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NAALADL1 Gene

protein-coding   GIFtS: 51

GC11M064569
N-acetylated alpha-linked acidic dipeptidase-like 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 3.4.17.21 3
I100 2, 3, 5
NAALADASEL 2, 3
NAALADL 3
Descriptions
100 kDa ileum brush border membrane protein 2, 3
Ileal dipeptidylpeptidase 2, 3
N-acetylated alpha-linked acidic dipeptidase-like 1 2
NAALADase L 3
External Ids
HGNC: 235361
Entrez Gene: 100042
UniProtKB: Q9UQQ13
Ensembl: ENSG000001680607
Search outside databases for aliases for NAALADL1 gene

Previous GC identifer: GC11M064588

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: NALDL_HUMAN, Q9UQQ1
Function: NAALADase-like activity unknown. Has no NAAG hydrolyzing activity. Exhibits a
dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAALADL1 gene  

Entrez Gene cytogenetic band: 11q12   Ensembl cytogenetic band:  11q13.1   HGNC cytogenetic band: 11q12

NAALADL1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M064569:     (about GC identifiers)

Start:
64,568,871 bp from pter
End:
64,582,585 bp from pter
Size:
13,715 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_033903.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NALDL_HUMAN, Q9UQQ1 (See protein sequence)
Recommended Name: N-acetylated-alpha-linked acidic dipeptidase-like protein  
Size: 740 amino acids; 80622 Da
Cofactor: Binds 2 zinc ions per subunit (By similarity)
Subcellular location: Apical cell membrane; Single-pass type II membrane protein. Note=Ileal brush
border membrane
Secondary accessions: O43176
Alternative splicing: 8 isoforms:  Q9UQQ1-1   Q9UQQ1-2   Q9UQQ1-3   Q9UQQ1-4   Q9UQQ1-5   Q9UQQ1-6   Q9UQQ1-7   Q9UQQ1-8   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005459.2  

    ENSEMBL proteins: 
    ENSP00000340111 ENSP00000347530 ENSP00000344244 ENSP00000351484 ENSP00000347955 ENSP00000349045 


    Human Recombinant Proteins 
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    Browse Abcam for Recombinant Proteins
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0016021 integral to membrane NAS9388249
    GO:0016324 apical plasma membrane IEA--
    About this table

    Antibodies for NAALADL1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    R&D Systems Antibodies for NAALADL1 (NAALADase-like 1/NAALADL1)
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for NAALADL1

    Assays for NAALADL1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR007484 Peptidase_M28
     IPR007365 Transferrin_rcpt-like_dimerise
     IPR003137 PA


       GeneDecks  NAALADL1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UQQ1

    ProtoNet protein and cluster: Q9UQQ1

    3 Blocks protein families:
    IPB003137 Protease-associated PA
    IPB007365 Transferrin receptor-like dimerisation domain
    IPB007484 Peptidase family M28


    UniProtKB/Swiss-Prot: NALDL_HUMAN, Q9UQQ1
    Similarity: Belongs to the peptidase M28 family. M28B subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAALADL1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005468

                  Applied Biosystems Silencer® siRNAs for NAALADL1

                  Sigma-Aldrich siRNA and siRNA Panels for NAALADL1  
                         Sigma-Aldrich shRNA for NAALADL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005468
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005468
                                     untagged cDNA clone in CMV expression vector: NM_005468 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005468

    UniProtKB/Swiss-Prot: NALDL_HUMAN, Q9UQQ1
    Function: NAALADase-like activity unknown. Has no NAAG hydrolyzing activity. Exhibits a
    dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC (By similarity)
    Catalytic activity: Release of an unsubstituted, C-terminal glutamyl residue, typically from
    Ac-Asp-Glu or folylpoly-gamma-glutamates
    Enzyme Number (IUBMB): EC 3.4.17.21 

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004180 carboxypeptidase activity IEA--
    GO:0008233 peptidase activity NAS9388249
    GO:0008237 metallopeptidase activity IEA--
    GO:0008270 zinc ion binding IEA--
    GO:0016805 dipeptidase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NAALADL1  (Your Favorite Gene powered by Ingenuity) 
     Lysine Degradation

       GeneDecks  NAALADL1 for the pathways selected above  
    About GeneDecksing


    1 Interacting protein for NAALADL1 (ENSP000003442443) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DPP4ENSP000003537313STRING (score=.808)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508 proteolysis IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAALADL1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAALADL1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005468

                  Sigma-Aldrich siRNA and siRNA Panels for NAALADL1  
                         Sigma-Aldrich shRNA for NAALADL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005468  

    REFSEQ mRNAs for NAALADL1 gene: 

    NM_005468.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005468  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005468
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005468
                                     untagged cDNA clone in CMV expression vector: NM_005468 

    Additional cDNA sequence: 

    AF001547.1 AF010141.1 AJ012371.1 AK309536.1 AK311090.1 BC143643.1 BC143644.1 

    7 DOTS entries:

    DT.401313  DT.120710133  DT.99972116  DT.40131683  DT.95154328  DT.95342236  DT.120710109 

    24/49 AceView cDNA sequences (see all 49 ):

    BU743082 CD514468 BP379466 BM726624 AI783670 AA504367 BX099491 AW170591 
    AI285158 AI244986 CB048466 BG757994 BU741137 AJ012371 BQ723610 AI868903 
    BP381256 BM682839 BF056631 BM719523 BM681269 AW135808 NM_005468 BF508363 

    highest scoring ESTs for NAALADL1:

    AJ012371 AF010141 AU098853 NM_005468 AA504367 AF001547 AI783670 AW024446 AW183158 BF111279 

    Unigene Cluster for NAALADL1:

    N-acetylated alpha-linked acidic dipeptidase-like 1
    Hs.13967  [show with all ESTs]
    Unigene Representative Sequence: NM_005468


    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for NAALADL1 (see all 10 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b · 9c · 9d ^ 10 ^ 11 ^ 12a · 12b · 12c ^ 13 ^ 14a · 14b ^ 15a · 15b · 15c ^ 16a · 16b · 16c ^
    SP1:                                                  -     -     -                       -     -                       -                                       
    SP2:                                                                                                        -           -                                       
    SP3:                                                                                -     -     -           -     -     -                                       
    SP4:                                                                                      -     -           -     -     -                                       
    SP5:                                                                                                                    -                                       

    ExUns: 17 ^ 18a · 18b · 18c
    SP1:                        
    SP2:                        
    SP3:                        
    SP4:                        
    SP5:                        

    About this scheme

    ECgene alternative splicing isoforms for NAALADL1

    6 Ensembl transcripts including schematic representations:
    ENST00000339885  ENST00000355369  ENST00000340252  ENST00000358658  ENST00000355721  ENST00000356632  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAALADL1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAALADL1

    1 / 2 / 3

    6 probe-sets matching NAALADL1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    34080_at2, 3 U95-A 1 1.00 1.00 0.87 0.53 AJ012371 1.00 1.00 1.00 1

    89057_at2, 3 U95-E 1 1.00 1.00 0.95 1.47 AI743674 0.60 0.75 0.68 1

    207895_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005468 0.60 1.00 0.82 1

    228424_at2, 3 U133-B 1 1.00 1.00 -- -- AW170591 0.40 0.67 0.55 1

    207895_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228424_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTGTGTATCC

    SOURCE GeneReport for Unigene cluster: Hs.13967

    Expression variation in blood from EXPOLDB for NAALADL1

    UniProtKB/Swiss-Prot: NALDL_HUMAN, Q9UQQ1
    Tissue specificity: Mainly expressed in the distal small intestine. Also expressed in the spleen
    and testis. Weak expression in the brain, locating mainly to the brain stem, amygdala, thalamus
    and ventral striatum. Isoforms 2 and 3 are found in the small intestine and colon

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAALADL1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAALADL11   -- N-acetylated alpha-linked acidic dipeptidase-like 1 80.64(n)
    80.73(a)
    483751  XM_540872.2  XP_540872.2 
    cow
    (Bos taurus)
    NAALADL11   -- N-acetylated alpha-linked acidic dipeptidase-like 1 84.3(n)
    83.22(a)
    506692  XM_583173.3  XP_583173.3 
    rat
    (Rattus norvegicus)
    Naaladl11   -- N-acetylated alpha-linked acidic dipeptidase-like 1 81.44(n)
    80.95(a)
    83568  NM_031759.1  NP_113947.1 
    mouse
    (Mus musculus)
    Naaladl11, 5 195
    N-acetylated alpha-linked acidic dipeptidase-like 11, 5 81.08(n)1
    81.89(a)1
    3812041  NM_001009546.11  NP_001009546.11 
     AC1311145  BC1485255  (see all 6)
    zebrafish
    (Danio rerio)
    CH211-214J8.11   -- N-acetylated alpha-linked acidic dipeptidase-like 1 57.81(n)
    56.71(a)
    100000223  NM_001098190.1  NP_001091660.1 
    About this table        Species with no ortholog for NAALADL1

    ENSEMBL Gene Tree for NAALADL1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NAALADL1 gene
    FOLH12  ENSG000001346122  NAALAD22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/67 NCBI SNPs in NAALADL1 are shown (see all 67 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs37590411,2
    C,F,H64583663(-) ATGACA/GGAGTC 1 -- ng3110Minor allele frequency- G:0.34EA NA EU WA 1512
    rs122239861,2
    C,F,H64577271(+) CCTTCT/GTCAGC 1 T/K mis1 ese35Minor allele frequency- G:0.10EU EA WA 590
    rs354225061,2
    C,F64582564(+) GTCCAC/GTGCAT 1 Q/H mis15Minor allele frequency- G:0.01NA EU EA WA 432
    rs360533401,2
    C,F64570261(+) CTCCAG/CCAGGG 1 V/L mis11Minor allele frequency- C:0.05NA 60
    rs343884711,2
    F64581533(+) TAGACG/AAGGAG 1 L/L syn11Minor allele frequency- A:0.07NA 46
    rs4775391,2
    A,C,F,H64577230(+) GGGGGC/TGAAGG 1 -- int16Minor allele frequency- T:0.16EU EA WA 2076
    rs4886861,2
    A,C,F,H64571001(+) TAATCA/GATGCT 1 -- int15Minor allele frequency- G:0.38MN EU EA 654
    rs13008451,2
    A,C,F,H64575744(+) CAGCAA/GTGGCG 1 -- int117Minor allele frequency- N:0.00EU EA WA NA 1580
    rs22984051,2
    C,F,H64581314(+) GTTCCC/TCACTC 1 -- int110Minor allele frequency- N:0.00EA EU WA NA 2288
    rs5662211,2
    A,C,F64576859(-) TCAGCA/GTTTCA 1 -- int112Minor allele frequency- G:0.47EA NA MN 873
    About this table

    HapMap Linkage Disequilibrium images for NAALADL1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602640

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    3 PubMed articles for NAALADL1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10004 HGNC: 23536 AceView: NAALADL1 Ensembl:ENSG00000168060 euGenes: HUgn10004
    ECgene: NAALADL1 H-InvDB: NAALADL1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAALADL1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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