Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
NA
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
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Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.67751555
IGF 2 was positively correlated with both IGFBP 3 and IGFBP 1 . 0.67751555^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.56904696
Serum levels of IGF 1 and especially IGF 2 and their major growth hormone dependent binding protein ( IGFBP 3 ) were significantly reduced , although growth hormone secretion after a pharmacological stimulus was normal . 0.56904696^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This results in sequestration of IGFBP 3 and predominant association of big IGF 2 with IGFBP 2 and 3 in the 50 kD complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin , but not IGF 2 , mimicked IGF 1 ' s action on IGFBP 3 and 2 production , but was less potent . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The IGFBP 3 gene is coexpressed with the IGF 2 gene in proliferative cytotrophoblasts of the embryonic placenta . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Similar to IGF 1 , IGF 2 treatment increased cell number and resulted in the appearance of IGFBP 3 and the 29K IGFBP . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 2 and growth hormone dependent IGF binding protein 3 were also low . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Added IGF 2 lowered the amount of recombinant IGFBP 3 measured by the 5C11 / 7F8 assay , but not by the 1B6 / 5C11 assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Circulating IGF 1 and IGF 2 were measured by RIA , and IGFBP 3 was measured by both RIA and immunoradiometric assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The IGF 1 , IGF 2 , and IGFBP 3 levels measured by RIA were 18 % , 14 % , and 16 % of those in serum , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Neutralizing antibodies directed against either IGF 1 or IGF 2 or IGF binding protein 3 blocked protein accumulation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Nocturnal levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 remained stable during both studies . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma immunoreactive IGF 1 and IGF 2 , and serum IGFBP 1 levels were normal , but serum levels of IGFBP 3 were increased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Enhanced cell responsiveness to IGF 1 appears to be due to IGF 2 induced changes in pericellular IGFBP 3 and IGFBP 4 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 rose , IGF 2 decreased , and IGFBP 3 remained constant . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Values of IGF 1 , IGF 2 , and IGF BP 3 were low . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 and IGFBP 3 concentrations were low during the fetal life and increased after birth . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , oestradiol and norethisterone were analysed by radioimmuno assays . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the SGA fetus , IGF 2 was low ( P = 0 . 0009 ) and IGFBP 3 ( P = 0 . 02 ) was high . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
ALS in adult sera more tightly correlated with IGFBP 3 than IGF 1 or IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The increase in IGFBP 3 fragmentation correlated negatively with alterations in IGF 1 and IGF 2 ( P < 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
By contrast , Dex decreased IGFBP 3 levels , the latter increasing the bioavailability of IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Liver RNA was analyzed for mRNAs specific for the GH receptor , IGF 1 , IGF 2 , and IGF binding protein 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Furthermore , we measured serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in the patients . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Exogenous IGFBP 3 , 4 , and 5 had an inhibitory action on IGF 2 dependent DNA synthesis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Subsequently , IGF 2 , IGFBP 2 and IGFBP 3 were determined in the HPLC fractions by specific RIAs . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Exposure to IGF 1 or IGF 2 ( 13 or 26 nM ) caused an increase in expression and release of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in plasma were measured by use of immunoassay kits . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 and IGF 2 were not different among the patient groups and controls ( P > . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The levels of IGF 1 , IGF 2 and IGFBP 3 were determined by specific radioimmunoassays ( RIAs ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) were measured by radioimmunoassay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No influence of T ( 3 ) on IGF 1 , IGF 2 , IGFBP 3 , or IGFBP 4 mRNA levels in hOB was observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In combination with Tf , IGFBP 3 showed a 5 fold higher affinity for IGF 2 than IGFBP 3 alone . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Laboratory investigations at the age of 19 months revealed high levels of IGF 1 , IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Measurements of IGF 1 , IGF 2 and IGF binding protein 3 concentrations were performed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 levels were measured by radioimmunoassay , and IGFBP 3 levels were measured by immunoradiometric assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and IGFBP 6 were examined by specific assays . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
For GH R , IGFR 1 , IGF 2 , IGFBP 3 and 4 mRNA expression , we found a significant down regulation in certain stages . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Endometrial mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 increased in bST C , but not in bST P cows . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 2 , IGFBP 2 , and IGFBP 3 levels did not change . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma samples of IGF 1 , IGF 2 , IGF binding protein 3 , and C peptide were collected before initiation of chemotherapy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Due to low growth hormone levels , hepatic production of IGFBP 3 ( the main binding protein of IGF 2 ) is impaired . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IVF children are taller with increased IGF 1 , IGF 2 and IGFBP 3 expression . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Before treatment , most patients had increased basal serum concentrations of growth hormone ( 2 . 4 208 mU / l ) and low serum concentrations of IGF 1 ( < 20 69 micrograms / l ) , IGF 2 ( 69 295 micrograms / l ) and IGF binding protein 3 ( 0 . 16 1 . 59 mg / l ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In this study , we sought to determine by Western ligand blotting the effects of growth hormone , IGF 1 and IGF 2 on the production of IGFBP 3 and IGFBP 4 in TE 89 human osteosarcoma cells and in untransformed normal human bone cells derived from rib . ^^^ IGF 1 and IGF 2 , which increased human bone cell proliferation , decreased the level of IGFBP 4 ( 30 % of control at 100 micrograms / l IGF 1 and IGF 2 ) but increased the level of IGFBP 3 ( 3 10 fold at 100 micrograms / l IGF 1 and IGF 2 ) after 48 h of treatment in the conditioned medium of both low and high density TE 89 cell cultures . ^^^ Similar changes in IGFBP 3 and IGFBP 4 levels were also seen in the conditioned medium of human bone cells derived from rib after treatment with IGF 1 and IGF 2 . ^^^ Studies to determine the underlying molecular mechanisms by which IGF 2 decreased the amount of IGFBP 4 in the conditioned medium revealed that IGF 2 decreased the IGFBP 4 mRNA abundance and increased the IGFBP 3 mRNA abundance in human bone cells . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGF binding proteins ( IGFBP 1 , IGFBP 3 ) , GH and insulin were measured by radioimmunoassay . ^^^ Mean levels of IGFBP 1 and IGFBP 3 , IGF 2 and GH were unaffected by IGF 1 administration . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF bioactivity , measured in a porcine cartilage bioassay , was 0 . 18 and 0 . 55 U / mL in subjects 1 and 2 ; differences in bioactivity between subjects did not relate to serum IGF 2 levels , but , rather , to differences in IGFBP 3 levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As determined by ligand blot of CM from confluent cells 72 h after the addition of peptides to serum free medium , IGF 1 and IGF 2 potently stimulated IGFBP 3 in both cell lines , but otherwise IGFBP regulation in the two cells diverged . ^^^ In N 3652 cells , IGFBP 3 concentrations in CM increased to 700 % and 800 % of basal levels in the presence of IGF 1 and IGF 2 ( at 100 ng / ml ; n = 5 experiments ) , respectively . ^^^ In SCL 1 cells , IGF 1 and IGF 2 were virtually identical in stimulating a mean 200 % increase in IGFBP 3 ( n = 5 experiments ) . ^^^ To test the hypothesis that IGF induced alterations in IGFBP 3 and IGFBP 4 concentrations were regulated via the type 1 IGF receptor , we attempted to block IGFBP changes with type 1 IGF receptor antibody alpha IR 3 and to induce IGFBP changes with an IGF 2 analog , [ Leu 27 ] IGF 2 , with little affinity for the type 1 receptor . alpha IR 3 failed to block either the IGF induced rise in IGFBP 3 in each cell line or the decline in IGFBP 4 in N 3652 CM . [ Leu 27 ] IGF 2 was as potent as IGF 2 or IGF 1 in inducing changes in IGFBP 3 and IGFBP 4 concentrations . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In contrast , normal lactating breast contains only IGFBP 2 and IGF 2 messages ; IGFBP 3 and 4 mRNAs are absent in this tissue . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To determine whether chronic hypoxemia results in alterations in endocrine function that may contribute to growth failure , we measured growth hormone ( GH ) , somatomedins ( insulin like growth factors 1 and 2 , IGF 1 and IGF 2 ) , hepatic growth hormone receptors , and circulating IGF binding proteins IGFBP 3 and IGFBP 2 in 12 newborn lambs with surgically created pulmonic stenosis and atrial septal defect , and in 10 controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We suggest that after GH treatment IGF 2 is sequestered by stimulated IGFBP 3 in association with a pre existing acid labile subunit to form high molecular weight complexes which prevent IGF 2 gaining access to tissues receptors through which it exerts its hypoglycaemic effects . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Treatment of MECs with either IGF 1 or IGF 2 increased the amount of both the 29K IGFBP and IGFBP 3 in the CM , with relative potencies similar to those of the lactogenic hormones . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The binding proteins IGFBP 1 and IGFBP 3 did not cross react , but both IGF 1 and IGF 2 markedly inhibited IGFBP 6 tracer binding to antiserum . ^^^ This inhibition , greater for IGF 2 than for IGF 1 , was fully reversed by the addition of IGFBP 3 to sequester the IGFs . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
When analyzed by SDS PAGE and [ 125I ] IGF 2 ligand blotting , human neonatal fibroblasts secrete IGFBP 3 , an IGFBP of 29 31 kDa , and a 22 24 kDa IGFBP after treatment with 50 ng / ml IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied the relationship of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , the IGF binding proteins IGFBP 1 , IGFBP 2 , and IGFBP 3 , and GH binding protein ( GHBP ; which is postulated to be derived from the extracellular portion of the GH receptor ) in normal volunteers and patients with anorexia nervosa before and after a refeeding program . ^^^ Both serum IGF 1 and IGF 2 were positively correlated with serum IGFBP 3 and negatively correlated with serum IGFBP 1 and IGFBP 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The percent inhibition was not significantly increased when the concentration of serum was decreased to 0 . 2 % , which induced 140 % stimulation of DNA synthesis ; 2 ) stimulation by 0 . 2 % serum was much more inhibited by IGFBP 3 than by IgG anti IGF 2 ; 3 ) after separation of IGF 1 and IGF 2 from serum by chromatography of acidified serum proteins on BioGel P 150 , the remaining serum proteins ( with a molecular mass greater than 45 kDa ) which were depleted in IGF 1 and 2 ( verified by RIA determination ) still stimulated DNA synthesis , and this stimulation was 80 % inhibited by IGFBP 3 . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Cleavage of IGFBP 3 by PSA resulted in a marked reduction in the binding affinity of the fragments to IGF 1 , but not IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In animals given both bromocriptine and anti rGH , concurrent treatment with recombinant bovine GH maintained milk yield at 50 % of control values and normalized serum IGF 1 , IGF 2 and IGFBP 3 concentrations . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The insulin like growth factors , IGF 1 and IGF 2 , circulate in both humans and rats as part of a 125 150 kDa complex comprising IGFs , the IGF binding protein IGFBP 3 , and an acid labile subunit . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
When the IGFBPs from the high mol wt complex were cross linked to [ 125I ] IGF 1 or 2 , bands of 48 , 34 , 26 , and 21 kDa , which were more intense with [ 125I ] IGF 2 , were observed , and all were immunoprecipitable by anti IGFBP 3 antibody . ^^^ When purified alpha subunit or fractions from neutral Sephacryl S 300 chromatography were cross linked to covalent [ 125I ] IGF 2 : IGFBP 3 , a specific band at 150 kDa was observed with pure alpha subunit as well as with S 300 150 to 100 kDa serum fractions . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A procedure that combined ion exchange , gel permeation , and insulin like growth factor binding protein 3 ( IGF BP 3 ) affinity chromatography with chromatofocusing and reversed phase high pressure liquid chromatography was used to isolate high molecular weight precursors of human insulin like growth factor 2 ( IGF 2 ) from acetic acid extracts of Cohn fraction IV 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A RIA is described for the acid labile ( alpha ) subunit of the high mol wt insulin like growth factor ( IGF ) binding protein complex , a glycoprotein of approximately 85 , 000 daltons ( approximately 85K ) which combines with the GH dependent binding protein ( BP 53 or IGFBP 3 ) and IGF 1 or IGF 2 to form the complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Like GH , IGF 1 administration raises IGF 1 and two glycosylation variants of IGFBP 3 in the large complex , but unlike GH drastically reduces IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Hypophysectomy significantly decreased IGFBP 3 , IGF 1 , and IGF 2 in nonpregnant mouse serum , increased the amount of IGFBP 2 found in pregnant mouse serum , and had no effect on IGF 1 or 2 concentration in day 14 pregnant mouse serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Knowledge of the circadian patterns of serum IGF 2 and the large molecular weight IGF binding protein , IGFBP 3 might , apart from its physiological relevance , be of clinical interest , inasmuch as measurements of these parameters are being introduced into the evaluation of GH deficiency . ^^^ We therefore evaluated the 24 h ( 08 . 00 08 . 00 h ) patterns of serum IGF 2 and IGFBP 3 in 8 GH deficient patients who were studied during three periods when receiving 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The 140 kDa insulin like growth factor ( IGF ) binding protein complex in human serum consists of three subunits : an acid labile , non IGF binding glycoprotein ( alpha subunit ) , an IGF binding glycoprotein known as BP 53 or IGFBP 3 ( beta subunit ) , and IGF 1 or IGF 2 ( gamma subunit ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Virtually all circulating insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) are bound to specific binding proteins ( IGFBP ) , of which IGFBP 3 is the quantitatively most important . ^^^ We therefore evaluated the temporal association of serum IGF 1 , IGF 2 , and IGFBP 3 measured by RIAs after well defined short term GH exposure in GH deficient patients . ^^^ The molar ratio of serum IGF 1 plus IGF 2 : serum IGFBP 3 remained constant with values between 0 . 8 0 . 9 except in the constant infusion experiment , in which the ratio increased significantly with time reaching a mean peak value , which exceeded 1 . 0 , after 24 h . ^^^ The earlier increase in serum IGF 1 compared to IGF 2 and IGFBP 3 suggests that IGF 1 may be the main regulator of IGFBP 3 production . ^^^ Accordingly , the slow increase in serum IGF 2 , which paralleled that of IGFBP 3 , could indicate that serum IGF 2 levels mainly depend on the concentration or binding site availability of IGFBP 3 . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Coincubation of fibroblasts with TGF beta and either IGF 1 or IGF 2 at 50 ng / ml enhanced IGFBP 3 production 1 . 5 to 2 fold compared to TGF beta alone . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have examined the developmental pattern of IGF binding protein 1 ( IGFBP 1 ) , 2 , and 3 in serum by Western ligand blotting , the levels of IGFBP 3 , IGF 1 , and IGF 2 in serum by RIA , and the IGFBP mRNA levels of IGFBP 1 , 2 , and 3 in the livers of rhesus monkeys from fetal life through adulthood by Northern analysis . ^^^ The serum levels of IGF 1 and IGF 2 were strongly correlated with the level of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This combined analysis demonstrated an IGF 2 specific binding protein at approximately 250 kDa that was present in plasma and each lymph type and presumably represented the soluble type 2 IGF receptor ; a complex of 130 kDa containing 52 kDa and 46 kDa binding proteins that was labelled by all three IGF peptides was particularly evident in plasma and intestinal lymph and was probably a complex between IGFBP 3 and the acid labile subunit ; and a group of binding proteins that chromatographed as IGF complexes at approximately 50 kDa . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Tumor IGF 2 of 10 15 kDa circulates in a 60 kDa complex , in contrast to the ternary 150 kDa complex in which serum IGFs normally circulate together with the IGF binding subunit ( IGFBP 3 ) and the acid labile subunit ( alpha subunit ) . ^^^ Therefore , the cause of hypoglycemia in these patients may be the inability of complexes between the abnormal tumor IGF 2 and IGFBP 3 to be sequestered in the biologically inactive ternary complex . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The fetal expression pattern of high IGF 2 and IGFBP 2 is replaced by the adult pattern of low levels of IGF 2 and IGFBP 2 and high levels of IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
With the ligand blot analysis , [ 125I ] IGF 2 shows bands at 300 , 175 and 46 / 43 kilodaltons probably representing IGF 2 receptor and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Thus , molar ratios of IGFBP 3 / IGF 2 were consistently higher in old and WS cells and increased substantially as all three cell types became quiescent , due to either serum depletion or high cell density . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Since IGFBP 3 must bind IGF 1 or IGF 2 to form a complex with the acid labile subunit ( alpha subunit ) , we have used ternary complex formation from [ 125I ] alpha subunit as a measure of IGF binding to serum IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study was undertaken to investigate the presence of IGF 1 and IGF 2 , as well as that of their binding proteins ( BP ) , IGFBP 1 and IGFBP 3 in human serum and follicular fluid ( FF ) . ^^^ No clear conclusions can be drawn regarding the source of FF IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These mutants were tested for their binding affinities to type 1 and type 2 IGF receptors , to IGF binding protein 3 ( IGFBP 3 ) and for their stimulation of thymidine incorporation into DNA . [ Leu 27 ] IGF 2 exhibits an affinity to the type 2 IGF receptor close to that of wild type IGF 2 , but has lost completely the affinity to the type 1 IGF receptor . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A third minor species , of 22 kDa , with a preferential affinity for IGF 2 similar to that of the 34 kDa IGFBP , has an N terminal sequence , ( / ) ( / ) Asp Ser Phe Val Pro ( / ) Glu Pro Ser Asp Glu Lys Ala Leu Ser ( / ) ( / ) Pro , which bears some analogy with those of other known human IGFBPs , particularly IGFBP 3 ( 7 homologous residues ) , and appears to be related to , but distinct from , other IGFBP species . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that growth hormone ( GH ) insensitivity is responsible , amongst other mechanisms , for impaired growth in uraemic children , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 were measured by radioimmunoassay in normal control children , in patients with end stage renal failure ( n = 51 ) and in patients with preterminal chronic renal failure ( n = 11 ) and the production rate of IGF was calculated . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In contrast with binding proteins produced by normal human fibroblasts , TFBP does not cross react in radioimmunoassays for the plasma protein IGFBP 3 or the amniotic fluid protein IGFBP 1 ; however , after affinity labeling with IGF 2 , it is immunoprecipitable by an anti IGFBP 3 antiserum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have examined the IGFBPs secreted by monolayer cultures of neonatal fibroblasts by ligand blotting with [ 125I ] IGF 2 and immunoblotting with antisera raised against three well characterized IGFBPs : IGFBP 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We conclude that the 28K IGF binding protein from amniotic fluid , like the previously purified 53K binding protein , has high affinity for both IGF 1 and IGF 2 , that it coexists in amniotic fluid with BP 53 or a related protein , and that the levels of both proteins decline with increasing fetal maturity . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Pure BP 53 alone was inactive , but after preincubation with IGF 1 or IGF 2 , competed with a potency identical to that of BP 53 in acidified serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The proteins tested in reconstitution mixtures were an acid labile Mr 84 , 000 86 , 000 glycoprotein doublet ( alpha subunit ) , an acid stable Mr 47 , 000 53 , 000 glycoprotein doublet with IGF binding activity ( BP 53 or beta subunit ) , and IGF 1 or IGF 2 ( gamma subunit ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Comparison of IGF 1 and IGF 2 binding by fibroblast BP 53 revealed slightly higher IGF 2 than IGF 1 binding , and association constants of 3 4 10 10 ( 10 ) liter / mol for both IGFs , similar to BP 53 from human plasma . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Addition of rhIGFBP 3 to conditioned media inhibited 125I rhIGFBP 4 proteolysis by 90 % , while IGF 1 and IGF 2 reversed the inhibitory effects of rhIGFBP 3 in a dose dependent manner . 125I rhIGFBP 4 proteolysis was not inhibited by N terminal rhIGFBP 3 fragments that bind IGFs , but was inhibited by two synthetic peptides corresponding to sequences contained in the mid region or C terminal region of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study compared levels of the IGF 1 and IGF 2 , and of the IGF binding protein 2 ( IGFBP 2 ) and IGFBP 3 in vitreous from three groups with different degrees of retinal ischemia , as judged by the extent of neovascularization : a control group without new vessel formation , retinal neovascularization in patients with proliferative diabetic retinopathy , and massive ischemia of various causes resulting in rubeosis . ^^^ IGF 1 and IGFBP 3 were increased 10 and 13 fold in rubeosis ( P < < 0 . 01 ) compared with no ischemia ( n = 10 ) , while IGF 2 and IGFBP 2 were elevated 2 . 7 and 4 . 3 fold ( P < 0 . 01 ) . ^^^ Vitreous from patients with proliferative diabetic retinopathy but without rubeosis ( n = 16 ) contained 2 . 5 and 2 . 2 fold elevated levels of IGF 1 and of IGFBP 2 ( P < 0 . 05 ) , while IGF 2 and IGFBP 3 were increased 1 . 4 and 1 . 6 fold , which was not significant . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There was a positive correlation between height SDS and IGF 2 SDS ( r = 0 . 66 , P < 0 . 001 ) and IGFBP 3 ( r = 0 . 64 , P < 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report the relationship between serum IGF 1 , IGF 2 , their binding proteins IGFBP 1 and IGFBP 3 , and GH binding protein in 600 normal Spanish children who were divided into 5 groups according to Tanner stage : 1 , 150 males and 102 females ; 2 , 40 males and 42 females ; 3 , 45 males and 45 females ; 4 , 42 males and 55 females ; and 5 , 23 males and 56 females . ^^^ There is a close linear correlation between the sum of serum IGF 1 plus IGF 2 levels vs . serum IGFBP 3 ( r = 0 . 724 ; P < 0 . 0001 ) . ^^^ In contrast , there is a nonlinear correlation between serum IGF 1 vs . serum IGFBP 3 ( concave curve ) as well as between serum IGF 2 and serum IGFBP 3 ( convex curve ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have previously reported that adult GH receptor deficient ( GHRD ) patients treated subcutaneously with recombinant human insulin like growth factor ( IGF ) 1 have increased serum IGF 1 levels and decreased IGF 2 levels , whereas IGF binding protein 3 ( IGFBP 3 ) levels were unchanged . ^^^ To further investigate the effects of IGF 1 administration upon the IGF IGFBP axis in GHRD , we have examined : 1 ) the molecular distribution of IGF 1 and IGF 2 among the IGFBPs ; 2 ) the composition and distribution of the IGFBPs , in particular IGFBP 3 ; and 3 ) the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors 1 ( IGF 1 ) and 2 ( IGF 2 ) and their binding proteins ( IGFBP 1 , IGFBP 2 and IGFBP 3 ) were measured by radioimmunoassay in plasma and milk samples collected throughout the study . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Circulating insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) , when occupied by IGF 1 or IGF 2 , combines with an acid labile glycoprotein subunit ( ALS ) to form a high molecular weight complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This occurs without disrupting the 150 kilodalton ( kDa ) complexes in which IGFBP 3 carries of most of the IGF 1 and IGF 2 in serum . ^^^ Nevertheless , competitive binding experiments using [ 125I ] IGF 1 and 2 and IGFBP 3 preparations from each of the plasma pools showed that the competitive potencies of IGF 2 and especially IGF 1 were lower in pregnancy IGFBP 3 than in normal IGFBP 3 . ^^^ Scatchard analysis revealed a 2 fold loss of affinity for IGF 2 and a 10 fold loss for IGF 1 compared with that for normal plasma IGFBP 3 . ^^^ In studies at 37 C of the kinetics of dissociation of [ 125I ] IGF 1 and 2 bound to IGFBP 3 , IGF 2 was dissociated 6 times faster , and IGF 1 10 times faster from pregnancy plasma IGFBP 3 than from normal plasma IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
When milk production had ceased , as determined by litter weight loss and the absence of milk in the stomachs of pups , attempts were made to reinitiate lactation using prolactin , GH , insulin like growth factor 1 ( IGF 1 ) precomplexed to recombinant human IGF binding protein 3 ( hIGFBP 3 ) or IGF 1 plus IGF 2 precomplexed to hIGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report ( 1 ) an increase in serum IGF 2 concentrations ( about 2 fold ) , whereas IGF 1 concentrations are regarded as normal when aging is taken into account ; ( 2 ) an apparent increase in serum IGFBP 3 levels when compared to those of healthy elderly subjects , IGFBP 3 only being detected in the 150 kDa IGFBP ternary complex as in normal serum ; ( 3 ) abnormally elevated serum IGFBP 2 levels taking into account the apparent concentrations of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the relation between normal human fetal growth and the levels of insulin like growth factors ( IGF 1 , IGF 2 ) , their receptors , and IGF binding protein 3 ( IGFBP 3 ) in both the maternal and fetal compartments . ^^^ Aliquots of amniotic fluid ( AF ) and serum samples were analyzed for levels of IGF 1 , IGF 2 , and IGFBP 3 . ^^^ There were no significant correlations between newborn weights and IGFBP 3 , or maternal serum levels of IGF 1 and IGF 2 . ^^^ CONCLUSION : These data demonstrate the presence of type 1 receptors and the bioavailability of IGF 1 , IGF 2 , and IGFBP 3 throughout pregnancy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In all subjects a single blood sample was drawn for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 and GH measurements by specific RIAs . ^^^ As IGF 2 is normal and IGFBP 1 and IGFBP 2 are inversely correlated to the serum GH levels in the acromegalic patients , we speculate that the molar ratio between IGF 1 and IGFBP 3 reflects free ( biologically active ) IGF 1 and is dependent on GH levels . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Treatment with IGF 2 increased the level of IGFBP 3 , as did IGF 1 or GH treatment , as shown by Western ligand blotting . ^^^ In conclusion , IGF 1 and IGF 2 as well as GH have a stimulatory effect on general body growth and are effective in the stimulation of serum IGFBP 3 , sulfate incorporation into cartilage , as well as the growth of specific organs in Snell dwarf mice . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Intact IGFBP 3 , as assessed by 125I labeled IGF 2 ligand blot analysis , is more abundant in culture media of cells exposed to growth hormone ( GH ) or to parathyroid hormone ( PTH ) , both at 5 10 10 ( 9 ) mol / l , for 24 h . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No evidence for reduced spontaneous or growth hormone stimulated serum levels of insulin like growth factor ( IGF ) 1 , IGF 2 or IGF binding protein 3 in women with spinal osteoporosis . ^^^ To test the hypothesis that a dysfunctional growth hormone ( GH ) insulin like growth factor ( IGF ) axis may play a role in the pathogenesis of osteoporosis , we compared the levels of IGF 1 , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) in 15 women with spinal osteoporosis ( i . e . at least one non traumatic vertebral fracture ) and 15 normal age matched women . ^^^ The basal levels of IGF 1 , IGF 2 and IGFBP 3 were similar in patients and controls ( mean + / SEM ) : IGF 1 , 16 . 5 + / 1 . 3 versus 16 . 0 + / 1 . 3 nmol / l ( NS ) ; IGF 2 , 79 . 9 + / 3 . 6 versus 72 . 5 + / 4 . 1 nmol / l ( NS ) ; and IGFBP 3 , 125 . 7 + / 6 . 5 versus 130 . 3 + / 7 . 8 nmol / l ( NS ) . ^^^ Stimulation with r hGH elicited increased levels of IGF 1 , IGF 2 and IGFBP 3 within both groups ( p < 0 . 001 ) . ^^^ The maximal values expressed as a percentage of baseline were : IGF 1 , 341 + / 26 % versus 369 + / 22 % , IGF 2 , 125 + / 4 % versus 119 + / 5 % , IGFBP 3 , 141 + / 5 % versus 147 + / 7 % in osteoporotic patients and controls , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We investigated IGF 1 , IGF 2 , and IGFBP 3 levels by specific RIAs in CSF from patients with central nervous system ( CNS ) tumor or leukemia and compared them with values in patients with meningitis . ^^^ The CSF from 38 children who received lumbar punctures to exclude meningitis was used to define the normal range for IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 protease activity in CSF . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have therefore examined the effects of recombinant human IGF 1 ( rhIGF 1 ) administration on the levels of IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 and IGF bioactivity in a group of 9 late pubertal adolescents with IDDM . ^^^ There was a small increase in IGFBP 3 concentrations overnight following rhIGF 1 administration when compared to placebo , whereas the levels of IGF 2 decreased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Of the total circulating IGF 1 and IGF 2 , 70 % was carried by the 150 kDa complex in normal subjects , while in patients with GHRD , 50 % of serum IGF 1 , but only 30 35 % of serum IGF 2 , was measured within the 150 kDa IGFBP 3 region . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have recently suggested that the bioavailability of circulating IGF 1 is increased in the human fetus due to the molar excess of IGF 1 plus IGF 2 relative to IGFBP 3 as well as the increased concentrations of IGFBP 2 , which does not form a long lived ternary complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of IGFBP 3 and IGFBP 4 tended to be higher in phosphoenolpyruvate carboxykinase IGF 2 transgenic mice than in controls , as evaluated by ligand blot analysis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Spontaneous overnight and arginine stimulated GH secretion , insulin , IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 levels were measured before , during , and after daily 10 h sc infusions of saline or IGF 1 ( 20 micrograms / kg . h ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Low mol wt fragments of IGFBP 3 , which did not bind to IGF 1 or IGF 2 on Western ligand blot and did not cross link to IGF 2 , were demonstrated on Western immunoblot and were measurable by two different RIAs . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
While the affinity of IGF 2 for the PSA generated IGFBP 3 fragments fell slightly compared to intact IGFBP 3 , the affinity of the PSA generated IGFBP 3 fragments for IGF 1 fell by ten fold . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Acidification of both whole serum and purified ALS leads to a complete loss of ALS ability to bind to cross linked IGFBP 3 : [ 125I ] IGF 2 tracer ; however , immunoblot studies show no change in the apparent M ( r ) of the major ALS band . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Fetal serum concentrations of IGFBP 3 , IGF 1 , and IGF 2 were low with marked or only modest increases with gestational age ( p < 0 . 001 , p < 0 . 005 , and p < 0 . 05 , respectively ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A major portion of the 150 kilodalton insulin like growth factor binding protein ( IGFBP ) complex in adult rat serum contains unoccupied , proteolytically nicked IGFBP 3 that binds IGF 2 preferentially . ^^^ The acid eluate and the acid stripped flow through predominantly formed 50 kDa complexes with [ 125I ] IGF 2 when affinity cross linked and examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis ; these complexes were precipitated by antibodies to rat IGFBP 3 . ^^^ We conclude that the 150 kDa fraction of adult rat serum includes 1 ) ternary complexes of intact IGFBP 3 ( which binds IGF 1 and IGF 2 with similar affinity ) , endogenous IGF 1 , and the ALS ; and 2 ) binary complexes of proteolytically nicked IGFBP 3 and ALS that bind IGF 2 preferentially . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We investigated the responses of these cells to exogenously added IGF 1 , IGF 2 , or IGFBP 3 . ^^^ When IGFBP 3 was administered together with IGF 1 or IGF 2 , it further increased the mitogenic response observed in PC 3 ARCA cells , but no effect on PC 3 Neo cells was observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 , the major circulating IGFBP , is unique in combining with a glycoprotein , the acid labile subunit ( ALS ) , to form a ternary complex with IGF 1 or IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN : The circulating concentrations of GH , IGF 1 , IGF 2 and IGFBP 3 were measured in acromegalic patients before and after 3 , 6 , 9 , and 12 months of treatment with octreotide ( group 1 : n = 5 ) , and compared with those found in a group of patients ( group 2 ) treated with bromocriptine ( n = 3 ) , cabergoline ( n = 7 ) radiotherapy ( n = 3 ) or surgical therapy ( n = 2 ) . ^^^ RESULTS : Before treatment , the concentration of IGF 1 and IGFBP 3 were above the normal range in all patients , while IGF 2 levels were slightly reduced . ^^^ After treatment with octreotide , IGF 1 ( P = 0 . 004 ) , IGF 2 ( P = 0 . 02 ) and IGFBP 3 ( P < 0 . 001 ) were significantly reduced as compared to basal levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We examined excretion of urinary insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) and their major binding protein IGFBP 3 in comparison to their respective serum concentration in nine healthy female volunteers ( median age 25 years , range 22 27 ) under baseline conditions and after stimulation with recombinant human growth hormone ( rhGH ) , 4 . 5 IU twice daily subcutaneously for a period of 3 days . ^^^ The mean ( + / SD ) urinary concentrations of IGF 1 ( 0 . 08 + / 0 . 07 micrograms / l ) , IGF 2 ( 1 . 02 + / 0 . 47 micrograms / l ) and IGFBP 3 ( 19 . 1 + / 6 . 9 micrograms / l ) were two to three orders of magnitude lower than in serum . ^^^ The ratio of IGF 2 over IGF 1 concentration in urine ( 13 : 1 ) was five times higher than in serum ( 2 . 5 : 1 ) , and the ratio of IGFBP 3 over the sum of IGF 1 and IGF 2 in urine ( 17 : 1 ) was four times higher than in serum ( 4 : 1 ) . ^^^ Urinary excretion was 63 . 3 + / 46 . 6 ng . m 2 . 24h 1 for IGF 1 , 1002 + / 598 ng . m 2 . 24h 1 for IGF 2 and 18039 + / 4983 ng . m 2 . 24h 1 for IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the treated diabetics IGF 1 and IGF 2 were reduced ; IGFBP 2 ( only in prepubertal subjects ) and IGFBP 3 were increased . ^^^ Furthermore , age adjusted values of IGF 1 , IGF 2 , and IGFBP 3 were lower in prepubertal than in pubertal patients . ^^^ Strong correlations were observed between height SDS and IGF 1 SDS , IGF 2 SDS , and IGFBP 3 SDS in prepubertal subjects who had had IDDM for at least 2 yr , but not in adolescents . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Before insulin therapy , serum IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , and GH binding protein ( GHBP ) levels were significantly decreased , whereas IGFBP 1 and cortisol were significantly increased in diabetic children compared to those in an age , sex , and stage of puberty matched control group . ^^^ IGF 2 , IGFBP 3 , and GHBP showed a slower pattern of change , with their levels reaching control values only 1 month after the start of insulin treatment . ^^^ Improvement in glycemic control , as determined by a change in hemoglobin A1c , correlated positively with improvement in IGF 1 , IGF 2 , IGFBP 3 , GHBP , and weight gain after 1 month of insulin therapy . ^^^ The decreases in IGF 1 , IGF 2 , and IGFBP 3 may in part be due to a decrease in the GHBP / receptor . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Blood , collected from a cohort ( n = 3 ) of these patients within 24 h of their discharge from the hospital , indicated that IGF 1 and IGF 2 were still reduced ( 30 % ) and that the decrease in IGFBP 3 and the elevation in IGFBP 1 were still evident at this time . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The biological activity of the IGF 2 peptide was further demonstrated by increased serum IGF binding protein 3 in the transgenic dwarf mice , as shown by Western ligand blotting . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In normal term FCS , the following levels ( + / SE ) were present : IGF 1 , 76 + / 16 ; IGF 2 , 401 + / 38 ; IGFBP 3 , 700 + / 112 ; IGFBP 1 , 77 + / 10 ng / mL ; and insulin , 3 . 8 + / 1 . 6 microU / mL . ^^^ In IUGR FCS , IGF 1 , IGF 2 , and IGFBP 3 were significantly reduced , and IGFBP 1 was 7 fold higher than in FCS from normal weight fetuses . ^^^ In contrast , IGF 2 and IGFBP 3 levels did not significantly change during the first week of life in preterm or term newborns . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : The aim of this investigation was to study the effect of relatively high dose IGF 1 therapy given for several months , on serum levels of IGF 1 , IGF 2 and IGFBP 3 , and on IGF 1 pharmacokinetics in patients with growth hormone insensitivity due to GH receptor dysfunction . ^^^ MEASUREMENTS : Serum was sampled at baseline and at 3 8 months , for determination of IGF 1 , IGF 2 and IGFBP 3 by radioimmunoassay , and for evaluation of IGFBPs and IGFBP 3 protease activity by Western ligand blot and protease assay , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In support of this hypothesis , we have demonstrated using Hs578T cells that : 1 ) TGF beta stimulates IGFBP 3 gene expression and production prior to its inhibition of cell growth , 2 ) treatment with an IGFBP 3 antisense oligodeoxynucleotide selectively inhibits TGF beta induced IGFBP 3 synthesis and cell growth inhibition , and 3 ) treatment with IGF 2 and IGF 2 analogs diminish TGF beta effects by blocking TGF beta induced binding of IGFBP 3 to the cell surface . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
All groups experienced a significant increase in serum IGF 1 and IGFBP 3 levels over the treatment period , accompanied by significant decreases in IGF 2 ( P < 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 and IGFBP 6 were used to study whether both IGF 1 and IGF 2 play a role in auto / paracrine stimulation of rat osteoblast growth . ^^^ Consistent with their IGF binding affinities , IGFBP 3 blocked both IGF 1 and IGF 2 stimulated DNA synthesis , whereas IGFBP 6 preferentially blocked IGF 2 stimulated DNA synthesis . ^^^ Because IGFBP 6 preferentially binds IGF 2 and is much less potent than IGFBP 3 in decreasing basal DNA synthesis in calvaria cells , IGF 1 but not IGF 2 appears to be an important auto / paracrine stimulator of DNA synthesis . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 was in the normal range and increased significantly during GH treatment , although relatively less than IGF 1 , such that the molar ratio between IGF 1 and IGFBP 3 increased with GH treatment , whereas the ratio between IGF 1 + IGF 2 and IGFBP 3 remained unchanged . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) in healthy infants , children , and adolescents : the relation to IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , age , sex , body mass index , and pubertal maturation . ^^^ Therefore , we measured IGFBP 3 , IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 2 levels by RIA in 907 healthy children to establish well characterized normative data on IGFBP 3 according to age , sex , and pubertal stage and to study the complex relationship between IGFs and their BPs in puberty . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Lastly , IGFBP 2 and IGFBP 3 are mitogenic for UMR 106 . 01 rat osteosarcoma cells , inducing an increase in cell number to 125 % and 142 % of control cell counts after 48 h of incubation with 1000 ng / ml IGFBP 2 and 3 , whereas IGF 1 , IGF 2 and Long R 3 IGF 1 did not stimulate proliferation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 94 children with chronic renal failure ( CRF ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Compared with normal IGF 2 , pro IGF 2 inhibited ALS binding to IGFBP 3 in vitro . ^^^ Although prednisolone and GH reverse hypoglycemia by different mechanisms , with only prednisolone suppressing tumor IGF 2 secretion , both increase the formation of ternary IGF IGFBP 3 complexes . ^^^ We conclude that the failure of serum IGFBP 3 and tumor IGF 2 to complex with ALS is a primary cause of hypoglycemia in nonislet cell tumor hypoglycemia . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To test the hypothesis that human bone cells ( HBCs ) , which produce abundant amounts of IGF 2 and IGFBP 3 , also produce ALS , we measured the IGF 1 , IGF 2 , IGFBP 3 , and ALS levels using specific radioimmunoassays ( RIAs ) in the conditioned medium ( CM ) of untransformed normal HBCs and SaOS 2 osteosarcoma cells treated with various effectors ( IGF 2 , osteogenic protein 1 [ OP 1 , bone morphogenetic protein 7 ] and human GH ) for 48 h . ^^^ In the IGF 2 ( 3 , 30 ng / ml ) treated HBC CM , the IGFBP 3 level was increased in a dose dependent manner but neither IGF 1 nor ALS could be detected . ^^^ In the SaOS 2 cell culture , OP 1 ( 1 , 100 ng / ml ) increased both IGF 2 and IGFBP 3 secretion but neither ALS nor IGF 1 secretion . ^^^ Treatment of HBCs with GH ( 1 , 10 , 100 ng / ml ) had no significant effect on the secretion of either IGF 1 , IGF 2 , IGFBP 3 , or ALS . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Preincubation of fresh medium with recombinant IGFBP 1 or IGFBP 3 in the presence of IGF 2 suppressed IGF 2 stimulation of glycogen synthesis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We conclude from these results 1 ) that IGF 2 is involved in the autocrine control of PC 3 cell proliferation via the type 1 IGF receptor ; and 2 ) that this proliferation is directly dependent on IGF 2 bioavailability that itself is modulated by the limited IGFBP 3 proteolysis induced , at least in part , by urokinase type plasminogen activator and plasmin . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Initially , growth hormone secretion was suppressed , and she had low levels of insulin like growth factor ( IGF ) 1 and IGF binding protein 3 , but elevated levels of large molecular weight IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In addition to IGFBP 2 , a 35 45 kDa IGFBP identified by immunoprecipitation as IGFBP 3 was variably present on ligand blots of control conditioned medium and was consistently found in medium from cells treated with IGF 1 or IGF 2 ( ED 50 3 ng / mL ) but not with insulin at 2 mg / mL . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGFBP 3 levels determined by WLB and serum IGF 1 and IGF 2 levels measured by RIA after acid chromatography were not different among the subjects with cancer and the normal controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have confirmed that IR IGF 1 levels are low in hypothyroidism and have demonstrated a reduction in IGF bioactivity and IGF 2 and IGFBP 3 levels , and low GH binding activity , which may reflect a reduction in the processing of GH receptors . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The inhibitory effects of IGFBP 3 were greater for IGF 2 than for IGF 1 when IGFBP 3 was coincubated with either of the IGFs , at both IGF concentrations of comparable activity and equivalent molar concentrations . ^^^ Interestingly , when cells were pretreated with IGFBP 3 , prior to the simultaneous addition of IGFs and IGFBP 3 , the inhibitory effect was higher for IGF 1 than for IGF 2 . ^^^ IGFBP 3 exhibited an ED 50 low enough to suppress IGF 2 and possibly IGF 1 action when cells were pretreated with IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We also studied the effects of hGH on osteoblastic production of insulin like growth factor 1 ( IGF 1 ) and IGF 2 as well as the production of GH dependent , insulin like growth factor binding protein 3 ( IGFBP 3 ) . ^^^ Under basal conditions , human osteoblasts produced IGF 2 and IGFBP 3 in the conditioned medium . ^^^ When stimulated with hGH , minor insignificant increase in both IGF 2 and IGFBP 3 ( 125 % and 126 % of control , respectively ) were detectable . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In general , there was an inverse relationship with serum IGFBP 3 ( except IDDM ) and IGFBP 2 levels were high in situations where free IGF 2 should be expected to be high . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Northern blots of total cellular RNA were probed with oligonucleotides for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and a human IGFBP 1 cDNA . ^^^ The data showed that in fibroids compared to myometrium , 1 ) the relative abundance of IGF 1 mRNA was not different , but there was an increase in the relative abundance of IGF 2 mRNA ( P < 0 . 001 ) ; 2 ) IGFBP 1 mRNA was undetectable in fibroids and detectable in only 1 specimen of myometrium ; 3 ) there was no difference in the relative abundance of IGFBP 2 mRNA , but there was an increase in the relative abundance of IGFBP 3 mRNA in myometrium ( P < 0 . 05 ) . ^^^ Our data show that the mRNAs encoding IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 are expressed in both fibroids and myometrium and that fibroids express more IGF 2 and less IGFBP 3 mRNA than myometrium . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The aim of this study was to assess urinary IGF 1 , IGF 2 , and IGFBP 3 in a cross sectional study of healthy subjects , as well as characterize urinary IGFBPs ( uIGFBps ) in patients with GH deficiency ( GHD ) and renal disease , such as , Alport syndrome , immunoglobulin A nephropathy , focal segmental glomerulosclerosis , and systemic lupus erythematosus . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Tumor big IGF 2 and IGF 2 were equally effective in forming the 150 kDa complex with purified IGFBP 3 and 125I labeled alpha subunit . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Immunoreactive IGFBP 3 was increased in proportion to IGF 1 and the molar ratio [ IGFBP 3 ] : [ IGF 1 ] + [ IGF 2 ] was close to unity ( 1 . 04 + / 0 . 14 ) , as that of healthy subjects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The mean fasting levels of glucose and insulin were higher , whereas the mean basal IGF 1 , IGF 2 , and IGFBP 3 levels were lower in the endometrial cancer patients than in the healthy control subjects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DBcAMP treatment of LSaOS cells decreased IGF 2 and IGFBP 3 but increased IGFBP 4 and type 1 IGF receptor ; no effect was observed for the type 2 IGF receptors . ^^^ DBcAMP treatment of HSaOS cells had no detectable effect on IGF 2 ; IGFBP 3 , or type 1 and type 2 IGF receptor expression ; only IGFBP 4 expression increased with DBcAMP . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Ternary complex formation has been measured by the binding of the acid labile subunit of the circulating IGFBP 3 complex , which also requires IGF 1 or IGF 2 binding . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Cleavage of IGFBP 3 results in the appearance of a 31 kDa fragment that is 50 fold reduced in its affinity for the IGF 1 or IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
After 24 h of treatment , IGF 1 , IGF 2 , and high concentrations of insulin increased IGFBP 2 and IGFBP 3 levels . ^^^ In situ hybridization studies demonstrated an increase in the abundance per cell of IGFBP 2 and IGFBP 3 mRNAs in either one or both types of astroglial cells after IGF 1 or IGF 2 treatment . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) forms a 140 kilodalton ( kDa ) complex with an acid labile glycoprotein after complexing with IGF 1 or IGF 2 . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) forms a 140 kilodalton ( kDa ) complex with an acid labile glycoprotein after complexing with IGF 1 or IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The somatotropin somatomedin axis in adult patients with Turner syndrome : measurement of stimulated GH , GH BP , IGF 1 , IGF 2 and IGFBP 3 in 25 patients . ^^^ No significant differences were present when the integrated GH response to stimulation with arginine and the serum levels of GH binding protein ( GH BP ) , IGF 1 , IGF 2 and binding protein 3 for IGFs ( IGFBP 3 ) were compared between the two groups . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the patients with GHIS , chronic IGF 1 treatment , with twice daily injections for 6 months , caused a significant steady decline of IGF 2 and an increase in IGFBP 2 , but had no effect on IGFBP 1 and IGFBP 3 levels . ^^^ The data suggest that total IGF 1 and IGF 2 serum levels are determined mainly by IGFBP 3 , even in extreme situations such as GHIS , while other IGFBPs are less important . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
It has previously been demonstrated that 17 parents heterozygous for the Ecuadorean mutation of the GH receptor differed little in biochemical measures ( GHBP , IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 ) from Ecuadorean controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied plasma gonadotropins , sex steroids , adrenal androgens , and insulin like growth factors ( IGF 1 and IGF 2 ) and their binding protein 3 ( IGFBP 3 ) in a large number of WBS patients from infancy to adulthood ( n = 23 females , n = 33 males ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To further our understanding of the growth failure in metabolic acidosis , we examined the insulin like growth factor ( IGF 1 and IGF 2 ) , the IGF binding protein 3 ( IGFBP 3 ) , and the hepatic IGF mRNA and growth hormone receptor mRNA in control , pair fed and acidotic rats . ^^^ The serum IGF 2 and IGF binding protein 3 concentrations were unchanged by acidosis or food restriction . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As expected , IGF 1 and IGF 2 concentrations in serum correlated positively with the serum concentration of IGFBP 3 , the major IGF binding protein in serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report that while TNF alpha had no consistent effect on the levels of IGF 1 , IGF 2 , or on IGF 1 receptor ( protein and messenger RNA ( mRNA ) ] , it stimulated IGFBP activity and particularly IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
During pregnancy in the baboon , this ratio is perturbed ; serum IGFBP 3 concentrations increase 10 fold , yet IGF 1 levels are unaltered and IGF 2 is increased only 2 fold ( Giudice et al . 1993 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In this study we measured the concentrations of IGF 1 , IGF 2 , and IGFBP 3 in normal human synovial fluid by RIA and characterized the IGFBPs by Western ligand blot ( WLB ) , Western immunoblot , and immunoprecipitation . ^^^ The concentrations of IGF 1 , IGF 2 , and IGFBP 3 in normal synovial fluid were 19 + / 3 ( mean + / SE ) , 194 + / 14 , and 349 + / 65 ng / mL , respectively . ^^^ In synovial fluid of patients with OA , IGF 1 levels were elevated , whereas IGF 2 was decreased , and the IGFBP 3 level was similar to the control value . ^^^ In patients with RA , both IGF 1 and IGFBP 3 were elevated , whereas IGF 2 remained unchanged . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Fetal blood samples ( FBS ) were collected for complete blood counts ( CBCs ) , hematopoietic progenitor assay , circulating insulin like growth factors ( IGF 1 , IGF 2 ) and binding proteins ( IGFBP 3 ) ( GD 120 , 140 , 153 + / 1 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Altered insulin like growth factor 2 ( IGF 2 ) level and IGF binding protein 3 ( IGFBP 3 ) protease activity in interstitial fluid taken from the skin lesion of psoriasis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 and IGF 2 in Hyp mice were not significantly different from those in normal mice , but IGFBP 3 levels were significantly lower ( 70 % of normal , p < 0 . 05 ) in the mutant strain . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) 1 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 are expressed in the rat brain in regions of neuronal loss by 3 days after hypoxic ischemic ( HI ) brain injury and IGF 2 somewhat later . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In PRL and GH deficient rats , PRL treatment could prevent all of these effects , GH was partially effective , whereas putative mediators of GH action , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 3 , were ineffective . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No detectable IGFBP 3 was observed by WLB with [ 125I ] IGF 1 as the ligand , but with IGF 2 as the ligand , IGFBP 3 fragments at 16 17 kDa were noted . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
They had characteristic features and biochemical findings including prominent forehead ; depressed nasal bridge ; central adiposity ; high pitched voices ; micropenis ; high GH levels and low levels of insulin like growth factor ( IGF ) 1 , IGF 2 , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and GH binding protein ( the solubilised extracellular domain of the GH cell surface receptor ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We examined here the associations between the plasma levels of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , and sex hormones in a population based sample of 486 men and women , aged 50 80 yr . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 , but not insulin , led to a much higher concentration of IGFBP 3 in the CM of all tested NSCLC cell lines , whereas the level of immunologically detected membrane associated IGFBP 3 was decreased . ^^^ Furthermore , Northern analysis of mRNA isolated from A 549 revealed that IGFBP 3 specific mRNA was not changed by IGF 1 or IGF 2 , suggesting that the IGF induced effects on IGFBP 3 depend on the release of cell associated IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , insulin and C peptide were measured by radioimmunoassay . ^^^ There was a close correlation between IGFBP 3 levels and the sum of IGF 1 and IGF 2 levels before surgery ( r = 0 . 9 , P < 0 . 01 ) and this was maintained throughout the post operative period ( mean correlation coefficient of 0 . 86 + / 0 . 02 , P < 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ The addition of 0 . 1 mol / l NaCl to 50 mmol / l phosphate buffer increased the affinity of IGF 2 for IGFBP 3 by 2 fold , and of IGF 1 by 4 fold . ^^^ Under ' physiological ' conditions , the affinities of ALS binding to IGF 1 IGFBP 3 and IGF 2 IGFBP 3 complexes ( 2 . 5 10 10 ( 8 ) l / mol and 5 . 8 10 10 ( 7 ) l / mol , respectively ) are 300 fold and 2000 fold lower than the constants for the formation of the corresponding binary complexes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , and IGFBP 3 were studied in the rhesus ( Macaca mulatta ) and long tailed ( Macaca fascicularis ) monkey fetus and dam during the second and third trimesters of pregnancy . ^^^ Thus , although IGF 1 , IGF 2 , and IGFBP 3 remained relatively unaffected in maternal sera during this period of gestation , fetal concentrations of IGF 1 , IGF 2 , and IGFBP 3 increased in a developmental profile similar to the human fetus . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Scatchard analysis of the data indicated a 20 fold loss of affinity for IGF 2 and an 50 fold loss for IGF 1 compared with that of recombinant human IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : AIDS wasting is associated with a GH resistant state that results in low levels of serum IGF 1 , IGF 2 and IGFBP 3 , elevated levels of phosphorylated IGFBP 1 , and a reduced ability to form the IGFBP 3 ternary complex . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Chondrocytes from osteoarthritic cartilage have increased expression of insulin like growth factor 1 ( IGF 1 ) and IGF binding protein 3 ( IGFBP 3 ) and 5 , but not IGF 2 or IGFBP 4 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the current study we performed daily IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 measurements in 9 healthy regularly cycling volunteers throughout the menstrual cycle . ^^^ In addition , we investigated IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 levels in 13 samples of androgen dominant follicular fluid [ FFa androstenedione to estradiol ( AD : E 2 ) ratio , > 4 ] and 19 samples of estrogen dominant follicular fluid ( FFe ; AD : E 2 ratio , 4 ) obtained from 21 regularly cycling subjects and in 18 samples of fluid from luteinizing follicles obtained from patients undergoing in vitro fertilization ( IVF ) treatment ( FFivf ) . ^^^ IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 were measured using two site immunoradiometric assays . ^^^ No significant day to day differences were observed in IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 levels across the menstrual cycle . ^^^ In contrast , significantly lower IGFBP 1 levels were found in FFe compared to FFivf ( 79 ng / mL ; range , 57 234 ; P = 0 . 002 ) , whereas there was no significant difference between FFe and FFivfe IGF 1 , IGF 2 , or IGFBP 3 levels , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In order to investigate the IGF regulatory pathway in leukaemia serum levels of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in 28 leukaemic children . ^^^ Whereas serum levels of IGF 1 ( mean / range : 2 . 7 / 0 . 1 to 6 . 7 SDS ) , IGF 2 ( 3 . 6 SDS / 1 . 3 to 8 . 7 ) and IGFBP 3 ( 2 . 0 / +2 . 2 to 7 . 1 SDS ) were significantly decreased comparable to levels in growth hormone deficiency , IGFBP 2 levels ( +4 . 0 / 0 . 45 to +7 . 4 SDS ) were found to be markedly elevated and inversely correlated to IGF 1 ( r = 0 . 51 , P = 0 . 013 ) . ^^^ CONCLUSION : This study demonstrates that the proliferation of malignant lymphoblasts ( at diagnosis vs treatment ) occurs in the presence of decreased serum levels of IGF 1 , IGF 2 and IGFBP 3 and that diminished production of these peptides may contribute to impaired growth . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The effects of sex and age on patterns of circulating somatotropin ( ST ) concentration and plasma IGF 1 , IGF 2 , insulin , and IGF binding protein 3 ( IGFBP 3 ) were studied in ram , wether , and ewe lambs ( n = 7 or 8 ) sampled at mean ages of 81 ( I 1 ) and 158 d ( I 2 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The normal range for serum IGF 2 in women was 490 1056 micrograms / L , and IGF 2 levels were positively correlated with serum concentrations of insulin like growth factor binding protein 3 ( IGFBP 3 ) but not with IGF 1 levels . ^^^ Mean serum concentrations of IGF 2 were reduced below normal in a number of hypopituitary patients and children with short stature and IGF 2 concentrations in these subjects correlated positively with IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured in 54 children with end stage renal disease ( ESRD ) . ^^^ Affinity cross linking of 125iodine IGF 2 to sera from patients with ESRD and immunoprecipitation with a specific antiserum showed that low molecular weight IGFBP 3 fragments in ESRD serum are capable of binding IGF . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have therefore measured IGF 2 , as well as IGF 1 , and IGFBP 3 , levels in a large cohort of subjects with IDDM and in age / sex matched controls . ^^^ MEASUREMENTS : Random blood samples were obtained from each subject for the measurement of IGF 2 , IGF 1 and IGFBP 3 levels . ^^^ Multiple regression analysis revealed significant correlations between IGF 2 and IGFBP 3 ( F = 20 . 1 , P = < 0 . 001 ) and reaffirmed previously observed associations for IGF 1 and IGFBP 3 . ^^^ The sum of IGF 1 and IGF 2 ( expressed as nmol / l ) correlated with IGFBP 3 ; r = 0 . 47 in controls and 0 . 60 in diabetics . ^^^ The binding of IGFBP 3 for both IGF 1 and IGF 2 is unaltered by insulin dependent diabetes mellitus . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum growth hormone , insulin like growth factor ( IGF ) 1 , IGF 2 , and IGF binding protein 3 levels in normally grown and growth retarded human fetuses during the second half of gestation . ^^^ The normally grown group showed a progressive decline in serum GH levels toward term ( r = 0 . 42 , p = 0 . 0001 ) , whereas serum IGF 1 was increased ( r = 0 . 55 , p = 0 . 0001 ) , as were serum IGF 2 ( r = 0 . 21 , p = 0 . 008 ) and IGFBP 3 levels ( r = 0 . 19 , p = 0 . 02 ) , although less markedly . ^^^ No differences were found between the groups in serum GH , IGF 2 , and IGFBP 3 levels , although if data for the third trimester were taken alone , serum IGF 2 levels were found to be lower in the growth retarded group ( p = 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There were significant correlations between the CRP level and levels of IGF 1 , IGF 2 , and IGFBP 3 in the RA patients ( r = 0 . 62 0 . 898 , P = 0 . 04 0 . 0007 ) . ^^^ Levels of IGF 1 IGF 2 , and IGFBP 3 all correlated with the CRP level in patients with RA , which indicates the possibility that the IGF system is involved in the disease process . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the circulation , most of the IGFs are bound to a high molecular weight binding protein complex of 150 kDa that consists of IGF 1 ( or IGF 2 ) , IGFBP 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Chronic subcutaneous administration of Long R3IGF 1 , however , reduced growth rates , led to a depression in food intake and lowered concentrations of IGF 1 , IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Recent studies demonstrate that IGF 2 in FFe is higher than in FFa ' whereas IGF 1 , IGFBP 3 and IGFBP 1 levels do not differ , underscoring the importance of local IGF 2 production by the granulosa and the importance of IGFBP 4 and IGFBP 2 in regulation of IGF 2 action within the follicle during its developmental pathway as an E 2 or A dominant follicle . ^^^ In the androgen treated female to male transsexual ( TSX ) model for PCOS , IGF 1 , IGF 2 , IGFBP 3 and IGFBP 1 levels do not differ . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The effects of GH are believed to be mediated via IGF 1 , however , we were unable to mimic the effects of GH with IGF 1 , IGF 2 or a combination of IGF 1 , IGF 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Additionally , the role of IGFBP 3 in the antiproliferative effects of transforming growth factor ( TGF ) beta and retinoic acid ( RA ) is supported by our observations that : ( 1 ) inhibition of IGFBP 3 gene expression using an IGFNBP 3 antisense oligodeoxynucleotide not only blocks TGF beta and RA simulation of IGFBP 3 production by up to 90 % m but also inhibits their antiproliferative effects by 40 60 % ; and ( 2 ) treatment with IGF 2 and IGF 2 analogs diminish TGF beta effects by blocking TGF beta induced binding of IGFBP 3 to the cell surface . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Wilms tumor cell growth is also arrested by IGF binding protein 3 , capturing the continuously produced IGF 2 , and by alpha IR 3 , a type 1 IGF receptor blocking antibody . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 , an abundant IGFBP that binds IGF 1 and IGF 2 with high affinity , can form soluble inhibitory complexes with the IGFs that prevent them from binding to IGF 1 receptors . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These data show that : 1 ) the IGF peptide is less active when bound to IGFBP 3 ; 2 ) as IGFBP 3 does not affect the potency of des ( 1 3 ) IGF 1 , its inhibitory action is exerted upstream of the membrane receptor binding ; 3 ) as the action of IGFBP 3 is exerted by binding the IGF peptide , its inhibitory effect on FSH points out the role of the locally produced IGF 2 in potentiating the FSH action on human GC . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
After 10 min of high intensity exercise , all measured components of the IGF system were increased : IGF 1 by 13 . 3 + / 3 . 2 % ( P < 0 . 002 ) , IGF 2 by 15 . 7 + / 3 . 1 ( P < 0 . 01 ) , and IGFBP 3 by 23 + / 6 % ( P < 0 . 001 ) . ^^^ In summary : 1 ) brief exercise leads to small but significant increases in circulating IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 proteolysis ; and 2 ) these responses may be influenced by exercise intensity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGF 2 , and IGFBPs 1 4 , were readily identified by specific immunoassays and / or 125iodine IGF ligand blotting of simultaneously obtained PD and serum samples from seven CRF children ; IGFBP 3 was a major IGFBP in PD as in serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured levels of maternal and cord serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 in 8 White Class B insulin dependent DP and 8 non diabetic pregnancies ( NP ) . ^^^ No significant differences were found between DP and NP with respect to cord serum IGF 2 , maternal serum IGF 1 , IGF 2 , C peptide , IGFBP 1 , IGFBP 2 and IGFBP 3 , and the concentration and affinity of TR for IN , IGF 1 and IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This availability depends on the extent of IGFBP 3 proteolysis ( which promotes release of bound IGF 2 ) and on the proportion of intact forms ( which sequestrate secreted IGF 2 ) . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Cord sera were obtained from term , Chilean newborns exhibiting various patterns of intrauterine growth and assayed for IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 by specific radioimmunoassays ( RIA ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We also measured ultrafiltrated serum free IGF 1 and IGF 2 , total IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and insulin in a subgroup of 18 placebo treated and 20 lanreotide treated patients . ^^^ Lanreotide treatment resulted in minor reductions ( P < . 05 ) in free and total IGF 2 and IGFBP 3 , whereas insulin was unaltered . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In comparison with IGFBP 3 , rh mac 25 has at least a 5 6 fold lower affinity for IGF 1 and 20 25 fold lower affinity for IGF 2 . mac 25 mRNA was detectable in a wide range of normal human tissues , with decreased expression in breast , prostate , colon , and lung cancer cell lines . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Ethnicity affects IGFBP 3 and IGF 2 in normal healthy young adult subjects . ^^^ MEASUREMENTS : Fasting measurements of glucose , insulin , IGF 1 , IGF 2 , IGFBP 1 and IGFBP 3 . ^^^ IGF 2 ( C 707 + / 110 vs A 583 + / 75 micrograms / l ; P < 0 . 0001 ) and IGFBP 3 ( C 5 . 9 + / 1 . 2 vs A 5 . 12 + / 1 . 17 mg / l ; P = 0 . 01 ) levels were significantly higher in Caucasian subjects . ^^^ CONCLUSIONS : Ethnic differences in IGFBP 3 and associated IGF 2 levels may affect the inter relationships of IGFs and their binding proteins and need to be considered when interpreting IGF data on growth and metabolism . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum profiles of GH , IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , insulin , glucose and non esterified fatty acids ( NEFA ) were recorded during the studies . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Factors contributing to the defect are : ( 1 ) low ALS levels , secondary to suppressed growth hormone ( GH ) ; ( 2 ) defective IGFBP 3 binding to ALS ; ( 3 ) reduced ability of pro IGF 2 forms to complex normally , and ( 4 ) very high levels of other IGFBPs , including IGFBP 2 and IGFBP 6 , which might limit the formation of complexes with IGFBP 3 . ^^^ While both GH and glucocorticoids can restore normoglycemia and increase high molecular weight IGFBP 3 complexes , corticosteroid treatment suppresses tumor IGF 2 , whereas GH can restore normoglycemia despite continuing high IGF 2 levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Genetic and environmental components of interindividual variation in circulating levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 . ^^^ We assessed the magnitude of the genetic component in the variation of circulating levels of insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) , and their binding proteins IGFBP 1 and IGFBP 3 by measuring their serum concentrations in 32 monozygotic and 47 dizygotic adult twin pairs of the same sex . ^^^ Women had higher IGF 2 levels than men ( 635+ / 175 vs . 522+ / 144 microg / liter ; P < 0 . 0001 ) and IGFBP 3 levels were also higher in women compared with men ( 5441+ / 1018 vs . 4496+ / 1084 microg / liter ; P < 0 . 001 ) . ^^^ The proportion of variance attributable to genetic effects was 38 % for the IGF 1 concentration , 66 % for the IGF 2 concentration , and 60 % for the IGFBP 3 concentration . ^^^ Our results show that , in adults , there is a substantial genetic contribution responsible for interindividual variation of the circulating levels of IGF 1 , IGF 2 , and IGFBP 3 , but not for the IGFBP 1 levels . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The co ordinate maximum expression of mRNA for both IGF 1 and IGF 2 , the type 1 IGF receptor and IGFBP 3 during the period when the gametes and embryo are in transit suggests a role for IGF 1 and possibly IGF 2 peptides in providing an oviductal environment propitious to conception and early embryonic growth and metabolism . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To investigate the contribution of the insulin like growth factors ( IGFs ) and their binding proteins ( IGFBPs ) to the regulation of bone growth in 10 GH deficient Japanese children receiving recombinant GH therapy , we determined the percent increase from pretreatment levels of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , and bone specific alkaline phosphatase isoenzyme ( B ALP ) . ^^^ The elevation of IGF 1 induced by GH was significantly correlated with the increases in IGFBP 3 ( r = 0 . 735 ; P < 0 . 0001 ) and IGFBP 5 ( r = 0 . 795 ; P < 0 . 0001 ) , and the elevation of B ALP was also significantly positively correlated with the increases in IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 ( r = 0 . 544 , P < 0 . 0001 ; r = 0 . 268 , P = 0 . 0399 ; r = 0 . 414 , P = 0 . 0010 ; and r = 0 . 500 , P < 0 . 0001 , respectively ) . ^^^ Our data are consistent with the anabolic effect on bone growth of GH treatment being mediated by IGF 2 , IGFBP 3 , and IGFBP 5 as well as by IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The effects of treatment with the aromatase inhibitors aminoglutethimide ( AG ) and formestane or the synthetic progestin megestrol acetate ( MA ) on plasma levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding proteins ( IGFBPs ) , and IGFBP 3 protease status were investigated in 39 patients suffering from advanced breast cancer . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 3 levels were determined at 0 , 3 , 6 , 9 , and 12 months . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : ( 1 ) Compared to 100 normal weight girls of same age the obese subjects had increased levels of IGF 1 , IGFBP 1 , IGFBP 2 , and IGFBP 3 and normal levels of IGF 2 . ( 2 ) After weight loss ( 8 . 1 + / 2 . 0 kg ) IGF 2 and IGFBP 2 increased and IGF 1 , IGFBP 1 , IGFBP 3 and the ratio IGF I / IGFBP 3 decreased significantly . ( 3 ) Correlation analysis revealed that fasting insulin levels of the patients were positively correlated with IGF 1 but inversely with IGFBP 1 and IGFBP 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Treatment with 5 50 microM phenytoin reproducibly and markedly increased ( up to 6 fold , p < 0 . 001 ) the mRNA of transforming growth factor ( TGF ) beta 1 , but not that of other growth factors ( i . e . , IGF 2 , platelet derived growth factor A [ PDGF A ] , PDGF B , and TGF beta 2 ) and IGF binding proteins ( i . e . , IGFBP 3 , 4 , and 5 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Exogenous IGF 1 , IGF 2 , and an IGF 1 analog that binds poorly to IGF binding protein 3 ( des IGF 1 ) increased DNA synthesis of HL 60 and U 937 cells in a dose dependent ( 1 25 ng / ml ) fashion by 2 to 4 fold in serum free medium , whereas KG 1 cells did not respond to any of these growth factors . ^^^ The IGF induced increase in proliferation of HL 60 promyeloid cells was inhibited by soluble IGF binding protein 3 ( 500 ng / ml ) when these cells were stimulated with 10 ng / ml of either IGF 1 ( 53 + / 8 % ) or IGF 2 ( 59 + / 8 % ) , but not with des IGF 1 ( 3 + / 1 % ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report maternal serum levels of IGF 1 , IGF 2 , and IGFBP 1 , 2 , and 3 ; IGFBP 1 phosphorylation ; and IGFBP 3 proteolysis in pregnancies ( n = 139 ) complicated by trisomy 18 or trisomy 21 compared with those in normal controls . ^^^ Maternal IGF 1 , IGF 2 , and IGFBP 3 showed no significant difference between fetuses with a normal karyotype and those with trisomy 18 or 21 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study investigated the expression of the mRNA encoding the insulin like growth factors ( IGF ) and IGF binding proteins ( IGFBP ) and have demonstrated IGF 2 , IGFBP 2 , and IGFBP 3 to be altered . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In this study significantly higher concentrations of insulin like growth factor binding protein 3 ( IGFBP 3 ) , insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) and transforming growth factor beta 2 ( TGF beta 2 ) were found in the Na / K > 3 sub group . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The affinity of this highly phosphorylated IGFBP 1 was compared with that of nonphosphorylated IGFBP 1 and recombinant human ( rh ) IGFBP 3 by equilibrium binding to IGF 2 and IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum Insulin like growth factor 2 ( IGF 2 ) levels in patients with acromegaly and growth hormone deficiency : correlation with IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To test this hypothesis , conditioned media ( CM ) from normal human bone cells ( control and IGF 2 treated 48 h cultures ) from five different skeletal sites were obtained and assayed for IGF 1 , IGF 2 ( following separation of IGF binding proteins [ IGFBPs ] ) , IGFBP 3 , IGFBP 4 , and IGFBP 5 protein levels employing specific radioimmunoassays for each protein . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 had no effect on alkaline phosphatase ( ALP ) activity of E 1 cells , but in the presence of IGF 2 at an ineffective concentration stimulated ALP activity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGF 1 and IGF 2 ) and IGF binding protein 3 production by fibroblasts of patients with Turner ' s syndrome in culture . ^^^ We compared the ability of fibroblasts from six TS patients with that of fibroblasts from six age matched control ( C ) subjects to synthesize in vitro IGF 1 , IGF 2 , and IGFBP 3 under basal and GH , estradiol ( E 2 ) , or GH plus E 2 stimulated conditions . ^^^ Furthermore , we evaluated IGF 1 , IGF 2 , and IGFBP 3 messenger ribonucleic acid ( mRNA ) expression in fibroblasts from TS and C subjects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 1 and IGFBP 3 , but not an N terminal fragment of IGFBP 3 , could effectively block binding of both IGF 1 and IGF 2 to the solid phase IGFBP 3 . ^^^ Possibly , the interaction of IGFBP 3 with heparin or heparin like structures in vivo can lead to the selective release of IGF 2 from this binding protein . ^^^ Our results with heparin also suggest that the binding sites on IGFBP 3 for IGF 1 and IGF 2 are not completely identical . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^ Serum IGF 2 but not IGF 1 or IGFBP 3 was higher in PC and BPH than in controls ( PC 332 + / 23 micrograms / l ) , BPH 359 + / 26 micrograms / l vs controls 241 + / 37 micrograms / l ; P = 0 . 03 and 0 . 02 respectively ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Following a five day maternal fast , both IGF 1 and IGF 2 levels were decreased in the maternal and fetal circulation ( P < 0 . 05 ) , accompanied by a decrease in fetal hepatic IGF 1 mRNA levels , but the IGFBP 2 level was increased and the IGFBP 3 level was decreased in maternal circulation , whereas the IGFBP 1 level was increased in fetal circulation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum PSA ( by EIA ) , and IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 ( by RIA ) were measured in all subjects , and serially in patients with PC . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To assess the role of insulin like growth factors ( IGFs ) in growth and transformation of normal ( myometrium ) and tumorous smooth muscle cell ( SMC ) tissues , in situ hybridization ( ISH ) analysis for insulin like growth factor 1 and 2 ( IGF 1 and IGF 2 ) mRNAs was combined with detection of IGF peptides , their receptors and IGF binding protein 3 ( IGFBP 3 ) . mRNAs for both IGFs were detected in smooth muscle cells in normal , benign and malignant SMC tissues , together with the IGF peptides , both IGF receptors and IGFBP 3 . ^^^ Low grade leiomyosarcomas contained more IGF 2 mRNAs than myometrium and leiomyoma , fewer type 2 IGF / mannose 6 phosphate receptors and less IGFBP 3 than myometrium and , in addition , fewer IGF 1 mRNAs and type 1 IGF receptors than leiomyoma . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
After 12 months of study , the 30 children receiving rhGH showed : ( 1 ) greater increase in height ( 9 . 1 + / 2 . 8 vs . 5 . 5 + / 1 . 9 cm , P < 0 . 0001 ) ; ( 2 ) increases in serum levels of IGF 1 , IGF 2 , free IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) ; ( 3 ) a greater decrease in serum IGFBP 1 levels ; and ( 4 ) no significant difference in serum IGFBP 2 levels , when compared to the 14 control patients . ^^^ The change in height SDS after 12 months of rhGH ( +0 . 8 ) in the 30 treated children correlated significantly and positively with serum ALS , IGFBP 3 , total IGF , IGF 1 , IGF 2 and free IGF 1 levels measured during treatment . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Immunological determination of IGFBP 2 , IGFBP 3 , IGF 1 , IGF 2 , interleukin 1 beta ( IL 1 beta ) and tumour necrosis factor alpha ( TNF alpha ) was undertaken in the SF and plasma of 115 patients with rheumatoid arthritis ( RA ; n = 53 ) , osteoarthritis ( OA ; n = 44 ) and other rheumatic disorders . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Spontaneous GH secretion , IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , their binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) values at the time of clinical diagnosis ( n = 65 ) , after a 25 % decrease in the body mass index ( BMI ) expressed as the SD score ( BMI SD score ; n = 29 ) , and after a diminution of at least 50 % of the initial BMI SD score ( n = 9 ) are reported . ^^^ Serum IGFBP 3 levels correlated positively with IGF 1 , fIGF 1 , and IGF 2 in all groups , but these correlations were weaker in the obese patients at diagnosis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report their spontaneous GH secretion and IGF 1 , free IGF 1 ( fIGF 1 ) , IGF 2 , the IGF binding proteins ( IGFBP 1 , IGFBP 2 , and IGFBP 3 ) , and GH binding protein ( GHBP ) levels at the time of the clinical diagnosis ( n = 50 ) and after recuperation of between 6 8 % ( n = 42 ) and 10 % or less of the initial weight ( n = 20 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Fasting levels of IGF 1 , free IGF 1 , IGF 2 , IGFBP 3 , and insulin increased significantly within 3 months of GH treatment , whereas IGFBP 1 , IGFBP 2 , and IGFBP 6 showed no change . ^^^ IGFBP 3 and IGF 2 both showed late ( > 20 h ) responses to a dose of GH , whereas IGFBP 2 and IGFBP 6 showed minimal changes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Immunostaining of sections of normal and cancer tissue was negative for IGF 1 and IGF 2 ; IGFBP 2 was positive in 2 out of 10 cancer tissues and 7 out of 10 normal tissues ; IGFBP 3 was positive in 7 out of 10 cancer tissues and 7 out of 10 normal tissues ; and IGFBP 4 was positive in 5 out of 10 cancer tissues and 6 out of 10 normal tissues . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Circulating concentrations of fetal , but not maternal , IGF 1 increased with treatment ( approximately 80 to approximately 1015 ng / ml ) , and there was no evidence of a change in serum IGF 2 or an increase in IGF binding protein 3 compared with historical control values . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Children with CRF who are treated with GH show catch up growth that correlates positively with the increase in each component of the 150 kd serum ternary complex ( acid labile subunit , IGFBP 3 , IGF 1 , and IGF 2 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The samples were assayed for IGF 1 , IGF 2 , IGF binding protein 3 ( IGF BP 3 ) , GH and estradiol . ^^^ There was no significant change in the levels of either IGF 2 or IGF BP 3 in any of the groups . ^^^ We can conclude that ( 1 ) there is a negative correlation between GH and IGF 1 plasma levels in patients undergoing controlled ovarian hyperstimulation ( COH ) IVF , when levels of estradiol and GH are elevated ; ( 2 ) plasma levels of IGF 1 under ovarian hyperstimulation are probably regulated by a multifactorial system ; and ( 3 ) no correlation was found between the plasma levels of IGF 1 and those of IGF 2 and IGF BP 3 in all patient groups . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In conclusion , the data point to IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 as putative mediators of relaxin induced uterine growth in the pig . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Compared with native IGFBP 3 , pure IGFBP 3 ( 29 ) has a 4 10 fold lower affinity for IGF 2 and a 200 fold lower affinity for IGF 1 . ^^^ Consistent with the binding data , IGFBP 3 ( 29 ) inhibited IGF 2 stimulated thymidine incorporation in chondrosarcoma cells , but was a less potent inhibitor than native IGFBP 3 ; also , native IGFBP 3 clearly inhibited IGF 1 stimulated thymidine incorporation in chondrosarcoma cells and potentiated IGF 1 stimulated aminoisobutyric acid uptake in bovine fibroblasts , but higher concentrations of IGFBP 3 ( 29 ) had no effect on these IGF 1 actions . ^^^ Thus , the 29 kD IGFBP 3 form that accumulates in CRF serum and extravascular spaces is an IGFBP 3 fragment that may modulate IGF 2 , but not IGF 1 , effects on target tissues . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the present study , men with idiopathic osteoporosis were compared with healthy men , with respect to bone histomorphometry and to serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 and IGFBP 3 , and 24 h urinary excretion of GH . ^^^ In the patients , serum concentrations of IGFBP 3 were reduced , compared with controls , with a 46 % lower mean value ; whereas levels of IGF 1 , IGF 2 , IGFBP 2 , and GH were similar in the two groups . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 were elevated 3 to 13 fold in nondiabetic retinal ischemia and 1 . 5 to 3 fold in PDR , indicating that the changes were not restricted to diabetes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Binding sites and binding properties of binary and ternary complexes of insulin like growth factor 2 ( IGF 2 ) , IGF binding protein 3 , and acid labile subunit . ^^^ It was found that not only the IGFBP 3 derivatives with the amino terminal , cysteine rich domain ( N domain ) but also the synthetic peptide maintained affinity for IGF 2 . ^^^ These results strongly suggest that : 1 ) the N domain , containing at least Glu 52 Ala92 , of rat IGFBP 3 is important for binding to IGF 2 ; 2 ) the C domain of IGFBP 3 is essential for binding to the acid labile subunit both in the presence and absence of IGF 2 ; and 3 ) Tyr 27 of IGF 2 is important for the ternary complex formation . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Both human and rat mesangial cells grown on BSA AGE showed increased IGF 1 and total and bioactive TGF beta medium levels and enhanced IGF 1 , IGF 2 , and TGF beta 1 gene expression , compared with cells grown on BSA , whereas total IGFBP and IGFBP 3 medium content , IGF receptor density and affinity , and IGF 1 receptor transcripts were unchanged . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Human trabecular osteoblasts produce mainly IGF 2 , IGFBP 3 and minute quantities of IGF 1 in culture . ^^^ GH does not seem to regulate the local production of IGF 2 or IGFBP 3 . ^^^ No differences in the basal or GH stimulated production of IGF 1 , IGF 2 or IGFBP 3 were found between osteoporotic patients and age matched normals . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factors ( IGF 1 and IGF 2 ) and their binding protein ( IGFBP 3 ) are not elevated in pancreatic cancer . ^^^ CONCLUSION : Serum levels of IGF 1 , IGF 2 , and IGFBP 3 are not elevated in pancreatic cancer and do not appear to have a significant role in glucose homeostasis in this group of patients . ^^^ METHODS : Serum levels of IGF 1 , IGF 2 , and IGFBP 3 were measured by RIA in 20 patients with pancreatic cancer and 20 age matched healthy control subjects and correlated with serum glucose , C peptide , and glucose tolerance . ^^^ RESULTS : No significant difference was observed in serum levels of IGF 1 ( 13 vs 17 nmol / L , respectively ) , IGF 2 ( 0 . 67 vs 0 . 91 U / mL ) , or IGFBP 3 ( 2 . 3 vs 2 . 3 mg / L ) between the two groups of patients . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Before weaning , plasma IGF 1 concentrations were higher in rams than ewes , and plasma concentrations of IGF 2 and IGF binding protein 3 ( IGFBP 3 ) also tended to be higher in rams than in ewes . ^^^ Plasma concentrations of IGF 1 and IGFBP 3 did not differ between rams and ewes at 100 days of age , but plasma IGF 2 was higher in rams than in ewes at this time . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Ligand blot analysis revealed a significant increase in IGF 2 binding to a 29 31 kilodalton region where positive staining with specific antibodies to IGFBP 3 or IGFBP 1 was observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The mRNA for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , in dominant and subordinate follicles were measured to determine if changes in IGF or IGFBP gene expression are associated with follicular dominance . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
PATIENTS AND METHODS : Circulating concentrations of leptin , IGF 1 , IGF 2 , and insulin like growth factor binding protein 3 ( IGFBP 3 ) were measured in a population based sample of 50 to 80 year old men ( n=217 ) and women ( n=198 ) by specific RIA . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As hypercholesterolemia is common in GH deficient adults , and because there is experimental evidence that GH may play a role in regulating plasma cholesterol , we decided to investigate the activity of the GH IGF axis in an elderly population by measuring serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) levels and to study their relationship with blood lipid levels . ^^^ Men had significantly lower levels of IGFBP 3 , high density lipoprotein cholesterol ( HDL C ) and apoprotein A 1 ( ApoA 1 ) compared to the women , whereas IGF 1 and IGF 2 were only slightly lower . ^^^ Using linear regression analysis , we observed an inverse relationship of age with IGF 1 ( r = 0 . 35 ; P < 0 . 001 ) , IGF 2 ( r = 0 . 40 ; P < 0 . 001 ) , IGFBP 3 ( r = 0 . 52 ; P < 0 . 001 ) , body mass index , and lipid levels . ^^^ Partial correlation analysis , after adjustment for age and body mass index , showed that IGFBP 3 and IGF 2 were still significantly and positively related to HDL C and ApoA 1 . ^^^ Furthermore , a strong association was documented among IGF 1 , IGF 2 , and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 and IGF 2 were quantified by radioimmunoassay ( RIA ) , IGFBP 3 by immunoradiometric assay ( IRMA ) , and changes in IGFBP 1 , 2 , 3 , and 4 were estimated by Western ligand blotting ( WLB ) . ^^^ The decreases observed in IGF 1 , IGF 2 , and IGFBP 3 in patients with hepatic failure and their subsequent restoration after LT probably result primarily from the reduced number of functional hepatocytes in ESLD and their subsequent replacement by healthy hepatic tissue . ^^^ We conclude that LT in patients with severe hepatic insufficiency enhances the potential for normal cell growth and replication by restoring serum IGF 1 , IGF 2 , and IGFBP 3 concentrations to normal concomitantly with the improvement in hormonal and nutritional status . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The Objective of this paper is to determine the relation between fetal macrosomia in offspring of nondiabetic women , and the levels of insulin like growth factors ( IGF 1 , IGF 2 ) , insulin growth factor binding protein 3 ( IGFBP 3 ) and insulin , in maternal and neonatal compartments . ^^^ Maternal and neonatal serum samples were analyzed for levels of IGF 1 , IGF 2 , IGFBP 3 and insulin , by specific radioimmunoassays ( RIAs ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The aim of our study was to determine the serum levels of IGF 1 , IGF 2 , and their binding proteins IGFBP 1 and IGFBP 3 in young children 3 months to 2 years of age with nonorganic failure to thrive ( NOFTT ) . 29 children with NOFTT and 32 normal controls were recruited and studied for auxologic and hormonal parameters . ^^^ IGF 1 and IGF 2 serum levels were highly correlated to serum IGFBP 3 but there were no differences in the serum IGF 1 , IGF 2 and IGFBP 3 levels between the groups . ^^^ We conclude that IGF 1 , IGF 2 , and IGFBP 3 are not useful for the evaluation of nutritional status in children , under the age of 2 years , with NOFTT . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Analysis of the distribution of IGF 1 , IGF 2 and IGFBP 3 in fluid from healthy and atretic follicles revealed that the majority of these growth factors ( > 80 % of total IGF 2 ) were in the 150KDa complex , indicating that the ALS present was functional , in that it formed the ternary complex with a molecule of IGFBP 3 and IGF . ^^^ No free IGF 2 was found in any of the follicular fluids analysed nor was there any increase in the amount of unsaturated IGFBP 3 in atretic follicles . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As observed for IGFBP 3 , ternary complexes containing IGFBP 5 form preferentially in the presence of IGF 1 , even though IGFBP 5 has a preferential affinity for IGF 2 over IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
By studying the basis of diabetic abnormalities relating to the growth hormone ( GH ) , the insulin like growth factor ( IGF ) dynamics in this patient , i . e . reductions of GH , IGF 1 , IGF 2 , IGF Binding protein ( IGF BP ) 2 and IGF BP 3 , were detected . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The mouse C 2 myoblast cell line stably transfected with a vector producing IGF 2 antisense RNA was used to show that specific IGFBP expression changes with the state of the cells : high levels of IGFBP 2 messenger RNA ( mRNA ) were found only in proliferating myoblasts , whereas IGFBP 3 mRNA was induced in quiescent cells . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
GH and IGF 1 basal concentrations were significantly higher in pre menopausal than in post menopausal women , while IGF 2 , IGFBP 1 and IGFBP 3 concentrations did not vary significantly . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , estradiol and progesterone were measured by radioimmunoassay ( RIA ) in each sample . ^^^ No change in plasma levels of IGFBP 1 and IGF 2 was found and immunoblots did not reveal any alteration in the ratio of fragmented to intact IGFBP 3 during the menstrual cycle . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The major cell associated binding site for [ 125I ] IGF 1 is IGFBP 3 and for [ 125I ] IGF 2 are IGFBP 3 and IGFBP 5 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Northern blots were performed using total RNA hybridized with 32P labeled cDNA probes ( human IGF 1 and human IGFI R ) and cRNA probes ( rat IGF 2 , human IGFII R , human IGFBP 1 , pig IGFBP 3 , and pig GHR ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 levels were increased during the PO phase ( 253 + / 20 mg / L ) compared to those during the EF phase ( 210 + / 16 mg / L ; P = 0 . 03 ) , whereas serum IGF binding protein 3 , IGF 2 , and GH binding protein were similar during the two phases . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The effects of recombinant human IGF 1 administration on concentrations of acid labile subunit , IGF binding protein 3 , IGF 1 , IGF 2 and proteolysis of IGF binding protein 3 in adolescents with insulin dependent diabetes mellitus . ^^^ We have therefore studied the effects of a single subcutaneous dose of rhIGF 1 ( 40 micrograms / kg at 1800 h ) , when compared with an untreated control night , in 17 subjects with IDDM , on serum concentrations of IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and IGFBP 3 proteolysis . ^^^ On the baseline night , IGFBP 3 levels correlated with the sum of IGF 1 and IGF 2 ( r = 0 . 73 , P = 0 . 02 ) and with levels of the ALS ( r = 0 . 7 , P = 0 . 002 ) . ^^^ However after rhIGF 1 , the sum of IGF 1 and IGF 2 no longer correlated with IGFBP 3 , whereas the relationship with ALS was maintained . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In this study , testosterone , dihydrotestosterone ( DHT ) , 3 alpha androstanediol ( 3 alpha diol ) , IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 concentrations were evaluated in human benign prostatic hyperplasia ( BPH ) tissue . ^^^ IGF 1 , IGFBP 2 , and IGFBP 3 were measured by radioimmunoassay , and IGF 2 by immunoradio metric assay , after acidification and chromatography on Sep pak C 18 Cartridges for IGF 1 and IGF 2 . ^^^ IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels were 24 + / 3 . 7 , 121 + / 14 ng / g tissue and 0 . 44 + / 0 . 05 and 1 . 2 + / 0 . 17 micrograms / g tissue , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We demonstrate that the 1 , 25 ( OH ) 2D3 and its analog EB 1089 induced growth inhibition was associated with increased IGFBP 3 mRNA abundance , IGFBP 3 mRNA stability , IGFBP 3 protein accumulation , and decreased IGF 2 gene expression . ^^^ Anti IGF 2 antibody and exogenous recombinant human IGFBP 3 inhibit PC 3 cell proliferation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured serum levels of free IGF 1 and free IGF 2 , total IGF 1 , total IGF 2 , big IGF 2 and IGFBP 1 , IGFBP 2 and IGFBP 3 in patients with NICTH before ( n=14 ) and after surgical removal of the tumour ( n=3 ) . ^^^ In accordance with earlier observations levels of total IGF 1 , total IGF 2 , and IGFBP 3 were decreased , whereas IGFBP 1 and IGFBP 2 were increased in NICTH ( all p values < 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied 11 elderly male ( 50 78 years ) marathon runners and 10 age matched male ( 52 73 years ) sedentary controls to evaluate plasma concentrations of GH , total and free IGF 1 and IGF 2 and of IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 and insulin . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The amounts of IGFBP 3 in conditioned media were relatively low under basal conditions when analyzed by ligand blotting with 125I IGF 2 , but were increased dramatically relative to serum free controls by exposure to IGF 1 ( 100 ng / ml ) or IGF 2 ( 100 ng / ml ) for 24 h . ^^^ These increases in IGFBP 3 protein corresponded with dose dependent increases in IGFBP 3 mRNA , with IGF 2 eliciting a smaller response than was elicited by IGF 1 at each concentration . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To determine the effects of hMG and highly purified FSH on follicular production of the ovarian growth factors transforming growth factor beta 1 ( TGF beta 1 ) , insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) , and insulin like growth factor binding proteins 1 and 3 ( IGFBP 1 and IGFBP 3 ) . ^^^ IGF 2 , IGFBP 1 and IGFBP 3 in follicular fluid and levels of E 2 in serum were measured . ^^^ Levels of IGF 2 and IGFBP 3 were similar with the 2 treatments . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Free IGF 1 and IGF 2 were measured by ultrafiltration of serum , and IGFBP 3 proteolytic activity was determined by a [ 125I ] recombinant human IGFBP 3 degradation assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
All subjects underwent a 24 h GH profile ( 20 minute sampling ) , measurement of serum IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and growth hormone binding protein ( GHBP ) and , after an overnight fast , an AST ( intravenous arginine 20 g / m2 over 30 minutes ) . ^^^ Normative data for serum IGF 1 , IGF 2 , IGFBP 3 and IGFBP 2 were obtained from 125 subjects aged 60 87 years . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 and IGFBP 3 concentrations correlated with body weight , thus reflecting breed size as previously shown , whereas IGF 2 concentrations did not . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Concomitant with IGFBP 2 expression in these tumours , were a decrease in IGFBP 1 expression and an increase in IGFBP 3 proteolysis , both of which increase the bioavailability of the IGF 2 produced by the cells . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the present study , using Western ligand and immunoblotting methods , we investigated IGF binding proteins ( IGFBPs ) , IGFBP 3 proteolysis and big IGF 2 in pre and postoperative serum from two patients with EPTH due to benign pleural fibroma . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma levels of insulin like growth factor ( IGF ) 1 , IGF 2 and IGF binding protein 3 in the evaluation of childhood growth hormone deficiency . ^^^ METHODS : Serum IGF 1 , IGF 2 , IGFBP 3 and various molar ratios were , after correction for age and sex using SD scores , compared to the maximum serum GH peak after two standard provocation tests using four different methods ( t test , chi 2 , likelihood ratios and ROC curves ) . ^^^ RESULTS : IGF 1 performed better than IGFBP 3 , but the best results were achieved by the molar ratio IGF 1 : IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 1 was reduced ( 216 ng / mL ) , although still in the high range , whereas IGF 1 ( 71 ng / mL ) , IGFBP 3 ( 0 . 62 microg / mL ) , and IGF 2 ( 111 ng / mL ) were only slightly increased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF effects are mediated by IGFBP and receptor interactions , hence dietary induced changes in intestinal IGF 2 receptor patterns and IGFBP 3 message levels were investigated . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have therefore investigated the effects of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) and intact IGFBP 3 on in vitro sperm motility . ^^^ Washed motile sperm selected by the ' swim up ' method , from normozoospermic samples , were incubated at 37 degrees C in 5 % CO 2 in air with IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , or control Earle ' s media , and were examined at time 0 and after 60 min incubation . ^^^ In contrast , IGF 2 , IGFBP 2 , and a combination of IGF I / IGFBP 3 , had no significant effects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The use of recombinant human ( rh ) GH has been reported to alleviate hypoglycaemia in non islet cell tumour hypoglycaemia , and the mechanism is thought to relate to GH mediated increments in serum levels of IGF binding protein 3 ( IGFBP 3 ) , thereby reducing the bioavailability of IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
PURPOSE : Follicular fluid concentrations of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein ( BP ) 1 , and IGFBP 3 in 57 women undergoing in vitro fertilization and embryo transfer were examined to determine whether levels reflected differences in patients ' exposure to gonadotropin stimulation and a diminished ovarian reserve . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We evaluated the GH bioactivity ( GH BIO ) , GH serum level by immunofluorimetric assay ( GH IFMA ) , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 in 21 prepubertal obese children ( 13 boys and eight girls ) aged 5 . 7 to 9 . 4 years affected by simple obesity and in 32 ( 22 boys and 10 girls ) age and sex matched normal weight controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Other results were : IGF 1 , 3 11 ng / mL ( normal , 114 492 ) ; IGF 2 , 185 299 ng / mL ( normal , 358 854 ) ; IGF binding protein 1 ( IGFBP 1 ) , 12 200 ng / mL ( normal , 13 73 ) ; IGFBP 2 , 60 384 ng / mL ( normal , 55 480 ) ; and IGFBP 3 , 400 600 ng / mL ( normal , 2000 4000 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGFBP 5 , and total protein were determined in extracts obtained after ethylenediamine tetraacetate and guanidine hydrochloride extraction . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To examine the possible causal role of IGFs in lung cancer development , we compared plasma levels of IGF 1 , IGF 2 , and an IGF binding protein ( IGFBP 3 ) in patients with newly diagnosed lung cancer and in control subjects . ^^^ IGF 1 , IGF 2 , and IGFBP 3 plasma levels were measured by enzyme linked immunosorbent assay and then divided into quartiles , based on their distribution in the control subjects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Treating CRF children with GH for 12 months increased serum IGFBP 4 levels by 26 % and IGFBP 5 levels by 49 % , as determined by RIA ; levels of IGFBP 5 , but not IGFBP 4 , correlated significantly with serum levels of IGF 1 , IGF 2 , IGFBP 3 , and acid labile subunit and with growth rate in these GH treated children . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These observations suggest the possibility of an autocrine IGF 2 loop that is regulated by the relative expression of IGF 2 , IGFBP 3 , and IGFBP 6 , and IGFBP proteases in these keratinocytes , although demonstration of this loop requires further study . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Chronic liver disease was characterised by low serum IGF 1 , IGF 2 , IGFBP 3 and ALS levels with raised IGFBP 1 and 2 levels . ^^^ After OLT , there was a rapid normalisation of serum IGF 1 , while IGF 2 and IGFBP 3 overshot to supranormal levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Since no single factor acts in isolation , this study was performed to assess the influence of oestradiol ( total and unbound ) , insulin , insulin like growth factor ( IGF ) 1 , IGF 2 and the binding proteins IGFBP 1 , and IGFBP 3 , on basal and adrenocorticotrophic hormone ( ACTH ) stimulated adrenal androgen secretion in 25 women with PCOS and 10 matched ovulatory controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Leptin , insulin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 were measured by specific immunoassays . ^^^ There were also significant increments in insulin , IGF 1 , and IGFBP 3 , whereas IGF 2 , IGFBP 1 , and IGFBP 2 did not change over 9 months of GH treatment . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In AN , the levels of GH binding protein , free and total IGF 1 , free IGF 2 , and IGFBP 3 were significantly reduced ; total IGF 2 , IGFBP 2 , and IGFBP 4 levels were unchanged ; and IGFBP 1 was increased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Furthermore , the increase in serum IGF 1 , IGF 2 , and triiodothyronine correlated with the increase in IGFBP 3 . ^^^ CONCLUSION : GH significantly increased serum IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 4 and decreased serum IGFBP 1 and IGFBP 2 in post menopausal women . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
At switch from CaCo 2 cell proliferation to differentiation a marked increase in the secretion of IGF 2 ( 10 fold ) , IGFBP 1 ( 2 . 5 fold ) , IGFBP 2 ( 3 fold ) , and IGFBP 3 ( 6 fold ) was measured . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In contrast , IGF 1 or IGF 2 ( 6 . 5 nM ) preferentially induced the accumulation of IGFBP 3 ( 1 . 9 fold ) and to a lesser extent of IGFBP 4 , but did not show any effect on IGFBP 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Alteration in pancreatic immunoreactivity of insulin like growth factor ( IGF ) binding protein ( IGFBP ) 6 and in intracellular degradation of IGFBP 3 in fibroblasts of IGF 2 receptor / IGF 2 deficient mice . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Fasting ALS , IGF 2 , IGFBP 3 , IGFBP 2 , IGFBP 1 , and insulin levels before rhIGF 1 treatment were 46 . 3 mg / L , 1044 microg / L , 5 . 8 mg / L , 73 ng / mL , 4 . 7 ng / mL , and 27 . 3 mU / L , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
High blood levels of an abnormal form of insulin like growth factor type 2 ( IGF 2 ) associated with low blood levels of insulin , growth hormone , IGF 1 , and IGF BP 3 were observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 ( Ad ) had similar affinities for IGF 1 and ALS and an approximately 25 % decreased affinity for IGF 2 compared to IGFBP 3 ( Pl ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Pigs were bled on Days 4 , 13 , 22 , and 31 of lactation and the plasma was analyzed for insulin like growth factor ( IGF ) 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) . ^^^ In conclusion , pST had no little or no effect on growth performance or plasma IGF 1 , IGF 2 , or IGFBP 3 in sucking pigs on either a high or low plane of nutrition . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To examine whether insulinlike growth factor 1 ( IGF 1 ) , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) in serum and peritoneal fluid ( PF ) correlate with the presence and severity of endometriosis . ^^^ Simultaneous sampling of blood and PF was performed during laparoscopy , and IGF 1 , IGF 2 and IGFBP 3 levels were determined by immunoradiometric assay . ^^^ There were no significant differences in IGF 2 and IGFBP 3 levels among the groups . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN : Cross sectional analysis of the relationship between insulin resistance syndrome variables and concentrations of IGF 1 , IGF 2 , IGFBP 1 and IGFBP 3 in 80 subjects with NIDDM . ^^^ There were no significant relationships between levels of IGF 2 and any of the variables investigated , and IGFBP 3 levels only correlated with those of total cholesterol ( r = 0 . 24 , P = 0 . 04 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The concentrations measured by RIA on d 3 were 10 . 7+ / 0 . 4 ng / mL for IGF 2 , 1 . 9+ / 0 . 1 ng / mL for IGF 1 , 100+ / 5 ng / mL for IGF binding protein 3 ( IGFBP 3 ) , and 2163+ / 108 ng / mL for IGFBP 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study used a cross sectional design to investigate relationships among serum insulin like growth factor ( IGF ) parameters ( total serum IGF 1 , IGF 2 , and IGF binding protein 3 ) , serum estradiol , and bone mineral density ( BMD ) stratified for potential confounders , and a longitudinal design to investigate the effects of hormonal replacement therapy ( HRT ) on IGFs and BMD . ^^^ In the follow up study , HRT decreased IGF 1 and IGF 2 , but did not influence the age related decline in IGF binding protein 3 significantly . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The orders of k ( a ) for both IGF 1 and IGF 2 are IGFBP 3 > IGFBP 5 > IGFBP 6 > IGFBP 4 > IGFBP 2 > ++ +IGFBP 1 . ^^^ The order of k ( d ) for IGF 1 is IGFBP 6 > IGFBP 5 > IGFBP 4 > IGFBP 3 > IGFBP 2 > ++ +IGFBP 1 and that for IGF 2 is IGFBP 5 > IGFBP 6 > IGFBP 2 > IGFBP 4 > IGFBP 3 > ++ +IGFBP 1 , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The insulin like growth factors IGF 1 and IGF 2 and their major binding protein IGFBP 3 influence the growth of breast cancer cells in vitro . ^^^ METHODS : Serum IGF 1 , IGF 2 and IGFBP 3 were measured by specific radioassay in 12 women with benign breast disease , 31 patients with breast cancer and in age matched controls . ^^^ However , the index of free unbound IGF measured as the molar ratio of IGF 1 plus IGF 2 divided by IGFBP 3 was significantly lower in benign breast disease than in the other subjects . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 complexes measured by ELISA 1 and 2 in samples from normal individuals and subjects with GH deficiency , acromegaly , and GH receptor deficiency more tightly correlated with IGF 1 , IGFBP 3 , and ALS than IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 and IGF BP 3 were determined in the serum from 41 patients with colorectal cancer and 50 healthy controls ; data were analyzed using unconditional multiple logistic regression , adjusting for age , gender , education , height and BMI , as well as mutually . ^^^ Both IGF 1 and IGF 2 were positively , while IGF BP 3 was inversely , associated with risk for colorectal cancer , though none of these relations reached statistical significance . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As a consequence of reduced IGFBP 3 proteolysis and elevated IGFBP 3 protein levels , dissociable IGF 2 levels were significantly lower in TIMP 1 overexpressing animals . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have , therefore , determined the concentrations of plasma IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in boars , barrows , and gilts and related these to postnatal growth characteristics . ^^^ Between 15 and 23 wk . of age , IGF 1 and IGFBP 3 , but not IGF 2 , were positively associated with growth rate , voluntary feed intake , and gain : feed ratio . ^^^ Plasma IGF 2 , but not IGF 1 or IGFBP 3 , was positively associated with backfat depth during this period . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We demonstrate that insulin , insulin like growth factor ( IGF ) 1 , and IGF 2 induce expression of HIF 1alpha , which is required for expression of genes encoding IGF 2 , IGF binding protein ( IGFBP ) 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To assess the relative efficacy of hGH and glucocorticoids in the treatment of non islet cell tumour hypoglycaemia ( NICTH ) by examination of their influence on the composition of the various molecular species involving tumour and mature forms of IGF 2 in association with IGFBP 3 . ^^^ Neutral size exclusion chromatography was used in the separation of molecular species before assay for immunoreactive IGF 2 and IGFBP 3 content . ^^^ RESULTS : High dose hGH treatment produced increases in serum levels of big and mature IGF 2 and IGFBP 3 but without generation of high molecular weight complexes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : IGFBP 1 and insulin were measured in samples drawn every 4 h over the 24 h period while IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were determined in samples collected at the end of the study . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Tumour derived IGF 2 has a higher than normal molecular weight ( big ' IGF 2 ' ) and an impaired ability to form the normal ternary 150 kD complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ Northern blot analysis of total mRNA isolated from the tumour demonstrated high expression of the IGF 2 gene and abundant 1 . 1 kb IGFBP 6 transcript , while the genes encoding IGFBP 3 , 4 and 5 were only weakly expressed and mRNA of IGFBP 1 , 2 and IGF 1 could not be detected . mRNAs for the IGF type 2 receptor could be easily demonstrated , whereas those for the insulin and IGF type 1 receptor were hardly detectable . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN , PATIENTS AND MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 , osteocalcin ( OST ) , bone specific alkaline phosphatase ( BAP ) , urinary deoxypyridinoline ( DPYD ) and N telopeptide of type 1 collagen ( NTX ) were measured in 31 premenopausal women aged 31 43 and 65 postmenopausal women aged 47 60 : this latter group comprised 30 normal healthy women and 35 osteoporotic women . ^^^ RESULTS : Compared with premenopausal women or normal postmenopausal women , serum IGF 1 and IGFBP 3 levels were significantly lower in osteoporotic postmenopausal women while no significant differences in serum levels of IGF 2 , IGFBP 1 and IGFBP 2 were observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There were no differences between the PCOS subjects and the normoovulatory group for total IGF 1 , IGF 2 , or IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In addition to measuring IGF 1 , IGF 2 and IGFBP 3 , serum IGFBP 1 , 2 , 4 and 5 were measured . ^^^ Serum IGF 1 ( 25 microg / l ) , IGF 2 ( 194 microg / l ) , IGFBP 3 ( 0 . 4 mg / l ) , and IGFBP 5 ( 87 microg / l ) levels were low compared to age matched prepubertal children . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : Compared with controls , estrogen treated monkeys had 2 fold higher SF levels of IGF 1 ( P < 0 . 001 ) , 1 . 7 fold higher IGF 2 ( P < 0 . 006 ) , 5 . 9 fold higher IGFBP 1 ( P < 0 . 02 ) , and 2 . 5 fold higher IGFBP 3 ( P < 0 . 001 ) . ^^^ Estrogen plus progesterone treated monkeys had SF levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 that were intermediate between the levels in the control and estrogen groups , except that the level of IGFBP 3 was significantly greater than that in the control group ( P < 0 . 001 ) . ^^^ Treatment group did not affect the serum levels of IGF 1 and IL 6 , but IGF 2 and IGFBP 3 were increased by 1 . 6 and 1 . 8 fold , respectively , in the estrogen group ( P < 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We therefore measured IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 , and acid labile subunit ( ALS ) in 27 GHD subjects ( aged 5 82 yr ) from an extended kindred in Northeast Brazil with an identical GHRH receptor mutation and in 55 indigenous controls ( aged 5 80 yr ) . ^^^ In the controls , IGF 2 was associated primarily with IGFBP 3 and to a lesser extent with IGFBP 2 , whereas in GHD the reverse was found . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The high nutritional status of pigs in the early phases of growth diminished the postnatal changes in IGF 1 and IGFBP 2 , but not IGF 2 or IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the spleen , IGFBP 6 expression was not altered by IGF 2 overexpression , whereas IGFBP 3 expression was strongly increased . ^^^ We speculate that an increase of IGFBP 3 expression together with changes in expression of other IGFBPs , inhibits IGF 2 stimulated growth in the spleen by an autocrine / paracrine pathway . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , and IGFBP 3 were assayed among 193 men later diagnosed with colorectal cancer during 14 years of follow up and among 318 age and smoking matched control subjects . ^^^ RESULTS : IGFBP 3 levels correlated with IGF 1 levels ( r= . 64 ) and with IGF 2 levels ( r= . 90 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : Non diabetic obese subjects had increased free IGF 1 and 2 , total IGF 2 , IGFBP 3 and GHBP , reduced IGFBP 1 and 2 ( p < 0 . 05 ) , but normal total IGF 1 , when compared to lean subjects . ^^^ Also IGFBP 1 and total IGF 1 were normal in obese Type 2 diabetics , whereas free and total IGF 2 and IGFBP 3 remained elevated to a similar extent as in simple obesity ( p < 0 . 05 ) . ^^^ Many of these abnormalities were unaffected by the concomitant presence of Type 2 diabetes ( total IGF 1 , free and total IGF 2 and IGFBP 3 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 2 and IGF binding protein 3 ( IGFBP 3 ) were also decreased in GHD without any gender related differences . ^^^ The diagnostic sensitivity ( % ) of IGF 1 , IGF I / GHBP , IGF I / IGFBP 3 , and of the combination of IGF 1 plus IGF 2 ( both low or one normal and one low ) , was higher in female patients than in male ( IGF 1 : 57 . 8 vs 22 . 0 , P < 0 . 0001 ; IGF I / GHBP : 84 . 2 vs 48 . 8 , P=0 . 002 ; IGF I / IGFBP 3 : 36 . 8 vs 7 . 3 P=0 . 001 ; IGF I+IGF 2 : 77 . 8 vs 52 . 6 , P=0 . 01 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Like insulin like growth factor binding protein 3 ( IGFBP 3 ) , IGFBP 5 forms a ternary complex with insulin like growth factor ( IGF ) 1 or IGF 2 , and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No difference was found between mean IGFBP 4 serum levels in the two groups studied , while mean levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 5 , 25 ( OH ) D 3 , and markers of bone formation were significantly lower ( p < 0 . 006 ) in patients as compared with healthy subjects . ^^^ In pooled data from the normal and osteoporotic populations , age adjusted multiple regression models based on IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 5 were found to be highly predictive of serum OC ( R 2 = 19 % , p < 0 . 001 ) and BMD of femoral neck ( R 2 = 49 % , p < 0 . 0001 ) , consistent with an effect of the anabolic IGF components on overall bone formation rate . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
After 1 week off GH treatment , significantly lower levels of IGF 1 , IGF 2 , IGFBP 3 , and ALS were recorded ( P < 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In contrast , IGF 2 at an equimolar dose had little effect on proteolysis of recombinant human IGFBP 3 , whereas excess IGF 2 reproducibly inhibited recombinant human IGFBP 3 proteolysis by pregnancy serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We found severalfold increased serum levels of IGFBP 3 ( 2 fold ) , IGFBP 4 ( 5 fold ) and slightly increased IGF 2 levels in ESRF patients as well as a 2 . 6 fold increase in free IGF 1 in CRF patients with PTH < 100 pg / ml . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein ( IGFBP ) 3 bound IGF 1 and IGFBP 3 bound IGF 2 in growth hormone deficiency . ^^^ We have used an equation derived from the law of mass action to estimate serum IGFBP 3 bound IGF 1 and IGFBP 3 bound IGF 2 , as well as serum free IGF 1 and free IGF 2 , in 129 control children and adolescents ( 48 girls and 81 boys ) and in 13 patients with GHD . ^^^ Levels of serum total IGF 1 , total IGF 2 , IGFBP 1 , IGFBP 2 and IGFBP 3 were determined experimentally , while those of IGFBP 4 , IGFBP 5 and IGFPB 6 , as well as the 12 affinity constants of association of the two IGFs with the six IGFBPs , were taken from published values . ^^^ In controls , serum total IGF 1 , total IGF 2 , IGFBP 3 , IGFBP 3 bound IGF 1 , IGFBP 3 bound IGF 2 and free IGF 1 increased linearly with age , from less than 1 to 15 years , in the two sexes . ^^^ The mean SDS of serum total IGF 2 , IGFBP 3 bound IGF 2 and free IGF 2 were 1 . 25 + / 0 . 68 , 2 . 03 + / 0 . 87 and 0 . 59 + / 1 . 10 , respectively , in GHD . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
When autocrine IGF 2 was immuno neutralized or bound by the addition of rhIGF binding protein 3 ( IGFBP 3 ) beta casein mRNA expression was enhanced seven fold and three fold , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have extended the analysis of specimens to include measurements of GHBP , PGH , IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , and IGFBP 3 and have related these to maternal characteristics , fetal growth , and glycemia . ^^^ IGFBP 3 showed a significant correlation with IGF 1 , IGF 2 , and free and total PGH at K 28 and K 36 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) can inhibit cell growth by directly interacting with cells , as well as by forming complexes with IGF 1 and IGF 2 that prevent their growth promoting activity . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) can inhibit cell growth by directly interacting with cells , as well as by forming complexes with IGF 1 and IGF 2 that prevent their growth promoting activity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the SF , the following parameters were determined : Interleukin 1 beta , IL 6 , IL 8 , IL 1 RA , TNF alpha , Insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 as well as total proteinase activity and total collagenase activity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In adult animals , most circulating insulin like growth factor 1 ( IGF 1 ) and IGF 2 is sequestered in a 150 kDa complex composed of 1 molecule each of IGF , IGF binding protein 3 or 5 , and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured basal levels of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 and IGFBP 3 together with 24 h urinary GH excretion . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Studying deficient growth in this hypoplastic infant ( birth weight 1405 g , birth length 36 cm ) with dysmorphic features , the following changes in IGFs and IGFBPs were detected ( microg / l ) : IGF 1 : 26 . 5 vs 48 . 1 in healthy infants ; IGF 2 : 420 vs 728 ; IGFBP 2 : 931 vs 524 ; IGFBP 3 : 800 vs 1070 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
However , IGF 2 levels , IGFBP 4 profiles , and IGFBP 3 protease activity did not differ significantly between the two groups . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These samples were used to study binary complex formation with IGFBPs 1 to 6 using competitive IGF 2 binding assays and ternary complex formation with IGFBP 3 and IGFBP 5 . ^^^ As previously described , ternary complex formation by acid labile subunit ( ALS ) with IGFBP 3 and pro IGF 2 was greatly decreased compared with complex formation with mature IGF 2 . ^^^ These studies suggest that pro IGF 2 preferentially forms binary complexes with IGFBPs , and ternary complexes with IGFBP 5 , rather than ternary complexes with IGFBP 3 as seen predominantly in normal serum . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 mRNA is present in the chorionic mesoderm of placental villi from both these species and may modulate IGF 2 action through a paracrine mechanism . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Between d 2 and 14 of the estrous cycle , concentrations of progesterone , LH , IGF 2 , and binding activities of IGFBP 3 , IGFBP 2 , and the 27 to 29 kDa IGFBP in plasma did not differ ( P > . 10 ) among breeds . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Systemic levels contribute significantly to increased intraocular IGF 1 , IGF 2 and IGF BP 3 [ correction of IFG BP 3 ] in proliferative diabetic retinopathy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There was a positive correlation between IGFBP 4 and IGFBP 2 ( r=0 . 46 , P < 0 . 001 ) ; IGFBP 5 was positively correlated with IGF 1 ( r=0 . 59 , P < 0 . 001 ) , IGF 2 ( r=0 . 42 , P < 0 . 001 ) and IGFBP 3 ( r=0 . 47 , P < 0 . 001 ) and inversely correlated with IGFBP 1 ( r= 0 . 41 , P < 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Time course of fenretinide induced modulation of circulating insulin like growth factor ( IGF ) 1 , IGF 2 and IGFBP 3 in a bladder cancer chemoprevention trial . ^^^ We investigated the effect of fenretinide on circulating IGF 1 , IGF 2 and IGFBP 3 measured at yearly intervals during the 2 year treatment period and one year after treatment discontinuation in a predominantly male population of patients with superficial bladder cancer . ^^^ No significant effect was evident on IGFBP 3 , IGF 2 and the IGF I+IGF II / IGFBP 3 molar ratio , expressing the tissue availability of the mitogenic peptides , although IGF 2 and the molar ratio were lowered by treatment by an overall mean of 16 % and 15 % , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Large amounts of mRNA were detected for the type 1 receptor ( IGF 1R ) and for IGF binding protein ( IGFBP ) 5 , localised to the PT and PD , and less intense specific hybridisation signals were obtained with mRNAs for IGF 2 , type 2 receptor ( IGF 2R ) and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The aim of this study was to determine the effect of aging on serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) levels following myocardial infarction ( MI ) . ^^^ For four consecutive days , we monitored the variation of serum IGF 1 , IGF 2 and IGFBP 3 concentrations in 26 patients aged 19 71 years who were diagnosed with MI . ^^^ Serum IGF 1 , IGF 2 and IGFBP 3 were measured daily by double antibody radioimmunoassay . ^^^ MI patients in both groups showed significantly lower IGF 1 and IGF 2 ( IGFs ) with higher IGFBP 3 compared to age and sex adjusted levels of normal adults ( controls ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MATERIALS AND METHODS : We have investigated the effect of SA on prostate cancer cell ( DU 145 , LNCaP and PC 3 ) growth and expression of IGFs ( IGF 1 , IGF 2 and IGF 1 receptor ( IGF IR ) ) , IGFBP 3 and vascular endothelial growth factor ( VEGF ) . ^^^ Gene expression studies by RT PCR have demonstrated a decrease in mRNAs of IGF 2 in DU 145 , IGF 1 , and IGF IR in LNCaP , and IGF 2 and VEGF in PC 3 cells and an increase in IGFBP 3 in both DU 145 and PC 3 cells by treatment with SA . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Preoperatively , neutral chromatography demonstrated most of the immunoassayable IGFBP 3 in a high molecular weight form with a small amount of IGF 2 . ^^^ Postoperative samples showed a shift in IGF 2 which became increasingly associated with IGFBP 3 in both low and high molecular weight complexes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) were low on admission , and in the 8 patients whose serum IGF 1 levels failed to increase over 30 days , levels of the other proteins also remained low , while IGFBP 3 proteolytic activity increased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
From their recently determined amino acid sequences , these fragments correspond to the first 160 and 95 residues , respectively , of IGFBP 3 . 0 . 5 5 nM intact rhIGFBP 3 ( 1 264 ) , when pre incubated with 5 nM rhIGF 2 , dose dependently inhibited ( up to 100 % ) its mitogenic effect , via sequestration owing to its strong affinity for IGF 2 . ^^^ In a previous study , the biphasic effect of increasing dosages of recombinant human insulin like growth factor binding protein 3 ( rhIGFBP 3 ) on proliferation in the prostate carcinoma PC 3 cell line ( stimulation followed by depression ) was shown to reflect changes in the bioavailability of IGF 2 secreted by the cells , IGF 2 being the major factor responsible for their autocrine growth . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These contrasting backgrounds led us to investigate the relationship between serum IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and the presence of colorectal adenomas , known precursors of colorectal carcinoma , in 345 volunteers attending a screening flexible sigmoidoscopy trial ( entry criteria : healthy , aged 55 64 yr ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Expression levels of IGFBP 3 , an IGF 2 carrier protein , metalloproteinase 2 and cathepsin D were also increased to 200 , 340 , and 310 % of control levels , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In contrast to intact human IGFBP 3 and IGFBP 5 , this form of bovine IGFBP 3 forms ternary complexes preferentially with IGF 2 rather than IGF I . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study explored the relationships of serum insulin like growth factors , IGF 1 and IGF 2 , and their binding proteins ( IGFBP ) 2 and IGFBP 3 , with key clinicopathological parameters in 92 patients with colorectal cancer ( cases ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factors ( IGFs ) circulate in plasma as part of a 150 kD complex that also contains IGF binding protein 3 ( IGFBP 3 ) , a protein that binds IGF 1 and IGF 2 with high affinity , and an acid labile subunit ( ALS ) that does not directly bind IGFs . ^^^ Significant correlations ( p < 0 . 001 ) were seen between total and free ALS concentrations and IGF 1 ( r = 0 . 50 and 0 . 60 , respectively ) , free IGF 1 ( r = 0 . 37 and 0 . 36 ) , IGF 2 ( r = 0 . 37 and 0 . 27 ) , IGFBP 1 ( r = 0 . 48 and 0 . 49 ) , IGFBP 2 ( r = 0 . 44 and 0 . 51 ) and IGFBP 3 ( r = 0 . 67 and 0 . 59 ) at all Tanner stages . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 3 , and total and free ALS levels were significantly decreased at diagnosis . ^^^ Normalization of IGF 2 and IGFBP 3 occurred 6 months after diagnosis , and normalization of IGF 1 and total and free ALS occurred 1 yr after terminating therapy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
GH treatment normalized serum IGF 1 in a majority of the patients and increased IGFBP 3 and 5 as well as IGFBP 4 and IGF 2 to values within normal range . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Furthermore , we assessed serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) levels and IGFBP 3 circulating forms . ^^^ Overall , IDDM children showed reduced levels of IGF 1 ( mean + / SD , 0 . 65+ / 1 . 9 z score ) and normal levels of IGF 2 ( mean + / SD , 0 . 05+ / 1 . 2 z score ) and IGFBP 3 ( mean + / SD , 0 . 06+ / 1 . 2 z score ) . ^^^ However , whereas patients younger than 6 yr showed normal values of IGF 1 , IGF 2 , and IGFBP 3 , these peptides were significantly reduced in older subjects compared with either younger IDDM children or controls ( P < 0 . 01 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 , and prostate specific antigen as predictors of clinical prostate cancer . ^^^ In a prospective , cohort , case control study in the Baltimore Longitudinal Study on Aging population , we examined prostate volume by magnetic resonance imaging , and prostate specific antigen ( PSA ) , IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in sera obtained approximately 9 yr before diagnosis of prostate cancer in cases ( n = 72 ) or age matched controls ( n = 127 ) and in 76 additional Baltimore Longitudinal Study on Aging men ( normal subjects ) with measured prostate volumes and no prostate cancer . ^^^ Adjusted ORs for the high vs . low tertile were : for IGF 1 , 3 . 1 [ confidence interval ( CI ) , 1 . 1 8 . 7 ] ; for IGF 2 , 0 . 2 ( CI , 0 . 07 0 . 6 ) ; for IGFBP 3 , 0 . 71 ( CI , 0 . 3 1 . 7 ) ; and for PSA , 12 . 5 ( CI , 3 . 8 40 . 9 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Size at birth and cord blood levels of insulin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 3 , and the soluble IGF II / mannose 6 phosphate receptor in term human infants . ^^^ We measured cord blood levels of IGF 2 , soluble IGF2R , insulin , IGF 1 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 and examined their relationships to weight , length , head circumference , ponderal index , and placental weight at birth in 199 normal term singletons . ^^^ IGF 2 levels correlated with levels of IGF 1 ( r = 0 . 29 ; P < 0 . 0005 ) , IGFBP 3 ( r = 0 . 45 ; P < 0 . 0005 ) , and soluble IGF2R ( r = 0 . 20 ; P < 0 . 005 ) . ^^^ Correlations between the IGFs and size at birth were stronger in nonprimiparous pregnancies ; in these , IGF 1 ( r = 0 . 52 ; P < 0 . 0005 ) , IGFBP 3 ( r = 0 . 41 ; P < 0 . 0005 ) , and the IGF 2 to IGF2R ratio ( r = 0 . 40 ; P < 0 . 0005 ) were most closely related to placental weight , together accounting for 39 % of its variance . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Ovarian follicular concentrations of activin , follistatin , inhibin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor in spontaneous menstrual cycles of normal women of advanced reproductive age . ^^^ FF was analyzed for estradiol , progesterone , testosterone , androstenedione , inhibin A and B , total activin A , total follistatin , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , IGFBP 3 , and vascular endothelial growth factor ( VEGF ) concentrations . ^^^ We found that the dominant follicles from older women contain normal concentrations of steroids , inhibin A and B , IGF 2 , IGFBP 2 , and IGFBP 3 ; increased concentrations of follistatin , activin A , and VEGF ; and decreased concentrations of IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The elevated IGF 2 level ( 787 ng / ml ; 300 500 ) and decreased IGF binding protein 3 ( 1 . 6 mg / l ; 2 5 ) indicated a high free IGF 2 activity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Further , one finds reduced synthesis of IGFBP 3 , which inhibits the action of IGF 2 by inducing a complex with the paraneoplastic protein . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Body composition ( dual energy 10 ray absorptiometry ) , resting VO 2 ( indirect calorimetry ) , resting HR ( telemetry ) and serum IGF 1 , IGF 2 , IGFBP 3 and acid labile subunit ( ALS ) were measured at baseline , after 12 weeks of GH administration and , additionally in the GH group , 1 , 2 , 3 , 4 , 5 and 9 days after GH discontinuation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN : DNA synthesis and apoptosis in Saos 2 / B 10 cells were measured in response to IGF 1 , IGF 2 , IGFBP 3 and TGF beta 2 , and to type 1 IGF receptor ligands with poor affinity for IGFBP 3 ( [ QAYL ] IGF 1 and insulin ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) 1 , IGF 2 , free IGF 1 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 and IGFBP 3 were measured by radioimmuno / immunoradiometric assays . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , IGFBP 3 by immunoassays , IGFBP 3 by Western ligand blotting ( WLB ) and finally in vitro IGFBP 3 proteolysis by a ( 125 ) 1 IGFBP 3 degradation assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Classical and atypical GHIS groups were compared in terms of ( 1 ) phenotypic features , including high pitched voice , sparse hair , blue sclera , hypoglycaemia , microphallus , ( 2 ) birth length , height SDS , and ( 3 ) basal IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , GHBP and increase in IGF 1 on IGF 1 generation testing . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The present study was performed to analyze the correlations of circulating levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 with biochemical markers of bone metabolism and parameters of the urea kinetic model which reflect nutritional conditions in hemodialysis patients . ^^^ We measured the serum levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 . ^^^ However , the serum levels of IGF 2 , IGFBP 1 , and IGFBP 3 in the hemodialysis patients were significantly higher than those in the control group . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The ternary complex , composed of IGF 1 or IGF 2 , IGF binding protein 3 , and the acid labile subunit , is responsible for transport of the majority of the IGF 1 and IGF 2 present in the circulation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin , IGF 1 and IGF 2 brought about a 1 . 5 2 fold enhancement of IGFBP 3 release at 7 . 5 30 nM concentrations . ^^^ We conclude that , in addition to GH , also insulin , IGF 1 and IGF 2 and glucocorticoids can modulate IGFBP 3 secretion by human hepatoma cells . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Levels of IGF binding protein 3 ( IGFBP 3 ) , which may induce apoptosis by IGF dependent or independent mechanisms , were increased by butyrate and trichostatin A ; IGF 2 augmented this effect . ^^^ In contrast , IGF 2 promotes histone deacetylase dependent IGFBP 3 expression and histone deacetylase independent migration . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , and IGFBP 3 in white and African American men at increased risk of prostate cancer . ^^^ OBJECTIVES : To further investigate the relationship between the plasma levels of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , growth hormone , testosterone , and demographic factors , particularly race , within a group of men at increased risk of prostate cancer development . ^^^ METHODS : Enzyme linked immunosorbent assays or an immunosorbent assay was used to quantitate the plasma levels of IGF 1 , IGF 2 , IGFBP 3 , growth hormone , and testosterone . ^^^ Overall , the IGF 1 plasma levels correlated positively with the IGF 2 , IGFBP 3 , and growth hormone levels and the IGF 2 plasma levels correlated negatively with the testosterone levels . ^^^ CONCLUSIONS : Our results demonstrate that lower plasma levels of IGFBP 3 and IGF 2 are associated with race in a population of men at increased risk of developing prostate cancer . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RIA and RT PCR demonstrated that the genital skin fibroblast strains expressed IGF 2 , IGF binding protein 2 , and IGF binding protein 3 , but no IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : Serum IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were measured in healthy adults and acromegalics . ^^^ RESULTS : For acromegalic patients , GH was significantly correlated with IGF 1 ( r = 0 . 50 ; P < 0 . 001 ) and IGFBP 3 ( r = 0 . 29 ; P = 0 . 03 ) but not IGF 2 or IGFBP 2 . ^^^ The ( IGF 1 + IGF 2 ) / IGFBP 3 molar ratios were remarkably constant in both healthy adults and acromegalics , but the relationships of the ligands individually with IGFBP 3 were not linear : as IGFBP 3 increased , IGF 1 also increased whereas IGF 2 initially increased but then decreased . ^^^ IGFBP 2 did not correlate with IGF 2 , but molar concentration significantly correlated with the IGF II / IGFBP 3 molar ratio ( r = 0 . 40 ; P = 0 . 001 ) . ^^^ The ( IGF 1 + IGF 2 ) / IGFBP 3 molar ratio appears to be pivotal in determining IGF 2 values , which , in turn , expressed as a ratio of IGFBP 3 , is related to IGFBP 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 levels vary with age after puberty , and this is complicated by the maintenance of IGF binding protein 3 levels by IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) binds IGF 1 and IGF 2 with high affinity , at least an order of magnitude higher than the affiniy of the IGFs for the IGFIR . ^^^ IGFBP 3 inhibited IGF 1 , IGF 2 , Des ( 1 3 ) IGF 1 and Long ( R 3 ) IGF 1 induced IGFIR phosphorylation in a dose dependent manner at similar concentration range but not QAYL induced IGFIR P . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) binds IGF 1 and IGF 2 with high affinity , at least an order of magnitude higher than the affiniy of the IGFs for the IGFIR . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 and IGFBP 3 in prediagnostic serum : association with colorectal cancer in a cohort of Chinese men in Shanghai . ^^^ This is the first study to investigate the associations of IGF 1 , IGF 2 and IGFBP 3 concentrations with the risk of colorectal cancer in prospectively collected blood samples from an Oriental population . ^^^ IGF 1 , IGF 2 and IGFBP 3 were measured in the serum of 135 men who developed colorectal cancer over 12 years of follow up and 661 control subjects drawn from the cohort , who were matched to the index cases by neighbourhood of residence , age , and year and month of sample collection . ^^^ IGF 2 and IGFBP 3 , on the other hand , exhibited statistically significant , positive associations with colorectal cancer risk when cases were confined to those diagnosed within a relatively short time period after enrollment ( within 8 years ) . ^^^ After adjustment for body mass index , cigarette smoking and alcohol intake , men in the highest versus the lowest quintile of IGF 2 and IGFBP 3 showed odds ratios of 2 . 74 ( 95 % Cl = 1 . 67 4 . 50 ; 2 sided P for trend = 0 . 0008 ) and 2 . 85 ( 95 % Cl = 1 . 69 4 . 81 ; 2 sided P for trend = 0 . 01 ) , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No significant differences in IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 , IGFBP 3 , soluble IGF 2 receptor levels ( IGF2R ) , IGF II / IGF2R ratio , and relative amounts of IGFBP 3 circulating forms were found between CG and NCG children . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To provide further insight into the effect of tamoxifen at low doses on the IGF system , we have correlated the drug serum levels attained after 2 months of either placebo ( n = 32 ) , tamoxifen 20 mg / day ( n = 26 ) , 10 mg / day ( n = 23 ) or 10 mg / every other day ( n = 29 ) with the changes in IGF 1 , Insulin like Growth Factor 2 ( IGF 2 ) , Insulin like Growth Factor Binding Protein 1 ( IGFBP 1 ) , Insulin like Growth Factor Binding Protein 3 ( IGFBP 3 ) , and IGF I / IGFBP 3 ratio . ^^^ Compared with placebo , tamoxifen induced a mean + / standard error ( SE ) reduction of IGF 1 of 16 . 9 + / 7 . 8 % , p < 0 . 05 , a non significant increase of 22 . 9 + / 12 . 2 % in IGF 2 , an increase in IGFBP 1 of 49 . 3 + / 22 . 7 % , p < 0 . 05 , and a non significant change of IGFBP 3 ( 4 . 0 % + / 9 . 2 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) can stimulate apoptosis and inhibit cell proliferation directly and independently of binding IGFs or indirectly by forming complexes with IGF 1 and IGF 2 that prevent them from activating the IGF 1 receptor to stimulate cell survival and proliferation . ^^^ To assess the general importance of IGF independent mechanisms , we have generated human IGFBP 3 mutants that can not bind IGF 1 or IGF 2 by substituting alanine for six residues in the proposed IGF binding site , Ile ( 56 ) / Tyr ( 57 ) / Arg ( 75 ) / Leu ( 77 ) / Leu ( 80 ) / Leu ( 81 ) , and expressing the 6m hIGFBP 3 mutant construct in Chinese hamster ovary cells . ^^^ PC 3 cells are known to synthesize and respond to IGF 2 , so that IGFBP 3 could potentially act by either IGF dependent or IGF independent mechanisms . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Fifteen IUGR and 12 normal short prepubertal subjects had a 90 min frequently sampled 4 glucose tolerance test performed to measure plasma glucose , insulin , IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 1 . ^^^ In conclusion , short IUGR children have higher plasma IGF 1 , IGF 2 , and IGFBP 3 , when compared with normal children matched for height , weight , and pubertal status . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
AIMS / HYPOTHESIS : The aim of this study was to investigate the role of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) and IGFBP 3 gene variants on the metabolic changes observed in response to a 100 day overfeeding protocol conducted with 12 pairs of monozygotic twins . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Malignant lymphoblasts of T cell origin more often expressed IGFBP 2 and IGFBP 5 , whereas IGF 2 and IGFBP 3 expression was seen more often in lymphoblasts of B cell origin . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No differences were observed in the expression of IGF 2 , IGFBP 3 and 6 and IGF IR throughout the lifespan of the corpus luteum . ^^^ However , functional studies showed that nitric oxide donors elicited a stimulatory action on production of IGF 1 in corpora lutea from the early luteal phase ( 80 % ) and on production of IGFBP 1 in corpora lutea from the late luteal phase ( 50 % ) ( P < 0 . 05 ) , whereas production of IGF 2 and IGFBP 3 was not affected by nitric oxide . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
BMD in total body , distal and UD radius , and femoral neck was positively correlated with serum IGF 1 ( r = 0 . 31 to 0 . 49 ) , IGF 2 ( r = 0 . 32 to 0 . 48 ) , IGFBP 3 ( r = 0 . 37 to 0 . 53 ) , and free androgen index ( FAI ) ( r = 0 . 32 to 0 . 40 ) , and negatively with IGFBP 1 ( r = 0 . 37 to 0 . 41 ) and IGFBP 2 ( r = 0 . 29 to 0 . 41 ) levels . ^^^ Age correlated negatively with serum IGF 1 , IGF 2 , IGFBP 3 , FAI , estradiol / SHBG ratio , and BMD in total body , distal and UD radius , and femoral neck , and positively with IGFBP 1 , IGFBP 2 , and SHBG levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the current study , growth inhibition by IGF 1 and IGF 2 was blocked by concurrent addition of IGFBP 3 , implying that colon cancers with elevated IGFBP 3 levels would be selected for in vivo because they could bind and inactivate high serum IGF 1 levels and continue to proliferate . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Blood samples were collected for insulin , C peptide , glucose , HbA1c , insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Hypertrophy induced by IGF 1 was blocked by IGF binding protein 3 , which binds IGF 1 , while that induced by IGF 2 was blocked by antibodies against IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To explore the effect of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) on the fetal growth . ^^^ Serum IGF 1 , IGF 2 and IGFBP 3 were measured by radioimmunoassay ( RIA ) and immunoradiometric analysis ( IRMA ) . ^^^ The fetal IGF 1 , IGF 2 and IGFBP 3 levels in FGR group were significantly lower than those in control group ( P < 0 . 01 ) . ( 5 ) The levels of fetal IGF 1 , IGF 2 , IGFBP 3 were positively correlated with birth weight ( r = 0 . 61 , r = 0 . 51 and 0 . 63 , P < 0 . 01 ) and placental weight ( r = 0 . 47 , r = 0 . 56 and 0 . 48 , P < 0 . 01 ) . ^^^ The levels of fetal IGF 1 , IGF 2 and IGFBP 3 were no different between macrosomia group and control group . ^^^ CONCLUSIONS : This study suggest that ( 1 ) IGF 1 , IGF 2 and IGFBP 3 can not pass through the placenta . ( 2 ) The betal IGF 1 , IGF 2 and IGFBP 3 may have the close relationship with the fetal growth . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured the binding of IGF 1 and IGF 2 to recombinant human N terminal [ residues 1 97 ; recombinant human IGF binding protein 3 ( 1 97 ) ( rhIGFBP 3 ( 1 97 ) ) ] and C terminal ( residues 98 264 ; rhIGFBP 3 ( 98 264 ) ) IGFBP 3 fragments and compared it with IGF binding to intact IGFBP 3 using biosensor analysis . ^^^ These experiments showed that IGF 1 and IGF 2 bind to IGFBP 3 with affinities of 4 5 10 10 ( 9 ) M ( 1 ) and similar binding kinetics . ^^^ Binding of insulin to IGFBP 3 and its N and C terminal fragments and of IGF 1 and IGF 2 to the structurally related proteins mac 25 and connective tissue growth factor was also investigated . ^^^ Weak insulin binding to full length IGFBP 3 could be demonstrated in a few experiments , but we found that binding of IGF 1 , IGF 2 , and insulin to mac 25 or connective tissue growth factor was below the detection limit of the biosensor instrument . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Treatment of HepG 2 cells with either human recombinant IGFBP 3 ( hrIGFBP 3 ) or IGF 2 antibody led to a significant reduction in cell proliferation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) was low and electrophoretic profile of IGF 2 was the ' big IGF 2 ' type . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) was low and electrophoretic profile of IGF 2 was the ' big IGF 2 ' type . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : Birth weight ( BW ) , birth length ( BL ) , ponderal index , head circumference ( HC ) , and cord serum levels of albumin , prealbumin , retinol binding protein ( RBP ) , total and free IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and leptin were significantly lower , whereas levels of IGFBP 1 , IGFBP 2 , and IL 6 were significantly higher in preterm than in term neonates ( P < 0 . 05 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : Plasma levels of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 were determined from the fasting bloods of 206 BPH cases admitted for treatment and 306 randomly selected population controls in Shanghai , China . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Plasma levels of IGF 1 , IGF 2 , and IGFBP 3 were measured using commercial ELISA kits ( Diagnostic Systems Laboratories , Webster , TX ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured plasma IGF 1 , IGF 2 , and IGF BP 3 in a cross section of 210 men and 171 postmenopausal women enrolled in research as healthy controls . ^^^ Overall , IGF 1 and IGF BP 3 levels did not differ by gender , although men had significantly higher molar ratios of IGF 1 to IGF BP 3 and lower plasma IGF 2 than women without recent HRT use . ^^^ Results for ratios of IGF 1 and IGF 2 to IGF BP 3 generally reflected those for IGF 1 and IGF 2 levels , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN AND MEASUREMENTS : Serum levels of IGF 1 , IGF 2 and IGFBP 3 , and peripheral markers leptin , C terminal propeptide of type 1 collagen ( PICP ) and N terminal propeptide of type 3 collagen ( PIIINP ) , alkaline phosphatase ( AP ) and osteocalcin ( OC ) were measured at the start and after 2 and 12 weeks of periods of no treatment , GH 1 . 5 IU / m2 and GH 3 . 0 IU / m2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have investigated the mitogenic properties of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and epidermal growth factor ( EGF ) on human breast epithelial cells ( HBEC ) in primary culture . ^^^ IGFBP 3 inhibits the stimulation by IGF 1 , IGF 2 and IGF 1 plus EGE In addition , it appears that IGFBP 3 has an inhibitory effect on HBEC growth that is IGF independent . ^^^ This study is the first to address the effects of IGF 1 , IGF 2 and IGFBP 3 alone and in combination with EGF on HBEC growth in primary culture . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In this prospective , observational study IGF 1 , IGF 2 , IGF binding protein 2 , IGF binding protein 3 , and acid labile subunit were measured by immunoassays in the wound fluid and plasma of young ( 23 . 5 + / 3 . 3 years ) and elderly ( 78 . 9 + / 6 . 2 years ) patients before and daily for 4 days after elective surgery . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We measured serum IGF 1 , IGF 2 , and IGF binding protein 3 ( IGFBP 3 ) in 194 case subjects who subsequently died from lung cancer during an 8 year follow up and in 9351 controls . ^^^ The odds ratios ( ORs ) , adjusted for smoking and other covariates , were smaller with higher levels of IGF 2 and IGFBP 3 . ^^^ The ORs across quartiles were 0 . 41 ( 95 % confidence interval [ CI ] , 0 . 27 0 . 63 ) , 0 . 47 ( 0 . 31 0 . 71 ) , and 0 . 67 ( 0 . 46 0 . 98 ) for IGF 2 ( trend P=0 . 018 ) , and 0 . 55 ( 95 % CI , 0 . 37 0 . 81 ) , 0 . 54 ( 0 . 36 0 . 82 ) , and 0 . 67 ( 0 . 45 1 . 01 ) for IGFBP 3 ( trend P=0 . 037 ) . ^^^ Limiting subjects to those followed for 3 years strengthened the negative associations of IGF 2 and IGFBP 3 , whereas the ORs for IGF 1 generally decreased . ^^^ A higher level of circulating IGFBP 3 and / or IGF 2 may decrease lung cancer risk . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
As reported for native IGFBPs , IGF 2 bound with affinity higher than IGF 1 to recombinant IGFBP 3 and IGFBP 4 ( K ( D ) of < 0 . 05 nmol ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Northern blots revealed mRNAs for IGF 2 , IGF binding protein 2 ( IGFBP 2 ) , and IGFBP 3 in all oviductal and endometrial tissues . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : Cross sectional study : urinary leptin , measured in the first morning urine voided and expressed as ng excreted overnight , and serum concentrations of leptin , IGF 1 , IGF 2 , IGFBP 3 and IGFBP 1 were determined in a cohort of 188 healthy schoolchildren aged 5 19 years ( 88 males , 100 females ) . ^^^ In the cross sectional study urinary leptin correlated significantly with serum IGF 1 ( r = +0 . 41 , P = 0 . 001 ) , IGF 2 ( r = +0 . 19 , P = 0 . 05 ) , IGFBP 3 ( r = +0 . 29 , P = 0 . 001 ) and IGFBP 1 ( r = 0 . 25 , P = 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum T and F IGF 1 and IGF 2 were determined using noncompetitive time resolved monoclonal immunofluorometric assays , IGFBP 1 was determined by an in house RIA , IGFBP 2 by a novel in house time resolved immunofluorometric assay , whereas IGFBP 3 by an immunoradiometric assay . ^^^ Specifically , mean T IGF 1 for patients group was 131 ( 61 ) , F IGF 1 was 0 . 47 ( 0 . 16 ) , T IGF 2 was 1056 ( 300 ) , F IGF 2 was 1 . 45 ( 0 . 54 ) , IGFBP 1 was 33 ( 14 ) , IGFBP 2 was 848 ( 377 ) , and finally IGFBP 3 was 3953 ( 1422 ) . ^^^ For controls , mean T IGF 1 was 146 ( 51 ) , F IGF 1 was 0 . 85 ( 0 . 43 ) , T IGF 2 was 939 ( 197 ) , F IGF 2 was 1 . 53 ( 0 . 53 ) , IGFBP 1 was 44 ( 24 ) , IGFBP 2 was 764 ( 316 ) and finally IGFBP 3 was 3721 ( 1017 ) . ^^^ Furthermore , no statistically differences emerged in the ratio between molar weights of T IGF I / IGFBP 3 and T IGF II / IGFBP 3 , as well as to the F / T IGF 1 and F / T IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No training induced differences in IGF 1 , IGF 2 , IGF l : IGF 2 ( IGF 1 : IGF 2 ) , and IGFBP 3 were detected . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : The expression of IGF 2 mRNA , IGF 1 R mRNA and IGFBP 3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The results were analyzed through multiple logistic regression and controlled for known risk factors for endometrial cancer , leptin , as well as major components of the IGF system ( IGF 1 , IGF 2 , and IGF binding protein 3 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The expression of AR , IGF 2 and IGFBP 2 , IGFBP 3 , IGFBP 5 , and 5alpha reductase type 2 did not differ among the different types of tumors . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To this end , we evaluated the behavior not only of IGF 1 but also of IGF 2 , IGF binding protein ( IGFBP ) 3 , and acid labile subunit , the subunits that constitute the main IGF complex in circulation ( 150 kDa complex ) , as well as of IGFBP 1 and IGFBP 2 , which are negatively regulated by GH and , like IGFBP 3 , able to influence the bioavailability of the IGFs . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
With in situ hybridization , insulin like growth factor 2 ( IGF 2 ) mRNA and insulin like growth factor binding protein 2 ( IGFBP 2 ) , 3 and 6 were all detected in the placentome capsule ; IGF 2 mRNA was also found in the mesoderm of the fetal villi and IGFBP 3 and IGFBP 6 were present in the caruncular stroma of the maternal villi . ^^^ Ewes of moderate condition , which had the smallest placentae , had the greatest placental expression of IGF 2 , IGFBP 2 and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Between 14 and 19 days of age , sharp falls in the quantities of IGF 1 , IGF 2 , IGFR 1 , IGFR 2 , IGFBP 3 , IGFBP 5 , and IGFBP 6 mRNAs were observed , whereas the quantity of IGFBP 4 mRNA rose sharply during the same period . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have undertaken a case control study among Greek women to explore the relation of major components of the IGF system ( IGF 1 , IGF 2 and IGF binding protein 3 , IGFBP 3 ) with endometrial cancer risk . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Individuals with serum IGF 2 levels in the upper quartile of the normal range ( and IGF binding protein 3 levels in the lower quartiles ) have a relative risk for developing breast , prostate , colon and lung cancer . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
With respect to the gene expression of other IGF system components , VA deficiency caused a twofold induction of IGF 1 receptor ( IGF IR ) mRNA in the heart and a twofold reduction in IGFBP 6 mRNA in the lungs , but did not alter the expression of IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 4 or IGFBP 5 in all tissues examined . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Equimolar NBP 3 + CBP 3 bound IGF 2 with an affinity ( 12 . 2 liter / nmol ) only 4 fold lower than that of the IGFBP 3 IGF 2 complex and IGF 1 with an affinity ( 3 . 2 liter / nmol ) 13 fold lower than IGFBP 3 IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In random normal human adult sera ( n = 37 ) , the median IGFBP rP 1 was 21 . 0 micro g / liter , and values did not correlate with levels of IGF 1 ( r = 0 . 085 , P = 0 . 61 ) , IGF 2 ( r = 0 . 051 , P = 0 . 75 ) , or IGFBP 3 ( r = 0 . 061 , P = 0 . 74 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied the plasma concentrations of IGF 1 , IGF 2 , IGF binding protein 3 ( BP 3 ) , and GH in cord blood taken from the offspring of 1650 singleton Caucasian pregnancies born at term and related these values to birth weight , length , and head circumference . ^^^ Cord plasma concentrations of IGF 1 , IGF 2 , and IGFBP 3 were influenced by factors influencing birth size : gestational age at delivery , mode of delivery , maternal height , and parity of the mother . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
End points included changes in fasting insulin , glucose , insulin resistance , IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , and IGF 1 : IGFBP 3 molar ratio between baseline and week 15 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Concentrations of IGF 1 , IGF 2 and IGFBP 3 significantly decreased , and those of GHBP slightly increased with age from 20 to 92 years ( n=327 r= 0 . 64 p < 0 . 0001 ; n=45 r= 0 . 44 p < 0 . 003 ; n=91 r= 0 . 23 p < 0 . 03 and n=61 r=0 . 26 ; p < 0 . 05 respectively ) . ^^^ Our results suggest that the age related decline in plasma levels of IGF 1 , IGF 2 and IGFBP 3 occurs independently from malnutrition and inflammation processes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Within the framework of a large ( 769 patients ) , randomised double blind clinical trial comparing exemestane ( EXE ) with megestrol acetate ( MA ) , serum 17 beta estradiol ( E 2 ) , estrone ( E 1 ) , estrone sulphate ( E1S ) , bone alkaline phosphatase ( BAP ) , carboxy terminal cross linking telopeptide of type 1 collagen ( ICTP ) and the components of insulin like growth factor ( IGF ) family ( IGF 1 , IGF 2 and IGFBP 3 ) were determined in 53 patients ( 24 randomised to EXE and 29 ramdomised to MA ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The significantly reduced levels of IGF 1 ( p < 0 . 001 ) , IGF 2 ( p=0 . 017 ) and IGFBP 3 and the increased presence of IGFBP 2 were found in patients with echinococcosis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 , IGF 2 , and IGF binding protein 3 were inversely associated with the presence of neonatal jaundice and blood creatinine , after controlling for blood protein levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Human fibroblasts , glioblastoma cells , and murine myoblasts were used to determine the kinetics of IGF 2 binding to cell surface IGFBP 3 , IGFBP 5 , and the IGF 1R , respectively . ^^^ Zn2+ , Cd2+ , and Au3+ , but not La3+ , decreased total binding and the affinity for [ 125I ] IGF 2 association with IGFBP 3 and IGFBP 5 . ^^^ Together with the current work , these findings imply that Zn2+ acts in vivo to prevent secreted IGF 2 from binding to IGFBP 3 and IGFBP 5 , thus maintaining IGF 2 in an `` active state , ' ' i . e . , readily available for IGF 1R association . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Our aim was to evaluate the role of IGFBP 2 levels together with insulin like growth factor ( IGF ) 1 , IGF 2 and IGFBP 3 in the diagnostic work up of patients with hepatocellular carcinoma ( HCC ) . ^^^ METHODS : In 50 ( 39 males , 11 females ) histologically confirmed and TNM graded patients with HCC who had not received adjuvant chemotherapy , the basal serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and alpha fetoprotein ( AFP ) were measured . ^^^ RESULTS : The levels of IGF 1 , IGF 2 and IGFBP 3 were diminished , as is the case when nutrition , hepatic function and growth hormone ( GH ) secretion are decreased . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In any tumors that arose in animals injected with the IGFBP 3 expressing cells , there was a complete loss of IGFBP 3 activity , as measured by binding to IGF 1 and IGF 2 proteins . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : Our hypothesis is that insulin like growth factor binding protein 3 ( IGF BP 3 ) would downregulate epidermal growth factor receptor ( EGF R ) levels in cervical cancer cell lines , thereby reducing cellular IGF 2 and angiogenesis related vascular endothelial cell growth factor ( VEGF ) . ^^^ METHODS : We determined the cellular levels of EGF R , IGF 2 , and VEGF in the cervical cancer cell lines HeLa , ME 180 ( both positive for human papilloma virus ; HPV ) , and HT 3 ( HPV negative ) , following their treatment with IGF BP 3 . ^^^ RESULTS : Treatment with IGF BP 3 significantly reduced the levels ( mean intensity per pixel ) of EGF R , IGF 2 , and VEGF in all three cell lines and IGF 1 receptor ( IGF IR ) in representative ME 180 cell line . ^^^ CONCLUSIONS : Levels of EGF R , IGF 2 , IGF IR , and VEGF are significantly reduced following treatment with IGF BP 3 in cervical cancer . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We have prospectively investigated maternal serum levels of IGF 1 , IGF 2 , and the main binding protein insulin like growth factor binding protein 3 ( IGFBP 3 ) in women with and without preeclampsia . ^^^ CONCLUSION : In women who developed preeclampsia , we found no support for the hypothesis that the disease was preceded by lower serum levels of IGF 1 and IGF 2 , or higher serum levels of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Ghrelin was also negatively correlated to IGF 1 ( rs = 0 . 48 , P < 0 . 001 ) , IGFBP 3 ( rs = 0 . 32 , P < 0 . 001 ) and leptin ( rs = 0 . 22 , P = 0 . 02 ) but not IGF 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Determination of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 levels in serum and plasma : comparisons using the Bland Altman method . ^^^ Blood was obtained from 47 healthy volunteers ( age 21 72 years ) in serum , heparin plasma and EDTA plasma , and IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 measured , and results compared . ^^^ Mean values for IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 determined in all three media were generally comparable ; correlations were generally strong and significant ( P < 0 . 001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Evidence is presented that bovine somatotrophin ( bST ) treatment of lactating dairy cows enhances both expression of oviductal insulin like growth factor 2 ( IGF 2 ) mRNA and endometrial insulin like growth factor binding protein 3 ( IGFBP 3 ) mRNA between day 3 and day 7 of the oestrous cycle . mRNA encoding growth hormone ( GH ) receptor in endometrial tissues increased between day 3 and day 7 of the oestrous cycle . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : We aimed to further document that elevated levels of circulating insulin like growth factor 2 ( IGF 2 ) are associated with cervical cancer and to test the hypothesis that there may be an inverse association between IGF 2 and IGF binding protein 3 ( IGF BP 3 ) . ^^^ METHODS : Serum IGF 2 and IGF BP 3 levels were measured , using ELISA kits ( Diagnostic Systems Laboratories ) , in 23 controls ; 16 ASC US with normal biopsies ; 14 ASC US with advanced CIN ; 2 pretherapy CIN 1 ; 8 successfully treated CIN 1 ; 24 persistent CIN 1 ; 14 pretherapy CIN II / III ; 10 posttherapy CIN II / III with normal biopsies ; 18 persistent CIN II / III ; 7 with pretherapy cervical cancer ; 19 with posttherapy cervical cancer under remission ; 15 with posttherapy persistent / recurrent cervical cancer ; 10 with persistent ovarian or endometrial cancer ; and 3 with endometrial or vulvar with cervical cancer . ^^^ CONCLUSION : Serum IGF 2 may be a reliable marker for early diagnosis and monitoring therapy efficacy ( sensitivity and specificity of 100 % versus normal controls ) , while IGF BP 3 levels can be reliably used to predict prognosis . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We further report that serum IGF 1 , IGF 2 and IGFBP 3 all show positive correlations with myoblast retrieval in control patients , but that with the exception of IGFBP 3 these correlations are lost in malignancy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Assays of IGF 1 , IGF 2 , IGFBP 2 and IGFBP 3 were performed on cases and 2 controls matched on age , recruitment center and calendar time . ^^^ Controlling for smoking history and IGFBP 3 strengthened associations with cancer for both IGF 1 ( OR = 3 . 00 ; 95 % CI = 1 . 50 6 . 01 ; p ( trend ) 0 . 005 ) and IGF 2 ( OR = 2 . 02 ; 95 % CI = 1 . 07 3 . 84 ; p ( trend ) = 0 . 04 ) Associations between the IGFs and cancer risk were stronger for advanced cases . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We examined associations of stature and body mass index with IGF 1 , IGF 2 , and IGF binding protein ( IGFBP ) 2 and IGFBP 3 in 274 men aged 50 70 yr to investigate whether variations in growth factor levels underlie associations of anthropometry with a number of adult diseases . ^^^ Height and leg and trunk length were not strongly associated with circulating levels of IGF 1 , IGF 2 , or IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In colostrum fed calves , DEXA decreased ( P < 0 . 05 ) mRNA levels of IGF 1 ( esophagus , fundus , duodenum , and ileum ) , IGF 2 ( fundus ) , IGFBP 2 ( fundus ) , IGFBP 3 ( fundus ) , IGF1R ( esophagus , ileum , and colon ) , IGF2R ( fundus ) , GHR ( fundus ) , and InsR ( esophagus , fundus ) , but in formula fed calves DEXA increased mRNA levels of IGF 1 ( esophagus , rumen , jejunum , and colon ) . ^^^ Furthermore , DEXA increased ( P < 0 . 05 ) mRNA levels of IGF 2 ( pylorus ) , IGFBP 3 ( duodenum ) , IGF2R ( pylorus ) , and GHR ( ileum ) , but decreased mRNA levels of IGFBP 2 ( ileum ) , and IGF1R ( fundus ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
BACKGROUND : The purpose of the current study was to investigate the association between insulin resistance ( which was measured using fasting blood C peptide ) and its joint association with insulin like growth factors ( IGF 1 , IGF 2 , and IGF binding protein 3 [ IGFBP 3 ] ) on the risk of breast carcinoma . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : To examine whether measurement of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 or IGFBP 3 , alone or in combination , enhanced the specificity of prostate cancer detection among men with a prostate specific antigen ( PSA ) level of 3 ng / mL or greater beyond that achieved by the free / total PSA index . ^^^ Assays of IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , and free and total PSA were performed . ^^^ When all biopsies were included , the performance of the free / total PSA index ( AUC 0 . 73 ) was significantly greater than for IGF 1 ( AUC 0 . 59 ; P < 0 . 0001 ) , IGF I / PSA ratio ( AUC 0 . 65 ; P = 0 . 002 ) , IGF 1 + IGFBP 3 ( AUC 0 . 59 ; P < 0 . 0001 ) , IGF 2 ( AUC 0 . 66 ; P = 0 . 002 ) , and IGF 2 + IGFBP 3 ( AUC 0 . 67 ; P = 0 . 05 ) . ^^^ The combined measurement of free / total PSA , IGF 2 , and IGFBP 3 resulted in a slight improvement in performance ( AUC 0 . 76 ; P = 0 . 01 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A soft diet induced reductions of 25 76 % ( P < 0 . 05 0 . 01 ) in the expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 4 and IGFBP 6 compared with a hard diet , but induced a 25 % ( P < 0 . 05 ) increase only in expression of IGFBP 3 . ^^^ These findings suggest that the changes in expression of IGF 1 , IGF 2 , IGFR 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 are associated with the fibre type alteration of rat masseter muscle in response to diet consistency soon after weaning . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
AIM : To investigate the relationship between insulin like growth factor 1 , 2 ( IGF 1 and IGF 2 ) , IGF binding protein 3 ( IGFBP 3 ) and Child Pugh score in patients with liver cirrhosis , and to search for potential clinical markers of liver function . ^^^ Clinical significance of serum IGF 1 , IGF 2 and IGFBP 3 in liver cirrhosis . ^^^ Serum levels of IGF 1 , IGF 2 and IGFBP 3 were measured by immunoradiometric assay . ^^^ RESULTS : Serum IGF 1 , IGF 2 and IGFBP 3 levels were significantly lower in patients with cirrhosis than in controls , and serum concentrations of IGF 1 , IGF 2 and IGFBP 3 were associated with the severity of liver dysfunction , and dropped sharply during the progression of liver failure . ^^^ Concentrations of IGF 1 < 30 ng / mL , IGF 2 < 200 ng / mL and IGFBP 3 < 6 ng / mL implied a negative prognosis for patients with liver cirrhosis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The hIGF 2 transgene affects neither endogenous IGF 1 and IGF 2 expression , nor the expression of IGFBP 3 , which is located in the choroid plexus . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To investigate this observation further , we measured serum IGF 1 , IGF 2 and IGF binding protein 3 concentrations in 232 men and 210 women ( aged 55 64 years ) , and related peptide levels to smoking characteristics . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We determined serum levels of insulin like growth factors ( IGF 1 , IGF 2 ) and binding proteins ( IGFBP 2 and IGFBP 3 ) at diagnosis ( 1 ) , during ( 2 ) and after therapy ( 3 ) in 28 children treated for ALL . ^^^ RESULTS : 1 ) Serum absolute levels of IGF 1 , IGF 2 , IGFBP 3 rose and IGFBP 2 decreased during analysis . 2 ) Mean values of IGF 1 SDS were similar at diagnosis , during and after treatment ( 1 . 78+ / 0 . 9 vs . 1 . 86+ / 1 . 36 vs . 1 . 8+ / 0 . 83 ) . ^^^ IGF 2 SDS rose from 0 . 06+ / 1 . 45 ( 1 ) to 0 . 64+ / 1 . 4 ( 2 ) and to 0 . 83+ / 2 . 01 ( 3 ) and IGFBP 3 SDS increased from 0 . 23+ / 2 . 23 ( 1 ) to 0 . 92+ / 1 . 95 ( 2 ) and to 1 . 76+ / 2 . 43 ( 3 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGFBP 4 and IGFBP 6 levels were determined by RIAs . ^^^ RESULTS : NSD 1 ( + / ) patients showed significantly altered levels of IGF 1 ( mean 1 . 2 SDS ) , IGF 2 ( 1 . 2 ) , IGFBP 3 ( 1 . 7 ) , IGFBP 4 ( 0 . 4 ) , IGFBP 2 ( +0 . 8 ) and IGFBP 6 ( +1 . 5 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Maternal questionnaires were completed , and laboratory measurements of several variables , including IGF 1 , IGF 2 and IGF binding protein 3 ( IGFBP 3 ) , were made for a total of 331 apparently healthy full term newborns , from whom routine blood samples were taken during the first 5 days of their life . ^^^ In contrast , IGF 2 and IGFBP 3 had no effect on postnatal growth velocity . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Overexpression of COX 2 in NSCLC cells enhanced the antiapoptotic and mitogenic effects of IGF 1 and IGF 2 , facilitated the autophosphorylation of the type 1 IGF receptor , increased class IA phosphatidylinositol 3 ' kinase activity , and decreased expression of IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
EGF down regulated hypoxia induced IGF 2 binding protein 3 ( IGFBP 3 ) , which may contribute to the enhancement of free IGF 2 accessibility to its receptors in vivo . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : The odds ratios for endometrial cancer comparing the highest versus the lowest tertile of various IGF components were as follows : 0 . 5 [ 95 % confidence interval ( CI ) 0 . 2 1 . 2 ] for free IGF 1 , 1 . 1 ( 95 % CI 0 . 5 2 . 6 ) for total IGF 1 , 1 . 2 ( 95 % CI 0 . 6 2 . 6 ) for total IGF 2 , 2 . 4 ( 95 % CI 1 . 0 5 . 9 ) for IGF binding protein ( IGFBP ) 1 and 0 . 8 ( 95 % CI 0 . 4 2 . 0 ) for IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
All samples were analyzed for total and free IGF 1 and IGF 2 , IGFBP 3 and IGFBP 3 proteolysis , total and non phosphorylated ( np ) IGFBP 1 , and IGFBP 1 complexed IGF 1 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the relationship between levels of insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) , and insulin like growth factor binding protein 3 ( IGFBP 3 ) in antenatal maternal serum and in fetal cord blood at delivery . ^^^ RESULTS : There was no significant correlation between maternal IGF 1 , IGF 2 , or IGFBP 3 levels and fetal acidosis . ^^^ Maternal and fetal insulin like growth factors 1 and 2 ( IGF 1 , IGF 2 ) and IGF BP 3 , and their relationship to fetal acidosis at delivery . ^^^ Maternal IGF 1 , IGF 2 and IGF BP 3 were assessed on maternal blood at booking and in fetal blood by cord blood analysis at delivery . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MEASUREMENTS : We analysed the strength and independence of associations of angiographically assessed presence of CHD with BMI , systolic and diastolic blood pressure , total , high density lipoprotein ( HDL ) and LDL cholesterol , triglycerides , lipoprotein ( a ) , apolipoproteins A 1 and B , total and free IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 5 , acid labile subunit ( ALS ) , insulin , C peptide , testosterone , DHEAS and sex hormone binding globulin . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We examined the association between IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 3 protease activity and breast cancer risk in postmenopausal women , taking ER status of the breast cancer into consideration . ^^^ There was no association between IGF 1 , IGF 2 , IGFBP 2 , or IGFBP 3 and risk of ER negative breast cancer . ^^^ CONCLUSION : Serum IGFBP 3 and IGF 2 levels were positively associated with ER positive breast cancer risk . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
All four hormones increased gene and / or protein expression of PSA , IGF IR , IGF 1 , and IGFBP 2 and decreased that of AR , ERbeta , IGF 2 , and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Investigation of potential correlations between microarray expression data and genomic aberrations , detected by comparative genomic hybridization ( CGH ) , demonstrated that losses on chromosomes 10 and 14 were associated with distinct expression profiles , including increased expression of several genes related to the insulin like growth factor ( IGF ) ( IGF 2 , IGFBP 3 and AKT 3 ) or wingless ( WNT ) ( CTNNB 1 , CDK5R1 , ENC 1 and CCND 1 ) pathways . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied by real time reverse transcription PCR , whether there are differences in the abundance of mRNA coding for IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IGF 1R , IGF 2R , GHR , and InsR in compartmentalized layers ( fractions ) of jejunum and ileum of 5 d old calves fed colostrum . ^^^ In ileum , mRNA levels , expressed relative to housekeeping genes , differed ( P < 0 . 05 ) between fractions for IGF 1 ( LP > villi , crypts ) , IGF 2 , and IGFBP 3 ( villi > crypts , LP ) , GHR and InsR ( crypts > LP ) , IGFBP 2 ( crypts > villi , LP ) , and total RNA levels were greater ( P < 0 . 05 ) in LP and crypt than in villus fractions . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Total and free IGF 1 , IGF 2 , IGFBP 3 , and IGFBP 3 protease were similar between groups ( all P > . 5 ) , whereas , in LIPO , IGFBP 1 and IGFBP 2 were reduced ( 36 % , P < . 05 and 50 % , P < . 01 ) . ^^^ In pooled groups , total IGF 1 , free IGF 1 , total IGF 2 , and IGFBP 3 , respectively , correlated inversely with age ( all P < . 01 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In theca cells , IGF 2 decreased ( P < 0 . 05 ) IGFBP 2 mRNA levels , but had no effect on IGFBP 3 or 4 mRNA expression ( Exp . 3 ) ; IGF 1 did not affect ( P > 0 . 10 ) thecal IGFBP 2 , 3 or 4 mRNA levels . ^^^ Ligand blotting revealed that both IGF 1 and 2 increased IGFBP 2 and 5 ( protein ) and had no effect on IGFBP 3 ( protein ) , whereas IGF 1 ( but not IGF 2 ) increased IGFBP 4 ( protein ) , suggesting IGFBP 2 , 4 , and 5 are post transcriptionally regulated . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Free IGF 1 , total IGF 2 , IGFBP 3 , and GH binding protein were lower , whereas IGFBP 1 , IGFBP 1 bound IGF 1 , and IGFBP 2 were elevated compared with control values . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Blood samples were collected from the newborns no later than the fifth day of life for measurements of adiponectin and major IGF system components ( IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) ) . ^^^ In contrast , neonatal adiponectin levels correlate inversely significantly and independently with liver maturity and IGF 2 and tend to remain positively associated with IGFBP 3 and increased birth length . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGFBP 3 , IGFBP 4 and PAPP A mRNA expression and IGF 2 concentration did not differ ( P > 0 . 10 ) between dominant or subordinate follicles . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Interaction of insulin like growth factor 2 ( IGF 2 ) with multiple plasma proteins : high affinity binding of plasminogen to IGF 2 and IGF binding protein 3 . ^^^ Furthermore , an IGFBP 3 protease cleaving also IGFBP 2 but not IGFBP 4 was co purified from the IGF 2 column . ^^^ IGF 2 ligand blotting and surface plasmon resonance spectrometry have been used to identify plasminogen as a novel high affinity IGF 2 binding protein capable of binding to IGFBP 3 with 50 fold higher affinity than transferrin . ^^^ Size exclusion chromatography of the IGF 2 matrix eluate revealed that transferrin , plasminogen , and the IGFBP 3 protease are present in different high molecular mass complexes of > or =440 kDa . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 , IGFBP 2 , and IGFBP 3 were measured in 162 children with acute lymphoblastic leukemia treated by the Berlin Frankfurt Munster Study Group . ^^^ At diagnosis we found elevated IGFBP 2 , low IGFBP 3 , low IGF 1 , and low normal IGF 2 , but normal IGFBP 1 levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
BACKGROUND : Insulin like growth factor binding protein 3 ( IGFBP 3 ) is the chief binding protein for insulin like growth factors 1 and 2 ( IGF 1 and IGF 2 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF 1 was measured in serum and hearts by an ELISA method , cardiac IGF 1 and IGF 2 receptors and IGFBP 3 were measured by Western blotting analysis , and mRNAs for cardiac IGF 1 and IGF 2 were measured by RT PCR . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
A prospective study of serum insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 and breast cancer risk . ^^^ Neither IGF 1 nor IGFBP 3 was associated with risk in postmenopausal women and serum IGF 2 concentration was not associated with risk in pre or postmenopausal women . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : We examined serum concentrations of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein ( IGFBP ) 1 and IGFBP 3 , sex hormone binding globulin ( SHBG ) , leptin , fasting insulin , and blood glucose . ^^^ The mean serum concentrations of IGF 2 , IGFBP 1 , IGFBP 3 , SHBG , fasting insulin , blood glucose and HOMA IR did not differ between the SGA and the AGA group . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Combined treatment caused a higher increase in IGFBP 1 and larger suppression of total and free IGF 1 , IGF 2 , and IGFBP 3 ( P < 0 . 01 for all ) , but less suppression of IGFBP 2 ( P < 0 . 05 ) compared with tamoxifen monotherapy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Early developmental changes in IGF 1 , IGF 2 , IGF binding protein 1 , and IGF binding protein 3 concentration in the cerebrospinal fluid of children . ^^^ Herein , we have determined the concentrations of IGF 1 , IGF 2 , IGFBP 1 , and IGFBP 3 in cerebral spinal fluid ( CSF ) samples that were collected from children who were 1 wk to 18 y of age . ^^^ The concentrations of IGF 1 , IGFBP 1 , and IGFBP 3 in CSF from children < 6 mo of age were significantly higher than in older children , whereas IGF 2 was higher in the older group . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
OBJECTIVES : Epidermal Growth Factor Receptor ( EGF R ) up regulation in cervical cancer cells leads to an increase in cell proliferative Insulin like Growth Factor 2 ( IGF 2 ) and Vascular Endothelial Growth Factor ( VEGF ) and a decrease of the anti proliferative IGF binding protein 3 ( IGF BP 3 ) . ^^^ HPV E 6 ( measured only in HPV positive cells ) , EGF R , IGF 2 and IGF BP 3 levels were measured . ( d ) Cell proliferation was determined using cell proliferation Bradykinine U colorimetric assay , in HT 3 , HeLa and ME 180 cell lines in the presence of VEGF alone and with HPV E 6 antibodies . ^^^ RESULTS : ( a ) In all the HPV positive cell lines , 20 ng / ml VEGF significantly increased ( 30 50 % ; P < 0 . 0001 ) the HPV E 6 . ( b ) In the ME 180 and CaSki cells , VEGF treatment up regulated EGF R , IGF 2 and HPV E 6 and down regulated IGF BP 3 in a dose dependent manner ( P < 0 . 001 ) . ( c ) These effects of VEGF were eliminated when the HPV positive cells were co incubated with antibodies to HPV E 6 or EGF R . ^^^ In the HPV negative HT 3 cells , VEGF decreased IGF BP 3 while increasing EGF R and IGF 2 levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In addition , the PI 3 kinase inhibitor , LY 294002 , upregulates IGFBP 3 expression but downregulates IGF 1 and IGF 2 , indicating that PTEN controls IGFBP 3 and IGFs by an Akt dependent pathway . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Big IGF 2 suppresses growth hormone ( GH ) biosynthesis and impairs the storage of IGFs by suppressing the formation of the GH dependent ternary complexes containing IGF , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) . ^^^ Prednisone monotherapy partially suppressed big IGF 2 secretion , and rhGH monotherapy acted on IGFBP 3 and ALS concentrations . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The circulating levels of IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , and IGFBP 3 did not differ between patients and controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : To assess the degradation of frozen serum components in the JACC Study , we compared levels of various components ( IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , sFas , and total SOD activity ) between fresh and stored sera collected from other inhabitants . ^^^ Serum levels of constituents were measured by immunoradiometric assay ( IGF 1 , IGF 2 and IGFBP 3 ) , quantitative enzyme immunoassay ( TGF beta 1 ) , enzyme linked immuno adsorbent assay ( sFas ) , and an improved nitrite method ( SOD activity ) . ^^^ Levels of IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 and sFas in sera after storage for 9 years at 80 degrees C were similar to those of fresh sera newly collected from inhabitants . ^^^ The distributions of serum IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years . ^^^ CONCLUSIONS : There was no statistically significant difference in distribution of measured values of IGF 1 , IGF 2 , IGFBP 3 , TGF beta 1 , and sFas between newly collected sera and frozen specimens stored for 9 years . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IL 1beta , IL 6 , TNF alpha , IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , TSH , fT 3 and fT 4 were measured using standard commercial kits . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Infection with the human immunodeficiency virus ( HIV ) is often associated with a decrease of the concentrations of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and an increase of IGFBP 1 and 2 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum vascular endothelial growth factor C ( VEGF C ) as a specific biomarker for advanced cervical cancer : Relationship to insulin like growth factor 2 ( IGF 2 ) , IGF binding protein 3 ( IGF BP 3 ) and VEGF A [ corrected ] . ^^^ Here , we shall determine if serum levels of vascular endothelial growth factors B and C ( VEGF A [ corrected ] VEGF C ) associated with vasculogenic and lymphogenic metastasis may be used for an early diagnosis of advanced metastatic cervical cancer and compare these levels with those of the serum IGF 2 and IGF binding protein 3 ( IGF BP 3 ) . ^^^ There was a highly significant positive correlation between VEGF C and IGF 2 and a negative correlation between IGF BP 3 and VEGF C ( P < 0 . 0001 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No treatment effect was found for muscle IGF 1 ( P = 0 . 36 ) , IGF 2 ( P = 0 . 51 ) , IGFBP 3 ( P = 0 . 70 ) , or IGFBP 5 ( P = 0 . 51 ) mRNA abundance . ^^^ The abundance of IGF 1 ( P = 0 . 72 ) , IGF 2 ( P = 0 . 34 ) , and IGFBP 3 ( P = 0 . 99 ) in hepatic tissue was not influenced by treatment . ^^^ Uterine IGF 1 ( P = 0 . 46 ) , IGF 2 ( P = 0 . 40 ) , IGFBP 3 ( P = 0 . 29 ) , and IGFBP 5 ( P = 0 . 35 ) mRNA abundance did not differ between treatments . ^^^ Placental IGF 1 ( P = 0 . 30 ) , IGF 2 ( P = 0 . 18 ) , IGFBP 3 ( P = 0 . 94 ) , and IGFBP 5 ( P = 0 . 42 ) mRNA abundance did not differ between treatments . ^^^ The PEM isolated from sows fed L carnitine had decreased IGF 2 ( P = 0 . 02 ) , IGFBP 3 ( P = 0 . 03 ) , and myogenin ( P = 0 . 04 ; 61 , 59 , and 67 % , respectively ) mRNA abundance compared with controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Measures at baseline , 6 and 12 months included body weight , height , body fat , lean mass , body fat % , and waist circumference , as well as fasting glucose , insulin , insulin resistance , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , and IGF binding protein 1 , IGFBP 2 , and IGFBP 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We examined myocardial expression of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , IGF receptor 1 ( IGF 1R ) and IGF 2R in fetal sheep with maternal undernutrition . ^^^ At necropsy on day 78 or day 135 of gestation , gravid uteri were recovered . mRNA expression of IGF 1 and IGF 2 in ventricles were measured with RT PCR , and protein expression of IGF 1R , IGF 2R , IGFBP 3 was quantitated with Western blot . ^^^ No difference was found in IGF 1 , IGF 2 or IGFBP 3 levels between the NR and C groups at either gestational age . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There were no differences in plasma concentrations of total IGF 2 , free IGF 1 and 2 , IGFBP 3 , GHBP and ghrelin , whereas a small difference was found for IGFBP 2 ( 302+ / 97 vs . 263+ / 66 microg / L ; LNP vs . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Correlations between markers of inflammation and IGF 1 , IGF 2 and IGFBP 3 were examined in CD and UC patients in remission and relapse . ^^^ CONCLUSIONS : IGF 1 , IGF 2 and IGFBP 3 are correlated to albumin and IGF 1 and IGF 2 are correlated to CRP in IBD patients . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Inaccuracy of insulin like growth factor ( IGF ) binding protein ( IGFBP ) 3 assessment in the diagnosis of growth hormone ( GH ) deficiency from childhood to young adulthood : association to low GH dependency of IGF 2 and presence of circulating IGFBP 3 18 kilodalton fragment . ^^^ CONTEXT : Poor sensitivity of IGF binding protein ( IGFBP ) 3 assessment in the work up of GH deficiency ( GHD ) has been ascribed to the equal affinity of IGFBP 3 for IGF 1 and IGF 2 and to IGFBP 3 proteolysis . ^^^ OBJECTIVE : The objective of this study was to determine the IGF 2 GH dependency and IGFBP 3 proteolysis in patients with GHD from childhood to young adulthood . ^^^ INTERVENTIONS : IGF 1 , IGF 2 , and IGFBP 3 serum concentrations were measured by immunoradiometric assay . ^^^ MAIN OUTCOME MEASURES : Main outcome measures were sensitivity and specificity of IGF 1 , IGF 2 , and IGFBP 3 measurements . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This different growth rate correlated with growth hormone ( GH ) / insulin growth factor ( IGF ) axis dynamics : the donor had significantly lower insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor 2 ( IGF 2 ) and insulin like growth factor binding protein 3 ( IGFBP 3 ) values than the recipient on postoperative days ( POD ) 3 30 , although they had similar GH values . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
These complexes are referred to as binary complexes consisting of IGF 1 or IGF 2 and one IGFBP , or as ternary complexes each consisting of either of IGF 1 or IGF 2 , IGFBP 3 or 5 , and an acid labile subunit known as ALS . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : We conducted a prospective , nested case control analysis among apparently healthy men and women who were followed for up to 8 years in the Japan Collaborative Cohort ( JACC ) Study , to evaluate serum levels of insulin like growth factor 1 , 2 , and binding protein 3 ( IFG 1 , IGF 2 , and IGFBP 3 ) , transforming growth factorbeta 1 ( TGFbeta 1 ) , soluble fas ( sFas ) and superoxide dismutase activity ( SOD ) in 210 stomach cancer cases diagnosed in the JACC Study in relation to those levels in their 410 controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
No substantial associations were seen for IGF 1 and IGF 2 when also adjusting for IGFBP 3 ; adjusting IGFBP 3 for IGF 1 and 2 had only a minor impact on the risk estimates . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
There was no correlation of plasma IGFBP 4 level with age , sex , immunophenotype , or ALL risk group , and there was no correlation of IGFBP 4 level with plasma IGF 1 , IGF 2 , IGFBP 1 , IGFBP 2 , and IGFBP 3 levels . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We evaluated the relationship between plasma insulin , glucose , IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , apoptosis , and colorectal adenomas in a case control study . ^^^ Fasting plasma insulin , IGF 1 , IGF 2 , and IGFBP 3 levels were assessed by ELISA . ^^^ Overall , there were no significant differences between mean circulating levels of glucose , IGF 1 , IGF 2 , and IGFBP 3 among cases and controls and no association between these variables and apoptosis . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We assessed the association of prediagnostic plasma levels of IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 and subsequent occurrence of prostate cancer in a case control study nested in the SU . ^^^ Frozen baseline plasma samples were used to measure IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 . ^^^ After controlling for the intervention group in the trial and the other IGF axis variables , the odds ratios and 95 % confidence interval ( 95 % CI ) comparing the upper quartile to the baseline quartile were 1 . 83 ( 95 % CI , 0 . 85 3 . 95 ) , 1 . 05 ( 95 % CI , 0 . 35 3 . 18 ) , 0 . 79 ( 95 % CI , 0 . 39 1 . 58 ) , and 0 . 42 ( 95 % CI , 0 . 12 1 . 52 ) for IGF 1 , IGF 2 , IGFBP 2 , and IGFBP 3 , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We studied expression of the ligands IGF 1 and IGF 2 , the inhibitory IGF binding protein 3 , the type 1 IGF receptor ( IGF IR ) , and the downstream mediator insulin receptor substrate 1 by immunohistochemistry in 56 tissue specimens ( 28 low grade and 28 high grade prostate adenocarcinomas ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Changes in total IGF 1 ( tIGF 1 ) and total IGF 2 ( tIGF 2 ) , free IGF 1 ( fIGF 1 ) , IGF binding proteins ( IGFBP 1 and IGFBP 3 ) , IGFBP 3 protease activity and insulin levels were examined in 31 patients with active CD ( CDAI : 186 603 ) during treatment with prednisolone ( 40 mg for 1 week ) and tapering the dose by 5 mg / week . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In a rat osteoblast cell culture that we studied , IGF 2 and IGFBP 2 were the most abundant peptides of the IGF system released in cell medium , IGFBP 5 was abundantly expressed but bound to cell surface and cell matrix and IGF 1 and IGFBP 3 were found at very low concentrations . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Relationship between insulin like growth factors ( IGF 1 and IGF 2 ) , IGF binding proteins ( IGFBP 3 , IGFBP 2 ) , leptin and anthropometric parameters ( height , body mass index ) during antileukaemic treatment in children . ^^^ PURPOSE : The aim of the study was to estimate the anthropometric parameters and their relationship to serum levels of IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin before and during intensive antineoplastic treatment for acute lymphoblastic leukaemia in children . ^^^ MATERIAL AND METHODS : In 46 children in median age 6 . 6 years ( range from 1 . 6 to 16 ) we evaluated at the time of diagnosis , after protocol 1 and after intensive treatment , height , body mass index ( BMI ) and IGF 1 , IGF 2 , IGFBP 3 , IGFBP 2 and leptin . ^^^ IGF 1 SDS was low and similar at each point , IGF 2 SDS and IGFBP 3 SDS values augmented progressively and IGFBP 2 SDS was significantly elevated before treatment and lowered ( but not normalized ) during the therapy . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Among genes that were most significantly upregulated in carcinomas were 2 ubiquitin related genes ( USP 4 and UFD1L ) and several insulinlike growth factor related genes ( IGF 2 , IGF2R , IGFBP 3 and IGFBP 6 ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We investigated the actions of DHT , T , DHEA , and estradiol ( E 2 ) on insulin like growth factor ( IGF ) 1 , IGF 2 , IGF 1 receptor ( R ) , IGF binding protein ( IGFBP ) 2 , IGFBP 3 , and IGFBP 5 in primary cultures of human prostatic stromal cells by assessing cell proliferation , mRNA expression , and protein secretion by MTT growth assay , quantitative real time PCR , and ELISA , respectively . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 2 , IGFBP 3 , IL 1beta , IL 6 , TNFalpha , insulin and C peptide were measured . ^^^ Serum concentrations of IGF 1 and IGF 2 were significantly lower and insulin and C peptide levels significantly increased in CF compared with healthy controls whereas IGFBP 3 serum concentrations were similar , with comparable IGF I / IGFBP 3 and decreased IGF I / IGFBP 2 and IGF II / IGFBP 2 molar ratios . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : Human corneal fibroblasts were incubated with TGF beta 2 or IGF 1 , to investigate IGF 1 , IGF 2 , IGFBP 3 , type 1 collagen , and alpha smooth muscle actin ( alpha SMA ) mRNA , as well as IGFBP 3 protein expression , during myofibroblast differentiation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
DESIGN AND PARTICIPANTS : We analyzed data from 429 participants of the Boyd Orr cohort , for whom height measured in childhood ( mean age , 7 . 4 yr ) in 1937 1939 could be related to levels of IGF 1 , IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 in adulthood ( mean age , 71 . 1 yr ) . ^^^ Height in adulthood was not associated with IGF 1 , IGF 2 , or IGFBP 3 and was inversely associated with IGFBP 2 ( P = 0 . 05 ) after additionally controlling for childhood height . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In 27 children with AML ( mean age 13 . 6+ / 5 . 3 years ; patients in remission n=15 with relapse n=12 ) serum parameters of IGFBP 2 , IGFBP 3 , IGF 1 and IGF 2 were analyzed up to 18 months after HSCT by RIA . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
In the present study , circulating IGF 1 , IGF 2 and their binding proteins ( IGFBP 3 , IGFBP 2 and IGFBP 1 ) were determined in 92 adult patients with gastrointestinal inflammation ( Crohn ' s disease , colitis ulcerosa , gastritis , duodenitis errosiva , gastrointestinal candidiasis , and rotaviral and adenoviral enteritis ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Total RNA was extracted from the tumor and analyzed for IGF 2 , IGF binding protein 3 ( IGFBP 3 ) , and estrogen receptor alpha expressions using quantitative reverse transcription PCR . ^^^ The study also indicates that IGF 2 and IGFBP 3 have limited value in prognosis because of their strong associations with disease stage and tumor grade . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The mammographically dense area in the breast increased with increasing serum levels of IGF 1 ( P for linear trend , P ( t ) = 0 . 05 ) , IGF 2 ( P ( t ) = 0 . 08 ) , and IGFBP 3 ( P ( t ) = 0 . 01 ) only in premenopausal women . ^^^ IGF 2 and IGFBP 3 serum levels were associated with increases in the mammographically lucent area in both premenopausal ( P ( t ) = 0 . 01 and 0 . 04 , respectively ) and postmenopausal women ( P ( t ) < 0 . 001 for both ) , but these associations were no longer statistically significant after adjustment for body mass index and waist circumference . ^^^ The findings also show strong relations between IGF 2 and IGFBP 3 levels and the amount of mammographically lucent tissue , reflecting the associations between body adiposity and amount of fat tissue in the breast and between body adiposity and circulating levels of these growth factors . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Comparison of follicular fluid IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and PAPP A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF embryo transfer patients . ^^^ This is the first study that has compared follicular fluid ( FF ) IGF 1 , IGF 2 , IGFBP 3 , IGFBP 4 and pregnancy associated plasma protein ( PAPP ) A concentrations , and their ratios , to investigate whether there was any difference in the intrafollicular microenvironment between the GnRH agonist ( GnRHa ) and antagonist ( GnRHant ) protocols for controlled ovarian stimulation ( COS ) . ^^^ IGF 1 , IGF 2 and IGFBP 3 concentrations were measured by radioimmunoassay and IGFBP 4 and PAPP A by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
We report on a 10 yr old Caucasian girl affected by Costello syndrome with fasting hypoglycemia and short stature , associated low circulating levels of acid labile subunit ( ALS ) , relatively low levels of IGF 1 and IGFBP 3 , and normal IGF 2 , mostly circulating in a binary complex with IGFBP 2 and 6 instead of in a 150 kDa ternary complex . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
At baseline , GHD patients showed IGF 1 and IGF 2 levels and IGFs to IGFBP 3 molar ratios that were lower than controls . ^^^ These modifications resulted in normalization in IGF 1 to IGFBP 3 ratio , while no change in IGF 2 to IGFBP 3 ratio was observed . ^^^ In conclusion , the increase of serum IGF 2 levels during rhGH treatment in GHD patients probably reflects the increase in the other components of ternary complex ( ALS and IGFBP 3 ) . ^^^ However , serum IGF 2 levels as well as IGF 2 to IGFBP 3 ratio , although increased , were definitely lower than in controls . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
RESULTS : All the patients with certain GHD had low ( < or = 2 z score ) plasma concentrations of IGF 1 and ALS , but only 35 . 1 % had low IGF 2 , and 48 . 6 % had low IGFBP 3 . ^^^ All the patients but three ( 83 . 3 % ) with transient GHD had low IGF 1 , but only 44 . 4 % had low ALS , and only one had low IGF 2 or IGFBP 3 . ^^^ Almost all the patients with short stature and normal GH peak had normal serum IGF 2 and IGFBP 3 concentrations . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) is a multifunctional protein that regulates the potent mitogenic and antiapoptotic effects of IGF 1 and IGF 2 and exerts bioactivity independent of modulating IGF receptor activation . ^^^ IGF binding protein 3 ( IGFBP 3 ) is a multifunctional protein that regulates the potent mitogenic and antiapoptotic effects of IGF 1 and IGF 2 and exerts bioactivity independent of modulating IGF receptor activation . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
MAIN OUTCOMES : Outcomes included homeostasis model assessment of insulin resistance , total IGF 1 and IGF 2 , IGF binding protein ( IGFBP ) 2 , and IGFBP 3 in adulthood . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The changes in the serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF 2 , insulin like growth factor binding protein 3 ( IGBP 3 ) , estrogen , total alkaline phosphatase ( TALP ) , bone specific alkaline phosphatase ( BALP ) , calcium and phosphorous in serum , and also the histomorphology of the proximal tibia metaphysis and femur / body weight ( F / B ) ratio were examined in all the groups at every 10 days interval . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
METHODS : Fasting IGFBP 2 , IGFBP 1 , IGFBP 3 , IGF 1 , IGF 2 , insulin , C peptide , glucose , lipids , NEFAs , and HbA1c were measured in a cohort of 163 patients with type 2 diabetes . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Maternal and mixed umbilical cord blood was collected at delivery and analyzed for insulin , glucose , IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , GH , and GHBP . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
This study aimed to explore the effects of L arginine on the expression of insulin like growth factor ( IGF ) 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and IGF 1 mRNA in brains of IUGR rats and the possible mechanisms of L arginine . ^^^ The levels of IGF 1 , IGF 2 and IGFBP 3 in the brains of pup rats were measured by enzyme linked immunoadsordent assay ( ELISA ) and the expression of IGF 1 mRNA was detected by fluorescence quantitative PCR ( FQ PCR ) . ^^^ CONCLUSIONS : L arginine can increase the levels of IGF 1 and IGF 2 and the IGF 1 mRNA expression , and decrease the IGFBP 3 level in the brain of rats with IUGR induced by passive smoking , thereby offering protective effects against IUGR . . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
To assess whether circulating IGF 1 , IGF 2 , or IGF binding protein 3 ( IGFBP 3 ) were associated with cervical cancer and low grade and high grade squamous intraepithelial lesions ( LSIL and HSIL ) . ^^^ MATERIALS AND METHODS : Serum levels of IGF 1 , IGF 2 and IGFBP 3 were measured by ELISA . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Exogenous addition of IGFBP 3 markedly blocked IGF 1 and IGF 2 stimulated cell growth of KYN 2 and HepG 2 cells , and moderately stimulated that of KIM 1 and HAK 1B cells , but no growth of the KYN 1 , KYN 3 and HAK 1A cell lines was observed . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
When compared to a reference population the patient displayed : ( 1 ) An increased glucose disposal rate and a five fold elevation of forearm glucose uptake . ( 2 ) A decreased endogenous glucose production rate . ( 3 ) Decreased circulating levels of lipid intermediates . ( 4 ) Increased glucose oxidation and decreased lipid oxidation . ( 5 ) Low circulating levels of insulin like growth factor 2 and insulin like growth factor binding protein 3 and normal levels of insulin like growth factor 1 . ( 6 ) Normal insulin sensitivity ( euglycaemic glucose clamp ) . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
STUDY DESIGN : Plasma insulin like growth factor binding protein 3 concentrations were determined by iodine 125 insulin like growth factor 2 radioligand blots of nonreducing gels in 16 normal and 16 preeclamptic patients collected from a prospective , case control study . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
The prepubertal GH induced catch up growth was associated with elevated serum concentrations of insulin , insulin like growth factor 1 , insulin like growth factor binding protein 3 , and osteocalcin , whereas insulin like growth factor 2 levels remained unaltered . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
CONCLUSIONS : Critical illness is associated with low circulating concentrations of insulin like growth factor 1 , insulin like growth factor 2 , and insulin like growth factor binding protein 3 and these low values are associated with induction of protease activity specifically directed against insulin like growth factor binding protein 3 . ^^^
Interacting proteins: P01344 and P17936 Pubmed SVM Score :0.0
Effects of alpha interferon on insulin like growth factor 1 , insulin like growth factor 2 and insulin like growth factor binding protein 3 secretion by a human lung cancer cell line in vitro . ^^^