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NOS1AP Gene

protein-coding   GIFtS: 57

GC01P160306
nitric oxide synthase 1 (neuronal) adaptor protein
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
6330408P19Rik 2
CAPON 1, 2, 3, 5
KIAA0464 1, 3, 5
MGC138500 2
OTTHUMP00000025714 2
Descriptions
C--terminal PDZ ligand of neuronal nitric oxide synthase
protein 3
C-terminal PDZ domain ligand of neuronal nitric oxide
synthase (CAPON) 2
Nitric oxide synthase 1 adaptor protein 3
ligand of neuronal nitric oxide synthase with
carboxyl-terminal PDZ domain 2
nitric oxide synthase 1 (neuronal) adaptor protein 2
External Ids
HGNC: 168591
Entrez Gene: 97222
UniProtKB: O750523
Ensembl: ENSG000001989297
Search outside databases for aliases for NOS1AP gene

Previous GC identifer: GC01P158772

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NOS1AP:
This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide
synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with
nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G
protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein
functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the
synapsins. [provided by RefSeq]

UniProtKB/Swiss-Prot: CAPON_HUMAN, O75052
Function: Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its
association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific
NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1
and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for
interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1
and DLG4 (By similarity)

Gene Wiki entry for NOS1AP

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NOS1AP gene  

Entrez Gene cytogenetic band: 1q23.3   Ensembl cytogenetic band:  1q23.3   HGNC cytogenetic band: 1q23.3

NOS1AP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P160306:     (about GC identifiers)

Start:
160,306,205 bp from pter
End:
160,604,864 bp from pter
Size:
298,660 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CAPON_HUMAN, O75052 (See protein sequence)
Recommended Name: Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein  
Size: 506 amino acids; 56150 Da
Subunit: Interacts with the PDZ domain of NOS1 or the second PDZ domain of DLG4 through its
C-terminus. Interacts with RASD1 and SYN1, SYN2 and SYN3 via its PID domain. Forms a ternary
complex with NOS1 and RASD1. Forms a ternary complex with NOS1 and SYN1 (By similarity)
Secondary accessions: O43564 Q5VU95

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001119532.2  NP_055512.1  


    ENSEMBL proteins: 
    ENSP00000355133 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (CAPON)
    Human Recombinant Proteins from Abnova (NOS1AP)
                  OriGene Purified Recombinant Human Protein: NOS1AP 


    Antibodies for NOS1AP: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (CAPON), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NOS1AP)
    Novus Biologicals Antibody for NOS1AP

    Assays for NOS1AP: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR006020 PTB_PID
     IPR011993 PH_type


       GeneDecks  NOS1AP for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O75052

    ProtoNet protein and cluster: O75052

    UniProtKB/Swiss-Prot: CAPON_HUMAN, O75052
    Similarity: Contains 1 PID domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NOS1AP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001126060

                  Applied Biosystems Silencer® siRNAs for NOS1AP

                  Sigma-Aldrich siRNA for NOS1AP  
                         Sigma-Aldrich shRNA for NOS1AP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001126060
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001126060
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001126060 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014697

    UniProtKB/Swiss-Prot: CAPON_HUMAN, O75052
    Function: Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its
    association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific
    NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1
    and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for
    interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1
    and DLG4 (By similarity)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding ISS9459447
    GO:0030165 PDZ domain binding ISS9459447
    GO:0050998 nitric-oxide synthase binding ISS9459447
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  NOS1AP 


    5/10 Interacting proteins for NOS1AP (O750521 ENSP000003551333) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PTPRNQ168491EBI-780467, EBI-728153
    NOS1ENSP000003207583STRING (score=.967)
    SYN2ENSP000003429933STRING (score=.943)
    SYN1ENSP000002959873STRING (score=.941)
    SYN3ENSP000003302193STRING (score=.934)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NOS1AP
    1 Novoseek chemical compound relationship for NOS1AP gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    nitric oxide 51.42 18 18337493 (2), 19204306 (2), 15561099 (1), 18551039 (1) (see all 8)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NOS1AP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001126060

                  Sigma-Aldrich siRNA for NOS1AP  
                         Sigma-Aldrich shRNA for NOS1AP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001126060  NM_014697  

    REFSEQ mRNAs for NOS1AP gene (2 alternative transcripts): 

    NM_001126060.1   NM_014697.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001126060  NM_014697  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001126060
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001126060
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001126060 

    Additional cDNA sequence: 

    AB007933.2 AF037070.1 AK024942.1 AY841899.1 BC041382.2 BC112295.1 BC143771.1 BC143780.1 

    7 DOTS entries:

    DT.431228  DT.101013  DT.95260314  DT.91928393  DT.97822347  DT.121423665  DT.87016478 

    24/58 AceView cDNA sequences (see all 58 ):

    AI915161 AB007933 AF037070 AI150129 BX952349 NM_014697 BX281100 BM999232 
    AU131738 AA478926 AI802226 AI198232 AK125122 BG819229 AW136439 AW339403 
    CB306818 AI937609 CB054365 AU125189 AA129774 AI683194 AI559183 AA928879 

    highest scoring ESTs for NOS1AP:

    AB007933 AF037070 AI150129 AI198232 AU147312 BX281100 R19867 BC041382 AI797626 AY841899 

    Unigene Cluster for NOS1AP:

    Nitric oxide synthase 1 (neuronal) adaptor protein
    Hs.655000  [show with all ESTs]
    Unigene Representative Sequence: AY841899


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000361897  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NOS1AP expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NOS1AP

    1 / 2 / 3

    4 probe-sets matching NOS1AP gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    35531_at2, 3 U95-A 1 1.00 1.00 0.55 0.48 AB007933 1.00 0.80 0.91 1
    80768_at2, 3 U95-E 1 0.56 1.00 1.00 1.00 AI797626 0.40 1.00 0.76 1

    215153_at2, 3 U133-A 1 1.00 1.00 -- -- AF037070 0.80 0.88 0.84 1

    215153_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  NOS1AP for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGGTTTCTT

    SOURCE GeneReport for Unigene cluster: Hs.655000

    Expression variation in blood from EXPOLDB for NOS1AP

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NOS1AP gene from 4 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    rat
    (Rattus norvegicus)
    Nos1ap1   -- nitric oxide synthase 1 (neuronal) adaptor protein 90.15(n)
    95.01(a)
    192363  NM_138922.1  NP_620277.1 
    mouse
    (Mus musculus)
    Nos1ap1, 5 15
    nitric oxide synthase 1 (neuronal) adaptor protein1, 5 90.27(n)1
    95.72(a)1
    707291  NM_027528.21  NP_081804.11 
     AK0181495  AK0451245  (see all 11)
    chicken
    (Gallus gallus)
    NOS1AP1   -- nitric oxide synthase 1 (neuronal) adaptor protein 80.24(n)
    82.46(a)
    424371  XM_422215.2  XP_422215.2 
    African clawed frog
    (Xenopus laevis)
    Xl.305042   -- Xenopus laevis transcribed sequence with weak similarity more 80.21(n)  BG364067.1 
    About this table        Species with no ortholog for NOS1AP

    ENSEMBL Gene Tree for NOS1AP
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

    UniProtKB/Swiss-Prot: CAPON_HUMAN, O75052
    Polymorphism: Genetic variation in NOS1AP influences the electrocardiographic QT interval
    [MIM:610141]. The QT interval is defined as the time from the beginning of the Q wave to the end
    of the T wave, representing the duration of ventricular electrical activity. The QT interval, a
    measure of cardiac repolarization, is a genetically influenced quantitative trait with
    considerable medical relevance: both high and low values are associated with increased risk of
    cardiovascular morbidity and mortality


    10/1884 NCBI SNPs in NOS1AP are shown (see all 1884 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 759)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs9057201,2
    A,C,F,H,O160600724(+) CTACAC/TTGGTC 2 -- ut51 int116Minor allele frequency- T:0.34EU EA WA NA 1470
    rs9057211,2
    A,C,F,H,O160601676(+) ATGCCC/TATGCT 2 -- int1 ut5111Minor allele frequency- N:0.00NA EU WA EA 2248
    rs18769861,2
    A,C,F,H,O160605120(+) GAAAAG/ACTCCT 2 -- ut3115Minor allele frequency- A:0.37NA EU EA WA 1390
    rs109186021,2
    A,C,F,H160304324(+) CCTTTT/CTGTTT 1 -- ng518Minor allele frequency- C:0.31EU EA WA 830
    rs1641481,2
    A,C,F,H160605109(+) GGGGCG/AATGAG 2 -- ut314Minor allele frequency- A:0.23EU EA WA 420
    rs1641471,2
    A,C,F,H160605021(+) CACACC/ATATAA 2 -- ut3115Minor allele frequency- A:0.23NA EA EU WA 1302
    rs1641521,2
    A,C,F,H160605727(+) AGAACC/TAGGAA 2 -- ut314Minor allele frequency- T:0.25EU EA WA 420
    rs66597591,2
    A,C,F,H160304233(+) ATCTAT/CTATTA 1 -- ng51 tfbs34Minor allele frequency- C:0.43EU EA WA 370
    rs1641511,2
    A,C,F,H160605586(+) AAGAAT/CGAGGA 2 -- ut3114Minor allele frequency- C:0.24NA EA EU WA 1224
    rs120905851,2
    A,C,F,H160305400(+) TCTTAG/ACAATA 1 -- ng517Minor allele frequency- A:0.40NA EU EA WA 558
    About this table

    HapMap Linkage Disequilibrium images for NOS1AP (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605551   disorders: 610141  

    2 Novoseek disease relationships for NOS1AP gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    death sudden cardiac 60.46 2 19204306 (1)
    schizophrenia 40.74 25 19077434 (6), 16146415 (4), 15707951 (3), 18414510 (1)
    About this table

    Human Gene Mutation Database: NOS1AP
    Human Genome Epidemiology Navigator: NOS1AP (26 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/42 PubMed articles for NOS1AP gene (see all 42 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9722 HGNC: 16859 AceView: CAPON Ensembl:ENSG00000198929 euGenes: HUgn9722
    ECgene: NOS1AP H-InvDB: NOS1AP
    (According to HUGE)
    About This Section

    HUGE: KIAA0464
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NOS1AP:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NOS1AP-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NOS1AP Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NOS1AP Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Custom Service for Rabbit Pab from Full-length Protein
      
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     Recombinant Proteins
    (CAPON)
     Antibodies (CAPON)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for NOS1AP




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NOS1AP

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