > 7 . 9 mm ; Experiment 2 ) granulosa cells , 100 ng / ml of IGF 1 increased ( P < 0 . 05 ) IGFBP 2 mRNA levels above controls and 3 ng / ml of IGF 1 ; 100 ng / ml of IGF 1 also decreased ( P < 0 . 10 ) IGFBP 5 mRNA levels compared to 3 ng / ml of IGF 1 or FSH or 100 ng / ml leptin , while 100 ng / ml of IGF 2 had no effect ( P > 0 . 10 ) on IGFBP 2 , 3 , 4 , and 5 mRNA levels ( Experiment 2 ) . ^^^ In contrast , IGF 1 but not IGF 2 increased ( P < 0 . 01 ) thecal IGFBP 5 mRNA levels . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Here , we present evidence that the expression of IGFBP 5 is a common feature of neuroblastoma cell lines and that IGFBP 5 acts in concert with IGF 2 in inducing cell proliferation . ^^^ Given the relevance of IGF 2 in determining the proliferative and metastatic behavior of NB , the role of IGFBP 5 as a modulator of the IGF signal transduction pathway should be studied further for potential therapeutic applications . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| The potential biological significance of our data is highlighted by the demonstration that IGF 1 and IGF 2 can displace endogenous IGFBP 5 from monolayer cultures of the mouse mammary epithelial cell line HC11 . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Expression of IGF 1 , IGF 2 , and IGF binding protein 5 mRNA was assessed by real time reverse transcription polymerase chain reaction . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 2 , type 1 IGF R and IGFBP 2 , 3 , 4 , and 6 mRNA expression significantly decreased as follicles progressed from healthy to atretic status ( P < 0 . 01 ) , whereas gene expression for type 2 IGF R and IGFBP 5 was greater in atretic follicles ( P < 0 . 01 ) . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| No treatment effect was found for muscle IGF 1 ( P = 0 . 36 ) , IGF 2 ( P = 0 . 51 ) , IGFBP 3 ( P = 0 . 70 ) , or IGFBP 5 ( P = 0 . 51 ) mRNA abundance . ^^^ Uterine IGF 1 ( P = 0 . 46 ) , IGF 2 ( P = 0 . 40 ) , IGFBP 3 ( P = 0 . 29 ) , and IGFBP 5 ( P = 0 . 35 ) mRNA abundance did not differ between treatments . ^^^ Placental IGF 1 ( P = 0 . 30 ) , IGF 2 ( P = 0 . 18 ) , IGFBP 3 ( P = 0 . 94 ) , and IGFBP 5 ( P = 0 . 42 ) mRNA abundance did not differ between treatments . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We present evidence that the MMP 7 cleavage of IGFBP 5 liberates IGF 2 that functions as an autocrine myofibroblast growth factor . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In a rat osteoblast cell culture that we studied , IGF 2 and IGFBP 2 were the most abundant peptides of the IGF system released in cell medium , IGFBP 5 was abundantly expressed but bound to cell surface and cell matrix and IGF 1 and IGFBP 3 were found at very low concentrations . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| We investigated the actions of DHT , T , DHEA , and estradiol ( E 2 ) on insulin like growth factor ( IGF ) 1 , IGF 2 , IGF 1 receptor ( R ) , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 5 in primary cultures of human prostatic stromal cells by assessing cell proliferation , mRNA expression , and protein secretion by MTT growth assay , quantitative real time PCR , and ELISA , respectively . ^^^ None of the four steroids significantly affected IGF IR , IGF 2 , or IGFBP 5 mRNA levels or stromal cell proliferation . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| In fast muscle , there was a decrease in IGF 2 ( P < 0 . 01 ) on embryonic day ( ED ) 16 and an increase in IGFBP 2 ( P < 0 . 01 ) and IGFBP 4 ( P < 0 . 05 ) and in IGFBP 5 ( P < 0 . 05 ) expression on ED 18 in the stimulated group compared with the control group . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| Knockdown of IGFBP 5 by small interfering RNA or immunoneutralization of IGF 2 , abolished myofibroblast responses to MMP 7 . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| IGF 2 , IGFBP 5 or 6 ) within lacunae and in cementoblasts showed an involvement in the resorption repair sequence , which is considered to be a coupling process as known from bone . . ^^^ |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01344 and P24593 |
Pubmed |
SVM Score :0.0 |
| NA |
|