| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In this study we have exploited the high affinity and specificity of IGF binding protein 4 ( IGF BP 4 ) and IGF BP 5 for IGF 1 and IGF 2 to determine whether these growth factors are involved in the nerve sprouting reaction in paralyzed skeletal muscle . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that the actions of IGF 2 in bone are determined not only by its concentration , but also by the concentration of IGFBP 4 as well as other IGFBPs . ^^^ In this study , we sought to determine by Western ligand blotting the effects of growth hormone , IGF 1 and IGF 2 on the production of IGFBP 3 and IGFBP 4 in TE 89 human osteosarcoma cells and in untransformed normal human bone cells derived from rib . ^^^ IGF 1 and IGF 2 , which increased human bone cell proliferation , decreased the level of IGFBP 4 ( 30 % of control at 100 micrograms / l IGF 1 and IGF 2 ) but increased the level of IGFBP 3 ( 3 10 fold at 100 micrograms / l IGF 1 and IGF 2 ) after 48 h of treatment in the conditioned medium of both low and high density TE 89 cell cultures . ^^^ Similar changes in IGFBP 3 and IGFBP 4 levels were also seen in the conditioned medium of human bone cells derived from rib after treatment with IGF 1 and IGF 2 . ^^^ Studies to determine the underlying molecular mechanisms by which IGF 2 decreased the amount of IGFBP 4 in the conditioned medium revealed that IGF 2 decreased the IGFBP 4 mRNA abundance and increased the IGFBP 3 mRNA abundance in human bone cells . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGFBP 4 levels were diminished 54 % and 73 % by 100 ng / ml IGF 1 and IGF 2 , respectively , with no response to insulin . ^^^ IGF 1 , IGF 2 , and insulin all stimulated an approximately 50 % increase in IGFBP 4 concentrations . ^^^ To test the hypothesis that IGF induced alterations in IGFBP 3 and IGFBP 4 concentrations were regulated via the type 1 IGF receptor , we attempted to block IGFBP changes with type 1 IGF receptor antibody alpha IR 3 and to induce IGFBP changes with an IGF 2 analog , [ Leu 27 ] IGF 2 , with little affinity for the type 1 receptor . alpha IR 3 failed to block either the IGF induced rise in IGFBP 3 in each cell line or the decline in IGFBP 4 in N 3652 CM . [ Leu 27 ] IGF 2 was as potent as IGF 2 or IGF 1 in inducing changes in IGFBP 3 and IGFBP 4 concentrations . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Proliferation and differentiation of a human colon cancer cell line ( CaCo 2 ) is associated with significant changes in the expression and secretion of insulin like growth factor ( IGF ) IGF 2 and IGF binding protein 4 : role of IGF 2 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| This effect was completely inhibited in the presence of the IGF 2 antagonist , IGF binding protein 4 ( IGFBP 4 ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGF binding protein 4 ( IGFBP 4 ) counteracts the tumor promoting activities of IGF 2 by binding this growth factor . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To determine whether the effects of IGFs on IGFBP 4 might be exerted through alterations in IGFBP 4 degradation , we incubated cell free , fibroblast conditioned media from either sheep or human dermal fibroblasts with or without IGF 1 , IGF 2 ( each 1 microgram / ml ) , or insulin ( 10 micrograms / ml ) for 72 h at 37 C . ^^^ The demonstration that IGF 1 and IGF 2 can promote directly the proteolytic degradation of IGFBP 4 into fragments that do not bind IGFs provides a novel mechanism by which the IGFs may increase their own availability and / or activity in biological fluids . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Previous work indicated that hIGFBP 4 is the predominant IGFBP expressed by human osteoblast like cells , and that IGFBP 4 binds and inhibits the mitogenic activities of IGF 1 and IGF 2 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Differential effects of insulin like growth factor ( IGF ) 1 and IGF 2 on the expression of IGF binding proteins ( IGFBPs ) in a rat neuroblastoma cell line : isolation and characterization of two forms of IGFBP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Subsequent studies on the purification and characterization of IGFBP from DBcAMP treated TE 85 cell conditioned medium revealed that the IGFBP produced by TE 85 cells in response to DBcAMP treatment was identical to that of 25 kDa inhibitory IGFBP ( now designated as IGFBP 4 ) purified from TE 89 human bone cells based on : 1 ) N terminal amino acid sequence , 2 ) amino acid composition , 3 ) molecular weight and 4 ) inhibitory actions on basal and IGF 2 induced bone cell proliferation . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Proteolysis of the 29 kilodalton ( kDa ) [ 125I ] IGFBP 5 produced an 18 to 20 kDa fragment of IGFBP 5 , and 25 kDa [ 125I ] IGFBP 4 yielded two lower mol wt fragments in the presence of IGF 2 . ^^^ In contrast , CM from IGF 2 treated HBC cultures had increased proteolytic activity against IGFBP 4 . ^^^ To determine the mechanisms by which IGF 2 modulates IGFBP 4 and 5 proteolytic activity , CM from control U2OS cell culture was incubated with [ 125I ] IGFBP 4 or 5 in the presence of various concentrations of IGF 2 and IGF analogs under cell free conditions . ^^^ It was found that exogenous IGF 2 stimulated IGFBP 4 proteolysis , but IGF analogs that had no or extremely low affinity to IGFBP 4 failed to induce IGFBP 4 proteolysis . ^^^ Based on the findings that HBCs in culture produce a protease ( s ) capable of cleaving both IGFBP 4 and IGFBP 5 and that IGF 2 can promote or inhibit proteolytic degradation of IGFBP 4 and IGFBP 5 , respectively , it is proposed that IGFBP protease ( s ) may be an important modulator of IGF activity in bone . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGF 1 and IGF 2 but not insulin evoked a marked decrease of IGFBP 4 as early as 4 hours after treatment . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Serum levels of IGFBP 3 and IGFBP 4 tended to be higher in phosphoenolpyruvate carboxykinase IGF 2 transgenic mice than in controls , as evaluated by ligand blot analysis . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| The basal growth of the S P and C P cells was significantly increased in the presence of IGFBP 4 antibody , once again suggesting that endogenous IGFBP 4 was a potent inhibitor of autocrine effects of endogenous factors ( IGF 2 ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGF 2 activated IGFBP 4 hydrolysis was assessed by Western ligand blotting and quantitated using laser densitometry . ^^^ In comparison , hOB CM from cells treated with transforming growth factor beta ( TGF beta ) exhibited significantly augmented IGF 2 dependent proteolysis of endogenous IGFBP 4 . ^^^ Enhanced proteolysis of exogenous IGFBP 4 was also demonstrated : 1 ) 92 % of recombinant human IGFBP 4 added to conditioned medium from TGF beta treated hOB cells was hydrolyzed during the assay in the presence of IGF 2 compared to 45 % of recombinant human IGFBP 4 added to control hOB CM ; and 2 ) increased radiolabeled IGFBP 4 fragments were generated in conditioned medium from TGF beta treated hOB cells compared with control hOB CM in the presence of IGF 2 . ^^^ Our results indicate that the IGFBP 4 IGFBP 4 protease system in hOB cells can be controlled by two of the most abundant local growth factors for bone ( IGF 2 and TGF beta ) , with each acting via different mechanisms . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Our data indicate that elevated endogenous levels of IGF can activate IGFBP 4 proteolysis , because in hOB cultures lacking detectable IGFBP 4 protein 1 ) basal IGF messenger ribonucleic acid expression was increased ; 2 ) IGF 2 peptide levels were elevated ; 3 ) IGF neutralizing antibodies added to hOB CM attenuated the proteolysis of exogenous IGFBP 4 ; and 4 ) recombinant human IGFBP 4 was proteolyzed into 2 immunoreactive fragments of approximately 18 and 14 kilodaltons during cell free incubations in these hOB CM without the addition of exogenous IGF . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Both IGF 1 and IGF 2 were capable of inducing a decrease in IGFBP 4 ; however , IGF 2 was more effective . ^^^ When the six characterized IGFBP were added to hOB CM , only IGFBP 4 disappeared in response to IGF 2 addition . ^^^ To assess the biologic consequences of IGF 2 induced IGFBP 4 proteolysis , we treated hOB with IGF 2 for 5 h , which decreased medium IGF BP 4 by 70 % , and then measured IGF 1 and insulin stimulation of [ 3H ] thymidine incorporation . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Although the proteolytically modified IGFBP 4 retained the ability to bind IGFs , the affinities were approximately 13 and 20 fold lower for IGF 1 and IGF 2 , respectively . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Minimal activity ( e . g . < 20 % of IGFBP 4 degraded in 24 h at 37 C ) was present in conditioned medium unless IGF 1 or IGF 2 was added . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| A 24 h treatment with bFGF at 10 ( 8 ) M decreased IGF 1 mRNA by 97 % , IGF 2 mRNA by 73 % , IGFBP 2 by 64 % , IGFBP 4 by 73 % , IGFBP 5 by 95 % , and IGFBP 6 by 65 % . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Western ligand blot analysis of conditioned medium showed that treatment of stromal cells with IGF 1 or IGF 2 induced a dose dependent reduction of detectable IGFBP 4 levels . ^^^ In contrast , IGFBP 4 was undetectable in cultures receiving Leu 27 IGF 2 , which has reduced affinity for the type IIGF receptor but unaltered affinity for IGFBPs and the type 2 receptor . ^^^ The addition of IGF 1 or IGF 2 to cell free conditioned medium from stromal cells cultured in the absence of IGFs resulted in the reduction of detectable endogenous IGFBP 4 levels . ^^^ Cell free conditioned medium from endometrial stromal cultures proteolyzed covalently cross linked [ 125I ] IGF 2 IGFBP 4 complexes in the absence of added IGFs , generating an 18 kDa radiolabeled fragment , and addition of free IGF peptide did not enhance the degradation of IGF 2 IGFBP 4 complexes . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Upon formation of the S shape nephron , IGFBP 2 mRNA was expressed in the committed glomerular and epithelial cells which also expressed IGF 2 and IGFBP 4 , and the mesenchyme of the vascular cleft expressed IGFBP 5 mRNA . ^^^ In the maturing glomerulus , the glomerular epithelial cells expressed IGF 2 mRNA together with IGFBP 2 and IGFBP 4 mRNAs , while IGFBP 5 mRNA was localized to the mesangium and supporting mesenchyme . ^^^ The renal mesenchyme in the cortex and medulla expressed abundant IGF 2 mRNA , and lower levels of IGFBP 4 and 5 mRNAs . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| A 5 6 h incubation in human fibroblast conditioned medium in the presence of IGF 2 was sufficient for near total hydrolysis of wild type IGFBP 4 , whereas the mutant IGFBPs 4 were only minimally affected at this time . ^^^ When added with IGF 2 , the protease resistant mutant IG FBPs 4 , but not wild type IGFBP 4 , suppressed IGF 2 enhancement of IGF 1 stimulated DNA synthesis . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In contrast to the rapid and dramatic proteolysis in hOB medium , medium conditioned by these cells had no apparent IGFBP 4 protease activity when assayed with exogenous IGF 2 in culture or under cell free conditions . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 4 ( IGFBP 4 ) is a 24 kDa protein that binds insulin like growth factor 1 ( IGF 1 ) and IGF 2 with high affinity and inhibits IGF action in vitro . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Studies on the molecular mechanisms for IGFBP 4 actions revealed that coincubation of bone cells with IGFBP 4 and 125I IGF 1 or 125I IGF 2 decreased the binding of both of these ligands in a dose dependent manner . ^^^ These data in conjunction with data showing that IGFBP 4 had no effect on cell proliferation induced by analogs of IGF 1 or IGF 2 , which exhibited > 100 fold reduced affinity for binding to IGFBP 4 suggest that IGFBP 4 may inhibit IGF action by preventing the binding of ligand to its membrane receptor . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In this study , we describe the mRNA expression of IGF 1 , IGF 2 , type 1 IGF receptor , IGFBP 2 , IGFBP 4 and IGFBP 5 during mouse lung development as studied by in situ hybridization techniques . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| On the other hand , IGF 2 treatment resulted in a significant decrease in the 24 kDa IGFBP 4 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To determine if growth factors produced by MC3T3 E 1 cells have autocrine actions on these cells , the effects of IGF 1 , IGF 2 , TGF beta 1 , and IGFBP 4 on MC3T3 E 1 cell proliferation were determined . ^^^ Exogenous addition of IGF 1 and IGF 2 stimulated MC3T3 E 1 cell proliferation , but TGF beta 1 and IGFBP 4 inhibited MC3T3 E 1 cell proliferation . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Treatment of normal adult human fibroblasts with 10 nM insulin like growth factor 2 ( IGF 2 ) for 24 h resulted in an 85 % decrease in endogenous IGFBP 4 , as assessed by Western ligand blot analysis of the conditioned medium . ^^^ Incubation of human fibroblast conditioned medium ( HFCM ) with IGF 2 under cell free conditions led to a similar loss of IGFBP 4 . ^^^ IGF 2 was the most effective of the natural and mutant IGFs tested , inducing complete hydrolysis of rhIGFBP 4 at a molar ratio of 0 . 25 : 1 ( IGF / IGFBP 4 ) . ^^^ In conclusion , these data provide evidence for a novel IGF dependent IGFBP 4 specific protease that modifies IGFBP 4 structure and function , and indicate a preferential role for IGF 2 in its activation . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Pelvic leaflets were incubated in serum free medium for 18 h with effectors ( BSA , IGF 1 , IGF 2 , IGFBP 4 , or a combination of IGF and IGFBP 4 ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Messenger RNAs for IGF 1 , IGF 2 , and IGFBP 4 were detected by Northern analysis . ^^^ AGS cells expressed messenger ( mRNA ) RNAs for IGF 2 and IGFBP 4 but not for IGF 1 , whereas another gastric carcinoma cell line ( SIIA ) , whose growth is stimulated by IGF 1 , expressed mRNA IGF 2 but did not express mRNA for IGF 1 or IGFBP 4 : CONCLUSIONS : The relative absence of growth response of AGS cells to IGF 1 is due to the endogenously produced IGFBPs sequestering IGF 1 and preventing receptor interaction . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| We report that the basal expression of IGF 2 , IGF binding protein ( IGFBP ) 3 , and IGFBP 4 was higher in LSaOS cells than in HSaOS cells with the opposite true for type 1 IGF receptor . ^^^ DBcAMP treatment of LSaOS cells decreased IGF 2 and IGFBP 3 but increased IGFBP 4 and type 1 IGF receptor ; no effect was observed for the type 2 IGF receptors . ^^^ DBcAMP treatment of HSaOS cells had no detectable effect on IGF 2 ; IGFBP 3 , or type 1 and type 2 IGF receptor expression ; only IGFBP 4 expression increased with DBcAMP . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| The IGFBP 4 protease assay involved incubation of 50 microliters HFCM with or without 5 nM IGF 2 for 6 h at 37 C under cell free conditions ; IGF activated IGFBP 4 hydrolysis was assessed by Western ligand blotting . ^^^ In HFCM from cells treated with vehicle , GH , insulin , epidermal growth factor , steroid hormones , or forskolin , IGF 2 induced the select loss of detectable IGFBP 4 during the assay . ^^^ In contrast , IGFBP 4 levels were maintained when HFCM from cells treated with phorbol ester tumor promoters was incubated with IGF 2 under cell free conditions . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Enhanced cell responsiveness to IGF 1 appears to be due to IGF 2 induced changes in pericellular IGFBP 3 and IGFBP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 4 ( IGFBP 4 ) , like the five other IGFBPs present in human serum , acts as a transport protein for insulin like growth factor 1 ( IGF 1 ) and IGF 2 and modulates their biological effects . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In this study , SK N SH neuroblastoma cells were shown to produce IGF 2 , IGFBP 2 , IGFBP 4 and small quantities of IGFBP 6 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGFBP 4 levels were diminished by increasing concentrations of IGFs in the CM of the NSCLCs A 549 , NCI H 157 , and U 1752 , with no response to insulin or the IGF 1 analog , whereas IGFBP 4 specific mRNA was not changed by IGF 1 or IGF 2 , as determined by Northern analysis . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with IGF 1 and IGF 2 resulted in a dose dependent increase of IGFBP 5 and a small increase in IGFBP 4 in conditioned media ( CM ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Chondrocytes from osteoarthritic cartilage have increased expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) and 5 , but not IGF 2 or IGFBP 4 . ^^^ Message levels for beta actin , IGF 2 , and IGFBP 4 were unchanged between the cartilage types . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| We investigated the expression of the mRNAs encoding IGF 1 , IGF 2 , the type 1 IGF receptor ( IGF T1R ) and two IGF binding proteins ( IGFBP 2 and IGFBP 4 ) in rat lung during the perinatum . ^^^ At day 20 of gestation IGF 1 , IGF T1R and IGFBP 4 mRNA levels were higher in lung than liver ( all P < 0 . 01 ) , whereas IGF 2 and IGFBP 2 mRNA levels were higher in liver than lung ( each P < 0 . 02 ) . ^^^ The patterns of expression of IGF 1 , IGF T1R , IGFBP 2 and IGFBP 4 , but not IGF 2 , in developing lung correspond to previously described phasic changes in lung cell proliferation rates . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGFBP 2 and IGFBP 4 in human intestinal epithelial cells : is it a way of modulating IGF 2 bioavailability towards the IGF responsive basolateral surface . ^^^ In early differentiated cells , i . e . , up to day 7 post confluence , IGF 2 , IGFBP 2 , IGFBP 4 ( > 96 % ) and IGFBP 6 ( approximately 85 % ) are recovered in the basolateral medium . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Recent studies demonstrate that IGF 2 in FFe is higher than in FFa ' whereas IGF 1 , IGFBP 3 and IGFBP 1 levels do not differ , underscoring the importance of local IGF 2 production by the granulosa and the importance of IGFBP 4 and IGFBP 2 in regulation of IGF 2 action within the follicle during its developmental pathway as an E 2 or A dominant follicle . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGFBP 4 proteolytic activity was stimulated by IGF 1 or IGF 2 added exogenously and was inhibited by EDTA or protease inhibitors . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Increased serum had no effect on levels of IGF 2 mRNA , IGFIR , IGFBP 4 mRNA , or IGFBP secreted in MI 7 cells . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Under basal conditions , neuroblastoma cells secrete IGFs ( essentially IGF 2 ) , IGFBPs ( IGFBP 4 and predominantly IGFBP 2 that is partially proteolysed ) , and proteases , including tissue type plasminogen ( PLG ) activator , whose activity is inhibited by PLG activator inhibitor 1 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Under basal conditions , SH SY5Y cells in serum free medium secreted IGF 2 , and traces of IGF 1 , IGFBP 2 and IGFBP 4 . ^^^ After 48 h , when IGFBP 2 and IGFBP 4 concentrations in the culture media had increased , des ( 1 3 ) IGF 1 remained the most active , but the activity of insulin now equalled or exceeded that of IGF 1 and IGF 2 . ^^^ This suggests a negative feedback mechanism involving partial sequestration of IGF 1 and IGF 2 by IGFBP 2 and IGFBP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Immunostaining of sections of normal and cancer tissue was negative for IGF 1 and IGF 2 ; IGFBP 2 was positive in 2 out of 10 cancer tissues and 7 out of 10 normal tissues ; IGFBP 3 was positive in 7 out of 10 cancer tissues and 7 out of 10 normal tissues ; and IGFBP 4 was positive in 5 out of 10 cancer tissues and 6 out of 10 normal tissues . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To determine insulin like growth factor ( IGF ) 1 and IGF 2 levels , IGF binding protein ( IGFBP ) profile , and IGFBP 4 protease activity in androgen dominant follicular fluid ( FF ) from female to male trans sexuals and to compare with those in follicles from normocycling women . ^^^ CONCLUSION ( S ) : Follicles developing under the influence of exogenous androgens in ovaries in female to male trans sexuals appear to be similar to androgen dominant follicles in normo ovulatory women with regard to IGF 1 and IGF 2 levels , IGFBP profile , and the absence of IGFBP 4 protease activity . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| These IGFBPs , except the 24 kd form ( IGFBP 4 ) , were modulated by both IGF 1 and IGF 2 , with a maximum effect at 100 and 10 nmol / L , respectively . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that secretion of IGFBP 4 and IGFBP 5 by L 6 and BC3H 1 muscle cells was stimulated by treatment with either insulin , IGF 1 , or IGF 2 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Elevated insulin like growth factor ( IGF ) binding protein ( IGFBP ) 2 and IGFBP 4 expression of leukemic T cells is affected by autocrine / paracrine IGF 2 action but not by IGF type 1 receptor expression . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Mitogenic studies revealed that an IGFBP 4 mutant ( His 74 replaced by Pro 74 ) and an N terminal peptide ( N terminus to Thr 71 ) with little IGF binding activity failed to inhibit IGF 2 induced human osteoblast proliferation . ^^^ In contrast , an IGFBP 4 mutant ( His 74 replaced with Ala 74 ) exhibited similar IGF binding activity and potency in inhibiting the activity of IGF 2 compared with the wild type . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| As has been shown for other cell types , we found that IGFBP 4 had only inhibitory actions , inhibiting IGF 1 and IGF 2 stimulated proliferation and differentiation . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| The injured sciatic nerve expressed IGF 1 and IGF 2 as well as IGFBP 4 and IGFBP 5 , whereas central nervous system ( CNS ) scar tissue expressed IGF 1 , IGFBP 2 , and IGFBP 5 . ^^^ In line with the in vivo findings , cultured Schwann cells expressed IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 5 mRNAs , whereas cultured astrocytes expressed IGF 1 , IGFBP 2 , and IGFBP 5 mRNAs . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In AN , the levels of GH binding protein , free and total IGF 1 , free IGF 2 , and IGFBP 3 were significantly reduced ; total IGF 2 , IGFBP 2 , and IGFBP 4 levels were unchanged ; and IGFBP 1 was increased . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Surface bound plasmin induces selective proteolysis of insulin like growth factor ( IGF ) binding protein 4 ( IGFBP 4 ) and promotes autocrine IGF 2 bio availability in human colon carcinoma cells . ^^^ HT 29 D4 cells secreted IGF 2 totally complexed to IGFBP 2 , IGFBP 4 and IGFBP 6 . ^^^ IGFBP 4 proteolysis yielded 18 and 14 kDa immunoreactive fragments which were not detectable by Western ligand blotting , indicating that they bound IGF 2 with poor affinity . ^^^ Release of IGF 2 from IGF 2 IGFBP complexes after IGFBP 4 proteolysis by cell bound Pm was indicated by the observation that approximately 20 % of the 125I IGF 2 initially associated with endogenous IGFBP in reconstituted complexes was transferred to HT 29 D4 cell surface IGF 1 receptors . ^^^ These results suggest that IGFBP 4 proteolysis by cell bound Pm can promote autocrine / paracrine IGF 2 bio availability in colon cancer cells . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Studies on the role of human insulin like growth factor 2 ( IGF 2 ) dependent IGF binding protein ( hIGFBP ) 4 protease in human osteoblasts using protease resistant IGFBP 4 analogs . ^^^ To characterize the insulin like growth factor binding protein 4 ( IGFBP 4 ) protease produced by human osteoblasts ( hOBs ) , we localized and determined the role of the proteolytic domains in human IGFBP 4 ( hIGFBP 4 ) in modulating IGF 2 actions . ^^^ N terminal amino acid sequence and mass spectrometric analyses of the 6xHis tagged IGFBP 4 proteolytic fragments revealed that Met 135 Lys136 was the only cleavage site recognized by the IGF 2 dependent IGFBP 4 protease produced by hOBs . ^^^ To determine the physiological significance of the IGF 2 dependent IGFBP 4 protease , we compared the effect of the wild type IGFBP 4 and the protease resistant IGFBP 4 analogs in blocking IGF 2 induced cell proliferation in normal hOBs , which produce IGFBP 4 protease , and MG 63 cells , which do not produce IGFBP 4 protease . ^^^ It was found that protease resistant IGFBP 4 analogs were more potent than the wild type protein in inhibiting IGF 2 induced cell proliferation in hOBs but not in MG 63 cells . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Circulating concentrations of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 , IGFBP 5 , 25 hydroxycholecalciferol ( 25 ( OH ) D 3 ) , and intact parathyroid hormone ( PTH ) were measured in 50 elderly women after sustaining a hip fracture and in 50 healthy age matched controls . ^^^ No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : GH significantly increased serum IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 4 and decreased serum IGFBP 1 and IGFBP 2 in post menopausal women . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGF 1 or IGF 2 ( 6 . 5 nM ) preferentially induced the accumulation of IGFBP 3 ( 1 . 9 fold ) and to a lesser extent of IGFBP 4 , but did not show any effect on IGFBP 1 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGF 2 mRNA was detected in trophoblasts invading the walls of maternal vessels , and the endothelium of the preplacental vessels expressed IGFBP 4 mRNA , while IGFBP 2 and IGFBP 5 mRNAs were present in the tunica media of mesometrial arteries that had not been invaded by trophoblast . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| After stable transfection with N myc cDNA , Northern blotting revealed a marked increased in IGF IR , IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 4 mRNA levels , whereas IGFBP 6 mRNA levels were clearly diminished . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor ( IGF ) binding protein 4 ( IGFBP 4 ) , a consistent inhibitor of IGF action , is subject to proteolytic cleavage by the IGF 2 dependent IGFBP 4 protease . ^^^ However , regulation of the IGF 2 dependent IGFBP 4 protease in vivo is not known . ^^^ Results from in vitro protease assays using recombinant IGFBP 4 revealed that IGFBP 4 proteolysis determined in both the presence and absence of exogenous IGF 2 significantly increased during the first and second trimesters and reached a plateau by the third trimester . ^^^ However , in the absence of IGF 2 , IGFBP 4 proteolysis by pregnancy serum was only observed after prolonged incubation . ^^^ IGF 2 dose dependently increased IGFBP 4 proteolysis by pregnancy serum , with maximal stimulation observed at a concentration of 0 . 7 mol / L relative to IGFBP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To test the hypothesis that impairment in bone formation in renal osteodystrophy in adults with chronic renal failure ( CRF ) might be mediated in part by alterations in circulating levels of the insulin like growth factor ( IGF ) system components , we compared serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 3 , IGFBP 4 and IGFBP 5 in adults with CRF ( CRF patients with parathyroid hormone ( PTH ) < 100 pg / ml , PTH > 300 pg / ml and end stage renal failure ( ESRF ) patients ) versus age matched controls . ^^^ We found severalfold increased serum levels of IGFBP 3 ( 2 fold ) , IGFBP 4 ( 5 fold ) and slightly increased IGF 2 levels in ESRF patients as well as a 2 . 6 fold increase in free IGF 1 in CRF patients with PTH < 100 pg / ml . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| However , IGF 2 levels , IGFBP 4 profiles , and IGFBP 3 protease activity did not differ significantly between the two groups . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Plasmon resonance spectroscopy , ligand blot analysis , and saturation and displacement studies demonstrated a very low affinity of the C terminal IGFBP 4 fragment for the IGFs ( IGF 2 , K ( d ) = 690 nM ; IGF 1 , K ( d ) > 60 nM ) , whereas the N terminal fragment retained significant IGF binding properties ( IGF 2 , K ( d ) = 17 nM ; IGF 1 , K ( d ) = 5 nM ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Evidence that the interaction between insulin like growth factor ( IGF ) 2 and IGF binding protein ( IGFBP ) 4 is essential for the action of the IGF 2 dependent IGFBP 4 protease . ^^^ A variety of human cell types , including human osteoblasts ( hOBs ) , produce an IGFBP 4 protease , which cleaves IGFBP 4 in the presence of IGF 2 . ^^^ Recently , the pregnancy associated plasma protein ( PAPP ) A has been determined to be the IGF 2 dependent IGFBP 4 protease produced by human fibroblasts . ^^^ This study sought to define the mechanism by which IGF 2 enhances IGFBP 4 proteolysis . ^^^ Pre incubation of IGFBP 4 with IGF 2 , followed by removal of unbound IGF 2 , led to IGFBP 4 proteolysis without further requirement of the presence of IGF 2 in the reaction . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| GH treatment normalized serum IGF 1 in a majority of the patients and increased IGFBP 3 and 5 as well as IGFBP 4 and IGF 2 to values within normal range . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To eliminate possible actions of IGF 2 , if any was produced by osteoblasts derived from IGF 1 knockout mice , the IGFBP 5 effect was studied in the presence of exogenously added IGFBP 4 , a potent inhibitor of IGF 2 actions in bone cells . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| No influence of T ( 3 ) on IGF 1 , IGF 2 , IGFBP 3 , or IGFBP 4 mRNA levels in hOB was observed . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGFBP 4 proteolysis was inhibited by EDTA and 1 , 10 phenanthroline and was accentuated by the addition of IGF 1 and IGF 2 and , to a lesser extent , by des ( 1 3 ) IGF 1 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| The concentrations of IGFBP 2 and IGFBP 4 decreased by 48 + / 6 and 70 + / 13 % , respectively , whereas that of IGFBP 6 increased by 698 + / 20 % with 1 microM tRA . tRA decreased mRNA levels of IGFBP 2 and IGFBP 4 by 20 + / 3 and 50 + / 8 % , respectively , whereas tRA increased IGFBP 6 mRNA by 660 + / 20 % . tRA did not alter levels of IGF 2 mRNA or peptide . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Thirty two tumors were positive for IGF 1 , 39 for IGF 2 , 48 for IGF IR , and 35 for IGFBP 4 mRNA . ^^^ Seventeen tumors were concomitantly positive for all four IGFs , whereas 34 were positive for IGF 2 , IGF IR , and IGFBP 4 mRNA . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Insulin like growth factor binding protein 4 ( IGFBP 4 ) is , like the other five IGFBPs , a critical regulator of the activity of insulin like growth factor ( IGF ) 1 and IGF 2 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| HT 29 cells treated with retinoic acid had dose dependent increases in IGFBP 4 and reduced IGF 2 expression . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Levels of IGFBP 2 , IGFBP 4 , IGF 1 and IGF 2 in media conditioned by 75 day fetal S 5 cultures were not influenced by late DEX . ^^^ Early DEX treatment increased ( P < 0 . 05 ) IGFBP 4 levels in 75 day S 5 media but had little to no influence on levels of IGF 1 , IGF 2 and other IGFBPs . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Addition of IGF 2 to mPS or mNPS at a dose sufficient to increase IGFBP 4 proteolysis by human PS failed to enhance IGFBP 4 proteolysis . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Presumed functions at the maternal fetal interface are to proteolyze IGFBP 4 and thus increase IGF bioavailability locally in the placenta , to promote IGF 2 mediated trophoblast invasion into the maternal decidua , and to modulate IGF regulation of steroidogenesis and glucose and amino acid transport in the villous . ^^^ Together , these data demonstrate the presence of an IGF 2 and metal dependent IGFBP 4 protease activity in human trophoblast plasma membranes , identified as PAPP A , which is well situated to proteolyze IGFBP 4 at the maternal placental interface to facilitate IGF action at the villous surface and / or the invading extravillous cytotrophoblast . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| As reported for native IGFBPs , IGF 2 bound with affinity higher than IGF 1 to recombinant IGFBP 3 and IGFBP 4 ( K ( D ) of < 0 . 05 nmol ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Although they appeared as cellular clusters with a uniform epithelial phenotype , immunohistochemical analyses of components of the IGF system revealed an unique pattern : weak immunoreactivity could be seen for IGF 2 while among all IGF binding proteins ( IGFBPs ) only IGFBP 6 and weakly IGFBP 4 were detectable in epithelial cells of Malassez . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Ovarian IGFBP 4 mRNA levels were markedly increased after treatment with the LH analog , hCG , whereas IGF 2 and PAPP A mRNAs were not significantly altered . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In normal testes a clear expression of IGF 1 , IGF 2 , IGF IR , IGFBP 2 , IGFBP 4 and IGFBP 5 was found . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^ When VA deficient rats received a single injection of retinoic acid ( 2 mg / rat ) , tissue IGF 1 and IGF IR gene expression did not change after 4 or 8 h , while the expression of IGF 2 , IGFBP 4 , and IGFBP 6 mRNAs in some tissues increased rapidly . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| However , IGFBP 4 proteolysis in cell free assay was dependent on IGF 2 , and there was increased IGF 2 dependent IGFBP 4 protease activity in conditioned medium from hOB cells that were treated with TGF beta . ^^^ IGF 1 stimulation of hOB cell proliferation was markedly enhanced by pretreatment with TGF beta and [ Leu 27 ] IGF 2 , and this enhancement was prevented with protease resistant IGFBP 4 . ^^^ However , functional activation of the IGFBP 4 protease system is dependent on IGF 2 , which acts at a post translational level . ^^^ These data support a model whereby local TGF beta and IGF 2 in the bone microenvironment coordinately amplify IGF 1 bioavailability through controlled IGFBP 4 proteolysis , which may be a means to promote bone formation . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In contrast , levels and / or sites of expression of IGF 1 , IGF 2 , IGFBP 4 , IGFBP 5 and type 2 receptor in follicular cells strongly differ between mammalian species , suggesting that these phenomena might play species specific or secondary roles in ovarian folliculogenesis . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGFBP 4 binds IGF 1 and IGF 2 with similar affinities and inhibits their actions under almost all in vitro and in vivo conditions . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In the 26 predictor genes , we found alteration of the expression of genes regulating cell division ( NAP1L1 , STMN 1 , CCNG 2 , and CDC7L1 ) and tumor cell growth ( IGF 2 and IGFBP 4 ) in HBT . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Ligand blot analysis of conditioned medium using ( 125 ) 1 IGF 2 revealed that the production of IGFBP 2 and IGFBP 4 was also decreased by t10c12 CLA , whereas c9t11 CLA had no effect . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Soluble IGFBP 5 and IGFBP 4 depressed the binding of ( 125 ) 1 IGF 1 and ( 125 ) 1 IGF 2 to the IGF 1R , but did not affect binding of ( 125 ) 1 R ( 3 ) IGF 1 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| PAPPA , IGF 2 and IGFBP 4 transcripts co localized in wild type embryos , and expression of IGF 2 and IGFBP 4 mRNA was not altered in PAPPA deficient embryos . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Degradation of IGFBP 4 by PAPP A or MC3T3 E 1 conditioned medium was enhanced by IGF 2 but inhibited by mutation of basic residues at or near the PAPP A cleavage site in IGFBP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Ligand blotting revealed that both IGF 1 and 2 increased IGFBP 2 and 5 ( protein ) and had no effect on IGFBP 3 ( protein ) , whereas IGF 1 ( but not IGF 2 ) increased IGFBP 4 ( protein ) , suggesting IGFBP 2 , 4 , and 5 are post transcriptionally regulated . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Results showed that ES induced a change in phenotype that was accompanied by downregulation of igf 2 and upregulation of igfbp 4 mRNA levels . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGFBP 3 , IGFBP 4 and PAPP A mRNA expression and IGF 2 concentration did not differ ( P > 0 . 10 ) between dominant or subordinate follicles . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Furthermore , an IGFBP 3 protease cleaving also IGFBP 2 but not IGFBP 4 was co purified from the IGF 2 column . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| IGF 2 , IR B , IGFBP 1 , IGFBP 4 , and IGFBP 6 mRNA were downregulated in seminoma . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Effects of hormone replacement therapy on insulin like growth factor ( IGF ) 1 , IGF 2 and IGF binding protein ( IGFBP ) 1 to IGFBP 4 : implications for cardiovascular risk . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| There was no correlation of plasma IGFBP 4 level with age , sex , immunophenotype , or ALL risk group , and there was no correlation of IGFBP 4 level with plasma IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 levels . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Microarray analysis of implantation and explantation samples of recovery patients further revealed elevated IGF 2 and IGF binding proteins IGFBP 4 and IGFBP 6 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In the yolk sac , IGF 2 mRNA was expressed in endoderm and mesoderm , whereas IGFBP 1 , 2 and 4 mRNAs were expressed only in endoderm , and IGFBP 4 mRNA in mesoderm . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Also , parous mammary glands before MNU treatment exhibited down regulation of growth related genes such as regenerating islet derived 3 alpha ( Reg3a ) , mesothelin ( Msln ) , insulin like growth factor 2 ( Igf 2 ) and insulin like growth factor binding protein 4 ( Igfbp 4 ) . ^^^ After MNU treatment , AMV mammary glands exhibited up regulation of growth related genes , such as Msln , cell division cycle 2 homolog A ( Cdc2a ) , Igf 2 , Igfbp 4 , stathmin 1 ( Stmn 1 ) and homeobox , msh like 1 ( Msx 1 ) , whereas expression of these genes remained low in parous mammary glands . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In fast muscle , there was a decrease in IGF 2 ( P < 0 . 01 ) on embryonic day ( ED ) 16 and an increase in IGFBP 2 ( P < 0 . 01 ) and IGFBP 4 ( P < 0 . 05 ) and in IGFBP 5 ( P < 0 . 05 ) expression on ED 18 in the stimulated group compared with the control group . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Comparison of follicular fluid IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and PAPP A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF embryo transfer patients . ^^^ This is the first study that has compared follicular fluid ( FF ) IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and pregnancy associated plasma protein ( PAPP ) A concentrations , and their ratios , to investigate whether there was any difference in the intrafollicular microenvironment between the GnRH agonist ( GnRHa ) and antagonist ( GnRHant ) protocols for controlled ovarian stimulation ( COS ) . ^^^ IGF 1 , IGF 2 and IGFBP 3 concentrations were measured by radioimmunoassay and IGFBP 4 and PAPP A by enzyme linked immunosorbent assay . ^^^ The concentrations of FF IGF 2 and IGFBP 4 were significantly higher , and the ratio of IGF I / IGFBP 4 was significantly lower in the GnRHa long protocol group . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| In addition , plasma GH profiles and plasma concentrations of IGF 1 , IGF 2 , and insulin like growth factor binding protein 4 and 6 ( IGFBP 4 and 6 ) were measured to assess their potential systemic role during bone formation . ^^^ Plasma concentrations of GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 did not demonstrate any significant differences between treatment groups and controls . ^^^ Changes in the systemic osteotropic growth factors GH , IGF 1 , IGF 2 , IGFBP 4 , and IGFBP 6 do not seem to be of importance during distraction osteogenesis . . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| To determine whether IGFs may participate in the initiation of restorative reactions in inactivated muscle we analyzed the expression of IGF 1 and IGF 2 mRNA in botulinum toxin paralyzed and in denervated rat skeletal muscle , and interfered with the activity of IGFs by locally applying the IGF binding proteins IGF BP 5 and IGF BP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| These differences may account for the preferential affinity of BP 4 for IGF 2 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| Serum concentrations of IGF 1 , IGF 2 , BP 1 , and BP 3 were determined by immunoradiometric assays , and Western ligand blots provided a semiquantitative measurement of BP 2 , BP 3 , and BP 4 . ^^^ |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22692 and P01344 |
Pubmed |
SVM Score :0.0 |
| NA |
|